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Articles 1 - 30 of 63
Full-Text Articles in Medicine and Health Sciences
Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu
Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu
School of Veterinary and Biomedical Sciences: Faculty Publications
Replication of porcine circovirus type 2 (PCV2), an important worldwide swine pathogen, has been demonstrated to be influenced by host genotype. Specifically, a missense DNA polymorphism (SYNGR2 p.Arg63Cys) within the SYNGR2 gene was demonstrated to contribute to variation in PCV2b viral load and subsequent immune response following infection. PCV2 is known to induce immunosuppression leading to an increase in susceptibility to subsequent infections with other viral pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV). In order to assess the role of SYNGR2 p.Arg63Cys in co-infections, pigs homozygous for the favorable SYNGR2 p.63Cys (N = 30) …
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …
A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber
A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber
USDA Wildlife Services: Staff Publications
Pigs (Sus scrofa) may be important surveillance targets for risk assessment and risk-based control planning against emerging zoonoses. Pigs have high contact rates with humans and other animals, transmit similar pathogens as humans including CoVs, and serve as reservoirs and intermediate hosts for notable human pandemics. Wild and domestic pigs both interface with humans and each other but have unique ecologies that demand different surveillance strategies. Three fundamental questions shape any surveillance program: where, when, and how can surveillance be conducted to optimize the surveillance objective? Using theory of mechanisms of zoonotic spillover and data on risk factors, …
Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk
Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk
The Thai Journal of Veterinary Medicine
True hermaphrodites are animals with both male and female gonads that have concurrently developed in the same individual. The incidence of true hermaphroditism is moderately higher in pigs than in other domestic animals. The aim of this study was to describe the genital organs of gilts culled due to anestrus with true hermaphrodites by gross and histological analysis. Three anestrus gilts were categorized as unilateral true hermaphrodites, having one ovotestis and one ovary, while three other gilts were bilateral true hermaphrodites with two ovotestes. The anomalous structures of the female genital organs, such as oviductal aplasia and hyperclitoris were found …
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
The Thai Journal of Veterinary Medicine
The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …
Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Electronic Theses and Dissertations
Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …
Polymorphisms Of Exon 10 In The Bpi Gene And Its Association Analysis With Some Reproductive Traits And Partial Immune Indexes In Meishan Pigs, Huan Qu, Zongyou Wei, Lan Ye, Shenglong Wu, Wenbin Bao
Polymorphisms Of Exon 10 In The Bpi Gene And Its Association Analysis With Some Reproductive Traits And Partial Immune Indexes In Meishan Pigs, Huan Qu, Zongyou Wei, Lan Ye, Shenglong Wu, Wenbin Bao
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to detect polymorphisms of BPI gene exon 10 by PCR-RFLP in Meishan pigs and their associations with some important cytokine levels (IL-1ß, IL-4, IL-6, IL-8, IL-10, TGF-1ß, IFN-γ, TNF-α) and reproductive traits (total number born (TNB), number born alive (NBA), birth litter weight (BLW), and weaning litter weight (WLW)), with the aim of identifying effective genetic markers for molecular breeding. The results revealed three genotypes, including AA, AB, and BB, with the trend of AA > BB > AB in reproductive traits. Pigs with the AA genotype were significantly higher than the AB genotype in TNB and IFN-γ …
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
This study aimed to determine plasmid profile of E. faecium and E. faecalis isolated from pigs, pig products and humans in Thailand-Laos border provinces. A total of 96 Enterococcus spp. including E. faecium (n=59) and E. faecalis (n=37) from pigs, pork and humans were included. All were determined for their antimicrobial susceptibilities and plasmid profile. Most isolates (96.9%) carried one to four plasmids with a molecular weight of 0.03-35 kb in E. faecium and 19-40 kb in E. faecalis. The 26 kb plasmid was most commonly detected. Variable plasmid profiles were defined, of which the most common pattern was, 23 …
Evaluation Of Berberine As An Alternative To Antibiotics In Nursery Pig Diets, Emily Scholtz
Evaluation Of Berberine As An Alternative To Antibiotics In Nursery Pig Diets, Emily Scholtz
Electronic Theses and Dissertations
