Medicine and Health Sciences Commons^™

Genetics And Functions Of The Sars Coronavirus Spike Protein, Chad Michael Petit

LSU Doctoral Dissertations

The SARS-Coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. Investigations, described herein, have focused on the genetic manipulation of the SARS-CoV S glycoprotein in order to delineate functional domains within the protein. This was accomplished by incorporating single point mutations, cluster-to-lysine and cluster-to-alanine mutations, as well as carboxyl terminal truncations into the protein and investigating these mutants in transient expression experiments. Mutagenesis of either the coiled-coil domain of the S glycoprotein amino terminal heptad repeat, the predicted fusion peptide, …

The Role Of Urease In The Pathogenesis Of Edwardsiella Ictaluri, Natha Joy Booth

LSU Doctoral Dissertations

An Edwardsiella ictaluri strain with disruption of ureG was identified through the use of signature tagged insertion mutagenesis as being attenuated for virulence in the channel catfish host. Sequencing of the flanking regions surrounding the insert showed that the gene was part of a urease gene complex that included ureE, ureF, ureG, ureD, ureI, and an ammonium transporter homologue. The ureG gene encodes a GTP-binding accessory protein which is thought to function in energy-dependent urease assembly. The ureG mutant strain was found to be attenuated for mortality, persistence, and for the ability to establish infection in a competition challenge during …

Matrix Metalloproteinase 3, Matrix Metalloproteinase 13, And Tissue Inhibitor Of Metalloproteinase 1 Concentrations In Normal And Naturally-Occurring Osteoarthritic Canine Stifles, Blaine Allen Burkert

LSU Master's Theses

Osteoarthritis is arguably the most common ailment in both dogs and people in the developed World. Treatment for osteoarthritis is currently symptomatic. Development of therapies designed at stopping the progression of osteoarthritis, require a method of evaluating efficacy. Objective analysis by measuring joint metabolism via clinical trials of the cross-over design is currently not possible with the methods utilized. Extracellular matrix degradation is a hallmark of osteoarthritis. The matrix metalloproteinases are major degradative enzymes. There are currently no commercially available assays to measure canine matrix metalloproteinases. Human and canine matrix metalloproteinases are highly homologous. The use of antibodies and assays …

The Influence Of Transdermally Administered Fentanyl On Isoflurane Requirements In Normothermic And Hypothermic Dogs, Diane Wilson

LSU Master's Theses

Intraoperative reductions in serum fentanyl levels in dogs with induced moderate hypothermia and transdermal fentanyl patches (TDF) in place has been documented. The impact of such reductions has not been evaluated nor has the anesthetic sparing effect of the TDF. Reductions in serum levels may be the result of either a decreased uptake of fentanyl from the dermal depot, or a biophase shift. The objective of this investigation was to determine whether the minimum alveolar concentration (MAC) of isoflurane was altered in the presence of TDF in normothermic and hypothermic dogs. Six mature, healthy, mixed breed dogs were anesthetized on …

Urokinase And Urokinase Receptor In The Urinary Tract Of The Dog, Trina Racquel Bailey

LSU Master's Theses

Urokinase (uPA) and urokinase receptor (uPAR) are present in the urinary tract of people, mice, rats and cows. The presence of urokinase and urokinase receptor in the urinary tract of dogs has not been documented. Documenting the presence of uPA and uPAR in the urinary tract of healthy dogs will help determine the role these proteins play in pathology of the urinary tract in further research. Urine was collected from fifty-four healthy dogs. A fluorescence assay was developed using the fluorogenic substrate Z-Gly-Gly-Arg-AMC, HCl. This assay documented presence of active uPA-like protein in concentrated urine. A direct sandwich ELISA assay …

The Use Of A Whole Genome Scan To Find A Genetic Marker For Degenerative Suspensory Ligament Desmitis In The Peruvian Paso Horse, Diane I. Strong

University of Kentucky Master's Theses

Degenerative suspensory ligament desmitis (DSLD) is a debilitating disease of connective tissues seen in many breeds but has become prevalent in the Peruvian Pasohorse. DSLD is believed to be a genetic disorder caused by one primary founder and most likely has a recessive mode of inheritance although a dominant or co-dominant mode of inheritance has not been ruled out.

A genome scan using 259 microsatellite markers was used to test for linkage disequilibrium between one or more markers and DSLD. Two groups of Peruvian Pasohorses were selected from one population including the US and Canada. The only difference between the …

Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase and has multiple functions residing in its different domains. In the present study, we examined the role of the hypervariable hinge region of P protein in viral RNA synthesis and recovery of infectious VSV by using transposon-mediated insertion mutagenesis and deletion mutagenesis. We observed that insertions of 19-amino-acid linker sequences at various positions within this region affected replication and transcription functions of the P protein to various degrees. Interestingly, one insertion mutant was completely defective in both transcription and replication. Using …

Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo

Jay Reddy Publications

Female B10.S mice are highly resistant to proteolipid protein (PLP) 139–151-induced experimental autoimmune encephalomyelitis (EAE) and depletion of PLP 139–151- reactive CD4+CD25+regulatory T (Treg) cells can slightly increase their EAE susceptibility. Although male B10.S mice are moderately susceptible to EAE, we report that depletion of Treg cells in male B10.S mice before immunization with PLP 139–151 renders them highly susceptible to severe EAE with more CNS neutrophil infiltrates than nondepleted controls. Increased susceptibility is associated with an enhanced PLP 139–151-specific T cell response and greater production of IFN-γ, IL-6, and IL-17. Male CD4+CD25+ effector cells depleted of Treg cells proliferate …

Latency-Related Gene Encoded By Bovine Herpesvirus 1 Promotes Virus Growth And Reactivation From Latency In Tonsils Of Infected Calves, Sandra Perez, Melissa Inman, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infected calves. Dexamethasone (DEX) consistently induces reactivation from latency, and viral gene expression is detected in TG and tonsils. In sensory neurons of latently infected calves, the latency-related (LR) gene is abundantly expressed and is required for reactivation from latency. In the present study, we compared the abilities of wild-type (wt) BHV-1 and a strain …

Factors Associated With The Presence Of Coliforms In The Feed And Water Of Feedlot Cattle, Michael W. Sanderson, Jan M. Sargeant, David G. Renter, Dicky Dee Griffin, Robert A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

The objective of this study was to investigate coliform counts in feedlot cattle water and feed rations and their associations with management, climate, fecal material, and water Escherichia coli O157 using a cross-sectional study design. Coliform counts were performed on feed samples from 671 pens on 70 feedlots and on water samples from 702 pens on 72 feedlots in four U.S. states collected between May and August 2001. Management and climate factors were obtained by survey and observation. Month of sampling (higher in May and June), presence of corn silage in the ration (negative association), temperature of the feed 1 …

Serologic Survey Of Select Infectious Diseases In Coyotes And Raccoons In Nebraska, Richard Bischof, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

To obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in Nebraska, sera were collected from 67 coyotes (Canis latrans) and 63 raccoons (Procyon lotor)) from November 2002 through January 2003. For coyotes, antibodies were detected against canine distemper virus (CDV, 61%), Francisella tularensis) (32%), Rickettsia rickettsi) (13%), and flaviviruses (48%). None of the coyote sera had antibodies to Borrelia burgdorferi, Brucella canis), or six serovars of Leptospira interrogans). Because serologic cross-reactivity exists among flaviviruses, 14 sera from flavivirus-positive coyotes were also tested for St. Louis …

Development And Pathogenesis Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, Eric P. Hoberg, L. Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Recently, the protostrongylid nematode Parelaphostrongylus odocoilei has been reported in a new host species, thinhorn sheep (Ovis dalli). For the first time, we completed the life cycle of P. odocoilei in three Stone’s sheep (O. dalli stonei) and two thinhorn hybrids (O. dalli stonei × O. dalli dalli), each infected with 200 third-stage larvae from slugs (Deroceras laeve). The prepatent period ranged from 68 days to 74 days, and shedding of first-stage larvae (L1) peaked at >10,000 L1 per gram of feces between 90 and 110 days postinfection. A total of 75, …

Effect Of Certain Factors On Productive And Reproductive Efficiency Traits And Phenotypic Relationships Among These Traits And Repeatabilities In West Anatolian Holsteins, Mustafa Tekerli̇, Mustafa Gündoğan

Turkish Journal of Veterinary & Animal Sciences

The present investigation was undertaken to study certain factors affecting production efficiency, milk production and reproduction and to estimate the repeatabilities and phenotypic correlations of these traits in Holsteins maintained at different farms in western Anatolia over 3 years (1990 - 1993). The data consisted of 506 first and 232 second lactations of 525 cows. Three production efficiency traits (milk yield per day of lactation length (MYPDLL), milk yield per day of calving interval (MYPDCI) and milk yield per day of age at second calving (MYPDASC)), one production trait (lactation yield (LY)), and one reproduction trait (calving interval (CI)) were …

