Large or Food Animal and Equine Medicine Commons^™

Supplemental Lysine In Finishing Cattle Diets, Hanna Cronk, Zac Carlson, Mitch Norman, Levi Mcphillips, Andrea Watson, Galen E. Erickson, Gary Ducharme

Nebraska Beef Cattle Reports

The objective of this experiment was to evaluate the effects of increasing lysine supply in finishing beef cattle diets. Crossbreed steers (n=120, BW=577 ± 2 lb) were individually fed using a Calen Gate system for 195 days. Animals received a common finishing diet (63% corn, 15% corn silage and 15% distillers grains) with 0, 1, 2, 3, 4, 5, 6, or 7 g per day of supplemental rumen bypass lysine. High levels of lysine (5 or more g per day) in the diet decreased dry matter intake, carcass adjusted body weight with no impact on carcass adjusted feed efficiency. Hot …

Impact Of Removing 20% Distillers Grain After One-Third Or Two-Thirds Of The Feeding Period On Performance Of Finishing Yearling, Sofia Suarez Lorences, Braden C. Troyer, Mitch M. Norman, Pablo L. Loza, Rick Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study evaluated the effect of removing modified distillers grains plus solubles after one-third or two-thirds of the feeding period on performance and carcass characteristics of yearling steers. Treatment diets included 20% modified distillers for the entire feeding period; 20% modified distillers for two-thirds of the feeding period and then 0%; and 20% modified distillers for one-third of the feeding period, then 0%. Removing modified distillers from the diet decreased average daily gain, final body weight and dry matter intake and tended to increase feed conversion. Cattle fed 20% modified distillers throughout the entire feeding period had greatest hot …

Impact Of Constant Inclusion Or Decreasing Inclusion Of Distillers Grains With High-Quality Or Low-Quality Roughage On Finishing Cattle Performance, Sofia Suarez Lorences, University Of Nebraska - Lincoln, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing trial was conducted to evaluate high-quality or low-quality roughage inclusion in diets containing three concentrations of modified distillers grains plus solubles on finishing cattle performance. In a randomized block design, steers were fed according to a 2x3 factorial arrangement with two roughage sources (6% corn stalks versus 12% corn silage) in three diets containing 0, 15, or decreasing inclusion of distillers (30, 15, then 0% across the feeding period). No interactions were observed between distillers inclusion and roughage source except for intake. Steers fed corn silage consumed less, gained the same, and had slightly better feed conversions Steers …

Evaluate The Effect Of Corn Processing, Drying Distillers Grains, Oil Removal From Distillers Grains, And Distillers Inclusion On Cattle Performance, Braden C. Troyer, Elliott James Dennis, Alfredo Dicastanzo, Galen E. Erickson

Nebraska Beef Cattle Reports

An analysis of over 9,300 head of cattle and 980 pen means was conducted to evaluate the effect of corn processing, drying distillers grains, oil removal from distillers, and distillers inclusion on cattle performance. This analysis looked at both steam-flaked corn and high-moisture corn or dry-rolled corn or a blend of the latter two grains and their effects on performance with and without distillers grains. Additionally, wet, modified, and dried distillers grains were analyzed as both full fat or de-oiled products at various dietary concentrations with each corn type as the primary cereal grain to determine performance responses. There was …

Effects Of Individual Sweet Bran Components In Beef Finishing Diets On Nutrient Digestion, Rebecca L. Sjostrand, Rittikeard Prachumchai, Maggie Youngers, Rick A. Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Sweet Bran is a branded wet corn gluten feed recognized for improving rumen health, energy intake, and gains in finishing cattle. Eight ruminally cannulated steers were utilized in a replicated 4 x 4 Latin Square design to evaluate the effect of individual Sweet Bran components on total tract digestibility and rumen fermentation parameters. Three Sweet Bran components (solvent extracted germ meal, corn bran, and mixed steep) were included at 40% of diet dry matter in their respective treatment, with a steam-flaked corn control diet. Total tract dry matter and organic matter digestibility were least for bran, intermediate for solvent extracted …

Evaluation Of Lactiproflx In An Acidosis Challenge Model, Samantha K. Wagner, Rebecca J. Sjostrand, Tyler J. Spore, Mark E. Corrigan, Galen E. Erickson, J. C. Macdonald

