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Molecular Characterization Of Histomonas Meleagridis In Clinical Samples Of Chickens From Eastern China, Jinjun Xu, Chanbao Qu, Pin Guo, Zhennan Zhuo, Dandan Liu, Jianping Tao Sep 2016

Molecular Characterization Of Histomonas Meleagridis In Clinical Samples Of Chickens From Eastern China, Jinjun Xu, Chanbao Qu, Pin Guo, Zhennan Zhuo, Dandan Liu, Jianping Tao

The Thai Journal of Veterinary Medicine

Histomonas meleagridis (H. meleagridis) is a protozoan parasite that may cause histomoniasis, a disease of special importance to the poultry industry and public health. The molecular characterization of H. meleagridis in China has not been established. The 5.8S and flanking ITS regions were amplified by polymerase chain reaction from 15 liver samples of chickens which were preliminarily diagnosed with H. meleagridis infection by observing clinical symptoms and macroscopic changes in the organs in Eastern China between 2012 and 2013. The obtained sequences were aligned and compared with other known sequences of H. meleagridis and related protozoan species based on ITS1-5.8S …


Rna Interference Targeting Glycoprotein D Inhibits Infectious Bovine Rhinotracheitis Virus Replication In Mdbk Cells, Lingling Song, Hui Zhang, Hongmei Wang, Peili Hou, Xianzhu Xia, Hongbin He Sep 2016

Rna Interference Targeting Glycoprotein D Inhibits Infectious Bovine Rhinotracheitis Virus Replication In Mdbk Cells, Lingling Song, Hui Zhang, Hongmei Wang, Peili Hou, Xianzhu Xia, Hongbin He

The Thai Journal of Veterinary Medicine

Infectious bovine rhinotracheitis (IBR) is an acute, febrile, contagious disease caused by infectious bovine rhinotracheitis virus (IBRV). IBRV vaccine is considered to be partially effective but not yet completely successful. Therefore, establishing a new antiviral approach is critical. RNA interference (RNAi) has been rapidly developed in recent years as an antiviral therapy for several viruses. This study showed that RNAi could suppress IBRV replication via knockdown of a virion glycoprotein. Two recombinant lentiviral vectors containing short hairpin RNAs (shRNAs) (H1-RNA-121, H1-RNA-304) against the glycoprotein gD of IBRV and a pcDNA3-gD vector containing a FLAG tag were constructed. pcDNA3-gD and the …


Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong Sep 2016

Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong

The Thai Journal of Veterinary Medicine

Our previous study reported the expression of kisspeptin in the preoptic area (POA) and arcuate nucleus (ARC) of the hypothalamus of cycling buffalo cows. In ruminants, POA and ARC are the main hypothalamic nuclei through which kisspeptin acts on the hypothalamic-pituitary-gonadal axis, due to their stimulation of gonadotropin releasing hormone (GnRH) secretion. In many species, kisspeptin activates GnRH neurons via its receptors (KISS1R or GPR54). The present study was designed to explore the expression of KISS1R by GnRH neurons in the POA and ARC hypothalamic nuclei in buffalo cow brains by double-labeling immunohistochemistry. In both the POA and ARC areas, …


Differential Protein Expression In Haemolymph Of First-Stage Red Sternum Mud Crabs (Scylla Serrata), Pattarawadee Srimeetian, Suriyan Tunkijjanukij Sep 2016

Differential Protein Expression In Haemolymph Of First-Stage Red Sternum Mud Crabs (Scylla Serrata), Pattarawadee Srimeetian, Suriyan Tunkijjanukij

The Thai Journal of Veterinary Medicine

Mud crab (Scylla serrata) is one of the important commercial aquatic animals in Thailand. A cause of death in crab farm is red sternum syndrome. The aim of this research was to characterize differential protein expression in the haemolymph of normal mud crabs and first-stage red sternum mud crabs using gel filtration chromatography on a Sephacryl S-200 chromatography column for protein purification. Results showed that the haemolymph of the red sternum mud crabs presented two fractions (F1 and F2) that were not found in that of the normal mud crabs. Subsequently, these peak fractions were separated by two-dimensional electrophoresis (2DE) …


Efficacy Of Different Vaccination Programs Of Live 4/91 Strain Against Thai Qx-Like Infectious Bronchitis Virus In Broiler Chickens, Tawatchai Pohuang, Sarawut Tanasatian, Jiroj Sasipreeyajan Sep 2016

Efficacy Of Different Vaccination Programs Of Live 4/91 Strain Against Thai Qx-Like Infectious Bronchitis Virus In Broiler Chickens, Tawatchai Pohuang, Sarawut Tanasatian, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

Thai QX-like infectious bronchitis virus (IBV) has caused problems in commercial chicken farms as well as small farms in many parts of Thailand. This study investigated the level of protection generated by vaccination with different IBV vaccination programs, 4/91 strain against Thai QX-like IBV. One hundred 1 day old female broiler chickens were randomly divided into 5 groups of 20 chickens each. The chickens in Group 1 were vaccinated with Ma5 strain IBV vaccine at 1 day old and with 4/91 strain IBV vaccine at 14 days old. The chickens in Group 2 were vaccinated with Ma5 and simultaneously with …


Performance Analysis Of The Combined Plug-Flow Anaerobic Digester (Pfad) And Upflow Anaerobic Sludge Blanket (Uasb) For Treating Swine Wastewater In Thailand, Chackrit Nuengjamnong, Pichaya Rachdawong Sep 2016

Performance Analysis Of The Combined Plug-Flow Anaerobic Digester (Pfad) And Upflow Anaerobic Sludge Blanket (Uasb) For Treating Swine Wastewater In Thailand, Chackrit Nuengjamnong, Pichaya Rachdawong

The Thai Journal of Veterinary Medicine

The objective of this research was to analyze the overall performance, especially the removal efficiency, of the combined plug-flow anaerobic digester (PFAD) and upflow anaerobic sludge blanket (UASB) system, at a large swine farm in Thailand. Eighty-four wastewater samples from the digester were collected during the observation period of 302 days. Appropriate influent and effluent regions were selected for wastewater collection. Treatment efficiencies of the system were evaluated by key parameters such as chemical oxygen demand (COD), soluble chemical oxygen demand (SCOD), total solids (TS), total suspended solids (TSS) and total Kjeldahl nitrogen (TKN). In addition, microbiological parameters such as …


Quantitative Method For Detecting Staphylococcus Aureus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Yumiko Itabashi, Yukio Morita, Sumalee Boonmar Sep 2016

Quantitative Method For Detecting Staphylococcus Aureus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Yumiko Itabashi, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

We developed a novel quantitative method for detecting S. aureus using the DOXTM system. A total of 19 strains of S. aureus, and 36 strains of non-S. aureus containing 27 species were examined. The mean positive rate of the high- (103 CFU/ml), medium- (102 CFU/ml), and low-concentration (10 CFU/ml) S. aureus samples was all 100%. The relationship between detection time and bacterial count of the 19 S. aureus had a good linear calibration curve. For the 36 non-S. aureus samples, the mean negative rates for the high-concentration (106 CFU/ml) samples and the medium-concentration (103 CFU/ml) samples were both 94.4% (34/36). …


Ecg Quiz, Chollada Buranakarl, Piyasiri Glangosol, Anusak Kijtawornrat, Saikaew Sutayatram Sep 2016

Ecg Quiz, Chollada Buranakarl, Piyasiri Glangosol, Anusak Kijtawornrat, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Sep 2016

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Nan Choisunirachon Sep 2016

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.


