Determinants Of Virus Variation, Evolution, And Host Adaptation,
2022
University of Nebraska-Lincoln
Determinants Of Virus Variation, Evolution, And Host Adaptation, Katherine Latourrette, Hernan Garcia-Ruiz
Nebraska Center for Virology: Faculty Publications
Virus evolution is the change in the genetic structure of a viral population over time and results in the emergence of new viral variants, strains, and species with novel biological properties, including adaptation to new hosts. There are host, vector, environmental, and viral factors that contribute to virus evolution. To achieve or fine tune compatibility and successfully establish infection, viruses adapt to a particular host species or to a group of species. However, some viruses are better able to adapt to diverse hosts, vectors, and environments. Viruses generate genetic diversity through mutation, reassortment, and recombination. Plant viruses are exposed to …
Immunogenicity And Protective Efficacy Of A Recombinant
Pichinde Viral-Vectored Vaccine Expressing Influenza Virus
Hemagglutinin Antigen In Pigs,
2022
University of Nebraska-Lincoln
Immunogenicity And Protective Efficacy Of A Recombinant Pichinde Viral-Vectored Vaccine Expressing Influenza Virus Hemagglutinin Antigen In Pigs, Sushmita Kumari, Jayeshbhai Chaudhari, Qinfeng Huang, Phillip Gauger, Marcelo Nunes De Almeida, Yuying Liang, Hinh Ly, Hiep Vu
Nebraska Center for Virology: Faculty Publications
Influenza A virus of swine (IAV-S) is an economically important swine pathogen. The IAV-S hemagglutinin (HA) surface protein is the main target for vaccine development. In this study, we evaluated the feasibility of using the recombinant tri-segmented Pichinde virus (rPICV) as a viral vector to deliver HA antigen to protect pigs against IAV-S challenge. Four groups of weaned pigs (T01–T04) were included in the study. T01 was injected with PBS to serve as a non-vaccinated control. T02 was inoculated with rPICV expressing green fluorescence protein (rPICV-GFP). T03 was vaccinated with rPICV expressing the HA antigen of the IAV-S H3N2 strain …
Inhibition Of Staphylococcus Aureus Biofilm By Variovorax Paradoxus,
2022
University of the Pacific
Inhibition Of Staphylococcus Aureus Biofilm By Variovorax Paradoxus, Esther Gomez
University of the Pacific Theses and Dissertations
Staphylococcus aureus is one of the leading causes of fatal nosocomial infections. Often, S. aureus can grow as a biofilm which protects the population from the surrounding environment. Strains of S. aureus are resistant to virtually all known antibiotics on the market. Variovorax paradoxus is a soil microbe with many unusual metabolic activities. It has been previously observed that, V. paradoxus can inhibit the growth of S. aureus when in co-culture. In this work we report on inhibition of S. aureus biofilm formation by V. paradoxus due to a suspected inhibitory soluble factor.
Transitions In Bacterial Communities Across Two Fermentation-Based Virgin Coconut Oil (Vco) Production Processes,
2022
Ateneo de Manila University
Transitions In Bacterial Communities Across Two Fermentation-Based Virgin Coconut Oil (Vco) Production Processes, Zomesh A N Maini, Crisanto M. Lopez
Biology Faculty Publications
Despite being one of the most used methods of virgin coconut oil (VCO) production, there is no metagenomic study that details the bacterial community shifts during fermentation-based VCO production. The identification and quantification of bacteria associated with coconut milk fermentation is useful for detecting the dominant microbial genera actively involved in VCO production which remains largely undescribed. Describing the constitutive microbial genera involved in this traditional fermentation practice can be used as a preliminary basis for improving industrial practices and developing better fermentation procedures. In this study, we utilized 16S rRNA metagenomic sequencing to trace the transitions in microbial community …
Antibacterial Activity Of Commiphora Molmol (Myrrha) Against The Periodontal Pathogen, Aggregatibacter Actinomycetemcomitans,
2022
Long Island University
Antibacterial Activity Of Commiphora Molmol (Myrrha) Against The Periodontal Pathogen, Aggregatibacter Actinomycetemcomitans, Khalid Nafea Alharbi
Selected Full-Text Master Theses 2021-
The exogenous pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is most frequently involved in periodontitis and other systemic diseases. The treatment of these infections involves antibiotic therapy with Amoxicillin being the most popular antibiotic against A. actinomycetemcomitans but now, the drug is not 100% effective due to the development of 0-84% antibiotic resistance. The present study aimed to determine antibacterial activity of Commiphora molmol (Myrrha) against the periodontal pathogen, Aggregatibacter actinomycetemcomitans. In detail, the strain LIU1239 of Aggregatibacter actinomycetemcomitans was used where the plasmid pVJT128 is used for transposon mutagenesis and the Escherichia coli strain MV10Nal (stock # LIU4). The …
