Pharmacy and Pharmaceutical Sciences Commons^™

Production And Characterization Of Monoclonal Antibodies Against Botulinum Toxin Produced In Nicotiana Benthamiana, Kornchanok Sangprasat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Botulism is an uncommon but serious neurological condition caused by the botulinum toxin produced by the gram-positive bacterium named Clostridium botulinum. Common symptoms of botulism include muscle cramps, nausea, vomiting, diarrhea, difficulty swallowing, respiratory failure, muscle weakness, and potentially death. Currently, two types of antitoxin are used: equine-derived heptavalent antitoxin and human-derived immunoglobulin (BabyBIG®). However, treatment with heptavalent antitoxin may lead to hypersensitivity reactions, while BabyBIG®, which is produced in low yield, is used only for children. This research focuses on developing three anti-BoNT monoclonal antibodies (mAbs), 1B18, C25, and M2, which will be produced using Nicotiana benthamiana as a …

ผลในระดับห้องปฏิบัติการของไฮโดรเจลคาร์บอกซีเมทิลเซลลูโลสและเซริซินที่บรรจุกรดแกลลิกในเซลล์ไฟโบบลาสต์และเคราติโนไซท์ของมนุษย์, ณภัทร์ แหลมคม

Chulalongkorn University Theses and Dissertations (Chula ETD)

กรดแกลลิกเป็นสารกลุ่มกรดฟีนอลิก มีฤทธิ์ทางเภสัชวิทยาที่ดีแต่มีข้อจำกัดเนื่องจากจะถูกดูดซึมและถูกกำจัดออกจากร่างกายอย่างรวดเร็วหลังจากเข้าสู่กระแสเลือดรวมถึงมีความคงตัวต่ำ ปัจจุบันจึงมีการศึกษาระบบนำส่งยารูปแบบต่างๆ รวมถึงไฮโดรเจล เพื่อนำมาประยุกต์ใช้ในการนำส่งกรดแกลลิก งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาการควบคุมการปลดปล่อยกรดแกลลิกจากไฮโดรเจลผสมระหว่างคาร์บอกซีเมทิลเซลลูโลสและเซริซิน โครงสร้างสัณฐานของไฮโดรเจลที่ถูกเตรียมจากคาร์บอกซีเมทิลเซลลูโลสและเซริซิน ที่อัตราส่วน 1:1 และ 1:2 โดยปริมาตร และเชื่อมขวางด้วยสารละลายกลูตารัลดีไฮด์ 4 เปอร์เซ็นต์โดยปริมาตร และขึ้นรูปด้วยวิธีแช่แข็ง-ละลาย โดยมีรูพรุนสม่ำเสมอและเชื่อมโยงกันเป็นโครงร่างสามมิติ จากผลค่าโมดูลัสกักเก็บ พบว่าค่าโมดูลัสกักเก็บของไฮโดรเจลมีค่าสูงขึ้นเมื่อสัดส่วนของเซริซินเพิ่มขึ้น ผลการย่อยสลายได้ทางชีวภาพพบว่าอัตราการย่อยสลายลดลงเมื่อเพิ่มสัดส่วนของเซริซิน ชี้ให้เห็นว่าสัดส่วนของเซริซินส่งผลต่อความแข็งแรง ความยืดหยุ่น และความคงรูปของไฮโดรเจล และเมื่อนำไฮโดรเจลไปดูดซับกรดแกลลิกเพื่อศึกษาการควบคุมการปลดปล่อย พบว่ากรดแกลลิกถูกดูดซับบนไฮโดรเจลในอัตราการดูดซับที่สูงกว่าร้อยละ 80 และพบว่ากรดแกลลิกถูกปลดปล่อยออกมาอย่างรวดเร็วในระยะเวลา 15 นาที ถึง 2 ชั่วโมงแรก จากนั้นจะค่อย ๆ ปลดปล่อยออกมาลดลงเรื่อย ๆ โดยมีการปลดปล่อยอยู่ในช่วงร้อยละ 65 รวมทั้งพบว่ากรดแกลลิกถูกปลดปล่อยในสภาวะที่เป็นเบส ได้มากกว่าในสภาวะที่เป็นกลางและกรด โดยกลไกการปลดปล่อยกรดแกลลิกสามารถอธิบายได้ด้วยสมการเภสัชจลนศาสตร์แบบ Kopcha ชี้ให้เห็นว่าการปลดปล่อยอาศัยการแพร่และการย่อยสลายของไฮโดรเจล และเมื่อศึกษาการบวมน้ำของไฮโดรเจลพบว่าสอดคล้องกับอัตราการปลดปล่อยของกรดแกลลิก เมื่อทดสอบความเข้ากันได้ทางชีวภาพต่อเซลล์ในระดับห้องปฏิบัติการพบว่าไฮโดรเจลคาร์บอกซีเมทิลเซลลูโลสและเซริซินที่บรรจุกรดแกลลิกไม่มีความเป็นพิษต่อเซลล์ไฟโบบลาสต์และเคราติโนไซท์ของมนุษย์ และไม่มีผลกระตุ้นกระบวนการอักเสบภายในเซลล์แมคโครฟาจของหนูเมาส์ และเมื่อนำไฮโดรเจลที่เตรียมขึ้นไปทดสอบการยับยั้งการสร้างอนุมูลอิสระในเซลล์ไฟโบบลาสต์และเคราติโนไซท์ของมนุษย์ พบว่ากรดแกลลิกที่ถูกปลดปล่อยจากไฮโดรเจลสามารถยับยั้งการสร้างอนุมูลอิสระได้ จากผลการวิจัยสามารถสรุปได้ว่า ไฮโดรเจลคาร์บอกซีเมทิลเซลลูโลสและเซริซินที่เตรียมขึ้นสามารถนำไปประยุกต์ใช้ในการดูดซับและควบคุมการปลดปล่อยกรดแกลลิกที่มีผลต่อการยับยั้งการสร้างอนุมูลอิสระในเซลล์ไฟโบบลาสต์และเคราติโนไซท์ของมนุษย์ในระดับห้องปฏิบัติการได้อย่างมีประสิทธิภาพโดยที่ไม่ทำลายฤทธิ์ทางเภสัชวิทยาของกรดแกลลิก

