Medicine and Health Sciences Commons^™

Whole Genome Sequencing Of Moraxella Bovoculi Reveals High Genetic Diversity And Evidence For Interspecies Recombination At Multiple Loci, Aaron M. Dickey, Gennie Schuller, John D. Loy, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is frequently cultured from the ocular secretions and conjunctiva of cattle with Infectious Bovine Keratoconjunctivitis (IBK). Previous work has shown that single nucleotide polymorphism (SNP) diversity in this species is quite high with 81,284 SNPs identified in eight genomes representing two distinct genotypes isolated from IBK affected eyes (genotype 1) and the nasopharynx of cattle without clinical IBK signs (genotype 2), respectively. The goals of this study were to identify SNPs from a collection of geographically diverse and epidemiologically unlinked M. bovoculi strains from the eyes of IBK positive cattle (n = 183) and another from the eyes …

Differential Modulation Of Human Gabac‑Ρ1 Receptor By Sulfur‑Containing Compounds Structurally Related To Taurine, Lenin David Ochoa‑De La Paz, Martin González‑Andrade, Herminia Pasantes‑Morales, Rodrigo Franco, Rubén Zamora‑Alvarado, Edgar Zenteno, Hugo Quiroz‑Mercado, Roberto Gonzales‑Salinas, Rosario Gulias‑Cañizo

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: The amino acid taurine (2-Aminoethanesulfonic acid) modulates inhibitory neurotransmitter receptors. This study aimed to determine if the dual action of taurine on GABAC- ρ1R relates to its structure. To address this, we tested the ability of the structurally related compounds homotaurine, hypotaurine, and isethionic acid to modulate GABAC- ρ1R.

Results: In Xenopus laevis oocytes, hypotaurine and homotaurine partially activate heterologously expressed GABAC- ρ1R, showing an increment in its deactivation time with no changes in channel permeability, whereas isethionic acid showed no effect. Competitive assays suggest that hypotaurine and homotaurine compete for the GABA-binding site. In addition, their effects were …

Using Art And Story To Explore How Primary School Students In Rural Tanzania Understand Planetary Health: A Qualitative Analysis, Elizabeth Vanwormer, Jesca Mlawa, Elizabeth Komba, Christopher Gustafson, Hilda Mrema, Jenny M. Dauer

School of Veterinary and Biomedical Sciences: Faculty Publications

Background The global planetary health community increasingly recognises the need to prepare students to investigate and address connections between environmental change and human health. As we strive to support education on planetary health themes for students of all ages, understanding students’ concepts of linkages between the health of people and animals, and their shared environments might advance educational approaches. Children living in villages bordering Ruaha National Park in Iringa Region, Tanzania, have direct experience of these connections as they share a water-stressed but biodiverse environment with domestic animals and wildlife. Livelihoods in these villages depend predominantly on crop and livestock …

Tellurite Resistance In Shiga Toxin-Producing Escherichia Coli , Gentry L. Lewis, Quentin R. Jorgensen, John Dustin Loy, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Potassium tellurite ( K₂TeO₃) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K₂TeO₃ resistance in STEC is attributable to the ter gene cluster with terD an indicator of the cluster’s presence. Polymerase chain reactions for terD and K₂TeO₃ minimum inhibitory concentration (MIC) determinations in broth cultures were conducted on 70 STEC and 40 non-STEC control organisms. Sixty-six STEC strains (94.3%) were terD+ compared to 28 control organisms (70.0%; P < 0.001). The prevalence of terD in …

