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Veterinary Medicine Commons™

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2014

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Articles 301 - 330 of 356

Full-Text Articles in Veterinary Medicine

Sjl Mice Infected With Acanthamoeba Castellanii Develop Central Nervous System Autoimmunity Through The Generation Of Cross-Reactive T Cells For Myelin Antigens, Jay Reddy, Chandirasegaran Massilamany, Francine Marciano-Cabral, Bruno Da Rocha-Azevedo, Melissa Jamerson, Arunakumar Gangaplara, David Steffen, Rana Zabad, Zsolt Illes, Raymond Sobel Jan 2014

Sjl Mice Infected With Acanthamoeba Castellanii Develop Central Nervous System Autoimmunity Through The Generation Of Cross-Reactive T Cells For Myelin Antigens, Jay Reddy, Chandirasegaran Massilamany, Francine Marciano-Cabral, Bruno Da Rocha-Azevedo, Melissa Jamerson, Arunakumar Gangaplara, David Steffen, Rana Zabad, Zsolt Illes, Raymond Sobel

School of Veterinary and Biomedical Sciences: Faculty Publications

We recently reported that Acanthamoeba castellanii (ACA), an opportunistic pathogen of the central nervous system (CNS) possesses mimicry epitopes for proteolipid protein (PLP) 139–151 and myelin basic protein 89–101, and that the epitopes induce experimental autoimmune encephalomyelitis (EAE) in SJL mice reminiscent of the diseases induced with their corresponding cognate peptides. We now demonstrate that mice infected with ACA also show the generation of crossreactive T cells, predominantly for PLP 139–151, as evaluated by T cell proliferation and IAs/dextramer staining. We verified that PLP 139–151-sensitized lymphocytes generated in infected mice contained a high proportion of T helper 1 cytokineproducing cells, …

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Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee Jan 2014

Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The …

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Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard Jan 2014

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …

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Moraxella Spp. Isolated From Field Outbreaks Of Infectious Bovine Keratoconjunctivitis: A Retrospective Study Of Case Submissions From 2010 To 2013, John Dustin Loy, Bruce W. Brodersen Jan 2014

Moraxella Spp. Isolated From Field Outbreaks Of Infectious Bovine Keratoconjunctivitis: A Retrospective Study Of Case Submissions From 2010 To 2013, John Dustin Loy, Bruce W. Brodersen

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious bovine keratoconjunctivitis (IBK), also known as pinkeye, is the most costly eye disease of cattle. The principal etiologic agent of IBK is the Gram-negative bacterium Moraxella bovis. However, there have been reports of IBK outbreaks associated with Moraxella bovoculi. A retrospective study of IBK diagnostic cases submitted from July 1, 2010, through October 31, 2013, was conducted. Included in the study were 1,042 Moraxella isolates from 1,538 swabs of lacrimal secretions collected from 282 herds from 30 U.S. states. Moraxella isolates were identified to the species level and were composed of M. bovoculi (701 isolates), M. bovis …

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Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones Jan 2014

Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones

Nebraska Center for Virology: Faculty Publications

Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had …

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Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz Jan 2014

Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz

School of Veterinary and Biomedical Sciences: Faculty Publications

Infections with bovine viral diarrhea virus (BVDV) of the genus pestivirus, family Flaviviridae, are not limited to cattle but occur in various artiodactyls. Persistently infected (PI) cattle are the main source of BVDV. Persistent infections also occur in heterologous hosts such as sheep and deer. BVDV infections of goats commonly result in reproductive disease, but viable PI goats are rare. Using 2 BVDV isolates, previously demonstrated to cause PI cattle and white-tailed deer, this study evaluated the outcome of experimental infection of pregnant goats. Pregnant goats (5 goats/group) were intranasally inoculated with BVDV 1b AU526 (group 1) or BVDV 2 …

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Glutaredoxin-2 Is Required To Control Oxidative Phosphorylation In Cardiac Muscle By Mediating Deglutathionylation Reactions, Mary-Ellen Harper, Ryan J. Mailloux, Jian Ying Xuan, Skye Mcbride, Wael Maharsy, Stephanie Thorn, Chet E. Holterman, Christopher R.J. Kennedy, Peter Rippstein, Robert Dekemp, Jean Da Silva, Mona Nemer, Marjorie Lou Jan 2014

