Medicine and Health Sciences Commons^™

Animal Health Matters, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- SDSU Tracks Epizootic Hemorrhagic Disease in South Dakota Cattle [Page] 2- Director's Message: One last time...thanks for the memories [Page] 2- Question and answer Session: Dr. David Zeman [Page] 4- Where the Diagnostic Process Starts: the ADRDL’s Specimen Receiving and Necropsy Sections [Page] 5- Pre-Vet Students and the Necropsy Floor: an Education Unique to SDSU [Page] 6- ADRDL Submission forms now “Save-ablePieces and Parts [Page] 6- Salmonella in Pets Study Extended for Another Year; Samples Requested [Page] 6- Incoming Freshmen with pre-Vet Majors at SDSU, Fall 2012 [Page] 7- Continuing Education Events

Glutaredoxin 2 (Grx2) Knockout Increases Sensitivity To Oxidative Stress In Mouse Lens Epithelial Cells, Hongli Wu, Liren Lin, Frank Giblin, Ye-Sheh Ho, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin belongs to the oxidoreductase family with cytosolic glutaredoxin 1 (Grx1) and mitochondrial gluraredoxin 2 (Grx2) isoforms. Of the two isozymes, the function of Grx2 is not well understood. This paper studied the effect of Grx2 deletion on cellular function using primary lens epithelial cell cultures isolated from Grx2 gene knockout (KO) and wild type (WT) mice. We found that both cell types showed similar growth patterns and morphology, and comparable mitochondrial glutathione pool and complex I activity. Cells with deleted Grx2 did not show affected Grx1 or thioredoxin (Trx) expression but exhibited high sensitivity to oxidative stress. Under treatment …

Controlling Viral Immuno-Inflammatory Lesions By Modulating Aryl Hydrocarbon Receptor Signaling, Tamara Veiga-Parga, A Suryawanshi, B T. Rouse

Tamara Veiga-Parga

Ocular herpes simplex virus infection can cause a blinding CD4+ T cell orchestrated immuno-inflammatory lesion in the cornea called Stromal Keratitis (SK). A key to controlling the severity of SK lesions is to suppress the activity of T cells that orchestrate lesions and enhance the representation of regulatory cells that inhibit effector cell function. In this report we show that a single administration of TCDD (2, 3, 7, 8- Tetrachlorodibenzo-p-dioxin), a non-physiological ligand for the AhR receptor, was an effective means of reducing the severity of SK lesions. It acted by causing apoptosis of Foxp3- CD4+ T cells but had …

Use Of Jakr-Na-Rai (Gynura Divaricata) As A Roughage Source On Growth Performance, Blood Constituent, Blood Glucose And Cholesterol Level In Growing Rabbits, Keerati Keeratikajorn, Navapat Pipatpaitoon, Sunisa Thunyodom, Somkid Khanda, Punyaphat Ittitanawong, Suwanna Kijparkorn

The Thai Journal of Veterinary Medicine

The objective of this study was to investigate Jakr-Na-Rai (Gynura divaricata) as a roughage source on growth performance, blood constituent, blood glucose and cholesterol in growing rabbits. Twenty-four 7-week-old New Zealand White rabbits (12 males and 12 females) were randomly allocated into 3 groups of 8 with equal sex. The rabbits in the first group were fed on commercial feed while the rabbits in the 2nd and 3rd groups were fed the same as first group and supplemented with para grass (Brachiaria mutica) and Jakr-Na-Rai, respectively. The rabbits were raised in individual cages in opened house and received feed and …

Season And Breed Effects On Sperm Production In Prrs Free Boars, Chanyuth Tretipskul, Nutthee Am-In, Padet Tummaruk, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

This study was carried out to investigate sperm production in tropical area of boar stud which has been free from porcine reproductive and respiratory syndrome (PRRS) more than ten years and kept in evaporative cooling system, in relation to season and breed influence. Semen production data from 19,966 ejaculates of 517 boars (164 Duroc, 31 Pietrain, 268 Landrace x Yorkshire: LY, 54 Pietrain x Duroc: PD) were collected. Semen parameters; volume (ml), sperm concentration (x106 sperm/ml) and total number of sperm per ejaculate (x109 sperm/ejaculate) were evaluated. The semen production was shown by month and group in relation to season …

Molecular Detection And Genotype Differentiation Of Feline Coronavirus Isolates From Clinical Specimens In Thailand, Somporn Techangamsuwan, Araya Radtanakatikanon, Suphasawatt Purnaveja