Heritabilities Of Tonic Immobility And Leucocytic Response In Sire And Dam Layer Lines, Özge Altan, Petek Settar, Yakut Ünver, Meti̇n Çabuk

Turkish Journal of Veterinary & Animal Sciences

Fearfulness reaction was examined using tonic immobility (TI) response and differential leucocyte counts as physiological indicators of distress from sire and dam brown layer lines. The study was performed on 20 male and 131 female chickens from the sire line and 24 male and 116 female chickens from the dam line. The duration of TI, the time interval until the bird righted itself, and the number of inductions (15 s periods of restraint) necessary to attain TI were recorded. If TI could not be induced after 5 attempts, a score of 0 was recorded. After the TI test, blood samples …

An Abattoir Survey Of Liver And Lung Helminthic Infections In Local And Imported Sheep In Jordan, Anwar Maraqa, Zuhair Sami Amr, Lina Rifai, Walid Al-Melhim

Turkish Journal of Veterinary & Animal Sciences

The livers and lungs of 5596 sheep (443 local, 473 Romanian and 4680 Australian) slaughtered in Amman Central Abattoir during November-December 1999 were examined in routine meat inspection procedures for helminths. Dictyocaulus filaria, hydatid cyst, Fasciola hepatica and Dicrocoelium dendriticum were recovered from the examined sheep with variable prevalences. Dicrocoelium dendriticum is reported from the livers of imported sheep in Jordan for the first time.

Effect Of Single Dose Cadmium Chloride Administration On Oxidative Stress In Male And Female Rats, Haki̇ Kara, Fi̇kret Karataş, Hali̇t Canatan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of single dose cadmium chloride (CdCl_2) administration on the possible development of oxidative stress by measuring malondialdehyde (MDA) levels in the liver, kidney and blood serum of male and female rats. For this purpose 80 animals (14-16 weeks old) were initially divided into two 2 groups according to sex, each containing 40 rats. Then both were divided into 5 equal treatment groups [0 (control) 0.5, 1, 2 and 4 mg/kg CdCl_2 body weight by subcutanous injection, respectively]. The blood serum and tissues of the liver and kidney were collected after 24 …

Performance Testing Studies And The Selection Of Hasmer, Hasak, Hasiv And Linmer Crossbreed Sheep Types: Ii. Pre-Weaning Growth, Mehmet Emi̇n Teki̇n, Mehmet Gürkan, Osman Karabulut, Hüseyi̇n Düzgün

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the pre-weaning growth of crossbreed types. Fifteen genotypes (F_1 and B_1) were obtained by crossbreeding in the Animal Research Institute in Konya from 1989 to 1996. These 15 genotypes were classified in 4 appropriate genotype names: Hasmer, Hasak, Hasiv and Linmer. GLM and Tukey's HSD tests were used for statistical analyses. Year, genotype, sex, birth type, and dam's age were introduced into the model as fixed factors and birth weight was introduced as a covariate along with year x genotype interaction. While the effects of year, dam's age, birth type, and sex on birth …

The Possibilities Of Twice-Yearly Lambing Of Awassi Sheep Ewes Without Using Hormones In An Organic Animal Production System, Ümi̇t Yavuzer

Turkish Journal of Veterinary & Animal Sciences

This goal of the present research was to determine the possibility of twice-yearly lambing in Awassi sheep. The research was appropriate in terms of ecological agriculture, animal breeding applications, and organic agricultural production with no impact on their health or behavior, or exposure to environmental conditions such as exogenous hormone applications. The research was carried out after the February 2001 lambing season. Rams were introduced to the ewe flock while the lambs were weaning. The sheep's giving birth in August 2001 indicated that they had become pregnant while the lambs were weaning. The fertility rate in the August 2001 birth …

Performance Testing Studies And The Selection Of Hasmer, Hasak, Hasiv And Linmer Crossbreed Sheep Types: Iii. Fattening Performance, Mehmet Emi̇n Teki̇n, Mehmet Gürkan, Osman Karabulut, Hüseyi̇n Düzgün

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to evaluate the fattening performance of Hasmer (31.25% Hampshire Down (HD) + 31.25% German Black Headed Mutton (GBM) + 37.50% Merino), Hasak (31.25% HD + 31.25% GBM + 37.50% Akkaraman), Hasiv (31.25% HD + 31.25% GBM + 37.50% Awassi) and Linmer (50% Lincoln + 50% Merino) types. The research was performed between 1997 and 1999. Potential sires were selected based on their performance test results. The general linear model and Tukey's honestly significant difference test were used for statistical analyses. Year and genotype were introduced into the model as fixed factors and initial weight was introduced …

