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Articles 3151 - 3180 of 9770
Full-Text Articles in Medicine and Health Sciences
Comparison Of Pre- And Postpartum Serum Leptin, Ghrelin, And Lipid Levels In Sheep, Ethem Mutlu Temi̇zel, Hüseyi̇n Ci̇han, Pinar Levent, Ahmet Saril, Yeşi̇m Özarda, Zeki̇ Yilmaz
Comparison Of Pre- And Postpartum Serum Leptin, Ghrelin, And Lipid Levels In Sheep, Ethem Mutlu Temi̇zel, Hüseyi̇n Ci̇han, Pinar Levent, Ahmet Saril, Yeşi̇m Özarda, Zeki̇ Yilmaz
Turkish Journal of Veterinary & Animal Sciences
Serum leptin and ghrelin play important roles in energy metabolism and inducing appetite during pregnancy and lactation. We investigated the serum levels of leptin and ghrelin and their relationship with the duration of lactation and serum biochemical responses during the prepartum (last week before lambing) and postpartum periods in sheep. They were also evaluated between pregnant and nonpregnant (control ewes) ewes. For these purposes, pregnant (n = 15) and control Awassi ewes (n = 15) that were 2 years of age were used in this study. Blood samples were collected 1 week before the expected date of lambing (baseline) and …
The Pathology Of Tuberculosis Lesions In Allergic Skin Test (Ppd Tuberculin) Positive Cows And Detection Of Tuberculosis Agents With Pcr In Milk Samples, Çi̇ğdem Tuncay, Fati̇h Hati̇poğlu
The Pathology Of Tuberculosis Lesions In Allergic Skin Test (Ppd Tuberculin) Positive Cows And Detection Of Tuberculosis Agents With Pcr In Milk Samples, Çi̇ğdem Tuncay, Fati̇h Hati̇poğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine tuberculosis (Tb) agents in milk and the pathologic changes in the tissues and organs of 100 PPD Tb-positive cows that were intended for slaughter. Milk samples were taken before slaughtering and PCR analysis was conducted to detect the Tb agent. In postmortem examinations, the lungs, liver, mammary glands, spleen, kidneys, and heart were collected for macroscopic and histopathological examinations. Out of the 100 PPD positive cows, lesions of Tb were observed in 75 cows. In the microscopic examination, Tb was identified in the lungs (51 cases), liver (61 cases), and in both …
The Effect Of Caffeine On Neuron Number Of Rats Exposedto 900-Mhz Electromagnetic Field, Aydin Him, Nimet Burcu Deniz, Mehmet Emi̇n Önger
The Effect Of Caffeine On Neuron Number Of Rats Exposedto 900-Mhz Electromagnetic Field, Aydin Him, Nimet Burcu Deniz, Mehmet Emi̇n Önger
Turkish Journal of Veterinary & Animal Sciences
Previous studies have shown that the electromagnetic field (EMF) emitted by cell phones decreased the number of neurons in some parts of the brain. It was also shown that caffeine protects the nerve cells. This study examines if caffeine has any effects on preventing neuron loss in rats exposed to EMF. Rats were exposed to a 900-MHz EMF for 60 min a day for 28 days. The rats that were exposed to EMF were fed 1 mg/L caffeinated water instead of tap water. The rats in the sham group were put inside the exposure chamber but were not exposed to …
The Effects Of Three Different Prokinetic Drugs On The Motility Hormones Level In Lambs With Postoperative Ileus, Semi̇h Altan, Kaan Dönmez, Feray Altan, Fahretti̇n Alkan
The Effects Of Three Different Prokinetic Drugs On The Motility Hormones Level In Lambs With Postoperative Ileus, Semi̇h Altan, Kaan Dönmez, Feray Altan, Fahretti̇n Alkan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the effects of the three different prokinetic agents, namely erythromycin, lidocaine, and metoclopramide in lambs with postoperative ileus (POI) using motilin and ghrelin, which are known as motility markers. Twenty lambs were allocated to 4 groups of 5 lambs each. An enterotomy was performed on the ileum of each lamb. Erythromycin (8.8 mg/kg), metoclopramide (0.2 mg/kg), and saline (2 mL) were administered as single dose IM before surgery in the ERT, MET, and CNTR groups, respectively. Lidocaine was administered to the LID group, both as a bolus before (1.3 mg/kg IV) and …
