Establishment Of Multiplex Real-Time Pcr Assays Based On Hand System For The Preliminary Screening Of Five Diarrheagenic Escherichia Coli,
2019
[JP3]Hebei University of Engineering, College of Life Sciences and Food Engineering, Handan, Hebei 056038, China
Establishment Of Multiplex Real-Time Pcr Assays Based On Hand System For The Preliminary Screening Of Five Diarrheagenic Escherichia Coli, Zhong Yike, Wang Yongxia, Zhao Tong, He Xiaoming, Guo Jianshu, Liu Wei, Zou Dayang
Food and Machinery
The specificity of this method was evaluated by testing 17 species closely related bacteria in the laboratory, and the amplification product was analyzed by the Tm value of the dissolution curve, which had good specificity and could detect five different virulence genes: aggR for EAEC, eae for EHEC and EPEC, LT for ETEC, ipaH for EIEC in the same reaction tube. The fecal simulated samples showed that the sensitivity reached 104~106 CFU/mL. The multiplex real-time PCR detection method established in this experiment is suitable for the preliminary screening of five kinds of Escherichia coli.
Diversity Of Antibiotic Resistant Bacteria Isolated From Cold Fresh Chicken Handlers’ Hands,
2019
Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Diversity Of Antibiotic Resistant Bacteria Isolated From Cold Fresh Chicken Handlers’ Hands, Huang Liujuan, Shao Yi, Feng Bo, Zhou Changyan
Food and Machinery
In order to investigate the potential migration of antibiotic resistant (ART) bacteria from cold fresh chicken to humans during handling in production and consumption, selective culture technology and IonS5TM XL sequencing platform were used for counting and diversity analysis of tetracycline-(TETr) and sulfamethoxazole-resistant bacteria (SULr) on the surface of six handlers’ hands contacting with cold fresh chicken products. The results showed that the two ART bacteria from the handlers’ hands significantly increased to 4.80×10～2.66×106 CFU/cm2 after contacting with cold fresh chicken. Furthermore, 21 and 19 genera of shared flora were identified in the …
Numerical Simulation Of Pulverizing Flow Field And Parameter Optimization Of The Mill,
2019
School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou, Guangdong 510641, China
Numerical Simulation Of Pulverizing Flow Field And Parameter Optimization Of The Mill, Mo Haijun, Ling Tao, Zhang Zejun
Food and Machinery
The Fluent software was used to simulate the pulverization flow field of the “pulverizaing-disc grinding” two-stage mill. The influence and law of the blade distribution scheme on the circulating flow field were compared and analyzed. The parameters of the pulverizing blade were optimized by orthogonal design method. The rules of the blade deflection angle, the blade length and the blade pitch on the pulverization effect were analyzed, and the influence of the blade rotation speed on the pulverization effect was further discussed. The material pulverization was experimented to obtain the best performance parameters of the Mill. Taking the pulverization rotating …
Construction Of Production Line Docking System Platform Based On Three-Dimensional Projection Matrix,
2019
Sichuan Finance and Economics Vocational College, Chengdu, Sichuan 610101, China
Construction Of Production Line Docking System Platform Based On Three-Dimensional Projection Matrix, Zhang Juanjuan, Shen Xiaoyu
Food and Machinery
Due to the problems of torsion and swing of worktable in food machinery processing enterprises, errors happen to the docking in the mechanical processing. Therefore, a platform for the production line docking system, based on three-dimensional projection matrix, was designed to proposed to reduce docking error. Firstly, the three-dimensional motion platform of the system adopted a stepping motor controlled by pulse signal to achieve the limit control of machine tool. Secondly, the laser control of the system was realized by controlling the laser switch and the output power of the laser. Thirdly, the displacement measurement technology of Doppler frequency difference …
Dynamics Analysis Of Carden Circular Panetary Mechanism,
2019
Institute of Strength and Vibration of Mechanical Structures, Yangtze University, Jingzhou, Hubei 434023, China
Dynamics Analysis Of Carden Circular Panetary Mechanism, Wang Bingbing, Li Meiqiu, Luo Jingbo, Fu Fangqin
Food and Machinery
According to the motion law of the carden planetary mechanism, the equation of motion of the mechanism is solved by geometric analysis, and the dynamic equation of the mechanism is established from the point of view of energy. The change rule of the input torque and output generalized force with time is obtained, and the load spectrum of carden planetary mechanism is qualitatively. The response law of the system is analyzed and the factors affecting the dynamic characteristics of the system are studied. By comparing the simulation with the theoretical calculation, the feasibility of the study is proved, providing design …
Evaluation Method Packaging Shapes Kansei Based On Computing With Words,
2019
College of Mechanical and Electrical Engineering, Zaozhuang University, Zaozhuang, Shandong 277160, China
Evaluation Method Packaging Shapes Kansei Based On Computing With Words, Ning Tingzhou, Zhang Jinzhi, Ge Meiqin
Food and Machinery
In order to accurately reflect the uncertainty of consumers' perceptual perception, this paper describes consumers' subjective judgment with hesitant fuzzy linguistic terminology set, analyses its operation rules, and constructs hesitant fuzzy CWW(computing with words) model. The case study shows that the aggregation of consumers' perceptual preferences in hesitant fuzzy terms and the realization of information aggregation through linguistic computation can help accurately reflect consumers' subjective perception, reduce consumers' uncertainty in perceptual judgment of packaging shape and support industrial designers to deal with consumers’ kansei opinions more accurately.
Study On The Change Of Texture Characteristics Of Water Buffalo Meat During Low Temperature Storage,
2019
Department of Food Science and Engineering, School of Biology and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, China; College of Food Science and Engineering, Huazhong Agricultural University, Wuhan, Hubei 4
Study On The Change Of Texture Characteristics Of Water Buffalo Meat During Low Temperature Storage, Li Chunyou, Fu Chunting, Yin Chaomin, Xu Jinrong, Huang Chengdu, Jin Guofeng
Food and Machinery
The changes of texture characteristics of Guangxi water buffalo meat buffalo meat was investigated during low temperature storage using texture profile analysis. Moreover, the correlation among various indexes of beef texture characteristics of buffalo meat was also analyzed. The results indicated that the hardness, elasticity, chewiness and adhesiveness of buffalo meat decreased continuously with the prolongation of storage time, but the cohesiveness of buffalo meat was increasing. During cryopreservation, there was a significantly positive correlation between the hardness of buffalo meat and characteristics of elasticity, adhesiveness, and chewiness (P＜0.05), but no significant correlation between the cohesiveness of buffalo meat and …
Optimization Of Flash Extraction Process And Structure Identification Ofpolysaccharide And Protein From Hericium Erinaceus,
2019
College of Food Science Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Optimization Of Flash Extraction Process And Structure Identification Ofpolysaccharide And Protein From Hericium Erinaceus, Zhang Xiong, Xiao Zhiyong, Huang Qun, Huang Xiang, Yang Ran, Chen Lei, Wang Yiwei
Food and Machinery
The polysaccharides and proteins from Hericium erinaceus were flashly extracted. On the basis of single factor experiments, the response surface methodology was applied to optimize the extraction conditions, and the structure of the polysaccharide and the subunit composition of the protein molecule were analyzed by infrared spectroscopy and SDS-PAGE respectively. The optimal condition for extraction of Hericium erinaceus polysaccharides (HEP) and proteins were: extracting time 80 s, liquid-solid ratio 17∶1 (mL/g), grinding granularity 80 meshes, extracting voltage 100 V, HEP yield rate (9.24±0.15)% and protein yield rate was 1.34%. The fourier transform infrared spectrum showed that HEP had absorption peaks …
Effect Of Different Pretreatments Methods On The Product Quality Of Explosion Puffing Drying For Purple Potato Crisps,
2019
Changsha University, Changsha, Hunan 410022, China
Effect Of Different Pretreatments Methods On The Product Quality Of Explosion Puffing Drying For Purple Potato Crisps, Wang Lan, Deng Bo, Deng Fangming
Food and Machinery
The objective was to investigate the effect of pretreatment methods on the characteristic of purple potato by explosion puffing drying. The effects were analyzed, from the slice thickness, concentration of citric acid solution, blanching time, freeze-thaw treatment and pre-drying time on moisture content, color difference, hardness, rehydration ratio and apparent density of explosion puffing drying purple potato. Results showed that the appropriate pretreatment conditions were as followed: slice thickness of 2 mm, color protection time by 0.2% citric acid solution for 20 min, water boiled at 100 ℃ for 1 min, freezing at -18 ℃ for 12 h. At room …
Effect Of Edible Mushroom Polysaccharide On Human Gut Microbiome,
2019
Central South University of Forestry and Technology, Changsha, Hunan 410004, China
Effect Of Edible Mushroom Polysaccharide On Human Gut Microbiome, Cheng Mengya, Yang Yalan, Yang Qiao, Feng Lulu, Huang Rengui, Zheng Lufei, Ren Jiali
Food and Machinery
Polysaccharide is one of the main bioactive compounds in these mushroom which had been generally studied to have the health-promoting properties of anti-oxidation, antitumor, anti-inflammatory, immunoregulation. With the heat wave of gut microbiome rising, how the edible mushroom polysaccharide affects human health through the gut microbiome has become the focus of attention. This review discussed the efficacy and structure of edible mushroom polysaccharides, the composition, distribution and function of human gut microbiome, and how the mushroom polysaccharides affect human health through regulating the gut microbiome. At the same time, preliminary suggestions for the follow-up study of edible mushroom polysaccharides were …
Progress On Main Virulence Factors And Pathogenic Mechanism Of Cronobacter Spp.,
2019
College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
Progress On Main Virulence Factors And Pathogenic Mechanism Of Cronobacter Spp., Fei Peng, Yang Tongxiang, Chen Xi, Xiang Jinle, Zhao Shengjuan, Xu Yunfeng, Zhou Lianxin, Guo Ling, Kang Huaibin
Food and Machinery
In this study, the role of major virulence factors in the pathogenic process of Cronobacter, including enterotoxin, outer membrane protein, gene Inv, and key enzyme RpfF were reviewed. Furthermore, the pathogenic mechanism was summarized from the following aspects, i. e. the adhesion of bacterial hair, the absorption ability of iron by Cronobacter, the utilization of sialic acid, the effects of biofilm on the protection, and the adhesion of Cronobacter, the effect of efflux system on the viability of Cronobacter in host gastrointestinal tract and desiccation resistance.
Progress In Research Of Flow Field Optimization In Quick Freezing Equipment,
2019
Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China; Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China
Progress In Research Of Flow Field Optimization In Quick Freezing Equipment, Zhu Yifan, Xie Jing
Food and Machinery
Quick freezing is an important technique for food preservation. The faster the freezing rate, the better the quality of frozen food after thawing. The greater the wind speed on the surface of the frozen product, the greater the heat exchange intensity and the faster the freezing rate. However, only increasing the fan speed will result in a decrease in fan efficiency and a decrease in flow field evenness, so the flow field needs to be optimized. The fan efficiency can be increased by setting the cone to the fan. The baffle can be arranged in the flow field to eliminate …
Opportunities And Challenges Of Pulses In The Sustainable Development Of Diets,
2019
Academy of National Food and Strategic Reserves Administration, Beijing 100037, China
Opportunities And Challenges Of Pulses In The Sustainable Development Of Diets, Tan Bin, Qiao Congcong
Food and Machinery
As one kind of sustainable diets, pulses have their own advantages and developmental opportunities, combined with some problems. In this review, the necessity and development direction of sustainable dietary development were discussed, and the advantages and problems of pulses as one kind of sustainable diets, and the development direction of pulses foods were summarized.
Effect Of Three Reducing Sugars On Emulsifying Property And Structure Of Maillard Products Of Kidney Bean Protein,
2019
College of Food and Bioengineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China; Key Laboratory of Processing Agricultural Products, Qiqihar, Heilongjiang 161006, China
Effect Of Three Reducing Sugars On Emulsifying Property And Structure Of Maillard Products Of Kidney Bean Protein, Lin Wei, Liu Xiaolan, Ren Jian, Gao Jian, Du Hong
Food and Machinery
The purpose of this study was to investigate the effect of sugars on the structural and emulsifying property of kidney bean protein modified by Maillard reaction. The results showed that the emulsifying activity and stability of kidney bean protein could be improved by Maillard reaction with glucose, fructose and lactose, and fructose was the best one. The emulsifying ability increased from 37.52 m2/g to 98.69 m2/g, and the emulsifying stability also increased from 16.88 min to 28.68 min; After Maillard reaction, the spatial structure of protein changed, which showed that the surface hydrophobicity increased, the content …
Optimization On Osborne Extracting Process For Mung Bean Albumin And Its Subunit Strip Determination,
2019
Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China;National Cereals Engineering Technology Research Center, Daqing, Heilongjiang 163319, China
Optimization On Osborne Extracting Process For Mung Bean Albumin And Its Subunit Strip Determination, Zhang Shu, Wang Changyuan, Tan Zhaoyin, Feng Yuchao
Food and Machinery
Taking the mung beans as raw materials, the Osborne classification method was used to extract albumin from mung bean. Based on the single factor test, using the L9 (34) orthogonal test, the effects were studied, from the extraction temperature, extraction time and ratio of material to liquid on the extraction rate of mung bean albumin. The subunit composition of mung bean albumin was analyzed by SDS-PAGE. The results showed that the optimal extraction process of mung bean albumin were: ratio of solid to liquid of 1∶10 (g/mL), extraction temperature of 45 ℃, extraction time of 150 min. …
Comparing Of Two Seperation Processes For Recovering Xylose Mother Liquor With Sequential Simulated Moving Bed Technology,
2019
National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Comparing Of Two Seperation Processes For Recovering Xylose Mother Liquor With Sequential Simulated Moving Bed Technology, Li Hongfei, Sun Daqing, Li Liangyu, Yu Wei, Zhang Liping
Food and Machinery
