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Articles 1 - 4 of 4
Full-Text Articles in Veterinary Medicine
Monitoring Ovarian Cycles By Fecal Progesterone Analysis In Thamin Eld’S Deer Hinds (Rucervus Eldii Thamin), Orasa Phraluk, Chainarong Punkong, Ampika Thongphakdee, Boripat Siriaroonrat, Worawidh Wajjwalku, Nikorn Thongtip
Monitoring Ovarian Cycles By Fecal Progesterone Analysis In Thamin Eld’S Deer Hinds (Rucervus Eldii Thamin), Orasa Phraluk, Chainarong Punkong, Ampika Thongphakdee, Boripat Siriaroonrat, Worawidh Wajjwalku, Nikorn Thongtip
The Thai Journal of Veterinary Medicine
The aims of this study were to demonstrate the effectiveness of fecal progesterone metabolite monitoring technique during estrus synchronization and to investigate fecal progesterone metabolite profile in Eld’s deer (Rucervus eldii thamin) hinds for one year (February 2009-Janurary 2010). Fecal samples from five hinds were collected weekly, subjected to hormone extraction and subsequently progesterone analysis using enzyme immunoassay (EIA). Estrus synchronization and artificial insemination were performed during breeding season (June 2009). This included an intramuscular injection of ProstaglandinF2α (5 mg) in combination with a vaginal insertion of a controlled internal drug-releasing device (CIDR type G) for 14 d. After CIDR-G …
Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman
Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman
Animal Science
No abstract provided.
Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm
Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm
Turkish Journal of Veterinary & Animal Sciences
Three-\beta-hydroxysteroid dehydrogenase (3\beta-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3\beta-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + …
The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek
The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the complementary effects of luteinizing hormone (LH) on cell subpopulations. Corpora lutea were collected on days 5 and 15 of the cycle. The luteal tissues were treated with collagenase for cell dissociation. Percoll density-gradient centrifugation was performed to separate cells into 2 distinct subpopulations. Luteal cells (5 × 104 cells/well) were cultured with and without 22(R)-hydroxycholesterol (22R-HC, 10 µg/mL) and LH (10 and 100 ng/mL) alone, and also with 22R-HC (10 µg/mL) plus LH (10 and 100 ng/mL), in a serum-free culture medium for 6 days of incubation. Untreated cells isolated from …