Fgf-Signaling Is Compartmentalized Within The Mesenchyme And Controls Proliferation During Salamander Limb Development,
2019
University of Kentucky
Fgf-Signaling Is Compartmentalized Within The Mesenchyme And Controls Proliferation During Salamander Limb Development, Sruthi Purushothaman, Ahmed Elewa, Ashley W. Seifert
Biology Faculty Publications
Although decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how Sonic hedgehog (Shh) and Fibroblast growth factor (Fgf) signaling regulate limb development in the axolotl. We found that Shh-expressing cells contributed to the most posterior digit, and that inhibiting Shh-signaling inhibited Fgf8 expression, anteroposterior patterning, and distal cell proliferation. In addition to lack of a morphological AER, we found that salamander …
Towards A Mathematical Model Of Motility Using Dictyostelium Discoideum: Proteins And Geometric Features That Regulate Bleb-Based Motility,
2019
CUNY Graduate Center
Towards A Mathematical Model Of Motility Using Dictyostelium Discoideum: Proteins And Geometric Features That Regulate Bleb-Based Motility, Zully Santiago
Dissertations, Theses, and Capstone Projects
A variety of biological functions depend on actin organization. The organization of actin is tightly regulated by a plethora of extracellular and intracellular signaling, scaffolding, and actin-binding proteins. Dysfunctions in this regulation lead to immune diseases, increased susceptibility to pathogens, neurodegenerative diseases, developmental disorders, and cancer metastasis. A variety of actin-dependent processes, including cell motility, are regulated by several proteins of interest: Paxillin, a scaffolding protein; WASP, an actin nucleating protein; SCAR/WAVE, another WASP family actin nucleating protein; Talin, a cortex-to-membrane binding protein; Myosin II, an F-actin contracting motor protein; and Protein Kinase C, a protein kinase. D. discoideum cells …
The Influence Of Mir-322 On Skeletal Muscle Differentiation,
2019
University at Albany, State University of New York
The Influence Of Mir-322 On Skeletal Muscle Differentiation, Miles Alexander Soyer
Legacy Theses & Dissertations (2009 - 2024)
Skeletal muscle plays a crucial role in coordinating voluntary movement and accounts for nearly 50% of total body mass. Dysregulation in skeletal muscle development is known to cause muscle degenerative diseases including the devastating Duchenne Muscular Dystrophy (DMD). The majority of the biological studies investigating muscle development were based on myogenic transcription factors and signaling molecules including: Pax7, Myf5, MyoD, WNT, TGF-β and BMP. After the discovery of non-coding RNAs including microRNAs, it was postulated that these molecules could regulate gene expression and thus affect differentiation and development. MicroRNAs are small non-coding RNAs (~17-25 nucleotides) that regulate gene expression negatively …
Understanding The Molecular And Cellular Functions Of Odd-Skipped Related 1 In Outflow Tract Development,
2019
University of North Dakota
Understanding The Molecular And Cellular Functions Of Odd-Skipped Related 1 In Outflow Tract Development, Menglan Xiang
Theses and Dissertations
The cardiac outflow tract (OFT) is a transient conduit that connects the embryonic heart chambers to the vascular network. Transcription factor Osr1 promotes the proliferation and cell cycle progression of second heart field (SHF), an essential cell population that contribute to the developing OFT. In this study, we investigated the role of Osr1 in OFT development on cellular and molecular levels using a systems biology approach. We observed OFT rotation and elongation defects, as well as double-outlet right ventricle and overriding aorta as a result of SHF-specific deletion of Osr1. Using genetic inducible fate mapping, we showed that Osr1-expressing SHF …
The Functional Conservation Of Frazzled In Insects,
2019
University of Arkansas, Fayetteville
The Functional Conservation Of Frazzled In Insects, Benjamin Wadsworth
Graduate Theses and Dissertations
Axons in the developing embryo receive and react to signals that direct their growth to reach target tissues at specified locations. The signal pathways that direct midline crossing of axons during embryonic development have been comprehensively examined in the past years using the Drosophila ventral nerve cord or the spinal cord as a model system. A number of these signaling mechanisms are conserved, however disparities have been found between species in general strategy or the molecular signals controlling the response of axons to guidance cues.
