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Full-Text Articles in Medicine and Health Sciences
Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar
Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar
Turkish Journal of Veterinary & Animal Sciences
A total of 1500 serum samples were collected from cattle, buffaloes, and humans. The Rose Bengal plate test (RBPT) and enzyme-linked immunosorbent assay (ELISA) detected Brucella antibodies in 56 (10.18%) and 44 (8%) of the cattle, respectively. In samples collected from buffaloes 9.38% and 6.92% were positive, whereas 14% and 11% of the samples from humans were found to be positive by RBPT and ELISA, respectively. Among village workers a higher prevalence of brucellosis was recorded in females than in males. Abattoir-associated personnel also had a higher incidence of brucellosis. Results of the present study revealed that RBPT and ELISA …
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 100, and 1000 ng/ml EGF groups, as well as positive (EGF replaced with hCG) and negative (TCM 199 only, no supplement) control groups. Maturation and fertilisation rates were calculated at 39 °C in an atmosphere of …