Comparative Study Of Growth Patterns In Seven Strains Of Japanese Quail Using Nonlinear Regression Modeling, Hadi Farajiarough, Mohammad Rokouei, Ali Maghsoudi, Mahmoud Ghazaghi
Comparative Study Of Growth Patterns In Seven Strains Of Japanese Quail Using Nonlinear Regression Modeling, Hadi Farajiarough, Mohammad Rokouei, Ali Maghsoudi, Mahmoud Ghazaghi
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare the growth pattern of seven strains of Japanese quail including Wild, Italian Speckled, Tuxedo, Scarlett, English white, White button, and A&M Texas by nonlinear growth functions including Gompertz, Richards, logistic, Lopez, and Weibull functions. The body weights of the unselected randomly bred populations of these strains were measured weekly from 1 to 42 days of age and then used to fit functions. The fitness of each function was assessed by statistics including the mean square error, Akaike information criterion, Bayesian information criterion, and adjusted coefficient of determination. The results showed that Gompertz …
Severe Granulomatous Hepatitis Caused By Capillaria Hepatica In A Puppy, Ayli̇n Alasonyalilar Demi̇rer, Ahmet Akkoç, Bayram Şenli̇k, İbrahi̇m Taci̇ Cangül
Severe Granulomatous Hepatitis Caused By Capillaria Hepatica In A Puppy, Ayli̇n Alasonyalilar Demi̇rer, Ahmet Akkoç, Bayram Şenli̇k, İbrahi̇m Taci̇ Cangül
Turkish Journal of Veterinary & Animal Sciences
This report describes the inflammation caused by Capillaria hepatica in the liver of a female stray dog of approximately 2 months old for the first time in Turkey. Histopathological examination revealed severe granulomatous hepatitis with occasional adult nematodes and abundant eggs in the hepatic parenchyma. The present case differs from the other capillariasis cases by the age of the dog and the presence of mature female parasites and severe inflammation.
Alterations Of Circulating Cortisol, Igf-1, And Thyroid Hormones In Darehshori Horses During A Year, Aliasghar Chalmeh, Mehrdad Pourjafar, Khalil Badiei, Mohammad Mazrouei Sebdani, Ali Sadeghpour, Hasan Sheikhi, Ali Khezri
Alterations Of Circulating Cortisol, Igf-1, And Thyroid Hormones In Darehshori Horses During A Year, Aliasghar Chalmeh, Mehrdad Pourjafar, Khalil Badiei, Mohammad Mazrouei Sebdani, Ali Sadeghpour, Hasan Sheikhi, Ali Khezri
Turkish Journal of Veterinary & Animal Sciences
Season may be considered as a factor that affects the equine endocrine profile. Hence, from January to November 2015, 27 clinically healthy adult Darehshori racehorses were selected. Thirteen of them were stallions and the others were mares. Ten of them were more than 10 years old and 17 horses were under the age of 10 years. None of them were pregnant or lactating during the study. Body condition scores of 12 horses were below and the others were above 5. Blood samplings and body condition score evaluations were performed every 45 days during a year. Serum levels of cortisol, IGF-1, …
Determination Of Methicillin Resistance And Some Genotypic Characteristics Ofstaphylococci Isolated From Dogs And Their Owners, Arzu Findik, Alper Çi̇ftci̇, Taylan Önyay, Merve Gi̇zem Sezener, Yağmur Koçak, Ti̇mur Gülhan
Determination Of Methicillin Resistance And Some Genotypic Characteristics Ofstaphylococci Isolated From Dogs And Their Owners, Arzu Findik, Alper Çi̇ftci̇, Taylan Önyay, Merve Gi̇zem Sezener, Yağmur Koçak, Ti̇mur Gülhan
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the methicillin resistance and some genotypic characteristics of staphylococci in dogs and their owners. A total of 132 swab samples from 33 healthy dogs and their owners were taken. Isolated staphylococci were identified by PCR. The antibiotic sensitivities of the isolates were determined by disc diffusion method. Determination of pvl, mecA, bla, and fem genes and SCCmec types was performed by PCR. Isolates were genotyped according to coa and spa gene polymorphisms by PCR. Fortyfive isolates were identified as Stapyloccoccus spp. Among them, 8 isolates were identified as S. aureus and 23 isolates were identified …
Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena
Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena
Turkish Journal of Veterinary & Animal Sciences
The present study was planned to detect the genetic elements of Mannheimia haemolytica and Pasteurella multocida in pneumonic sheep lungs. Pneumonia was diagnosed on the basis of gross pathological lesions. Lung tissues were collected at necropsy of sheep (n = 96) and subjected to isolation of total DNA. The M. haemolytica-specific PHSSA and Rpt2 genes, and the P. multocidaspecific KMT1 and the Omp87 genes were amplified using polymerase chain reaction (PCR). A housekeeping gene targeting the sheep cellular mitochondrial 12S ribosomal DNA was used as an internal control. PCR reactions were optimized using the positive and negative controls. Gene-specific PCR …
Investigation Of The Efficiency And Safety Of Tilmicosin Phosphate In Treating Experimentalmycoplasmal Infections In Pigs, Xiaohui Zhang, Jinzhe Pan, Ning Wu, Shu Tang, Xiangdong Lei, Yangyang Sun, Joerg Hartung, Endong Bao
Investigation Of The Efficiency And Safety Of Tilmicosin Phosphate In Treating Experimentalmycoplasmal Infections In Pigs, Xiaohui Zhang, Jinzhe Pan, Ning Wu, Shu Tang, Xiangdong Lei, Yangyang Sun, Joerg Hartung, Endong Bao
Turkish Journal of Veterinary & Animal Sciences
In order to evaluate the in vivo therapeutic effect of oral administration of tilmicosin phosphate on mycoplasmal pneumonia in swine and its safety for the infected swine, the model of mycoplasmal pneumonia disease in swine was established by artificial infection, and indices such as efficiency, cure rate, death rate, the score of lung lesion, and the routine blood tests, blood biochemistry, and routine urine tests of tested swine were determined. The results showed that all of the dosage groups of 100 mg/L, 80 mg/L, 60 mg/L of 10% tilmicosin phosphate soluble powder demonstrated distinct therapeutic effects on mycoplasmal pneumonia in …
Effects Of The Supplementation Of Lamb Rations With Oregano Essential Oil On Theantimicrobial And Antioxidant Metabolism In Meat, Hali̇t İmi̇k, Sevda Urçar, Meryem Aydemi̇r Atasever, Recep Gümüş, Hayrunni̇sa Özlü
Effects Of The Supplementation Of Lamb Rations With Oregano Essential Oil On Theantimicrobial And Antioxidant Metabolism In Meat, Hali̇t İmi̇k, Sevda Urçar, Meryem Aydemi̇r Atasever, Recep Gümüş, Hayrunni̇sa Özlü
Turkish Journal of Veterinary & Animal Sciences
Although oregano essential oil has common use in the cosmetics, pharmaceutical, and food sectors, information on its effects on meat quality and shelf life, when incorporated into lamb feed rations, is scarce. In this study, the effects of oregano essential oil (OEO) in feed on the water activity, pH, lipid peroxidation, color parameters, and microbial counts of the Musculus longissimus dorsi (MLD) were investigated. The control group was fed a basal ration and the OEO1 and OEO2 groups were fed a basal ration supplemented with 200 mg/kg and 400 mg/kg OEO, respectively. Akkaraman lambs were fed experimental rations for a …
A Stereological Study On Calculation Of Volume Values Of The Cervical Spinalsegments In Ducks, Gamze Çakmak, Hüseyi̇n Karadağ, Zafer Soygüder, Murat Çeti̇n Rağbetli̇, Serkan Yildirim, Haci Keleş
A Stereological Study On Calculation Of Volume Values Of The Cervical Spinalsegments In Ducks, Gamze Çakmak, Hüseyi̇n Karadağ, Zafer Soygüder, Murat Çeti̇n Rağbetli̇, Serkan Yildirim, Haci Keleş
Turkish Journal of Veterinary & Animal Sciences
In the present study, volume values of the cervical segments in the spinal cords of adult ducks weighing 3-4 kg were investigated. Volume values of the grey matter and white matter in the cervical segment of the spinal cord of the ducks were examined stereologically. Ten ducks (genus Anas) without regarding the sex of the animals. All animals were perfused using 10% formaldehyde. The animals were kept in 10% formaldehyde for 1 week to ensure fixation. The ducks were then dissected. The cervical segments were uncovered by removing the cervical vertebrae. Tissue samples were obtained from each of the cervical …