Prevalence Of Clostridium Perfringens Β2-Toxin In Sheep And Goat Population In Punjab, Pakistan, Mudassar Mohiuddin, Zahid Iqbal, Sajjad Ur Rahman Sep 2016

Prevalence Of Clostridium Perfringens Β2-Toxin In Sheep And Goat Population In Punjab, Pakistan, Mudassar Mohiuddin, Zahid Iqbal, Sajjad Ur Rahman

The Thai Journal of Veterinary Medicine

Clostridium perfringens presents persistent threat to small animals in causing moderate to severe enterotoxemia. The pathogenicity of C. perfringens depends on the production of four major toxins. In addition to the major toxins, beta2 (β2) toxin, a minor toxin, is also produced by some strains of C. perfringens. In this study, a total of 107 fecal samples collected from healthy and diseased sheep and goats were cultured and processed for biochemical analysis. On the basis of biochemical characterization, 61 isolates were confirmed as C. perfringens. In order to detect C. perfringens types, multiplex PCR assay was carried out for the …


A Case Of Pyometra With Leiomyoma, Paraovarian Cyst And Sutured Vagina In A Bitch, Murside Ayse Demirel, Duygu Baki Acar Sep 2016

A Case Of Pyometra With Leiomyoma, Paraovarian Cyst And Sutured Vagina In A Bitch, Murside Ayse Demirel, Duygu Baki Acar

The Thai Journal of Veterinary Medicine

The present case report describes a 6-year-old, mixed-breed bitch that was referred due to anorexia, polyuria, polydipsia, and lethargy for 2 weeks. The bitch had been spayed 4 years previously at a different clinic. Vaginal inspection by speculum and cytology was not possible because the vaginal canal had been closed surgically. Stump pyometra was suspected upon ultrasonographic and clinical examination. However, the whole uterus and ovaries were observed during surgery. Thus, the bitch had in fact not been spayed as noted anamnesis, but rather the vagina had been sutured to prevent copulation. Histopathological analysis of the uterus and ovaries revealed …


Perineal Hernia Containing Pregnant Uterus In A Doe, E. Sinem Ozdemir Salci, Baris Guner Sep 2016

Perineal Hernia Containing Pregnant Uterus In A Doe, E. Sinem Ozdemir Salci, Baris Guner

The Thai Journal of Veterinary Medicine

A Saanen Maltese mixed-breed doe, about 5 years old, at last gestation, was presented with a complaint of a gradually enlarging swelling on the caudo-ventral area of the abdomen and proximal part of the udders. In clinical examination, abdominal distension and enlargement, a swelling beginning at the right perineal region and extending to the caudo-ventral area of the abdomen and the dorsal site of the udders, were observed. Two fetus and their organs (extremities) were felt by palpation of the swelling. Pulsations of the yeanlings in the swelling were determined by ultrasonographic examination. Vaginal examination result was normal and cervix …


Prrsv Genetic Variation After The 2010 Hp-Prrsv Introduction In Thailand, Korakrit Poonsuk, Jirapat Arunorat, Nanthiya Iampraphat, Komkrich Teankum, Roongroje Thanawongnuwech Sep 2016

Prrsv Genetic Variation After The 2010 Hp-Prrsv Introduction In Thailand, Korakrit Poonsuk, Jirapat Arunorat, Nanthiya Iampraphat, Komkrich Teankum, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

After the first introduction of porcine reproductive and respiratory syndrome virus (PRRSV) into Thailand, Thai type 1 PRRSV has existed in the Thai swine population and caused subclinical diseases while type 2 PRRSV, which most PRRSV isolates belong to, has caused moderate to severe diseases. Highly pathogenic-PRRSV (HP-PRRSV) has recently emerged in Thailand, causing significant damages since its first introduction in 2010. Genetic characterization of Thai PRRSV isolates collected from several clinically affected swine herds distributed in different geographical locations in 2012 was analyzed using a comparative analysis between 2 hyper-variable regions, NSP2 and ORF5. Eleven selected PRRSV clinical samples …


Inspection Of C-Type Lectin Superfamily Expression Profile In Chicken And Mouse Dendritic Cells, Kaj Chokeshaiusaha, Roongroje Thanawongnuwech, Denis Puthier, Catherine Nguyen Sep 2016

Inspection Of C-Type Lectin Superfamily Expression Profile In Chicken And Mouse Dendritic Cells, Kaj Chokeshaiusaha, Roongroje Thanawongnuwech, Denis Puthier, Catherine Nguyen

