Medical Sciences Commons^™

Swimming Training On Moderate Intensity Significantly Reduces Total Cholesterol And Bodyweight On Hypercholesterolemic Rat Model, Anindya Mar'atus Sholikhah, Mochamad Ridwan

Jurnal Keolahragaan

It is well established that regular exercise has many positive benefits on health. Exercise at the appropriate intensity and level has been shown to improve lipid profile and reduce the risk of cardiovascular disease. This study investigates the effect of swimming training on total cholesterol levels and body weight in male Wistar rats. Thirty rats weighing 150-200 grams were randomly divided into one control (C) and two treatment groups (ST=swimming training, SED=sedentary activity). Rats in control (C) group were fed a standard diet, while rats in both treatment groups were fed high-fat diet-induced hypercholesterolemia for three weeks. Rats in ST …

Aktivitas Olahraga Bulu Tangkis Dan Respon Perubahan Asam Urat Darah Usia Produktif, Angkit Kinasih, Ronaldo Lomi Djara, Ferry Fredy Karwur

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui ada tidaknya respon perubahan pada kadar asam urat akibat aktivitas olahraga bulu tangkis. Desain penelitian ini adalah quasi eksperimen. Sampel yang digunakan sebanyak delapan responden dengan kriteria tertentu. Perlakukan aktivitas fisik dilakukan dua kali pada hari yang berbeda dengan 4 kali pengukuran kadar asam urat. Metode penelitian yang digunakan adalah repeated measure analysis. Ketika subyek diukur berulang kali dengan jumlah yang sedikit per percobaan maka repeated measures analysis dapat digunakan. Hasil penelitian menunjukkan bahwa perlakukan olahraga bulu tangkis memberikan respon perubahan yang signifikan terhadap perubahan kadar asam urat dengan nilai probabilitas sebesar 0,038. Hasil ini …

Hubungan Antara Sindrom Metabolik Dengan Kebugaran Jasmani Pada Lansia, Prijo Sudibjo, Cerika Rismayanthi, Krisnanda Dwi Apriyanto

Jurnal Keolahragaan

Prevalensi sindrom metabolik pada lansia cukup tinggi. Sindrom metabolik dapat dicegah salah satunya dengan aktivitas fisik. Aktivitas fisik juga dapat meningkatkan kebugaran kardiorespirasi, kelenturan sendi, keseimbangan dan kekuatan otot. Penelitian ini bertujuan untuk mengetahui hubungan antara sindrom metabolik dengan kebugaran kardiorespirasi, fleksibilitas, kekuatan, dan keseimbangan pada lansia. Penelitian ini merupakan penelitian cross sectional dengan teknik consecutive sampling pada 118 lansia di Yogyakarta. Sindrom metabolik ditetapkan berdasarkan kriteria diagnosis dari Adult Treatment Panel. Instrumen yang digunakan untuk mengumpulkan data adalah tes jalan 6 menit (6-minute walking test), sit and reach, hand grip dynamometer, leg and back dynamometer dan berdiri …

The Benefits Of Astaxanthin To Improve Pain Relief In Patients With Painful Diabetic Neuropathy: An Open-Label, Randomized Controlled Trial, Rizaldy Taslim Pinzon, Mary Rose Angelina Budi Harsana

Pharmaceutical Sciences and Research

Treatment of diabetic neuropathy is still carried out by providing symptomatic therapy, which only improves ± 50% of the total symptoms felt by patients, but does not tackle the underlying causes of the disease. Astaxanthin is a potent antioxidant, anti-inflammatory, and anti-diabetic carotenoid that could be an additional treatment option. We aimed to measure the effectiveness of administering astaxanthin as an additional therapy to improve the impact of pain and discomfort experienced daily by diabetes mellitus patients with painful diabetic neuropathy. We conducted a randomized experimental study with an open label design of 36 patients who had been diagnosed with …

The Effect Of Heparinoid As Systemic Prophylactic Anticoagulants On Covid-19 Patient Mortality And Its Safety Profiles: A Systematic Review And Meta-Analysis, Ariska Deffy Anggarany, Rani Sauriasari, Muhammad Alkaff, Famila Takhwifa, Hayatun Nufus, Diana Paramita

Pharmaceutical Sciences and Research

Coagulopathy is one of the complications of COVID-19 and is associated with a higher risk of mortality. However, evidence regarding the effectiveness and safety of anticoagulant therapy in various doses among COVID-19 patients is limited. This systematic review and meta-analysis aims to review and explore the effect of using heparinoids as a systemic anticoagulant at prophylactic doses on mortality in COVID-19 patients. Systematic searches were conducted of various databases (Pubmed, ScienceDirect, SpringerLink, Scopus, and ProQuest) covering the period 2019-2021. We assessed the quality of the articles using the STROBE checklist. Studies with a high risk of bias were excluded before …

An Assessment Of The Diagnostic Value In Syncope Workup: A Retrospective Study, Ashan T. Hatharasinghe, Kayvon Etebar, Ryan Wolsky, Hossein Akhondi, Napatkamon Ayutyanont

HCA Healthcare Journal of Medicine

Background

Patients with syncope often undergo costly testing, despite current guidelines and data supporting the contrary.

Objective

To determine the diagnostic value through positivity rate of electrocardiogram (EKG), computed tomography (CT) of the brain, magnetic resonance imaging (MRI) of the brain, transthoracic echocardiogram, nuclear and pharmacologic cardiac stress test, tilt table test and carotid ultrasound in patients diagnosed with syncope.

Methods

This is a retrospective study of 10,036 adults presenting to the emergency department or hospitalized with a primary diagnosis of syncope at 8 acute care facilities in the southwest United States from January 1, 2019, to December 31, 2019. …

Steroid-Refractory Lichenoid Eruption Associated With Pembrolizumab In A Patient With Non-Small Cell Lung Cancer, Leon Kou, Sanjay Agarwal, Alyssa Miceli, Logan Kolb, Karthik Krishnamurthy, Shawn Schmieder

HCA Healthcare Journal of Medicine

Programmed cell death receptor 1 (PD-1) inhibitors are promising and effective treatments for various cancers. Cutaneous adverse events, such as lichenoid drug eruptions, are well-known common side effects associated with PD-1 inhibitors. Lichenoid drug eruptions associated with PD-1 inhibitors show rapid improvement with high potency topical steroids and do not require cessation of the offending drug. We present the case of an 84-year-old female with progressive pembrolizumab therapy-associated lichenoid eruption that was resistant to several treatments and ultimately required discontinuation of pembrolizumab and treatment with methotrexate to resolve. This report includes histological findings of the pembrolizumab-associated lichenoid eruption.

Characterization Of A New Whim Syndrome Mutant Reveals Mechanistic Differences In Regulation Of The Chemokine Receptor Cxcr4, Jiansong Luo, Francesco De Pascali, G Wendell Richmond, Amer M Khojah, Jeffrey L Benovic

Department of Biochemistry and Molecular Biology Faculty Papers

WHIM syndrome is a rare immunodeficiency disorder that is characterized by warts, hypogammaglobulinemia, infections, and myelokathexis. While several gain-of-function mutations that lead to C-terminal truncations, frame shifts and point mutations in the chemokine receptor CXCR4 have been identified in WHIM syndrome patients, the functional effect of these mutations are not fully understood. Here, we report on a new WHIM syndrome mutation that results in a frame shift within the codon for Ser339 (S339fs5) and compare the properties of S339fs5 with wild-type CXCR4 and a previously identified WHIM syndrome mutant, R334X. The S339fs5 and R334X mutants exhibited significantly increased signaling compared …

Gambaran Epidemiologi Hepatitis A Berdasarkan Data Kejadian Luar Biasa Dan Data Kasus Di Kabupaten Bogor Tahun 2019, Nurjannah Nurjannah, Asri Adisasmita Prof

Jurnal Epidemiologi Kesehatan Indonesia

Kejadian luar biasa (KLB) Hepatitis A Kabupaten Bogor terus meningkat setiap tahunnya. Pada tahun 2018 terdapat 29 kasusdan pada tahun 2019 meningkat hingga 495 kasus. Penelitian ini bertujuan untuk menggambarkan epidemiologi berdasarkan orang, tempat, waktu. Hasil penelitian ini diharapkan dapat digunakan sebagai dasar upaya intevensi yang lebih optimaldan tepat sasaran. Data penelitian diambil dari laporan KLB dandari data kasus Hepatitis A di Dinas Kesehatan Kabupaten Bogor dari Januari-Desember 2019 dan didokumentasikan ke dalam tabel observasi, pengambilan data dilakukan pada Bulan Februari2020 dengan desain penelitian case series. Hasil penelitian ini menunjukkan dari 495 kasus yang mengalami gejala klinis,11,3% terkonfimasi positif, sebagian …

Single-Cell Analysis Of Aneurysmal Aortic Tissue In Patients With Marfan Syndrome Reveals Dysfunctional Tgf-Β Signaling, Ashley Dawson, Yanming Li, Yang Li, Pingping Ren, Hernan G. Vasquez, Chen Zhang, Kimberly R. Rebello, Waleed Ageedi, Alon R. Azares, Aladdein Burchett Mattar, Mary Burchett Sheppard, Hong S. Lu, Joseph S. Coselli, Lisa A. Cassis, Alan Daugherty, Ying H. Shen, Scott A. Lemaire

Saha Cardiovascular Research Center Faculty Publications

The molecular and cellular processes leading to aortic aneurysm development in Marfan syndrome (MFS) remain poorly understood. In this study, we examined the changes of aortic cell populations and gene expression in MFS by performing single-cell RNA sequencing (scRNA seq) on ascending aortic aneurysm tissues from patients with MFS (n = 3) and age-matched non-aneurysmal control tissues from cardiac donors and recipients (n = 4). The expression of key molecules was confirmed by immunostaining. We detected diverse populations of smooth muscle cells (SMCs), fibroblasts, and endothelial cells (ECs) in the aortic wall. Aortic tissues from MFS showed alterations …

Does Quran Memorization Influence Adolescents’ Intelligence Quotient And Memory Level?: A Cross-Sectional Study In Malaysia, Ismarulyusda Ishak, Amira Aqilah Shaidin, Dzalani Harun, Nor Malia Abdul Warif, Vanitha Mariappan, Arimi Fitri Mat Ludin, Ahmad Rohi Ghazali, Farah Wahida Ibrahim, Normah Che Din

Makara Journal of Health Research

Background: The process of memorizing various reading materials enhances the brain capacity to process, store, and assemble memories and increases the intelligence quotient (IQ) level. However, the connection between the memorization of Quran and the enhancement of memory level is not fully understood. Hence, a cross-sectional study was conducted to examine the correlation between Quran memorization and the IQ level among Muslim adolescents from selected Islamic schools in Selangor, Malaysia.

Methods: The Wechsler Abbreviated Scale of Intelligence was used to measure the student’s IQ level, whereas Digit Span and Rey Auditory Verbal Learning Test were used to evaluate …

Hubungan Antara Riwayat Kunjungan Antenatal Care (Anc) Dengan Kejadian Lahir Mati Di Indonesia, Lila Kesuma Hairani, Asri Adisasmita Prof

Jurnal Epidemiologi Kesehatan Indonesia

Pada tahun 2015, sebanyak 65% dari total kelahiran mati diseluruh dunia disumbang oleh sepuluh negara dengan jumlah kelahiran mati terbanyak. Indonesia menempati posisi ke-8 dengan total sebanyak 73.400 kejadian dengan angka lahir mati (stillbirth rate/SBR) sebesar 13 per 1.000 kelahiran. Rendahnya cakupan layanan antenatal diduga berhubungan secara kuatdengan rendahnya lahir mati antepartum. Studi ini dilakukan dengan tujuan untuk melihat hubungan riwayat kunjungan antenatal (antenatal care/ANC) dengan kejadian lahir mati diIndonesia menggunakan data sekunder yang bersumber dari IFLS 5 tahun 2014. Desain penelitian ini berupa studi potonglintang dengan total sampel yang digunakan adalah sebesar 6.314 kelahiran. Analisis data dilakukan hingga tingkat …

Hubungan Faktor Pejamu Dan Lingkungan Dengan Pneumonia Balita Di 5 Provinsi Di Pulau Sulawesi, Alfi Lailiyah, Nuning Maria Kiptiyah