Pig weaning process results in reduced growth performance and gut health of weaned pigs. Antibiotics can be added in diets for weaned pigs to improve growth performance and gut health, but their use is being discouraged because they can lead to development of antimicrobial resistant microorganisms. Thus, there is need for alternatives to antibiotics in diets for weaned pigs. The overall goal of this thesis research was to determine the effects of plant extracts (berberine, quercetin, and allyl isothiocyante [AITC]) as alternatives to antibiotics in weaned pig diets. The first objective of the research was to determine in vitro antimicrobial …
Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay
Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay
Turkish Journal of Veterinary & Animal Sciences
The thymus is a primary lymphoid organ that provides the microenvironment required for the development of T lymphocytes. Thymic epithelial cells (TECs) are among the most critical components in the thymic microenvironment supporting thymocyte selection and maturation. Apoptosis is primarily a physiologic process that plays a major role during the development of T lymphocytes. The first purpose of this study was to examine morphological changes in apoptotic thymocytes by transmission electron microscope in piglets. The second purpose was to reveal the morphological typology of TECs in piglets. Tissue samples of the thymus were taken from healthy Yorkshire race pigs of …
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev
Turkish Journal of Veterinary & Animal Sciences
The most prospective and actual trend in farm animal breeding is studying gene polymorphisms affecting the productive traits. The objective of this investigation was to study the effect of polymorphisms in intron 1 of the swine POU1F1 gene on number of days to 100 kg (days to 100 kg), length of body (LB), backfat thickness (BF), and number of piglets born alive (NBA). Research was conducted on purebred pigs of Landrace (n = 80) and Duroc (n = 100) and crossbred pigs (Landrace x Large White) (n = 192). Insertions/deletions in intron 1 of the POU1F1 gene were defined by …
Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk
Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Post-partum and lactation are the most complex periods in the swine production chain. Newborn pigs are highly vulnerable due to relatively low body weight at birth and physiological immaturity. Most of the management strategies performed in farrowing houses are oriented to ensure a proper level of colostrum intake by the piglets. Colostrum is essential as an energy source and to provide passive immunity to piglets. Different farrowing supervising protocols have been comprehensively investigated to reduce early mortality as well as to assist newborn piglets in obtaining an optimal amount of colostrum and milk. However, little is known of the benefits …
Characterization Of Porcine Adiponectin Gene (Adipoq) Polymorphismsand Their Association With Litter Size, Xiaodong Zhang, Ning Zhou, Jie Zhou, Ning Wang, Xiang Qin, Hui Dong, Yueyun Ding, Zongjun Yin
Characterization Of Porcine Adiponectin Gene (Adipoq) Polymorphismsand Their Association With Litter Size, Xiaodong Zhang, Ning Zhou, Jie Zhou, Ning Wang, Xiang Qin, Hui Dong, Yueyun Ding, Zongjun Yin
Turkish Journal of Veterinary & Animal Sciences
Litter size is one of the most important economic traits for pig production as it is directly related to production efficiency. To evaluate the effects of the adiponectin (ADIPOQ) gene on porcine litter size, we investigated ADIPOQ polymorphisms in three breeds, Wannan Black pigs, Berkshire pigs, and BW pigs (crossbred pigs produced from mating male Berkshire pigs and female Wannan Black pigs), using the PCR-SSCP method. Three SNPs (c. 178G > A, c. 1165A > G, and c. 1138G > A) were identified. In Wannan Black pigs, association analysis indicated that the c. 178G > A SNP genotype was significantly associated with an effect …
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
The Thai Journal of Veterinary Medicine
Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. In the current study, genetic identification of sapovirus (SaV) in suckling piglets was investigated in Hunan province, China between May 2013 and May 2014. Diarrheic fecal (n=300) samples collected from suckling piglets in Hunan province, China were evaluated using RT-PCR and then sequenced. Overall, 45 of the 300 (15%) samples were found to contain porcine SaV. In addition, phylogenetic analysis showed that all the porcine SaV isolates belonged to the porcine SaV genogroup III (GIII). The results of the present investigation have implications for the control of …
Association Of Mx1 Gene Polymorphism With Some Economic Traits In Meishan Pigs, Xue Mei Yin, Ying Liu, Wen Hua Dong, Qiao Hui Zhao, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao
Association Of Mx1 Gene Polymorphism With Some Economic Traits In Meishan Pigs, Xue Mei Yin, Ying Liu, Wen Hua Dong, Qiao Hui Zhao, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the association of polymorphisms in exon 14 of the Mx1 gene with significant economic traits in Meishan pigs, with the aim of identifying genetic markers of disease resistance. PCR-RFLP analysis of exon 14 of the Mx1 gene was conducted and associations with some cytokine levels (IFN-$\alpha$, IFN-$\gamma$, IL-2, IL-6, IL-10, and IL-12), early body weight (birth weight, 20-day weight, and 40-day weight), and reproductive performance within different parities (total number born, number born alive, number in live weaning litter, birth weight, and weaning weight) were analyzed. The results showed that there were AA, AB, …
Effects Of Probiotics, Oligosaccharides, And Berberine Combinationson Growth Performance Of Pigs, Guoge Fan, Juan Chang, Qingqiang Yin, Xiao Wang, Xiaowei Dang
Effects Of Probiotics, Oligosaccharides, And Berberine Combinationson Growth Performance Of Pigs, Guoge Fan, Juan Chang, Qingqiang Yin, Xiao Wang, Xiaowei Dang
Turkish Journal of Veterinary & Animal Sciences
In order to find alternative feed additives to replace antibiotics for safe animal production, combinations of probiotics, oligosaccharides, and berberine were used in this study. Ninety 60-day-old pigs were assigned to 9 groups, with 10 pigs for each group. Group 1 was the control, group 2 had antibiotics added, and groups 3-9 had different levels of combinations added. The experimental period was 60 days. The results indicated that average daily gain and feed conversion ratio had no significant differences among the 9 groups (P > 0.05); however, the diarrhea rates in the control group and the high probiotic groups with low …
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Backfat in pigs consists of water, collagen, and lipid. Apart from age, body weight, and number of estrus expression, backfat thickness is one of the significant parameters to consider when selecting female pigs into breeding herds since it dominates a number of reproductive performances, e.g. puberty attainment, total piglets born (TB), and farrowing rate. Besides, backfat is one of the significant sources of hormones related to puberty attainment, such as leptin, insulin-like growth factor-I (IGF-I), and progesterone (P4). Evaluation of backfat thickness is majorly performed by an A-mode ultrasonography at P2 position; it provides more accurate body condition than visual …
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
The Thai Journal of Veterinary Medicine
Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) causes multisystemic diseases called porcine circovirus associated disease (PCVAD) in pigs leading to a significant economic loss in the swine industry. The objective of this study was to determine the efficacy of a developed sub-unit PCV2b vaccine using a recombinant truncated capsid protein expressed in Escherichia coli in PCVAD-affected pig farms. Five-week-old conventional pigs were allocated into 2 groups: vaccinated (n = 55) and control (n = 55) groups placed in the same building. The vaccinated pigs were given two shots of a developed vaccine with 2-week interval on day 0 and 14. Serum samples …
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the association among serum insulin-like growth factor-I (IGF-I), backfatthickness, and age at first observed estrus in replacement gilts. In total, 80 replacement gilt, Landrace (L, n = 48) andYorkshire (Y, n = 32), were included. The gilts were determined for age at first observed estrus between 167 to 212days of age. They were categorized according to age at first observed estrus into ≤ 200 days (n = 41) and > 200 days (n= 36) groups. Backfat thickness, body weight, and serum IGF–I concentration were measured before mating. Age atfirst mating, interval from boar exposure to first observed …
Effect Of Oxytocin Treatment On The Reproductive Performance Of Sows After Artificial Insemination With Liquid Semen, Kamil Duzinski, Damian Knecht, Pawel Gajewczyk
Effect Of Oxytocin Treatment On The Reproductive Performance Of Sows After Artificial Insemination With Liquid Semen, Kamil Duzinski, Damian Knecht, Pawel Gajewczyk
Turkish Journal of Veterinary & Animal Sciences
This study was designed to evaluate the effect of oxytocin treatment at the time of insemination on fertility and prolificacy of sows during the summer season (July and September). The study included 107 Polish Large White × Polish Landrace sows divided into 3 groups: C, control (without oxytocin); I, experimental (5 IU oxytocin administrated intramuscularly per sow, 2 min before insemination); and II, experimental (5 IU of oxytocin added into a 100-mL seminal dose, at the time of insemination). Semen was collected from 11 boars: 2 Polish Large White, 5 Polish Landrace, and 4 Duroc × Pietrain. The highest farrowing …
General Reproductive Properties In Pigs, İrfan Tur
General Reproductive Properties In Pigs, İrfan Tur
Turkish Journal of Veterinary & Animal Sciences
