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Veterinary Medicine Commons™

Open Access. Powered by Scholars. Published by Universities.®

2014

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Articles 241 - 270 of 356

Full-Text Articles in Veterinary Medicine

Ractopamine Effect On Lipid Metabolism And Glut4 Amount Of Finishing Pigs, Thiago Santos Araujo, Laura Cristina Jardim Porto, Erica Guilhen Mario, Luciano José Pereira, Matheus Soares Da Silva Ferreira, Marcio Gilberto Zangeronimo, Marcelo Henrique Napimoga, Leida Maria Botion, Raimundo Vicente De Sousa Jan 2014

Ractopamine Effect On Lipid Metabolism And Glut4 Amount Of Finishing Pigs, Thiago Santos Araujo, Laura Cristina Jardim Porto, Erica Guilhen Mario, Luciano José Pereira, Matheus Soares Da Silva Ferreira, Marcio Gilberto Zangeronimo, Marcelo Henrique Napimoga, Leida Maria Botion, Raimundo Vicente De Sousa

Turkish Journal of Veterinary & Animal Sciences

Ractopamine (RAC) causes fat deposition and/or fatty acid synthesis reduction and increases the rate of protein synthesis and muscle growth. However, there are few scientific studies detailing the mechanism of action of RAC and its possible metabolic pathways in swine. The objective of this study was to evaluate the effect of RAC on lipid metabolism of finishing pigs. Subcutaneous and retroperitoneal fat, muscle, and blood samples were collected at slaughter. Forty pigs were fed different RAC levels (ppm): 0, 5, 10, 15, and 20. RAC did not affect lipoprotein lipase activity in any of the tissues. There were no changes …

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Effects Of Spirulina In Arsenic Poisoning In The Black Bengal Goat, Amalendu Ghosh, Abdul Awal, Abu Hadi Noor Ali Khan, Golam Shahi Alam, Shafiqul Islam, Abu Saleh Mahfuzul Bari Jan 2014

Effects Of Spirulina In Arsenic Poisoning In The Black Bengal Goat, Amalendu Ghosh, Abdul Awal, Abu Hadi Noor Ali Khan, Golam Shahi Alam, Shafiqul Islam, Abu Saleh Mahfuzul Bari

Turkish Journal of Veterinary & Animal Sciences

Some of the pharmacological properties of spirulina (Spirulina platensis) may be linked to its antioxidant potential, which mitigates oxidative stresses. In this study we examined whether spirulina mitigated arsenic-induced toxicity in the Black Bengal goat (Capra hircus). Nonpregnant female goats of approximately 12 months old were used in noninduced, nontreated control (T_1); arsenic-induced, nontreated (T_2); and arsenic-induced, spirulina-treated (T_3) groups. Groups T_2 and T_3 were given sodium arsenite orally (5 mg/kg) daily for 15 weeks. For therapeutic justification of spirulina, 1 group of arsenic-treated goats (T_3) was given spirulina at 0, 50, 100, and 150 mg/kg daily for another 15 …

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Comparison Of Manual Measurements And Computer-Assisted Image Analysis In Fish Morphometry, Miloslav Petrtyl, Lukás Kalous, Devri̇m Memi̇ş Jan 2014

Comparison Of Manual Measurements And Computer-Assisted Image Analysis In Fish Morphometry, Miloslav Petrtyl, Lukás Kalous, Devri̇m Memi̇ş

Turkish Journal of Veterinary & Animal Sciences

Fish usually have a simple body shape and many morphological characters can be easily obtained by 2-dimensional image analysis. Measuring errors are often analyzed using data from several researchers or repeated measurements, but the testing of accuracy and repeatability based on different methods has rarely been examined. Here we report the comparison of manual measurements using Proma 150D digital calipers and computer-assisted image analysis in LUCIA software to evaluate the accuracy of both methods and the suitability of digital images for exploration of fish morphology. Eighteen morphometric characters of 20 specimens of Prussian carp were examined by 2 researchers. The …