Glutaredoxin-2 Is Required To Control Oxidative Phosphorylation In Cardiac Muscle By Mediating Deglutathionylation Reactions, Mary-Ellen Harper, Ryan J. Mailloux, Jian Ying Xuan, Skye Mcbride, Wael Maharsy, Stephanie Thorn, Chet E. Holterman, Christopher R.J. Kennedy, Peter Rippstein, Robert Dekemp, Jean Da Silva, Mona Nemer, Marjorie Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin-2 (Grx2) modulates the activity of several mitochondrial proteins in cardiac tissue by catalyzing deglutathionylation reactions. However, it remains uncertain whether Grx2 is required to control mitochondrial ATP output in heart. Here, we report that Grx2 plays a vital role modulating mitochondrial energetics and heart physiology by mediating the deglutathionylation of mitochondrial proteins. Deletion of Grx2 (Grx2−/−) decreased ATP production by complex I-linked substrates to half that in wild type (WT) mitochondria. Decreased respiration was associated with increased complex I glutathionylation diminishing its activity. Tissue glucose uptake was concomitantly increased. Mitochondrial ATP output and complex I activity could be recovered …

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The Catabolite Control Protein E (Ccpe) Affects Virulence Determinant Production And Pathogenesis Of Staphylococcus Aureus, Torsten Hartmann, Grégory Baronian, Nadine Nippe, Meike Voss, Bettina Schulthess, Christiane Wolz, Janina Eisenbeis, Kerstin Schmidt-Hohagen, Rosmarie Gaupp, Cord Sunderkötter, Christoph Beisswenger, Robert Bals, Greg A. Somerville, Mathias Herrmann, Virginie Molle, Markus Bischoff Jan 2014

The Catabolite Control Protein E (Ccpe) Affects Virulence Determinant Production And Pathogenesis Of Staphylococcus Aureus, Torsten Hartmann, Grégory Baronian, Nadine Nippe, Meike Voss, Bettina Schulthess, Christiane Wolz, Janina Eisenbeis, Kerstin Schmidt-Hohagen, Rosmarie Gaupp, Cord Sunderkötter, Christoph Beisswenger, Robert Bals, Greg A. Somerville, Mathias Herrmann, Virginie Molle, Markus Bischoff

School of Veterinary and Biomedical Sciences: Faculty Publications

Carbon metabolism and virulence determinant production are often linked in pathogenic bacteria, and several regulatory elements have been reported to mediate this linkage in Staphylococcus aureus. Previously, we described a novel protein, catabolite control protein E (CcpE) that functions as a regulator of the tricarboxylic acid cycle. Here we demonstrate that CcpE also regulates virulence determinant biosynthesis and pathogenesis. Specifically, deletion of ccpE in S. aureus strain Newman revealed that CcpE affects transcription of virulence factors such as capA, the first gene in the capsule biosynthetic operon; hla, encoding α-toxin; and psmα, encoding the phenol-soluble modulin cluster α. Electrophoretic mobility …

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Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell Jan 2014

Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell

School of Veterinary and Biomedical Sciences: Faculty Publications

Vitamin D is an important hormone in vertebrates. Most animals acquire this hormone through their diet, secondary to exposure to ultraviolet B (UVB) radiation, or a combination thereof. The objectives for this research were to evaluate the clinical and physiologic effects of artificial UVB light supplementation on guinea pigs (Cavia porcellus) and to evaluate the long-term safety of artificial UVB light supplementation over the course of six months. Twelve juvenile acromelanic Hartley guinea pigs were randomly assigned to one of two treatment groups: Group A was exposed to 12 hours of artificial UVB radiation daily and Group B …

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Influence Of Aedes Aegypti Saliva On The Vertebrate Host Response To Dengue Virus, Michael Kevin Mccracken Jan 2014

Influence Of Aedes Aegypti Saliva On The Vertebrate Host Response To Dengue Virus, Michael Kevin Mccracken

LSU Doctoral Dissertations

Dengue virus (DENV) is maintained in a primarily anthroponotic cycle between humans and the mosquito, Aedes aegypti. Investigations into DENV infection of the vertebrate host generally do not account for the contribution of vector saliva, an inherent part of the mosquito-borne viral inoculum. Feeding by mosquitoes on vertebrate hosts is initiated by probing, which results in physical damage to the skin and vasculature, and the simultaneous introduction of DENV and saliva into the skin. Saliva contains many individual proteins with the potential to modulate host hemostasis and immune responses, thereby facilitating blood feeding and virus transmission. As exogenous antigens, both …