The Thai Journal of Veterinary Medicine

Feline coronavirus (FCoV) infection manifests a wide magnitude of clinical symptoms from nonpathogenic mild enteric infection to pathogenic fatal feline infectious peritonitis (FIP). Based on the in vitro properties and antigenic relationship to canine coronavirus (CCoV), FCoVs are classified into type I and II with different continent preference. To investigate the incidence of FCoV infection among cat populations in Thailand during 2010-2011, clinical specimens (103 bodily fluids from 95 FIP clinically suspected cats and 17 feces from 7 healthy cats living in groups and 10 healthy cats living singly) were analyzed by amplification of the 3’UTR gene. Among them, 46% …

Markedly Different Expression In Mature Bovine Proacrosin Binding Protein (Sp32), Thunchira Thepparat, Suporn Katawatin, Thevin Vongpralub, Monchai Duangjinda, Sompong Thammasirirak, Apirak Utha

The Thai Journal of Veterinary Medicine

Our study investigated proteins in bovine spermatozoa using 2-DE and LC MS/MS and found a markedly different expression in mature bovine proacrosin binding protein (sp32), which is the focus of the present study. Spermatozoa extract from nine fertile Brahman bulls was separated using 2-DE followed by Coomassie brilliant blue staining. From over 600 protein spots, a particular protein spot with two different patterns was detected at 32 kDa. The high relative protein content of spot Y1 (32 kDa pI 5.3, pattern A) was found in six bulls whereas the remaining three bulls expressed two small spots Y1 and Y2 (32 …

Sequence Information, Ontogeny And Expression Analysis Of Complement Component C3 In Walking Catfish Clarias Macrocephalus, Achara Rattanachai, Premruethai Supungul, Prapunsak Srisapoome, Supawadee Poompuang, Anchalee Tassanakajon

The Thai Journal of Veterinary Medicine

Complement system is a powerful host defense mechanism that contributes to both innate and acquired immunities. C3 is the central component of all activation pathways and plays a crucial role in the early immune response. The aim of this present study was to look into ontogeny and expression of C3 in walking catfish Clarias macrocephalus by using molecular techniques. A 4140 bp part of the cDNA encoding the walking catfish C. macrocephalus complement component C3 was obtained by RACR-PCR amplification. The deduced amino acid sequence showed that this partial sequence of C. macrocephalus C3 exhibited 52-64% and 41-45% identity with …

Ophthalmology Snapshot, Tuntivanich Nalinee

The Thai Journal of Veterinary Medicine

No abstract provided.

Effect Of Semen Collection Techniques On Semen Quality And Sperm Motility Parameters In Siamese Fighting Cock (Gallus Gallus), Nathavut Kanatiyanont, Kornchai Kornkaewrat, Piyawan Suthanmapinunt, Anuchai Pinyopummin

The Thai Journal of Veterinary Medicine

Abdominal massage (AM) is a common method for semen collection in avian species. There are few data concerning electro-ejaculation (EE) in this species. This experiment was carried out to study the effect of semen collection techniques (AM and EE) on semen quality and sperm motility parameters in three Siamese fighting cocks (Gallus gallus). EE was done under general anesthesia by isoflurane vaporizer. In experiment 1, either low voltage (5- 10 volts) or high voltage (5-30 volts) protocols for EE stimulation was applied. Ejaculations were obtained from all attempts (n= 4) in both protocols. High voltage protocol gave greater percentage of …

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong

The Thai Journal of Veterinary Medicine

No abstract provided.

Expression Patterns Of Cell Adhesion Molecules In Bovine Preimplantation Embryos Cultured In Vitro, Anucha Sathanawongs, Korakot Nganvongpanit, Supamit Mekchay

The Thai Journal of Veterinary Medicine

This study aimed to investigate gene expression patterns throughout preimplantation embryos and their comparative expression in morphological quality blastocysts using real-time RT-PCR. Bovine oocytes underwent maturation process, fertilized and were cultured in vitro. Degree of fragmentation and differentiation were the criteria to categorize day-7 blastocysts as of good and poor quality. All of the embryos from each developmental stage were used to quantify transcript abundance. It was found that E-cadherin was highly expressed in immature and mature oocytes, then it decreased at 2- to 16-cell stages, and later increased in morula and blastocyst stages. The β-catenin transcript was highly abundant …

