Medicine and Health Sciences Commons^™

Phospholipase A2 Regulation Of Bovine Endometrial (Bend) Cell Prostaglandin Production, James D. Godkin, Mary P. Roberts, Mona Elgayyar, Wei Guan, Patricia K. Tithof

Animal Science Publications and Other Works

Background

Prostaglandins (PG), produced by the uterine endometrium, are key regulators of several reproductive events, including estrous cyclicity, implantation, pregnancy maintenance and parturition. Phospholipase A2 (PLA2) catalyzes the release of arachidonic acid from membrane phospholipids, the rate-limiting step in PG biosynthesis. The bovine endometrial (BEND) cell line has served as a model system for investigating regulation of signaling mechanisms involved in uterine PG production but information concerning the specific PLA2 enzymes involved and their role in regulation of this process is limited. The objectives of this investigation were to evaluate the expression and activities of calcium-dependent group IVA (PLA2G4A) and …

Missense Mutation In Exon 2 Of Slc36a1 Responsible For Champagne Dilution In Horses, Deborah Cook, Samantha Brooks, Rebecca Bellone, Ernest Bailey

Veterinary Science Faculty Publications

Champagne coat color in horses is controlled by a single, autosomal-dominant gene (CH). The phenotype produced by this gene is valued by many horse breeders, but can be difficult to distinguish from the effect produced by the Cream coat color dilution gene (CR). Three sires and their families segregating for CH were tested by genome scanning with microsatellite markers. The CH gene was mapped within a 6 cM region on horse chromosome 14 (LOD = 11.74 for theta = 0.00). Four candidate genes were identified within the region, namely SPARC [Secreted protein, acidic, cysteine-rich (osteonectin)], SLC36A1 (Solute Carrier 36 family …

Evaluación De La Inocuidad En La Cadena Cárnica Bovina En El Municipio De Cáqueza Cundinamarca, Yenifer Patricia Florez Parra, Andrés Hernando Sierra Pinillos

Medicina Veterinaria

No abstract provided.

Intraretinal Signaling By Ganglion Cell Photoreceptors To Dopaminergic Amacrine Neurons, Dao-Qi Zhang, Kwoon Y. Wong, Patricia J. Sollars, David M. Berson, Gary E. Pickard, Douglas G. Mcmahon

School of Veterinary and Biomedical Sciences: Faculty Publications

Retinal dopaminergic amacrine neurons (DA neurons) play a central role in reconfiguring retinal function according to prevailing illumination conditions, yet the mechanisms by which light regulates their activity are poorly understood. We investigated the means by which sustained light responses are evoked in DA neurons. Sustained light responses were driven by cationic currents and persisted in vitro and in vivo in the presence of L-AP4, a blocker of retinal ON-bipolar cells. Several characteristics of these L-AP4-resistant light responses suggested that they were driven by melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs), including long latencies, marked poststimulus persistence, and a peak …

Protection Of Retinal Cells From Ischemia By A Novel Gap Junction Inhibitor, Satyabrata Das, Dingo Lin, Snehalata Jena, Aibin Shi, Srinivas Battina, Duy H. Hua, Rachel A. Allbaugh, Dolores J. Takemoto

Rachel A. Allbaugh

Retinal cells which become ischemic will pass apoptotic signal to adjacent cells, resulting in the spread of damage. This occurs through open gap junctions. A class of novel drugs, based on primaquine (PQ), was tested for binding to connexin 43 using simulated docking studies. A novel drug has been synthesized and tested for inhibition of gap junction activity using R28 neuro-retinal cells in culture. Four drugs were initially compared to mefloquine, a known gap junction inhibitor. The drug with optimal inhibitory activity, PQ1, was tested for inhibition and was found to inhibit dye transfer by 70% at 10 μM. Retinal …

Questions And Answers: Keeping Herds Free From Bovine Tuberculosis And Protecting The Food Supply