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Genetic Characterization Of Paramphistomes Of Buffalo By Hat-Rapd Analysis, Giregowdanahalli Puttalakshmamma, Umed Ramani, Krishna Singh, Amrutlal Patel, Abdul Patel, Chaitanya Joshi Jan 2014

Genetic Characterization Of Paramphistomes Of Buffalo By Hat-Rapd Analysis, Giregowdanahalli Puttalakshmamma, Umed Ramani, Krishna Singh, Amrutlal Patel, Abdul Patel, Chaitanya Joshi

Turkish Journal of Veterinary & Animal Sciences

Paramphistomosis is widely prevalent in domestic farm animals, incurring heavy economic losses in several countries across the world. As the identification of these parasites based on morphological features is very difficult, molecular characterization is necessary to discriminate among different species. We performed polymorphic DNA fingerprint analysis of 3 different species of amphistomes collected from the rumen and bile ducts of buffaloes slaughtered at a local abattoir in Anand, Gujarat, India. The ruminal amphistomes were identified as Paramphistomum cervi and Gastrothylax indicus, while the hepatic amphistomes were identified as Gigantocotyle bathycotyle. High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis with 4 …

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Antioxidant Effect Of Rosehip Seed Powder In Raw And Cooked Meatballs During Refrigerated Storage, Huri̇ İlyasoğlu Jan 2014

Antioxidant Effect Of Rosehip Seed Powder In Raw And Cooked Meatballs During Refrigerated Storage, Huri̇ İlyasoğlu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to learn the usage ability of rosehip seed powder (RSP) as an antioxidant agent to prevent lipid oxidation in raw and cooked meatballs during refrigerated storage. For this purpose, 4 different formulations (without RSP and with 1%, 2%, and 4% RSP) were prepared. Thiobarbituric acid reactive substances (TBARS) values of the raw and cooked meatballs were determined at 0, 3, 6, 9, and 12 days of storage. Both the raw and the cooked meatballs containing 2% RSP had lower TBARS values at the end of storage compared to the control meatballs. Moreover, the difference …

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Relationship Between Degree Of Anemia And Blood Gases In Cattle With Theileriosis, Mehmet Temi̇z, Nuri̇ Altuğ, Nazmi̇ Yüksek Jan 2014

Relationship Between Degree Of Anemia And Blood Gases In Cattle With Theileriosis, Mehmet Temi̇z, Nuri̇ Altuğ, Nazmi̇ Yüksek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the relationship between the degrees of anemia and blood gases in cattle with theileriosis. It included 28 cattle with theileriosis and 7 healthy animals as the control group. The cattle with theileriosis were divided into 4 groups according to their hematocrit (Hct) values: cattle with Hct of >26 were considered as nonanemic (Group 1), Hct of 20-26 as mildly anemic (Group 2), Hct of 14-19 as moderately anemic (Group 3), and Hct of 10-13 as severely anemic (Group 4). The PCO_2, HCO_3, and TCO_2 levels in the study groups were lower than those of …

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Ultrasonographic And Clinicopathological Findings In A 7-Year-Old Mare With Urine Retention, Hussein Hussein Jan 2014

Ultrasonographic And Clinicopathological Findings In A 7-Year-Old Mare With Urine Retention, Hussein Hussein

Turkish Journal of Veterinary & Animal Sciences

A 7-year-old crossbreed mare presented with mild to moderate colic and a 2-day history of anuria. Clinicopathological findings revealed leukocytosis with mild neutrophilia and lymphocytosis. Urinalysis showed increased leukocytes, epithelial cells, and protein. Biochemical analysis revealed mild azotemia and hypoglycemia. Ultrasonographically, the urethra was completely blocked by echoic material. The wall of the urethra was hyperechoic and easy to distinguish from the urethralis muscle surrounding it. The urinary bladder was distended, with a rough, thick wall (9 mm). To the best of the author's knowledge, the present report is the first to describe detailed ultrasonographic and clinicopathological findings in a …

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Association Of Molecular Polymorphism In Exon 2 Of Ovar-Drb1 Gene With Weight Traits In Iranian Makuie Sheep Breed, Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian Jan 2014

Association Of Molecular Polymorphism In Exon 2 Of Ovar-Drb1 Gene With Weight Traits In Iranian Makuie Sheep Breed, Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian

Turkish Journal of Veterinary & Animal Sciences

Exon 2 of the Ovar-DRB1 gene of 90 Makuie sheep was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Ten alleles and 18 genotypes were identified. The frequencies of the genotypes were 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487, and 0.0365 for AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ, and AV, respectively. Allele frequencies were 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854, and 0.0183 for A, B, E, F, I, M, O, P, …

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Disturbance Of Antioxidant Protection And Natural Resistance Factors In Rats With Different Availabilities Of Trivalent Chromium, Vasyl Vlizlo, Ruslana Iskra, Ihor Maksymovych, Marcin Wojciech Lis, Jerzy Wojciech Niedziólka Jan 2014

Disturbance Of Antioxidant Protection And Natural Resistance Factors In Rats With Different Availabilities Of Trivalent Chromium, Vasyl Vlizlo, Ruslana Iskra, Ihor Maksymovych, Marcin Wojciech Lis, Jerzy Wojciech Niedziólka

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to examine the influence of trivalent chromium ions (Cr^{3+}) supplementation on antioxidant status and immune defense of rats. During the 30 days of the experiment, the male rats (n = 10 per group) were supplemented with CrCl_3, dissolved in distilled drinking water in doses of 0, 70, or 140 µg Cr^{3+}/dm^3. Next, the contents of hydroperoxides of lipids (HPL) and thiobarbituric acid reactive substances (TBARS) in plasma were determined, as well as the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and reduced glutathione (GSH) in erythrocytes. Lysozymic and …

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Study Of Beef Blade Muscles' Differentiation Depending On Conformation And Fat Class, Dominika Guzek, Dominika Glabska, Ewa Lange, Krzysztof Glabski, Agnieszka Wierzbicka Jan 2014

Study Of Beef Blade Muscles' Differentiation Depending On Conformation And Fat Class, Dominika Guzek, Dominika Glabska, Ewa Lange, Krzysztof Glabski, Agnieszka Wierzbicka

Turkish Journal of Veterinary & Animal Sciences

The object of this study was to identify variation of the intramuscular fat and connective tissue content in different blade muscles in carcasses characterized by various quality grades. It was found that there is a cumulative impact of muscle and conformation class on intramuscular fat in blade muscle (P = 0.0330), as well as type of muscle and fat class (P = 0.0424), but there is no cumulative impact of conformation class and fat class (P = 0.1788). There is no cumulative influence of muscle type, conformation class, and fat class on amount of connective tissue in blade muscle, but …

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Ameliorative Efficacy Of Citrus Fruit Oil In Aflatoxicosis In Broilers: A Growth And Biochemical Study, Senthil Kumar Dhanapal, Suguna Rao, Pradeep Kumar Palahally Govindaraju, Ravikumar Hukkeri, Karikalan Mathesh Jan 2014

Ameliorative Efficacy Of Citrus Fruit Oil In Aflatoxicosis In Broilers: A Growth And Biochemical Study, Senthil Kumar Dhanapal, Suguna Rao, Pradeep Kumar Palahally Govindaraju, Ravikumar Hukkeri, Karikalan Mathesh

Turkish Journal of Veterinary & Animal Sciences

An experiment was conducted to study the efficacy of citrus fruit oil on growth parameters and biochemical parameters of broilers in 4 groups with 2 replicates of 20 chicks in each. Standard broiler diet without aflatoxin was given to group A, basal diet + citrus fruit oil 2.5 g kg^{-1} to group B, basal diet + 1 ppm aflatoxin to group C, and 1 ppm aflatoxin + basal diet + citrus fruit oil 2.5 g kg^{-1} to group D. The results showed that supplementation in group D had a moderate effect on posttreatment growth performance, relative organ weight, and biochemical …

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Treatment Of Canine Transmissible Venereal Tumor Using L-Asparaginase, Prednisone, And Surgery In A Clinical Chemotherapy-Resistant Case, Daniella Matos Da Silva, Mhayara Samile De Oliveira Reusing, Aline Iara Franciosi, Carlos Edvardo Penner Belo, Kamilá Alcala Gonçalves, Renato Silva De Sousa, Simone Domit Guérios Jan 2014

Treatment Of Canine Transmissible Venereal Tumor Using L-Asparaginase, Prednisone, And Surgery In A Clinical Chemotherapy-Resistant Case, Daniella Matos Da Silva, Mhayara Samile De Oliveira Reusing, Aline Iara Franciosi, Carlos Edvardo Penner Belo, Kamilá Alcala Gonçalves, Renato Silva De Sousa, Simone Domit Guérios

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old intact female dog was presented with a vaginal transmissible venereal tumor (TVT) that had clinically developed multidrug resistance to vincristine, vinblastine, and doxorubicin. Complete remission was obtained with combined-modality therapy with surgery, L-asparaginase, and prednisone.

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Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü Jan 2014

Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü

Turkish Journal of Veterinary & Animal Sciences

Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, …

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Course Of Hatch And Developmental Changes In Thyroid Hormone Concentration In Blood Of Chicken Embryo Following In Ovo Riboflavin Supplementation, Karolina Barbara Trzeciak, Marcin Wojciech Lis, Andrzej Sechman, Barbara Plytycz, Agata Rudolf, Tomasz Wojnar, Jerzy Wojcciech Niedziólka Jan 2014

Course Of Hatch And Developmental Changes In Thyroid Hormone Concentration In Blood Of Chicken Embryo Following In Ovo Riboflavin Supplementation, Karolina Barbara Trzeciak, Marcin Wojciech Lis, Andrzej Sechman, Barbara Plytycz, Agata Rudolf, Tomasz Wojnar, Jerzy Wojcciech Niedziólka

Turkish Journal of Veterinary & Animal Sciences

The influence of riboflavin on the function of the hypothalamo-pituitary-thyroid axis during chicken embryogenesis is poorly understood. Therefore, examination of the effects of in ovo riboflavin supplementation on the possible linkage between the thyroid gland function and chick embryonic development seems to be interesting. Eggs on the sixth day of incubation were injected with 0 (control), 60, or 600 µg of riboflavin/egg. Blood samples were collected on the 12th, 15th, 18th, and 20th days of embryogenesis. Thyroxine (T_4) and triiodothyronine (T_3) concentrations in plasma samples were determined by radioimmunoassay method. The time of external pipping and hatching of each chick …

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In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz Jan 2014

In Vivo Embryo Production In Some Native Goat Breeds In Turkey, Ali̇ Reha Ağaoğlu, Kübra Karakaş, Mustafa Kaymaz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the efficacy of the repeated administration of superovulatory protocol and surgical procedure in in vivo embryo production, as well as to determine the differences in the responses to superovulatory treatment among several Turkish native goat breeds (Angora, Kilis, Honamli, Hair, and Norduz). A superovulatory treatment procedure was repeated 3 times in this study. The estrus cycles were synchronized and superovulatory response was induced with porcine follicle-stimulating hormone during the natural breeding season. Natural mating was performed following CIDR removal. Embryo recovery was performed surgically on day 6, after the first mating. According …

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Inhibitory Effect Of K^+ And Ca^{2+} Concentrations, Ph, And Osmolality Of Activation Solution On Motility Of Shabut (Barbus Grypus Heckel 1843) Spermatozoa, Fati̇h Öğretmen, Selami̇ Gölbaşi, Burak Evren İnanan Jan 2014

Inhibitory Effect Of K^+ And Ca^{2+} Concentrations, Ph, And Osmolality Of Activation Solution On Motility Of Shabut (Barbus Grypus Heckel 1843) Spermatozoa, Fati̇h Öğretmen, Selami̇ Gölbaşi, Burak Evren İnanan

Turkish Journal of Veterinary & Animal Sciences

Evaluation of sperm quality is important in order to understand the main biochemical processes occurring during motility of sperm, to determine the reproductive ability of fish species, and to optimize the environment for spermatozoa. Generally, once differentiated in the gonad the sperm remain there completely quiescent until they are released into an external medium. After activation, motility duration of fish spermatozoa is greatly affected by environmental factors such as pH, ion concentrations, and osmotic pressure. Investigating these factors in relation to fish sperm can help establish good activation or immobilizing media for improving artificial fertilization and preservation. This study aimed …

