Agriculture Commons^™

Control Of Erwinia Tracheiphila In Cucumis Melo, John R. Caudle

Theses and Dissertations--Plant and Soil Sciences

Currently there is no control of bacterial wilt disease, Erwinia tracheiphila, in susceptible cucurbit crops, once infection of the plant occurs. Conventional and organic production systems rely on insecticide applications to kill the vectors, striped and spotted cucumber beetles, Acalymma vittatum and Diabrotica undecimpunctata, respectively, prior to transmission of the pathogen which indirectly controls the disease to some extent. Physical barriers such as row covers are used to exclude the vectors from plants prior to flowering; however, pollination requirements expose plants to potential infection. Experimental field plots were developed to test various enhanced organic production systems in an …

Nitrogen–Climate Interactions In Us Agriculture, G. Philip Robertson, Tom W. Bruulsema, Ron J. Gehl, David Kanter, Denise L. Mauzerall, C. Alan Rotz, Candiss O. Williams

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Agriculture in the United States (US) cycles large quantities of nitrogen (N) to produce food, fuel, and fiber and is a major source of excess reactive nitrogen (Nr) in the environment. Nitrogen lost from cropping systems and animal operations moves to waterways, groundwater, and the atmosphere. Changes in climate and climate variability may further affect the ability of agricultural systems to conserve N. The N that escapes affects climate directly through the emissions of nitrous oxide (N₂O), and indirectly through the loss of nitrate (NO₃^-), nitrogen oxides (NO_x) and ammonia to downstream and …

How Does A Mycobacterium Change Its Spots? Applying Molecular Tools To Track Diverse Strains Of Mycobacterium Avium Subspecies Paratuberculosis, John Bannantine, L.-L. Li, Srinand Sreevatsan, Vivek Kapur

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Defining genetic diversity in the wake of the release of several Mycobacterium avium subsp. paratuberculosis (MAP) genome sequences has become a major emphasis in the molecular biology and epidemiology of Johne’s disease research. These data can now be used to define the extent of strain diversity on the farm. However, to perform these important tasks, researchers must have a way to distinguish the many MAP isolates/strains that are present in the environment or host to enable tracking over time. Recent studies have described genetic diversity of the Mycobacterium avium complex (MAC), of which MAP is a member, through pulsed-field gel …

Interferon Gamma Responses To Proteome-Determined Specific Recombinant Proteins: Potential As Diagnostic Markers For Ovine Johne’S Disease, Valerie Hughes, Susan Denham, J. P. Bannantine, Francesca Chianini, Karen Kerr, Linda May, Joyce Mcluckie, Mintu Nath, Karen Stevenson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Johne’s disease (JD), or paratuberculosis is a fatal enteritis of animals caused by infection with Mycobacterium avium subspecies paratuberculosis (Map). There may be a long subclinical phase with no signs of clinical disease.

Diagnosis of JD is problematic and no test can reliably detect sub-clinical disease. Th1 responses to Map are believed to be activated first with a later switch to Th2 responses and progression to clinical disease. Detection of a cell-mediated response, indicated by interferon gamma (IFN-􏰁) produced in response to mycobacterial antigens, may give an early indication of infection. Crude extracts of Map (PPDj) have been used to …

Growing South Dakota (Winter 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2012 South Dakota Agricultural Station Annual Report

CONTENTS:
[Page] 1 From The Dean
[Page] 2 Commitment To Collaboration: Working Together Adds Efficiency To Budgets: Effectiveness To Solutions
[Page] 7 Special Pull-Out Section: South Dakota Agricultural Experiment Station Annual Report: Highlighting Research Projects On Beef; Wheat; And Soybean Meal For Aquaculture
[Page] 11 Water Watchers: The South Dakota Water Resources Institute Provides Research, Education & Outreach
[Page] 12 Winter College News
[Page] 14 SDSU Extension: One Year Later: Reorganization Prompted Restructuring – And Renewed Commitment
[Page] 16 Bullseye!: South Dakota’s 4-H Shooting Sports Program Sees Continued Growth …