Prevalence Of Salmonella Serogroups In Chicken Meat, Gülşen Goncagül, Elçi̇n Günaydin, K. Tayfun Çarli

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the prevalence of Salmonella serogroups in chicken meat. A total of 315 skins from the wing part of chicken carcasses were collected from 8 chicken carcass retailers. Salmonella isolation was performed as described in the Bacteriological Analytical Manual of the Food and Drug Administration, Center for Food Safety and Applied Nutrition. Salmonella enterica subsp. enterica Serovar Enteritidis (Salmonella Enteritidis) in Serogroup D was isolated from 27 out of 315 (8.57%) chicken carcass skins from the wing parts. Four out of 45 (8.88%), 3 out of 50 (6.00%), 12 out of 57 (21.05%), …

Sero-Prevalence Of Contagious Ecthyma In Lambs And Humans In Kars, Turkey, Hali̇l İbrahi̇m Gökce, Oktay Genç, Gürbüz Gökçe

Turkish Journal of Veterinary & Animal Sciences

The sero-prevalence of contagious ecthyma (orf) in lambs and humans was studied in the Kars region of Turkey. For this purpose, serum samples were collected from 320 lambs from 5 different districts of the Kars region. Serum samples were also collected from 100 humans at risk of infection. The serum samples were tested for the presence of antibodies to orf virus by enzyme-linked immunosorbent assay (ELISA). The results showed that 52.81% and 5% of the serum samples collected from lambs and humans, respectively, were seropositive for orf virus. These results indicate that contagious ecthyma is a prevalent infection in lambs …

Early Morphological Changes After Total Gastrectomy In The Rat Pancreas, Serpi̇l Ünver Saraydin, Emel Koptagel

Turkish Journal of Veterinary & Animal Sciences

Total gastrectomy is widely used as a major surgical treatment for gastric cancer. The aims of this study were to investigate the possible effects of total gastrectomy on pancreas morphology, and to determine the immunolocalisation of chromogranin A (Cg A) and synaptophysin (SYP) antigens, described as specific tumour markers for neuroendocrine tumours. Ten rats underwent total gastrectomy via oesophago-duodenostomy. After a month the rats were killed, and tissue samples were taken from the pancreas, processed for light microscopy and stained with haematoxylin-eosin. In addition, 5-7 µm thick paraffin embedded tissue sections were immunohistochemically stained with Cg A and SYP. The …

The Histochemical And Ultrastructural Structures Of Avian Latissimus Dorsi Muscle Fiber Types And Changes In Them Caused By Water Copper Level, Fati̇me Geyi̇koğlu, Özgen Vuraler, Aysel Temelli̇

Turkish Journal of Veterinary & Animal Sciences

The histochemical and ultrastructural effects of water Cu on the anterior latissimus dorsi (ALD) muscle of 120 newly hatched broilers were investigated in a 45-day experiment. Two doses of water Cu (250 mg Cu/kg water and 500 mg Cu/kg water) were given to the poults. Two distinct categories (IIIA and IIIB) of slow tonic fibers in the control groups were detected by enzyme reactions. The ultrastructure of the Z-line, mitochondrial content and sarcotubular content differed between the 2 fiber types. In the application of 250 mg Cu/kg water, the histochemical characteristics of the IIIB fibers were clearly different from those …

Effects Of Heavy Metal Accumulation On The 96-H Lc_50 Values In Tench Tinca Tinca L., 1758, Syed Lal Shah, Ahmet Altindağ

Turkish Journal of Veterinary & Animal Sciences

The effects of already accumulated heavy metals (Hg, Cd, Pb) in the body of tench on the 96-h LC_50 values of the respective heavy metals were studied. The body concentration of mercury, cadmium and lead was 0.011, 0.32 and 1.59 mg/g respectively, and their 96-h LC_50 values were 1.0, 6.5 and 300.0, ppm, respectively. The general accumulation order of heavy metals and their respective 96-h LC_50 values were Hg< Cd< Pb. It was observed that the fish with lowest body concentration of heavy metal showed the lowest 96-h LC_50 value of the respective heavy metal and vice versa. This close relationship showed that the previous body accumulation of heavy metals has a direct effect on the 96-h LC_50 values of the respective heavy metals and the susceptibility of fish.