Copper(Ii) And Silver(I)‑1,10‑Phenanthroline‑5,6‑Dione Complexes Interact With Double‑Stranded Dna: Further Evidence Of Their Apparent Multi‑Modal Activity Towards Pseudomonas Aeruginosa,
2022
Universidade Federal do Rio de Janeiro
Copper(Ii) And Silver(I)‑1,10‑Phenanthroline‑5,6‑Dione Complexes Interact With Double‑Stranded Dna: Further Evidence Of Their Apparent Multi‑Modal Activity Towards Pseudomonas Aeruginosa, Anna Clara Milesi Galdino, Lívia Viganor, Matheus Mendonça Pereira, Michael Devereux, Malachy Mccann, Marta Helena Branquinha, Zara Molphy, Sinéad O'Carroll, Conor Bain, Georgia Menounou, Andrew Kellett, André Luis Souza Dos Santos
Articles
Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)2]ClO4 (Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)3](ClO4)2.4H2O (Cu-phendione), with efficient antimicrobial action against multidrug-resistant species. Herein, we investigated the ability of Ag-phendione and Cu-phendione to bind with double-stranded DNA using a combination of in silico and in vitro approaches. Molecular docking revealed that both phendione derivatives can interact with the DNA by hydrogen bonding, hydrophobic and electrostatic interactions. Cu-phendione exhibited the highest binding affinity to either major (− 7.9 kcal/mol) or minor (− 7.2 kcal/mol) …
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication,
2022
South Dakota State University
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal
Electronic Theses and Dissertations
In this study, the role of IFITM3 on PRRSV replication was studied in vitro by expressing exogenous IFITM3 in MARC-145 cells. An average of 31% reduction in PRRSV N protein expression and an average of 5.4 fold decrease in virus titer in the supernatant were observed in IFITM3 overexpressing cells as compared to vector control cells at 24 hours post infection (hpi). Moreover, there was a positive correlation between interferon- induced IFITM3 up-regulation and reduced PRRSV replication. To determine the role of endogenous IFITM3 in PRRSV replication, siRNA induced knockdown of IFITM3 was employed. RT-PCR validated the successful silencing of …
Decoding The Mystery Of Antibiotic Persistence,
2022
South Dakota State University
Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain
Electronic Theses and Dissertations
This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …
The Bgs 13 Mutant Of Pichia Pastoris And Its Effect On Structural Changes Of The Reporter Protein B-Lactogolbulin,
2022
University of the Pacific
The Bgs 13 Mutant Of Pichia Pastoris And Its Effect On Structural Changes Of The Reporter Protein B-Lactogolbulin, Bushra Irshad
University of the Pacific Theses and Dissertations
Pichia pastoris, a methylotrophic yeast, is an ideal host for recombinant protein expression. It has the capability of performing many eukaryotic post-translational modifications and grows to high cell densities. However, P. pastoris’s secretion properties are not always efficient, and its secretory pathway mechanisms have not been thoroughly elucidated. A previously identified mutant strain, bgs13, was found to efficiently secrete most recombinant proteins tested, raising the possibility that this bgs13 mutant is a universal super secreter and understanding its secretion process is needed. In this study, we used a reporter protein, ?-lactoglobulin (b-LG), to perform structural analysis and comparisons of protein …
The Role Of The Skin Microbiome In Amphibian Pathogen Susceptibility In Turnbull National Wildlife Refuge,
2022
Eastern Washington University
The Role Of The Skin Microbiome In Amphibian Pathogen Susceptibility In Turnbull National Wildlife Refuge, Krista S. Dodd
EWU Masters Thesis Collection
The fungal pathogen, Batrachochytrium dendrobatidis (Bd), has led to the decline and extinction of many amphibian populations, but some bacteria in the skin microbiome can inhibit its growth. In Turnbull National Wildlife Refuge (TNWR) in eastern Washington, Bd is highly prevalent, but the role of the skin microbiome in Bd infection dynamics have not been examined in this region. We hypothesized that frogs with lower Bd infection intensities would have higher skin bacterial diversity and more abundant anti-Bd bacteria, indicative of a more protective function. Our study combined cultureindependent and culture-dependent methods to assess the relationship between Bd and the …
Botanical Inventory And Management Consideration For Potential Park At 32 Nd Street Property: City Of Grand Rapids Parks And Recreation,
