University Of Arkansas, Chemistry And Biochemistry Department Graduate Student Publications, 2018- November 2023. 39p., 2023 University of Arkansas, Fayetteville
University Of Arkansas, Chemistry And Biochemistry Department Graduate Student Publications, 2018- November 2023. 39p., Jeremy Smith, Lutishoor Salisbury
University Libraries Faculty Publications and Presentations
This report provides a compilation of the research publications by the Chemistry and Biochemistry (CHBC) Graduate students for the period: 2018-November 2023. It includes publications by the CHBC graduates and those where a CHBC faculty was the main advisor. It includes a summary of the research. The listing is organized according to type of publications within specific years.
University Of Arkansas, Chemistry And Biochemistry Department Research Publications, 2014- November 2023. 107p., 2023 University of Arkansas, Fayetteville
University Of Arkansas, Chemistry And Biochemistry Department Research Publications, 2014- November 2023. 107p., Jeremy Smith, Lutishoor Salisbury
University Libraries Faculty Publications and Presentations
This report provides a compilation of the research publications by the Chemistry and Biochemistry faculty for the period: 2014 - November 2023. The information was gathered from major databases in science and technology including Web of Science, SciFinder, Reaxys, PubMed, IEEE Explore and Engineering Index. At least one author in each of the publications has the CHBC department as its affiliation. It includes a table summarizing the research. The listing is organized according to type of publications within specific years.
Microcystin Aids In Cold Temperature Acclimation: Differences Between A Toxic Microcystis Wildtype And Non-Toxic Mutant, 2023 The University of Tennessee, Knoxville
Microcystin Aids In Cold Temperature Acclimation: Differences Between A Toxic Microcystis Wildtype And Non-Toxic Mutant, Gwendolyn F. Stark, Robbie M. Martin, Laura E. Smith, Bofan Wei, Ferdi L. Hellweger, George S. Bullerjahn, R. Michael L. Mckay, Gregory L. Boyer, Steven W. Wilhelm
Great Lakes Institute for Environmental Research Publications
For Microcystis aeruginosa PCC 7806, temperature decreases from 26 °C to 19 °C double the microcystin quota per cell during growth in continuous culture. Here we tested whether this increase in microcystin provided M. aeruginosa PCC 7806 with a fitness advantage during colder-temperature growth by comparing cell concentration, cellular physiology, reactive oxygen species damage, and the transcriptomics-inferred metabolism to a non-toxigenic mutant strain M. aeruginosa PCC 7806 ΔmcyB. Photo-physiological data combined with transcriptomic data revealed metabolic changes in the mutant strain during growth at 19 °C, which included increased electron sinks and non-photochemical quenching. Increased gene expression was observed for …
Canagliflozin Ameliorates Autistic-Like Features And Mitigates Brain Oxidative Stress Levels In Valproic Acid-Induced Autism In Rats, 2023 United Arab Emirates University
Canagliflozin Ameliorates Autistic-Like Features And Mitigates Brain Oxidative Stress Levels In Valproic Acid-Induced Autism In Rats, Mohammad Moutaz Nakhal
Theses
Autism spectrum disorder (ASD) is a neurodevelopmental disease with a substantially increasing incidence rate. It is mainly characterized by repetitive behavior, intellectual difficulties, social communication and interactions deficits. Many medications, dietary supplements, and behavioral treatments have been recommended for ASD management, however, there is no cure yet. Recent studies have examined the therapeutic potential of the sodium-glucose cotransporter 2 (SGLT2) inhibitors in neurodevelopmental diseases, based on their proved anti-inflammatory and antioxidant effects. The main objective of this study is to assess the ability of canagliflozin in improving the behavioural characteristics of autistic rats and investigate the efficacy of canagliflozin in …
Changes In Lipid Profiles Of Epileptic Mouse Model, 2023 University of Nebraska-Lincoln
Changes In Lipid Profiles Of Epileptic Mouse Model, Alicia Johnson, Ryan A. Grove, Deepak Madhavan, Cory Honsinger Thomas Boone, Camila Pereira Braga, Hannah Kyllo, Kaeli Samson, Timothy Simeone, Tomáš Helikar, Corrine K. Hanson, Jiri Adamec
Department of Biochemistry: Faculty Publications
Introduction—Approximately 1% of the world’s population is impacted by epilepsy, a chronic neurological disorder characterized by seizures. One-third of epileptic patients are resistant to AEDs, or have medically refractory epilepsy (MRE). One non-invasive treatment that exists for MRE includes the ketogenic diet, a high-fat, low-carbohydrate diet. Despite the KD’s success in seizure attenuation, it has a few risks and its mechanisms remain poorly understood. The KD has been shown to improve metabolism and mitochondrial function in epileptic phenotypes. Potassium channels have implications in epileptic conditions as they have dual roles as metabolic sensors and control neuronal excitation.