Impact Of A Culturally-Grounded And Interprofessional Approach To Managing Latino/Hispanic Patients At Risk For Or Diagnosed With Type 2 Diabetes Mellitus: A Pilot Study, Jahnavi Yalamanchili

University of the Pacific Theses and Dissertations

Background: Despite significant progress in diabetes research, there remain significant health disparities between Hispanic/Latino patients in the United States, particularly those with lower socioeconomic status, compared to non-Hispanic white patients. Previous studies have identified potential reasons behind these disparities, including the need for a multifaceted approach that addresses social determinants of health (SDOH) faced by patients. Social cognitive theory (SCT) stipulates that interventions that promote self-management by improving knowledge, self-efficacy, and problem-solving skills can be successful in improving health outcomes. However, there is a paucity of studies that have evaluated an interprofessional approach to providing culturally relevant care, in particular …

Elaboration Of Diquinanes To Access Trifunctional Angular Triquinanes And Designed Dna Polymerase Α Inhibitors, Spencer M. Webber

University of the Pacific Theses and Dissertations

Natural products are of great significance to the pharmaceutical industry in the development of new drugs. Diquinane or bicyclo[3.3.0]octane is a conspicuous structural unit existing in the carbo-frameworks of a wide range of natural products such as alkaloids and terpenoids. These diquinane-containing molecules not only exhibit intriguing architectures, but also showcase a broad spectrum of significant bioactivities, which draw widespread attention from the global synthetic community. A more specific group of compounds containing a diquinane moiety with one extra 5-membered ring are called the angular triquinanes.In this work, we have developed a general synthetic scheme to the angular triquinanes. We …

Impact Of Drug-Polymer Interactions On Stability In Simvastatin-Based Amorphous Solid Dispersions, Charina Lampa

University of the Pacific Theses and Dissertations

This study aimed to investigate the drug-polymer interactions between simvastatin (SIM) and three polymers [PVP-VA, HPMC-AS, and Soluplus® (SOL)] in amorphous solid dispersions (ASDs) prepared by hot melt extrusion (HME), and to understand the implications on physical stability. ASDs are of significant interest within the pharmaceutical industry for improving drug bioavailability. However, the amorphous nature of a drug in an ASD presents physical stability challenges. Utilizing novel applications of accessible tools such as ATR-FTIR or DSC aid in understanding the mechanisms of stabilization which are critical to the rational design of ASDs. ASDs were prepared using HME and were characterized …