Development And Evaluation Of Soysnp50k, A High-Density Genotyping Array For Soybean, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The objective of this research was to identify single nucleotide polymorphisms (SNPs) and to develop an Illumina Infinium BeadChip that contained over 50,000 SNPs from soybean (Glycine max L. Merr.). A total of 498,921,777 reads 35–45 bp in length were obtained from DNA sequence analysis of reduced representation libraries from several soybean accessions which included six cultivated and two wild soybean (G. soja Sieb. et Zucc.) genotypes. These reads were mapped to the soybean whole genome sequence and 209,903 SNPs were identified. After applying several filters, a total of 146,161 of the 209,903 SNPs were determined to be …

Mapping Soybean Aphid Resistance Genes In Pi 567598b, Carmille Bales, Guorong Zhang, Menghan Liu, Clarice Mensah, Cuihua Gu, Qijian Song, D. L. Hyten, P. B. Cregan, Dechun Wang

Department of Agronomy and Horticulture: Faculty Publications

The soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America since it was first reported in 2000. Our previous study revealed that the strong aphid resistance of plant introduction (PI) 567598B was controlled by two recessive genes. The objective of this study was to locate these two genes on the soybean genetic linkage map using molecular markers. A mapping population of 282 F_4:5 lines derived from IA2070 X E06902 was evaluated for aphid resistance in a field trial in 2009 and a greenhouse trial in 2010. Two …

Understanding And Disrupting Institutional Settings : Using Networks Of Conversations To Re-Imagine Future Farming Lives, Robert Campbell

Theses: Doctorates and Masters

Farmers in Australia and elsewhere face the challenge of remaining profitable whilst dealing with adverse structural arrangements and public expectations to better manage environmental degradation. This thesis draws on arguments that dominant paradigms in agricultural science and environmental management have often been ineffective in addressing these apparently competing demands and appear poorly suited to ‘messy’ situations characterized by uncertainty and complexity, and in which diverse stakeholders are motivated by varying goals and values. Engaging with such situations requires a philosophy and methodology that accepts a multiplicity of perspectives and which seeks to learn about and reflect upon novel ways of …

Barrow Island Ute Guide - Non-Indigenous Invertebrates, Frogs, Reptiles & Weeds, Mike Grimm, Kristofer Collett, Jessica Harrison, Peter Davis, Marc Widmer, Andras Szito, Rob Emery, Peter Mangano, John Botha, David Robinson, Brad Maryan

Biosecurity published reports

Barrow Island, the second largest island in Western Australia, is a Class A Nature Reserve and home to a variety of rare and endangered fauna and flora, some of which occur nowhere else. To protect fauna and flora it is essential workers and visitors to the island are aware of the risks that a non-indigenous species (NIS) poses to the ecological balance of Barrow Island.

Workers and visitors to Barrow Island need to be aware and keep their eyes open for any unusual fauna and flora that might be in accommodation units, recreation areas or the work place. Reports from …

Low Root Zone Temperature Limits Nutrient Effects On Cucumber Seedling Growth And Induces Adversity Physiological Response, Qiu-Yan Yan, Zeng-Qiang Duan, Jing-Dong Mao, Xun Li, Fei Dong

Chemistry & Biochemistry Faculty Publications

Effects of root-zone temperatures (RZT) (12°C-RZT and 20°C-RZT) and different N, P, and K nutrient regimes on the growth, reactive oxygen species (ROS), and antioxidant enzyme in cucumber seedlings were investigated in hydroponics. Strong interactions were observed between RZT and nutrient on the dry weight (P=0.001), root length (P=0.001) and leaf area (P=0.05). Plant dry weights were suppressed at low RZT of 12°C, while higher biomass and growth of cucumber seedlings were produced at elevated RZT of 20°C under each nutrient treatment. Growth indexes (plant height, internode length, root length, and leaf area) at 12°C-RZT had less difference among nutrient …

Seroprevalence Of Brucellosis In Livestock Within Three Endemic Regions Of The Country Of Georgia, Eliso Mamisashvili, Ian T. Kracalik, Tinatin Onashvili, Lela Kerdzevadze, Ketevan Goginashvili, Tamar Tigilauri, Marina Donduashvili, Marina Nikolaishvili, Irma Beradze, Marina Zakareishvili, Maka Kokhreidze, Makvala Gelashvili, Nino Vepkhvadze, S. Elizabeth Rácz, Philip H. Elzer, Mikeljon P. Nikolich, Jason K. Blackburn

