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Articles 3511 - 3540 of 9770

Full-Text Articles in Medicine and Health Sciences

Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep Jan 2017

Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …

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Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse Jan 2017

Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse

Chulalongkorn University Theses and Dissertations (Chula ETD)

Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is a highly contagious respiratory disease in chickens, causing significant economic losses in poultry industry worldwide. IBV has a high frequency of genetic mutation and recombination in its genome, particularly in the S1 gene, resulting in the emergence of several new IBV variants. This potentially affects the effectiveness of currently used IBV vaccine. Therefore, the continuous monitoring of the currently circulating IBV strains is essential. In this study, the genetic characteristic of IBV strains circulating in Thai chicken flocks during 2014-2016 was investigated by analysis of the complete S1 gene. A …

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Canine Emotions As Seen Through Human Social Cognition, Miiamaaria V. Kujala Jan 2017

Canine Emotions As Seen Through Human Social Cognition, Miiamaaria V. Kujala

Animal Sentience

It is not possible to demonstrate that dogs (Canis familiaris) feel emotions, but the same is true for all other species, including our own. The issue must therefore be approached indirectly, using premises similar to those used with humans. Recent methodological advances in canine research reveal what dogs experience and what they derive from the emotions perceptible in others. Dogs attend to social cues, they respond appropriately to the valence of human and dog facial expressions and vocalizations of emotion, and their limbic reward regions respond to the odor of their caretakers. They behave differently according to the …

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Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur Jan 2017

Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. …

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Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki Jan 2017

Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki

Masters Theses

Fish natural habitats are increasingly contaminated with various estrogenic compounds, including 17β estradiol (E2). This compound causes adverse effects on the reproductive system of male fish; however, the effects of E2 on other aspects of fish metabolism, morphology and histopathological changes in internal organs are not well known. The objective of this study is to evaluate the effects of E2 exposure on the basal and stressed metabolic rate, morphological changes in body shapes, and histological changes in the liver tissues of sunfish species. Fish were held individually in ten gallon tanks under two treatments of 40 and 80 ng/L and …

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Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee Jan 2017

Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee

UNL Faculty Course Portfolios

This Peer Review of Teaching Project portfolio focuses on the Animal Physiology II course which is required for first year veterinary medicine students. Weekly quizzes assess baseline knowledge and had been administered individually and in groups. I hypothesized the discontinuation of group quizzes would increase student effort when preparing for quizzes. Unit exams involve scenario-based questions and require students to apply information. I hypothesized the implementation of group exams would help under-performing students improve their ability to apply information they had learned. Exams were still taken individually prior to being taken in groups to encourage adequate preparation. Student impacts were …

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Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ Jan 2017

Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses …

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Design Of Probiotic Dry Fermented Sausage (Sucuk) Production Withmicroencapsulated And Free Cells Of Lactobacillus Rhamnosus, Emel Ünal, Zerri̇n Ergi̇nkaya, Süleyman Polat, Emi̇r Ayşe Özer Jan 2017

Design Of Probiotic Dry Fermented Sausage (Sucuk) Production Withmicroencapsulated And Free Cells Of Lactobacillus Rhamnosus, Emel Ünal, Zerri̇n Ergi̇nkaya, Süleyman Polat, Emi̇r Ayşe Özer

Turkish Journal of Veterinary & Animal Sciences

The present study was performed to design sucuk production with microencapsulated and free cells of Lactobacillus rhamnosus and to investigate the effect of this production method on probiotic viability and quality characteristics on the final product. L. rhamnosus was microencapsulated with an optimal coating material combination by using response surface methodology and subsequently used in sucuk production as a probiotic culture. Microencapsulation protected L. rhamnosus against gastric and other stress conditions in sucuk composition. Sucuk production with microencapsulated L. rhamnosus was found to be similar to traditional sucuk in terms of textural, physicochemical, and sensorial properties. Novel sucuk production was …

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Fascia Lata Flap To Repair Perineal Hernia In Dogs: A Preliminary Study, Simone Guérios, Juliana Monteiro, Daniella Matos Da Silva Jan 2017

Fascia Lata Flap To Repair Perineal Hernia In Dogs: A Preliminary Study, Simone Guérios, Juliana Monteiro, Daniella Matos Da Silva

