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Full-Text Articles in Medicine and Health Sciences

Distribution Of Edwardsiella Tarda Antigens And Igm Containing Cells In Tilapia Immune Organs During Septicemia: An Immunohistochemical Study, Nopadon Pirarat, Takayuki Katagiri, Masashi Maita, Makoto Endo, Achariya Sailasuta Jun 2008

Distribution Of Edwardsiella Tarda Antigens And Igm Containing Cells In Tilapia Immune Organs During Septicemia: An Immunohistochemical Study, Nopadon Pirarat, Takayuki Katagiri, Masashi Maita, Makoto Endo, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

An immunohistochemical study describes the localization of IgM containing cells involved in the primary immune organs of tilapia and the fate of E. tarda antigen distributed in the tissues during a course of intraperitoneal challenge. The E. tarda antigen was diffusely detected in various organs except the brain and spinal cord. The positive signals were identified within various cell types, including phagocytic cells, serosal mesothelia, myocardia, intestinal and gill epithelia and glomerular capillary endothelia. No extracellular deposition of bacterial antigen was observed. The bacterial antigen can persist up to 1 month in the granuloma and melano-macrophage center, confirming the intracellular …


A Collaborative Canadian–United Kingdom Evaluation Of An Immunohistochemistry Protocol To Diagnose Bovine Spongiform Encephalopathy, Lisa Manning, Katherine I. O'Rourke, Donald P. Knowles, Sarah A. Marsh, Yvonne I. Spencer, Estella Moffat, Gerald A. H. Wells, Stefanie Czub Jan 2008

A Collaborative Canadian–United Kingdom Evaluation Of An Immunohistochemistry Protocol To Diagnose Bovine Spongiform Encephalopathy, Lisa Manning, Katherine I. O'Rourke, Donald P. