Veterinary Medicine Commons^™

Review Of The Pathogenesis And Treatment Of Acute Spinal Cord Injury And Investigation Into The Use Of Urine 3-Hpma As A Novel Biomarker Of Secondary Injury After Acute Spinal Cord Injury In The Dog, Andrea Sangster

Open Access Theses

Acute spinal cord injury (ASCI) has two pathophysiological stages of injury: the primary injury and the secondary injury cascade. Primary injury includes the initial or mechanical insult to the spinal cord. Secondary injury is a cascade of biochemical events that propagates damage of adjacent, healthy tissue increasing the overall volume of spinal cord tissue that is affected. Acrolein is a toxic byproduct of lipid peroxidation produced during secondary injury. A metabolite of acrolein-glutathione adduct found in urine, 3-HPMA, has recently been shown to increase after spinal cord injury in a rat model. The aim of our study was to apply …

The Effects Of Heat Stress On Immunity In Laying Hens And Dairy Cattle, Rebecca Ann Strong

Open Access Theses

With the increase in global climate change and the population growth driving the high demand for additional food production, heat stress (HS) is a major concern in the livestock industry across all species. Animals experience HS when exposed to high environmental temperatures outside their thermal neutral zone. The level of the effects can vary due to the length and intensity of HS to which the animal is exposed to. In experiment one, laying hens with access to cooled perches during HS had a lower heterophil to lymphocyte ratio compared to the control hens after 4 h of acute heat stress, …

Cut-Off Values For Gait Variables To Detect Forelimb Lameness In Individual Dogs, Jennifer G. Carr

Open Access Theses

The objective of this study was to characterize kinetic and kinematic variables in dogs with forelimb lameness and determine lameness cutoff values of gait variables using ROC analysis with observational gait analysis (OGA) as reference. Twenty client-owned dogs with unilateral lameness were included. Dogs underwent orthopedic exam, including OGA, and instrumented gait analysis (IGA; kinetic and kinematic analysis). Kinetic variables with the highest accuracy were PVF and %WD with an area under the curve (AUC) of 0.73 and 0.92, respectively. Optimal cutoff value for PVF and %WD were ≤ 10.6 (sensitivity 70% and specificity 75%) and ≤ 29.7 (sensitivity 90% …

Pharmacokinetics And Toxicity Of The Novel Oral Demethylating Agent Zebularine In Laboratory And Tumor Bearing Dogs, Christopher Michael Fulkerson

Open Access Theses

The aim of this thesis is to investigate the pharmacokinetics and toxicity of the novel oral demethylating agent zebularine (zeb) in laboratory dogs and tumor bearing dogs. This thesis focuses on the application of this therapeutic strategy in dogs with naturally occurring invasive transitional cell carcinoma (InvTCC), which serve as a relevant model of the human disease.

DNA hypermethylation in the promoter region is a common epigenetic change in cancer that silences tumor suppressor genes. Zeb is an oral cytidine analog that acts as a demethylating agent. Zeb has been investigated extensively in vitro as well as in mice, rodents …