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Brucellosis is the one of most common livestock zoonoses in Georgia, resulting in significant economic losses. Livestock were sampled in three regions of Georgia (Kakheti, Kvemo Kartli, Imereti). Districts that historically reported high numbers of brucellosis related morbidity were selected for serological, bacteriological and molecular surveys. Surveying efforts yielded samples from 10,819 large and small ruminants. In total, 735 serological tests were positive on Rose Bengal and 33 bacterial isolates were recovered and identified as Brucella melitensis or Brucella abortus by microbiology and AMOS-PCR. A Bayesian framework was implemented to estimate the true prevalence of the disease given an imperfect …

Insecticidal Activity Of Essential Oil Of Prangos Ferulacea (Umbelliferae) Against Ephestia Kuehniella (Lepidoptera: Pyralidae) And Trichogramma Embryophagum (Hymenoptera: Trichogrammatidae), Fahri̇ye Ercan, Hati̇ce Baş, Murat Koç, Di̇lek Pandir, Sevcan Öztemi̇z

Turkish Journal of Agriculture and Forestry

Essential oil vapors obtained by the hydrodistillation of Prangos ferulacea (Umbelliferae) were tested on the different stages of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) and egg parasitoid Trichogramma embryophagum Hartig (Hymenoptera: Trichogrammatidae). Extracts of the volatile fractions from P. ferulacea were analyzed by capillary gas chromatography-mass spectrometry. The major compound of the essential oil was detected as 2,3,6-trimethyl benzaldehyde (66.59%) and the minor compound was heneicosane (0.02%). The third instar larvae of E. kuehniella (LC_{50}: 379.662 µL L^{-1} air and LC_{99}: 538.755 µL L^{-1} air) and the pupal stage of T. embryophagum (LC_{50}: 5.947 µL L^{-1} air and LC_{99}: 19.568 µL …

Rice Cultures And Nitrogen Rate Effects On Yield And Quality Of Rice (Oryza Sativa L.), Muhammad Maqsood, Muhammad Asif Shehzad, Syed N. Azam Ali, Munawar Iqbal

Turkish Journal of Agriculture and Forestry

Field studies in 2010 and 2011 were conducted to evaluate the effect of variable nitrogen rates (0-100 kg N ha^{-1}) and culture methods (transplanted and direct) on chlorophyll contents, yield, grain quality, and economics in rice crop (Oryza sativa L. 'Basmati-385'). Transplanted rice gave a significantly higher paddy yield of 19.18% versus direct-seeded crop. Nitrogen application at 100 kg N ha^{-1} provided a maximum paddy yield (4.39 and 4.67 t ha^{-1}) in both years. The rice kernel characteristics were also affected by culture methods and nitrogen levels. In addition, return variable cost in the transplanted rice increased by 22.27% over …

Evaluation Of Efficiency Parameters Of Phosphorous-Solubilizing And N-Fixing Bacteria Inoculations In Wheat (Triticum Aestivum L.), Sancar Bulut

Turkish Journal of Agriculture and Forestry

Chemical fertilizers play a significant role in increasing plant production; however, they may have a negative impact on soil fertility and cause environmental degradation in cases of excessive use. In addition, the highest levels in chemical fertilizer use have already been reached, and further yield increases do not seem possible. Due to their negative impact on soils and the environment, alternatives to chemical fertilizers are needed. Today, biological alternatives are yielding promising outcomes. Biological nitrogen fixation may constitute a significant alternative in organic farming. Therefore, in the present study, the effects of single, dual, and triple combinations of phosphorus-solubilizing [Bacillus …

Concept Design In Virtual Reality Of A Forestry Trailer Using A Qfd-Triz Based Approach, Kenan Melemez, Giuseppe Di Gironimo, Gianpiero Esposito, Antonio Lanzotti

Turkish Journal of Agriculture and Forestry

Trailers are used to carry logs having specific dimensions for logging extraction operations, secondary transportation, and agricultural activities. The main objective of this study was the design of a timber trailer capable of manual loading operations and suitable for rough terrain conditions to be used in combination with a farm tractor in the western Black Sea region of Turkey. In the proposed methodology, a House of Quality diagram was used to transfer customer requirements into quality characteristics, and focus the development of the product toward customer satisfaction. Negative correlations between quality characteristics were solved using the TRIZ contradiction toolkit and …

