Food Science Commons^™

Detection Of Food Proteins In Human Serum Using Mass Spectrometry Methods, Abigail S. Burrows

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Allergenic peanut proteins are highly resistant to digestion and are detectable by immunoassays after gastrointestinal digestion. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for in vivo detection of peptides originating from allergenic food proteins has not been thoroughly studied. The aim of this work was to develop an in vivo detection method for peanut proteins in serum using LC-MS/MS. The method(s) were validated by analyzing subject serum collected after peanut consumption.

Three de-complexation strategies were evaluated including (1) MS acquisition settings (i.e. inclusion, exclusion lists), (2) commercial depletion kits, and (3) organic solvent fractionation by discovery LC-MS/MS. Overall, …

In Vitro Anti-Oxidation, Anti-Hepatic Steatotic And Anti-Inflammatory Effects Of Auricularia Nigricans (Syn A. Polytricha), Hoe Leong Teoh

Student Works (2020-2029)

At present, non-alcoholic fatty liver disease (NAFLD) is one of the most frequent liver diseases worldwide. In general, NAFLD is asymptomatic, and can slowly progress to serious complications such as cirrhosis, hepatocellular carcinoma, cardiovascular disease and death. Although there are no approved treatment regimens for NAFLD at this moment, lifestyle modifications through diet is the mainstay of treatment. Auricularia nigricans is a popular edible mushroom known to possess medicinal properties such as antioxidant, antitumor and anti-inflammatory. The present study was conducted to determine the beneficial effects of A. nigricans against NAFLD through investigation of the in vitro free radical scavenging …

In Ovo Administration Of Defined Lactic Acid Bacteria Previously Isolated From Adult Hens Induced Variations In The Cecae Microbiota Structure And Enterobacteriaceae Colonization On A Virulent Escherichia Coli Horizontal Infection Model In Broiler Chickens, Margarita A. Arreguin-Nava, Brittany D. Graham, Bishnu Adhikari, Melissa Agnello, Callie M. Selby, Xochitl Hernandez-Velasco, Christine N. Vuong, Bruno Solis-Cruz, Daniel Hernandez-Patlan, Juan David Latorre, Guillermo Tellez Jr., Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The effects of in ovo administration of a defined lactic acid microbiota (LAM), previously isolated from adult hens, in the cecae microbiota structure and Enterobacteriaceae colonization after exposure to virulent Escherichia coli during the hatching phase of broiler chickens were evaluated. Embryos inoculated with LAM showed a significant (P < 0.05) reduction of Enterobacteriaceae colonization at day-of-hatch (DOH) and day (d) 7. Furthermore, there was a significant increase in total lactic acid bacteria on DOH, body weight (BW) DOH, BW d7, and d0–d7 BW gain and reduced mortality d0–d7 was observed in the LAM group compared with that in phosphate-buffered saline (PBS) control. …

Effect Of Long Pasteurization Run Times On Bacterial Numbers In Milk, Brynli Tattersall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the Western Dairy Center to understand how long a milk pasteurizer can be operated before increases in bacterial numbers are observed in the pasteurized milk. While pasteurization kills pathogenic bacteria there are some non-pathogenic bacteria that can survive and have the ability to become attached to the surfaces in the cooling sections of the pasteurizer. Some bacteria can also produce spores that survive pasteurization even if the bacterial cells are killed. Temperatures in the cooling section remain in a range suitable for growth of these heat-tolerant bacteria and can allow germination of bacterial spores. While …

Reusable Light-Activated Antimicrobial Materials Prepared With Food Ingredients, Andrew T. Gagon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Microbial contamination and proliferation in food processing, distribution and storage continues to be a real risk in spite the wide variety of food processing and preservation techniques available. Some of these techniques (such as heat treatments and the use of chemical preservatives) may require substantial amounts of energy and their effects in the general population and the environment may be harmful. This has generated an interest over alternative methods of food preservation. The use of reusable light-activated materials may represent an effective approach. In this study, a polypropylene plastic was modified with either zein, which is found in corn, or …