Turkish Journal of Veterinary & Animal Sciences

Perineal hernia (PH) in dogs normally requires surgical reconstruction of the pelvic diaphragm. The use of fascia lata grafts has been reported and described in previous studies for the correction of PH after failure of the conventional herniorrhaphy. The purpose of this report is to describe a modified technique of fascia lata flap (FLP) for PH repair in dogs. Six client-owned male dogs with PH were included in the study. PH was repaired with a FLP obtained from the dog's ipsilateral thigh and sutured directly into the perineal defect. Caudal scrotal orchiectomy was performed in all dogs that were not …

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Efficacy Of Repeatedly Used Cidr Device In Cattle Reproduction: A Metaanalysisreview Of Progesterone Concentration And Conception Rate, Muhammad Furqan Asghar Chacher, Armağan Çolak, Armağan Hayirli Jan 2017

Efficacy Of Repeatedly Used Cidr Device In Cattle Reproduction: A Metaanalysisreview Of Progesterone Concentration And Conception Rate, Muhammad Furqan Asghar Chacher, Armağan Çolak, Armağan Hayirli

Turkish Journal of Veterinary & Animal Sciences

n order to reduce estrus synchronization cost, utilization of residual progesterone (P4) in the used controlled internal drug release (CIDR) device upon sanitization can be possible. Two datasets were compiled from 8 and 7 research articles involving 3434 (2653 heifers + 771 lactating cows + 10 ovariectomized cows) and 7301 cattle (3879 heifers + 3422 lactating cows) to evaluate blood P4 concentration and conception rate, respectively, in response to repeatedly used CIDR devices after sanitization via autoclaving and disinfection. As the number of CIDR usages increased, blood P4 concentration decreased linearly (y = -0.484X + 3.135, R2 = 0.99, P < 0.001). Blood P4 concentration (1.52 vs. 2.20 ng/mL; P < 0.002) and conception rate (40.76 vs. 32.96%, P < 0.05) for lactating cows were lower than those for heifers. The sanitization method did not affect blood P4 concentration, but reused CIDR device subjected to disinfection was associated with reduced conception rate. In summary, depending upon the initial P4 load (1.38-1.90 g), the CIDR device could be used twice in lactating cows and four times in heifers after autoclaving to achieve target blood P4 concentrations.

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Comparison Of The Isolation And Inhibition Abilities Ofselective Media Used For Brucella Spp. Isolation, Mustafa Sencer Karagül, Serkan İki̇z Jan 2017

Comparison Of The Isolation And Inhibition Abilities Ofselective Media Used For Brucella Spp. Isolation, Mustafa Sencer Karagül, Serkan İki̇z

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare and contrast the isolation ability of selective media developed for Brucella spp. isolation and their inhibition ability against contaminant microorganisms. Fifty-one field strains biotyped from abortion case samples and 25 Brucella spp. negative organ samples were used. Strain suspensions and organ suspensions were prepared separately. The turbidity of the strain suspensions was measured via spectrophotometer. The bacterial density of the strain suspensions was prepared in such a way that the suspensions would have different turbidity values. Inoculation into Farrell, Agrifood Research and Technology Center of Aragon, Jones & Morgan, and Modified Thayer …

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Development Of Multiplex Rt-Pcr For Detection And Differentiationof Foot-And-Mouth Disease Virus O And A Serotypes In Turkey, Beyhan Sareyyüpoğlu, İbrahi̇m Burgu Jan 2017

Development Of Multiplex Rt-Pcr For Detection And Differentiationof Foot-And-Mouth Disease Virus O And A Serotypes In Turkey, Beyhan Sareyyüpoğlu, İbrahi̇m Burgu

Turkish Journal of Veterinary & Animal Sciences

In this study, a two-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed and evaluated using designed primers for the simultaneous detection and differentiation of Turkish foot-and-mouth disease virus (FMDV) serotypes A and O from clinical samples. In the method, a mix of one universal reverse primer designed from the 2B gene and two serotype-specific forward primers designed from the hypervariable regions of the capsid VP1-1D coding gene of FMDV were used. Totally, 272 FMDV-infected samples collected between 2006 and 2008 constituted the material of the study. mRT-PCR was compared to ELISA in terms of positivity percentage (%) and the …