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The Lumbar Dorsal Rami Of The Wild Pig: The Intermediate Branch, İbrahi̇m Kürtül, Sevi̇nç Ateş, Erdal Özmen, Lutfi̇ Takci, Hanno Steinke Jan 2014

The Lumbar Dorsal Rami Of The Wild Pig: The Intermediate Branch, İbrahi̇m Kürtül, Sevi̇nç Ateş, Erdal Özmen, Lutfi̇ Takci, Hanno Steinke

Turkish Journal of Veterinary & Animal Sciences

This study has documented the intermediate branch of the dorsal rami of the spinal nerves in the lumbar region of 4 adult wild pigs. The lumbar spinal nerves at the L1-L4 levels left the vertebral canal through the intervertebral foramen. Upon the dissection of the 32 dorsal rami at these levels, 24 were displayed to construct 3 discrete branches while the remaining 8 were observed to form 2 typical branches. The dorsal rami were remarkably short, giving their branches in a fan-like spreading fashion. The lateral branches sent 2 to 3 smaller subbranches to the iliocostalis lumborum muscle, then became …

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Management Of Severe Penile Swelling Associated With Urethropexy In An English Bulldog, Stefania Rabidi, Lysimachos Papazoglou, Paraskevi Tsobanidou, Wala Haddadin Jan 2014

Management Of Severe Penile Swelling Associated With Urethropexy In An English Bulldog, Stefania Rabidi, Lysimachos Papazoglou, Paraskevi Tsobanidou, Wala Haddadin

Turkish Journal of Veterinary & Animal Sciences

A 10-month-old, unilateral cryptorchid, male English bulldog underwent urethropexy for the treatment of urethral prolapse. Six hours after surgery the dog developed dysuria, haematuria, discomfort, severe penile oedema, inflammation, and inability to extrude the penis from the prepuce. Urethral patency was restored by placing an indwelling catheter into the urethral lumen. Penile swelling was managed with cold and warm compresses for 2 days, and antibiotics, nonsteroidal anti-inflammatory drugs, and opioids for 4 days. Complete resolution of the swelling was evident by 3 days after surgery. One year after surgery the dog was reported to be in good health.

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Caspase-1, -2, And -3 Gene Expression Is Enhanced In The Heart And Lung Of Chickens With Pulmonary Hypertension (Ascites), Hossein Hassanpour, Masood Teshfam, Hassan Momtaz, Hamed Zarei, Shahab Bahadoran Jan 2014

Caspase-1, -2, And -3 Gene Expression Is Enhanced In The Heart And Lung Of Chickens With Pulmonary Hypertension (Ascites), Hossein Hassanpour, Masood Teshfam, Hassan Momtaz, Hamed Zarei, Shahab Bahadoran

Turkish Journal of Veterinary & Animal Sciences

To investigate the involvement of apoptosis in the pathogenesis of T3-induced pulmonary hypertension in broiler chickens, caspase-1, -2, and -3 gene expressions were evaluated by semiquantitative RT-PCR in the lungs and left and right heart ventricles. One-day-old fast-growing chickens were randomly divided into 2 equal groups with 3 replicates per group. Chicks were reared for 7 weeks under standard conditions. T3 as a thyroid hormone was added to the ration after week 1 of rearing. Pulmonary hypertension was induced at 49 days based on RV/TV ratio index. The caspase genes were constitutively expressed in the lung and right and left …

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Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez Jan 2014

Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study is to investigate the effect of exogenous administration of melatonin, which is a strong antioxidant agent, on the gene expression of glutathione peroxidase, which is one of the antioxidant enzymes, in the livers of mice and also to examine the immunohistochemical localization of the enzyme. The 24 male Swiss albino mice used in the study were divided into 3 groups: experimental (n = 8), sham (n = 8), and control (n = 8). A 10 mg/kg dose of melatonin was administered intraperitoneally to the experimental group for 4 weeks. Only ethanol and a serum physiological …

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Risk Factors For Postpartum Anestrus In Crossbred Cows In Bangladesh, Md Mostofa Kamal, Md Musharraf Uddin Bhuiyan, Nasreen Parveen, Harry W. Momont, Mohammed Shamsuddin Jan 2014

Risk Factors For Postpartum Anestrus In Crossbred Cows In Bangladesh, Md Mostofa Kamal, Md Musharraf Uddin Bhuiyan, Nasreen Parveen, Harry W. Momont, Mohammed Shamsuddin

Turkish Journal of Veterinary & Animal Sciences

Ultrasonography and a structured questionnaire were used in a cross-sectional study to gather data on the prevalence and risk factors for anestrus in crossbred cows at =>60 days postpartum in 273 smallholder farms. The prevalence of anestrus was 18%. The odds ratio (OR) for true anestrus was 17.52 and 2.81 times higher (P < 0.05) in cows with poor (=3.5) body condition score (BCS), respectively, compared to those with optimal BCS (2.5-3.5), 2.82 times higher in suckled than in nonsuckled cows (P = 0.03), and 2.53 times higher in cows that calved during the cold season than in those that calved during the hot season (P = 0.03). The OR for anestrus was 1.62 times higher (P = 0.017) in cows managed by an employee than in those managed by the farmers themselves (P = 0.001), and 2.66 times higher (P = 0.003) in small farms (=11 cows). The OR was 0.71 to 0.46 times lower in farms having a guaranteed market to sell milk than those with an uncertain traditional milk market (P < 0.05). Maintaining optimal BCS of cows, farmers' training on management of cattle reproduction, and development of a market linkage to sell milk would improve the number of cows for breeding by 60 days postpartum.

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The Results Of Consecutive Superovulations In Cows By Induction With Various Exogenous Progesterone Routes, Numan Akyol, Sedat Hamdi̇ Kizil, Muharrem Satilmiş, Tahi̇r Karaşahi̇n, Serkan Erat Jan 2014

The Results Of Consecutive Superovulations In Cows By Induction With Various Exogenous Progesterone Routes, Numan Akyol, Sedat Hamdi̇ Kizil, Muharrem Satilmiş, Tahi̇r Karaşahi̇n, Serkan Erat

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the possibility of yielding more transferable embryos from cows superovulated with/without exogenous progesterone (ear implant and intravaginal device). Two experiments were conducted in Holstein Friesian cows to evaluate the effect of exogenous progesterone on the yield of corpus luteum (CL), total embryo and ova, and transferable embryos before superovulation. A superovulation program using 2 different progesterone resources was applied (G1 = intravaginal device, G2 = ear implant). Superovulation and uterus irrigations were done at 28-day intervals in the treatment groups. Cows in the control group were superovulated at 60-day intervals without application …

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A New Approach To Neospora Caninum Infection Epidemiology: Neosporosis In Integrated And Rural Dairy Farms In Turkey, Naci̇ Öcal, Hasan Tarik Atmaca, Meti̇n Koray Albay, Ahmet Deni̇z, Hakan Kalender, Kader Yildiz, Oğuz Kul Jan 2014

A New Approach To Neospora Caninum Infection Epidemiology: Neosporosis In Integrated And Rural Dairy Farms In Turkey, Naci̇ Öcal, Hasan Tarik Atmaca, Meti̇n Koray Albay, Ahmet Deni̇z, Hakan Kalender, Kader Yildiz, Oğuz Kul

Turkish Journal of Veterinary & Animal Sciences

Both cattle and dogs were examined in modern and rural dairy farms that had a history of abortion over 5%. The blood samples were collected from 427 aborted cattle and the sera were tested using a commercial ELISA test kit. Additionally, a necropsy procedure was carried out on the fetuses and calves dead within 2 months after birth; the tissue samples were evaluated by histopathologic, immunoperoxidase, and PCR techniques. Eighteen dogs in close contact with the cattle in the same field were included in the study and blood and feces samples were collected. The feces samples were analyzed by copro-PCR …

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Effect Of Live Weight And Age Of Akkaraman Ewes At Mating On Multiple Birth Rate, Growth Traits, And Survival Rate Of Lambs, Ahmet Hamdi̇ Aktaş, Şükrü Doğan Jan 2014