Perspectives On Using Pharmacogenomics To Guide Tobacco Cessation: Survey Results From An American Indian Community, Daniela Mariana Wall

Graduate Student Theses, Dissertations, & Professional Papers

Pharmacogenomics research has predominantly focused on populations of European ancestry, limiting the application for diverse populations such as American Indian and Alaska Native (AIAN) communities. Our community-centric study aims to understand perspectives of utilizing pharmacogenomics to guide tobacco cessation in an AIAN community using a survey with qualitative and quantitative components. We assessed participant (n=273) tobacco usage and cessation history, pharmacogenomics knowledge, and perceptions of utilizing pharmacogenomics in the context of tobacco cessation. We found that the majority of participants (92%) were aware of risks associated with tobacco usage and believe it to be a problem within their community (76%). …

Perspectives Of Utilizing Pharmacogenomics To Inform Tobacco Cessation: Survey Results From An American Indian Community, Madeline Lousie Davies

Graduate Student Theses, Dissertations, & Professional Papers

Pharmacogenomics research has predominantly focused on populations of European ancestry, limiting the application for diverse populations such as American Indian and Alaska Native (AIAN) communities. Our community-centric study aims to understand perspectives of utilizing pharmacogenomics to guide tobacco cessation in an AIAN community using a survey with qualitative and quantitative components. We assessed participant (n=273) tobacco usage and cessation history, pharmacogenomics knowledge, and perceptions of utilizing pharmacogenomics in the context of tobacco cessation. We found that the majority of participants (92%) were aware of risks associated with tobacco usage and believe it to be a problem within their community (76%). …

Treatment Patterns And Clinical And Economic Burden In Patients With Merkel Cell Carcinoma: Statistical And Machine Learning Approaches, Yves Mbous

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although considered a rare form of nonmelanoma skin cancer, Merkel Cell Carcinoma incidence rate has increased to 0.74 per 100,000 and projections expect this number to steadily rise over the next decades and to reach an average of 3,285 cases per year in 2025. MCC incidence increases significantly with age. Among those aged 40-44, it is 0.44, it rises to 1.0/100,000 in the 60-64 age group and soars to 9.8/100,000 among those ≥85 years and above. Due to the small number of cases, MCC is often managed using institutional practices or provider preferences. Formal clinical trials that enroll early stage …

Intervention To Reduce Tobacco Use Among Prostate Cancer Patient And Informal Caregiver Dyads, Trupti Dhumal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prostate cancer is the leading cancer in men. Continued tobacco use after prostate cancer diagnosis may affect a patient’s treatment outcomes, quality of life, and mortality. Despite the potential negative outcomes, 10-20% of prostate cancer patients continue to use tobacco after their cancer diagnosis. Considering risks associated with continued tobacco use, there is a lack of evidence-based tobacco cessation strategies to address tobacco cessation specifically in prostate cancer. Given the increasing prevalence of prostate cancer, the ongoing difficulty in tobacco cessation, and the limited evidence-based tobacco cessation interventions in the literature, it is important to prioritize reducing tobacco use in …

Development Of Cannabis Extract Active Coating On Granules With Taste Masking Polymer Via Fluidized Bed, Samuel Wirajaya Prawinata

Chulalongkorn University Theses and Dissertations (Chula ETD)

Abstract Cannabis extract, one of the herbal medicine products on the national list of essential medicines, offers various therapeutic benefits; however, it has unpleasant taste, and the products are available as liquid preparation which can significantly reduce patient compliance and drug effectiveness. The objectives of this study were to develop film formulations and characterizations composed of tetrahydrocannabinol and taste-masking polymers and develop tetrahydrocannabinol-coated granules via a fluidized bed-coating. Eudragit E100 (E100) and Kollidon VA64 (KVA64) were used as taste-masking polymers. Plasticizers with different chemical structures, including polyethylene glycol (PEG-400), triacetin, and diethyl phthalate, were used to modify various film characteristics. …

Identification Of Carpaine In Carica Papaya Leaf Products Using Direct Analysis In Real Time Mass Spectrometry (Dart-Ms), Piyawadi Khaoiam

Chulalongkorn University Theses and Dissertations (Chula ETD)

Carpaine, a major alkaloid present in Carica papaya leaves, has been shown to increase platelet counts in patients suffering from thrombocytopenia. Numerous commercial papaya leaf products are available, but few provide proper bioactive ingredient information. We present herein a technique for rapid screening of carpaine in these products using DART-MS. The results indicate that carpaine was detected in various forms (powder, solution) of papaya leaves. Its presence was confirmed by examining the mass pattern when conducted on a standard solution at both low and high voltages (+10 V and +90 V), using MS1 and MS2 data obtained from LC-QTOF-MS/MS. The …

Targeting The Renin-Angiotensin System (Ras) For Neuropsychiatric Disorders, Aline Silva De Miranda, Danielle S Macedo, Natalia P Rocha, Antonio L Teixeira

Faculty, Staff and Student Publications

BACKGROUND: Neuropsychiatric disorders, such as mood disorders, schizophrenia, and Alzheimer's disease (AD) and related dementias, are associated to significant morbidity and mortality worldwide. The pathophysiological mechanisms of neuropsychiatric disorders remain to be fully elucidated, which has hampered the development of effective therapies. The Renin Angiotensin System (RAS) is classically viewed as a key regulator of cardiovascular and renal homeostasis. The discovery that RAS components are expressed in the brain pointed out a potential role for this system in central nervous system (CNS) pathologies. The understanding of RAS involvement in the pathogenesis of neuropsychiatric disorders may contribute to identifying novel therapeutic …

Addressing Inappropriate Polypharmacy: Investigating Perceived Facilitators And Barriers To U.S. Pharmacists' Ability To Recommend Deprescribing, Susanne Steiner

Theses and Dissertations

While the concurrent use of multiple prescriptions (polypharmacy) is common for those with chronic conditions, professional ownership is lacking when it comes to tapering or discontinuing potentially inappropriate medications. Our growing elderly population is especially vulnerable to negative risks associated with polypharmacy. Pharmacists are highly educated and skilled healthcare professionals working as critical touchpoints with patients; yet they are not empowered to deprescribe potentially inappropriate medications directly. This qualitative research study examined the perceived barriers and facilitators pharmacists encounter when pursuing deprescribing recommendations and initiatives with prescribers, healthcare staff, patients, and caregivers. Findings from this study revealed three key implications …

Introducing Biosimilar Competition For Cell And Gene Therapy Products, Brian Canter, Sabine Sussman, Stephen Colvill, Nitzan Arad, Elizabeth Staton, Arti Rai

Faculty Scholarship

This article provides an early analysis of the potential for creating future biosimilar competition for cell and gene therapies (CGTs) to lower prices and improve patient access, building on a unique set of interviews with relevant experts. Our discussion addressed regulatory, manufacturing, intellectual property, and market size challenges. Due to CGTs’ complexity, meeting the regulatory requirement of ‘high similarity with no clinically meaningful differences’ will be difficult. Gene therapies are likely better candidates for biosimilar development than cell therapies. Biosimilarity should be met when gene therapy biosimilars contain the same genetic sequence as a reference product, and the variability in …

2,3,4 Trihydroxybenzoic Acid, A Plant Secondary Metabolite Inhibits Cancer Cell Growth, Mina Akter

Electronic Theses and Dissertations

Nature offers many sources such as fruits, beverage crops, vegetables, herbs, spices, tea, dark chocolate, and wine, that are rich in hydroxybenzoic acids (HBAs). They are a prominent phenolic acids with excellent biochemical and antioxidant properties. Due to the vast dietary health benefits of HBAs such as anti-inflammatory, anti-allergenic, immunoregulatory, antimicrobial, antithrombotic, antidiabetic, anticancer properties and cardioprotective capabilities, they are attracting the attention of physicians and researchers alike to understand their mechanisms of action. It is anticipated that the understanding of the role of HBAs for their greater use in diet/supplements is beneficial to reverse common illnesses such as inflammation, …

Implementation Of Medication Inventory Management Solution At A Critical Access Hospital, Justin Donat, Christopher Adams

Books, Presentations, Posters, Etc.