Epitope Mapping Of Serca2a Identifies An Antigenic Determinant That Induces Mainly Atrial Myocarditis In A/J Mice, Bharathi Krishnan, Chandirasegara Massilamany, Rakesh H. Basavalingappa, Arunakumar Gangaplara, Rajkumar Rajasekaran, Muhammad Z. Afzal, Vahid Khalilzad-Sharghi, You Zhou, Jean-Jack Riethoven, Shyam S. Nandi, Paras K. Mishra, Raymond A. Sobel, Jennifer L. Strande, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Sarcoplasmic/endoplasmic reticulum Ca²⁺ adenosine triphosphatase (SERCA)2a, a critical regulator of calcium homeostasis, is known to be decreased in heart failure. Patients with myocarditis or dilated cardiomyopathy develop autoantibodies to SERCA2a suggesting that they may have pathogenetic significance. In this report, we describe epitope mapping analysis of SERCA2a in A/J mice that leads us to make five observations: 1) SERCA2a contains multiple T cell epitopes that induce varying degrees of myocarditis. One epitope, SERCA2a 971–990, induces widespread atrial inflammation without affecting noncardiac tissues; the cardiac abnormalities could be noninvasively captured by echocardiography, electrocardiography, and magnetic resonance microscopy imaging. 2) SERCA2a …

The National Wildlife Strike Database: A Scientific Foundation To Enhance Aviation Safety, Richard A. Dolbeer, Michael J. Begier, John R. Weller

United States Department of Agriculture Wildlife Services: Staff Publications

The U.S. Federal Aviation Administration's (FAA) National Wildlife Strike Database (NWSD) documents reports of civil aircraft collisions with wildlife in USA. The NWSD has been managed by the Wildlife Services Program of the U.S. Department of Agriculture through an interagency agreement since its inception. Although the NWSD includes about 170,000 reports of civil aircraft collisions with wildlife (97% birds) from 1990-2015 (14,000 in 2015), the overriding focus has been the quality control of data entered for over 90 variables ranging from species and numbers of wildlife struck, location and time of day, phase and height of flight, aircraft type, components …

To Live And Fly In La: Using Bird Strike And Management Program Information To Improve Safety At Airports In The Los Angeles Basin, Todd J. Pitlik, Elizabeth Hermann, Eric Peralta, Brian E. Washburn

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife-aircraft collisions (wildlife strikes) pose a serious safety risk to aircraft. Wildlife strikes can be evaluated at different levels, include efforts to examine these problems at the national, regional, or state level, or for an individual airport. Similarly, wildlife strikes involving individual wildlife species or guilds can be examined at varying scales. Although wildlife strike analyses at the national, regional, or species/guild level are valuable, airport-specific analyses are essential for the effective implementation and evaluation of integrated wildlife damage management programs as these actions are conducted at the airport level. The species that present hazards to safe aircraft operations varies …

Follicular Responsiveness To Equine Chorionic Gonadotropin In Cat Species: Study In In Vitro Culture, Ajjima Chansaenroj

Chulalongkorn University Theses and Dissertations (Chula ETD)

Equine chorionic gonadotropin (eCG) has been commonly used to induce estrus in several felid species. However, the mechanisms by which this gonadotropin regulates cat folliculogenesis are still unclear. In this present study, we investigated 1) the in vitro responsiveness of cat ovarian follicles at different follicle developmental stages to various eCG concentration; 2) tiger antral follicle responsiveness to eCG; 3) the influence of eCG combination with insulin-like growth factor I (IGF-I) and/or stem cell factor (SCF) on cat ovarian follicles at different developmental stages. Study 1, the isolated follicles from the ovaries of 22 cats were classified into three developmental …

Application Of Needle-Free Injection Technology For Foot And Mouth Disease Vaccination Via Intradermal Route, Sirirat Wataradee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Foot and mouth disease (FMD) is an endemic disease in Thailand and caused severe economic loses. In order to prevent and control disease, the vaccination program is routinely administered for 2-3 times a year via subcutaneous route (SC). Even though, the vaccination is performed regularly, the outbreaks have often reported. The outbreaks occur due to the failure of number of immunity animal in population or herd immunity. The limitation of vaccine distribution to all animal population and difficulty of cattle restraint are forcible several farmers to failing to vaccinate their animals. Based on previous studies, the intradermal route (ID) can …