Effect Of Live Weight And Age Of Akkaraman Ewes At Mating On Multiple Birth Rate, Growth Traits, And Survival Rate Of Lambs, Ahmet Hamdi̇ Aktaş, Şükrü Doğan

Turkish Journal of Veterinary & Animal Sciences

This study was performed in order to determine the effect of live weight (LW) and age of Akkaraman ('White Karaman', WK) ewes at mating on the multiple birth rate (MBR), growth traits, and survival rate (SR) of lambs in breeder flocks. In the trial, data of 4452 head of ewes and 5324 head of lambs obtained from 16 WK breeder flocks in Konya Province were used. One week before the mating season ewes were weighed and separated into 5 groups according to their LW. In the research, the differences of MBR, the SR of lambs until days 60 and 120, …

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Influence Of Crossing Polish And Foreign Pig Breeds On Physicochemical Traits Of Longissimus Lumborum Muscle, Pawel Gajewczyk, Bartosz Gajewczyk, Jerzy Akincza, Tadeusz Szmanko Jan 2014

Influence Of Crossing Polish And Foreign Pig Breeds On Physicochemical Traits Of Longissimus Lumborum Muscle, Pawel Gajewczyk, Bartosz Gajewczyk, Jerzy Akincza, Tadeusz Szmanko

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was an assessment of physicochemical traits characterizing the longissimus lumborum muscle in fatteners originating from an application of various variants of commodity crossbreeding with a contribution of 2 Polish and 2 foreign breeds. The research material was collected from the right half-carcass from 180 crossbred fatteners selected randomly from a technological group (60 heads in each), with a division of gilts and hogs. The meat was subjected to laboratory examinations according to the procedures and requirements of the Association of Official Agricultural Chemists International. Pig breeds used in the crossbreeding affected the values of the …

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The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki Jan 2014

The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the effect of supplementing diets fed to ewes (in late pregnancy and during lactation or only during lactation) with Saccharomyces cerevisiae dried brewer’s yeast on the yield and chemical composition of milk and on selected parameters of humoral and cellular immunity. The investigated indicators were analyzed on days 28 and 70 of lactation. The addition of dried yeast contributed to a significant increase in milk yield, and the obtained milk was characterized by a lower somatic cell count, suggesting improved mammary gland health. Diet supplementation had a beneficial influence on the health …

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Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan Jan 2014

Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed …

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The Effect Of Supplemental Concentrate Feed On Live Weight Gain Of Yearling Heifers Over Grazing Season In Subirrigated Rangelands Of East Anatolia, Ali̇ Koç, Adem Kaya, Mehmet Keri̇m Güllap, Hali̇l İbrahi̇m Erkovan, Muhli̇s Maci̇t, Mevlüt Karaoğlu Jan 2014

The Effect Of Supplemental Concentrate Feed On Live Weight Gain Of Yearling Heifers Over Grazing Season In Subirrigated Rangelands Of East Anatolia, Ali̇ Koç, Adem Kaya, Mehmet Keri̇m Güllap, Hali̇l İbrahi̇m Erkovan, Muhli̇s Maci̇t, Mevlüt Karaoğlu

Turkish Journal of Veterinary & Animal Sciences

Animal performance declines considerably after summer dormancy during the grazing season in the steppe rangelands of East Anatolia. The aim of this study was to determine the growth performance of Holstein heifers grazed on rangeland and supported with concentrate. Forage amount, crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents of forage were sampled biweekly during the grazing season. In the experiment, heifers (250 ± 10 kg) were allocated to 1 of 3 feeding trials: 1) control, 2) grazing plus concentrate supplement fed at 1% of bodyweight once daily, and 3)grazing plus concentrate supplement fed at …

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Metazoan Parasites Of Brown Meagre (Sciaena Umbra L. 1758) Caught Near Gökçeada, Turkey, Ahmet Akmirza Jan 2014

Metazoan Parasites Of Brown Meagre (Sciaena Umbra L. 1758) Caught Near Gökçeada, Turkey, Ahmet Akmirza

