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Effect Of Three Reducing Sugars On Emulsifying Property And Structure Of Maillard Products Of Kidney Bean Protein, LIN Wei, LIU Xiaolan, REN Jian, GAO Jian, DU Hong 2019 College of Food and Bioengineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China; Key Laboratory of Processing Agricultural Products, Qiqihar, Heilongjiang 161006, China

Effect Of Three Reducing Sugars On Emulsifying Property And Structure Of Maillard Products Of Kidney Bean Protein, Lin Wei, Liu Xiaolan, Ren Jian, Gao Jian, Du Hong

Food and Machinery

The purpose of this study was to investigate the effect of sugars on the structural and emulsifying property of kidney bean protein modified by Maillard reaction. The results showed that the emulsifying activity and stability of kidney bean protein could be improved by Maillard reaction with glucose, fructose and lactose, and fructose was the best one. The emulsifying ability increased from 37.52 m2/g to 98.69 m2/g, and the emulsifying stability also increased from 16.88 min to 28.68 min; After Maillard reaction, the spatial structure of protein changed, which showed that the surface hydrophobicity increased, the content …


Optimization On Osborne Extracting Process For Mung Bean Albumin And Its Subunit Strip Determination, ZHANG Shu, WANG Changyuan, TAN Zhaoyin, FENG Yuchao 2019 Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China;National Cereals Engineering Technology Research Center, Daqing, Heilongjiang 163319, China

Optimization On Osborne Extracting Process For Mung Bean Albumin And Its Subunit Strip Determination, Zhang Shu, Wang Changyuan, Tan Zhaoyin, Feng Yuchao

Food and Machinery

Taking the mung beans as raw materials, the Osborne classification method was used to extract albumin from mung bean. Based on the single factor test, using the L9 (34) orthogonal test, the effects were studied, from the extraction temperature, extraction time and ratio of material to liquid on the extraction rate of mung bean albumin. The subunit composition of mung bean albumin was analyzed by SDS-PAGE. The results showed that the optimal extraction process of mung bean albumin were: ratio of solid to liquid of 1∶10 (g/mL), extraction temperature of 45 ℃, extraction time of 150 min. …


Research On Production Process And Product Characteristics Ofnutritional Fermented Milk In Chickpea, CHEN Xiajing, LIU Hongyu, LI Yanze, WANG Ying, WANG Changyuan, YAO Di 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Research On Production Process And Product Characteristics Ofnutritional Fermented Milk In Chickpea, Chen Xiajing, Liu Hongyu, Li Yanze, Wang Ying, Wang Changyuan, Yao Di

Food and Machinery

Chickpeas and milk were used as raw materials, and the chickpea fermented milk was prepared by mixed strains of SL and LSB. The single factor and orthogonal experiment were used, and the processing conditions of chickpea fermented milk were optimized with sensory score. At the same time, the physical and chemical properties, rheological properties and nutritional composition of the product were determined, the shelf life of the product was determined by microbial index and sensory evaluation. The results showed that optimal conditions of fermented milk of SL strains were the immersing chickpeas time of 5 h, the inoculum doses of …


Optimization Of Extraction Process Of Mung Bean Polyphenols And Antioxidant Properties, SHENG Yanan, WANG Changyuan, ZHANG Shu, FU Tianxin, FENG Yuchao, QIN Haibo 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization Of Extraction Process Of Mung Bean Polyphenols And Antioxidant Properties, Sheng Yanan, Wang Changyuan, Zhang Shu, Fu Tianxin, Feng Yuchao, Qin Haibo

Food and Machinery

Response surface method (RSM) was used to optimize the extraction process of mung bean polyphenols and study its antioxidant activity. The results showed that the optimal extraction process was 1∶12 (g/mL), extracted at 46 ℃ for 1.6 h. The amount of polyphenol extracted under the control of these conditions was 11.74 mg/g. The rate of polyphenol superoxide anion free radical scavenging in mung bean was 58.5%, and that of the nitrite ion clearance was 26.0%. Therefore, it inclined that the polyphenols from mung bean had good antioxidant activity.


