Derivation Of Hill's Equation From Scale Invariance,
2012
Department of Mathematical Sciences, University of Texas at El Paso
Derivation Of Hill's Equation From Scale Invariance, Andres Ortiz^, Vladik Kreinovich*
COURI Symposium Abstracts, Spring 2012
No abstract provided.
Interaction Of Trypanosoma Cruzi Sterol 14-Alpha-Demethylase Cyp51 With Ru-Azole Derivatives,
2012
Department of Biological Sciences, The University of Texas at El Paso
Interaction Of Trypanosoma Cruzi Sterol 14-Alpha-Demethylase Cyp51 With Ru-Azole Derivatives, Arturo Ortega^, Rosa A. Maldonado*, Roberto A. Sanchez-Delgado, Hugues Ouellet*
COURI Symposium Abstracts, Spring 2012
No abstract provided.
Cholesterol Side-Chain Degradation Enzymes Of Mycobacterium Tuberculosis,
2012
Department of Biological Sciences, University of Texas at El Paso
Cholesterol Side-Chain Degradation Enzymes Of Mycobacterium Tuberculosis, Brian Villalba^, Itzel Chavarria, Bernardo Terres, Johnathan Abou-Fadel, Rhone A. Mendoza, Hugues Ouellet*
COURI Symposium Abstracts, Spring 2012
No abstract provided.
Engineering The Human Immunodeficiency Virus To Infect Drosophila Melanogaster Cells,
2012
Department of Biological Sciences, University of Texas at El Paso
Engineering The Human Immunodeficiency Virus To Infect Drosophila Melanogaster Cells, Laura F. Porras ^, Elizabeth R. Aguilera, Eduardo Urias, Daniel Reyes, Adarsh Saheba, Manuel Llano *
COURI Symposium Abstracts, Spring 2012
HIV continues to infect at pandemic levels despite the significant progress in research, treatment and prevention. Advances in anti-HIV retroviral drugs have led to significant reduction in AIDS-related deaths, delayed the progression of the HIV infection to AIDS and diminished the rates of HIV transmission. However, the current antiretroviral treatment frequently encounters drug-resistant viruses due to the high mutation rate of HIV proteins. Therefore, identification of cellular cofactors of HIV infection is critical to identify new targets for anti-HIV drug development. However, the genetic complexity of mammalian cells imposes additional obstacles to the identification of HIV-1 cellular cofactors. To mitigate …
1-Deoxy-D-Xylulose-5-Phosphate Synthase (Dxs) Mechanistic Study And Its Implication In The Development Of Novel Antibiotics And Antimalarials,
2012
University of South Florida
1-Deoxy-D-Xylulose-5-Phosphate Synthase (Dxs) Mechanistic Study And Its Implication In The Development Of Novel Antibiotics And Antimalarials, Sumit Handa
USF Tampa Graduate Theses and Dissertations
Isoprenoids are the largest family of biologically active compounds, synthesized by five carbon subunits namely isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). For long time the mevalonate-dependent (MVA) pathway has been considered as the sole source of IPP and DMAPP, until recently a new non-mevalonte dependent (NMVA) pathway was discovered. This new pathway utilizes entirely different set of enzymes for isoprenoids synthesis and don't have any homologues in humans. NMVA pathway is the only source of isoprenoids for certain eubacteria, parasite and plants. Absence of the NMVA pathway in higher organisms has opened a new platform for the development of …
Glycosylation Of Human Cyclooxygenase-2 (Cox-2) Decreases The Efficacy Of Certain Cox-2 Inhibitors.,
2012
Department of Natural Sciences and Mathematics, Dominican University of California
Glycosylation Of Human Cyclooxygenase-2 (Cox-2) Decreases The Efficacy Of Certain Cox-2 Inhibitors., Mary B. Sevigny, Kamara Graham, Esmeralda Ponce, Maggie Louie, Kylie Mitchell
Natural Sciences and Mathematics | Faculty Scholarship
Prostanoids play an important role in a variety of physiological and pathophysiological processes including inflammation and cancer. The rate-limiting step in the prostanoid biosynthesis pathway is catalyzed by cyclooxygenase-2 (COX-2). COX-2 exists as two glycoforms, 72 and 74 kDa, the latter resulting from an additional glycosylation at Asn(580). In this study, Asn(580) was mutated, and the mutant and wild-type COX-2 genes were expressed in COS-1 cells to determine how glycosylation affects the inhibition of COX-2 activity by aspirin, flurbiprofen, ibuprofen, celecoxib, and etoricoxib. Results indicate that certain inhibitors were 2-5 times more effective at inhibiting COX-2 activity when the glycosylation …