Objectives—The …
Substrate Specificity In Abc Transporters Using The E. Coli Methionine Import System, 2023 The University of San Francisco
Substrate Specificity In Abc Transporters Using The E. Coli Methionine Import System, John H. Guardado
Featured Student Work
ATP-binding cassette (ABC) transporters use the energy of ATP to move substrates across membranes against a concentration gradient. The role of ABC transporters is crucial in several essential cellular functions and mutations in ABC transporters in humans have been linked to several conditions, including cystic fibrosis, liver disease, and diabetes. Despite their central roles in homeostasis, the mechanism of ABC transporters remains poorly understood. Our research is focused on studying an ABC importer in E. coli, as a model system, to examine the mechanism of substrate specificity and transport. The bacterial methionine import system consists of a membrane-embedded transporter, MetNI, …
Acetylation Regulates Thioredoxin Reductase Activity And Oligomerization, 2023 Western University
Acetylation Regulates Thioredoxin Reductase Activity And Oligomerization, David E. Wright
Electronic Thesis and Dissertation Repository
The Thioredoxin (Trx) system provides the cell with robust defense against oxidative stress and regulates the function of nearly every cellular process through the reduction-oxidation (redox) regulation of proteins. The Trx system is involved in the development of many diseases ranging from cancer to cardiovascular disorders. Thioredoxin reductase (TrxR) is the key enzyme in the Trx system and contains the 21st genetically encoded amino acid, selenocysteine (Sec). There were multiple experimentally identified TrxR acetylation sites with an unknown effect on TrxR activity. My thesis tested the hypothesis that programmed protein acetylation will enhance the activity of TrxR1. I used …
The Effects Of Vaping Coil Temperature On The Viability Of Veillonella Parvula And Streptococcus Mutans, 2023 Harrisburg University of Science and Technology
The Effects Of Vaping Coil Temperature On The Viability Of Veillonella Parvula And Streptococcus Mutans, Marvelous O. Aluko
Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity
No abstract provided.
Using Hyperosmotic Shock To Study The Cell Wall Of Myxococcus Xanthus, 2023 Harrisburg University of Science and Technology
Using Hyperosmotic Shock To Study The Cell Wall Of Myxococcus Xanthus, Ian Sabol
Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity
No abstract provided.
Effects Of Growth Rate On Peptidoglycan Crosslink Density Of E. Coli Using Fluorescent Labeling, 2023 Harrisburg University of Science and Technology
Effects Of Growth Rate On Peptidoglycan Crosslink Density Of E. Coli Using Fluorescent Labeling, Morgan Olszewski
Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity
Peptidoglycan (PG) is a polymer composed of polysaccharides and crosslinked peptide chains found in bacterial cell walls. It helps to protect the cell from environmental stress and maintain cell morphology throughout its life cycle and further generations. The PG is made up of two sugars, N-acetyl muramic acid (NAM) and N-acetyl glucosamine (NAG). NAM and NAG are connected by glycosidic linkages to form repeated chains. The chains are formed in layers, which are interconnected via a polypeptide stem linked by a peptide bridge. During cell growth, continuous turnover of existing PG occurs by severing existing crosslinks, inserting new PG into …
Thermodynamic Frustration Of Tad2 And Prr Contribute To Autoinhibition Of P53, 2023 University of South Florida
Thermodynamic Frustration Of Tad2 And Prr Contribute To Autoinhibition Of P53, Emily Gregory
USF Tampa Graduate Theses and Dissertations
The intrinsically disordered transcription factor and tumor suppressor p53 binds to promoter response element DNA upon cellular stress and activates genes associated with cell cycle arrest, senescence, and apoptosis. Disruption of sequence specific binding to target gene promoters is heavily implicated in human health, where a majority of cancers contain mutations localized to the DNA binding domain (DBD) of p53. p53 DNA binding is regulated by posttranslational modifications, associations with cellular factors, and by an autoinhibitory intramolecular interaction. The autoinhibitory intramolecular interaction occurs when the disordered N-terminal transactivation domain (TAD) interacts with the ordered DBD. Previous work in the Daughdrill …