Jurnal Epidemiologi Kesehatan Indonesia

Pneumonia merupakan penyakit menular yang menginfeksi paru-paru. Period prevalence pneumonia balita di 5 provinsi di Sulawesi lebih tinggi dibanding angka nasional. Penelitian ini bertujuan untuk mendapatkan gambaran dan faktor-faktor yang berhubungan dengan pneumonia balita di 5 Provinsi di Pulau Sulawesi. Data penelitian ini bersumber dari Survei Demografi dan Kesehatan Indonesia (SDKI) 2012. Desain dalam penelitian ini adalah cross-sectional dengan uji chi-square dan hasil ukur prevalence ratio (PR). Variabel pada penelitian ini adalah faktor pejamu (umur balita, jenis kelamin, berat badan lahir, pemberian vitamin A, status imunisasi campak dan DPT) dan faktor lingkungan (pendidikan ibu, tempat tinggal, bahan bakar memasak, keberadaan …

Hubungan Hipertensi Dengan Mortalitas Pasien Covid-19 Di Tangerang Selatan, Choirunnisa Choirunnisa, Helda Helda

Jurnal Epidemiologi Kesehatan Indonesia

Covid-19 (Coronavirus Disease-2019) adalah penyakit yang disebabkan oleh virus SARS-CoV-2 (Severe Acute Respiratory Syndrome-Coronavirus-2). Usia tua (>65 tahun), jenis kelamin laki-laki, hipertensi, penyakit kardiovaskular, diabetes, PPOK dan kanker dapat meningkatkan risiko kematian akibat Covid-19. Kota Tangerang Selatan berpotensi mengalami kenaikan kasus Covid-19 karena berbatasan langsung dengan DKI Jakarta yang merupakan episentrum persebaran Covid-19, sehingga peneliti tertarik untuk melakukan penelitian ini. Desain penelitian ini adalah studi analitik kasus kontrol menggunakan data sekunder dari penelusuran epidemiologi (PE) Dinas Kesehatan Kota Tangerang Selatan bulan Maret 2020-Juli 2021. Data PE diperoleh dari portal New All Record (NAR) yang memuat data pasien Covid-19 termasuk …

Hubungan Status Gizi Dengan Prestasi Belajar Siswa Kelas 1-3 Sekolah Dasar, Muhammad Hidayat Sahid, Asri Adisasmita Prof, Ratna Djuwita

Jurnal Epidemiologi Kesehatan Indonesia

Status gizi merupakan ukuran keberhasilan dalam pemenuhan nutrisi anak yang diukur berdasarkan berat badan dan tinggi badan anak. Data status gizi pada anak usia umur 5-12 tahun di DKI Jakarta menunjukkan underweight 14,0%, stunting 22,7%, wasting 9,9%, dan overweight 6,8%. Data secara spesifik untuk wilayah Jakarta Selatan adalah underweight 7,4%, stunting 17,8%, wasting 6,3%, dan overweight 7,3%. Dari data tersebut didapatkan gambaran mengenai permasalahan gizi yang terjadi di DKI Jakarta. Permasalahan gizi memiliki dampak pada tumbuh kembang anak. Gizi merupakan salah satu faktor penting yang berpengaruh terhadap prestasi akademik siswa. Tujuan dari penelitian ini adalah untuk mengetahui hubungan status gizi …

A Snapshot Investigation Into The Availability And Access To Codeine-Containing Medications Amongst The Irish Population., Katie O'Brien, Pádraig Mckeown, Joseph Phelan

International Undergraduate Journal of Health Sciences

Codeine phosphate is a mild to moderate analgesic and has a weak cough suppressant activity that is available to purchase as an over the counter (OTC) painkiller in Ireland as a compound product. In recent years, countries such as Australia have introduced laws that prohibit the sale of the drug without a valid prescription. This move by Australia begs the question as to why this legislation was introduced and should Ireland follow suit.

The primary aims of the study were to determine knowledge among the general public of codeine-containing medications and their usage levels. The survey also investigated whether current …

An Investigation Into The Prevalence Of Vancomycin-Resistant Enterococci Within An Irish Hospital, Dylan Casey

International Undergraduate Journal of Health Sciences

Vancomycin-resistant enterococci (VRE) are well-recognised nosocomial pathogens that pose a significant threat to public health. Associated with poorer clinical outcomes than their vancomycin-sensitive counterparts, the prevalence of VRE in Ireland has increased in recent times, with the European Antimicrobial Resistance Surveillance Network reporting that out of 29 countries, Ireland demonstrated the highest rates of vancomycin resistance among invasive Enterococcus faecium isolates between 2011 and 2014 (2011; 34.9%, 2012; 44.0%, 2013; 42.7%, 2014; 45.1%). Herein, we investigate VRE prevalence in the Mercy University Hospital (MUH) – an acute care hospital in southern Ireland. A total of 21 first-time VRE infections were …

Inhibition Of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade And Promotes Recovery After Spinal Cord Injury, Chen Guang Yu, Vimala Bondada, Hina Iqbal, Kate L. Moore, John C. Gensel, Subbarao Bondada, James W. Geddes

Physiology Faculty Publications

Microglia/astrocyte and B cell neuroimmune responses are major contributors to the neurological deficits after traumatic spinal cord injury (SCI). Bruton tyrosine kinase (BTK) activation mechanistically links these neuroimmune mechanisms. Our objective is to use Ibrutinib, an FDA-approved BTK inhibitor, to inhibit the neuroimmune cascade thereby improving locomotor recovery after SCI. Rat models of contusive SCI, Western blot, immunofluorescence staining imaging, flow cytometry analysis, histological staining, and behavioral assessment were used to evaluate BTK activity, neuroimmune cascades, and functional outcomes. Both BTK expression and phosphorylation were increased at the lesion site at 2, 7, 14, and 28 days after SCI. Ibrutinib …

Spontaneous Coronary Artery Dissection, Amy N. Wagner

Graduate Dissertations and Theses

Spontaneous coronary artery dissection (SCAD) causes 35% of myocardial infarctions in women under the age of 50 years old. Typically, these are healthy, active women without risk factors for atherosclerotic coronary artery syndromes. SCAD is a myocardial infarction caused by a coronary arterial dissection and not atherosclerotic plaques. Because of this, those effected do not present with the typical risk factors associated with acute atherosclerotic coronary syndromes. Therefore, clinicians must maintain a high level of suspicion for SCAD in young, healthy, women presenting with chest pain. Observation and pharmacological therapies are the preferred methods of treatment for a SCAD event. …

Treatment-Specific Hippocampal Subfield Volume Changes With Antidepressant Medication Or Cognitive-Behavior Therapy In Treatment-Naive Depression., Hua-Hsin Tai, Jungho Cha, Faezeh Vedaei, Boadie W Dunlop, W Edward Craighead, Helen S Mayberg, Ki Sueng Choi

Department of Neuroscience Faculty Papers

Background: Hippocampal atrophy has been consistently reported in major depressive disorder with more recent focus on subfields. However, literature on hippocampal volume changes after antidepressant treatment has been limited. The first-line treatments for depression include antidepressant medication (ADM) or cognitive-behavior therapy (CBT). To understand the differential effects of CBT and ADM on the hippocampus, we investigated the volume alterations of hippocampal subfields with treatment, outcome, and chronicity in treatment-naïve depression patients. Methods: Treatment-naïve depressed patients from the PReDICT study were included in this analysis. A total of 172 patients who completed 12 weeks of randomized treatment with CBT (n …

Levels Of Angiotensin-Converting Enzyme And Apolipoproteins Are Associated With Alzheimer’S Disease And Cardiovascular Diseases, Chun Xu, Debra Garcia, Yongke Lu, Kaysie Ozuna, Donald A. Adjeroh, Kesheng Wang

Health & Biomedical Sciences Faculty Publications

Angiotensin-converting enzyme-1 (ACE1) and apolipoproteins (APOs) may play important roles in the development of Alzheimer’s disease (AD) and cardiovascular diseases (CVDs). This study aimed to examine the associations of AD, CVD, and endocrine-metabolic diseases (EMDs) with the levels of ACE1 and 9 APO proteins (ApoAI, ApoAII, ApoAIV, ApoB, ApoCI, ApoCIII, ApoD, ApoE, and ApoH). Non-Hispanic white individuals including 109 patients with AD, 356 mild cognitive impairment (MCI), 373 CVD, 198 EMD and controls were selected from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) dataset. Multivariable general linear model (GLM) was used to examine the associations. ApoE ε4 allele was associated with …

Platelet Micrornas Inhibit Primary Tumor Growth Via Broad Modulation Of Tumor Cell Mrna Expression In Ectopic Pancreatic Cancer In Mice, Jeremy G.T. Wurtzel, Sophia Lazar, Sonali Sikder, Kathy Q Cai, Igor Astsaturov, Andrew S Weyrich, Jesse W Rowley, Lawrence E. Goldfinger

Department of Medicine Faculty Papers

We investigated the contributions of platelet microRNAs (miRNAs) to the rate of growth and regulation of gene expression in primary ectopic tumors using mouse models. We previously identified an inhibitory role for platelets in solid tumor growth, mediated by tumor infiltration of platelet microvesicles (microparticles) which are enriched in platelet-derived miRNAs. To investigate the specific roles of platelet miRNAs in tumor growth models, we implanted pancreatic ductal adenocarcinoma cells as a bolus into mice with megakaryocyte-/platelet-specific depletion of mature miRNAs. We observed an ~50% increase in the rate of growth of ectopic primary tumors in these mice compared to controls …

Recombinant Human Proteoglycan 4 Regulates Phagocytic Activation Of Monocytes And Reduces Il-1Β Secretion By Urate Crystal Stimulated Gout Pbmcs, Sandy Elsayed, Gregory D. Jay, Ralph Cabezas, Marwa Qadri, Tannin A. Schmidt, Khaled A. Elsaid

Pharmacy Faculty Articles and Research

Objectives

To compare phagocytic activities of monocytes in peripheral blood mononuclear cells (PBMCs) from acute gout patients and normal subjects, examine monosodium urate monohydrate (MSU) crystal-induced IL-1β secretion ± recombinant human proteoglycan 4 (rhPRG4) or interleukin-1 receptor antagonist (IL-1RA), and study the anti-inflammatory mechanism of rhPRG4 in MSU stimulated monocytes.

Methods

Acute gout PBMCs were collected from patients in the Emergency Department and normal PBMCs were obtained from a commercial source. Monocytes in PBMCs were identified by flow cytometry. PBMCs were primed with Pam3CSK4 (1μg/mL) for 24h and phagocytic activation of monocytes was determined using fluorescently labeled latex beads. MSU …

Dysregulation Of Dna Methylation And Epigenetic Clocks In Prostate Cancer Among Puerto Rican Men, Anders Berglund, Jaime Matta, Jarline Encarnación-Medina, Carmen Ortiz-Sanchéz, Julie Dutil, Raymond Linares, Joshua Marcial, Caren Abreu-Takemura, Natasha Moreno, Ryan Putney, Ratna Chakrabarti, Hui Yi Lin, Kosj Yamoah, Carlos Diaz Osterman, Liang Wang, Jasreman Dhillon, Youngchul Kim, Seung Joon Kim, Gilberto Ruiz-Deya, Jong Y. Park

School of Public Health Faculty Publications

In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (n = 17) and indolent (n = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. …

Adipose-Specific Pparα Knockout Mice Have Increased Lipogenesis By Pask–Srebp1 Signaling And A Polarity Shift To Inflammatory Macrophages In White Adipose Tissue, Terry D. Hinds, Jr., Zachary A. Kipp, Mei Xu, Frederique B. Yiannikouris, Andrew J. Morris, Donald F. Stec, Walter Wahli, David E. Stec

Pharmacology and Nutritional Sciences Faculty Publications

The nuclear receptor PPARα is associated with reducing adiposity, especially in the liver, where it transactivates genes for β-oxidation. Contrarily, the function of PPARα in extrahepatic tissues is less known. Therefore, we established the first adipose-specific PPARα knockout (Ppara^FatKO) mice to determine the signaling position of PPARα in adipose tissue expansion that occurs during the development of obesity. To assess the function of PPARα in adiposity, female and male mice were placed on a high-fat diet (HFD) or normal chow for 30 weeks. Only the male Ppara^FatKO animals had significantly more adiposity in the inguinal white …

Recurrent High-Impact Mutations At Cognate Structural Positions In Class A G Protein-Coupled Receptors Expressed In Tumors, Eunna Huh, Jonathan Gallion, Melina A Agosto, Sara J Wright, Theodore G Wensel, Olivier Lichtarge

Faculty, Staff and Students Publications

G protein-coupled receptors (GPCRs) are the largest family of human proteins. They have a common structure and, signaling through a much smaller set of G proteins, arrestins, and effectors, activate downstream pathways that often modulate hallmark mechanisms of cancer. Because there are many more GPCRs than effectors, mutations in different receptors could perturb signaling similarly so as to favor a tumor. We hypothesized that somatic mutations in tumor samples may not be enriched within a single gene but rather that cognate mutations with similar effects on GPCR function are distributed across many receptors. To test this possibility, we systematically aggregated …

Putative Concussion Biomarkers Identified In Adolescent Male Athletes Using Targeted Plasma Proteomics, Michael R. Miller, Michael Robinson, Lisa Fischer, Alicia Dibattista, Maitray A. Patel, Mark Daley, Robert Bartha, Gregory A. Dekaban, Ravi S. Menon, J. Kevin Shoemaker, Eleftherios P. Diamandis, Ioannis Prassas, Douglas D. Fraser

Medical Biophysics Publications

Sport concussions can be difficult to diagnose and if missed, they can expose athletes to greater injury risk and long-lasting neurological disabilities. Discovery of objective biomarkers to aid concussion diagnosis is critical to protecting athlete brain health. To this end, we performed targeted proteomics on plasma obtained from adolescent athletes suffering a sports concussion. A total of 11 concussed male athletes were enrolled at our academic Sport Medicine Concussion Clinic, as well as 24 sex-, age- and activity-matched healthy control subjects. Clinical evaluation was performed and blood was drawn within 72 h of injury. Proximity extension assays were performed for …

Eliminating Contamination In Umbilical Cord Blood Culture Sampling For Early-Onset Neonatal Sepsis., Vilmaris Quinones Cardona, Vanessa Lowery, David Cooperberg, Endla K Anday, Alison J Carey

Department of Pediatrics Faculty Papers

Introduction: Despite the advantages of umbilical cord blood culture (UCBC) use for diagnosis of early onset sepsis (EOS), contamination rates have deterred neonatologists from its widespread use. We aimed to implement UCBC collection in a level III neonatal intensive care unit (NICU) and apply quality improvement (QI) methods to reduce contamination in the diagnosis of early onset sepsis. Methods: Single-center implementation study utilizing quality improvement methodology to achieve 0% contamination rate in UCBC samples using the Plan-Do-Study-Act (PDSA) model for improvement. UCBC was obtained in conjunction with peripheral blood cultures (PBC) in neonates admitted to the NICU due to maternal …

Effects Of Subthalamic Deep Brain Stimulation On Treating Motor Symptoms In Early Parkinson's Disease, Emily Reale, Gina Legaluppi

Physician Assistant Capstones, 2020-current

Objective: Assess the efficacy of subthalamic nucleus deep brain stimulation (STN DBS) versus solely optimal pharmacological use in early Parkinson's Disease to improve motor dysfunction. Design: Systematic Literature Review. Methods: Searches were completed on PubMed utilizing the MeSH terms: “subthalamic, deep brain stimulation, and early parkinson.” Using PubMed the following limits and terms were used: published in the last 10 years, randomized controlled trial, English, human, and removing duplicates. A total of three studies resulted after the search. Results: All three studies showed statistically significant results in reduction in disease progressive motor symptoms. Additionally, at the end of each study …

The Mrna-Lnp Platform's Lipid Nanoparticle Component Used In Preclinical Vaccine Studies Is Highly Inflammatory, Sonia Ndeupen, Zhen Qin, Sonya Jacobsen, Aurélie Bouteau, Henri Estanbouli, Botond Z. Igyártó

Department of Microbiology and Immunology Faculty Papers

Vaccines based on mRNA-containing lipid nanoparticles (LNPs) are a promising new platform used by two leading vaccines against COVID-19. Clinical trials and ongoing vaccinations present with varying degrees of protection levels and side effects. However, the drivers of the reported side effects remain poorly defined. Here we present evidence that Acuitas' LNPs used in preclinical nucleoside-modified mRNA vaccine studies are highly inflammatory in mice. Intradermal and intramuscular injection of these LNPs led to rapid and robust inflammatory responses, characterized by massive neutrophil infiltration, activation of diverse inflammatory pathways, and production of various inflammatory cytokines and chemokines. The same dose of …