Turkish Journal of Veterinary & Animal Sciences

This study was conducted between July 2011 and January 2012 in Gökçeada, Turkey, to determine the metazoan parasite fauna of brown meagre (Sciaena umbra). The metazoan parasite fauna of 53 brown meagre samples was investigated and a total of 7 parasite species [Anoiktostoma coronatum, Paracryptogonimus aloysiae (Digenea), Longicollum pagrosomi (Acanthocephala), Contracaecum fabri (Nematoda), Trachelobdella lubrica (Hirudinea), Nerocila bivittata, and praniza larvae (Isopoda)] were identified. It was determined that Paracryptogonimus aloysiae was the dominant parasite species of brown meagre with the prevalence of 66.04% in this region. Anoiktostoma coronatum and Paracryptogonimus aloysiae were reported for the first time in Turkey.

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Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni Jan 2014

Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni

Turkish Journal of Veterinary & Animal Sciences

To evaluate growth and enterotoxin production of Staphylococcus aureus in raw milk caciotta, an Italian soft cheese, experimental cheesemaking trials were carried out with and without a commercial starter. Two different inocula of selected enterotoxin-producing S. aureus strains were tested: high (mean value 5.03 log CFU/mL of milk) and low (mean value 3.22 log CFU/mL of milk). Uninoculated cheeses with milk containing 2.15 log CFU/mL mean value of coagulase-positive staphylococci were also examined. With higher inocul a coagulase-positive staphylococci counts reached 7.57 log CFU/g without starter and had significantly higher values after the acidification phase than those with starter (below …

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Autologous Adipose Derived Adult Multipotent Stromal Cells Alter The Porcine Systemic Immune And Bone Biomarkers Response To Cancellous Bone Xenografts, Jonathan Francis Bova Jan 2014

Autologous Adipose Derived Adult Multipotent Stromal Cells Alter The Porcine Systemic Immune And Bone Biomarkers Response To Cancellous Bone Xenografts, Jonathan Francis Bova

LSU Master's Theses

The use of a porcine model in assessing bone grafts in vivo is common when a large animal model is necessary. In this thesis we aimed to improve the porcine model of facial reconstruction through the use of a local anesthesia and novel methods of assessing the immune response to and bone forming ability of adult adipose derived mesenchymal stromal cells (ASC). The goals of the research were: 1) evaluate the effect of a bupivacaine mandibular nerve block on blood pressure (BP) and heart rate (HR) in response to surgical stimulation and the need for systemic analgesics postoperatively, 2) quantify …

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The Role Of Monocyte Chemoattractant Protein-1 In Innate Immunity Against Methicillin Resistant Staphylococcus Aureus Pneumonia, Mary Katherine Leissinger Jan 2014

The Role Of Monocyte Chemoattractant Protein-1 In Innate Immunity Against Methicillin Resistant Staphylococcus Aureus Pneumonia, Mary Katherine Leissinger

LSU Master's Theses

Monocyte chemoattractant protein-1 is critical for monocyte recruitment to the lungs in response to bacterial infection. MCP-1 is also essential for protective neutrophil recruitment to the lungs during Escherichia coli and Klebsiella pneumoniae infection. Staphylococcus aureus pneumonia, specifically strain USA300, carries a high morbidity and mortality rate and is an important pathogen in hospital/ventilator and community acquired pneumonia. In the current study, we investigated the role of MCP-1 in pulmonary innate immunity to S. aureus in C57Bl/6, MCP-1-/- and MCP-1 AB blocked mice. As compared to C57Bl/6, MCP-1-/- mice showed increased concentrations of neutrophils in the airways and lung parenchyma …

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Isolation And Molecular Identification Of Avibacterium Paragallinarum In Suspected Cases Of Infectious Coryza, Mahdi Askari Badouei, Avesta Sadrzadeh, Nader Azad, Patrick Blackall, Omid Madadgar, Saeid Charkhkar Jan 2014

Isolation And Molecular Identification Of Avibacterium Paragallinarum In Suspected Cases Of Infectious Coryza, Mahdi Askari Badouei, Avesta Sadrzadeh, Nader Azad, Patrick Blackall, Omid Madadgar, Saeid Charkhkar