Michigan Bovine Tuberculosis Bibliography and Database

Questions:
What can I do to keep my herd bovine tuberculosis (TB)-free?
How can I make sure that the animals I buy come from a TB-free herd?
How can I make sure that the animals I buy have not been exposed to a TB-infected herd?
Are new animals introduced to my stockyard a threat?
How do I protect calves from disease exposure?
If there is a TB outbreak in my area, are there any additional biosecurity measures, above and beyond best practices that I should take to reduce the chance of my cows getting the disease?
What biosecurity measures can …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

The Evaluation Of Selected 13 Polymorphic Microsatellite Markers For The Parentage Test Of Thai Holstein Cattle, Lerdchai Chintapitaksakul, Rapiphan Uavechanichkul

The Thai Journal of Veterinary Medicine

Nineteen families of 44 Thai Holstein cattle were evaluated for microsatellite characteristics and the parentage test. A total of 13 microsatellite markers were selected from the genome database and the genomic DNAs were isolated and used as templates for amplification. Multiplex PCR of these thirteen markers in a single reaction showed that only ten markers; b1, b2, b3, b4, b6, b7, b10, b11, b12 and b13 could amplify the fragments ranging from 5 to 12. Nine markers were highly informative with PIC of more than 0.5. An accuracy test revealed that seven markers (b2, b4, b6, b7, b10, b11 and …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Effects Of The Renin-Angiotensin Blockade On Renal Functions, Renal Norepinephrine Contents And Oxidative Stress In Cyclosporine Induced Nephrosis Rats, Suvaporn Panasjaroen, Sarinee Kalandakanond-Thongsong, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effects of renin-angiotensin blockade on renal function, renal norepinephrine (NE) contents and renal oxidative stress in Cyclosporine A (CsA) induced renal impairment in rats. Rats were assigned into three groups; group 1 (control group), receiving vehicle (propylene glycol) 1 ml/kg./day, subcutaneously (s/c). for 28 days; group 2 (CsA group), receiving CsA 15 mg/kg/day, s/c for 28 days; group 3 (CsA and losartan (LST) group), receiving CsA 15 mg/kg./day, s/c with the administration of LST 10 mg/k.g./day orally for 28 days. The results showed that CsA administration alone elevated mean arterial pressure (MAP), reduced …

Let’S Celebrate 30th Anniversary Of The Federation Of The Asian Veterinary Associations, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.

Treatment Of Infected Open Wounds On Two Dogs Using A Film Dressing Of Polysaccharide Extracted From The Hulls Of Durian (Durio Zibethinus Murr.): Case Report, Piyarat Chansiripornchai, Sunanta Pongsamart

The Thai Journal of Veterinary Medicine

There have been many reports on the efficacy of the polysaccharide peptidoglycan (PG) isolated from durian hulls in assisting wound healing in pig, dog, and cat skin. In this report, a two - year - old and a three -year - old female mongrel dogs with infected open wounds and skin necrosis were treated with PG film dressing. Complete wound healing in both dogs occurred within 14 weeks of treatment. PG film dressing may effectively promotes wound healing. The results show the benefit of using PG film dressings in pharmaceutical and veterinary applications.

Ultrastructures Of Red Blood Cells, White Blood Cells And Platelets In Four Species Of Macaques In Thailand, Kamol Sakulwira, Wimon Pothiwong, Pakorn Prachammuang, Siripen Vethchagarun

The Thai Journal of Veterinary Medicine

The structure of blood cells and platelets in 11 macaques (2 stump-tailed macaques, 5 long-tailed macaques, 1 rhesus macaque and 3 pig-tailed macaques) were studied using light and transmission electron microscopy (TEM). Erythrocytes were non-nucleated, biconcave disks without cytoplasmic organelles. Neutrophils had numerous small specific granules. Eosinophils contained specific granules with a dense matrix and a pale crystalloid body. Basophils revealed large round specific granules. Lymphocytes possessed scant cytoplasm with some mitochondria and azurophilic granules. Monocytes contained a large number of mitochondria, azurophilic granules and endoplasmic reticulum. Platelets were represented by anucleation and they had several different organelles in the …

