The Now Trial: A Pragmatic Randomized Controlled Trial Of Personalized, Genetic-Based Lifestyle Advice, 2020 The University of Western Ontario
The Now Trial: A Pragmatic Randomized Controlled Trial Of Personalized, Genetic-Based Lifestyle Advice, Justine Rochelle Horne
Electronic Thesis and Dissertation Repository
Background: The impact of nutrigenomics and lifestyle genomics interventions on health outcomes and behaviours remains controversial and under-explored.
Objectives: To determine the short-term (3-month), moderate-term (6-month) and long-term (12-month) impact of providing personalized, genetic-based lifestyle information and advice on anthropometric measures, as well as dietary intake and adherence.
Methods: The nutrigenomics, overweight/obesity and weight management trial (NOW Trial) is a pragmatic randomized controlled trial that was incorporated into the Group Lifestyle Balance™ (GLB) program (N=140). Inclusion criteria: overweight or obesity (BMI ≥ 25 kg/m2), ≥ 18 years of age, English-speaking, having access to internet at least …
Analyzing The Effect On Ligand Sensitivity For Xenopus Tropicalis Mc1r, Mc3r, Mc4r, And Mc5r As A Result Of Coexpression With Gallus Gallus Mrap1 In Chinese Hamster Ovary Cells, 2020 University of Denver
Analyzing The Effect On Ligand Sensitivity For Xenopus Tropicalis Mc1r, Mc3r, Mc4r, And Mc5r As A Result Of Coexpression With Gallus Gallus Mrap1 In Chinese Hamster Ovary Cells, Avery Niemann, Robert Dores
DU Undergraduate Research Journal Archive
Some melanocortin receptors and the accessory protein MRAP1 have been found to interact in novel ways when co-expressed in the same cells following stimulation with either ACTH (1-24) or αMSH. These interactions have been seen for mammalian, bird, and some bony fish melanocortin receptors. Such analysis has not been done yet on the amphibian melanocortin receptors, MC1R, MC3R, MC4R, and MC5R. To this end, this study was done on the effects regarding ligand sensitivity for ACTH (1-24) and αMSH, when the MC1R, MC3R, MC4R, and MC5R paralogs of the amphibian, Xenopus tropicalis were expressed in Chinese Hamster Ovary cells either …
Landscape Genetics Of Salamander Populations At Mammoth Cave National Park, 2020 Western Kentucky University
Landscape Genetics Of Salamander Populations At Mammoth Cave National Park, Jackson Resser
Mahurin Honors College Capstone Experience/Thesis Projects
Habitat connectivity affects the distribution of genetic diversity among populations by influencing the movements of individuals and the resulting pattern of gene flow across landscapes. It has become evident that amphibians are experiencing a period of worldwide population declines brought about by environmental change. An understanding of the effects of habitat structure on landscape connectivity is important for developing effective amphibian conservation strategies. The purpose of this study is to investigate the effect of landscape characteristics on gene flow and population structure of the marbled salamander (Ambystoma opacum) in Mammoth Cave National Park, Kentucky, USA. Salamander larvae were …
Validation Of Ninein As An Ethanol-Related Quantitative Trait Gene: Reassessment, Design, And Functional Validation Of Reference Genes For Qpcr Analysis Of Brain Tissue In Mice, 2020 Virginia Commonwealth University
Validation Of Ninein As An Ethanol-Related Quantitative Trait Gene: Reassessment, Design, And Functional Validation Of Reference Genes For Qpcr Analysis Of Brain Tissue In Mice, Jessica L. Jurmain
Theses and Dissertations
The increasing use of quantitative real-time polymerase chain reaction (qPCR) as a method for quantifying gene expression has led to an increased demand for standardization of data analysis methods to ensure accurate reporting and robust, reproducible results. The exponential nature of qPCR amplification results in the potential magnification of what are usually very small sources of error. Relative gene expression calculations circumvent this issue by normalizing target gene expression data to within-sample expression of a previously validated, stably expressed reference gene or genes. Multiple studies discussed herein have found that qPCR data are more reliable and reproducible when multiple reference …
Spatial And Temporal Genetic Structure Of Winter-Run Steelhead (Oncorhynchus Mykiss) Returning To The Mad River, California, 2020 Humboldt State University