Nebraska Beef Cattle Reports

An acidosis challenge study was conducted comparing different administration techniques for LactiproFLX, a direct fed microbial product containing Megaspharea elsdenii (a lactate-utilizing bacteria) for the prevention of acidosis. Four treatments were utilized in a randomized block design with 24 ruminally cannulated steers. Treatments consisted of a control group which did not receive the product, a group which received the commercial dose of the product four days before the acidosis challenge, one which received the commercial dose of the product one day before the challenge, and one which received ten times the commercial dose one day before the challenge. No differences …

Evaluation Of Encapsulated Megasphaera Elsdenii In An Accelerated Beef Step-Up Program And An Acidosis Challenge Event, Cindy D. Mansfield, Rebecca L. Sjostrand, Braden C. Troyer, Mitchell M. Norman, Tyler Spore, Mark Corrigan, Galen E. Erickson, J. C. Macdonald

Nebraska Beef Cattle Reports

A 100-day metabolism study with 40 ruminally cannulated steers, individually fed, was conducted to determine the efects of daily feeding of encapsulated Megasphaera elsdenii along with a one-time dose of Lactipro NXT on dry matter intake, rumen organic acid concentration, lactate disappearance and native and specific strains of Megasphaera elsdenii concentration following an acidosis challenge. Steers fed Megasphaera elsdenii daily had greater intake after an acidosis event. Cattle fed daily Megasphaera elsdenii also had a faster rate of lactic acid disappearance after an acidosis event. Feeding Megasphaeara elsdenii daily may result in a faster recovery time, after an acidosis event, …

Effect Of Ad Libitum Vs. Limit Feeding Program At Receiving On Morbidity And Performance Of Feedlot Calves, Rebecca A. Funk, Braden C. Troyer, Levi J. Mcphillips, Mitchell M. Norman, Galen E. Erickson

Nebraska Beef Cattle Reports

A two-phase study was conducted to determine the effects of two different feed delivery strategies during the receiving period of feedlot calves. Calves were fed either by limit-feeding at approximately 75% of ad libitum, or ad libitum feed offerings for the 30-d receiving period to determine effects on health and performance. During the receiving period, average daily gain and total weight gained was increased for the ad libitum treatment. No differences between ad libitum and limit-fed treatment groups were observed in either feed to gain or morbidity rates. During the second phase of the trial, a subset of calves was …

Effect Of Species And Maturity On Small Grain Silage Yield And Quality, Abigail M. Sartin, Kallie J. Calus, Morgan T. Grabau, Alyssa K. Kuhn, Mary E. Drewnoski, D. D. Redfearn

Nebraska Beef Cattle Reports

A study was conducted to determine the effect of species and maturity on yield and nutritive value of winter-hardy small cereal grains used for silage. Three species were evaluated: cereal rye, winter triticale, and winter wheat at four different stages of maturity: boot, pollination, milk, and soft dough. As species matured, yield increased across all stages, but crude protein (CP) and digestible organic matter (DOM) decreased, except at soft dough where there was a slight increase in DOM. Crude protein was greatest at the boot stage at 17.7% and least at soft dough at 9.8%. When comparing species, rye and …

Evaluation Of Ankom F58 Filter Bags Compared To Beakers For Analysis Of Neutral Detergent Fiber, Sawyer R. Kappel, Rebecca L. Sjostrand, J. C. Macdonald

Nebraska Beef Cattle Reports

Feed ingredient, feed refusals, duodenal and fecal samples were analyzed to compare two methods of determining neutral detergent fiber. All samples were weighed into Ankom F58 fiber bags and Analyzed using an Ankom 2000 automated fiber Analyzer. Results were then compared to the Van Soest beaker method. The fiber values determined from both methods were within 3.5% of one another, with the beaker method being consistently greater compared to the Ankom method, except for fecal samples. Variability in fiber estimates for ingredients, feed refusals, and feces translated to substantial inconsistency in estimated neutral detergent fiber digestibilities among treatments. It is …

Forage Evaluation Of Crested Wheatgrass, Kelton C. Adair, K. H. Jenkins Pas, Andrea K. Watson, James C. Macdonald

Nebraska Beef Cattle Reports

An experiment evaluated the forage value of crested wheatgrass (CWG) harvested throughout the grazing season near Sidney, Nebraska over a two-year period (2019-2020). The purpose of the evaluation was to determine forage quality and rumen undegradable protein (RUP) content to help producers with supplementation decisions for cattle grazing monoculture CWG pastures. In vitro dry matter disappearance quadratically decreased from 54% in May to 37% in September of 2019, with no changes throughout 2020, averaging 43%. In both years, crude protein (CP) decreased throughout the growing season while rumen undegradable protein (RUP) increased (as % CP). Digestible RUP was less than …