Chronic Diarrhea Due To Lymphosarcoma In An Adult Cow: A Sporadic Clinical Report, Nuzhat Hassan, Charanjit Singh Randhawa, Swaran Singh Randhawa, Deepti Narang
Chronic Diarrhea Due To Lymphosarcoma In An Adult Cow: A Sporadic Clinical Report, Nuzhat Hassan, Charanjit Singh Randhawa, Swaran Singh Randhawa, Deepti Narang
Turkish Journal of Veterinary & Animal Sciences
The present clinical report describes a rare sporadic clinical case of chronic diarrhea due to lymphosarcoma in a 7-yearold crossbred Holstein?Friesian cattle. Diarrhea occurred due to intestinal lymphoid tissue involvement. The clinical diagnosis of lymphosarcoma was derived from peritoneal fluid aspirate, lymph node enlargement, and absence of serological and fecal evidence of any parasitic or bacterial infection. Reactive proliferative mesothelial hyperplasia in peritoneal fluid examination confirmed lymphosarcoma. The cells form numerous 3-dimensional clusters in mesothelial lymphosarcoma. Postmortem examinations confirmed visceral lymph node enlargements. A hematological examination revealed leukocytosis and a marked decrease in hemoglobin level, which reflected a severe degree …
Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai
Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The utilization of canine mesenchymal stem cells (cMSCs) with multipotent capabilities has been regarded for possible therapy of incorrigible bone disease. Although various sources of cMSCs show similar characteristics, they are different in osteogenic potential due to their original cellular sources. This study was designed to globally explore and analyze the in vitro differentiation potential and behavior of canine bone-marrow derived mesenchymal stem cells (cBM-MSCs) and canine dental pulp stem cells (cDPSCs) toward osteogenic lineage. An in vitro osteogenic differentiation potential of the cells was preliminarily compared in terms of alkaline phosphatase activity assay and Von Kossa staining. Global study …
Long-Term Outcome And Complications Of 32 Distal Femoral Osteotomies Performed For Correction Of Mpl In Dogs Without Cranial Cruciate Disease, Nicholas Crawfis
Long-Term Outcome And Complications Of 32 Distal Femoral Osteotomies Performed For Correction Of Mpl In Dogs Without Cranial Cruciate Disease, Nicholas Crawfis
Williams Honors College, Honors Research Projects
Many canines suffer severe, grade I-IV, medial patella luxation causing a persistent lameness, severely reducing the quality of life for the dog (Linney et al 2011). For these patients surgical intervention is recommended to alleviate these symptoms. The procedure used to do so is a distal femoral osteotomy (DFO). This procedure allows for correction of torsion in the femur and quadriceps mechanism, and frontal plane abnormalities at the same time. The present study aims to describe the long-term outcome and related complications of 32 DFOs, without concomitant Cranial Cruciate Ligament rupture (CCLR), in a large private practice referral hospital based …
Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford
Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford
United States Department of Agriculture Wildlife Services: Staff Publications
Anticoagulant rodenticides (ARs) are used to control rodents around homes, buildings, and in agriculture. They have been found widely in predatory and scavenging wildlife as a result of secondary exposure and less commonly in herbivores and omnivores from primary exposure. While predators and scavengers have been monitored for AR exposure, very little information is available about AR residues in edible muscle tissue of game animals. Game animals may be exposed to ARs through direct consumption of bait, ingestion of contaminated food or vegetation, or consumption of contaminated prey items. Carcasses of three species of game animals (black bear, wild pigs, …