The Relationship Between Live Coral And Macroalgae In South Caicos As Influenced By Herbivorous Fishes,
2012
University of Richmond
The Relationship Between Live Coral And Macroalgae In South Caicos As Influenced By Herbivorous Fishes, Sarah Byce
Honors Theses
Marine life is the basis for most industries in the Turks and Caicos Islands, located on the outskirts of the Caribbean Sea. Few studies have been carried out to assess the current status of reefs in this region, especially those off the shore of South Caicos Island. The AGRRA v.4.0 methodologies were employed create baseline assessments of stony corals, macroalgae and associated fishes and to explore the relationships between these key reef organisms. This study was based on 10 different locations both inside and outside of Marine Protected Areas (MPAs) and including exposed and sheltered reefs in proximity to South …
Understanding The Auto-Regulation Of Leukotriene Biosynthesis A Model Of Inactivation For 5-Lipoxgenase,
2012
Louisiana State University
Understanding The Auto-Regulation Of Leukotriene Biosynthesis A Model Of Inactivation For 5-Lipoxgenase, Erin E. Schexnaydre
Honors Capstones
No abstract provided.
Validation On Sodium Channel Dependence By Electrical Stimulation-Induced Dopamine Release,
2012
Department of Psychology, University of Texas at El Paso
Validation On Sodium Channel Dependence By Electrical Stimulation-Induced Dopamine Release, Alice E. Hernandez ^, Mabel N. Terminel, Edward Castaneda *
COURI Symposium Abstracts, Spring 2012
No abstract provided.
Elucidation Of The Pax/Six Gene Regulatory Network In Ephydatia Muelleri,
2012
University of Richmond
Elucidation Of The Pax/Six Gene Regulatory Network In Ephydatia Muelleri, Anna Rued
Honors Theses
The evolution of gene regulatory networks accounts for much of the diversity we observe in the animal kingdom. In particular, the Pax/Six/Eya/Dac gene network has been known to play critical roles in development of all bilaterians and cnidarians. However, this network has not been characterized in Porifera. These studies focus on tracing the evolutionary history of the PSED network back to the most basal organism in the metazoan phylogeny through demonstrating the presence of a direct gene regulatory network between PaxB and Six1/2 orthologs in sponges. To this end, putatitve Six1/2 cis- regulatory elements were identified while the PaxBprd transcription …
Design Of In Vivo Assays For Study Of Transport, Biocompatibility And Toxicity Of Nanoparticles,
2012
Old Dominion University
Design Of In Vivo Assays For Study Of Transport, Biocompatibility And Toxicity Of Nanoparticles, Kerry Jean Lee
Theses and Dissertations in Biomedical Sciences
This dissertation focuses on the design of new in vivo assays for study of transport, biocompatibility and toxicity of nanoparticles (NPs) in zebrafish embryos. We synthesized and purified spherical silver (Ag) NPs with diameters, ranging from 12 to 95 nm, that are stable (non-aggregated) in egg-water media. We developed new imaging approaches to characterize the sizes of single Ag NPs in zebrafish embryos at nanometer resolution by measuring their size-dependent plasmonic spectra and scattering intensity using dark-field optical microscopy and spectroscopy (DFOMS). We used single Ag NPs because they exhibit the high quantum yield (QY) of Rayleigh scattering and resist …
Alteration In 5ht1a Receptor Activity From A Prenatal Exposure To Dexamethasone In A Stressed And Non-Stressed Adult Male Rat,
2012
Bucknell University
Alteration In 5ht1a Receptor Activity From A Prenatal Exposure To Dexamethasone In A Stressed And Non-Stressed Adult Male Rat, Darshan S. Shah
Honors Theses
Synthetic glucocorticoids (GC) are used as a clinical therapeutic to stimulate lung development in fetuses that present the risk of preterm delivery. Previous studies have shown that a prenatal exposure to Dexamethasone (DEX) causes a disturbance in normal GC mediation of neuritic outgrowth, cell signaling, and serotonergic systems. Our hypothesis is that a prenatal exposure to DEX during the third trimester of pregnancy alters 5HT1A receptor function. Pregnant dams were injected daily with 150μg/ml/kg of DEX from gestation day 14 through 19. Control dams were treated with and equal volume of saline. Swim stress followed by elevated plus maze testing …
The Optimization Of A One-Pot Heteroconjugate Addition-Oxidation-Diels-Alder Reaction,
2012
University of Richmond
The Optimization Of A One-Pot Heteroconjugate Addition-Oxidation-Diels-Alder Reaction, Christina Vivelo
Honors Theses
Ynoate esters are ideal reagents for one-pot reactions due to their ability to undergo multiple addition reactions in one flask. Ethyl propiolate undergoes a heteroconjugate addition reaction with aromatic thiol nucleophiles, producing an enoate which is then oxidized by m-CPBA and is able to undergo a Diels–Alder reaction with cyclopentadiene. The work presents the optimization of a three-step heteroconjugate addition-oxidation-Diels–Alder reaction to yield cyclic compounds favoring endo stereochemistry, which may be used in further synthesis of biologically active compounds such as (+)-Methyl-5-epi-Shikimate or Deipeptyl-IV-Peptidase inhibitor.
The Role Of Histidines In Neurosteroid Binding Of Nmda Glun2b And D Subunits,
2012
University of Richmond
The Role Of Histidines In Neurosteroid Binding Of Nmda Glun2b And D Subunits, Sarah Rhoads
Honors Theses
Implicit in cellular mechanisms essential for learning and memory, NMDA receptors are heterotetrameric members of the family of ionotropic glutamate receptors. Activation of the glutamate-specific receptor subunits results in the opening of ion channels, triggering an excitatory pathway, which can be moderated by certain endogenous and exogenous compounds. Previous research with the endogenous neurosteroids pregnenolone sulfate (PS) and 3α-hydroxy-5-β-20-one sulfate (PregS) have indicated that the binding of these neurosteroids to NMDA receptors containing the four A-D isoforms of the GluN2 subunit affects current flow through them1. Binding of PS to NMDA receptors containing GluN2A or GluN2B subunits causes an increased …
Determination Of The Atomic Resolution Structure Of A Dna Polymerase I Isolated From Rhodothermus Marinus,
2012
University of Richmond
Determination Of The Atomic Resolution Structure Of A Dna Polymerase I Isolated From Rhodothermus Marinus, Natalie S. Omattage
Honors Theses
DNA polymerase I employs a multistep mechanism for sorting correctly paired nucleotides from mismatches. We aim to characterize reaction intermediates during nucleotide selection to better understand how this class of enzymes achieves high DNA replication fidelity. DNA polymerase I from R. marinus contains an unusual and disruptive proline in the mobile O helix near the active site. To characterize this enzyme, the structure of the large (5´-to-3´ exo-deficient) fragment of the R. marinus DNA polymerase I (RF) was solved to 2.95 Å (R = 0.234) using multi-wavelength anomalous dispersion. Alignment …
Composition And Evolution Of The Vertebrate And
Mammalian Selenoproteomes,
2012
Brigham and Women’s Hospital and Harvard Medical School
Composition And Evolution Of The Vertebrate And Mammalian Selenoproteomes, Marco Mariotti, Perry G. Ridge, Yan Zhang, Alexei V. Lobanov, Thomas H. Pringle, Roderic Guigo, Dolph L. Hatfield, Vadim N. Gladyshev
Department of Biochemistry: Faculty Publications
Background: Selenium is an essential trace element in mammals due to its presence in proteins in the form of selenocysteine (Sec). Human genome codes for 25 Sec-containing protein genes, and mouse and rat genomes for 24.
