Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Introducing Point Mutations Into The Atgs Of The Putative Open Reading Frames Of The Hsv-1 Gene Encoding The Latency Associated Transcript (Lat) Reduces Its Anti-Apoptosis Activity, Dale Carpenter, Gail A. Henderson, Chinhui Hsiang, Nelson Osorio, Lbachir Benmohamed, Clinton J. Jones, S L. Wechsler
Introducing Point Mutations Into The Atgs Of The Putative Open Reading Frames Of The Hsv-1 Gene Encoding The Latency Associated Transcript (Lat) Reduces Its Anti-Apoptosis Activity, Dale Carpenter, Gail A. Henderson, Chinhui Hsiang, Nelson Osorio, Lbachir Benmohamed, Clinton J. Jones, S L. Wechsler
School of Veterinary and Biomedical Sciences: Faculty Publications
The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene has anti-apoptosis activity that directly or indirectly enhances the virus’s reactivation phenotype in small animal models. The first 1.5 kb of the primary 8.3 kb LAT is sufficient and some or all of it is necessary for LAT’s anti-apoptosis in transient transfection assays and for LAT’s ability to enhance the reactivation phenotype. Based on LAT’s genomic sequence, the first 1.5 kb contains eight potential open reading frames (ORFs) defined as an ATG followed by an in frame termination codon. In this study, point mutations were introduced into the …
Bovine Herpesvirus Type 1 Induces Cell Death By A Cell Type Dependent Fashion, Vicki Geiser, Suzanne Rose, Clinton J. Jones
Bovine Herpesvirus Type 1 Induces Cell Death By A Cell Type Dependent Fashion, Vicki Geiser, Suzanne Rose, Clinton J. Jones
School of Veterinary and Biomedical Sciences: Faculty Publications
Bovine herpesvirus 1 (BHV-1), a member of the alpha-herpesvirinae subfamily, causes significant losses to the cattle industry. BHV-1 establishes latency in trigeminal ganglionic sensory neurons, but periodically reactivates from latency. Previous studies suggested that infection with BHV-1 induced novel morphological changes in rabbit skin cells (RS) versus bovine kidney cells (MDBK). Consequently, we hypothesized that viral infection led to a novel form of cell death in RS cells compared to MDBK cells. To test this hypothesis, we examined the levels of apoptosis in these cell types following infection with BHV-1. Infection of RS, but not MDBK, cells leads to high …