Effects Of Three Drying Methods On The Dryingcharacteristics And Quality Of Buckwheat, ZHANG Yiwen, ZHANG Yanliang, WAN Lin, CHE Gang, GAO Ruili, WANG Hongchao 2019 College of Engineering, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Effects Of Three Drying Methods On The Dryingcharacteristics And Quality Of Buckwheat, Zhang Yiwen, Zhang Yanliang, Wan Lin, Che Gang, Gao Ruili, Wang Hongchao

Food and Machinery

The buckwheat was dried by vibrating far infrared drying, drying in oven and drying in thin layer, and compared the drying characteristics and quality after drying to the buckwheat by the three kinds of drying methods. Result: The buckwheat had the shortest safe water time and the highest drying rate by vibrating far infrared drying. The drying time of thin layer was the longest and the drying rate was the lowest. In terms of quality analysis, vibrating far-infrared drying process had little effect on the appearance quality of buckwheat. The area shrinkage rate W of dried buckwheat was the smallest, …


Optimization Of Ultrasonic Assisted Enzymatic Extraction Of Dietary Fiber From Light Speckled Kidney Bean, ZHANGN Yanli, WANG Ying, WANG Di, ZUO Zhaohang, LIU Shuting 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization Of Ultrasonic Assisted Enzymatic Extraction Of Dietary Fiber From Light Speckled Kidney Bean, Zhangn Yanli, Wang Ying, Wang Di, Zuo Zhaohang, Liu Shuting

Food and Machinery

Ultrasonic assisted complex enzyme (1.0% alkaline protease and 0.2% high temperature resistant alpha-amylase) was used to extract dietary fiber from milk white colored kidney bean residue after enzymolysis.The effect of ultrasonic conditions on the extraction rate of water-insoluble dietary fiber (IDF) and water-soluble dietary fiber (SDF) was studied.The results showed that the extraction rate of IDF and SDF reached 60.11% and 5.63% respectively, when extracted with 250 W of ultrasonic at 60 ℃ 25 min. There were characteristic absorption peaks in the infrared spectra of DF. The water holding capacity of SDF was 1.828 g/g higher than that of IDF, …


Optimization On Fermentation Technology Of Kidney Bean Enzyme, WANG Di, WANG Ying, ZHANGN Yanli, LIU Shuting, ZUO Zhaohang 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization On Fermentation Technology Of Kidney Bean Enzyme, Wang Di, Wang Ying, Zhangn Yanli, Liu Shuting, Zuo Zhaohang

Food and Machinery

Lactic acid bacteria and yeast were used to prepare enzymes, and the single factor experiment of multiple strains was optimized to determine the inoculation sequence and fermentation time of the complex fermentation. Results: the optimal inoculation order was inoculated yeast first, and then lactic acid bacteria. The optimal compound fermentation condition was: inoculated with 0.20% yeast under the condition of bottling volume of 30 mL(250 mL) and pH 5.0, and shaking incubated at 30 ℃ for 24 h. Then 3.0% lactobacillus complex was inoculated (lactobacillus plantarum to lactobacillus acidophilus was 1∶1) and cultured at 37 ℃ for 24 h with …


Comparing Of Two Seperation Processes For Recovering Xylose Mother Liquor With Sequential Simulated Moving Bed Technology, LI Hongfei, SUN Daqing, LI Liangyu, YU Wei, ZHANG Liping 2019 National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Comparing Of Two Seperation Processes For Recovering Xylose Mother Liquor With Sequential Simulated Moving Bed Technology, Li Hongfei, Sun Daqing, Li Liangyu, Yu Wei, Zhang Liping

Food and Machinery

The two kinds of sepatation process for the xylose mother liquor of xylose, arabia sugar and glucose was studied which used the sequential simulated moving bed (SSMB) chromatographic separation equipment. In the optimal separation conditions of the separation process for two components, the purity of xylose and arabia sugar reached 86.30% and 88.41%, and recovery rate reached 85.20% and 89.26%. During the separation process of the three components, the purity of xylose and arabia sugar reached 77.73% and 79.94%, and the recovery rate reached 82.00% and 87.00%, respectively. Xylose mother liquor recovery processing was very difficult, the problem can be …


Optimization Of Xylanase Production By A Neurospora Sitophilathrough Solid State Fermentation, DUAN Rui, DENG Yongping, LIU Xiaolan, ZHENG Xiqun, JIANG Huanxiao, MA Rui 2019 College of Food and Biotechnology, Qiqihar University, Qiqihar, Heilongjiang 161006, China