Comparison Between The Anaesthetic Effects Of Xylazine?Ketamine And Diazepam?Ketamine: Physiological And Blood Parameters In Young Hamadryas Baboons (Papio Hamadryas), Fahd Alsobayil, Madeh Sadan, Abd Al-Azeez Meshaal Al-Shoshan
Comparison Between The Anaesthetic Effects Of Xylazine?Ketamine And Diazepam?Ketamine: Physiological And Blood Parameters In Young Hamadryas Baboons (Papio Hamadryas), Fahd Alsobayil, Madeh Sadan, Abd Al-Azeez Meshaal Al-Shoshan
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to evaluate the anaesthetic effects on young hamadryas baboons (Papio hamadryas) of xylazine-ketamine (XK) compared to diazepam-ketamine (DK). Six healthy young male hamadryas baboons were first premedicated with xylazine HCl (0.5 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). After a 10-day washout period, the hamadryas baboons were premedicated with diazepam (1 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). The onset, duration, and the depth of anaesthesia were determined by recording palpebral, corneal, and jaw reflexes. The results showed a significant decrease in heart rate …
Preference Of Side And Standing In Relationship With Milking Characteristics And Temperament Score Of Crossbred Dairy Cows In An 8 × 2 Herringbone Milking Parlour, Ahmad Fahim, Madan Lal Kamboj, Mukesh Bhakat, Tushar Kumar Mohanty, Rohit Gupta
Preference Of Side And Standing In Relationship With Milking Characteristics And Temperament Score Of Crossbred Dairy Cows In An 8 × 2 Herringbone Milking Parlour, Ahmad Fahim, Madan Lal Kamboj, Mukesh Bhakat, Tushar Kumar Mohanty, Rohit Gupta
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the preference of side and standing in relationship with milking characteristics of crossbred cows in a double-sided herringbone milking parlour. Seventy-two crossbred cows were milked in an 8 × 2 low-line automated herringbone milking parlour having automatic cluster removal settings for 45 milking sessions. There was considerable variation among individuals in the consistency of side choice (χ$^{2}$ = 1.414, P = 0.234). The consistency score of crossbred cows showed that many cows had a strong tendency to enter the parlour on the same side during consecutive milkings. The side preference was found …
Effect Of Ractopamine Hydrochloride On Growth Promotion In Guinea Pigs (Cavia Porcellus), Irene Citlalli Zuñiga Galindo, Jaime Alonso Navarro Hernandez, Shalaiko Christian Carlin Valderrabano, Julio Cesar Sanchez Lemus, Ivan Juarez Rodriguez, Dinorah Vargas Estrada
Effect Of Ractopamine Hydrochloride On Growth Promotion In Guinea Pigs (Cavia Porcellus), Irene Citlalli Zuñiga Galindo, Jaime Alonso Navarro Hernandez, Shalaiko Christian Carlin Valderrabano, Julio Cesar Sanchez Lemus, Ivan Juarez Rodriguez, Dinorah Vargas Estrada
Turkish Journal of Veterinary & Animal Sciences
Ractopamine hydrochloride (R) is commonly used in the livestock industry, mainly in pork and beef, to improve feed efficiency and productivity. In the present study, the use of R at different doses (20 ppm, 30 ppm, or 40 ppm) on growth performance and carcass characteristics of guinea pigs (male and female) was explored with a view to achieving greater use of this species for human consumption. Thirty-two clinically healthy guinea pigs at an average age of 5 weeks were used. The daily gain, feed over gain ratio, Kleiber ratio, carcass yield, and muscle thickness of the thigh in millimeters were …
Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Turkish Journal of Veterinary & Animal Sciences
Pestiviruses, which are distributed worldwide, may cause losses in sheep and goat populations. The current seroprevalence and circulation of pestiviruses in small ruminants of the South Marmara Region of Turkey were investigated. A total of 607 blood samples were collected from 22 different flocks in 4 different provinces, and 24 necropsy materials were investigated. Pestivirus seroprevalence in sheep and goats was determined by virus neutralization test against the NADL strain of bovine viral diarrhea virus. The mean antibody titer was 3.5, while the overall seropositivity rate was 32.78% (199/607). Seronegativity was markedly more prevalent in goats. The mean detected antibody …