Extraction Of Phenolic Compounds From Melissa Using Microwave And Ultrasound, Alev Emi̇ne İnce, Serpi̇l Şahi̇n, Servet Gülüm Şümnü

Turkish Journal of Agriculture and Forestry

In this study, extraction of phenolic compounds from melissa by microwave and ultrasound was studied. In both the microwave and ultrasound extractions, the effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid-to-solvent ratio (1:10, 1:20, and 1:30 g mL^{-1}) on total phenolic content (TPC) were investigated. Effects of different powers (50% and 80%) were also studied for the ultrasound extraction. As a solvent, water was used. In microwave extractions, the highest TPC of extracts (145.8 mg GAE g^{-1} dry material) was obtained in 5 min and at a 1:30 solid-to-solvent ratio. For ultrasound extraction, the …

Changes In Stem Growth Rates And Root Wood Anatomy Of Oriental Beech After A Landslide Event In Hanyeri, Bartın, Turkey, Barbaros Yaman, Nesi̇be Köse, Ünal Akkemi̇k

Turkish Journal of Agriculture and Forestry

On 19 May 1998, a small-scale landslide occurred near Hanyeri, Bartın, in the western Black Sea region. The purpose of the study is to investigate the changes in stem growth rates and root wood anatomy of oriental beech (Fagus orientalis Lipsky). According to results from 8 years before and after the event year, growth variation in the affected trees is significantly high. In the control trees, growth increases after the event year, and the growth variation is 116.22%; this value is 69.59% in the affected trees. However, the growth variation values for ±11 years are 107.69% and 81.36% in control …

The Effect Of Tillage Systems On Yield And Quality Of Durum Wheat Cultivars, Andrzej Wozniak

Turkish Journal of Agriculture and Forestry

A field experiment was conducted for 3 years, from 2008 to 2010, in Uhrusk, Poland, to investigate the effect of tillage systems on the yield and quality of durum wheat cultivars Floradur and Lloyd. The 3 tillage systems were: 1) conventional tillage (CT) - shallow ploughing and harrowing after harvest of the previous crop, ploughing in the autumn; 2) reduced tillage (RT) - only a cultivator was used after harvest of the previous crop; and 3) herbicide tillage (HT) - only Roundup 360 SL was used after harvest of the previous crop. Both cultivars were found to respond differently to …

Chitosan Coating Improves The Shelf Life And Postharvest Quality Of Table Grape (Vitis Vinifera) Cultivar Shahroudi, Mohammad Ali Shiri, Davood Bakhshi, Mahmood Ghasemnezhad, Monad Dadi, Alexandros Papachatzis, Helen Kalorizou

Turkish Journal of Agriculture and Forestry

Table grapes (Vitis vinifera) of the cultivar Shahroudi were coated with 0.5% or 1% chitosan and then stored at 0 °C for 60 days. Shelf-life qualitative traits including rachis and berry appearance and berry cracking, shattering, decay, browning, acceptability, and flavor were evaluated. Treated berries showed less weight loss, decay, browning, shattering, and cracking. Flavor-related factors such as total soluble solids (TSS), titratable acidity (TA), and TSS/TA ratio levels were higher in treated fruits; however, there was no significant difference between the fruits treated with 0.5% and 1% chitosan. Changes of the total phenolics, catechin, and antioxidant capacity of the …

An Efficient Multiplex Pcr Assay For Early Detection Of Agrobacterium Tumefaciens In Transgenic Plant Materials, Li Yang, Changchun Wang, Lihuan Wang, Changjie Xu, Kunsong Chen

Turkish Journal of Agriculture and Forestry

The multiplex polymerase chain reaction (MPCR) is a variant of conventional PCR in which 2 or more target sequences are amplified by more than 1 pair of primers in a single reaction mixture. In this study, an efficient MPCR assay was established and optimized for the quick detection of Agrobacterium-derived plant transformants. The neomycin phosphotransferase II gene fragment (nptII; target gene) and the chromosomal virulence gene ChvA fragment of Agrobacterium tumefaciens (external reference gene) were amplified simultaneously with 2 pairs of corresponding primers. The MPCR results showed that 9 out of 10 kanamycin-resistant lines tested were transformed with the foreign …