Turkish Journal of Veterinary & Animal Sciences

Isolation and identification of Avibacterium paragallinarum, the causative agent of infectious coryza, is considered a challenging task in laboratories with limited specialties. In the present study, 14 commercial layer fowls showing the typical symptoms of infectious coryza were subjected to primary isolation followed by polymerase chain reaction confirmation of suspect colonies (culture-PCR). Direct PCR assays on infraorbital sinus swab samples were also carried out. Thirty-five suspected cases of infectious coryza in commercial broiler chickens were also screened using direct PCR on infraorbital sinus swabs. In culture-PCR, only 1 of the 4 suspected isolates was confirmed as Av. paragallinarum. In comparison, …

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The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem Jan 2014

The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem

Turkish Journal of Veterinary & Animal Sciences

Different levels of pomegranate peel extracted by either water (PEH) or solvent mixture (PEM) were evaluated for their effect on in vitro gas production and ruminal fermentation patterns. Two levels of PEH or PEM (15 and 30 mg total phenolics (TP)/g DM of basal diet) were compared to a control (0) level. Ruminal fermentation parameters such as 24-h partitioning factor (PF_{24}), gas yield (GY_{24}), in vitro organic matter disappearance (IVOMD), metabolizable energy (ME), and microbial protein (MP) were calculated. The addition of PEH or PEM had no effect (P > 0.05) on asymptotic gas production, apparent degraded substrate (ADS), IVOMD, ME, …

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Altered Entrainment To The Day/Night Cycle Attenuates The Daily Rise In Circulating Corticosterone In The Mouse, Patricia J. Sollars, Michael J. Weiser, Andrea E. Kudwa, Jayne R. Bramley, Malcolm D. Ogilvie, Robert L. Spencer, Robert J. Handa, Gary E. Pickard Jan 2014

Altered Entrainment To The Day/Night Cycle Attenuates The Daily Rise In Circulating Corticosterone In The Mouse, Patricia J. Sollars, Michael J. Weiser, Andrea E. Kudwa, Jayne R. Bramley, Malcolm D. Ogilvie, Robert L. Spencer, Robert J. Handa, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) is a circadian oscillator entrained to the day/night cycle via input from the retina. Serotonin (5-HT) afferents to the SCN modulate retinal signals via activation of 5-HT1B receptors, decreasing responsiveness to light. Consequently, 5-HT1B receptor knockout (KO) mice entrain to the day/night cycle with delayed activity onsets. Since circulating corticosterone levels exhibit a robust daily rhythm peaking around activity onset, we asked whether delayed entrainment of activity onsets affects rhythmic corticosterone secretion. Wheel-running activity and plasma corticosterone were monitored in mice housed under several different lighting regimens. Both duration of the light:dark cycle (T …

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Sumoylation Differentially Regulates Sp1 To Control Cell Differentiation, Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi-Zhi Liu, Quan Dong Nguyen, David Wan-Cheng Li Jan 2014

Sumoylation Differentially Regulates Sp1 To Control Cell Differentiation, Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi-Zhi Liu, Quan Dong Nguyen, David Wan-Cheng Li

School of Veterinary and Biomedical Sciences: Faculty Publications

The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 …

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In Situ Detection Of Autoreactive Cd4 T Cells In Brain And Heart Using Major Histocompatibility Complex Class Ii Dextramers, Chandirasegaran Massilamany, Arunakumar Gangaplara, Ting Jia, Christian Elowsky, Qingsheng Li, You Zhou, Jay Reddy Jan 2014

In Situ Detection Of Autoreactive Cd4 T Cells In Brain And Heart Using Major Histocompatibility Complex Class Ii Dextramers, Chandirasegaran Massilamany, Arunakumar Gangaplara, Ting Jia, Christian Elowsky, Qingsheng Li, You Zhou, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