Optimization Of Xylanase Production By A Neurospora Sitophilathrough Solid State Fermentation, Duan Rui, Deng Yongping, Liu Xiaolan, Zheng Xiqun, Jiang Huanxiao, Ma Rui

Food and Machinery

Using vinegar as the main raw material to produce xylanase by solid-state fermentation of Neurospora sitophila. Using the activities of xylanase as indicators, the medium and culture conditions were optimized by single factor experiment and orthogonal experiment. Results: The medium consisted of 5 g of vinegar and 0.4 g of bean dregs. The initial pH of the medium was 5.5, 106 spores/Bottle of inoculum concentration and the solid-liquid ratio was 1∶3 (g/mL), and the culture medium was cultured at 28 ℃ for 3 d. The enzyme activity was 412.34 U/g under the optimized conditions, which was increased about 3.59 times …


Effects Of Glycosylation Modification On The Antioxidant Activity Of Casein-Enzymatic Hydrolysate, ZHAO Yubin, MU Qiuxia, QU Liuqing, CUI Supin 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Effects Of Glycosylation Modification On The Antioxidant Activity Of Casein-Enzymatic Hydrolysate, Zhao Yubin, Mu Qiuxia, Qu Liuqing, Cui Supin

Food and Machinery

In order to enhance the antioxidant activity of the casein hydrolysate, the enzymatic hydrolysate was modified by glycosylation with glucose, xylose and fructose, respectively. The effects of modification conditions on the antioxidant activity of the product were investigated under the mass ratio of enzymatic hydrolysate to sugar 1∶2. The results showed that the hydroxyl radical clearance rate reached the maximum at pH 7.0, which were 38.63% and 32.82%, respectively, and each was higher than the control. At pH 7.0, the reducing ability of the enzymatic hydrolysate was the strongest when modified by glycosylation with glucose or xylose. As the reaction …


Aerobic Acclimation Of Bifidobacterium Adolescentis And The Effect On Its Proliferation Of Different Oligosaccharides, LI Yanze, CHEN Xiajing, ZHANG Shiang, JIN Xin, XU Lei, YAO Di 2019 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Aerobic Acclimation Of Bifidobacterium Adolescentis And The Effect On Its Proliferation Of Different Oligosaccharides, Li Yanze, Chen Xiajing, Zhang Shiang, Jin Xin, Xu Lei, Yao Di

Food and Machinery

In order to improve the situation on low number of living bacterium and the weak strain effect on Bifidobacterium adolescentis in fermented products, the anaerobic B. adolescentis was oxygen-resistant domesticated, by using anaerobic and aerobic alternating domesticated method, and the partial pressure of oxygen in the culture medium of B. adolescentis was gradually increased in the process of acclimation. The physiological characteristics before and after the domestication of B. adolescentis was determinated and the effects on its proliferation of the soybean oligosaccharides, xylooligosaccharide and oligomeric maltose were compared. The results showed that the sensitive degree to oxygen in B. adolescentis …


Optimization Of Extruded Process For Bean-Based Multigrain Rice Paste, LIU Shuting, WANG Ying, SHEN Yan, SUN Yueru, ZUO Zhaohang 2019 College of Food, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization Of Extruded Process For Bean-Based Multigrain Rice Paste, Liu Shuting, Wang Ying, Shen Yan, Sun Yueru, Zuo Zhaohang

Food and Machinery

Taking bean dregs as the main raw material, compounding miscellaneous grains and homology of medicine and food to prepare soybean-based multigrain rice paste. The material addition amount, screw speed, and the temperature of the zone III barrel were used as response factors, and the gelatinization degree was the investigation index. On the basis of the single factor experiment, the optimal process conditions for the preparation of bean-based multigrain rice paste by the extrusion-expansion technique were obtained by Design-Expert 8.0.6 design test. The results showed that when the material addition amount was 12.5%, the screw rotation speed was 330 r/min, and …


Genetic Diversity Analysis Of Common Bean Germplasms Based On Ssr Molecular Markers, XIA Chunyang, DU Jidao, HAN Yiqiang, SUN Haoyue, LI Ming, WU Hongbin, ZHANG Qi, YU Shenglong 2019 College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Genetic Diversity Analysis Of Common Bean Germplasms Based On Ssr Molecular Markers, Xia Chunyang, Du Jidao, Han Yiqiang, Sun Haoyue, Li Ming, Wu Hongbin, Zhang Qi, Yu Shenglong