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In Vitro Assessment On Intestinal Microflora From Commonly Farmed Fishes For Control Of The Fish Pathogen Edwardsiella Tarda, Jiun Yan Loh, Yau Yan Lim, Sharr Azni Harmin, Adeline Su Yien Ting Jan 2014

In Vitro Assessment On Intestinal Microflora From Commonly Farmed Fishes For Control Of The Fish Pathogen Edwardsiella Tarda, Jiun Yan Loh, Yau Yan Lim, Sharr Azni Harmin, Adeline Su Yien Ting

Turkish Journal of Veterinary & Animal Sciences

This study was aimed to evaluate the microflora isolated from the intestines of tilapia (Oreochromis niloticus), freshwater catfish (Clarias batrachus), snakehead (Ophicephalus striatus), snakeskin gourami (Trichogaster pectoralis), climbing perch (Anabas testudineus), and river catfish (Pangasius pangasius) for their potential probiotic development. Isolates were initially screened for their inhibitory effect against one of the most common fish pathogens, Edwardsiella tarda. Positive antagonists were subsequently tested for hemolytic activity, antibiotic susceptibility, and production of bacteriocin-like inhibitory substances (BLISs). Our results revealed 55 of the 405 isolates tested showed inhibitory effects towards the pathogen. Forty isolates were γ-hemolytic bacteria. Only 7 isolates were …

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The Effects Of Dietary Glutamine Supplementation On Growth Performance And Intestinal Morphology Of Broiler Chickens Reared Under Hot Conditions, Farhad Jazideh, Parviz Farhoomand, Mohsen Daneshyar, Gholamreza Najafi Jan 2014

The Effects Of Dietary Glutamine Supplementation On Growth Performance And Intestinal Morphology Of Broiler Chickens Reared Under Hot Conditions, Farhad Jazideh, Parviz Farhoomand, Mohsen Daneshyar, Gholamreza Najafi

Turkish Journal of Veterinary & Animal Sciences

Dietary supplementation effects of 0%, 0.25%, 0.5%, and 1% glutamine were investigated on the performance and development of the gastrointestinal tract in broilers reared under hot conditions. No significant differences were observed between the treatments for feed intake and feed conversion ratio during the starter period, grower period, or entire experiment (P > 0.05). During the grower period, only 0.5% glutamine-fed birds had higher body weight gain than others (P < 0.05). For the entire period, body weight gain of 0.5% glutamine-fed birds was greater than that of 0.00% and 0.25% glutamine-fed ones (P < 0.05). Furthermore, 0.5% glutamine-fed birds showed longer villus height than other birds (P < 0.05). In the jejunum, both 0.5% and 1% glutamine consumption caused longer villus height as compared to 0.00% and 0.25% glutamine (P < 0.05). Villus height of 0.25% glutamine-fed birds was higher than that of 0.00% glutamine-fed ones (P < 0.05). None of the ileum morphological parameters were affected by glutamine supplementation (P > 0.05). In conclusion, supplementation of 0.5% glutamine improved the intestinal morphology and body weight gain of broilers under heat stress. Higher dietary glutamine (1%) increased the villus height in the jejunum …

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The Effects Of Royal Jelly On Performance And Fatty Acid Profiles Of Different Tissues In Quail (Coturnix Coturnix Japonica) Reared Under High Stocking Density, İsmai̇l Seven, Ülkü Gülci̇han Şi̇mşek, Zehra Gökçe, Pinar Tatli Seven, Aslihan Arslan, Ökkeş Yilmaz Jan 2014

The Effects Of Royal Jelly On Performance And Fatty Acid Profiles Of Different Tissues In Quail (Coturnix Coturnix Japonica) Reared Under High Stocking Density, İsmai̇l Seven, Ülkü Gülci̇han Şi̇mşek, Zehra Gökçe, Pinar Tatli Seven, Aslihan Arslan, Ökkeş Yilmaz