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The Csnp Scanning And Expression Analysis Of The Duck Fth1 Gene, Yu Zhang, Yiyu Tong, Yang Chen, Zhengyang Huang, Zhen Zhu, Yang Zhang, Qi Xu, Guohong Chen Jan 2017

The Csnp Scanning And Expression Analysis Of The Duck Fth1 Gene, Yu Zhang, Yiyu Tong, Yang Chen, Zhengyang Huang, Zhen Zhu, Yang Zhang, Qi Xu, Guohong Chen

Turkish Journal of Veterinary & Animal Sciences

Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one …

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A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi Jan 2017

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …

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Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut Jan 2017

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …

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Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska Jan 2017

Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska

Turkish Journal of Veterinary & Animal Sciences

Hematological effects of etomidate and tricaine at concentrations causing general anesthesia or sedation of carp juveniles were compared. Recovery of body balance took similar time in the case of both anesthetics, but fish treated with etomidate were depressed for several hours, while those subjected to tricaine immediately regained normal behavior. Immediately after exposure anesthetics caused minor hematological changes; the only statistically significant effects (decrease of hematocrit and plasma glucose) occurred after sedation with etomidate. One week after exposure a significant increase in mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration occurred in all groups of fish compared to the previous …

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Influence Of Dietary Vitamin D3 Supplementation On The Color Changes In Longissimus Lumborum, Vastus Lateralis, Semitendinosus, And Semimembranosus Beef Muscles, Urszula Karolina Rafalska Jan 2017

Influence Of Dietary Vitamin D3 Supplementation On The Color Changes In Longissimus Lumborum, Vastus Lateralis, Semitendinosus, And Semimembranosus Beef Muscles, Urszula Karolina Rafalska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of dietary vitamin D3 supplementation on changes in color and heme pigment (myoglobin and metmyoglobin) in 4 beef muscles. The muscles came from 48 crossbred bulls that were subjected to supplementation with vitamin D3 at 4 different doses: 0.0 (control group), 3.5, 7.0, and 10.0 x 106 IU. The 3.5 x 106 IU dose was administered daily for 6 days, before the morning feeding. Four days before slaughter, the supplementation was withdrawn. The dose of 7.0 and 10.0 x 106 IU was administered for 3 days and withdrawn 7 days …

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Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs, Murat Sarierler, Zeynep Bozkan Tatli, İbrahi̇m Akin, Rahi̇me Yaygingül, Onur Özgün Deri̇ncegöz Jan 2017

Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs, Murat Sarierler, Zeynep Bozkan Tatli, İbrahi̇m Akin, Rahi̇me Yaygingül, Onur Özgün Deri̇ncegöz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the normal ranges of proximal femoral and acetabular parameters in 26 adult healthy Sivas Kangal dogs (SKDs). Significant differences were observed between the sexes in hip axis length (HAL) of 8.03 ± 0.82 cm and 7.47 ± 0.40 cm, femoral neck axis length-a (FNALa) of 6.40 ± 0.55 cm and 6.09 ± 0.27 cm, FNALb of 5.09 ± 0.47 cm and 4.78 ± 0.31 cm, acetabular width (AW) of 1.62 ± 0.35 cm and 1.37 ± 0.24 cm, femoral shaft diameter (FSD) of 3.11 ± 0.31 cm and 2.88 ± 0.21 cm, …

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Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep Jan 2017

Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …

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Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr Jan 2017

Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …

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Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr Jan 2017

Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …

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Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul Jan 2017

Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, …

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The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak Jan 2017

The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Leaky gut characterized as decreased transepithelial electrical resistance and increased paracellular permeability to macromolecules is associated with several fatal diseases including sepsis. Pro-inflammatory cytokine IL-1β is a critical mediator of underlying mechanism through activation of myosin light chain kinase pathway. Blocking the inducible phosphorylated myosin light chain (p-MLC) indicates the successful treatment. Xanthones predominantly found in mangosteen (Garcinia mangostana) has been indicated as a potent anti-inflammatory action. Hydroxyxanthones (HDX), the major natural xanthones, have been recently synthesized in various forms. This study aimed to examine and screen the action of various forms of synthetic HDXs, in preventing the increased paracellular …

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Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak Jan 2017

Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The present study was performed to determine the differences of some seminal plasma proteins, i.e., glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), and some spermatozoa proteins, i.e., triosephosphate isomerase (TPI) and ACRBP (acrosin binding protein), in the boar semen having good and poor freezability. The study was conducted by including 38 ejaculates in seminal plasma study and 32 ejaculates from spermatozoa study from 27 boars (Duroc=13, Landrace= 8, Yorkshire= 6). The semen was split into two portions. The first portion was centrifuged to separate the pellet of sperm from the seminal plasma. The samples were kept at −80 °C …

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The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart Jan 2017

The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed at examining the effects of different concentrations of hydroxyethyl starch (HES) on quality of equine sperm following cold storage and cryopreservation. In experiment 1, the semen was collected from 6 stallions (3 replicates). The semen was diluted with extender for cold storage containing different concentrations of HES (2.5%, 5%, 10% and 15%). Extender without HES (0% HES) served as a control group. The temperature of extended semen was gradually decreased to 4 ºC. The quality of sperm in terms of progressive motility and viability was evaluated after cold storage for 6, 24 and 48 hours. This study …

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Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee Jan 2017

Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aims of the present study was to investigate factors associated with colostrum consumption of the neonatal piglets and determine the concentrations of total immunoglobulins G (IgG) and immunoglobulins A (IgA) against porcine epidemic diarrhea (PED) virus in colostrum for sow. The study consisted of two parts: Part I, the study included data of colostrum consumption from 1,140 neonatal piglets from 80 sows. Factors associated with piglet’s colostrum consumption included body weight at birth of the piglet, birth order, birth interval, heart rate, blood oxygen saturation, rectal temperature at 24 h, gestation length, total born, born alive, sow body conditions …

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Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan Jan 2017

Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rabbit is a promising model for studying myocardial infarction as several physiological and pathological conditions are resemble to humans. However, the information on establishment of rabbit pluripotent stem cells (PSC) and differentiation potential especially cardiac differentiation in this species has been limited. This research aimed at establishing and examining in vitro cardiogenesis of rabbit embryonic stem cells (ESC) and rabbit induced pluripotent stem cells (iPSC). In experiment 1, four cell lines of ESC derived from blastocyst stage embryos were cultured and characterized for their pluripotency in terms of gene and immunohistochemistry. They exhibited normal karyotypes (n=44). Embryoid body (EB) formation …

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Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun Jan 2017

Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycoplasma gallisepticum (MG) is the respiratory pathogen causing chronic respiratory disease (CRD) in poultry. More importantly, MG infection affects the economic losses of poultry industry due to decrease egg production and carcass quality. There are several procedures for MG monitoring and several commercial vaccines to prevent and control the MG infection. In Thailand, MG infection has been the one of major problems of poultry industry. The objectives of this study were to investigate the different molecular techniques for MG monitoring in the poultry farms and to prepare the inactivated vaccine or bacterin with Thai MG strain. There were 3 experiments …

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Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan Jan 2017

Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Comparative study was conducted to measure urinary N-acetyl-β-glucosaminidase (NAG), urinary glycosaminoglycans (GAGs) and plasma glycosaminoglycans (GAGs) in cats with feline idiopathic cystitis (FIC). A standard questionnaire was designed to gather information for all cats including signalment, characteristics, environment, type of food and management of the cats’ litter box to evaluate the risk factors for developing FIC. Blood and urine samples were collected from 19 clinically normal cats and 19 aged and sex matched cats with FIC. Concentration of urinary NAG, urinary GAGs and plasma GAGs were measured by colorimetric method. NAG index and GAGs-to-creatinine ratio were calculated. The results demonstrated …

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The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul Jan 2017

The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Study involved with potassium channel KCa3.1 blocker (triarylmethane-34; TRAM-34) in doxorubicin (DOX)-induced feline kidney cells was performed. The study was divided into two parts. In part I, subtoxic dose of TRAM-34 in feline kidney cell lines was studied to determine the cytotoxicity of TRAM-34 in feline kidney cells by cytotoxicity assay. In part II, feline kidney cell lines were incubated with appropriate dose and time of TRAM-34 pretreatment for 24 h or 30 min and post-treatment for 24 h condition in DOX-induced cell toxicity. Cytotoxicity assay, apoptosis and necrosis assay and KCa3.1 protein expression were measured. The cytotoxicity results indicated …

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