Medical Sciences Commons^™

ความชุกของประชากรที่มีการเมตาบอลิสมยาไม่ดี จากโพลิมอร์ฟิซัมไซโตโครมพีสี่ห้าศูนย์เอนไซม์ซิปสองซีสิบเก้า ในผู้ป่วยโรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมองในโรงพยาบาลจุฬาลงกรณ์, ธนกฤต พงพิทักษ์เมธา

Chulalongkorn University Theses and Dissertations (Chula ETD)

บทนำ โรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมอง (symptomatic intracranial atherosclerotic disease; sICAD) เป็นโรคสมองขาดเลือดที่พบได้บ่อยมากเป็นอันดับที่ 2 ของผู้ป่วยโรคสมองขาดเลือดในคนไทย และมีอัตราการเป็นซ้ำที่สูง ประมาณ ร้อยละ 15 ในปีแรก เมื่อเทียบกับกลุ่มอื่นที่ประมาณ ร้อยละ 5% ในปีแรก ทำให้งานวิจัยและแนวทางการรักษาในระดับชาติและนานาชาติ จึงแนะนำให้ใช้ยาต้านเกล็ดเลือดสองชนิดควบคู่กันระยะสั้น (short term dual antiplatelet; DAPT) โดยเฉพาะสูตรที่มียาโคพิโดเกรล (clopidogrel) เป็นส่วนผสม เพื่อป้องกันการเกิดโรคหลอดเลือดสมองซ้ำต่อไป อย่างไรก็ตามยา clopidogrel นั้นจำเป็นที่จะต้องเปลี่ยนแปลงโดยผ่านเอนไซม์ไซโตโครมพีสี่ห้าศูนย์ เอนไซม์ซิปสองซีสิบเก้า (CYP2C19) เพื่อให้ยาอยู่ในสภาพออกฤทธิ์และป้องกันโรคต่อไป จากการศึกษาก่อนหน้าพบว่าความชุกของประชากรที่มีการเมตาบอลิสมยาไม่ดี (poor metabolizers; PM) ของเอนไซม์ CYP2C19 ในประชากรไทยมีสัดส่วนจากมากแตกต่างจากประชากรชาติตะวันตก อย่างไรก็ตามจากการศึกษาในอดีตเกี่ยวกับของการเปลี่ยนแปลงทางเภสัชพันธุศาสตร์ของเอนไซม์ CYP2C19 ต่อผลการรักษาทางคลินิกในผู้ป่วยโรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมอง ยังมีอยู่อย่างจำกัดและผลไม่สอดคล้องกันในหลายงานวิจัย วัตถุประสงค์ วัตถุประสงค์หลักของงานวิจัย (primary objective) คือศึกษาความชุกของประชากรที่มีการเมตาบอลิสมยาไม่ดีของเอนไซม์ CYP2C19 ผู้ป่วยโรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมองในโรงพยาบาลจุฬาลงกรณ์ วัตถุประสงค์รองของงานวิจัย (secondary objectives) คือเพื่อศึกษาผลของการเสียการทำงานของ CYP2C19 allele (LOF allele status) ต่ออุบัติการณ์การเกิดโรคหลอดเลือดสมองตีบซ้ำ (recurrent stroke) การเกิดภาวะแทรกซ้อนทางหัวใจที่รุนแรง (major adverse cardiac event; MACE) การเกิดเลือดออก (bleeding event) ที่ 90 วันหลังเกิดโรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมอง วิธีการทำวิจัย เก็บข้อมูลผู้ป่วยจากการศึกษาไปข้างหน้าเชิงพรรณนา (ongoing prospective study) ตั้งแต่เดือนกรกฎาคม พ.ศ. 2565 โดยเก็บผู้ป่วยอายุมากกว่าหรือเท่ากับ 40 ปี ที่มีอาการ อาการแสดง ผลตรวจทางรังสีวิทยาที่เข้าได้กับโรคสมองขาดเลือดที่มีอาการเนื่องจากการแข็งหลอดเลือดแดงในสมอง (หลอดเลือดตีบ 50-99%) และมีผลตรวจทางรังสีวิทยามีผลการตรวจหลอดเลือดสมองทางรังสีวิทยายืนยัน โดยผู้วิจัยจะได้เก็บข้อมูลทางคลินิก ข้อมูลทางยา การตรวจทางห้องปฏิบัติการ การตรวจทางรังสีวิทยา จากการทบทวนข้อมูลในเวชระเบียนระหว่างมานอนโรงพยาบาลและที่ตรวจติดตาม …

