Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay,
2010
Old Dominion University
Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay, D. T. Gauthier, K. S. Reece, J. Xiao, M. W. Rhodes, H. I. Kator, R. J. Latour, C. F. Bonzek, J. M. Hoenig, W. K. Vogelbein
Biological Sciences Faculty Publications
Striped bass (Morone saxatilis) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli). Mycobacterium …
Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata).,
2010
Universiti Malaya
Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah
Student Works (2010-2019)
Channa striata (better known as Haruan in Malaysia), is a freshwater species that is well known as a protein source and also for its pharmaceutical value among wounded patients. Unfortunately stocks have declined over the past few years, thus an in-depth study is needed to rule out genetic irregularities that might be causing the slow extinction of the species. This study was conducted for the purpose of studying the population diversity and genetic structure of Channa striata (striped snakehead) in seven different locations around the peninsular of Malaysia. Microsatellite markers were isolated and identified using Random Amplified Microsatellite (RAM) primers. …
Isolation And Development Of Microsatellite Markers Of Channa Striata.,
2010
Universiti Malaya
Isolation And Development Of Microsatellite Markers Of Channa Striata., Faiza Omar
Student Works (2010-2019)
Channa striata, locally known as haruan has long been regarded as a valuable edible fish and it is also consumed for its putative effects on wound healing. The main objective of the project was to develop microsatellite markers for use in the study of genetic diversity of the wild Channa striata populations in Malaysia. In this study, seven new microsatellite loci were isolated from Channa striata using a Random Amplified Microsatellite (RAMs) based technique which was an efficient and reliable method. Ten primers were used in population genetic studies of Channa striata.Sixty sets of microsatellite primer pairs were used in …
Biofouling And Short-Term Dynamics On Fish Cage Netting In Relation To Fish Rearing And Environmental Factors.,
2010
Universiti Malaya
Biofouling And Short-Term Dynamics On Fish Cage Netting In Relation To Fish Rearing And Environmental Factors., John Madin
Student Works (2010-2019)
Four studies were conducted in a floating net-cages farm at Matang Mangrove Forest Reserve (MMFR), Perak, Malaysia, to elucidate the biofouling assemblages on cage nettings and factors that influence their development. In the first study (Chapter 3),biofouling on 1.6 cm mesh net panels (size 0.2 m x 2 m) suspended inside (homemade pellet ‘P’, trash fish ‘T’) and outside (O) experimental net-cages in a fish farm were monitored every week until net openings were completely occluded by macrofouling organisms. Eight species (7 phyla) of sessile organisms and 27 species (3 phyla) of nonsessile associates were recorded.Colonization by macrofouling organisms usually …
Effects Of Different Extenders, Cryoprotectants, Equilibration And Vapour Exposure On Freezability Of African Catfish(Clarias Gariepinus) Sperm.,
2010
Universiti Malaya
Effects Of Different Extenders, Cryoprotectants, Equilibration And Vapour Exposure On Freezability Of African Catfish(Clarias Gariepinus) Sperm., Noor Azlina Kamaruding
Student Works (2010-2019)
The aim of this study was to develop an optimal freezing protocol for African catfish(Clarias gariepinus) sperm with special reference to type of extender and cryoprotectant, molarity, equilibration duration, vapour temperature and vapour exposure duration. Using Tris-Citric Acid Yolk Extender (TCAYE), a 3x3x3x3 factorial experiment was carried out consisting of 3 molarities of glycerol (0.5, 1.0 and 2.0 M), 3 equilibration durations (120,140 and 160 minutes), 3 vapour temperatures (-80, -90 and -100oC) and 3 vapour exposure durations (5, 10 and 15 minutes). In addition, using Fish-Ringer Extender (FRE), a 3x3x3 factorial experiment was also conducted involving 3 equilibration durations …
A Preliminary Study Of The Freshwater Fauna Of Testate Amoebae Of Southeast Azerbaijan,
2010
TÜBİTAK
A Preliminary Study Of The Freshwater Fauna Of Testate Amoebae Of Southeast Azerbaijan, Nataly Snegovaya, Ilham Alekperov
Turkish Journal of Zoology
During 2005-2007, 75 species of the freshwater testate amoebae were found in the vicinities of Lenkoran (SE Azerbaijan). The morphology of 14 species is described. One genus and 3 species are new to science (Difflugia pisciformis sp.n., D. pseudoclaviformis sp.n., Lenkorania gen.n., Pseudodifflugia magna sp.n.). Eleven species are new for the fauna of the Caucasus and Azerbaijan.