Effects Of The Addition Of Essential Oils Cumimaldehyde, Eugenol, And Thymol On The In Vitro Gas Production And Digestibility Of Alfalfa (Medicago Sativa L.) Silage, Mustafa Salman, Habi̇p Muruz, Nurcan Çeti̇nkaya, Zehra Selçuk, İsmai̇l Kaya
Effects Of The Addition Of Essential Oils Cumimaldehyde, Eugenol, And Thymol On The In Vitro Gas Production And Digestibility Of Alfalfa (Medicago Sativa L.) Silage, Mustafa Salman, Habi̇p Muruz, Nurcan Çeti̇nkaya, Zehra Selçuk, İsmai̇l Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the in vitro effects of the addition of three essential oils, mainly cuminaldehyde, eugenol, and thymol to alfalfa (Medicago sativa L.) silage on gas production, digestibility, and metabolic energy. The essential oils were added at 0 ppm (controls) and 100 ppm (group 1), 200 ppm (group 2), and 300 ppm (group 3) with three replications. The cumulative amount of gas resulting from the addition of the three essential oils at the 96th h of incubation was significantly lower than that of the control group (P < 0.05). The cumulative amount of gas production at the 96th h of incubation with thymol ranged from 184.63 mL/g to 217.38 mL/g. In conclusion, this study showed that the addition of different amounts of cuminaldehyde, eugenol, and thymol to alfalfa silage reduced the amount of in vitro gas production. Therefore, these treatments can potentially reduce environmental pollution from ruminal digestion. However, the use of increasing levels of essential oils reduced the amounts of digestible organic matter and metabolic energy. Additional studies are necessary to reveal the effects of these essential oils on in vivo ruminal fermentation by measuring changes in feed consumption and productivity.
The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis
The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis
Turkish Journal of Veterinary & Animal Sciences
The present study was conducted to investigate the effects of three biotechnological products of Saccharomyces cerevisiae (organic selenium, concentrated mannan of S. cerevisiae, and living cells of the same yeast) used in broiler diet on growth performance, health status, and meat quality and composition. One hundred day-old broiler chickens were divided into four groups (control and three experimental groups using the above-mentioned diet supplements) and fed basic feed mixture (developed by the Nutrition Department at the University of Agricultural Sciences and Veterinary Medicine in Cluj-Napoca, Romania) for 42 days. After this period, from each group, five birds were sacrificed and …
The Combination Of Casa Kinetic Parameters And Fluorescein Staining As A Fertility Tool In Cryopreserved Bull Semen, Muhammed Enes İnanç, Beste Çi̇l, Koray Teki̇n, Havva Alemdar, Ali̇ Daşkin
The Combination Of Casa Kinetic Parameters And Fluorescein Staining As A Fertility Tool In Cryopreserved Bull Semen, Muhammed Enes İnanç, Beste Çi̇l, Koray Teki̇n, Havva Alemdar, Ali̇ Daşkin
Turkish Journal of Veterinary & Animal Sciences
Prediction of male fertility with in vitro semen quality parameters remains a challenge for the bull industry. Fluorescein staining and computer-assisted semen analysis (CASA) ensure kinetically proper and functionally objective results to improve spermatological parameter control. Therefore, we aimed to examine the CASA kinetic parameters and fluorescein staining of cryopreserved bull semen and its relationship with fertility. A total of 90 frozen straws from 5 Simmental bulls were evaluated. After semen was thawed, the motility, kinetic parameters, and insemination doses were evaluated by CASA; fluorescein stainings were evaluated using a fluorescein microscope. The 60th-day nonreturn rates (NRRs) were recorded for …