This report demonstrates the use of major histocompatibility complex (MHC) class II dextramers for detection of autoreactive CD4 T cells in situ in myelin proteolipid protein (PLP) 139-151-induced experimental autoimmune encephalomyelitis (EAE) in SJL mice and cardiac myosin heavy chain-α (Myhc) 334-352-induced experimental autoimmune myocarditis (EAM) in A/J mice. Two sets of cocktails of dextramer reagents were used, where dextramers+ cells were analyzed by laser scanning confocal microscope (LSCM): EAE, IAs/PLP 139-151 dextramers (specific)/anti- CD4 and IAs/Theiler’s murine encephalomyelitis virus (TMEV) 70-86 dextramers (control)/anti-CD4; and EAM, IAk/Myhc 334-352 dextramers/ anti-CD4 and IAk/bovine ribonuclease (RNase) 43-56 dextramers (control)/anti-CD4. LSCM analysis of …

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Generation Of Calves Persistently Infected With Hobi-Like Pestivirus And Comparison Of Methods For Detection Of These Persistent Infections, F. V. Bauermann, S. M. Falkenberg, B. Vander Ley, N. Decaro, Bruce W. Brodersen, A. Harmon, B. Hessman, E. F. Flores, J. F. Ridpath Jan 2014

Generation Of Calves Persistently Infected With Hobi-Like Pestivirus And Comparison Of Methods For Detection Of These Persistent Infections, F. V. Bauermann, S. M. Falkenberg, B. Vander Ley, N. Decaro, Bruce W. Brodersen, A. Harmon, B. Hessman, E. F. Flores, J. F. Ridpath

School of Veterinary and Biomedical Sciences: Faculty Publications

The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including bovine viral diarrhea virus (BVDV) and the emerging HoBi-like viruses. Here, colostrum-deprived calves persistently infected with HoBi-like pestivirus (HoBi-like PI calves) were generated and sampled (serum, buffy coat, and ear notches) on the day of birth (DOB) and weekly for 5 consecutive weeks. The samples were subjected to diagnostic tests for BVDV—two reverse transcriptase PCR (RT-PCR) assays, two commercial real-time RT quantitative PCR (RT-qPCR), two antigen capture enzyme-linked immunosorbent assays (ACE), and immunohistochemistry (IHC)—and to HoBi-like virus-specific RT-PCR and RT-qPCR assays. …

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Generation And Screening Of A Comprehensive Mycobacterium Avium Subsp. Paratuberculosis Transposon Mutant Bank, Govardhan Rathnaiah, Elise A. Lamont, N. Beth Harris, Robert J. Fenton, Denise K. Zinniel, Xiaofei Liu, Josh Sotos, Zhengyu Feng, Ayala Livneh-Kol, Nahum Y. Shpigel, Charles J. Czuprynski, Srinand Sreevatsan, Raul G. Barletta Jan 2014

Generation And Screening Of A Comprehensive Mycobacterium Avium Subsp. Paratuberculosis Transposon Mutant Bank, Govardhan Rathnaiah, Elise A. Lamont, N. Beth Harris, Robert J. Fenton, Denise K. Zinniel, Xiaofei Liu, Josh Sotos, Zhengyu Feng, Ayala Livneh-Kol, Nahum Y. Shpigel, Charles J. Czuprynski, Srinand Sreevatsan, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne’s Disease in ruminants. This enteritis has significant economic impact and world wide distribution. Vaccination is one of the most cost effective infectious disease control measures. Unfortunately, current vaccines reduce clinical disease and shedding, but are of limited efficacy and do not provide long-term protective immunity. Several strategies have been followed to mine the MAP genome for virulence determinants that could be applied to vaccine and diagnostic assay developent. In this study, a comprehensive mutant bank of 13,536 MAP K-10 Tn5367 mutants (P>95% )was constructed and screened in …

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Intricacies Of Cardiac Damage In Coxsackievirus B3 Infection: Implications For Therapy, Chandirasegara Massilamany, Arunakumar Gangaplara, Jay Reddy Jan 2014