Grandidierella Trispinosa, A New Species Of Amphipod From The Karachi Coast, Pakistan (Crustacea: Amphipoda: Aoridae),
2010
TÜBİTAK
Grandidierella Trispinosa, A New Species Of Amphipod From The Karachi Coast, Pakistan (Crustacea: Amphipoda: Aoridae), Hala Bano, Quddusi Bashir Kazmi
Turkish Journal of Zoology
Grandidierella trispinosa sp. nov., the first species of the genus Grandidierella Coutiére, 1904 from Pakistan, is described from specimens collected at Sandspit backwater, west of Karachi. The new species is illustrated and compared with allied species. The holotype is housed in the Marine Reference Collection and Resource Centre, University of Karachi.
Contributions To The Raphignathoid Fauna Of Turkey,With A Description Of A New Species Of Cryptognathus Kramer (Acari: Actinedida: Raphignathoidea), Mustafa Akyol, Kami̇l Koç
Turkish Journal of Zoology
A new species, Cryptognathus ayyildizi sp. nov. is described and illustrated. Raphignathus bathursti Meyer and Ryke, 1960 (Raphignathidae), and Cheylostigmaeus californicus Summers and Ehara, 1965 (Stigmaeidae) are redescribed, and are new records for the Turkish fauna. Their worldwide distribution is also given.
Faunistic Study Of The Aquatic Beetles (Coleoptera: Helophoridae) In The Bayburt, Giresun, Gümüşhane, Ordu, And Trabzon Provinces Of Turkey, Abdullah Mart, Ümi̇t İncekara, Hani̇fi̇ Karaca
Turkish Journal of Zoology
In this study, we established the presence of 25 aquatic beetle (Coleoptera: Helophoridae) species belonging to 5 subgenera from the Bayburt, Giresun, Gümüşhane, Ordu, and Trabzon provinces in the Black Sea region of Turkey. Specimens were collected between May 2007 and October 2007. Ten species are here recorded for the first time from the Black Sea region and 6 species are recorded for the first time in the research area. In addition, the habitats of these species found in the research area were given.
Lobster Attack Induces Sensitization In The Sea Hare, Aplysia Californica,
2010
Chapman University
Lobster Attack Induces Sensitization In The Sea Hare, Aplysia Californica, Amanda J. Watkins, Daniel A. Goldstein, Lucy C. Lee, Christina J. Pepino, Scott L. Tillett, Francis E. Ross, Elizabeth M. Wilder, Virginia A. Zachary, William G. Wright
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Studies of the neural mechanisms of learning, especially of sensitization, have benefitted from extensive research on the model species, Aplysia californica (hereafter Aplysia). Considering this volume of literature on mechanisms, it is surprising that our understanding of the ecological context of sensitization in Aplysia is completely lacking. Indeed, the widespread use of strong electric shock to induce sensitization (an enhancement of withdrawal reflexes following noxious stimulation) is completely unnatural and leaves unanswered the question of whether this simple form of learning has any ecological relevance. We hypothesized that sublethal attack by a co-occurring predator, the spiny lobster, Panulirus interruptus, might …
Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults,
2010
TÜBİTAK
Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults, Lati̇fe Beyaz, Ayhan Atasever, Fuat Aydin, K. Semi̇h Gümüşsoy, Seçi̇l Abay
Turkish Journal of Veterinary & Animal Sciences
This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 10^4, 1 × 10^6, and 1 × 10^9 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days …
Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons,
2010
TÜBİTAK
Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons, Mohammad Ali̇ Nilforooshan
Turkish Journal of Veterinary & Animal Sciences