Development Of Semi-Nested Pcr For Detection Of 16s Rrna Gene Of Mycoplasma Hyosynoviae, Pacharee Thongkamkoon, Watcharachai Narongsak, Metta Makhanon, Kobayashi Hideki, Koshi Yamamoto

The Thai Journal of Veterinary Medicine

In Thailand, some breeders in pig farms have had osteoarthritis lesions for which M. hyosynoviae might be one of the causative agents. Therefore, the semi-nested polymerase chain reaction assay was developed for detection of Mycoplasma hyosynoviae in organs of pigs in Thailand using three oligonucleotide primers specific to 16S rRNA gene of M. hyosynoviae. The detection limit of purified DNA was 10 femtogram per reaction and of the simulated lung sample was 103 CFU per gram of sample. Preliminary study for tonsil-carrier state of M. hyosynoviae in slaughtered pigs revealed the presence of M. hyosynoviae in 5 out of 10 …

Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in …

Efficacy Of Different Vaccination Programs Against Newcastle Disease Virus Challenge In Broiler Chickens, Jiroj Sasipreeyajan, Tawatchai Pohuang, Nida Sirikobkul

The Thai Journal of Veterinary Medicine

The present study was designed to determine the protection afforded by different vaccination programs against the virulent Newcastle disease virus (vNDV). The chickens were divided into 7 groups, 40 chickens in each. Group 1 did not receive any vaccine and served as a negative control group. Groups 2-7 were vaccinated with different vaccination programs against vNDV. At 1 day old, the chickens in all the vaccinated groups were inoculated with live attenuated vaccine, Ulster strain, followed by different inactivated vaccines including 0.1 ml/chicken of inactivated vaccine A in groups 2 and 4, 0.1 ml/chicken of inactivated vaccine B in groups …

Survey Of Articular Cartilage Injury Of Stifle Joints In 323 Dogs Presented For Surgery In Chiang Mai, Thailand, During, Korakot Nganvongpanit, Terdsak Yano

The Thai Journal of Veterinary Medicine

The purpose of this study was to report the incidence of articular cartilage injury of the stifle joint in 323 dogs found during surgery at orthopedic clinics in Chiang Mai, Thailand, from October 2006 to July 2012. A total of 44 dogs (13.6%) had cartilage lesions. Surgical operations in this study included the corrections of anterior cruciate ligament (ACL) rupture (14.2%), distal femur fracture (15.2%), and patellar luxation (66.6%), and the removal of the IM pin from the tibia (4%). Cartilage injury was found in 1 of the 49 dogs with ACL rupture (2.17%, OR= 0.124); 3 of the dogs …

Risk Factors For Feline Lower Urinary Tract Diseases In Thailand, Rosama Pusoonthornthum, Pinit Pusoonthornthum, Carl A. Osborne

The Thai Journal of Veterinary Medicine

Feline lower urinary tract diseases (FLUTD) is a diagnostic term for cats with hematuria, dysuria, pollakiuria, and partial or complete urethral obstruction. The aim of the present study was to identify risk factors for cats with LUTD. Cats with LUTD were evaluated by history, physical examination, urinalysis, radiography, and contrast radiography. Clinically normal cats consisted of vaccinated cats without clinical signs of LUTD admitted to the same veterinary hospital. Cats with a history of urinary tract disease, and those received special treatment for LUTD were excluded. All cats' owners filled out standardized questionnaires about breed, gender, age, environmental factors, and …

Report Of Lawsonia Intracellularis Infection In Dogs By Polymerase Chain Reaction, Sariya Asawakar, Suphot Watanaphansak, Tanong Asawakarn

The Thai Journal of Veterinary Medicine

The purpose of this study was to survey Lawsonia intracellularis in dogs in Thailand by using Polymerase chain reaction technique. Lawsonia intracellularis is obligatory intracellular bacterium which causes proliferative enteropathy in animals, including dog. Fecal samples from 48 dogs were collected from Bangkok, Pathumthani and Nakhon Ratchasima and tested for L. intracellularis by PCR technique. The dogs were classified into two groups according to the presence or absence of diarrhea. In the group of dogs with diarrhea (n= 11), 6 dogs were positive (54.4%) and in the group without signs of diarrhea (n= 37), 15 dogs were positive (40.5%). The …

Effects Of Semen Extender On Motility And Movement Patterns Of Frozen-Thawed Eld’S Deer (Cervus Eldii) Spermatozoa, Sukumal Rittem, Daraka Thongthainun, Wanlaya Tipkantha, Boripat Siriaroonrat, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