The Netrin-Frazzled pathway has been shown to aid in midline crossing of axons in the …
Knocking Out A Negative Regulator Of Hedgehog Signaling Blocks Differentiation Of Cells Into Neurons,
2019
Western University
Knocking Out A Negative Regulator Of Hedgehog Signaling Blocks Differentiation Of Cells Into Neurons, Danielle Margaret Spice, Gregory M. Kelly Ph.D.
Western Research Forum
Hedgehog (Hh) signaling, one of many different protein signaling pathways found in mammals, is vital in many stage of neural development. A major negative regulator of Hh signaling is a protein known as Suppressor of Fused (SUFU), which acts to sequester the full length Gli transcription factors, proteins that can turn genes on and off, in the cytoplasm or facilitates its conversion to a repressive form. The P19 embryonal carcinoma cell line is a model of hind-brain neuronal differentiation and the involvement of Hh signaling, in particular the role of SUFU in this process has yet to be explored. We …
Glial Bridge Development: 26 Hpf,
2019
Smith College
Glial Bridge Development: 26 Hpf, Barresi Lab, Smith College
Glial Bridge
No abstract provided.
Glial Bridge Development: 24 Hpf,
2019
Smith College
Glial Bridge Development: 24 Hpf, Barresi Lab, Smith College
Glial Bridge
No abstract provided.
Glial Bridge Development: 30 Hpf,
2019
Smith College
Glial Bridge Development: 30 Hpf, Barresi Lab, Smith College
Glial Bridge
No abstract provided.
Glial Bridge Development: 28 Hpf,
2019
Smith College
Glial Bridge Development: 28 Hpf, Barresi Lab, Smith College
Glial Bridge
No abstract provided.
Slit1a Rescue: Role Of Slit1a, Hss1a,
2019
Smith College
Slit1a Rescue: Role Of Slit1a, Hss1a, Barresi Lab, Smith College
Role of slit1a
No abstract provided.
Slit1a Rescue: Role Of Slit1a, Hss1ayot,
2019
Smith College
Slit1a Rescue: Role Of Slit1a, Hss1ayot, Barresi Lab, Smith College
Role of slit1a
No abstract provided.
Slit1a Rescue: Role Of Slit1a, Non-Hss1ayot,
2019
Smith College
Slit1a Rescue: Role Of Slit1a, Non-Hss1ayot, Barresi Lab, Smith College
Role of slit1a
No abstract provided.
Slit1a Rescue: Role Of Slit1a, Wt,
2019
Smith College
Slit1a Rescue: Role Of Slit1a, Wt, Barresi Lab, Smith College
Role of slit1a
No abstract provided.
Validation Experiment: Wild Type, Raw, Gfap,
2019
Smith College
Validation Experiment: Wild Type, Raw, Gfap, Barresi Lab, Smith College
Validation Experiment
No abstract provided.
Validation Experiment: Wild Type, Psi,
2019
Smith College
Validation Experiment: Wild Type, Psi, Barresi Lab, Smith College
Validation Experiment
No abstract provided.
Glial Bridge Development: 22 Hpf,
2019
Smith College
Glial Bridge Development: 22 Hpf, Barresi Lab, Smith College
Glial Bridge
No abstract provided.
Validation Experiment: Wild Type, Probability,
2019
Smith College
Validation Experiment: Wild Type, Probability, Barresi Lab, Smith College
Validation Experiment
No abstract provided.
Validation Experiment: Wild Type, Raw, At,
2019
Smith College
Validation Experiment: Wild Type, Raw, At, Barresi Lab, Smith College
Validation Experiment
No abstract provided.
Validation Experiment: You-Too, Probability,
2019
Smith College
Validation Experiment: You-Too, Probability, Barresi Lab, Smith College
Validation Experiment
No abstract provided.