Growing South Dakota (Winter 2018), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Learn About Our Leaders
[Page] 4 Academic Programs at a Glace
[Page] 6 New Precision Ag Major Takes Off
[Page] 9 Raven Gives $5 Million Gift
[Page] 10 New Plant Science Research Facility
[Page] 13 Technical Training to Four-Year Degree
[Page] 15 Ag-Bio Career Fair Hits Record
[Page] 16 Breaking Ground : SD ADRDL
[Page] 19 Distinguished Professor Eric Nelson
[Page] 20 SDSU’s Veterinary Students
[Page] 24 Conservation Planning and Park Management
[Page] 25 Natural Resource Law Enforcement
[Page] 26 Medical Stethoscope Ceremony
[Page] 29 Master’s Degree in Human Biology
[Page] 30 Scott Pedersen Prepares Students
[Page] 32 …

Evaluation Of Pcr Primers Targeting The Vp2 Region Of The Foot-And-Mouth Disease Virus For Improved Serotype Detection, Waqas Ali, Mudasser Habib, Muhammad Salah Ud Din Shah

Turkish Journal of Veterinary & Animal Sciences

Frequently, a sequence from a single strain of a virus is used to design primers for PCR-based virus detection. However, high mutation rates in RNA viruses lead to failure of microorganism detection in clinical samples. Therefore, it is essential to find conserved regions within sequences to design primers for the detection of pathogen genomes in suspected clinical samples. The aim of the study was to find conserved regions of the genome for improved serotype detection. In the present study, primers were designed for serotype detection based on the VP2 coding region of foot-and-mouth disease virus (FMDV) after alignment of full …

Clinical Evaluation Of Topical Tacrolimus Ointment Usagein Different Stages Of Keratoconjunctivitis Sicca In Dogs, Christina John, Aswathy Gopinathan, Kiranjeet Singh, Pallvi Sharma, Chelladurai Sowbharenya, Sherin B. Sarangom

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effect of topical tacrolimus (0.1%) ointment in stimulating tear production in dogs with keratoconjunctivitis sicca (KCS). Thirty-six dogs (58 eyes) diagnosed with KCS were included in this study. KCS was grouped as early (E, n = 21 eyes), late (L, n = 21 eyes), and reflex tear due to corneal ulceration (R, n = 16 eyes) based on Schirmer tear test (STT) readings. The dogs in each group were randomly allotted treatment with either tacrolimus 0.1% ointment applied once daily (ET, n = 11 eyes; LT, n = 10 eyes; RT, n = …

Correlation Of Blood Triiodothyronine (T3) Level With Some Production Traits In Male Goat Kids, Khalid Abdoun, Emad Samara, Aly Okab, Mohamed Al-Badwi, Mohamed El-Zarei, Ali Al-Seaf, Ahmed Al-Haidary

Turkish Journal of Veterinary & Animal Sciences

Thyroid hormones are known for their stimulation of feed efficiency and growth performance in different farm animals. More interestingly, the level of thyroid hormones is influenced by selection for growth in some animal species. Therefore, it was the aim of the current study to investigate whether a correlation exists between the blood triiodothyronine (T 3 ) level and some production traits (daily feed intake, average daily gain, and feed conversion ratio). To test this hypothesis, six male goat kids (21.9 ± 2.4 kg; 5 months old) belonging to Aardi, Damascus, and their cross breed (two kids from each genotype) were …

Effects Of Different Extenders And Additives On Liquid Storage Of Awassi Ram Semen, Ömer Varişli, Abdullah Taşkin, Numan Akyol

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode lactate (TL-HEPES), and Dulbecco?s phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg yolk, …

Effects Of Organic And Inorganic Nitrogen Sources On In Vitro Degradability Of Citrus Byproduct And Milk Thistle, Asma Aggoun, Mohamed Laid Haddi, Si-Yong Yang, Jong-Su Eun, Andrea Squartini

Turkish Journal of Veterinary & Animal Sciences

In order to evaluate rumen microbial activity, the effects of two nitrogen sources (organic and inorganic) were analyzed in vitro by inoculating dairy cow rumen fluid and using citrus byproduct (CBP) and milk thistle (Silybum marianum, MT) as energy source substrates. The experimental design was 2 × 3 where we used three different nitrogen sources (N1: no nitrogen added, N: ammonium bicarbonate, N3: sodium glutamate) with a basic concentration of nitrogen (100 mg/1 L). Culture pH, dry matter degradability %, ammonia-N (NH$_{3}$-N), total volatile fatty acids (TVFAs), individual volatile fatty acid proportions, and acetate propionate ratio (A:P) were measured after …