Development Of Septic Polysynovitis And Uveitis In Foals Experimentally Infected With Rhodococcus Equi, Laura Huber, Steeve Giguère, Londa J. Berghaus, Amanda Hanafi, Sarah Vitosh-Sillman, Sarah L. Czerwinski
Development Of Septic Polysynovitis And Uveitis In Foals Experimentally Infected With Rhodococcus Equi, Laura Huber, Steeve Giguère, Londa J. Berghaus, Amanda Hanafi, Sarah Vitosh-Sillman, Sarah L. Czerwinski
School of Veterinary and Biomedical Sciences: Faculty Publications
Rhodococcus equi is one of the most important causes of disease in foals. Infection is typically characterized by pyogranulomatous pneumonia although extrapulmonary infections occur occasionally. Uveitis and polysynovitis have been reported in foals naturally infected with R. equi and are thought to be the result of an immune-mediated process. However, the pathogenesis of these conditions is poorly understood. The objectives of this study were to document the occurrence of uveitis and polysynovitis after experimental infection with R. equi and to determine if these disorders are the direct result of infection at these sites. Foals between 3 and 4 weeks of …
Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt
Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt
School of Veterinary and Biomedical Sciences: Faculty Publications
Hydrated lime manure treatment was evaluated to determine porcine epidemic diarrhea virus (PEDV) susceptibility to alkaline stabilization. At pH 10, PEDV decreased (quantitative polymerase chain reaction) and lost infectivity (swine bioassay). Although ammonium decreased above pH 9 (up to 25%), alkaline stabilization managed to control potential infection from manure sources.
Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry Identification Of Moraxella Bovoculi And Moraxella Bovis Isolates From Cattle, Kara Robbins, Aaron M. Dickey, Michael L. Clawson, John Dustin Loy
Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry Identification Of Moraxella Bovoculi And Moraxella Bovis Isolates From Cattle, Kara Robbins, Aaron M. Dickey, Michael L. Clawson, John Dustin Loy
School of Veterinary and Biomedical Sciences: Faculty Publications
Infectious bovine keratoconjunctivitis (IBK) is an economically significant disease caused by Moraxella bovis. Moraxella bovoculi, although not reported to cause IBK, has been isolated from the eyes of cattle diagnosed with IBK. Identification of M. bovis and M. bovoculi can be performed using biochemical or DNA-based approaches, both of which may be time consuming and inconsistent between laboratories. We conducted a comparative evaluation of M. bovoculi and M. bovis identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a database provided by Bruker Daltonics (termed the BDAL database), the BDAL database supplemented with spectra generated in …
Circadian Behavioral Responses To Light And Optic Chiasm-Evoked Glutamatergic Epscs In The Suprachiasmatic Nucleus Of Iprgc Conditional Vglut2 Knock-Out Mice, Michael G. Moldavan, Patricia J. Sollars, Michael R. Lasarev, Charles N. Allen, Gary E. Pickard
Circadian Behavioral Responses To Light And Optic Chiasm-Evoked Glutamatergic Epscs In The Suprachiasmatic Nucleus Of Iprgc Conditional Vglut2 Knock-Out Mice, Michael G. Moldavan, Patricia J. Sollars, Michael R. Lasarev, Charles N. Allen, Gary E. Pickard
School of Veterinary and Biomedical Sciences: Faculty Publications
Intrinsically photosensitive retinal ganglion cells (ipRGCs) innervate the hypothalamic suprachiasmatic nucleus (SCN), a circadian oscillator that functions as a biological clock. ipRGCs use vesicular glutamate transporter 2 (vGlut2) to package glutamate into synaptic vesicles and light-evoked resetting of the SCN circadian clock is widely attributed to ipRGC glutamatergic neurotransmission. Pituitary adenylate cyclase-activating polypeptide (PACAP) is also packaged into vesicles in ipRGCs and PACAP may be coreleased with glutamate in the SCN. vGlut2 has been conditionally deleted in ipRGCs in mice [conditional knock-outs (cKOs)] and their aberrant photoentrainment and residual attenuated light responses have been ascribed to ipRGC PACAP release. However, …
Development Of A Multiplex Real-Time Pcr Assay Using Two Thermocycling Platforms For Detection Of Major Bacterial Pathogens Associated With Bovine Respiratory Disease Complex From Clinical Samples, John Dustin Loy, Laura Leger, Aspen M. Workman, Michael L. Clawson, Ece Bulut, Bing Wang
Development Of A Multiplex Real-Time Pcr Assay Using Two Thermocycling Platforms For Detection Of Major Bacterial Pathogens Associated With Bovine Respiratory Disease Complex From Clinical Samples, John Dustin Loy, Laura Leger, Aspen M. Workman, Michael L. Clawson, Ece Bulut, Bing Wang
School of Veterinary and Biomedical Sciences: Faculty Publications
Bovine respiratory disease complex (BRDC) is one of the most significant diseases of cattle. Bacterial pathogens involved in BRDC include Mannheimia haemolytica, Mycoplasma bovis, Histophilus somni, and Pasteurella multocida. We developed and evaluated a multiplexed real-time hydrolysis probe (rtPCR) assay using block-based Peltier and rotary-based thermocycling on lung tissue, nasal swabs, and deep nasopharyngeal swabs. The rtPCR results were compared to culture or a gel-based M. bovis PCR using statistical analysis to determine optimum quantification cycle (Cq) cutoffs to maximize agreement. The limits of detection were 1.2–12 CFU/reaction for each pathogen. M. haemolytica was the most prevalent organism detected …
Coordinated Regulation Of Transcription By Ccpa And The Staphylococcus Aureus Twocomponent System Hptrs, Joseph M. Reed, Sean Olson, Danielle F. Brees, Caitlin E. Griffin, Ryan A. Grove, Paul J. Davis, Stephen D. Kachman, Jiri Adamec, Greg A. Somerville
Coordinated Regulation Of Transcription By Ccpa And The Staphylococcus Aureus Twocomponent System Hptrs, Joseph M. Reed, Sean Olson, Danielle F. Brees, Caitlin E. Griffin, Ryan A. Grove, Paul J. Davis, Stephen D. Kachman, Jiri Adamec, Greg A. Somerville
School of Veterinary and Biomedical Sciences: Faculty Publications
The success of Staphylococcus aureus as a pathogen is due in part to its ability to adapt to changing environmental conditions using signal transduction pathways, such as metaboliteresponsive regulators and two-component systems. S. aureus has a two-component system encoded by the gene pair sav0224 (hptS) and sav0223 (hptR) that regulate the hexose phosphate transport (uhpT) system in response to extracellular glucose-6-phosphate. Glycolytic intermediates such as glucose-6-phosphate are important carbon sources that also modulate the activity of the global metabolite-responsive transcriptional regulator CcpA. Because uhpT has a putative CcpA binding site in its promoter and …
Novel Docosahexaenoic Acid Ester Of Phloridzin Inhibits Proliferation And Triggers Apoptosis In An In Vitro Model Of Skin Cancer, Theodora Mantso, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, H. P. Vasantha Rupasinghe, Mihalis I. Panayiotidis
Novel Docosahexaenoic Acid Ester Of Phloridzin Inhibits Proliferation And Triggers Apoptosis In An In Vitro Model Of Skin Cancer, Theodora Mantso, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, H. P. Vasantha Rupasinghe, Mihalis I. Panayiotidis
School of Veterinary and Biomedical Sciences: Faculty Publications
Skin cancer is among the most common cancer types accompanied by rapidly increasing incidence rates, thus making the development of more efficient therapeutic approaches a necessity. Recent studies have revealed the potential role of decosahexaenoic acid ester of phloridzin (PZDHA) in suppressing proliferation of liver, breast, and blood cancer cell lines. In the present study, we investigated the cytotoxic potential of PZDHA in an in vitro model of skin cancer consisting of melanoma (A375), epidermoid carcinoma (A431), and non-tumorigenic (HaCaT) cell lines. Decosahexaenoic acid ester of phloridzin led to increased cytotoxicity in all cell lines as revealed by cell viability …