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Study Of Genetic Variation Of Captive Asiatic Golden Cat (Pardofelis Temminckii) In Thailand Using Domestic Cat (Felis Catus) Microsatellite Markers, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr
Study Of Genetic Variation Of Captive Asiatic Golden Cat (Pardofelis Temminckii) In Thailand Using Domestic Cat (Felis Catus) Microsatellite Markers, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr
The Thai Journal of Veterinary Medicine
Domestic cat (Felis catus) microsatellite markers were used to determine genetic diversity in 17 captive Asiatic golden cats (Pardofelis temminckii, syn. Catopuma temminckii). Allele numbers, genotype numbers, genetic diversity, heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were observed in each marker. The average values of genotype number, allele number, expected heterozygosity, observed heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were 6.12, 4.71, 0.70, 0.29, 0.65 and 0.62, respectively. These results revealed a reduction in the genetic diversity as shown by the lower value for the observed heterozygosity compared to the expected heterozygosity and the high value …
Evaluation Of Amelogenin And Zinc-Finger Loci For Sex Identification In Captive Felids, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr
Evaluation Of Amelogenin And Zinc-Finger Loci For Sex Identification In Captive Felids, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr
The Thai Journal of Veterinary Medicine
The amelogenin and zinc-finger regions were used for sex determination in six species of felids: Felis catus (Persian cat), Felis chaus (jungle cat), Prionailurus bengalensis (leopard cat), Pardofelis temminckii (Asiatic golden cat), Panthera tigris (tiger) and Prionailurus viverrinus (fishing cat). The amelogenin region in all six felids could be successfully amplified and gave a heterozygous genotype in males and a homozygous genotype in females, except for leopard cats which showed a heterozygous genotype in both sexes. The PCR products from the zinc-finger region could be amplified in Persian cats, Asiatic golden cats, tigers and fishing cats. In the Persian cat, …