The Thai Journal of Veterinary Medicine

C-type lectin superfamily is a cell surface molecule family well-recognized for its role in inducing and tailoring dendritic cell (DC) immune response. Despite its importance in DC targeting and activation, knowledge about C-type lectin family expression in chicken DC is still limited, and thus hinders its utilization for poultry DC vaccine development. In this study, expressions of C-type lectin orthologous gene among available chicken and mouse DC subsets were determined for expression levels and differential expression patterns. Leukocyte microarray datasets and bone marrow-derived DC mRAN-sequencing datasets were pre-processed prior to observation and comparison. Data virtualization revealed variation in C-type lectin …


Ichthyosis Congenita In A Calf: Case Report, Saeed Azimpour, Farhang Sasani, Parham Mottaghian, Amin Anoushepour Sep 2016

Ichthyosis Congenita In A Calf: Case Report, Saeed Azimpour, Farhang Sasani, Parham Mottaghian, Amin Anoushepour

The Thai Journal of Veterinary Medicine

The purpose of this report was to present the clinical and pathological features of ichthyosis congenital observed in a cross-bred Holstein calf. The calf showed regions of alopecia and moderately thickened, fissured skin at birth. The major dermatopathological feature was diffuse lamellar orthokeratotic hyperkeratosis with severe multiple follicular keratosis along with mild acanthosis. The definitive diagnosis was based on history, physical examination, and dermatopathological findings.


Evidence Of Ranavirus In A Green Tree Frog (Litoria Caerulea) In Captive Zoo, Nopadon Pirarat, Angkana Sommanustweechai, Somporn Techangamsuwan Sep 2016

Evidence Of Ranavirus In A Green Tree Frog (Litoria Caerulea) In Captive Zoo, Nopadon Pirarat, Angkana Sommanustweechai, Somporn Techangamsuwan

The Thai Journal of Veterinary Medicine

Ranavirus is one of the causative agents responsible for the rapid decline in amphibian populations worldwide. The objectives of this study were to detect and to genetically characterize ranavirus infection in 36 liver tissues obtained from dead imported zoo amphibians in Thailand. Polymerase chain reaction and molecular phylogenetic analysis was performed based on nucleotide sequences containing major capsid protein gene. Results showed 1 positive sample (~531 bp) from a green tree frog (Litoria caerulea). The virus was highly homologous (98%) and closely related to ranavirus. Our results extend the risk and evidence of ranavirus infection in zoo amphibian in Thailand.


Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk Sep 2016

Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk

The Thai Journal of Veterinary Medicine

Post-partum and lactation are the most complex periods in the swine production chain. Newborn pigs are highly vulnerable due to relatively low body weight at birth and physiological immaturity. Most of the management strategies performed in farrowing houses are oriented to ensure a proper level of colostrum intake by the piglets. Colostrum is essential as an energy source and to provide passive immunity to piglets. Different farrowing supervising protocols have been comprehensively investigated to reduce early mortality as well as to assist newborn piglets in obtaining an optimal amount of colostrum and milk. However, little is known of the benefits …


Detection Of Giant Fibres In Undernourished Fetal Sheep Muscle, Berjan Demirtas Sep 2016

Detection Of Giant Fibres In Undernourished Fetal Sheep Muscle, Berjan Demirtas

The Thai Journal of Veterinary Medicine

During an investigation the occurrence of giant muscle fibres was noted in fetal sheep skeletal muscle. The opportunity was, therefore, taken to clarify the histochemical and ultrastructural characteristics of these fibres and to understand if environmental stress such as maternal undernutrition during gestation affects the occurrence of these giant fibres in fetal sheep muscle. Welsh mountain ewes were randomly assigned to either the control group or the nutrient restricted group. The restricted animals received 50% of their daily nutritional requirement from the time of conception until 70 days of gestation and then 100% of their daily nutritional requirement thereafter. The …


Effectiveness Of Disease Modifying Osteoarthritis Agents And Carprofen For Treatment Of Canine Osteoarthritis, Irin Kwananocha, Monchanok Vijarnsorn, Narudee Kashemsant, Chalermpol Lekcharoensuk Sep 2016