The Comparison Between Alternative And Standard Methods For Quality Control Of Diphtheria Toxoid In Thai-Gpo’S Dtp And Dt Vaccines (Adsorbed), Wanlop Leeden

Chulalongkorn University Theses and Dissertations (Chula ETD)

In response to the country's vaccine security policy and to make Thailand self-reliant in vaccine supply, the Government Pharmaceutical Organization has been successfully established he the production process of dT and DTP vaccine (Adsorbed). According to WHO recommendation, the quality control scheme for dT, DTP vaccines (adsorbed) includes identity test, toxicity and reversion to toxicity test, potency test, and antigen content and degree of absorption. Therefore, it is of interest to study and develop appropriate methods for use in quality control and for inclusion in the product registration application process. The comparison study of the quality testing methods can categorized …

Efficiency Of Mrna Vaccine Against House Dust Mite Allergy In Mouse Model, Chanatip Thanasarnthungcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Through the capacity to mediate Th1-biased immune responses and the induction of neutralizing antibodies, the mRNA-lipid nanoparticle (LNP) platform could represent an innovative approach for shorter, safer, and more effective treatment of house dust mite (HDM) allergy. Our previous study showed that nucleoside-modified mRNA encoding recombinant forms of HDM allergens Der p 1 and Der p 2 (ProDer p 1 and Der p 2 K96A) and formulated with ionizable LNP, induced Th1-biased immune responses and potent specific blocking IgG responses, while being non-reactogenic in sensitized mice. This present study evaluated the immunotherapeutic efficacy of HDM-specific mRNA-LNP-based AIT in a mouse …

A Descriptive And Visual Analysis Of The North Coast Fall Prevention Survey Data, Mishell Lopez

Cal Poly Humboldt theses and projects

Older adults over 65 will double from 52.4 million in 2018 to 94.7 million in 2060. Among this aging group, falls affect one out of four older adults and are a critical economic concern for our American society, with yearly costs of $50 billion. In older adults, falling can result in negative consequences such as fear of falling, injuries, mobility restrictions, and loss of independence. California has one of the highest costs per fall ($4 billion annually) and a high percentage of adults who have fallen (30.6%) compared to the nation. Moreover, two of California’s northwest rural counties, Del Norte, …

Social Behavioral Impairments In Syngap1-Related Intellectual Disability, Hajer Naveed, Maria Mccormack, J Lloyd Holder

Duncan NRI Faculty and Staff Publications

Introduction: Developmental synaptopathies are neurodevelopmental disorders caused by genetic mutations disrupting the development and function of neuronal synapses.

Methods: We administered the validated Social Responsiveness Scale, Second Edition (SRS-2) to investigate the phenotypic presentation of social-behavioral impairments for the developmental synaptopathy-SYNGAP1-related Intellectual Disability (SYNGAP1-ID) (n = 32) compared with a phenotypically similar disorder Phelan-McDermid syndrome (PMD) (n = 27) and healthy controls (n = 43). A short form SRS-2 analysis (n = 85) was also conducted.

Results: Both SYNGAP1-ID and PMD had significantly elevated total and subcategory T-scores, with no significant score differences between SYNGAP1 …

Upregulation Of The Escrt Pathway And Multivesicular Bodies Accelerates Degradation Of Proteins Associated With Neurodegeneration, Ron Benyair, Sai Srinivas Panapakkam Giridharan, Pilar Rivero-Ríos, Junya Hasegawa, Emily Bristow, Eeva-Liisa Eskelinen, Merav D Shmueli, Vered Fishbain-Yoskovitz, Yifat Merbl, Lisa M Sharkey, Henry L Paulson, Phyllis I Hanson, Samarjit Patnaik, Ismael Al-Ramahi, Juan Botas, Juan Marugan, Lois S Weisman

Duncan NRI Faculty and Staff Publications

Many neurodegenerative diseases, including Huntington’s disease (HD) and Alzheimer’s disease (AD), occur due to an accumulation of aggregation-prone proteins, which results in neuronal death. Studies in animal and cell models show that reducing the levels of these proteins mitigates disease phenotypes. We previously reported a small molecule, NCT-504, which reduces cellular levels of mutant huntingtin (mHTT) in patient fibroblasts as well as mouse striatal and cortical neurons from an HdhQ111 mutant mouse. Here, we show that NCT-504 has a broader potential, and in addition reduces levels of Tau, a protein associated with Alzheimer’s disease, as well as other tauopathies. We …

Hpv-Related Oropharyngeal Cancer Early Detection In Gay And Bisexual Men Is An “Orphan” Practice: A Qualitative Analysis Among Healthcare Providers, I Niles Zoschke, Sarah L Bennis, J Michael Wilkerson, Cynthia L Stull, Alan G Nyitray, Samir S Khariwala, C Mark Nichols, B R Simon Rosser, Charlene Flash, Michael W Ross

Faculty, Staff and Student Publications

Introduction: Among US men, oropharyngeal cancer (cancer of the back of the mouth and throat) is the 8th most common cancer. If detected early, human papillomavirus (HPV)-16-associated oropharyngeal cancer has a high 5-year survival rate. Risk factors such as high numbers of oral sex partners, disparities in smoking and drinking, and low rates of HPV vaccination may put gay and bisexual men at even higher risk for oropharyngeal cancer.

Methods: We recruited 21 healthcare providers in Minneapolis-St. Paul, Minnesota and Houston, Texas to participate in semi-structured interviews. Nurses, physician assistants, dental hygienists, and dentists were asked about their clinical experiences …

Identification Of Microbiological Factors Associated With Periodontal Health Disparities, Bing-Yan Wang, Aize Cao, Meng-Hsuan Ho, Derek Wilus, Sally Sheng, Hsiu-Wan Meng, Elissa Guerra, Jianming Hong, Hua Xie

Faculty, Staff and Student Publications

The present study aimed at identifying risk factors associated with periodontitis development and periodontal health disparities with emphasis on differential oral microbiota. The prevalence of periodontitis is recently rising dentate adults in the US, which presents a challenge to oral health and overall health. The risk of developing periodontitis is higher in African Americans (AAs), and Hispanic Americans (HAs) than in Caucasian Americans (CAs). To identify potentially microbiological determinations of periodontal health disparities, we examined the distribution of several potentially beneficial and pathogenic bacteria in the oral cavities of AA, CA, and HA study participants. Dental plaque samples from 340 …

Sphingolipids In Neurodegenerative Diseases, Xueyang Pan, Debdeep Dutta, Shenzhao Lu, Hugo J Bellen

Faculty, Staff and Students Publications

Neurodegenerative Diseases (NDDs) are a group of disorders that cause progressive deficits of neuronal function. Recent evidence argues that sphingolipid metabolism is affected in a surprisingly broad set of NDDs. These include some lysosomal storage diseases (LSDs), hereditary sensory and autonomous neuropathy (HSAN), hereditary spastic paraplegia (HSP), infantile neuroaxonal dystrophy (INAD), Friedreich’s ataxia (FRDA), as well as some forms of amyotrophic lateral sclerosis (ALS) and Parkinson’s disease (PD). Many of these diseases have been modeled in Drosophila melanogaster and are associated with elevated levels of ceramides. Similar changes have also been reported in vertebrate cells and mouse models. Here, we …

Mass Spectrometry Imaging As An Emerging Tool For Studying Metabolism In Human Brain Organoids, Gerarda Cappuccio, Saleh M Khalil, Sivan Osenberg, Feng Li, Mirjana Maletic-Savatic

Faculty, Staff and Students Publications

Human brain organoids are emerging models to study human brain development and pathology as they recapitulate the development and characteristics of major neural cell types, and enable manipulation through an in vitro system. Over the past decade, with the advent of spatial technologies, mass spectrometry imaging (MSI) has become a prominent tool for metabolic microscopy, providing label-free, non-targeted molecular and spatial distribution information of the metabolites within tissue, including lipids. This technology has never been used for studies of brain organoids and here, we set out to develop a standardized protocol for preparation and mass spectrometry imaging of human brain …

Cerebellar Dysfunction In Rodent Models With Dystonia, Tremor, And Ataxia, Meike E Van Der Heijden, Roy V Sillitoe

Duncan NRI Faculty and Staff Publications

Dystonia is a movement disorder characterized by involuntary co- or over-contractions of the muscles, which results in abnormal postures and movements. These symptoms arise from the pathophysiology of a brain-wide dystonia network. There is mounting evidence suggesting that the cerebellum is a central node in this network. For example, manipulations that target the cerebellum cause dystonic symptoms in mice, and cerebellar neuromodulation reduces these symptoms. Although numerous findings provide insight into dystonia pathophysiology, they also raise further questions. Namely, how does cerebellar pathophysiology cause the diverse motor abnormalities in dystonia, tremor, and ataxia? Here, we describe recent work in rodents …

Function And Dysfunction Of The Dystonia Network: An Exploration Of Neural Circuits That Underlie The Acquired And Isolated Dystonias, Jason S Gill, Megan X Nguyen, Mariam Hull, Meike E Van Der Heijden, Ken Nguyen, Sruthi P Thomas, Roy V Sillitoe

Duncan NRI Faculty and Staff Publications

Dystonia is a highly prevalent movement disorder that can manifest at any time across the lifespan. An increasing number of investigations have tied this disorder to dysfunction of a broad "dystonia network" encompassing the cerebellum, thalamus, basal ganglia, and cortex. However, pinpointing how dysfunction of the various anatomic components of the network produces the wide variety of dystonia presentations across etiologies remains a difficult problem. In this review, a discussion of functional network findings in non-mendelian etiologies of dystonia is undertaken. Initially acquired etiologies of dystonia and how lesion location leads to alterations in network function are explored, first through …

An Initial Experience Of Completion Hemispherotomy Via Magnetic Resonance-Guided Laser Interstitial Therapy, Vijay M Ravindra, Lucia Ruggieri, Nisha Gadgil, Angela P Addison, Ilana Patino, David D Gonda, Jason Chu, Laura Whitehead, Anne Anderson, Gloria Diaz-Medina, Kimberly Houck, Akshat Katyayan, Laura Masters, Audrey Nath, Michael Quach, James John Riviello, Elaine Seto, Krystal Elizabeth Sully, Latanya Agurs, Sonali Sen, Maureen Handoko, Rohini Coorg, Irfan Ali, Daniel Ikeda, Howard Weiner, Daniel J Curry

Duncan NRI Faculty and Staff Publications

Introduction: In carefully selected patients with medically refractory epilepsy, disconnective hemispherotomy can result in significant seizure freedom; however, incomplete disconnection can result in ongoing seizures and poses a significant challenge. Completion hemispherotomy provides an opportunity to finish the disconnection. We describe the use of magnetic resonance-guided laser interstitial thermal ablation (MRgLITT) for completion hemispherotomy.

Methods: Patients treated with completion hemispherotomy using MRgLITT at our institution were identified. Procedural and seizure outcomes were evaluated retrospectively.

Results: Five patients (3 males) underwent six MRgLITT procedures (one child treated twice) for completion hemispherotomy at a median age of 6 years (range 1.8-12.9). Two …

Investigating The Viral Nature Of Neuronal Capsid Genes, Michael Manuel Long

Master's Theses

Memory formation is an essential part of everyday life. Neuronal communication is thought to be a key driver behind memory formation. There are two heavily studied methods of neuronal communication: chemical (neurotransmitter-mediated) and electrical (physically attached). When issues arise environmentally or genetically, people can experience one of many devastating memory disorders i.e. Alzheimer’s disease and other Dementias. Due to their prevalence, the treatments for disorders that hinder memory formation are a high priority for pharmaceutical companies. Currently, many of the treatments available focus on neurotransmitters or neuronal activity, and scientists are far from a cure. However, there may be an …

Correlation Between Nutritional Adequacy, Fe Content, Body Fat Percentage, And Muscle Mass Percentage With Physical Fitness, Sonya Hayu Indraswari, Mohammad Zen Rahfiludin, Ali Rosidi

Jurnal Keolahragaan

This study aims to determine the correlation between nutritional adequacy, Fe content, body fat percentage, and muscle mass percentage with physical fitness in football athletes. Adequacy of nutrients (energy, protein, fat, protein and Fe) was obtained with a 2×24 hour recall. The Fe content value of Fe was measured using the cyanmethemoglobin method and sTfR was measured using the ELWASA method. The percentage of fat and muscle was obtained by using a Bioelectrical Impedance Analysis tool. Physical fitness was measured using the ACSPFT (Asian Committee on Standardization of Physical Fitness Test) test which includes long jumps, pull-ups, sit-ups, sprints or …

Analysis Of Body Type, Dietary Intake, And Cardiorespiratory Function In College Soccer Players, Desty Ervira Puspaningtyas, Yuni Afriani, Silvi Lailatul Mahfida, Arta Farmawati, Wara Kushartanti

Jurnal Keolahragaan

This study aims to analyze body type, dietary intake, and cardiorespiratory function of college soccer players. A total of 32 players (20 players from Yogyakarta State University and 12 players from Gadjah Mada University) were involved in this study. The study was carried out in April - June 2014. Body type, or somatotype, was calculated based on anthropometric measurements (weight, height, epicondylar humerus and epicondylar femur width, arm and calf circumference, and subcutaneous fat thickness). 24-hour food recall was used to measure the athlete's dietary intake. VO₂max was used to describe the athlete's cardiorespiratory function, and it was …

Influence Of Walking Football On Decreasing Cholesterol And Blood Glycemic Level, Siswantoyo Siswantoyo, Kukuh Hardopo Putro, Japhet Ndayisenga, Rajesh Kumar, Rozita Abdul Latif

Jurnal Keolahragaan

The aims of this research to determine the impact of walking football on cholesterol and glycemic management in order to allow people to live healthily. This research is an experimental study with quantitative approach. The research sampling was 12 subjects from different category: (1) International students, (2) Local students, and employed persons. Technique collecting data using playground of futsal to assess variables like glycemic and cholesterol. Data analysis using independent test sample. To evaluate the subjects' health condition, the research proceeded by pre-test first, for the second step a walking football was given during 12 weeks within 3 times per …

The Effect Of Physical Exercise On Vital Lung Capacity In Tapak Suci Athletes, Sukadiono Sukadiono, Siti Fatimatus Zahrah, Dede Nasrullah, Supatmi Supatmi, Vika Ramadhana Fitriyani

Jurnal Keolahragaan

Exercise can have a physiological effect on the body, including the work system of the heart and lungs. The lungs have an important role in human life, where there is an exchange of oxygen with carbon dioxide through the respiratory process. In terms of health, exercise needs to be done by every human being, because exercise can increase the work of the lung muscles, whereas if the lung muscles work well, the ability to accommodate oxygen or the vital capacity of the human lungs also increases. The purpose of this study was to determine the effect of physical exercise on …

Small Extracellular Vesicle-Mediated Itgb6 Sirna Delivery Downregulates The Αvβ6 Integrin And Inhibits Adhesion And Migration Of Recipient Prostate Cancer Cells, Shiv Ram Krishn, Vaughn Garcia, Nicole M Naranjo, Fabio Quaglia, Christopher D Shields, Maisha A Harris, Andrew V Kossenkov, Qin Liu, Eva Corey, Dario C Altieri, Lucia R Languino

Department of Cancer Biology Faculty Papers

The αVβ6 integrin, an epithelial-specific cell surface receptor absent in normal prostate and expressed during prostate cancer (PrCa) progression, is a therapeutic target in many cancers. Here, we report that transcript levels of ITGB6 (encoding the β6 integrin subunit) are significantly increased in metastatic castrate-resistant androgen receptor-negative prostate tumors compared to androgen receptor-positive prostate tumors. In addition, the αVβ6 integrin protein levels are significantly elevated in androgen receptor-negative PrCa patient derived xenografts (PDXs) compared to androgen receptor-positive PDXs. In vitro, the androgen receptor-negative PrCa cells express high levels of the αVβ6 integrin compared to androgen receptor-positive PrCa cells. Additionally, …

The Effect Of Low Impact Aerobic Exercise On Increasing Physical Fitness For The Elderly, Cerika Rismayanthi, Muhammad Ikhwan Zein, Rizki Mulyawan, Risti Nurfadhila, Rifky Riyandi Prasetyawan, Muhammad Sigit Antoni

Jurnal Keolahragaan

This study aimed to investigate the effect of low-impact aerobic exercise on increasing physical fitness for the elderly. This one group of pretest-posttest quasi-experimental research recruited elderly subjects from Sumber Waras Elderly Association in Yogyakarta, Indonesia. Twenty-four elderly subjects aged 60-70 years old participated in the 16 sessions of aerobic exercise intervention. All subjects completed pre-and post-intervention physical fitness tests comprising the aerobic/cardiovascular (CV) endurance (2 minutes step test), upper body strength (arm curl test), lower body strength (chair stand test), upper body flexibility (back scratch test), core and lower body flexibility (sit and reach test) and balance (8 foot …

Body Fat Levels And Its Negative Correlation To Apnea Duration, Kukuh Pambuka Putra, Rambu Lawu Nedi Kristanti Retno Triandhini, Aditya Wicaksana, Sanfia Tesabela Messakh

Jurnal Keolahragaan

Apnea ability is the main determining factor of freediving performance. Apnea relates to physiological factors that might be experienced by a free diver hence its ability is highly likely to correlate with various factors of human physiology. One of the suspected factors that affect apnea duration is body fat level. The fact that fat is distributed in almost all parts of the body, including respiratory organs and tracts, arises the suspicion of a possible correlation between fat accumulation and apnea ability. This research studies the correlation between body fat level and apnea duration. 30 males, aged 18-22 were the research …

A Conserved Oxalyl-Coenzyme A Decarboxylase In Oxalate Catabolism, Ninghui Cheng, Vincent Paris, Xiaolan Rao, Xiaoqiang Wang, Paul A Nakata

Children’s Nutrition Research Center Staff Publications

The ability to biosynthesize oxalic acid can provide beneficial functions to plants; however, uncontrolled or prolonged exposure to this strong organic acid results in multiple physiological problems. Such problems include a disruption of membrane integrity, mitochondrial function, metal chelation, and free radical formation. Recent work suggests that a CoA-dependent pathway of oxalate catabolism plays a critical role in regulating tissue oxalate concentrations in plants. Although this CoA-dependent pathway of oxalate catabolism is important, large gaps in our knowledge of the enzymes catalyzing each step remain. Evidence that an oxalyl-CoA decarboxylase (OXC) catalyzes the second step in this pathway, accelerating the …