Pig breeding is usually undertaken for fat and meat production, but different sectors (cosmetics industry, pharmaceutical industry, medical sector, etc.) can also use pigs for alternate purposes. Pork is evaluated based on the status of an amended law regarding butchers' meat in Turkey. There have been 25 pig farms registered in Turkey since 7 July 2006 in the framework of compliance with the European Union. On average, a sow has 2.3 farrowings per year, producing 9.5 to 12.5 piglets per litter. It is important that high proportions of selected replacement gilts reach puberty at an early age in order to …
Isolation Of Streptococcus Species From The Tonsils Of Slaughtered Pigs, Arzu Funda Bağcigi̇l, Serkan İki̇z, Kemal Meti̇ner, Naci̇ye Yakut Özgür, Seyyal Ak, Ahmet Ati̇la Ilgaz
Isolation Of Streptococcus Species From The Tonsils Of Slaughtered Pigs, Arzu Funda Bağcigi̇l, Serkan İki̇z, Kemal Meti̇ner, Naci̇ye Yakut Özgür, Seyyal Ak, Ahmet Ati̇la Ilgaz
Turkish Journal of Veterinary & Animal Sciences
The palatine tonsils of 220 pigs were examined for the occurrence of Streptococcus suis and other Streptococcus species. Samples were cultured onto Colombia agar plates with 5% defibrinated horse blood and selective antibiotic supplement and were incubated at 37 °C for 24-48 h aerobically. Presumptive colonies were identified using API20 Strep (bioMérieux). S. suis was not detected in any of the 220 palatine tonsils examined. S. porcinus was the most common streptococcus recovered and was isolated from 68 (31%) pigs. From the samples, S. agalactiae was isolated from 19 (8.6%), S. equi subsp. zooepidemicus from 10 (4.5%), S. uberis from …
Development Of Semi-Nested Pcr For Detection Of 16s Rrna Gene Of Mycoplasma Hyosynoviae, Pacharee Thongkamkoon, Watcharachai Narongsak, Metta Makhanon, Kobayashi Hideki, Koshi Yamamoto
Development Of Semi-Nested Pcr For Detection Of 16s Rrna Gene Of Mycoplasma Hyosynoviae, Pacharee Thongkamkoon, Watcharachai Narongsak, Metta Makhanon, Kobayashi Hideki, Koshi Yamamoto
The Thai Journal of Veterinary Medicine
In Thailand, some breeders in pig farms have had osteoarthritis lesions for which M. hyosynoviae might be one of the causative agents. Therefore, the semi-nested polymerase chain reaction assay was developed for detection of Mycoplasma hyosynoviae in organs of pigs in Thailand using three oligonucleotide primers specific to 16S rRNA gene of M. hyosynoviae. The detection limit of purified DNA was 10 femtogram per reaction and of the simulated lung sample was 103 CFU per gram of sample. Preliminary study for tonsil-carrier state of M. hyosynoviae in slaughtered pigs revealed the presence of M. hyosynoviae in 5 out of 10 …
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in …
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the influence of four different commercial semen extenders used during theholding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality.Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semenextender used during the holding time (A, B, C and D). The extended semen was cooled down and centrifuged. Thesperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The semen were loadedinto 0.5 ml straws and frozen. Thawing was achieved by immersing the straws in …
Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu
Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu
Turkish Journal of Veterinary & Animal Sciences
In order to determine the availability of fermented protein feedstuffs (FPFS)-such as cottonseed, blood, and feather meals—with Aspergillus oryzae, 2 phases of feeding experiments were adopted for 7 weeks. Sixty 58-day-old crossbred pigs were assigned to 6 groups, 10 pigs per group. Group 1 was the control; groups 2, 3, and 4 were supplemented with 6%-7%, 12%-14%, and 18%-21% FPFS, respectively, by replacing the same percentage of soybean meal (SBM); group 5 was supplemented with 12%-14% unfermented protein feedstuffs (UFPFS) by replacing the same percentage of SBM; group 6 was on the same base as group 3 with digestive energy …
Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Di̇dar Aydin, Zi̇hni̇ Mutlu, Esma Yildar
Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Di̇dar Aydin, Zi̇hni̇ Mutlu, Esma Yildar
Turkish Journal of Veterinary & Animal Sciences
Due to the fact that they produce less post-operative pain and shorter hospitalisation times, laparoscopic surgery procedures are more widely used than many traditional open surgical methods. However, the carbon dioxide gas used to visualise the surgical site during the procedure causes complications such as an increase in the blood CO_2 level and acidosis. In the present study, 12 male pigs were used. Sedation was achieved via intramuscular injection of xylazine at a dose of 2 mg/kg and anaesthesia induction was done via slow intravenous injection of ketamine HCl at a dose of 2 mg/kg. Following endotracheal intubation, general anaesthesia …
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
The Thai Journal of Veterinary Medicine
Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura
The Thai Journal of Veterinary Medicine
We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …