Medicine and Health Sciences Commons^™

In Vitro And In Vivo Effects Of Concentrated Growth Factor On Cells And Tissues, Fahimeh Tabatabaei, Zahra Aghamohammadi, Lobat Tayebi

School of Dentistry Faculty Research and Publications

This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty-five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, …

Extra Nodal Rosai-Dorfman Disease Originating In The Nasal And Paranasal Complex And Gnathic Bones: A Systematic Analysis Of Seven Cases And Review Of Literature, Junu Ojha, Yeshwant B. Rawal, Jason L. Hornick, Kelly Magliocca, David R. Montgomery, Robert D. Foss, Kevin R. Torske, Brent Accurso

School of Dentistry Faculty Research and Publications

Rosai-Dorfman disease (RDD) is a benign, self-limiting histiocytosis of unknown etiology. The classic form of the condition includes a painless cervical lymphaenopathy [sic] accompanied by fever, weight loss and an elevated ESR. Extra nodal RDD (ENRDD) is most frequent in the head and neck. Thirty-eight cases of ENRDD have been described. Seven cases of ENRDD were identified in our pathology biopsy services. The demographic and clinical information was tabulated logically on the basis of age, gender, location and presence or absence of symptoms, treatment and follow-up. Radiographic and histopathological features were also examined. The findings in these cases were correlated …

Three-Dimensional Bio-Printing And Bone Tissue Engineering: Technical Innovations And Potential Applications In Maxillofacial Reconstructive Surgery, Muhja Salah, Lobat Tayebi, Keyvan Moharamzadeh, Farhad B. Naini

School of Dentistry Faculty Research and Publications

Background

Bone grafting has been considered the gold standard for hard tissue reconstructive surgery and is widely used for large mandibular defect reconstruction. However, the midface encompasses delicate structures that are surrounded by a complex bone architecture, which makes bone grafting using traditional methods very challenging. Three-dimensional (3D) bioprinting is a developing technology that is derived from the evolution of additive manufacturing. It enables precise development of a scaffold from different available biomaterials that mimic the shape, size, and dimension of a defect without relying only on the surgeon’s skills and capabilities, and subsequently, may enhance surgical outcomes and, in …

Role Of Iron On Physical And Mechanical Properties Of Brushite Cements, And Interaction With Human Dental Pulp Stem Cells, Sahar Vahabzadeh, Sarah Fleck, Joshua Marble, Fahimeh Tabatabaei, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Improving the physical, mechanical and biological properties of brushite cements (BrC) is of a great interest for using them in bone and dental tissue engineering applications. The objective of this study was to incorporate iron (Fe) at different concentrations (0.25, 0.50, and 1.00 wt%) to BrC and study the role of Fe on phase composition, setting time, compressive strength, and interaction with human dental pulp stem cells (hDPSCs). Results showed that increase in Fe concentration increases the β-tricalcium phosphate (β-TCP)/dicalcium phosphate dihydrate (DCPD) ratio and prolongs the initial and final setting time due to effective role of Fe on stabilizing …

Effect Of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium On Cerulein-Induced Acute Pancreatitis In Mice, Kamal Abdolmohammadi, Tayebeh Mahmoudi, Shahrzad Nojehdehi, Lobat Tayebi, Seyed Mahmoud Hashemi, Farshid Noorbakhsh, Alireza Abdollahi, Masoud Soleimani, Behrouz Nikbin, Mohammad Hossein Nicknam

School of Dentistry Faculty Research and Publications

Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice.

Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μg/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 µL/mice/30 min × 3 times). …

Does The Method Of Visualization Impact The Performance Of A New Surgical Task In Novice Subjects?, Mikaeel Kassam, Srikant Chakravarthi, Austin Epping, Sarah Erpenbeck, Maharaj Singh, Melanie B. Fukui, Amin Kassam, Richard Rovin

School of Dentistry Faculty Research and Publications

Introduction

Evolution of optical technology from two-dimensional to three-dimensional (3D) systems has come with an associated loss of stereoscopy and 3D depth perception. This report compares performance of surgical tasks in unbiased subjects using these systems.

Methods

Untrained subjects were randomized into two groups, robotically operated video optical telescopic-microscope (ROVOT) or surgical microscope (microscope). Subjects sutured and tied knots. Completion time, NASA-Task Load Index (TLX), and galvanic skin responses were analyzed.

Results

Intergroup analysis of suture completion time indicated that microscope use was significantly faster compared to ROVOT, whether used first or second. Regardless of which methodology was used first, …

Isolation And Identification Of Extended Spectrum Β-Lactamases (Esbls) Escherichia Colifrom Minced Camel Meat In Eastern Province, Saudi Arabia, Waleed Rizk El-Ghareeb, Sherief M. Abdel-Raheem, Theeb M. Al-Marri, Fanan A. Alaql, Mahmoud M. Fayez

The Thai Journal of Veterinary Medicine

Antimicrobial resistance is an increasingly serious threat to global public health that requires action across all government sectors and society. The aim of this study was to determine the rate of extended-spectrum β-lactamases (ESBL)-producing E. coliisolation from minced camel meat and identify the phenotype and genotype of the ESBL. A total of 150 samples were collected randomly from butchers’ shops in Al-Ahsa, Saudi Arabia. The results indicated that, overall, 17 (11.3 %) E. coliisolates were recovered from the minced meat samples. The isolates were classified biochemically at the species level using the VITEK 2 system. The antibiotic susceptibility of E. …

Prevalence Of Bacterial Contamination In Uncooked Sticky Rice Samples From Markets In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita

The Thai Journal of Veterinary Medicine

We investigated the prevalence of bacterial contamination in uncooked sticky rice samples obtained from markets in Japan. Between June and August, 2019, a total of 25 sticky rice samples were purchased from shops in the Tokyo area. Twenty-two (88.0%) and 10 (40.0%) of 25 sticky rice samples harbored 3.06±0.54 log spc/g of standard plates counts and 2.17±0.23 log cfu/g of enterobacteriaceae bacteria counts, respectively. Three species of Enterobacteriaceae, Pantoea dispersa, P. ananati, and Kosakonia cowanii, were identified by MALDI-TOFMS-based test. Three (12.0%) of 25 sticky rice samples harbored 2.00 log cfu/g of Bacillusspecies. Bacillusspp. was not isolated after heat treatment …

A Study Of Factors Associated With Arterial Thromboembolism In Cats Affected With Hypertrophic Cardiomyopathy In Thailand, Natthakritta Nokthong, Pornsawan Pongsawat, Phattareeporn Leurdthainarong, Sermsakul Sritrirasri, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Feline arterial thromboembolism (ATE) is an acute condition that is usually associated with cardiomyopathy in cats. To date, there has been no report of factors associated with ATE in cats affected with hypertrophic cardiomyopathy (HCM) in Thailand. The purpose of this study was to find factors related to ATE in cats with HCM. A retrospective study was performed in 70 cats with HCM dividing them into two groups (26 ATE and 44 non-ATE groups) to analyze factors relating to ATE including sex, breed, age, weight, heart sounds, radiographic findings, echocardiographic findings and the environmental temperature. The result of univariable logistic …

Antimicrobial Activity Of Formulated Clove Essential Oil Spray Against Biofilm-Forming Malassezia Pachydermatisand Staphylococcus Pseudintermediusclinical Isolates, Jareerat Aiemsaard, Chuchat Kamollerd, Pimchanok Suwannathada, Eakachai Thongkham

The Thai Journal of Veterinary Medicine

Clove essential oil, Formulation preparation, Antimicrobial activity, Malassezia pachydermatis, Staphylococcus pseudintermedius

Efficiency Of Heat Reduction In Barns During Rainy Season And Barn Characteristics: Their Relationship To Reproductive Performance Of Dairy Cows In A Tropical Environment, Watcharapong Choonkham, Thuwanon Boonkerd, Siriporn Kanwichai, Peter Vos, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objectives of this study were toevaluate the impact of efficiency of heat reduction on barns during the rainy season and barn characteristics on the reproductive performance of dairy cows in a tropical environment. Data of cows from 125 small-holder dairy farms in Chiang Mai, Thailand, during May to August 2014 was used. Reproductive performance indices, e.g. days to first insemination (DI) and days open (DO), were used as dependent variables. Microclimate factors included differences in temperature (TEMP_DIFF) and relative humidity (RH_DIFF) between the inside and outside of barns. From the total of 5,255 lactation data, 27 and 1,398 data …

Optimizing System For Sperm Quality Evaluation Using Crystalloid Diluent For Pigeons (Columba Livia Domestica), Thanida Sananmuang, Thitirat Singto, Kanyavee Intarak, Pimnipa Sanansin, Muttana Budsasom, Arrita Kongthip, Suwarak Wannaratana

The Thai Journal of Veterinary Medicine

Semen quality is a crucial factor for predicting fertility and hatchability in several avian species including pigeons. To evaluate pigeon semen quality, prior dilution of semen was required due to its small volume with highly concentrated spermatozoa. Conventional crystalloid solutions—Normal saline (NSS) and Ringer’s solution (LRS) are common diluents for such a task; however, the unoptimized dilution rate of such diluents was a major cause contributing to inaccurate semen quality evaluation. Due to the lack of such knowledge in pigeons this study aimed to optimize the dilution rate of Normal saline (NSS) and Ringer’s solution (LRS) for pigeon semen quality …

Testicular Gene Expression Of Anti-Müllerian Hormone, Androgen Receptor And Inhibin Alpha Subunit In Porcine Cryptorchidism, Gunnaporn Suriyaphol, Shanaporn Leelakajornkit, Wipa Sripodok, Wirakan Kallayanathum, Arunthip Komonpunporn, Nutthee Am-In, Sirinun Pisamai, Nathamon Yimpring

The Thai Journal of Veterinary Medicine

Cryptorchidism, one of the common congenital abnormalities in boars, results ina boar taint in the carcass and economic loss. However, knowledge of gene expression of hormones controlling the testicular descent process, such as anti-Müllerian hormone (AMH), androgen receptor (AR) and inhibin alpha subunit (INHA), in porcine testicular cryptorchidismis still limited. The present study aimed to investigate the gene expression profiles of AMH, ARand INHAin undescended testes (UDT) and descended testes (DT) in unilateral cryptorchid pigs of suckling (12 weeks of age), nursery (6weeks of age) and growing-finishing pigs (1520 weeksof age) and in normal testes (NT) at 12 weeks of …

Sequence And Phylogenetic Analysis Of The Vp1 Segment Of Chicken Infectious Anemia Virus (Ciav) During An Outbreak In Palestine, Ibrahim Alzuheir, Nasr Jalboush, Adnan Fayyad, Mohammad Manassra

The Thai Journal of Veterinary Medicine

In this study, chicken infectious anemia virus (CIAV) was detected in two unvaccinatedcommercial broiler flocks, showing clinical signs relevant to Chicken infectious anemia (CIA) disease. The clinical signs included subcutaneous hemorrhage, depression and death. Necropsy findings were pale liver, severe atrophy of bursa of the Fabricius and thymus and discoloration of the bone marrow. DNA was extracted from the livers of the infected birds and the VP1 gene was detected by polymerase chain reaction. The virus strains were detected and genetically characterized in almost the whole VP 1 gene (1338 base pairs). The nucleic acid analysis showed that the two …

Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu

The Thai Journal of Veterinary Medicine

Salmonellaspp. can be widely spread by air or contaminated food. There are sixcommon pathogenic Escherichia coli whichaccount for 4–8% of all E. coli pathogens. Amongstthem, E. coliO157 is the most commonlyknown. In this study, we investigated the prevalence of diarrhoeapathogens, referred to as Salmonellaspp.andEscherichia coli(E. coli), in pet dogs in western Taiwan using the conventionalpolymerase chain reaction (PCR). The related analysis between the prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. Faecalsamples were collected from240dogs which had symptoms of diarrhoeaand had been examined byveterinaryhospitals from January 2015 to January 2017. The …

Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk

The Thai Journal of Veterinary Medicine

True hermaphrodites are animals with both male and female gonads that have concurrently developed in the same individual. The incidence of true hermaphroditism is moderately higher in pigs than in other domestic animals. The aim of this study was to describe the genital organs of gilts culled due to anestrus with true hermaphrodites by gross and histological analysis. Three anestrus gilts were categorized as unilateral true hermaphrodites, having one ovotestis and one ovary, while three other gilts were bilateral true hermaphrodites with two ovotestes. The anomalous structures of the female genital organs, such as oviductal aplasia and hyperclitoris were found …

Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile

The Thai Journal of Veterinary Medicine

A mature intracranial teratoma was identified in a 5-month-old pet rabbit showing a sudden onset of obtundation, recumbency, flaccid tetraplegia, and hyporeflexia. A space-occupying suprasellar, irregular cystic mass extending from the thalamus to the brainstem was observed at necropsy. Histologically, the tumor was composed of irregular, multilocular cysts lined by a thin wall and interposed tissue where a heterogeneous collection of structures including adipose and myxomatous tissues, cartilage, glandularand ductal structures, skeletal muscle, nervous tissue, nerve fascicles, sebaceous glands, and exocrine pancreatic cells were recognized. Immunohistochemistry showed labeling of the diverse tissues with cytokeratin, vimentin, smooth muscle actin, GFAP, NSE, …

Hyperdynamic Circulatory State In A Dog With Leptospirosis: A Case Report, Numfa Fungbun, Naruepon Kampa, Supranee Jitpean, Nitaya Boonbal

The Thai Journal of Veterinary Medicine

A hyperdynamic circulatory state is a common consequence of portal vein hypertension. Interestingly, this case report demonstrates acute canine leptospirosis manifesting with abdominal effusion, which it is focused ona hyperdynamic circulatory state, caused by portal hypertension. Severe acute kidney injury caused by leptospirosis in this case was hypothesized to be aggravated by renal hypoperfusion. This condition was noticed to occur in a similar way to hepatorenal syndrome (HRS) in humans. Briefly, a dog with leptospirosis manifesting with abdominal effusion which was diagnosed to be the consequence of hyperdynamic circulatory state caused by hepatopathy and severe acute kidney injury. Peritoneal dialysis …

Infection Of Subgroup J Avian Leukosis Induces Erythroblasts And Lymphoblasts In Chinese Local Breed Chickens, Jie Yang, Ziqiang Cheng, Chengui Li, Guihua Wang

The Thai Journal of Veterinary Medicine

Anaemia, emaciation, increased mortality and decreased egg production were observed in 21 birds in a Chinese local breed flock infected naturally at 240 days old. Macroscopically, the spleen and kidneys were enlarged. There were diffuse, multifocal white foci on the surface of the liver, lungs and ovaries. Histopathological examination demonstrated typical lymphosarcoma cells and erythroblasts in the lungs, spleen, liver, kidneys and ovaries. The results of polymerase chain reaction (PCR) showed that avian leukosis virus subgroup J(ALV-J) was positive in the liver, spleen and ovaries. Immunofluorescence assay results further confirmed the presence of ALV-J antigen in the liver, kidneys and …

Twin Pregnancy Complicated By Intrauterine Death Of One Fetus And Herniated Bowel Loops Over Gravid Uterus, Malika Hameed, Khalid Ahsan

Department of Anaesthesia

No abstract provided.

Executive Summary Of The Early-Onset Breast Cancer Evidence Review Conference., David Chelmow, Mark D Pearlman, Amy Young, Laura Bozzuto, Sandra Dayaratna, Myrlene Jeudy, Mallory E Kremer, Dana Marie Scott, Julia Sage OʼHara

Department of Obstetrics and Gynecology Faculty Papers

The Centers for Disease Control and Prevention launched the Bring Your Brave campaign to increase knowledge about early-onset breast cancer, defined as breast cancer in women aged 18-45 years. The American College of Obstetricians and Gynecologists convened a panel of experts in breast disease from the Society for Academic Specialists in General Obstetrics and Gynecology to review relevant literature, validated tools, best practices, and practice guidelines as a first step toward developing educational materials for women's health care providers about early-onset breast cancer. Panel members conducted structured literature reviews, which were then reviewed by other panel members and discussed at …

Pregnancy Management In A Patient With Stickler Syndrome., Julie Gomez, Stephanie M. Rice, Mona M. Makhamreh, Huda B. Al-Kouatly

Department of Obstetrics and Gynecology Faculty Papers

BACKGROUND: Stickler syndrome is a collagen disorder that can affect multiple organ systems. It is characterized by ocular abnormalities, hearing loss, midfacial hypoplasia, hypermobility, and joint abnormalities. The phenotypic expression of Stickler syndrome can vary among those affected. Since Stickler syndrome is a collagen disorder, it is possible to expect pregnancy complications similar to those reported in other collagen disorders. To our knowledge, there is only one case report in the literature on the management of pregnancy and delivery of a patient with Stickler syndrome.

METHODS/CASE REPORT: A 37-year-old primigravid woman with a diagnosis of Stickler syndrome presented at 9 …

Phrenic Nerve Injury Secondary To Extracorporeal Membrane Oxygenation In Pregancy: A Case Report, Cheryl Godcharles, Melody Safarzadeh, Emily A. Oliver, Amanda Roman, Huda B. Al-Kouatly

Department of Obstetrics and Gynecology Faculty Papers

Extracorporeal membrane oxygenation (ECMO) is used to provide acute respiratory and/or hemodynamic support to patients with severe, refractory respiratory failure. Phrenic nerve injury with subsequent hemidiaphragm paralysis should be included in the differential diagnosis of pregnant women with persistent hypoxia after ECMO cannulation.

Elagolix Treatment For Up To 12 Months In Women With Heavy Menstrual Bleeding And Uterine Leiomyomas, James A Simon, Ayman Al-Hendy, David F Archer, Kurt T Barnhart, Linda D Bradley, Bruce R Carr, Thomas Dayspring, Eve C Feinberg, Veronica Gillispie, Sandra Hurtado, Jinhee Kim, Ran Liu, Charlotte D Owens, Ozgul Muneyyirci-Delale, Alice Wang, Nelson B Watts, William Schlaff

Department of Obstetrics and Gynecology Faculty Papers

Objective: To investigate the safety and efficacy of elagolix, an oral gonadotropin-releasing hormone antagonist, with hormonal add-back therapy for up to 12 months in women with heavy menstrual bleeding associated with uterine leiomyomas.

Methods: Elaris UF-EXTEND was a phase 3 extension study that evaluated an additional 6 months (up to 12 months total) of elagolix 300 mg twice daily with hormonal add-back therapy (estradiol 1 mg and norethindrone acetate 0.5 mg once daily) in women who completed an initial 6 months of the same treatment in one of two preceding phase 3 studies. The primary endpoint was the percentage of …

Seroprevalence, Biochemical Investigation And Risk Factor Assessment For Hbv & Hcv Infection In Hospital Based Patients Of Islamabad, Pakistan, Narmeen Adnan Rana, Bushra Munir, Nazeer Hussain, Nazia Imtiaz, Muhammad Asif Gondal Dr., Fahed Parvaiz Dr.

Journal of Bioresource Management

Viral hepatitis poses a serious threat to mankind. Hepatitis B and C Virus are blood borne pathogens that affect millions of individually globally. This study was conducted on a hospital-based population in Islamabad, Pakistan over a period of 4 months, utilizing ELISA as the diagnostic technique which suggested a higher seroprevalence rate for both HBV and HCV i.e. 2.07% and 8.24% respectively. A correlational analysis of the biochemical parameters of these individuals with HBV and HCV infection was carried out and the results indicated a positive correlation of HBV with Alkaline Phosphatase (ALP), HCV with Total Bilirubin (TBil) and both …

Renal And Cardiovascular Outcomes Associated With Cannabis Use In Veterans With Advanced Chronic Kidney Disease Transitioning To Dialysis, Praveen Kumar Potukuchi

Theses and Dissertations (ETD)

Background: Legalization of cannabis and its constituents may lead to increased exposure of a higher number of consumers to cannabis use, including those with chronic kidney disease (CKD). We expect increase in cannabis use, especially, in patients with compromised kidney function. However, there is sparse literature on the effects of cannabis use on cardiorenal and mortality outcomes among patients with advanced CKD. Objectives: The main goal of the current study was to examine the effect of cannabis use on renal and cerebrovascular outcomes as well as mortality in patients with advanced CKD. Using a nationwide cohort of US veterans with …

The Scoop, Vol. 7 Issue 3-4, Summer 2020, Health Sciences Library

Volume 7

Latest news and events from the Health Sciences Library in our monthly newsletter for Summer 2020.

Homoeopathy In The Management Of Infectious Diseases: Different Facets Of Its Use And Implications For The Future, Roja Varanasi, Debadatta Nayak

Indian Journal of Research in Homoeopathy

Background: Infectious diseases are a major challenge to humanity and public health at large. Objective: The aim of this study is to conduct a systematic narrative review of the clinical research evidence for homeopathy in the management of infectious diseases. Methods: A comprehensive search of major biomedical databases: Cochrane Database of Systematic Reviews, Pubmed, Core-Hom, AYUSH Research Portal) and other individual journal sites were conducted. Relevant research published between 1999 and 2019 was categorised by study type, clinical condition and causative pathogen. Results: The literature search of two decades (1999–2019) gathered 86 articles: Meta-analysis (n = 1), systematic reviews (n …

Homoeopathic Nosodes, A Neglected Approach For Epidemics: A Critical Review, Debadatta Nayak, Roja Varanasi

Indian Journal of Research in Homoeopathy

Background: Nosodes are the homoeopathic medicines prepared from diseased products of biological origin. These are commonly used in clinical practice for the prophylaxis and treatment of ailments including infectious diseases. Objective: This review aims at the usage of nosodes for the management of epidemics. Methods: The homoeopathic research literature was searched to find examples of the use of nosodes in different ways and to evaluate their role and position in homoeopathic practice with special reference to epidemic diseases. Results: Research evidence for use of the nosodes as prophylactics and for the treatment of various infectious and non-infectious diseases is emerging. …

World Homoeopathy Day 2020: Connecting Digitally During The Adversities Of Covid-19 Lockdown, Deepti Chalia, O Verma, Kavita Bharti

Indian Journal of Research in Homoeopathy

The Central Council for Research in Homoeopathy (CCRH), with support from the Ministry of AYUSH, Government of India, organised its first Webinar on World Homoeopathy Day on 10 April 2020 to commemorate the birth anniversary of the founder of Homoeopathy, Dr Samuel Hahnemann. Considering the need of hour and utilising the advancements of digital platforms, the CCRH decided to organise this webinar focusing on the role of Homoeopathy in COVID-19, for the homoeopathic fraternity and to unite them digitally. The webinar was inaugurated by Shri. Shripad Naik, Minister of State (IC), Ministry of AYUSH; Vaidya Rajesh Kotecha, Secretary, Sh. PK …