Countries with different herd structures and climates use different combinations of herd, year, and season to define the effect of their climatic conditions and management. Estimates of variance components and Akaike information criterion values resulting from 8 analytical models differentiated by different combinations of herd, year and season effects were compared for milk and fat yields. Data consisted of pedigree and records of 11,850 Holsteins freshening in 38 dairy herds of Isfahan, Iran, between January 1994 and October 2001. Heritability estimates were lowest for the models that used herd-year combination as a random effect. Moreover, the use of these models …
The Effectiveness Of Grass Pea (Lathyrus Sativus L.) Seeds In Pig Feed,
2010
TÜBİTAK
The Effectiveness Of Grass Pea (Lathyrus Sativus L.) Seeds In Pig Feed, Anna Winiarska-Mieczan, Malgorzata Kwiecien
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to assess the usefulness of raw grass pea seeds in pig feed, and the usefulness of supplementing mixtures that contain raw grass pea seeds with a probiotic and an enzymatic preparation. The study included 96 fattening pigs (Polish Landrace × Polish Large White) × Pietrain that were divided into 4 feeding groups. Two experimental nutritional agents were used. The first was the source of protein in the diet: soybean meal and raw grass pea seeds (amounting to 50% of feed protein). The other experimental agent was mixtures containing raw grass pea seeds supplemented with …
The Effects Of L-Carnitine Administration On Energy Metabolism In Pregnant Halep (Damascus) Goats,
2010
TÜBİTAK
The Effects Of L-Carnitine Administration On Energy Metabolism In Pregnant Halep (Damascus) Goats, Ci̇han Kaçar, Abuzer Kaffar Zonturlu, Mahmut Karapehli̇van, Umut Çağri Ari, Meti̇n Öğün, Mehmet Çi̇ti̇l
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of parenteral administration of L-carnitine on some biochemical parameters in Halep (Damascus) goats during the last month of pregnancy. L-carnitine was administrated to goats in group I (n = 13) by subcutaneous injections once a week during the last month of the pregnancy. Physiologic salt solution was administered to goats in group II (n = 12) by the same route during the same period. Differences of glucose concentration between groups were not significant (P > 0.05). Serum \beta-hydroxybutyric acid (BHB) concentrations in both groups increased until parturition. However, differences between groups …
Immune Response To Type-2 Porcine Circovirus In Weaned Pigs,
2010
TÜBİTAK
Immune Response To Type-2 Porcine Circovirus In Weaned Pigs, Hong Chen, Ke-Ying Zhang, Xue-Mei Ding, Zhi-Wen Xu, Dai-Wen Chen, Gang Tian
Turkish Journal of Veterinary & Animal Sciences
Sixteen crossbred barrows (Duroc × Landrace × Yorkshire) weaned at 28 days with an initial body weight of 5.12-8.64 kg and no antibodies to PCV2 were used in a 5-week trial to evaluate the immune response after intranasal inoculation with type-2 porcine circovirus. The percentage of peripheral blood ANAE+ T cells, PCV2 antibody titer, immunoglobulins, and serum IFN-\gamma, IL-2, and sIL-2R levels were determined. The results show that the percentage of peripheral blood ANAE+ T cells was notably higher in inoculated pigs 5 days post-inoculation (dpi) and significantly lower 21 dpi than in the control group. The anti-PCV2 titer in …
Identification And Characterization Of Salmonella Isolates From Captured House Sparrows,
2010
TÜBİTAK
Identification And Characterization Of Salmonella Isolates From Captured House Sparrows, Sara Mirzaie, Mohammad Hassanzadeh, Iradj Ashrafi
Turkish Journal of Veterinary & Animal Sciences
The objectives of this study were to determine the prevalence of Salmonella in a population of house sparrows, which are commonly found around poultry houses, and to characterize the obtained Salmonella isolates via serotyping, multiplex polymerase chain reaction (PCR), and antibiotic resistance analysis. Samples of visceral organs (gastrointestinal tract, liver, and heart) from 470 house sparrows were subjected to culture and the results show that 18 (3.8%) were positive for Salmonella. Of the 18 Salmonella isolates characterized, the most predominant serovars were Salmonella Typhimurium and S. Enteritidis (9 and 8 cases each, respectively), whereas only 1 serovar belonged to S. …