2022
Calvin University
Botanical Inventory And Management Consideration For Potential Park At 32 Nd Street Property: City Of Grand Rapids Parks And Recreation, David Warners, William Hofmann, Hayden Janssen, Martin Vanderschoot, Garrett Crow
Faculty and Professional Research
During the spring and summer of 2022, a botanical inventory and floristic quality assessment was carried out by Calvin University students and faculty for the 32 nd Street Property proposed as a 17-acre potential City Park, located at 2163 32 nd Street SE in Grand Rapids, Michigan. The purpose of this inventory was to inform the staff of the Grand Rapids Parks and Recreation Department of the botanical significance of this newly sectioned property and also to provide information for the City to share with neighbors of the plants that could be found in this parcel. The research conducted as …
Microbial Community And Soil Responses To Land Management Practices In Remnant And Restored Tallgrass Prairies,
2022
Northern Illinois University
Microbial Community And Soil Responses To Land Management Practices In Remnant And Restored Tallgrass Prairies, Desirae Marie Klimek
Graduate Research Theses & Dissertations
Critical ecosystem functions, such as decomposition and nutrient cycling, are driven by microbial communities within soil. As such, it is important to examine the effect of restoration practices, such as the presence of native grazers and prescribed burning, on these microbes and the soil they inhabit. The Nachusa Grasslands provides a chronosequence of restored tallgrass prairies ranging in restoration age from 5 to 33 years, as well as remnant prairies, and agricultural fields. These sites were sampled seasonally from 2013-2020 and microbial ribosomal RNA genes were surveyed to characterize soil microbial communities and assess how common restoration practices affect these …
Impact Of Prairie Restoration On Geochemistry And Microbial Communities In Groundwater,
2022
Northern Illinois University
Impact Of Prairie Restoration On Geochemistry And Microbial Communities In Groundwater, Kayla Koenig
Graduate Research Theses & Dissertations
Prairie restoration is important for reversing the loss of biodiversity and repairing ecosystem functions humanity is dependent on diverse ecosystems. This study looks at the impact of the beginning phases of prairie restoration on groundwater geochemistry and microbial communities the relationship between them. This research project studied the geochemistry and microbial communities in five wells before, during, and after the beginning phases of the first year of a prairie restoration on the Northern Illinois University campus. Water samples were collected bimonthly for a year and analyzed on an Ion Chromatograph. Microbial samples were collected monthly and underwent DNA extraction, amplification, …
Characterization And Quantification Of Far Red Oxygen Production Rates In Acaryochloris Spp.,
2022
Northern Illinois University
Characterization And Quantification Of Far Red Oxygen Production Rates In Acaryochloris Spp., Jose Manuel Orench-Benvenutti
Graduate Research Theses & Dissertations
Oxygenic photosynthesis is one of the primary metabolisms on Earth responsible for the evolution of complex life. Its advent around the Archean steadily increased Earth’s atmospheric oxygen from negligible amounts to biologically impressive quantities. Atmospheric oxygen is the sole result of billions of years of biological oxygenation and no abiotic process can account for its high presence in the Earth’s atmosphere. Understanding its origins and evolution can lead us to better understand Earth’s biological history and the possibility of analogues elsewhere. A research path that could lead to answers is comparing distinct incorporations of photopigments in various oxygenic phototrophs. In …
Antibiotic Permeation In Gram-Negative Bacteria And Contribution Of Inflammasome Activation And Pyroptosis In Pathogenesis Of Salmonella Systemic Infection,
2022
University of Kentucky
Antibiotic Permeation In Gram-Negative Bacteria And Contribution Of Inflammasome Activation And Pyroptosis In Pathogenesis Of Salmonella Systemic Infection, Ankit Pandeya
Theses and Dissertations--Chemistry
Antibiotic resistance is one of the major global issues in the field of public health and medicine. Good antibiotic candidates need to be selectively toxic, inhibit cellular target, and effectively penetrate and accumulate in bacterial cells. The last factor is a formidable barrier in the development of antimicrobials effective in Gram-negative bacteria, due to the presence of two layers of cell envelope. The first half of my thesis focuses on understanding the permeation of small molecules through this formidable cell envelope, distribution inside the cell of Gram-negative bacteria, and design of novel methods to make small molecules effectively cross the …