Bola3 And Nfu1 Link Mitoribosome Iron–Sulfur Cluster Assembly To Multiple Mitochondrial Dysfunctions Syndrome, 2023 University of Miami Miller School of Medicine
Bola3 And Nfu1 Link Mitoribosome Iron–Sulfur Cluster Assembly To Multiple Mitochondrial Dysfunctions Syndrome, Hui Zhong, Alexandre Janer, Oleh Khalimonchuk, Hana Antonicka, Eric A. Shoubridge, Antoni Barrientos
Department of Biochemistry: Faculty Publications
The human mitochondrial ribosome contains three [2Fe–2S] clusters whose assembly pathway, role, and implications for mitochondrial and metabolic diseases are unknown. Here, structure-function correlation studies show that the clusters play a structural role during mitoribosome assembly. To uncover the assembly pathway, we have examined the effect of silencing the e xpression of Fe–S cluster biosynthetic and delivery factors on mitoribosome stability. We find that the mitoribosome receives its [2Fe–2S] clusters from the GLRX5-BOLA3 node. Additionally, the assembly of the small subunit depends on the mitoribosome biogenesis factor METTL17, recently reported containing a [4Fe–4S] cluster, which we …
On The Origins Of Life — Modelling The Initial Stages Of Complex Coacervate Droplet Formation, 2023 Western University, London, ON
On The Origins Of Life — Modelling The Initial Stages Of Complex Coacervate Droplet Formation, Yixuan Wu
Western Libraries Undergraduate Research Awards (WLURAs)
Coacervate droplets are considered a plausible model for protocells due to their spontaneous formation and ability to compartmentalize macromolecules such as nucleic acid and peptides. Although experimental studies have observed and synthesized coacervates under different laboratory conditions, little is known about their structure. Here we present atomistic molecular dynamic simulations of the interactions between water and oppositely charged proteins that cluster together in a salt-dependent process. Observing such liquid-liquid phase separation on an atomic level would serve as a model for the initial stages of complex coacervate formation. Molecular Dynamics was used to compute diagnostics of the structure at different …
Short-Term Versus Long-Term Effects Of Nitrogen Addition And Warming On Ecosystem N Dynamics In A Grass-Dominated Temperate Old Field, 2023 Western University
Short-Term Versus Long-Term Effects Of Nitrogen Addition And Warming On Ecosystem N Dynamics In A Grass-Dominated Temperate Old Field, Benjamin F. A. Souriol
Electronic Thesis and Dissertation Repository
Increased atmospheric nitrogen (N) deposition and climate warming are both anticipated to influence the ecosystem N dynamics of northern temperate ecosystems substantially over the next century. Nevertheless, in field experiments with N addition and warming treatments, temporal scale can play an important role in determining the extent of treatment effects on N dynamics, and it is unclear to what extent the results of short-term studies can be extrapolated to responses over longer time scales. I compared the short-term versus long-term effects of N addition and warming on net N mineralization, N leaching, and N retention in a grass-dominated old field. …
Novel Treatments For Pxe: Targeting The Systemic And Local Drivers Of Ectopic Calcification, 2023 Thomas Jefferson University
Novel Treatments For Pxe: Targeting The Systemic And Local Drivers Of Ectopic Calcification, Ida Joely Jacobs, Qiaoli Li
Department of Biochemistry and Molecular Biology Faculty Papers
Pseudoxanthoma elasticum (PXE) is a heritable multisystem ectopic calcification disorder. The gene responsible for PXE, ABCC6, encodes ABCC6, a hepatic efflux transporter regulating extracellular inorganic pyrophosphate (PPi), a potent endogenous calcification inhibitor. Recent studies demonstrated that in addition to the deficiency of plasma PPi, the activated DDR/PARP signaling in calcified tissues provides an additional possible mechanism of ectopic calcification in PXE. This study examined the effects of etidronate (ETD), a stable PPi analog, and its combination with minocycline (Mino), a potent inhibitor of DDR/PARP, on ectopic calcification in an Abcc6-/- mouse model of PXE. Abcc6-/- mice, at 4 weeks of …
Molecular Event In Hrp Apoptosis, 2023 The University of Texas Rio Grande Valley