Food and Machinery

The genetic diversity of 69 common bean germplasm resources was analyzed by SSR-PCR reaction system, and cluster analysis was carried out by UPGMA method. Seventeen pairs of SSR markers were detected and 55 allelic mutation loci were obtained, ranging from 2 to 5, with an average of 3.24 markers per pair. Seventeen pairs of primers had 17 polymorphic loci with 100% polymorphism. According to the cluster analysis results, the genetic similarity coefficient (GS) of the material ranged from 0.392 to 0.963, with an average of 0.659. 69 kinds of the beans seeds were divided into four groups at the genetic …


Functional Properties And Subunits Properties Of Oat Protein Isolate Under Different Ph And Ionic Strength, WANG Meiyu, WANG Yu, CHEN Zhenjia, YAN Zhou, LIU Longlong 2019 Shanxi Agricultural University, Jinzhong, Shanxi 030801, China

Functional Properties And Subunits Properties Of Oat Protein Isolate Under Different Ph And Ionic Strength, Wang Meiyu, Wang Yu, Chen Zhenjia, Yan Zhou, Liu Longlong

Food and Machinery

Oat protein isolate (OPI) was extracted from oat. The solubility, foaming properties, emulsifying activity and subunits properties of OPI under different pH and ionic strength were studied. The results showed that,at pH 5.0~6.0, OPI has the lowest solubility, the lowest foaming, the highest foam stability, and under alkaline conditions higher emulsification and emulsion stability. Acidic conditions of pH 2.0 and 3.0 cause partial hydrolysis of soluble OPI peptide chains, forming a subunit between 31.0 to 43.0 kDa. No significant difference was found under the alkaline conditions. The solubility was the lowest when the NaCl concentration was 0.05 mol/L, and The …


Improved Design And Test Of A Small Twin-Screw Press In Low Temperature, CAO Meili, ZHANG Yonglin, LI Shilong, GAN Weirui 2019 Wuhan Polytechnic University, Wuhan, Hubei 430023, China

Improved Design And Test Of A Small Twin-Screw Press In Low Temperature, Cao Meili, Zhang Yonglin, Li Shilong, Gan Weirui

Food and Machinery

The oil extracted by the low temperature twin-screw press is healthy. The structure of the low temperature twin-screw press is big and complicated, so it is not suitable for the rural market. In order to improve the quality of the oil for the peasants, a new small low temperature twin-screw press is improved and designed based on the large and media presses, which was composed of the srews, pressing and shearing rings. The shearing rings were new elements, which could shear the oilseed, increase the pressure and speed up the extrusion of the oil. The number and location of the …


Study On Antithrombotic Effect And Mechanism Of Lemon Juice Powder, XU Congyue, LIN Kuan, LI Chaoying, RU Qin 2019 Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, Hubei 430056, China

Study On Antithrombotic Effect And Mechanism Of Lemon Juice Powder, Xu Congyue, Lin Kuan, Li Chaoying, Ru Qin

Food and Machinery

The acute blood stasis rat model was made by injecting norepinephrine, then rats stayed in ice water bath for 4 mins. Whole blood viscosity (WBV), erythrocyte sedimentation rate(ESR), coagulation parameters, 6-keto prostaglandin F1α (6-KETO-PGF1α), thromboxane B2 (TXB2), endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) were measured. Results: Model group significantly up WBV and ESR level, APTT, PT, TT were decreased whereas FIB content was increased very significantly in rats of the model group, meanwhile the 6-keto-PGF1α and eNOS level were lower and TXB2 and ET-1 level were higher. Lemon juice powder groups might down WBV …


Protective Effects Of Alcohol Extraction From Peony Stamen On H2O2-Induced Damage In Huvec, LUO Lei, GUAN Ningning, XIANG Jinle, ZHU Wenxue 2019 College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; Henan Agricultural Products Drying Equipment Engineering Technology Research Center, Luoyang, Henan 471023, China

Protective Effects Of Alcohol Extraction From Peony Stamen On H2O2-Induced Damage In Huvec, Luo Lei, Guan Ningning, Xiang Jinle, Zhu Wenxue