Methodology/Principal Findings: We characterized the selenoproteomes of 44 sequenced vertebrates by applying gene prediction and phylogenetic reconstruction methods, supplemented with the analyses of gene structures, alternative splicing isoforms, untranslated regions, SECIS elements, and pseudogenes. In total, we detected 45 selenoprotein subfamilies. 28 of them were found in mammals, and 41 in bony fishes. We define the ancestral vertebrate (28 proteins) and mammalian …
Correction To Fully Enclosed Microfluidic Paper-Based Analytical Devices,
2012
California Polytechnic State University, San Luis Obispo
Correction To Fully Enclosed Microfluidic Paper-Based Analytical Devices, Kevin M. Schilling, Anna L. Lepore, Jason A. Kurian, Andres W. Martinez
Chemistry and Biochemistry
There is an error in the units of the concentrations of potassium iodide and trehalose described in the experimental details on page 1581. The correct concentrations are 0.6 M potassium iodide and 0.3 M trehalose.
Connections Between Sphingosine Kinase And Phospholipase D In The Abscisic Acid Signaling Pathway In Arabidopsis,
2012
University of Missouri-St. Louis
Connections Between Sphingosine Kinase And Phospholipase D In The Abscisic Acid Signaling Pathway In Arabidopsis, Liang Guo, Girish Mishra, Jennifer E. Markham, Maoyin Li, Amanda Tawfall, Ruth Welti, Xuemin Wang
Department of Biochemistry: Faculty Publications
Background: Sphingosine kinase (SPHK) and phospholipaseD(PLD) produce different lipid mediators involved in abscisic acid (ABA) response.
Results: Ablation of SPHKs and PLDα1 attenuates ABA-induced production of LCBPs and PA. Phyto-S1P closes stomata in sphk1, sphk2, but not in pldα1, whereas PA closes stomata in all mutants.
Conclusion: SPHK acts upstream of PLDα1, whereas PLDα1 promotes SPHK.
Significance: The roles of lipid messengers in the ABA signaling pathway are clarified.
Follicular Dendritic Cells, Human Immunodeficency Virus Type 1, And Alpha 1 Antitrypsin,
2012
Brigham Young University - Provo
Follicular Dendritic Cells, Human Immunodeficency Virus Type 1, And Alpha 1 Antitrypsin, Xueyuan Zhou
Theses and Dissertations
HIV/AIDS is raging and causing millions of deaths around the world. The major challenge in treating HIV/AIDS is the establishment of HIV reservoirs where the viruse escapes both drug and immune system attempts at eradication. Throughout the course of HIV/AIDS, productive HIV infection occurs primarily in the lymphoid follicles or germinal centers (GC) surrounding follicular dendritic cells (FDC). In the GCs, FDCs trap and maintain infectious HIV for years and provide these infectious viruses to the host cells. FDCs also attract B and T cells into the GCs and increase the ability of CD4+ T cells to be infected. Additionally, …
Novel Phosducin-Like Protein Binding Partners: Exploring Chaperone And Tumor Suppressor Protein Interactions,
2012
Brigham Young University - Provo
Novel Phosducin-Like Protein Binding Partners: Exploring Chaperone And Tumor Suppressor Protein Interactions, Amy Jetaun Gray
Theses and Dissertations
Many proteins cannot fold into their native state without the assistance of one or more molecular chaperones. Chaperonins are an essential class of chaperones that provide an isolated chamber for proteins to fold. CCT, a group II chaperonin found in eukaryotes assists in the folding of actins, tubulins, and many other cellular proteins. PhLP1 is a member of the phosducin protein family that assists CCT in the folding of Gβ and its subsequent assembly with Gγ. However, previous studies have not addressed the scope of PhLP1 and CCT-mediated Gβγ; assembly. The data presented in Chapter 2 shows that PhLP1 plays …