Abstract: Accurately monitoring pharmacy inventory in real-time is vital to providing safe and cost-effective medical care at hospitals of any size. At smaller critical access hospitals, in particular, effective inventory management requires solutions to unique problems posed by floorspace, budget, and workflow limitations. In 2022, Providence Health System (PHS) began systemwide implementation of both a standardized inventory management solution (IMS) and an AI-assisted medication ordering software (MOS). By initiation of this project, larger hospitals in the system had already implemented the IMS and MOS at their inpatient pharmacies to mixed success. In May of 2024, Providence Hood River Memorial Hospital …

Review Of Current Dual-Antiplatelet And Triple Therapy Prescribing Practices At Providence Medical Group, Gina Kim, Christine Doran, Lauren Powell

Books, Presentations, Posters, Etc.

Abstract: Approximately 10% of patients with recent percutaneous intervention (PCI) have concomitant atrial fibrillation (AF) and others may have concomitant venous thromboembolism (VTE). This makes choosing the optimal antithrombotic regimen challenging. The ACC published a 2020 decision pathway to guide safe and evidence-based use of antiplatelet and anticoagulant combinations for certain patients. These situations include patients with prior AF on anticoagulation and the need for PCI, patients with new-onset AF requiring anticoagulation in a patient already on antiplatelet therapy for coronary artery disease (CAD), patients with prior VTE on anticoagulation and the need for PCI, and patients with new or …

A Real-World Disproportionality Analysis Of Everolimus: Data Mining Of The Public Version Of Fda Adverse Event Reporting System, Bin Zhao, Yumei Fu, Shichao Cui, Xiangning Chen, Shu Liu, Lan Luo

Faculty, Staff and Student Publications

Background: Everolimus is an inhibitor of the mammalian target of rapamycin and is used to treat various tumors. The presented study aimed to evaluate the Everolimus-associated adverse events (AEs) through data mining of the US Food and Drug Administration Adverse Event Reporting System (FAERS).

Methods: The AE records were selected by searching the FDA Adverse Event Reporting System database from the first quarter of 2009 to the first quarter of 2022. Potential adverse event signals were mined using the disproportionality analysis, including reporting odds ratio the proportional reporting ratio the Bayesian confidence propagation neural network and the empirical Bayes geometric …

Tracing Antibacterial Compounds From Kaledang (Artocarpus Lanceifolius Roxb.) Stem Bark, Hamsidar Hasan, Tety Tuloli, Syamsul Bahri, La Ode Aman

Pharmaceutical Sciences and Research

Artocarpus lanceifolius Roxb. or kaledang is a species of the mulberry family, Moraceae. Several extracts from its family have been reported to have antibacterial activity. Thus, it is necessary to determine compounds that have nature as antibacterial to be raw materials for antibacterial herbal medicines. The purpose of the study was to trace compounds that have antibacterial activity from Artocarpus lanceifolius stem bark. The study method was extraction by graded maceration, fractionation with column vacuum chromatography (CVC), isolation by radial chromatography, determination of antibacterial activity by agar diffusion method using the Gram positive and Gram negative bacteria. The characterization …

Antimicrobial Activity Of Crude Fractions Of Gossypium Barbadense Leaves And Isolation Of Stigmasterol, Maryam Saddiq, Ahmed Salisu, Kamaluddeen Kabir, Yusuf Hassan

Pharmaceutical Sciences and Research

Plants have been used in traditional medicine in different parts of the world to treat several diseases. Many studies have confirmed their medicinal values, which have led to the isolation and characterization of various potential drug candidates. This study focuses on the antimicrobial activity of crude fractions of Gossypium barbadense leaves and the isolation of active compound. The pulverized plant leave was soaked in ethanol (98% v/v), and a solvent-based fractionation was carried out using n-hexane, chloroform, ethyl acetate, and ethanol. The obtained crude fractions were screened for antimicrobial activity against four (4) clinical isolates: Escherichia coli, Pseudomonas …

Smart Missions That Enable Innovation In High-Risk Environments., Ed Hoffman

Level 3

This article is a lightly edited version of Ed Hoffman’s talk on to the 01-June-2023 seminar hosted by the PRST entitled ICH Q9(R1): The Next Frontier – An audience with international thought leaders exploring how the effective use of knowledge can enhance QRM outcomes to benefit the patient. Ed’s remarks are focused on the future of work, and the needs for relationships, storytelling, and understanding the mission to get the best out of our collective intelligence and navigate the future of work.