Molecular Event In Hrp Apoptosis, Laura Valdez
Research Colloquium
Background: Human retinal pericytes (HRP) are contractile cells providing support for endothelial cells of capillaries, essential in the regulation of retinal vasculature. Early stages of DR are characterized by the loss of HRP, leading to angiogenesis. Our preliminary studies identified monocyte-derived macrophages secrete TGF-β1, inducing the expression and secretion of a TGFβ1-Induced, pro-apoptotic BIGH3 protein leading to apoptosis of HRP. Based on a preliminary study in renal cells (unpublished data), CTP with an RGD domain is released from BIGH3 by proteolysis leading to renal cell apoptosis. In the present study, we employed Western Blots to determine if a similar molecular …
Irbp In Diabetic Retinopathy – Cell Culture & Ophthalmic Pathology Studies, 2023 The University of Texas Rio Grande Valley
Irbp In Diabetic Retinopathy – Cell Culture & Ophthalmic Pathology Studies, Laura Valdez
Research Colloquium
Background: Interphotoreceptor-Retinoid-Binding-Protein (IRBP) is restricted to the subretinal space by the external limiting membrane and RPE zonulae occludens. Its expression is reduced in diabetic retinopathy (DR) and increased expression is protective. Hypoxia decreases viable conelike photoreceptors (661W) but increased VEGF, suggesting a role of photoreceptors in proliferative DR (PDR) (Rodriguez et al, Arch Clin Exp. Ophthal 3:23,2021). Here, we investigated the effects of high glucose and VEGF on IRBP expression by 661W and rod-like Y79 cells and distribution of IRBP and albumin in human globes with PDR and NPDR.
Methods: 661W and Y79 cells were cultured in DMEM or RPMI …
Xrk3f2 Inhibits P62 Signaling And Augments Myeloma Killing By Proteasome Inhibitors, 2023 DePauw University
Xrk3f2 Inhibits P62 Signaling And Augments Myeloma Killing By Proteasome Inhibitors, Ellen Trautman, Colin Crean, David Halladay, Attaya Suvannasankha
Annual Student Research Poster Session
Background:
Despite advancements in therapy, multiple myeloma (MM) remains an incurable blood cancer. Our mission is to maximize the efficacy of a primary treatment for myeloma, proteasome inhibitors (PIs) which cause intracellular waste buildup, leading to ER stress and cell death. p62(sequestosome-1) provides an alternate pathway when the proteasome is inhibited, by breaking down cytotoxic material via autophagy. Upregulation of p62 is associated with PI resistance. We identified a small molecule, XRK3F2, that binds to the ZZ domain of p62 and inhibits its autophagic function. We hypothesized that XRK3F2 would improve MM killing when combined with PIs.
Methods Used:
We …
Examining The Ph Switch Of Copper-Zinc Superoxide Dismutase Mutant A4v, 2023 St. Mary's University
Examining The Ph Switch Of Copper-Zinc Superoxide Dismutase Mutant A4v, Ty Jungman
Honors Program Theses and Research Projects
Amyotrophic lateral sclerosis(ALS) is a neurodegenerative disease that causes progressive loss to motor neurons. Buildup of superoxide anions (O2-) and aggregation of superoxide dismutase (SOD) is thought to be probable causes of damage to the neurons. This damage results in the progressive loss of function in the neurons resulting in flaccid paralysis. Superoxide anions were originally thought to cause irreparable damage to the motor neurons; however, recent studies have shown that the aggregation of SOD causes damage to the cell. Furthermore, the ability for the SOD1 to get metalated to dissuade aggregation has become an important topic of study in …
Sars-Cov-2: Tale Of A Microscopic Murderer, 2023 Collin College
Sars-Cov-2: Tale Of A Microscopic Murderer, Josiah P. Garner
Quest
Independent Study
Research in progress for BIOL1406: Biology for Science Majors I
Faculty Mentor: Amina Tassa, Ph.D.
I am delighted to introduce Josiah Garner’s “SARS-CoV-2: Tale of a Microscopic Murderer.” This independent study assignment explores the impact of a novel, deadly, and worldwide virus. The assignment also examines the fast development of vaccines to control the spread and reduce the symptoms of the virus.
Josiah’s paper focuses on the early history of the emergence of COVID-19, the world response, and vaccine development. He demonstrates critical thinking skills and effectively utilizes various research methods to obtain and communicate his information. Josiah …