The two kinds of sepatation process for the xylose mother liquor of xylose, arabia sugar and glucose was studied which used the sequential simulated moving bed (SSMB) chromatographic separation equipment. In the optimal separation conditions of the separation process for two components, the purity of xylose and arabia sugar reached 86.30% and 88.41%, and recovery rate reached 85.20% and 89.26%. During the separation process of the three components, the purity of xylose and arabia sugar reached 77.73% and 79.94%, and the recovery rate reached 82.00% and 87.00%, respectively. Xylose mother liquor recovery processing was very difficult, the problem can be …
Optimization Of Xylanase Production By A Neurospora Sitophilathrough Solid State Fermentation,
2019
College of Food and Biotechnology, Qiqihar University, Qiqihar, Heilongjiang 161006, China
Optimization Of Xylanase Production By A Neurospora Sitophilathrough Solid State Fermentation, Duan Rui, Deng Yongping, Liu Xiaolan, Zheng Xiqun, Jiang Huanxiao, Ma Rui
Food and Machinery
Using vinegar as the main raw material to produce xylanase by solid-state fermentation of Neurospora sitophila. Using the activities of xylanase as indicators, the medium and culture conditions were optimized by single factor experiment and orthogonal experiment. Results: The medium consisted of 5 g of vinegar and 0.4 g of bean dregs. The initial pH of the medium was 5.5, 106 spores/Bottle of inoculum concentration and the solid-liquid ratio was 1∶3 (g/mL), and the culture medium was cultured at 28 ℃ for 3 d. The enzyme activity was 412.34 U/g under the optimized conditions, which was increased about 3.59 times …
Effects Of Glycosylation Modification On The Antioxidant Activity Of Casein-Enzymatic Hydrolysate,
2019
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Effects Of Glycosylation Modification On The Antioxidant Activity Of Casein-Enzymatic Hydrolysate, Zhao Yubin, Mu Qiuxia, Qu Liuqing, Cui Supin
Food and Machinery
In order to enhance the antioxidant activity of the casein hydrolysate, the enzymatic hydrolysate was modified by glycosylation with glucose, xylose and fructose, respectively. The effects of modification conditions on the antioxidant activity of the product were investigated under the mass ratio of enzymatic hydrolysate to sugar 1∶2. The results showed that the hydroxyl radical clearance rate reached the maximum at pH 7.0, which were 38.63% and 32.82%, respectively, and each was higher than the control. At pH 7.0, the reducing ability of the enzymatic hydrolysate was the strongest when modified by glycosylation with glucose or xylose. As the reaction …
Aerobic Acclimation Of Bifidobacterium Adolescentis And The Effect On Its Proliferation Of Different Oligosaccharides,
2019
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Aerobic Acclimation Of Bifidobacterium Adolescentis And The Effect On Its Proliferation Of Different Oligosaccharides, Li Yanze, Chen Xiajing, Zhang Shiang, Jin Xin, Xu Lei, Yao Di
Food and Machinery
In order to improve the situation on low number of living bacterium and the weak strain effect on Bifidobacterium adolescentis in fermented products, the anaerobic B. adolescentis was oxygen-resistant domesticated, by using anaerobic and aerobic alternating domesticated method, and the partial pressure of oxygen in the culture medium of B. adolescentis was gradually increased in the process of acclimation. The physiological characteristics before and after the domestication of B. adolescentis was determinated and the effects on its proliferation of the soybean oligosaccharides, xylooligosaccharide and oligomeric maltose were compared. The results showed that the sensitive degree to oxygen in B. adolescentis …
Genetic Diversity Analysis Of Common Bean Germplasms Based On Ssr Molecular Markers,
2019
College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
Genetic Diversity Analysis Of Common Bean Germplasms Based On Ssr Molecular Markers, Xia Chunyang, Du Jidao, Han Yiqiang, Sun Haoyue, Li Ming, Wu Hongbin, Zhang Qi, Yu Shenglong
Food and Machinery
The genetic diversity of 69 common bean germplasm resources was analyzed by SSR-PCR reaction system, and cluster analysis was carried out by UPGMA method. Seventeen pairs of SSR markers were detected and 55 allelic mutation loci were obtained, ranging from 2 to 5, with an average of 3.24 markers per pair. Seventeen pairs of primers had 17 polymorphic loci with 100% polymorphism. According to the cluster analysis results, the genetic similarity coefficient (GS) of the material ranged from 0.392 to 0.963, with an average of 0.659. 69 kinds of the beans seeds were divided into four groups at the genetic …