Alternative Splicing Regulates The Innate Immune Response To Viral Infection,
2022
University of Montana, Missoula
Alternative Splicing Regulates The Innate Immune Response To Viral Infection, Luke A. White
Graduate Student Theses, Dissertations, & Professional Papers
Rift Valley fever virus (RVFV) is a mosquito-borne RNA virus that infects humans and livestock in sub-Saharan Africa and the Arabian peninsula, causing disease ranging from a mild flu-like illness to liver damage, blindness, hemorrhagic fever, death, and, especially in livestock animals, high rates of abortive pregnancies. There is no approved vaccine for RVFV, and as a disease with a high rate of spread that causes severe illness, it is listed as a Category A pathogen by the USA CDC. A better understanding of RVFV’s molecular virology will be instrumental to combating RVFV as climate change causes its mosquito host …
Microbial Community Analysis: Biofilm Inhibition & Algae Associated Community Structure,
2022
University of the Pacific
Microbial Community Analysis: Biofilm Inhibition & Algae Associated Community Structure, Michelle V. Fong
University of the Pacific Theses and Dissertations
Natural products chemistry is the pursuit of bioactive small molecules from living organisms. These can be classified as primary metabolites if they are essential to survival, and secondary metabolites if they are accessory, playing a role in communication, defense, recruitment, etc.. Natural products have made a significant contribution to society – of 1,881 FDA-approved drugs from 1981 to 2019, 4% were pure natural products, 19% were natural products derived, and 3% were synthetic drugs with a natural products pharmacophore targeting a wide range of diseases and infections (Newman & Cragg, 2020). Pharmacophores are structural components of drugs that are responsible …
Bisacodyl Limits Chikungunya Virus In Vitro And Is Broadly Antiviral,
2022
Loyola University Chicago
Bisacodyl Limits Chikungunya Virus In Vitro And Is Broadly Antiviral, Natalie June Lomascolo
Master's Theses
Identifying novel antivirals requires significant time and resource investment, and the continuous threat of viruses to human health necessitates commitment to antiviral identification and development. Developing antivirals requires years of research and validation, and recent outbreaks have highlighted the need for preparedness in counteracting pandemics. One way to facilitate development is to repurpose molecules already used clinically. By screening such compounds, we can accelerate antiviral development. Here, we screened compounds from the National Institutes of Health’s Developmental Therapeutic Program for activity against chikungunya virus, an alphavirus that is responsible for a significant outbreak in the Americas in 2013. Using this …
Investigating The Role Of Nagc Reveals It To Be A Global Regulator,
2022
Loyola University Chicago
Investigating The Role Of Nagc Reveals It To Be A Global Regulator, Natasha Peterson
Master's Theses
Bacterial colonization of host surfaces can result in symbiotic or pathogenic associations.As bacteria becomes more resistant to antibiotics, pathogenic bacterial colonization poses an increasing risk to human health. Symbiotic associations that have beneficial outcomes for both the host and the microbe can serve as models to investigate how bacterial processes affect colonization of host surfaces. The symbiotic relationship between the Gram-negative, bioluminescent bacterium Vibrio fischeri and the Hawaiian bobtail squid is one such model (E. V. Stabb & Visick, 2013). The study of this exclusive relationship has developed in a way where in vitro phenotypes can predict the ability of …
Calcium-Mediated Induction Of Vibrio Fischeri Es114 Biofilms,
2022
Loyola University Chicago
Calcium-Mediated Induction Of Vibrio Fischeri Es114 Biofilms, Katia Elizabeth Semenchuk
Master's Theses
Vibrio fischeri bacteria form biofilms that facilitate symbiotic colonization of the Hawaiian bobtail squid, Euprymna scolopes. These host-associated biofilms require production of the SYP polysaccharide. However, it has been difficult to observe robust biofilm formation in vitro in a laboratory setting using wild-type strain ES114; instead, most work investigating syp-dependent biofilm formation has relied on genetically altered strains. Guided by recent findings that increasing calcium induces c-di-GMP (Tischler et al., 2021), a molecule known in other systems to promote biofilm formation, the question was posed as to whether high levels of calcium alone could promote syp-dependent biofilm formation by ES114. …