Spatial And Temporal Genetic Structure Of Winter-Run Steelhead (Oncorhynchus Mykiss) Returning To The Mad River, California, Steven R. Fong
Cal Poly Humboldt theses and projects
Distinct populations of steelhead in the wild are in decline. The propagation of steelhead in hatcheries has been used to boost population numbers for recreational fisheries and for use in conservation. However, hatchery breeding practices of steelhead can result in changes in genetic structure. I investigated the genetic structure of winter-run steelhead (Oncorhynchus mykiss) returning to the Mad River, California, where a hatchery has been used enhance production for recreational fisheries since 1971. Genetic variability in Mad River steelhead was evaluated using 96 single nucleotide polymorphisms (SNPs) among 4203 individuals, including the Mad River and nearby locations, and …
Floral Trait Architecture In Crop Sunflower (Helianthus Annuus) Under Drought Conditions, 2020 Central Washington University
Floral Trait Architecture In Crop Sunflower (Helianthus Annuus) Under Drought Conditions, Lauren Segarra
All Master's Theses
Longer and more intense droughts are predicted to become more common in the coming century due to anthropogenic climate change. Drought can reduce crop yield and decrease food security. In order to mitigate the negative effects of drought on crop production, it is important to elucidate the underlying mechanisms that promote drought stress resistance in crop plants. Floral traits impact yield, especially in oilseed crops such as sunflower (Helianthus annuus), but their susceptibility to drought stress is understudied. The goal of this study was to describe the floral trait architecture of H. annuus crop lines under drought versus …
Analysis Of Changes In Genes That Lead To Antibiotic Resistance In Klebsiella Pneumoniae 43816, 2020 University of North Florida
Analysis Of Changes In Genes That Lead To Antibiotic Resistance In Klebsiella Pneumoniae 43816, Jasmine R. Anderson
UNF Graduate Theses and Dissertations
Klebsiella pneumoniae is a Gram-negative bacterium responsible for nosocomial infections such as blood stream infections, meningitis, and septicemia. Clinical isolates confirm K. pneumoniae is increasingly antibiotic resistant (AR) making treatment more difficult. While bacterial exposure to antimicrobial substances is a known cause of the AR phenotype, there still exists gaps in knowledge about the genetic changes responsible for this fitness change. The laboratory Klebsiella pneumoniae 43816 (ATCC 43816) was exposed to sublethal concentrations of Cephalothin for a 14-day period. At the end of the experimental treatment, the adapted population acquired clinical antibiotic resistance along with phenotypic and morphological changes. The …
Error In Dna Mixture Analysis: Why The Application Of Forensic Dna Is Not A Perfect Science, 2020 University of Nebraska - Lincoln
Error In Dna Mixture Analysis: Why The Application Of Forensic Dna Is Not A Perfect Science, Rachel Bisbee
Honors Theses
Ever since the first use of DNA evidence in 1986, it has been heavily relied on in court as a means of both convicting felons and exonerating wrongfully imprisoned people. It is a valuable tool in investigations, but like everything else, is not perfect. There have been studies on the accuracy of DNA mixture interpretation in the past with varying results. This is because mixture interpretation is a complicated process that is not always as specific as would be ideal. This study attempted to determine if two students with the exact same education in forensic science and, more specifically, DNA …
Antifungal Defense Molecules From Bacterial Symbionts Of North American Trachymyrmex Ants, 2020 Claremont Colleges
Antifungal Defense Molecules From Bacterial Symbionts Of North American Trachymyrmex Ants, Georgia Scherer
CMC Senior Theses
Defensive symbioses, in which microbes provide molecular defenses for an animal host, hold great potential as untapped sources of therapeutically useful antibiotics. Fungus-growing ants use antifungal defenses from bacterial symbionts to suppress pathogenic fungi in their nests. Preliminary chemical investigations of symbiotic bacteria from this large family of ants have uncovered novel antifungal molecules with therapeutic potential, such as dentigerumycin and selvamicin.