Questions And Answers: Bovine Tuberculosis Signs And Symptoms

Michigan Bovine Tuberculosis Bibliography and Database

Questions:

What is bovine tuberculosis (TB)?
What animals and species can it affect?
What are the symptoms of TB?
What should I do if my animals show these symptoms?
How does TB get into cattle herds?
Are dairies more susceptible to TB?
How does APHIS test for TB?
How many animals in the United States are tested for TB each year?
Typically, when are animals tested for TB?
Does wildlife spread TB to commercial cattle? W
hat is the risk of TB transmission from local wildlife?
How is TB being managed in wildlife?
How can I find out if the …

Effect Of Supplementary Zinc From Organic And Inorganic Sources On Laying Performance And Zinc Deposition In Eggs, Hatairat Plaimast, Prapaisri Sirichakwal, Prapasri Puwastien, Suwanna Kijparkorn

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effect of supplementary organic and inorganic zinc on the laying performance and zinc deposition in eggs. One hundred and eighty laying hens (ISA Brown), aged 36 weeks, were randomly divided into 5 treatments with 4 replications of 9 hens each. A corn-soy based diet which was formulated to meet the recommended nutrient requirement especially the level of zinc (60 µµ µµ µg Zn/g diet) was used as the control diet. Both sources of zinc, inorganic (zinc sulfate) and organic (zinc amino acid chelate) were supplemented to provide zinc at levels of 300 and …

Effect Of Pentoxifylline On The Motility Characteristics And Viability Of Spermatozoa In Asian Elephants (Elephas Maximus) With Low Semen Quality, Nikorn Thongtip, Jumnian Saikhun, Sittidet Mahasawangkul, Kornchai Kornkaewrat, Piyawan Suthanmapinanh, Anuchai Pinyopummin

The Thai Journal of Veterinary Medicine

To investigate the effects of pentoxifylline (PTX) to enhance the motility and fertilization capacity of semen samples with the low-motile sperm in Asian elephants, fourteen semen collection attempts in 9 elephant bulls by manual stimulation were undertaken and eleven ejaculates fitted the criteria of investigation (0-30% motility). They were divided into two groups: poor-motile (0-9% motility) and low-motile (10-30% motility) sperm groups. Fresh semen samples were divided as a control group and 3 experimental groups that were supplemented with PTX at a final concentration of 0.5, 1.0 and 2.0 mg/ml, respectively. The semen samples were incubated at 37oC for 15 …

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

The Correlation Between Plasma L-Carnitine And Growth Performance In Suckling Pigs In Respect Of Mrna Expression Of A Carnitine Transporter, Octn2, In Lactating Porcine Mammary Tissues, Boonrit Thongsong, Sarinee Kalandakanond-Thongsong

The Thai Journal of Veterinary Medicine

Two objectives of this study were to investigate the weekly change in plasma L-carnitine concentration during the 28 day suckling period and to determine the relative mRNA expression level of a L-carnitine transporter, OCTN2, in porcine mammary tissue at early and peak lactation. The results showed that the L-carnitine concentration of piglets after giving birth was 3.94±0.13 mg/L. L-carnitine levels on day 7 were significantly decreased (p<0.05) compared to those on the 1st day. On day 14, L-carnitine level increased significantly (p<0.05) to the concentration of 3.99±0.55 mg/L and maintained this level until weaning day. As with the increase in plasma L-carnitine concentration 1 week following suckling age, the body weight also increased significantly (p<0.05). The linear correlation of suckling ages and L-carnitine levels has a significant and positive correlation (R2Adj=0.1328, p<0.05). The mRNA expression of OCTN2 found in these lactating sow mammary tissues showed the relative abundance of this carnitine transporter. It tended to be decreased in lactating mammary tissue of sows at their peak compared to those at early lactation (p=0.065). Therefore, the significant change or maintenance in plasma L-carnitine concentration and body weight gain during suckling period provided an insight into the effects of L-carnitine in suckling piglets. Additionally, the mRNA expression of OCTN2 in the lactating mammary cells will be further investigated in cellular and molecular regulatory mechanisms to transport L-carnitine from dams to their piglets via the mammary glands.

Regulation Of The Bioavailability Of Thioredoxin In The Lens By A Specific Thioredoxin-Binding Protein (Tbp-2), Namal P.M. Liyanage, M. Rohan Fernando, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Thioredoxin (TRx) is known to control redox homeostasis in cells. In recent years, a specific TRx binding protein called thioredoxin binding protein-2 (TBP-2) was found in other cell types and it appeared to negatively regulate TRx bioavailability and thereby control TRx biological function. In view of the sensitivity of lens transparency to redox status, proper regulation of TRx bioavailability is of the utmost importance. This study was conducted to examine the presence and function of TBP-2 in human lens epithelial cells (HLE B3). We cloned human lens TBP-2 from a human cDNA library (GenBank accession number AY 594328) and showed …

A Novel Application Of Quantile Regression For Identification Of Biomarkers Exemplified By Equine Cartilage Microarray Data, Liping Huang, Wenying Zhu, Christopher P. Saunders, James N. Macleod, Mai Zhou, Arnold J. Stromberg, Arne C. Bathke

Veterinary Science Faculty Publications

BACKGROUND: Identification of biomarkers among thousands of genes arrayed for disease classification has been the subject of considerable research in recent years. These studies have focused on disease classification, comparing experimental groups of effected to normal patients. Related experiments can be done to identify tissue-restricted biomarkers, genes with a high level of expression in one tissue compared to other tissue types in the body.

RESULTS: In this study, cartilage was compared with ten other body tissues using a two color array experimental design. Thirty-seven probe sets were identified as cartilage biomarkers. Of these, 13 (35%) have existing annotation associated with …

Sheep Updates 2008 - Contents, Department Of Food And Agriculture, Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2008 - Part 1, David Hughes, Tony Higgs, Di Evans, Scott Hansen, Michael Paton, Mary Nenke

Sheep Updates

This session covers five papers from different authors: KEYNOTE 1. Global trends in consumer and community expectations and their affect on the market, Dr David Hughes, Imperial College, London. (Powerpoint) PLENARY 2. Animal welfare - trends and opportunities, Tony Higgs, Di Evans, Department of Agriculture and Food WA 3. Australia's live sheep exports to Saudi Arabia, Scott Hansen, Meat & Livestock Australia 4. Livestock Welfare Challenges in Road Transport, Dr Mike Paton, Department of Agriculture and Food, Western Australia 5. My 2020 Summit Experience, Mary Nenke, Producer Kukerin WA

Bovine Viral Diarrhea Virus Multiorgan Infection In Two White-Tailed Deer In Southeastern South Dakota, Christopher C.L. Chase, Lyle J. Braun, Pamela Leslie-Steen, Tanya Graham, Dale Miskimins, Julia F. Ridpath

Veterinary and Biomedical Sciences Faculty Publications

The susceptibility of wild ruminants, especially cervids, to bovine viral diarrhea virus (BVDV) has remained an enigma. Two white-tailed deer (Odocoileus virginianus) were submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) in the fall of 2003 by the South Dakota Game Fish and Parks for chronic wasting disease (CWD) testing. Both animals were CWD negative. The animals were necropsied and histopathology, viral antigen detection, and virus isolation were performed. A noncytopathic (NCP) BVDV was isolated from the lungs and several other tissues of both animals. Formalin-fixed ear notches from both animals were positive for BVDV antigen by immunohistochemistry. …

Comparison Of The Contributions Of Heat-Labile Enterotoxin And Heat-Stable Enterotoxin B To The Virulence Of Enterotoxigenic Escherichia Coli In F4ac Receptor-Positive Young Pigs, Joseph Erume, Emil M. Berberov, Stephen D. Kachman, Michael A. Scott, You Zhou, David H. Francis, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

In swine, the most common and severe enterotoxigenic Escherichia coli (ETEC) infections are caused by strains that express K88 (F4)⁺ fimbriae, heat-labile enterotoxin (LT), heat-stable enterotoxin b (STb), and enteroaggregative E. coli heat-stable toxin 1. Previous studies based on a design that involved enterotoxin genes cloned into a nontoxigenic fimbriated strain have suggested that LT but not STb plays an important role in dehydrating diarrheal disease in piglets study, we compared these two toxins in terms of importance for piglets >1 week old with a study design that involved construction of isogenic single- and double-deletion mutants and inoculation of …

Sheep Updates 2008 - Part 2, Meredith L. Sheil, Di Evans, Brown Besier, Tim Scanlon, Andre Martinho De Almeida, Johan Greeff, Tanya Kilminster, John Milton, Chris Oldham, B. L. Mcintyre, Alison Wheatley, John Lucey, Fiona Jones, Nick Costa, Ed Riggall, J. R. L. Hall, Robin Jacob, Dave Pethick, Dave Hopkins, Graham Gardner

Sheep Updates

This session covers eleven papers from different authors: The Sheep Room 1. Analgesia for Surgical Husbandry Procedures in Sheep and Other Livestock, Dr Meredith L. Sheil, Animal Ethics Pty Ltd, Associate Sydney University Faculty of Veterinary Science The Wool Enterprise 2. Unmulsed sheep - implications for chemical use, Di Evans & Brown Besier, Department of Agriculture and Food WA 3. Are Damara and Dorper sheep better adapted than Merinos to nutritional stress? - Growth rates, Tim Scanlon1, Andre Martinho de Almeida2, Johan Greeff1, Tanya Kilminster1, John Milton3, Chris Oldham1, Department of Agriculture and Food WA1, Instituto de Investigacao Cientifica Tropical, …

Assessment Of Risk Associated With The Minnesota Proposed Plan For Split-State Status For Mycobacterium Bovis (Bovine Tuberculosis)

Michigan Bovine Tuberculosis Bibliography and Database

In July 2005, a Minnesota beef herd tested positive for Mycobacterium bovis (M. bovis) and was officially declared infected with bovine tuberculosis (TB). This was the first infected herd identified since Minnesota’s Accredited Free (AF) status was obtained in 1976. Subsequent testing identified infection in white-tailed deer and several cattle herds adjacent to this index herd. In February 2008, Minnesota declared its 11th infected cattle herd, resulting in the downgrade of the entire State’s TB status to Modified Accredited (MA).
In an effort to minimize the impact of MA status to the State and producers, Minnesota initiated the …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1- Partnerships Matter Diagnostic News [Page] 1- Bovine Lead Poisoning: May and June are Peak Months for Diagnosis [Page] 3- Holiday Hours [Page] 3- Water Suitability for Livestock [Page] 4- Why can’t you just run it anyway? Research News [Page] 4- Research Spotlight: Dr. Chris Chase Extension News [Page] 5- State Veterinary Loan Repayment Programs –a Regional Review [Page] 6- Pieces and Parts Student News [Page] 7- SDSU Pre-Veterinary Students Admitted to Veterinary Colleges for Fall 2008 [Page] 7- 2008 – 2009 Vet Science Scholarship Award Recipients [Page] 7- Calendar of Events

Known Facts About The Epidemiology Of Porcine Proliferative Enteropathy, Roberto M. C. Guedes

The Thai Journal of Veterinary Medicine

The amount of new information of the last seven years regarding Lawsonia intracellularis sensitivity to antimicrobial drugs, prevention/control based on treatment and vaccination, diagnosis and, finally, genome is amazing. Nevertheless, not much is known about the epidemiology of the disease, mainly regarding the source of infection, resistance of the bacteria in the environment and possible biological vectors that could spread the infection among farms. It was learned that as few as 1,000 to 10,000 bacteria are enough to induce infection and fecal shedding two to three weeks after inoculating susceptible pigs. It was also learned that L. intracellularis organisms can …

The Incidence On Severe Losses Of Piglets In Central Thailand Caused By The Ped Viral Disease, Supol Luengyosleuchakul

The Thai Journal of Veterinary Medicine

No abstract provided.