The Red Blood Cell Membrane Proteins In Rabbits With Experimental Ketosis, Sefa Çeli̇k, Hi̇lal Karagül

Turkish Journal of Veterinary & Animal Sciences

This study investigated the effects of experimentally induced ketosis on erythrocyte membrane proteins in rabbits by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). The animals were allocated to 2 groups; ketosis and control. The experiment was lasted 13 days. Food was withheld for 5 days in the ketosis group, and then the animals were fed for 8 days. The control group was fed standard rabbit rations. Erythrocyte membrane proteins were isolated from the blood, and analyzed by SDS-PAGE using 10% (w/v) acrylamide monomer. Erythrocyte membrane proteins separated on the polyacrylamide gel were quantified by densitometry. Band 3 and actin on day …

Research On The Factors Affecting Cholesterol Content And Some Other Characteristics Of Eggs In Laying Hens The Effects Of Genotype And Rearing System, Hati̇ce Basmacioğlu, Mustafa Ergül

Turkish Journal of Veterinary & Animal Sciences

The effects of the age of the hen, genotype, rearing system and dietary structure on egg and serum cholesterol content and some other characteristics of eggs were investigated in laying hens. However, only the findings related to genotype and rearing system are given. A total of 840 hens, 21weeks of age and consisting of 400 white layers (Babcock-300) and 440 brown layers (IsaBrown) were used. The 2 genotypes had been housed in different rearing systems, floor pens and cages. Ten different feed groups consisting of controls (I and II), 2% and 4% fiber increment (III, IV, V and VI), 2% …

The Results Of Phacofragmentation And Aspiration Surgery For Cataract Extraction In Dogs, Fatma Eser Özgenci̇l

Turkish Journal of Veterinary & Animal Sciences

Cataract surgery was planned on 41 ERG positive eyes of 25 dogs of which 9 had unilateral and 16 had bilateral cataracts. Phacofragmentation and aspiration surgery was performed on 32 eyes of 20 dogs (and ECCE was performed on 4 eyes and ICCE was performed on 5 eyes of 5 dogs) of different breeds, sexes and ages. Vision restoration and complications were evaluated postoperatively in the short term (4 weeks). Mean age of the 20 dogs was 7.3 years. Stages of cataracts were classified as mature (n: 17), immature (n: 8) and intumescent (n: 7). While the mean phacoemulsification time …

The Prevalence Of Strongyle Infections And Persistent Efficacy Of Pyrantel Embonate, Ivermectin And Moxidectin In Turkish Horses, Veli̇ Yilgör Çirak, Ender Güleğen, Christian Bauer

Turkish Journal of Veterinary & Animal Sciences

Strongyle infection was detected in 68% of 320 horses from 9 farms in western Turkey. Egg counts per gram of faeces (EPG) were more than 950 in half of the animals. The persistent efficacy of 3 different anthelmintics to suppress cyathostome EPG was compared on a horse farm. One group of horses was treated with moxidectin on day 0, and the 2 other groups received pyrantel embonate or ivermectin on days 0 and 70. Pyrantel embonate reduced the EPG by >90% for 2 weeks; thereafter egg counts steadily increased. Ivermectin reduced the EPG by >98% for up to 6 weeks. …

Conjugated Linoleic Acid: Chemical Structure, Sources And Biological Properties, Rahi̇m Aydin

Turkish Journal of Veterinary & Animal Sciences

Conjugated linoleic acid (CLA) is a group of geometrical and positional isomers of linoleic acid (C18:2, cis-9, cis-12). In contrast to linoleic acid, double bonds in CLA are usually located at positions 9 and 11 or 10 and 12 and each double bond can be either in the cis or trans configuration. Meat and dairy products from ruminant animals (such as milk, butter, yogurt and cheese) are the principal natural sources of CLA in the human diet. Egg and meat products from poultry contain less CLA than meat from ruminant animals (0.6 and 0.9 mg/g fat vs. 2.9 to 5.6 …

Immunohistochemical Identification Of Peptide Hormones In The Endocrine Cells Of The Gastrointestinal Tract Of The Oreochromis Niloticus, Berri̇n Gençer Tarakçi, Si̇bel Şi̇mşek Köprücü, Kenan Köprücü

Turkish Journal of Veterinary & Animal Sciences

The endocrine cells of gastrointestinal tract of the Oreochromis niloticus were investigated using immunohistochemical techniques. 8 antisera were tested and 3 immunoreactivities were detected: Serotonin, glucagon and somatostatin immunoreactive cells. Substance P, insulin, gastrin, calcitonin gene related peptide (CGRP) and calbindin immunoreactive cells were not found.