In this study, the bacterial symbionts of North American Trachymyrmex fungus-growing ants are investigated for antifungal molecules. Plate-based bioassays using ecologically-relevant fungal pathogens confirmed that these bacteria have antifungal activity. In order to purify and identify the antifungal …
Environmental Dna Is An Effective Method To Monitor Species In Various Freshwater Habitats, 2020 Central Washington University
Environmental Dna Is An Effective Method To Monitor Species In Various Freshwater Habitats, Kayleigh Mullen
All Master's Theses
This research investigated the use of DNA shed from individuals into the environment (eDNA) to monitor three amphibian species and two trout species associated with habitat intersected by Interstate-90 in Snoqualmie Pass, Washington. This included a large catchment area within creeks and nearby wetlands historically affected by I-90, including sites where significant habitat improvements had been made. Species-specific primers were used to detect three focal amphibians of varying local abundance and two focal trout species. This study showed successful detection of species across both lentic and lotic systems throughout the study area through efficient multiplexing (detection of multiple species in …
Plasticity And The Impact Of Increasing Temperature On A Tropical Ectotherm, 2020 Georgia Southern University
Plasticity And The Impact Of Increasing Temperature On A Tropical Ectotherm, Adam A. Rosso
Electronic Theses and Dissertations
Organisms may respond to climate change through behavior, genetic adaptation, and/or phenotypic plasticity. Tropical ectotherms are thought to be especially vulnerable to climate change because most have a narrow range of thermal tolerance while living close to their upper thermal tolerance limits. Additionally, many tropical species live in closed-canopy forests, which provide homogenous thermal landscapes that prevent behavioral compensation for stressfully warm temperatures. Finally, tropical ectotherms are thought to have decreased capacity for phenotypic plasticity because they have evolved in thermally stable environments. We tested gene expression patterns and phenotypic plasticity in the Panamanian slender anole by a) measuring changes …
A "Choose-Your-Own" Classroom-Based Activity That Promotes Scientific Inquiry About Rna Interference, 2019 Chapman University
A "Choose-Your-Own" Classroom-Based Activity That Promotes Scientific Inquiry About Rna Interference, Jeremy L. Hsu
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
RNA interference (RNAi), the process that results in the degradation of a target gene’s mRNA, is a fundamental part of eukaryotic gene regulation and is also an important molecular technique that allows for experimental manipulation of gene expression without altering DNA sequences. Despite the importance of RNAi, there have been relatively few lecture-based activities designed to teach about the consequences of this process and counter common misconceptions. I present here an inquiry-based activity that is centered around a “choose your own experiment” design where students generate hypotheses and critically evaluate their ideas by choosing several simulated experiments. The activity presents …
Gmr36a08, 2019 University of Dayton
Gmr36a08, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 49927
- ID - GMR36A08
- Location - 2R: 18120673, 18124576
- Base pairs - 3903
Gmr40e08, 2019 University of Dayton
Gmr40e08, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 41238
- ID - GMR40E08
- Location - 2R: 18162090, 18165058
- Base pairs - 2968
Gmr42a07, 2019 University of Dayton
Gmr42a07, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 45284
- ID - GMR42A07
- Location - 2R: 18146520, 18150306
- Base pairs - 3786
Gmr36a10, 2019 University of Dayton
Gmr36a10, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 49373
- ID - GMR36A10
- Location - 2R: 18116788, 18120701
- Base pairs - 3913
Gmr40c09, 2019 University of Dayton
Gmr40c09, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer
- ID - GMR40C09
- Location - 2R: 18132203, 18134987
- Base pairs - 2784
Gmr41f05, 2019 University of Dayton
Gmr41f05, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 50133
- ID - GMR41F05
- Location - 2R: 18139042, 18142672
- Base pairs - 3630
Gmr35a04, 2019 University of Dayton
Gmr35a04, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer
- ID - GMR35A04
- Location - 2R: 18125755, 18128365
- Base pairs - 2610
Gmr39h01, 2019 University of Dayton
Gmr39h01, Anuradha Venkatakrishnan Chimata, Oorvashi Roy Puli, Amit Singh
DVE Enhancer Sequences Utilized in GMR Line
dve enhancer 50066
- ID - GMR39H01
- Location - 2R: 18153797, 18156108
- Base pairs - 2311