The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis,
2010
TÜBİTAK
The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis, Umur Önal, İhsan Çeli̇k, Sebahatti̇n Ergün
Turkish Journal of Veterinary & Animal Sciences
The performance of a high-density continuous system was evaluated as an alternative to conventional low-density batch-culture systems for culturing the rotifer Brachionus plicatilis. Rotifer densities ranging from 1700 to 3760 individuals/mL were achieved in 4 different trials that lasted 10-18 days. Problems associated with NH_3-N and pH were controlled by increasing the water exchange rate and periodic addition of ammonia remover. While NH_3-N and pH values were 0-8.8 mg/L and 6.9-9.3, respectively, the most important problem was the accumulation of TSS at concentrations of up to 85 mL/L within a very short time after start-up. Further studies are required to …
Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat,
2010
TÜBİTAK
Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat, Giuseppe Piccione, Stefania Casella, Lucio Lutri, Irene Vazzana, Vincenzo Ferrantelli, Giovanni Caola
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine haematological, haematochemical, and electrophoretic reference values for the Girgentana goat in order to form a basis for clinical interpretation. The study included 348 female Girgentana goats aged from 1 to 6 years. The animals were divided into 3 groups: goats aged 1-2 years (group A), goats aged 3-4 years (group B), and goats aged 5-6 years (group C). Blood samples were collected from each animal every morning at the same time (0800) by means of jugular venipuncture, and red blood cell (RBC), white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM), monocyte (MONO), …
A Common Tortoise Tick, Hyalomma Aegyptium Linne 1758 (Acari: Ixodidae), Identified On Eastern Hedgehog (Erinaceus Concolor Martin 1838) In Central Anatolia, Serkal Gazyağci, Nursel Aşan, Yasi̇n Demi̇rbaş
Turkish Journal of Veterinary & Animal Sciences
Ticks are obligate blood sucker arthropods that infect animals and humans. A common tortoise tick, Hyalomma aegyptium, was collected from a young and an adult male hedgehog, Erinaceus concolor, from Central Anatolia in July 2008. More ticks were determined on the young one. This is the second record of tortoise tick that parasitizes a hedgehog.
Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha},
2010
TÜBİTAK
Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha}, Samir Z. El-Zarkouny
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to evaluate the effectiveness of GnRH PGF-based protocols on reproductive outcome in Holstein heifers. Heifers were randomly assigned to the control group (n = 14), or treatment groups (n = 50) treated with GnRH (day -7) and PGF_{2\alpha} (day 0). Heifers that exhibited estrus were inseminated (Select Synch, n = 34) and heifers that did not exhibit estrus up to 48 h after PGF_{2\alpha} injection received a second GnRH injection and were fixed-time inseminated (Ovsynch, n = 16). Conception rates were 40%, 55.9%, and 31.3%, and overall pregnancy rates (P < 0.05) were 64.3%, 97%, and 87.5% in the control, Select Synch, and Ovsynch groups, respectively. Ovsynch heifers had 1.8 ± 0.5 and 3.2 ± 0.6 ng mL^{–1} of serum P4, versus 3.3 ± 0.5 and 3.8 ± 0.6 ng mL–1 in the Select Synch heifers at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Conception rates were 55.6% and 58.3% when P4 concentrations were high, and were 50% and 25% when P4 concentrations were low at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Dairy heifers synchronized with GnRH and PGF_{2\alpha} should be inseminated when estrus is observed, and heifers that do not exhibit estrus up to 48 h after PGF_{2\alpha} injection should be given a second GnRH injection and fixed-time inseminated 16-20 h later.