Food and Machinery

The main components of the alcohol extraction from peony stamen was analyzed by high performance liquid chromatography. H2O2 induced human umbilical vein endothelial cells (HUVEC) cells to establish a damage model to study the protective effect of peony flower extract. Results: the alcohol extraction from peony stamen mainly contain three monomers, namely rutin, quercetin and paeoniflorin, with contents of 44.25%, 15.50% and 17.00%, respectively. The alcohol extraction from peony stamen can reduce the content of MDA in cells and culture medium, increase the activities of SOD, GPX and GSH content in the calls. The alcohol extraction of …


Optimization On Pre-Fermentation Process Of Black Bean Douchi Fermented By Bacillus Subtilis Natto, ZHANG Qi, ZHU Dan, NIU Guangcai, WEI Wenyi, ZHAO Jing, WU Yue 2019 Food College, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization On Pre-Fermentation Process Of Black Bean Douchi Fermented By Bacillus Subtilis Natto, Zhang Qi, Zhu Dan, Niu Guangcai, Wei Wenyi, Zhao Jing, Wu Yue

Food and Machinery

Taking the black bean as the main raw material, the total polyphenol content and DPPH radical scavenging rate as the indexes, the single factor experiment and orthogonal experiment design were used to optimize the pre-fermentation conditions of black bean douchi made by bacillus subtilis natto. The results showed that the optimum fermentation conditions were as follows: fermentation time was 4 d, fermentation temperature was 39 ℃, inoculation amount of bacillus subtilis natto was 2.5 g/100 g·black bean. Under this condition the total polyphenol content of black bean douchi was 4.24 mg/g, and the DPPH radical scavenging rate was 79.86%, which …


Study On Antioxidant Activities Of Various Solvent Polyphenol Extracts From Black Soybean Seed Coat, REN Manni, WANG Cuntang, TANG Xuhua, SUN Peng, GAO Zengming 2019 College of Food and Biological Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China

Study On Antioxidant Activities Of Various Solvent Polyphenol Extracts From Black Soybean Seed Coat, Ren Manni, Wang Cuntang, Tang Xuhua, Sun Peng, Gao Zengming

Food and Machinery

Black soybean seed coat were extracted using three solvent systems: 70% acetone, ethanol and methanol. The extracts were tested for their total phenolic content, total flavonoid content and antioxidant activities. The results showed that the extracts with acetone as the extraction agent had the highest yield[(11.23±0.005) g/100 g], and the extracts had the highest total phenol content of (845.32±21.35) mg/g and total flavonoids content of (63.72±2.35) mg/g. Meanwhile, IC50 of acetone extract on DPPH free radical and ABTS free radical were (0.059±0.005) mg/mL and (0.057±0.004) mg/mL, respectively. The antioxidant activities were lower than ascorbic acid but higher than methanol …


Effects Of Poor Sanitation Procedures On Cross-Contamination Of Animal Species In Ground Meat Products, Sunjung Chung, Rosalee S. Hellberg 2019 Chapman University

Effects Of Poor Sanitation Procedures On Cross-Contamination Of Animal Species In Ground Meat Products, Sunjung Chung, Rosalee S. Hellberg

Food Science Faculty Articles and Research

The presence of <1% of an undeclared species in ground meat is generally thought to be indicative of cross-contamination as opposed to intentional mislabeling; however, this has not been experimentally tested. The objective of this study was to quantify the effects of poor sanitation on the cross-contamination of animal species in ground meat products, with the example of undeclared pork in ground beef. Cross-contamination was quantified using real-time polymerase chain reaction (PCR). Three different sanitation treatments were tested with a commercial grinder (“no cleaning”, “partial cleaning”, or “complete cleaning”) in between grinding of pork and beef samples (13.6 kg each). A 100-g sample was collected for each 0.91 kg (2 lb) of beef processed with the grinder and each sanitation treatment was tested twice. For the “no cleaning” treatment, the first 100-g sample of ground beef run through the grinder contained 24.42 ± 10.41% pork, while subsequent samples (n = 14) contained <0.2% pork. With “partial cleaning,” the first sample of ground beef contained 4.60 ± 0.3% pork and subsequent samples contained <0.2% pork. Pork was not detected in ground beef following “complete cleaning.” These results indicate that incomplete cleaning of grinding equipment leads to species cross-contamination at levels of <1% in most cases. Proper sanitation procedures must be followed when grinding multiple species in order to prevent cross-contamination and product mislabeling.


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