Subchronic Toxicity Study Of Sterculia Rubiginosa Zoll. Ex Miq. Leaves Extract, Rini Prastiwi, Ester Hidayati, Riska Anggraini, Cut Mauliza, Ita Anggraini, Ema Dewanti

Pharmaceutical Sciences and Research

Ki hampelas (Sterculia rubiginosa Zoll. Ex Miq.) is traditionally used as an antiasthma. It also reported has antioxidant and nephroprotective activity. This study was conducted to evaluate the subchronic toxicity of the leaf extract of ki hampelas. The extract was orally administered to male and female Sprague-Dawley rats at doses of 50, 200, and 400 mg/kg bodyweight (BW) per day for 28 days. The rats were divided into four groups, consist of consist of normal group (Na CMC 0.5%), dose 1 (50 mg/kg BW), dose 2 (200 mg/kg BW), and dose 3 (400 mg/ kg BW) of extract. The …

Two-Pore Channel-2 And Inositol Trisphosphate Receptors Coordinate Ca2+ Signals Between Lysosomes And The Endoplasmic Reticulum, Yu Yuan, Vikas Arige, Ryo Saito, Qianru Mu, Gabriela C. Brailoiu, Gustavo J.S. Pereira, Stephen R. Bolsover, Marco Keller, Franz Bracher, Christian Grimm, Eugen Brailoiu, Jonathan S. Marchant, David I. Yule, Sandip Patel

College of Pharmacy Faculty Papers

Lysosomes and the endoplasmic reticulum (ER) are Ca2+ stores mobilized by the second messengers NAADP and IP3, respectively. Here, we establish Ca2+ signals between the two sources as fundamental building blocks that couple local release to global changes in Ca2+. Cell-wide Ca2+ signals evoked by activation of endogenous NAADP-sensitive channels on lysosomes comprise both local and global components and exhibit a major dependence on ER Ca2+ despite their lysosomal origin. Knockout of ER IP3 receptor channels delays these signals, whereas expression of lysosomal TPC2 channels accelerates them. High-resolution Ca2+ imaging reveals elementary events upon TPC2 opening and signals coupled to …

Colon Targeted Delivery Dosage Forms For Probiotics: A Review, Gede Anom Anjasmara, Pietradewi Hartrianti, Raditya Iswandana

Pharmaceutical Sciences and Research

Encapsulation was a promising method for protecting probiotics from extreme conditions during their passage through the gastrointestinal tract and delivering probiotics to specific sites in the colon for colonization. Various dosage forms have been used in recent years to encapsulate probiotics to maintain cell viability during processing, storage, and through the digestive tract to provide health benefits. However, research related to the encapsulation of probiotics as the dosage forms for colon-targeted delivery systems was still quite limited to conventional dosage forms due to the sensitivity of probiotics to extreme conditions during the process. This review focuses on various types of …

Molecular Docking Analysis Of Acanthus Ilicifolius Compounds Toward Cul4b-Ddb1-Ahr-Erα Complex Protein For Antiosteoporosis Discovery, Binar Asrining Dhiani, Sarmoko Sarmoko, Retno Wahyuningrum, Akbar Yulianto

Pharmaceutical Sciences and Research

Osteoporosis represents a significant global public health issue, particularly among the aging population. Its incidence reaches 18.3% of the total population, with the highest prevalence observed in elderly postmenopausal women. A key factor in osteoporosis is the decreased expression level of estrogen receptor alpha (ERα), attributed to its degradation by the ubiquitin ligase protein complex Cullin4B (CUL4B), DNA damage binding 1 (DDB1), and aryl hydrocarbon receptor (AhR), collectively known as CUL4BAhR. Acanthus ilicifolius L contains compounds exhibiting antiosteoporosis activity, primarily by inhibiting osteoclastogenesis via RANKL. However, no reports exist of antiosteoporosis agents that act by inhibiting ERα degradation via CUL4BAhR. …

Exploring Rosella (Hibiscus Sabdariffa L.) Calyx Extracts As Natural Dye And Antioxidants In Lip Cream Product: Formulation And Evaluation, Donna Maretta Ariestanti, Abdul Mun'im, Joshita Djajadisastra, Riska Amalia Putri Hutami, Redhalfi Fadhila, Richard Johari James