Evaluation Of The Efficacy Of The Anti-Ulcer Oral Mucosal Protective Agent Radoralex® In The Prevention And Treatment Of Radiation-Induced Oral Mucosal Reactions Induced During Treatment Of Nasopharyngeal Carcinoma, Jun Yin, Jirui Xie, Jiawei Lin, Chengyin Weng, Shun Lu, Peng Xu, Shuo Zhang, Cheng Luo, Yecai Huang, Lu Li, Jinyi Lang, Mei Feng

Faculty, Staff and Student Publications

The aim of this study was to evaluate the efficacy and safety of antiulcer oral mucosal protectant-RADoralex® in the prevention and treatment of radiation-induced oral mucosal reactions elicited during intensity-modulated radiation therapy (IMRT). for locally advanced nasopharyngeal carcinoma (NPC). A total of 90 patients with locally advanced NPC who developed post-treatment grade 1 oral mucositis were selected for this study. They were randomly assigned to the experimental group (n = 44) treated by mouth rinsing with the RADoralex® during radiochemotherapy and the control group (n = 43) treated by mouth rinsing with sodium bicarbonate solution, and the patients’ oral mucosal …

Kdm6b Variants May Contribute To The Pathophysiology Of Human Cerebral Folate Deficiency, Xiao Han, Xuanye Cao, Robert M Cabrera, Paula Andrea Pimienta Ramirez, Cuilian Zhang, Vincent T Ramaekers, Richard H Finnell, Yunping Lei

Faculty, Staff and Students Publications

(1) Background: The genetic etiology of most patients with cerebral folate deficiency (CFD) remains poorly understood. KDM6B variants were reported to cause neurodevelopmental diseases; however, the association between KDM6B and CFD is unknown; (2) Methods: Exome sequencing (ES) was performed on 48 isolated CFD cases. The effect of KDM6B variants on KDM6B protein expression, Histone H3 lysine 27 epigenetic modification and FOLR1 expression were examined in vitro. For each patient, serum FOLR1 autoantibodies were measured; (3) Results: Six KDM6B variants were identified in five CFD patients, which accounts for 10% of our CFD cohort cases. Functional experiments indicated that these …

Human Papillomavirus Dose Reminder Preferences Among Parents From A Diverse Clinical Sample: A Qualitative Study, Jacqueline M Hirth, Kayla A Eboreime, Leslie E Cofie, Richard E Rupp, Abbey B Berenson

Faculty, Staff and Students Publications

Reminders are an important method for encouraging patients to return for follow-up visits, such as for successive doses of the human papillomavirus (HPV) vaccine. However, patients may have preferences for different types of reminders. This study examined which reminder methods parents of pediatric patients found most useful and their thoughts on how the reminders helped them to complete their children's HPV vaccine series. This qualitative study was conducted on a purposively sampled group of parents who participated in a multi-level intervention intended to improve uptake and completion of the HPV vaccine series. Parents who agreed to participate were interviewed by …

Effect Of Neoadjuvant Chemotherapy On Intraoperative Core Temperature In Patients With Breast Cancer: A Retrospective Cohort Study, Daniel D Kim, Sarah M Desnyder, Patrick M Dougherty, Juan P Cata

Faculty, Staff and Student Publications

BACKGROUND: Clinical evidence suggests that chemotherapeutic agents are associated with neuropathy and peripheral autonomic dysfunction. However, the possible effects of neoadjuvant chemotherapy on intraoperative temperature remain poorly characterised.

METHODS: We evaluated patients who underwent a mastectomy for breast cancer between April 2016 and July 2020. Propensity scores were used to match patients who received neoadjuvant chemotherapy with those who did not, and intraoperative core temperature patterns were analysed in the matched cohort. The independent associations between vasopressor use and heart rate during general anaesthesia in the matched cohort were also analysed.

RESULTS: Data from 1764 patients were analysed (882 patients …

Habitat Imaging Biomarkers For Diagnosis And Prognosis In Cancer Patients Infected With Covid-19, Muhammad Aminu, Divya Yadav, Lingzhi Hong, Elliana Young, Paul Edelkamp, Maliazurina Saad, Morteza Salehjahromi, Pingjun Chen, Sheeba J Sujit, Melissa M Chen, Bradley Sabloff, Gregory Gladish, Patricia M De Groot, Myrna C B Godoy, Tina Cascone, Natalie I Vokes, Jianjun Zhang, Kristy K Brock, Naval Daver, Scott E Woodman, Hussein A Tawbi, Ajay Sheshadri, J Jack Lee, David Jaffray, D Code Team, Carol C Wu, Caroline Chung, Jia Wu

Faculty, Staff and Student Publications

OBJECTIVES: Cancer patients have worse outcomes from the COVID-19 infection and greater need for ventilator support and elevated mortality rates than the general population. However, previous artificial intelligence (AI) studies focused on patients without cancer to develop diagnosis and severity prediction models. Little is known about how the AI models perform in cancer patients. In this study, we aim to develop a computational framework for COVID-19 diagnosis and severity prediction particularly in a cancer population and further compare it head-to-head to a general population.

METHODS: We have enrolled multi-center international cohorts with 531 CT scans from 502 general patients and …

Loss Of Ubiquitin-Specific Peptidase 18 Destabilizes 14-3-3Ζ Protein And Represses Lung Cancer Metastasis, Zibo Chen, Lin Zheng, Yulong Chen, Xiuxia Liu, Masanori Kawakami, Lisa Maria Mustachio, Jason Roszik, Katherine V Ferry-Galow, Ralph E Parchment, Xin Liu, Thorkell Andresson, Gerard Duncan, Jonathan M Kurie, Jaime Rodriguez-Canales, Xi Liu, Ethan Dmitrovsky

Faculty, Staff and Student Publications

Cancer metastasis is a major cause of cancer-related mortality. Strategies to reduce metastases are needed especially in lung cancer, the most common cause of cancer mortality. We previously reported increased ubiquitin-specific peptidase 18 (USP18) expression in lung and other cancers. Engineered reduction of USP18 expression repressed lung cancer growth and promoted apoptosis. This deubiquitinase (DUB) stabilized targeted proteins by removing the complex interferon-stimulated gene 15 (ISG15). This study explores if the loss of USP18 reduced lung cancer metastasis. USP18 knock-down in lung cancer cells was independently achieved using small hairpin RNAs (shRNAs) and small interfering RNAs (siRNAs). USP18 knock-down reduced …

Measurement Of Force And Intramuscular Pressure Changes Related To Thrust Spinal Manipulation In An In Vivo Animal Model, William R Reed, Carla R Lima, Michael A K Liebschner, Christopher P Hurt, Peng Li, Maruti R Gudavalli

Faculty, Staff and Students Publications

Simple Summary

Spinal manipulation is recommended by clinical guidelines to treat low back pain. However, our understanding of how spinal manipulation alleviates pain and knowledge of the role that viscoelastic tissue properties play in the dampening of applied spinal manipulation forces in vivo is limited. Superficial and deep intramuscular pressures and applied forces were measured using miniature pressure catheters and a force transducer during low back spinal manipulation using a clinically available spinal manipulation device (Activator V) or a feedback motor in a deeply anesthetized animal model. Viscoelastic properties of muscle and other soft tissues greatly diminished applied spinal manipulative …

Prognostic Model For Predicting Overall And Cancer-Specific Survival Among Patients With Superficial Spreading Melanoma: A Seer Based Study, Qiang Ji, Jun Tang, Shulian Li, Junjie Chen

Faculty, Staff and Student Publications

Skin malignant melanoma is one of the most aggressive skin tumors. Superficial spreading melanoma (SSM) is the most common histological type, which can originate from different body skin sites, and some patients can still accumulate regional lymph nodes and even have distant metastasis in some cases. This study used the relevant data from the monitoring, epidemiology and results database of the National Cancer Institute database to study the overall survival (OS) and cancer-specific survival (CSS) of SSM patients and established an SSM nomogram to evaluate the prognosis of patients. A total of 13,922 patients were collected from the monitoring, epidemiology …