Turkish Journal of Veterinary & Animal Sciences

To study the effects of royal jelly (RJ) on performance and fatty acid profiles, 168 unsexed 8-day-old quails (Coturnix coturnix japonica) were assigned to 4 treatment groups. Groups were arranged as follows: control (LSD; 160 cm2/quail and no supplementation), high stocking density (HSD; 80 cm2/quail and no supplementation), HSD-RJ 250 [80 cm2/quail and 250 mg/kg body weight (BW) RJ given orally], and HSD-RJ 500 (80 cm2/quail and 500 mg/kg BW RJ given orally). Body weight gain decreased significantly in the HSD group at day 42 (P < 0.01). Feed intake (FI) and feed conversion rate (FCR) were also significantly affected in the HSD group (P < 0.01). FI and FCR were improved by both doses of RJ. Both RJ doses increased total polyunsaturated fatty acid ratios in the breast, kidney (P < 0.001), leg, and liver (P < 0.05). Total saturated fatty acid ratios in leg (P < 0.01) and kidney (P < 0.05) tissues increased in the HSD group but decreased in the livers of HSD-RJ 250 group (P < 0.05). In conclusion, RJ supplementation improved performance parameters and unsaturated fatty acid ratios in the examined quail tissues.

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Serological Survey Of Equid Herpesvirus 3 Infection In Turkish Horses, Veysel Soydal Ataseven, Firat Doğan, Seval Bi̇lge Dağalp, Mehmet Tolga Tan, Köksal Altinbay, Cafer Tayyar Ateş Jan 2014

Serological Survey Of Equid Herpesvirus 3 Infection In Turkish Horses, Veysel Soydal Ataseven, Firat Doğan, Seval Bi̇lge Dağalp, Mehmet Tolga Tan, Köksal Altinbay, Cafer Tayyar Ateş

Turkish Journal of Veterinary & Animal Sciences

Sera from 1133 Turkish horses were obtained and tested for equid herpesvirus 3 (EHV-3) antibodies using a sero-neutralisation test. The sample consisted of 420 privately owned working horses from 2 regions of Turkey, 428 brood horses from private stud farms and breeding stations, and 285 racehorses from private stud farms. Neutralising antibodies against EHV-3 were found in 287 (25.3%) of the 1133 analysed sera. The highest seroprevalence for EHV-3 was detected in the brood horses (51.2%), followed by the racehorses (10.2%), and the working horses (9.3%). The seroprevalence rates tended to be higher in female horses than in male horses …

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Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük Jan 2014

Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük

Turkish Journal of Veterinary & Animal Sciences

The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the …

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Milk Fatty Acid Composition From Goats In A Semiintensive Production System In An Arid Region Of The Peninsula Of Baja California, Mexico, Eduardo Alberto Toyes Vargas, Hector Gonzalez Garcia, Miguel Victor Cordoba Matson, Ricardo Ortega Perez, Jose Luis Espinoza Villavicencio, Alejandro Palacios Espinosa, Narciso Ysac Avila Serrano, Bernardo Murillo Amador Jan 2014

Milk Fatty Acid Composition From Goats In A Semiintensive Production System In An Arid Region Of The Peninsula Of Baja California, Mexico, Eduardo Alberto Toyes Vargas, Hector Gonzalez Garcia, Miguel Victor Cordoba Matson, Ricardo Ortega Perez, Jose Luis Espinoza Villavicencio, Alejandro Palacios Espinosa, Narciso Ysac Avila Serrano, Bernardo Murillo Amador

Turkish Journal of Veterinary & Animal Sciences

The aim of this research was to study the effects of seasons (rainy and dry) on the composition of the fatty acids (FAs) in milk of Creole goats in a semiintensive production system. Milk sampling was carried out in the dry season (June) and just after the rainy season (November) in an arid region of Baja California Sur, Mexico. The goat diet was composed of native species it grazed on in the rangeland, as well as hayed forages with some supplements containing approximately equal amounts of sorghum and maize. The results for different types of FAs in goat milk showed …

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Nmr-Based Metabolomic Evaluation In Dairy Cows With Displaced Abomasum, Abdullah Başoğlu, Nuri̇ Başpinar, Alparslan Coşkun Jan 2014

Nmr-Based Metabolomic Evaluation In Dairy Cows With Displaced Abomasum, Abdullah Başoğlu, Nuri̇ Başpinar, Alparslan Coşkun

Turkish Journal of Veterinary & Animal Sciences

This study is the first to evaluate nuclear magnetic resonance (NMR)-based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were …

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