Intricacies Of Cardiac Damage In Coxsackievirus B3 Infection: Implications For Therapy, Chandirasegara Massilamany, Arunakumar Gangaplara, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Heart disease is the leading cause of death in humans, and myocarditis is one predominant cause of heart failure in young adults. Patients affected with myocarditis can develop dilated cardiomyopathy (DCM), a common reason for heart transplantation, which to date is the only viable option for combatting DCM. Myocarditis/DCM patients show antibodies to coxsackievirus B (CVB)3 and cardiac antigens, suggesting a role for CVB-mediated autoimmunity in the disease pathogenesis; however, a direct causal link remains to be determined clinically. Experimentally, myocarditis can be induced in susceptible strains of mice using the human isolates of CVB3, and the disease pathogenesis of …

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Direct Staining With Major Histocompatibility Complex Class Ii Dextramers Permits Detection Of Antigen- Specific, Autoreactive Cd4 T Cells In Situ, Chandirasegaran Massilamany, Arunakumar Gangaplara, Ting Jia, Christian Elowsky, Guobin Kang, Jean-Jack Riethoven, Qingsheng Li, You Zhou, Jay Reddy Jan 2014

Direct Staining With Major Histocompatibility Complex Class Ii Dextramers Permits Detection Of Antigen- Specific, Autoreactive Cd4 T Cells In Situ, Chandirasegaran Massilamany, Arunakumar Gangaplara, Ting Jia, Christian Elowsky, Guobin Kang, Jean-Jack Riethoven, Qingsheng Li, You Zhou, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

We report here the utility of major histocompatibility complex (MHC) class II dextramers for in situ detection of self-reactive CD4 T cells in two target organs, the brain and heart. We optimized the conditions for in situ detection of antigen-specific CD4 T cells using brain sections obtained from SJL mice immunized with myelin proteolipid protein (PLP) 139–151; the sections were costained with IAs/PLP 139–151 (specific) or Theiler’s murine encephalomyelitis virus (TMEV) 70–86 (control) dextramers and anti-CD4. Analysis of sections by laser scanning confocal microscope revealed detection of cells positive for PLP 139–151 but not for TMEV 70–86 dextramers …

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Influence Of Iron And Aeration On Staphylococcus Aureus Growth, Metabolism, And Transcription, Nagender Ledala, Bo Zhang, Javier Seravalli, Robert Powers, Greg Somerville Jan 2014

Influence Of Iron And Aeration On Staphylococcus Aureus Growth, Metabolism, And Transcription, Nagender Ledala, Bo Zhang, Javier Seravalli, Robert Powers, Greg Somerville

School of Veterinary and Biomedical Sciences: Faculty Publications

Staphylococcus aureus is a prominent nosocomial pathogen and a major cause of biomaterial-associated infections. The success of S. aureus as a pathogen is due in part to its ability to adapt to stressful environments. As an example, the transition from residing in the nares to residing in the blood or deeper tissues is accompanied by changes in the availability of nutrients and elements such as oxygen and iron. As such, nutrients, oxygen, and iron are important determinants of virulence factor synthesis in S. aureus. In addition to influencing virulence factor synthesis, oxygen and iron are critical cofactors in enzymatic and …

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Varestrongylus Eleguneniensis Sp. N. (Nematoda: Protostrongylidae): A Widespread, Multi-Host Lungworm Of Wild North American Ungulates, With An Emended Diagnosis For The Genus And Explorations Of Biogeography, Guilherme G. Verocai, Susan J. Kutz, Manon Simard, Eric P. Hoberg Jan 2014

Varestrongylus Eleguneniensis Sp. N. (Nematoda: Protostrongylidae): A Widespread, Multi-Host Lungworm Of Wild North American Ungulates, With An Emended Diagnosis For The Genus And Explorations Of Biogeography, Guilherme G. Verocai, Susan J. Kutz, Manon Simard, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Background: A putative new species of Varestrongylus has been recently recognized in wild North American ungulates based on the ITS-2 sequences of larvae isolated from feces during a wide geographic survey. No taxonomic description was provided, as adult specimens were not examined. Methods: Lungworm specimens were collected in the terminal bronchioles of muskoxen from Quebec, and a woodland caribou from central Alberta, Canada. The L3 stage was recovered from experimentally infected slugs (Deroceras spp.). Description of specimens was based on comparative morphology and integrated approaches. Molecular identity was determined by PCR and sequencing of the ITS-2 region of …

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