Exploring The Connection Between Acid Exposure And Virulence In Listeria Monocytogenes, Minghao Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Listeria monocytogenes is a gram-positive food-borne pathogen that is widely dispersed in the environment and can cause listeriosis with high fatality rates when consumed in contaminated food products. They are capable of growing over a wide range condition. Listeria is also able to tolerate adverse conditions which allows the bacterium to survive in unfavorable environments. The ubiquitous nature of L. monocytogenes makes it difficult to eliminate from food systems. One major problem in the food industry is the survival of L. monocytogenes under sublethal low pH-environment since organic acids are widely used as food decontaminants. Prior research has suggested that …

Consumer Attitudes, Knowledge, And Behavior: Understanding Gluten Avoidance And Point-Of-Decision Prompts To Increase Fiber Consumption, Kristina Arslain

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Understanding consumer food behavior is important for creating effective health programs and policies that address obesity. In this thesis, two topics of consumer food behavior are explored: understanding gluten avoidance and the use of point-of-decision prompts (PDP) to increase the purchase of healthier food choices. In this first topic, nationally representative data was collected on people’s gluten-free (GF) experience and perceptions. It was found that people were more likely to avoid gluten if they believed the (GF) diet was healthier than a gluten-containing diet and if they were recommended to try the diet. The second topic studies the influence of …

Effects Of Different Separation Techniques On The Component Content Of Linear Maltooligosaccharides, Li Jia-Cheng, Chen Dian-Ning, Gu Zheng-Biao, Li Cai-Ming, Li Zhao-Feng

Food and Machinery

In order to improve the content of linear maltooligosaccharides with specific degree of polymerization in the enzymatic hydrolysis products, the effects were investigated including from the membrane separation, activated carbon adsorption separation, sephadex column chromatography and ion exchange resin column chromatography. The results showed that the membrane separation, activated carbon adsorption separation, sephadex column chromatography and Na⁺/Ca²⁺ ion exchange resins were all not conducive to continuous batch operation. In contrast, K+ ion exchange resins had better effects. Compared with the initial enzymatic hydrolysate, the proportion of G3~G6 and G5+G6 in the permeable solution after separation and purification …

Quality Differences And Correlation Analysis Of Natto Fermented From Soybean Seeds With Different Protein Content, Lin Yong-Shan, Gao Xiang-Yang, Cheng Jia, Zeng Jian, Chen Rui-Zhi, Liu Yu-Long, Yang Cun-Yi

Food and Machinery

Seven genotype soybean seeds with different protein content were using as the raw material for natto fermentation. The study was focus on the difference of the sensory quality and properties of mucilage on the surface of natto after fermented by Bacillus subtilis natto. Then the relationship between the quality of mucilage and the quality traits of soybean seeds was analyzed. The results showed that, the sensory score and stretch length of natto mucilage fermented from five different genotype soybean except green soybean YSG-10 and black soybean YSB-37 were significantly negatively correlated with seeds protein content respectively (r are 0.953, 0.946 …

Identification And Polar Lipid Composition Analysis Of Yeasts Isolated From Sufu Manufacture, Chen Si-Kai, Lin Sheng-Jie, Li Li

Food and Machinery

In order to characterize lipid metabolism of yeasts during sufu manufacture, 23 isolates from sufu pehtze and acidic tofu whey were identified. Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to analyse the lipid fingerprints of 7 strains of yeast, after the determination of lipase activities. It is suggested that lipid fingerprint mainly includes 2 kinds of sphingosine, 16 kinds of amide, 22 kinds of ester and 3 kinds of fatty acid. Kluyveromyces marxianus SP-1 and Candida (Pichia) norvegensis SP-5 show highest relative amount of 2-Amino-1,3,4-octadecanetrio and N, N-Dimethylsphingosine, which indicates potential for sphingolipids synthesis. Candida ethanolica ATW-1, Pichia …

Dynamic Analysis Of Lf-Nmr Relaxation Signals And Quality Characteristics During Heating Beef, Xie An-Guo, Wang Man-Sheng, Shi Xiao-Wei, Wang Fei-Xiang, Kang Huai-Bin

Food and Machinery

Through intensive sampling of beef during the heating process, the changes of meat quality and all parameters of relaxation signal peaks were studied. The results showed that with the increase of heating time, the semi combined water decreased while the free water increased. In terms of peak shape characteristics, T₂₁ peak width data fluctuated more, T₂₂ peak width increased and height decreased, and the positions of the three peaks shifted left significantly, which reflected that the ordered spatial structure of protein was destroyed and a large number of disordered curls were formed after heat denaturation. The average distance …

Effect Of Natural Polysaccharide On Moisture Retention Capacity And Moisture Kinetic Of Tobacco, Lei Sheng, Liu Xiu-Ming, Jiang Ju-Xing, Shao Shuai-Zhen, Zhou Xue, Zhang Xiao-Ming, Yang Qian-Xu

Food and Machinery

Thermodynamic moisture retention properties of microbial polysaccharides and seaweed polysaccharides were investigated as compared with water and propylene glycol. Then three common dynamic models of tobacco were selected to fit the hygroscopic and desorption processes of tobacco. The results showed that the moisture retention of Laminaria japonica polysaccharides and Spirulina platensis polysaccharides was significantly better than that of propylene glycol, and page model was the best. Considering the thermodynamic and kinetic factors according to the moisturizing index, Laminaria japonica, Spirulina platensis and Hericium erinaceus polysaccharides were considered to have better moisturizing effect. Low field nuclear magnetic results showed that the …

Effect Of Processing Parameters On The Structure Of Soybean Polysaccharide And Its Foaming Properties, Zhang Quan, Chen Jie, Zeng Mao-Mao, He Zhi-Yong

Food and Machinery

Soluble soy polysaccharides (SSPS) with different structure characteristics were obtained by controlling the conditions of acid hydrolysis, alkaline de-esterification and proteolysis. The effect of processing on the structure characteristics of SSPS and its foaming properties were discussed in details. The structure properties of SSPS were characterized by monosaccharide composition, average molecular weight, degree of esterification (DE), and protein content. The foaming properties of SSPS were expressed by the foam expansion rate (FE) and the foam stability (FS). The results showed that the acidic hydrolysis process could degrade the SSPS main chain structure and gradually reduce the molecular weight pf SSPS, …

Analysis Of Puff-By-Puff Release Of Nine Main Components In Mainstream Smoke Of Typical Flue-Cured Slim And Normal Cigarettes, Liu Qi, Ma Meng-Jie, Gong Zhen-Lin, Yuan Yi-Lai, Zhu Huai-Yuan, Wu Yang, Liao Hui-Yun

Food and Machinery

In order to investigate the difference of the puff-by-puff release of nine major components (tar, nicotine, CO, NNK, B[a]P, phenol, crotonaldehyde, HCN and NH3) in mainstream smoke of slim and normal cigarettes, typical flue-cured cigarette samples from same cut tobacco were smoked by smoking machine under ISO. Results showed that: ① The average puffs of slim and normal cigarettes were 5.49 and 6.72 respectively, which showed there were significant statistical differences. In general, the puff-by-puff and total release of all components from slim cigarette were lower than from normal cigarette. and puff-by-puff release deviations of tar, nicotine, NNK, B[a]P, phenol, …

Analysis And Evaluation Of Nutrient Compositions Of Miichthys Miiuy Muscle And By-Products, Sun Su-Ling, Li Xue, Gu Xiao-Hong, Zhang Yu, Wang Jun-Hong, Zhu Zuo-Yi, Wang Wei

Food and Machinery

To determine the nutrients contents in Miichthys miiuy muscle and by-products. The contents of moisture in Miichthys miiuy muscle and by-products were 79.65%, 69.15%; crude protein were 18.65%, 19.36%; crude fat were 0.24%, 3.90%; and crude ash were 1.15%, 3.75%, respectively. Total amino acids (TAA) contents of Miichthys miiuy muscle and by-products were 18.58%, 15.47%, and essential amino acids (EAA) contents were 7.65%, 5.52%, respectively. However, only EAA/TAA (41.17%) and EAA/non essential amino acids (NEAA, 69.99%) of Miichthys miiuy muscle met FAO)/WHO standard for protein profile. According to amino acid score (AAS) and chemical score (CS), methionine was the first …

Study On The Quality Of Raw And Cooked Chestnut From Different Growing Regions, Zhou Kui, Zhang Ya-Yuan, Huang Hui-Ling, You Xiang-Rong, Wang Ying, Li Ming-Juan, Wei Ping