Effects Of Strategic Supplementation On Return To Management And Performance Of Yearling Cattle, Kaylee E. Wheeler, Dean Dustin, Jay Parsons, M. E. Drewnoski, K. H. Jenkins Pas

Nebraska Beef Cattle Reports

A three-year experiment evaluated the effects of two supplementation strategies on yearling cattle performance and producer returns to management. Yearling cattle grazed on crested wheatgrass pastures and were supplemented either throughout the entire grazing season, only during the latter part of the grazing season, or not supplemented at all. The supplemented yearlings received 3.5 lb of dried distillers grains with solubles 6 days/week. On average non-supplemented yearlings had an average daily gain of 1.1 lb/d and providing supplement increased average daily gain by 0.5 lb/d. Yearlings supplemented during the latter part of the season had similar performance to yearlings supplemented …

Timing Of Implant Use In The Backgrounding System, Kelton C. Adair, Kylie M. Butterfield, Zach E. Carlson, Braden C. Troyer, Jiehua Xiong, Mitchell M. Norman, James C. Macdonald, Galen E. Erickson, M. E. Drewnoski, Brandon L. Nuttelman

Nebraska Beef Cattle Reports

A two-year study utilized 240 weaned steers each year in a 3-phase yearling production system: winter backgrounding, summer grazing, and finishing. The objective of the study was to determine the effects of winter rate of gain (LOW or HIGH) and implant strategy during the winter backgrounding and summer grazing periods on compensatory gain, animal performance, and carcass characteristics. Calves received 1 of 3 implant strategies: NONE, SINGLE (Revalor-G during summer grazing), and MULTIPLE (Ralgro during winter backgrounding and Revalor-G during summer grazing). All cattle received a Revalor-XS during the finishing phase. Implant strategy and rate of gain during the winter …

Artificial Insemination Of Beef Heifers With Multi-Sire Sexed Semen, Dempster Christenson, John Maddux, Jordan M. Thomas, R. Funston

Nebraska Beef Cattle Reports

This study observed pregnancy outcomes resulting from artificial insemination of beef heifers with multi-sire sexed semen. It was hypothesized, pregnancy rates resulting from multi-sire sexed semen would be increased compared to pregnancy rates from prior studies using single-sire sexed semen. Producers looking to maximize the proportion of calves of one sex may consider the technique. Ultrasound determined pregnancy rate of heifers given multi-sire sexed semen was 65%, which is 12% greater than the average pregnancy rate reported in previous studies using single-sire sexed semen. After accounting for date of birth however, the adjusted AI conception rate was calculated to range …

Extending Melengestrol Acetate Treatment From Fourteen To Eighteen Days In Beef Heifers, Dempster Christenson, Jordan M. Thomas, Daniel J. Kelly, R. Funston

Nebraska Beef Cattle Reports

This study compared estrus synchronization, estrus response, and artificial insemination pregnancy rates of beef heifers fed melengestrol acetate the normal 14-day period or extended to an 18-day period using the melengestrol acetate -prostaglandin- heat detect and timed artificial insemination protocol. Therefore, the purpose of this study was to evaluate if extending melengestrol acetate feeding by 4 days increases the number of heifers ovulating, which would result in greater estrus response and pregnancy rate in the whole herd. Early estrus response following prostaglandin administration occurred in heifers who averaged an earlier estrus response after melegestrol acetate withdrawal but extending the melengestrol …

Effect Of Increasing Energy 30 Days Prior To Artificial Insemination In Beef Heifers, Landon F. Tadich, Rachel E.S. Rogers, Jim R. Teichert, R. Funston

Nebraska Beef Cattle Reports

A 3-yr study utilized 300 Angus-based, spring-born heifers to evaluate postweaning heifer development systems on gain and reproductive performance. Three groups of heifers were developed over the winter development period either grazing winter range or fed a dry lot diet targeted to 1.5 lb/d of gain in order to achieve 65% of their mature body weight at breeding. Thirty days prior to artificial insemination, heifers grazing winter rand entered the dry lot and were fed this same diet, one group of dry lot heifers remained on this diet, and the other received increased energy in the form of wet corn …

Including Gene Edited Sires In Genetic Evaluations, Leticia P. Sanglard, Garret M. See, Matthew L. Spangler

Nebraska Beef Cattle Reports

A simulation study investigated and provided potential solutions to practical issues that could arise from including gene-edited sires in routine genetic evaluations. Gene-editing is a technique for adding, deleting, or replacing nucleotides in the genome. Editing nucleotides controlling important socioeconomic traits (e.g., growth, carcass, disease susceptibility) is expected to improve rates of genetic gain. However, targeted alterations of the genome can affect the relationship among individuals and, consequently, introduce bias in Expected Progeny Differences. The current study illustrated that, indeed, Expected Progeny Differences for the progeny of edited sires were underestimated. Consequently, these animals would be less likely to be …

Comparison Of Semi-Confined And Pasture-Based August Calving Beef Cow Systems, Hannah F. Speer, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

Limited pasture availability and increased pasture rental rates have generated a need to evaluate alternative cow-calf production systems. The current study compared cow and calf performance in two August-calving cow systems that combined corn residue grazing with 1) perennial forage grazing and hay or 2) summer drylot feeding and fall cover crop grazing. Differences in pregnancy rates between systems within year were not observed; however, the effect of production system on cow body condition and calf body weight at different time points varied across years. Overall, cow and calf performance were not negatively impacted in the drylot/cropland system, suggesting that …

Impact Of Planning Horizon Length On Breeding Objectives And Resulting Selection Decisions, Hunter F. Valasek, Bruce L. Golden, Matthew L. Spangler

Nebraska Beef Cattle Reports

The weighting of traits in a selection index depends on varying circumstances that can difer among beef producers (e.g. economic factors, breeding systems). The determination of planning horizon is an additional variable that can differ among producers that represents the impact of genetic selection decisions over a gradient of time. A web-based economic index construction platform (iGENDEC) was used in the current study to investigate the implications of planning horizon on relative emphasis values of traits within the breeding goal and potential differences in sire selection decisions. General-purpose indexes were created for three breeding systems under six different …

A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyag Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu

Computer Science Faculty Publications

Background: Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.

Methods: Skeletal muscle single-cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/Brahman cross. Sophisticated bioinformatics analyses, including clustering analysis, gene set enrichment analyses, gene regulatory network construction, RNA velocity, pseudotime analysis, and cell-cell communication analysis, were performed to elucidate …

Effects Of High-Speed Training On Messenger Rna Expression In Two-Year-Old Thoroughbred Racehorses, Mackenzie Grace Johnson

Theses and Dissertations--Veterinary Science

Accumulating high-speed exercise has been identified as a significant risk factor for catastrophic injuries in racing Thoroughbreds. Injuries, regardless of severity, are a main cause of withdrawal from the racing industry, raising animal welfare concerns and resulting in significant economic losses. While most of the current literature focuses on catastrophic injuries incurred during racing rather than training, the present study aims to help fill this gap as well as discuss the associated risk factors. The evaluation of messenger RNA (mRNA) expression changes provides an efficient and straightforward approach to identifying horses at risk for catastrophic injury. While alternative injury risk …

Equine Fecal Microbiota Response To Short Term Antibiotic Administration, Jordan Lacey Parker

Theses and Dissertations--Veterinary Science

Though generally regarded as safe, research continues to demonstrate negative side effects of antibiotic administration on the gastrointestinal (GIT) microbiota across species. In horses, antibiotic associated diarrhea (AAD) is a life-threatening side effect linked to the GIT microbiota. This study tested the hypothesis that short term antibiotic administration to healthy horses would negatively impact the fecal microbiota as measured by their ability to digest nutrients and produce short chain fatty acids (SCFAs). Twenty-four horses were assigned to one of four treatments: control (CO); potassium penicillin/gentamicin sulfate (KPG); ceftiofur crystalline free acid (EX); trimethoprim/sulfamethoxazole (SMZ); and treated for 4 days. Fecal …

First Bovine Vaccine To Prevent Human Schistosomiasis - A Cluster Randomised Phase 3 Clinical Trial, Allen G. Ross, Donald A. Harn, Delia Chy, Marianette Inobaya, Jerric R. Guevarra, Lisa Shollenberger, Yuesheng Li, Donald P. Mcmanus, Darren J. Gray, Gail M. Williams

Biological Sciences Faculty Publications

Objective

Schistosomiasis is a neglected tropical parasitic disease caused by blood flukes of the genus Schistosoma. Schistosoma japonicum is zoonotic in China, the Philippines, and Indonesia, with bovines acting as major reservoirs of human infection. The primary objective of the trial was to examine the impact of a combination of human mass chemotherapy, snail control through mollusciciding, and SjCTPI bovine vaccination on the rate of human infection.

Methods

A 5-year phase IIIa cluster randomized control trial was conducted among 18 schistosomiasis-endemic villages comprising 18,221 residents in Northern Samar, The Philippines.

Results

Overall, bovine vaccination resulted in a statistically significant decrease …

Livestock Cattle As A Predictor Of Schistosomiasis Transmission In Nigeria, Oyetunde T. Oyeyemi, Oluyemi A. Okunlola, Zhiqiang Fu, Yang Hong, Jun-Hu Chen, Lisa M. Shollenberger

Biological Sciences Faculty Publications

Livestock cattle have been widely linked to schistosomiasis epidemiology, but little is known about its contribution to schistosomiasis transmission in Nigeria. The aim of this study is to examine the association between prevalence of schistosomiasis and livestock cattle index in Nigeria. The study's data came from three sources: the demographic and health survey (DHS), the malaria indicators survey (MIS), and the expanded special project for the eradication of neglected tropical diseases (ESPEN). Analysis of variance, correlation, and logistic regression were used to evaluate the mean difference in schistosomiasis prevalence across geopolitical zones, the relationship between schistosomiasis prevalence and livestock cattle, …

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Financial Strain Of Covid-19 And Its Impact On Willingness-To-Pay For Equine Care, Jada M. Thompson, Michelle Kibler, Jennie L.Z. Ivey

Agricultural Economics and Agribusiness Faculty Publications and Presentations

The novel COVID-19 virus caused a global pandemic disrupting lives, industries, and economies. The result was an impact on prices due to challenges with production and supply chain distribution. This study investigates the financial strain COVID-19 had on equine owners and leasers, what the market for equine care would bear if costs for care increased, and what factors contribute to their willingness to pay (WTP) for increasing cost of equine care. An online survey was distributed for 4 weeks to adult U.S. residents. Respondents reported their involvement in the equine industry, financial response to COVID-19, and responses to …

Equine Kinesiology Tape Application To Equine Hock Joints: Impact On Lameness And Movement Evaluation, Kiara Jones

Honors College Theses

Discomfort in the hocks of horses can cause lameness, resulting in poor performance. Diagnosis by veterinarians typically includes evaluating movement or use of ultrasound or radiography to evaluate tendons, ligaments, or bones. Treatment methods and times vary depending on diagnosis, but often include joint injections, medications, and stall rest. Treatments can be costly both financially and regarding time off from practice or competition. Alternative practices such as using equine kinesiology tape to support joints may improve comfort and movement for lower cost. The objective of this project was to evaluate the ease of use and effects of equine kinesiology tape …

Seroprevalence And Titer Concentration Testing For Leptospirosis In Equine, Ashley Himmelsbaugh

Honors College Theses

The most common best management practice used to combat diseases in horses is vaccination, which can decrease the incidence or severity of illness. However, the duration of immunity will vary for each vaccination and must be considered for revaccination purposes. Titer concentration, which evaluates antibodies in the blood, may be a helpful way of determining how long an animal has immunity to a disease. Leptospirosis, a zoonotic, bacterial disease, can result in uveitis, potentially leading to blindness, and abortion in mares. One serovar, Leptospirosis pomona (L. pomona), is associated with most cases of clinical disease in horses in North America. …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Gabapentin, A Human Therapeutic Medication And An Environmental Substance Transferring At Trace Levels To Horses: A Case Report., Kimberly Brewer, Jacob Machin, George Maylin, Clara Fenger, Abelardo Morales-Briceño, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Gabapentin, 1-(Aminomethyl)cyclohexaneacetic acid, MW 171.240, is a frequently prescribed high dose human medication that is also used recreationally. Gabapentin is orally absorbed; the dose can be 3,000 mg/day and it is excreted essentially unchanged in urine. Gabapentin is stable in the environment and routinely detected in urban wastewater. Gabapentin randomly transfers from humans to racing horses and is at times detected at pharmacologically ineffective / trace level concentrations in equine plasma and urine. In Ohio racing between January 2019 and July 2020,18 Gabapentin identifications, all less than 2 ng/ml in plasma, were reported. These identifications were ongoing because the horsemen …