Effectiveness Of Disease Modifying Osteoarthritis Agents And Carprofen For Treatment Of Canine Osteoarthritis, Irin Kwananocha, Monchanok Vijarnsorn, Narudee Kashemsant, Chalermpol Lekcharoensuk

The Thai Journal of Veterinary Medicine

A prospective, randomized, single-blinded study was conducted to evaluate and compare the effectiveness of disease modifying osteoarthritis agents (DMOAAs) and carprofen by using force plate gait analysis and orthopaedic assessment score (OAS) in osteoarthritic dogs. Forty dogs with hip and/or stifle osteoarthritis (OA) were assigned randomly into four treatment groups: PCSO-524, treated with a marine-based fatty-acid compound; GC-ASU, treated with a combination of glucosamine-chondroitin sulphate and avocado/soybean unsaponifiables; CPF, treated with carprofen; and CPF-PCSO, treated with a combination of carprofen and PCSO-524. Each group received the therapeutic agent orally for four weeks. Peak vertical force (PVF), OAS, haematology and blood …


Comparative Proteomic Analysis Of Cow Placentas With Retained Foetal Membranes, Shixin Fu, Yao Liu, Tiantian Nie, Chengyuan Zheng, Xiao Zou, Chunhai Luo, Jianying Liu Jun 2016

Comparative Proteomic Analysis Of Cow Placentas With Retained Foetal Membranes, Shixin Fu, Yao Liu, Tiantian Nie, Chengyuan Zheng, Xiao Zou, Chunhai Luo, Jianying Liu

The Thai Journal of Veterinary Medicine

Retained foetal membrane (RFM) is a common reproductive pathology that harms the health of dairy cows. The aim of this study was to clarify the pathogenesis of RFM and screen for an early clinical diagnostic marker of this disease by using comparative proteomics and bioinformatics. Three RFM placental tissues from three cows and an equal number of normal placental tissues were collected for this study. There were 240 spots showing significant differential expressions of known proteins between the two types of foetal placental tissue samples. Between the two maternal placental tissue samples, there were 214 spots with differentially expressed levels …


Ophthalmology Snapshot, Nalinee Tuntivanich Jun 2016

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


The Goat Semen Quality After Being Frozen Using Albumin And Cholesterol Substituted For Egg Yolk In Semen Extender, Terdchai Kaewkesa, Anucha Sathanawongs, Apichart Oranratnachai, Jureerat Sumretprasong Jun 2016

The Goat Semen Quality After Being Frozen Using Albumin And Cholesterol Substituted For Egg Yolk In Semen Extender, Terdchai Kaewkesa, Anucha Sathanawongs, Apichart Oranratnachai, Jureerat Sumretprasong

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the effect of albumin and cholesterol substituted for egg yolk in goat semen extender after frozen-thawed on semen quality and pregnancy rate. The semen was collected from three bucks by using artificial vagina. The semen was divided into 5 groups; in group 1, seminal plasma was removed and 20% egg yolk extender was added (standard), in group 2, 2% egg yolk extender was added into whole semen (control), in group 3, 4 mg/mL albumin extender was added into whole semen, in group 4, 5 mg/mL cholesterol extender was added into whole semen, …


Seroprevalence And Phylogenetic Analysis Of Toxoplasma Gondii From Domestic Cats, Captive Wild Felids, Free-Range Wild Felids And Rats In Certain Regions Of Thailand, Ruangrat Buddhirongawatr, Kridsada Chaichoun, Siriporn Tungsudjai, Ruenruethai Udonsom, Andrew Thompson, Oangart Mahittikorn, Paron Dekumyoy, Yaowalark Sukthana Jun 2016

Seroprevalence And Phylogenetic Analysis Of Toxoplasma Gondii From Domestic Cats, Captive Wild Felids, Free-Range Wild Felids And Rats In Certain Regions Of Thailand, Ruangrat Buddhirongawatr, Kridsada Chaichoun, Siriporn Tungsudjai, Ruenruethai Udonsom, Andrew Thompson, Oangart Mahittikorn, Paron Dekumyoy, Yaowalark Sukthana