Two semen samples of Siamese Eld,s deer (Cervus eldii siamensis: SED) and seven semen samples of Thamin Eld,s deer (Cervus eldii thamin: TED) were collected by using electroejaculation. Each ejaculate was diluted and cryopreserved with 2 extenders; 1) TRIS; and 2) modified BF5F supplemented with 5% final glycerol concentration. The movement patterns of frozen-thawed spermatozoa were assessed using computer-assisted sperm analysis (CASA). In TED, the results revealed a significantly higher (p< 0.05) percentage of total motility (%TM), percentage of progressive motility (%PM), track speed (VCL) and lateral amplitude (ALH) in TRIS extender. The results also revealed a significantly low (p< 0.05) progressive velocity (VSL), straightness (STR) and linearity (LIN) in TRIS extender. However, for path velocity (VAP) and beat frequency (BCF), there were no differences between both extenders. In SED, sperm motility and sperm movement parameters did not show differences between TRIS and modified BF5F extenders. Findings from this study will aid in Eld’s deer semen banking and artificial insemination success in the future.

Application Of Real-Time Polymerase Chain Reaction For Quantitative Detection Of Chicken Infectious Anemia Virus, Niwat Chansiripornchai, Wisanu Wanasawaeng, Nantawan Wongchidwan, Supawadee Chaichote, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

The present study aimed to develop a quantitative method for the detection of chicken infectious anemia virus (CIAV). A real time polymerase chain reaction (real-time PCR) was developed to determine CIAV. The developed real-time PCR was a rapid and reliable quantitative technique for CIAV DNA compared with the conventional PCR. The appropriate cut-off was at the 40th cycle. Results indicated that the CT value of 39.62 was shown at the 10-9 dilution or 0.454 fg/μl or 3.86 x 103 DNA copies. This data suggests that the limit of detection for real-time PCR is 0.454fg/μl. The quantitative real-time PCR will be …

Histology Of Extrahepatic Bile Ducts In The Iranian Chukar Partridge (Alectoris Chukar), Behzad Mobini, Kaveh Shirani Faradonbeh

The Thai Journal of Veterinary Medicine

A study on the histology of the extrahepatic bile ducts was conducted in eight female and eight male twentyweek- old healthy Iranian partridges (Alectoris chukar). The extrahepatic bile ducts possessed tunica mucosa, muscularis and serosa. The tunica mucosa of the extrahepatic bile ducts was lined by non ciliated simple columnar epithelium. The apical cytoplasm of epithelial cells was covered by a continuous striated border of microvilli. The goblet cells were absent in epithelium. Tunica mucosa formed some simple folds lining with tall columnar epithelium. The lamina muscularis mucosa was absent. All epithelial cells of the bile ducts reacted with acid …

Ecg Quiz, Chollada Buranakarl, Monkon Trisiriroj, Thanusorn Phakhawambodee

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound-Guided Transvaginal Follicular Aspiration And Development Of Vitrified-Thawed Thai Indigenous Beef Cattle (Bos Indicus) Oocytes After In Vitro Fertilization, Denpong Sakhong, Thevin Vongpralub, Suporn Katawatin, Saksiri Sirisathien

The Thai Journal of Veterinary Medicine

This study was done to evaluate the efficiency of ultrasound-guided transvaginal follicular aspiration without hormonal pre-stimulation for collecting the oocytes of indigenous Thai beef cattle (Bos indicus), and to assess the vitrification method for their preservation. All animals between 2.5-12 years were categorized according to age (i.e. heifer, young cow, and old cow). A medium-sized follicle (φ 3-8 mm.) had the greatest distribution among the various size classes (p≤ 0.05), and the mean of oocytes collected from each group was not significantly different. Postthawed survival was 60.78, 53.70 and 57.50%, with a cleavage rate of 38.71, 37.93 and 39.13%, and …

In Field Fracture Management In Horses, Carstanjen Bianca, Siewruk Kasia, Gajewski Zdzislaw

The Thai Journal of Veterinary Medicine

Veterinary practitioners can be confronted with limb fractures in all types of horses. Immediate evaluation of the horse’s vital parameters, diagnosis, medication and stabilization of the fractured limb with splint bandages or fiberglass cast are of uppermost importance for a possible therapy. Adequate professional transportation of the horse with a fracture to a specialized referral center for diagnosis and therapy is crucial for a positive treatment strategy. This summary article describes in field emergency management of the equine patient with a fracture.