Multi-Drug Resistant Escherichia Coli In Diarrhoeagenic Foals: Pulsotyping, Phylotyping, Serotyping, Antibiotic Resistance And Virulence Profiling, C.A. Kennedy, Ciara Walsh, M. Karczmarczyk, S. O'Brien, N. Akasheh, M. Quirke, S. Farrell-Ward, T. Buckley, U. Fogherty, K. Kavanagh, C.T. Parker, T. Sweeney, S. Fanning

Articles

Extraintestinal pathogenic E. coli (ExPEC) possess the ability to cause extraintestinal infections such as urinary tract infections, neonatal meningitis and sepsis. While information is readily available describing pathogenic E. coli populations in food-producing animals, studies in companion/sports animals such as horses are limited. In addition, many antimicrobial agents used in the treatment of equine infections are also utilised in human medicine, potentially contributing to the spread of antibiotic resistance determinants among pathogenic strains. The aim of this study was to phenotypically and genotypically characterise the multidrug resistance and virulence associated with 83 equine E. coli isolates recovered from foals with …

Physiological And Immunological Responses Of Porcine Primary Endometrial Cells To Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Infection, Muttarin Lothong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is highly limited to only cell subsets that express PRRSV receptors. Reproductive organ revealing the typical signs of PRRSV infection may be the critical site of problem syndromes. Persistent PRRSV producing re-infection via horizontal or vertical transmission could not be eradicated from herds. This research examined the possibility of porcine endometrium to be a PRRSV permissive cell and serves as the primary cause of the persistent PRRSV. Cellular and immunological in response to PRRSV relevant to viral replication, shedding and re-circulation was assessed. The different outcomes between the different genotypes (type I vs. …

Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main overall goal of this study is to investigate the application of modified chitosan as a potential vector for apoptotic gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells (i.e. testicular cells and mammary cancer cells). This study reported Gonadotropin Releasing Hormone-modified Chitosan (GnRH-CS) nanoparticle as a promising vector for targeted gene delivery, and a GnRH peptide was used for active targeting of a transgene in GnRHR expressing cells. The physiological characterizations of the prepared nanoparticle were investigated by proton nuclear magnetic resonance spectroscopy (1H NMR), Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Nanosizer. The prepared GnRH-CS was …

Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed …

The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya

Chulalongkorn University Theses and Dissertations (Chula ETD)

Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG …

Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka

Chulalongkorn University Theses and Dissertations (Chula ETD)

Synbiotics, a synergistic combination of probiotics and prebiotics, are now highlighted as one of the most practical nutritional supplements in red tilapia farms. The aim of the present study was to assess the effect of the dietary inclusion of Jerusalem artichoke, Lactobacillus rhamnosus, or their combination, on serum biochemical, growth performance, intestinal morphology, immune parameters, intestinal bacterial count and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.). One hundred and eighty male red tilapias (average body weight of 14.05±0.42 g) were distributed into six 1000-liter tanks containing water under continuous aeration and an average temperature of 25–28°C. Tilapias …

The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea is caused by porcine epidemic diarrhea virus (PEDV), which causes swine watery diarrhea and higher mortality rates in piglets less than one week of age. Outbreaks of PED affect to economic loss, especially in a swine industrial country. Many strategies were used for preventing the outbreak and infection of PEDV, especially maternal antibody stimulation in pigs and then transfer to piglet through colostrum or milk. Thus, the investigation of antibody level for the situation on the farm is required. The method which convenient and rapid than viral neutralization (VN) test is an enzyme-linked immunosorbent assay (ELISA). The …

Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brugia pahangi is a major causative agent of animal lymphatic filariasis, one of the neclected tropical disease. Recent studies have also indicated that B. pahangi can cause clinical infection in human, with clinical appearances which are consistent with human lymphatic filariasis. Nowadays, the recognition of Wolbachia as new alternative drug choice for treatment and control of lymphatic filariasis is the most important in anti-filarial chemotherapy. Since, Wolbachia play important roles, including the development, viability, fertility and fecundity of filarial parasites. Depletion of Wolbachia results in sterilization, stunting and death of filarial worms. In this study, we investigated the effects of …

Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Retinal (retinaldehyde) is one of natural vitamin A metabolites. It is widely used in clinical dermatology such as acne, acne scar, photoaging, wrinkles, psoriasis, skin neoplasia and seborrheic dermatitis. Retinal is a pivotal key to regulate keratinocyte proliferation and differentiation in the epidermis resulting in epidermal thickening and inhibit collagen destruction in the dermis. However, topical application of retinal is still irritative to the skin and chemically and photochemically unstable. Topically applied retinal can induce a retinoid dermatitis. This inflammation is induced by an overload of non-physiological amounts of exogenous retinoic acid in the skin. The adverse effects and physicochemical …

Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and …

Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain

Chulalongkorn University Theses and Dissertations (Chula ETD)

Live bird markets play a significant role in highly pathogenic avian influenza (HPAI) virus spread and transmission. In Myanmar, the first HPAI-H5N1 outbreak was reported in 2006. Since 2014, routine influenza A virus surveillance in LBMs have been conducted in Myanmar by the Livestock Breeding and Veterinary Department in cooperation with FAO. However, there is limited information on virus characterization and evolution of influenza viruses in Myanmar. The objectives of this thesis were to detect influenza virus subtypes circulating in different poultry species from Mingalar-Taung-Nyunt live bird market (MTN-LBM) and to determine genetic characteristics and genetic diversity of influenza A …

Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter has been known as an important cause of gastroenteritis in humans. The primary source is raw or undercooked poultry meat. Previous study showed that Campylobacter isolated from slaughterhouses were associated with Campylobacter isolated from broiler farms. Thus, detection of Campylobacter flock status and sorting flocks prior to slaughtering processes may help prevent Campylobacter contamination between Campylobacter-positive and negative flocks during slaughtering processes. Unfortunately, appropriate sample type and isolation method have not been clearly specified for Campylobacter detection at the farm level. Because Campylobacter are fastidious bacteria, improper detection method can attribute to false negative results. Therefore, the objectives of …

Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih

Chulalongkorn University Theses and Dissertations (Chula ETD)

Swine influenza causes high morbidity and low mortality in pigs. The disease spreads rapidly in pig farms causing the delay of pig weight to reach market. Moreover, pigs play important role as "mixing vessel" of influenza viruses. Pigs can support infection and replication of influenza viruses from avian, human and swine origins. These lead to major concerns of the important of swine influenza which are not only the economic impacts but also the human health. There is the need of accurate and rapid diagnosis for swine influenza virus (SIV) to minimize further spreading of the viruses in pig population and …

Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 220 pooled samples from 3 collection times were conducted. Eighty pooled samples from the first collection at 1 month after PED outbreak (1MAO) included 10 pooled oral fluid samples, 30 pooled fecal samples and 40 pooled surface swab samples. Eighty-five pooled samples from the second collection at 2MAO included 10 pooled oral fluid samples, 30 pooled fecal samples and 45 pooled surface swab samples. Fifty-five pooled samples from the third collection at 8MAO included 5 pooled oral fluid samples, 15 pooled fecal samples and 35 pooled surface swab samples. All samples collected from the PED-affected swine farm …

Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From​ Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 809 samples of pig rectal swab from slaughterhouses (n=441) and pork from retail market (n=368) were collected in the border provinces among Thailand, Cambodia, Lao PDR and Myanmar between October 2016 and March 2017. The objective of this study was to determine resistance to extended-spectrum ß-lactams (ESBLs) and colistin in Salmonella enterica and Escherichia coli. A total of Salmonella (n=463) and E. coli (n=767) were collected and determined for the ESBL-production and for the Minimum Inhibitory Concentrations (MICs) of colistin and; the presence of ESBL gene and mcr gene. The results showed that the prevalence of Salmonella …