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Full-Text Articles in Medicine and Health Sciences
Advanced Biotechnologies For Wildlife Fertility Preservation, Pierre Comizzoli
Advanced Biotechnologies For Wildlife Fertility Preservation, Pierre Comizzoli
The Thai Journal of Veterinary Medicine
Reproductive biotechnologies are critical tools for saving and maintaining endangered species. Some successes have been reported with the use and integration of artificial insemination (with fresh or frozen-thawed semen) in conservation programs. However, not a single species is currently managed through oocyte freezing or embryo-based technologies. This is primarily due to the lack of knowledge of species biology, as well as inadequate facilities, space, expertise, and funding needed for their successful application. More fundamental studies of animal reproductive biology as well as more fertility preservation options are needed with all parties involved (reproductive technologists, zoo biologists and conservationists) adopting parallel …
Optimization Of Trehalose Concentration In Semen Freezing Extender In Thai Native Chicken Semen, Pachara Thananurak, Thevin Vongpralup, Chamaiporn Sittikasamkit, Kedsirin Sakwiwatkul
Optimization Of Trehalose Concentration In Semen Freezing Extender In Thai Native Chicken Semen, Pachara Thananurak, Thevin Vongpralup, Chamaiporn Sittikasamkit, Kedsirin Sakwiwatkul
The Thai Journal of Veterinary Medicine
The aim of this study was to find optimal concentration of trehalose supplemented to semen extenders (0, 5, 10, and 15 mM). Ejaculates were collected from 24 Thai native cocks (Pradu Hang Dam; 1 year old), then diluted (1:3) and pooled before allocation to treatments. In the experiment, semen was either cooled to 5ºC after dilution or diluted with a pre-cooled diluents before cooling to 5ºC. Semen freezing was performed in liquid nitrogen vapor at -135ºC and -35ºC, with 6% DMF, and compared for sperm qualities. After thawing, it was measured for total sperm motility and progressive motile by CASA. …