Food and Machinery

In order to understand the comprehensive nutritional value of Guangxi chestnut growing in Counties Tiane, Longlin and Longan, the nutritional components, antioxidant components and processing characteristics of raw and cooked chestnut were compared and analyzed. The results showed that the quality of chestnut varies with the place of production and the state of ripeness. The cooked chestnut had a darker color, higher fat, amino acid content and water holding capacity, while the raw chestnut had higher soluble protein and vitamin C (V_C) content, and stronger ability of scavenging free-radical and oil-holding capacity. However, the raw chestnut exhibited the …

Detection Of Enrofloxacin Residues In Chicken Based On Surface Enhanced Raman Spectroscopy And Two-Dimensional Correlation Spectroscopy, Ban Jing-Jing, Liu Gui-Shan, He Jian-Guo, Cheng Li-Juan, Fan Nai-Yun, Yuan Rui-Rui

Food and Machinery

The surface-enhanced Raman spectroscopy (SERS) technology and the two-dimensional correlation spectroscopy (2D-COS) were used to optimize the characteristic variables of the Enrofloxacin in chicken meat. The partial least squares regression method (PLSR) was used to establish Enro the characteristic peak analysis model of sand star was compared with the competitive positive adaptive weighting algorithm (CARS). The results showed that the 2D-COS-PLSR model has the best effect, and its Rc and Rp were 0.979 7, 0.997 2 respectively, which shows that it is feasible to use 2D-COS to optimize the characteristic spectral peaks related to the concentration of enrofloxacin in chicken.

Establishment Of Double Antibody Sandwich Elisa For Peanut Allergenic Protein Ara H 1, Wang Yao, Chen Xi, Wu Han-Liang, Wei Xing, Zhang Guo-Qing, Zhang Shu-Xia, Liu Sheng-Nan

Food and Machinery

In order to establish a double antibody sandwich ELISA method for the rapid, sensitive and specific detection of peanut allergenic protein Ara h 1. Taking Ara h 1 murine monoclonal antibody (mAb) as capture antibody, and Ara h 1 rabbit polyclonal antibody (pAb) as detection antibody, the checkerboard method was used to optimize the antibody working concentration to establish the method, and the detection characteristics were identified, including sensitivity, specificity, accuracy and stability of the method. Results: The optimal working concentrations of mAb and pAb were 1∶10 000 and 1∶8 000 dilution, respectively. The linear range of the ELISA standard …

Quickly Determination Of Bromate And Nitrite In Drinking Water By High Pressure Ion Chromatography, Chen Jing-Zhou, Li Liang, Huang Shao-Man, He Shuang

Food and Machinery

A method of high pressure ion chromatography (HPIC) for quickly determinating bromate and nitrite in drinking water was established. The contents of bromate and nitrite in the sample were determined by using Dionex Integrion high-pressure ion chromatograph, IonPac AS19 high-capacity analytical column and inhibition conductivity detector, with the eluent of KOH produced on-line by automatic generation device. The anions of bromate and nitrite could be separated by the method within 5 min with detection limits of 0.46 μg/L and 0.22 μg/L respectively. In the concentration range of 0~100 μg/L for bromate and 0~50 μg/L for nitrite, a good linear relationship …

Simultaneous Determination Of 10 Kinds Of Fungicides In Tomatoes And Cucumbers By Gc-Ms/Ms Combined With Solid Phase Extraction, Zhang Yao, Wu Long-Guo, Ma Gui-Juan, Gong Hui, Gao Lin, Sun Qian

Food and Machinery

A method for the simultaneous determination of 10 fungicides residues in vegetables by gas chromatography- mass spectrometer (GC-MS/MS) was established. The samples were extracted by ethyl acetate cyclohexane, and the aminosolid phase extraction column purification was preferred. Which were detected by multi-reaction monitoring (MRM), and quantified by external standard method. The results showed that the linear relationship of 10 fungicides in the mass concentration range of 15~500 ng/mL was good, and the correlation coefficient was all greater than 0.999 1, with the detection limit of 0.1~0.5 μg/kg. The actual recoveries of the samples were 84.7%~117.0%, and the relative standard deviation …