The Thai Journal of Veterinary Medicine

Toxoplasmosis is an important zoonotic disease caused by Toxoplasma gondii, an obligated zoonotic apicomplexan parasite. The infection varies according to geographical areas. This work aimed to study the seroprevalence and genotype of T. gondii infection in domestic, captive and free-range wild felids, and in their small mammal prey, rats (Rattus spp). Two hundred and ninety three sera, received from the 4 individual animal groups in Thailand, were tested using the indirect latex agglutination test (ILAT) for specific antibody detection. The nested-PCR for glycerol-3-phosphate (B1) and bradyzoite surface antigen (SAG4) gene detection was used to detect seropositive animals and PCR product …


Diversity Of Non-Flavobacterium Columnare Bacteria Associated With Columnaris-Like Diseased Fish, Ha Thanh Dong, Vuong Viet Nguyen, Wanniwat Mata, Pattanapon Kayansamruaj, Saengchan Senapin, Dachrit Nilubol, Channarong Rodkhum Jun 2016

Diversity Of Non-Flavobacterium Columnare Bacteria Associated With Columnaris-Like Diseased Fish, Ha Thanh Dong, Vuong Viet Nguyen, Wanniwat Mata, Pattanapon Kayansamruaj, Saengchan Senapin, Dachrit Nilubol, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Numerous yellow and pink pigmented bacterial isolates had been recovered from diseased fish exhibiting columnaris-like disease in tilapia (Oreochromis spp.) farms in Thailand. The predominant species had been identified as Flavobacterium columnare while taxonomic classification of the remaining isolates and their pathogenic potential remain undetermined. An additional yellow bacterial strain had also been obtained from a koi carp sample showing columnaris-like symptoms. To continue our previous work, this study described the identification of ten representatives of unknown culturable non-Flavobacterium columnare bacteria based on a combination of phenotypic characteristics and nucleotides homology of 16S rRNA gene and subsequently investigated their pathogenicity …


Identification Of Competitive Exclusion And Its Ability To Protect Against Campylobacter Jejuni In Broilers, Thotsapol Thomrongsuwannakij, Rungtip Chuanchuen, Niwat Chansiripornchai Jun 2016

Identification Of Competitive Exclusion And Its Ability To Protect Against Campylobacter Jejuni In Broilers, Thotsapol Thomrongsuwannakij, Rungtip Chuanchuen, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study aimed to identify competitive exclusion (CE) isolated from native chickens and organic layers raised under non-antimicrobial usage farms. The protection of CE was tested against Campylobacter jejuni challenges in broilers. Lactobacillus spp., Bacillus spp. and Enterococcus faecium were identified from 50 adult chicken feces of those farms. According to antimicrobial-resistance concerns of European Food Safety Authority (EFSA), the use of CE that is susceptible to antimicrobials is preferred. The numbers of Lactobacillus spp., Bacillus spp. and Enterococcus faecium equal to 195, 93 and 58 strains, respectively, were tested for their minimum inhibitory concentrations (MICs) of 10 antimicrobials. As …


Bactericidal Effects Of Povidone-Iodine And Chlorhexidine Gluconate On Coagulase-Positive Staphylococci, Punpichaya Fungwithaya, Nuvee Prapasarakul Jun 2016

Bactericidal Effects Of Povidone-Iodine And Chlorhexidine Gluconate On Coagulase-Positive Staphylococci, Punpichaya Fungwithaya, Nuvee Prapasarakul

The Thai Journal of Veterinary Medicine

We aimed to evaluate the in vitro bactericidal efficacy of two routine antiseptics used in veterinary hospital, povidone-iodine and chlorhexidine gluconate in isopropanol (CGI),against canine coagulase-positive staphylococci (CoPS). Twenty CoPS were divided into 5 methicillin-resistant Staphylococcus pseudintermedius (MRSP) and 5 isolates each of S. pseudintermedius, S. aureus and S. schleiferi subsp. coagulans defined as methicillin-susceptible CoPS (MSCoPS). The bactericidal efficacy was determined by broth microdilution according to European Standard EN 1656:2000 at concentrations of 0.1, 1 and 10% PI and 0.5, 1 and 2% CGI for 15s, 30s, 45s, 1 min, 3 min and 5 min, respectively. There was no …


