Dairy Science Commons^™

Assessing The Efficacy Of Automated Detection Of Estrus In Dairy Cattle, Lauren M. Mayo

Theses and Dissertations--Animal and Food Sciences

The detection of estrus continues to be a primary factor contributing to poor reproductive performance in modern dairy cattle. The objectives of this research were 1) to evaluate performance of automated detection of estrus using a reference standard of ovulation detection with temporal progesterone patterns 2) to evaluate the efficacy of parameters measured by automated detection of estrus systems 3) to evaluate the efficacy of alerts generated by several commercially available systems used for automated detection of estrus and 4) to determine the differences in these parameters among cows with or without poor health conditions at the time of estrus. …

Characterization Of Brd4 During Mammalian Post-Meiotic Sperm Development, Jessica M. Bryant, Greg Donahue, Xiaoshi Wang, Mirella Meyer-Ficca, Lacey J. Luense, Angela H. Weller, Marisa S. Bartolomei, Gerd A. Blobel, Ralph G. Meyer, Benjamin A. Garcia, Shelley L. Berger

Animal, Dairy, and Veterinary Science Faculty Publications

During spermiogenesis, the post-meiotic phase of mammalian spermatogenesis, transcription is progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin reorganization involving hyper-acetylation and replacement of most histones with protamines. Although BRDT functions in transcription and histone removal in spermatids, it is unknown whether other BET family proteins play a role. Immunofluorescence of spermatogenic cells revealed BRD4 in a ring around the nuclei of spermatids containing hyper-acetylated histones. The ring lies directly adjacent to the acroplaxome, the cytoskeletal base of the acrosome, previously linked to chromatin reorganization. The BRD4 ring does not form in acrosomal mutant mice. …

Examining Vegetative Growth Of Cool-Season Forage Grasses For Dairy Cattle Grazing Preference, Eric D. Billman

Theses and Dissertations--Plant and Soil Sciences

The objective of this study was to determine dairy cattle preference amongst four species of cool-season forage grasses: eight orchardgrasses (Dactylis glomerata L.), five tall fescues [Schedonorus arundinaceus (Schreb.) Dumort.], five perennial ryegrasses (Lolium perenne L.), and six festuloliums [xFestulolium braunii (K. Richt.) A. Camus.]; 24 cultivars in total. Each grazing trial utilized four Holstein-Friesian heifers over six hours. Maturity differences were eliminated by having animals graze only vegetative material. After six grazing trials (three each in 2014 and 2015), consistent results in animal preference were not found; three of the six trials did show preference …

Pathology In Practice - Fascioliasis In A Dahl Sheep, David J. Wilson, T. J. Baldwin, Kerry A. Rood

Animal, Dairy, and Veterinary Science Faculty Publications

In early fall 2012, a 48-kg (106-lb) sexually intact male Dahl ram in fair body condition was found dead; the owner observed no clinical signs prior to death. The ram was part of a flock of 50 game animals used for high-fence hunting that was moved among pastures in Idaho, Utah, and Montana. The ram was housed primarily on flood-irrigated pasture in common with approximately 170 captive Rocky Mountain elk (Cervus canadensis). The owner reported that 12 other sheep had died over the past year without observed clinical signs, but none were submitted for postmortem examination. Both sheep and elk …

Dairy Herd - Level Prevalence Of Johne's Disease And Bvd In The Intermountain West Of The U.S.A. And Farm Management Practices And Characteristics For Test-Positive Herds, David J. Wilson, Kerry A. Rood, C. Whitehouse, J. Bunnell, G. M. Goodell, T. M. Byrem

Animal, Dairy, and Veterinary Science Faculty Publications

Herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD) and Bovine Viral Diarrhea (BVD) virus were estimated on dairy farms in Utah. Duplicate milks were collected at 3-4 day intervals on 5 dates from each bulk tank on participating farms. Samples were tested at separate laboratories for BVD (real-time, RT-PCR) and for JD/MAP (ELISA and qPCR). 151/209 (72%) eligible dairy farms participated. Farms detected positive were: 58 JD (38%) and 14 BVD (9%); 5 farms had both diseases. Follow up visited farms’ (n=22) means, medians: 778,420 milking cows; 20,052 lbs, 20,311 lbs 305d milk; 175,545/ml, …

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …

The Effects Of Hmtba (2-Hydroxy-4-Methylthio-Butanoic Acid) Supplementation On Ruminal Microbial Crude Protein Synthesis And Community Structure In Dairy Cattle, Chad James Robert Jenkins

Department of Animal Science: Dissertations, Theses, and Student Research

Metabolizable protein (MP) is protein that reaches the small intestine and is available for absorption and utilization by the cow. Dairy rations may be limited in the supply of MP essential to meeting the demands of milk synthesis, however as much as half of the MP flowing to the small intestine may be attributed to microbial origins and is referred to as microbial CP (MCP). Experiment 1 utilized a technique in which DNA was used as a microbial marker to estimate the concentration of bacterial CP (BCP) in the solid and liquid portions of rumen digesta. Rumen digesta was sampled …

Efficient Gene Targeting In Golden Syrian Hamsters By The Crispr/Cas9 System, Zhiqiang Fan, Wei Li, Sang R. Lee, Qinggang Meng, Tom D. Bunch, Kenneth L. White, Zhongde Wang, Il-Keun Kong

Animal, Dairy, and Veterinary Science Faculty Publications

The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research. Here, we report the first successful application of the CRISPR/Cas9 system to efficiently conduct gene targeting in hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three for targeting the coding sequences for different functional domains of the hamster STAT2 protein, one for KCNQ1, and one for PPP1R12C—and demonstrated that the CRISPR/Cas9 system is highly efficient in introducing site-specific mutations in hamster somatic cells. We …

Effect Of Conditioned Medium On The Recovery Of Dormant Mycobacteria In Culture, Kelly E. Rock

Journal of Undergraduate Research at Minnesota State University, Mankato

Mycobacterium avium subsp. paratuberculosis (Mpt) is the etiologic agent of Johne’s disease, a chronic intestinal disease in cattle that threatens the economic viability of dairy farming. Diagnostic culture is typically unrewarding until several years after infection when clinical signs can be observed. This leads to widespread infection within the herd. Difficulty in culturing Mpt may be a result of oxidative damage due to the increased metabolic rate when dormant organisms are recovered in a nutrient rich medium. In order to improve recovery it is believed that some organisms secrete a growth factor in times of environmental stress which enables them …

Choline And Choline Metabolite Patterns And Associations In Blood And Milk During Lactation In Dairy Cows, Virginia M. Artegoitia, Jesse L. Foote, Federico M. Harte, Shawn R. Campagna, Michael J. De Veth

Department of Animal Science: Faculty Publications

Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in …

Energy Content Of Reduced-Fat Dried Distillers’ Grains And Solubles For Lactating Dairy Cows And Effects On Energy And Nitrogen Balance, Alison J. Foth

Department of Animal Science: Dissertations, Theses, and Student Research

Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat distillers grains and solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2 % corn silage, 18.4 % alfalfa hay, 6.94 % brome hay with either 22.9 % rolled corn and 14.8 % soybean meal (Control), or 8.95 % rolled corn, 28.8 % RFDDGS, and 0 % soybean meal (Co-P; DM basis). The inclusion of RFDDGS did not affect (P = 0.86) DMI averaging 21.4 ± 0.53 kg …

2013 Annual Report, Various Authors

Annual Reports

No abstract provided.

The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber

Animal Science

This study’s objectives were to determine if the biopsy of expanded blastocysts prior to cryopreservation affected the survival rates and determine if there is a difference between slow-freezing in 10% glycerol, slow freezing in ethylene glycol, and vitrification in terms of survival rate of the embryos. Blastocoeles were reduced prior to cryopreservation by means of biopsy. Embryos for slow freezing were exposed to ethylene glycol (SynGRO Ethylene Glycol with Sucrose, BIONICHE, Pullman, WA, USA) or 10% glycerol (SynGRO 10% Glycerol, BIONICHE, Pullman, WA, USA) as cryoprotectants. Vitrified embryos were exposed to 10% and 40% ethylene glycol. Survival rate of biopsied …

Ghrelin Concentrations In Milk And Plasma Of Dairy Cows During Early Lactation, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ghrelin is a hormone produced mainly by the cells lining the gastric mucosa. Ghrelin was first extracted from human and rat stomachs, and identified as an endogenous stimulator of growth hormone release. Ghrelin is synthesized and produced in several tissues, but the gastric mucosa remains the major source of circulating ghrelin. Besides growth hormone release, ghrelin stimulates appetite and plays some major roles in different organs. In several studies, ghrelin was described as a hormone with multiple functions and diverse biological actions. Ghrelin exists in two major forms, active ghrelin and inactive ghrelin, and only the active from binds to …