Pharmaceutical Sciences and Research

Rosella, also known as Hibiscus sabdariffa L., is a plant that contains anthocyanin compounds in its calyx, which are known for their antioxidant properties. These compounds give the plant a captivating red color and can be used as natural dyes. This study aimed to exploit anthocyanins in rosella calyx as natural dyes and antioxidants in lip cream product. The formulations employed extracts from rosella calyx, a viscous extract from maceration in 96% ethanol (F1) and a commercially available powdered rosella extract (F2). Evaluation on the formulations demonstrated that both F1 and F2 effectively maintained the color integrity of the lip …

The Potential Of Herbal Combinations As Adjunctive Therapy For Standard Care Of Covid-19 Patients: A Lesson Learned From Indonesia, Anna Rozaliyani, Satria Pratama, Findra Setianingrum, Fenny Yunita, C. Martin Rumende

Pharmaceutical Sciences and Research

The pandemic of novel coronavirus disease (COVID-19) has become a global disaster that occurred between the end of 2019 and 2023. It caused immeasurable damage to almost all aspects of human life. The disease has opened a lot of new perspectives on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) origins and pathogenesis, the nature of the virus, the potential for new treatments, and new policies to overcome this pandemic. This review aims to know the potential of plants that are beneficial to combat the diseases, including immunomodulators in the management of COVID-19. Studies reporting COVID-19 with the keywords SARS-CoV-2, COVID-19, …

Antioxidant Activities Of Roja Herbal Tea: Combination Of Rosella Flower (Hibiscus Sabdariffa L.) And Ginger Rhizome (Zingiber Officinale Rosc.), Wahyu Widowati, Teresa Liliana Wargasetia, Teddy Marcus Zakaria, Michael Sebastian Gunadi, Nathanael Halim, Sherly Santiadi, Hanna Sari Widya Kusuma, Nida Firyal Fauziyah

Pharmaceutical Sciences and Research

Herbal teas and plant-based medicines have been widely used worldwide for their therapeutic and healing qualities. Rosella (Hibiscus sabdariffa L.) has various benefits in preventing digestive problems, liver disease, fever, and others. Ginger (Zingiber officinale Rosc.) or known as jahe in Indonesia is believed to benefit numerous clinical conditions linked to oxidative stress, including pancreatitis, hypertension, diabetic kidney disease, Alzheimer’s disease, and tumor development. They have been utilized in traditional medical practice to treat conditions such as fever, nausea, and headaches. In this study, we aimed to determine the antioxidant activities of rosella flower tea, ginger tea, and …

Study Of Anti-Hyperuricemia, Anti-Inflammatory, And Anti-Nociceptive Effects Of Hylocereus Undatus Stem Bark Extract In Animal Models, Tri Wahyuni, Sartika Harka Putri, Anton Bahtiar

Pharmaceutical Sciences and Research

Dragon fruit, known as Hylocereus undantus (HU), has traditionally been utilized in Banyuwangi, East Java, Indonesia, for addressing gout and rheumatism. Despite its empirical use, there is a lack of reported pharmacological research on this plant. This study aimed to explore the properties of HU extract in treating hyperuricemia, inflammation, and pain. Adenine-potassium oxonate-induced mice were employed to evaluate anti-hyperuricemia activity, measuring uric acid, blood urea nitrogen (BUN), and creatinine serum levels. Carrageenan-induced paw edema in rats was used to assess anti-inflammatory activity, while acetic acid-induced abdominal writhing and hot plate tests were conducted to investigate anti-nociceptive effects. The results …

Menakar Kesiapan Puskesmas Dalam Penuntasan Penanganan Penyakit: Analisa Data Riset Fasilitas Kesehatan 2019, Harimat Hendarwan, Suparmi Suparmi

Jurnal Ekonomi Kesehatan Indonesia

The completion of disease treatment at the health center was important for payment of Capitation Based on Fulfillment of Service Commitment (PCFSC/KBPKP) and the Non-Specialist Case Outpatient Referral (NSOR/RRNS) was one of the key indicators. This study aimed to assess public health center readiness in the completion of disease treatment for those stipulated in Ministerial Decree number 514 of the year 2015. This study used Health Facility Research (Rifaskes) data conducted in 2019 with a total of 9831 health centers as observations. Analysis was conducted by comparing the availability of equipment, laboratories, and medicines with existing regulations. Health center readiness …