Effect Of Catalase And Superoxide Dismutase On Motility, Viability And Acrosomal Integrity Of Canine Spermatozoa During Storage At 5๐c, Khongsak Thiangtum, Tatsuya Hori, Eiichi Kawakami

The Thai Journal of Veterinary Medicine

Preservation of canine spermatozoa at 5๐C is a helpful method for dog breeding management. However, in many species, oxidative damage due to generation of reactive oxygen species (ROS) and long term storage which specifically cause DNA damage, and lipid peroxidation during preservation techniques can cause a decline of sperm motility and fertility capacity. In this study, the effect of antioxidants catalase (CAT) and superoxide dismutase (SOD) on motility, viability and acrosomal integrity of chilled canine semen were investigated. Semen was collected by digital manipulation from 4 dogs (two ejaculates/dog). After removal of seminal plasma, spermatozoa were diluted in egg yolk …

Structure-Function Analysis Of A Protein Encoded By The Bhv-1 Latency Related Gene, Devis Sinani

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Bovine herpes virus 1 (BHV-1) is a significant viral pathogen in cattle that induces a myriad of clinical symptoms. These symptoms include: conjunctivitis, upper respiratory tract infections, genital disorders, and abortions. BHV-1 infection can also lead to transient immune-suppression, which predisposes cattle to secondary bacterial infection leading to life-threatening pneumonia referred to as bovine respiratory disease (BRD). Following acute infection, BHV-1 establishes latency in sensory neurons within trigeminal ganglia. Reactivation of the virus can occur periodically, resulting in virus transmission. The latency-related (LR) RNA is the only abundantly expressed transcript in latently infected sensory neurons and it encodes several proteins, …

Socioeconomic Determinants Of Geographic Disparities In Campylobacteriosis Risk: A Comparison Of Global And Local Modeling Approaches, Jennifer Weisent, Barton Rohrbach, John Dunn, Agricola Odoi

Agricola Odoi

Background: Socioeconomic factors play a complex role in determining the risk of campylobacteriosis. Understanding the spatial interplay between these factors and disease risk can guide disease control programs. Historically, Poisson and negative binomial models have been used to investigate determinants of geographic disparities in risk. Spatial regression models, which allow modeling of spatial effects, have been used to improve these modeling efforts. Geographically weighted regression (GWR) takes this a step further by estimating local regression coefficients, thereby allowing estimations of associations that vary in space. These recent approaches increase our understanding of how geography influences the associations between determinants and …

Associations Of Passerine Birds, Rabbits, And Ticks With Borrelia Miyamotoi And Borrelia Andersonii In Michigan, U.S.A., Sarah A. Hamer, Graham J. Hickling, Rich Keith, Jennifer L. Sidge, Edward D. Walker, Jean I. Tsao

Animal Science Publications and Other Works

Background

Wild birds contribute to maintenance and dissemination of vectors and microbes, including those that impact human, domestic animal, and wildlife health. Here we elucidate roles of wild passerine birds, eastern cottontail rabbits (Sylvilagus floridanus), and Ixodes dentatus ticks in enzootic cycles of two spirochetes, Borrelia miyamotoi and B. andersonii in a region of Michigan where the zoonotic pathogen B. burgdorferi co-circulates.

Methods

Over a four-year period, wild birds (n = 19,631) and rabbits (n = 20) were inspected for tick presence and ear tissue was obtained from rabbits. Samples were tested for Borrelia spirochetes using nested PCR …

Subcongenic Analysis Of Tabw2 Obesity Qtl On Mouse Chromosome 6, Taryn P. Stewart, Xia Mao, Maha N. Aqqad, Dean Uffort, Kristy D. Dillon, Arnold M. Saxton, Jung H. Kim

Animal Science Publications and Other Works

Background

We previously established a congenic mouse strain with TALLYHO/Jng (TH) donor segment on chromosome 6 in a C57BL/6 (B6) background that harbors an obesity quantitative trait locus, tabw2. The B6.TH-tabw2 congenic mice developed increased adiposity that became exacerbated upon feeding a high fat-high sucrose (HFS) diet. To fine map the tabw2, in this study we generated and characterized subcongenic lines with smaller TH donor segments.

Results

We fixed four subcongenic lines, with maximum size of donor segment retained in the lines ranging from 10.8 – 92.5 Mb. For mapping, all the subcongenic mice, along with B6.TH- …

Discovery Fall 2012, Misty Bailey

Discovery Research Newsletter

No abstract provided.