Physiological Impacts And Lactational Performance Of Dairy Cows Fed Brown Midrib Corn Silage During Dry Period Through Early To Midlactation, Alexandra Windley Kelley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Developing solutions to the metabolic stress experienced by cows during the transition period is very important because it can negatively influence lactational performance. The objectives were to: 1) compare physiological changes through body weight (BW) and concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) and 2) evaluate feed intake, milk production, and energy balance (EB) of cows fed brown midrib corn silage (BMRCS)-based diets when compared with conventional corn silage (CCS)-based diets during the transition. At 4 wk prior to parturition, 40 dry multiparous Holstein cows …

Methods Of Bovine Reproduction, Kelsey L. O'Donnell

Honors Scholar Theses

The beef and dairy cattle industries are of immense economic value in the United States and have made impressive advancements over the past century. Their success depends strongly upon the reproductive efficiency of their breeding programs. This overview discusses the major advancements in bovine reproduction that have been integrated into the beef and dairy cattle industries and also the emerging technologies that are currently being researched. Current practices like artificial insemination and estrus synchronization resulted from the development of a strong understanding of cattle physiology and the endocrinology of the estrous cycle. With these technologies, selection of superior genetic traits …

Tracking Heat-Resistant, Sporeforming Bacteria In The Milk Chain: A Farm To Table Approach, Maricarmen Estrada Anzueto

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Sporeforming bacteria (such as Bacillus and Paenibacillus spp.) can survive pasteurization conditions (Collins, 1981) and grow in pasteurized fluid milk during refrigerated storage (Huck et al., 2008; Ivy et al., 2012), causing fluid milk spoilage and limiting the further extension of fluid milk’s shelf life (Fromm and Boor, 2004; Durak et al., 2006). Moreover, Bacillus and related genera have been found in raw milk, pasteurized milk and environmental samples from dairy farms, indicating that these organisms are ubiquitous in nature and can enter the milk chain from different sources (Huck et al., 2007b; Huck et al., 2008; Ranieri and Boor, …

Halogeton (H. Glomeratus) Poisoning In Cattle: Case Report, Kerry A. Rood, Kip E. Panter, Dale R. Gardner, Bryan L. Stegelmeier, Jeffery O. Hall

Animal, Dairy, and Veterinary Science Faculty Publications

Historically, the most significant losses from halogeton poisoning have been reported in sheep, with multiple catastrophic deaths documented. While recorded death losses in cattle from halogeton poisoning are less common than in sheep, recent cases, including 2 reported here, and anecdotal reports from other ranchers suggest that the impact of halogeton losses in cattle herds in the western United States is much more widespread than originally thought. Halogeton may accumulate up to 30% oxalates; a small amount of the plant (300 g) is enough to cause death in sheep. Oxalates precipitate calcium from the blood, resulting in hypocalcemia, formation of …

P-28 The Prevalence Of Encysted Toxoplasma & Sarcocystis In Consumer-Grade Pork, Beef, And Mutton In Michiana, Ross Trecartin

Honors Scholars & Undergraduate Research Poster Symposium Programs

Commonly present in the muscle of animals are the tissue cysts of Toxoplasma and Sarcocystis. The aim of this study was to determine the presence and levels of Toxoplasma/Sarcocystis tissue cysts in retail pork, beef, and mutton throughout the Michiana area. A total of 36 samples, 12 from each species, were exposed to a digestive solution mimicking stomach conditions. The solution consisted of Pepsin 0.75% , NaCl 0.86%, and HCl adjusted to a pH of 1-2. The digested samples were then strained through several layers of cheesecloth, centrifuged, and examined for the presence of parasites.

Triple Immunoglobulin Gene Knockout Transchromosomic (Tc) Cattle: Bovine Lambda Cluster Deletion And Its Effect On Fully Human Polyclonal Antibody Production, H. Matsushita, A. Sano, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Zhongde Wang, K. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (hIGH), kappa-chain (hIGK), and lambda-chain (hIGL) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, were homozygously inactivated (bIGHM-/-, bIGHML1-/-; double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the hIGK and hIGL genomic loci on the HAC and from the endogenous …