Quantitative Method For Detecting Vibrio Parahaemolyticus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar Jun 2016

Quantitative Method For Detecting Vibrio Parahaemolyticus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

This is the first report on the quantitative method for detecting Vibrio parahaemolyticus using the Bio-Theta DOXTM system. Seven V. parahaemolyticus strains and 23 non-V. parahaemolyticus strains were assessed. The rate of positive detection of Vibrio in samples of the 7 V. parahaemolyticus strains at high concentration (105 cfu/ml), medium concentration (103 cfu/ml), and low concentration (10 cfu/ml) was 100%. All 7 V. parahaemolyticus strains displayed good linear calibration curves for detection time relative to bacterial count (r > 0.993). The mean negative rate for the high-concentration (106 cfu/ml) non-V. parahaemolyticus samples was 65.2% (15/23) and that for the medium-concentration (103 …


Urethral Rupture And Leakage Following Prolonged Surgical Removal Of Urethral And Bladder Stones By Episiotomy And Urethrotomy In Female Asian Elephant (Elephas Maximus) : A Case Report, Nikorn Thongtip, Benchapol Lorsunyaluck, Manakorn Sukmak, Sirinart Chaichanathong, Pornchai Sunyathitiseree Jun 2016

Urethral Rupture And Leakage Following Prolonged Surgical Removal Of Urethral And Bladder Stones By Episiotomy And Urethrotomy In Female Asian Elephant (Elephas Maximus) : A Case Report, Nikorn Thongtip, Benchapol Lorsunyaluck, Manakorn Sukmak, Sirinart Chaichanathong, Pornchai Sunyathitiseree

The Thai Journal of Veterinary Medicine

A 46-year-old female Asian elephant (Elephas maximus) with an abnormal ballottement mass in the perineal area was presented. The elephant was unable to urinate via the normal tract. Previously, this elephant was treated for urethral and bladder obstruction caused by stones by episiotomy and urethrotomy. Eight months later, post-operative care was completed. At this point, the incision wound completely closed and the elephant could urinate through the normal tract. However, the elephant was then mated with an adult male elephant. Three weeks later, the female showed signs of the perineal ballottement mass and was unable to urinate. Episiotomy and urethrotomy …


Pulp-Dentin Complex Response To Ru-Hbm1, A Novel Resin Modified Glass Ionomer Cement Prototype, In Deep Cavity Preparation Of Porcine Teeth, Wijit Banlunara, Siriporn Songsiripadapboon, Sirithan Jiemsirilers, Onusa Saravari, Dujreutai Pongkao Kashima, Nonglax Thunyakitpisal, Pasakorn Brikshavana, Phanitnan Srisuwan, Thitima Rupunt, Pasutha Thunyakitpisal Jun 2016

Pulp-Dentin Complex Response To Ru-Hbm1, A Novel Resin Modified Glass Ionomer Cement Prototype, In Deep Cavity Preparation Of Porcine Teeth, Wijit Banlunara, Siriporn Songsiripadapboon, Sirithan Jiemsirilers, Onusa Saravari, Dujreutai Pongkao Kashima, Nonglax Thunyakitpisal, Pasakorn Brikshavana, Phanitnan Srisuwan, Thitima Rupunt, Pasutha Thunyakitpisal

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the response of the pulp-dentin complex to Research Unit-Herbal Medicine, Biomaterial and Material 1 (RU-HBM1), a resin-modified glass ionomer (RMGI) cement prototype, in deep cavity of porcine teeth compared with commercial RMGIs. Six 14-week-old pigs were used in this study. Deep class V cavity was created on the buccal surface of canine, premolar, and molar teeth. The teeth were randomly lined with the design cement bases: RU-HBM1, Vitrebond (VB), or GC-Gold Label Light-Cured Universal Restorative (GC). After that, the cavity walls were treated with 30% phosphoric acid and washed. Then, a bonding …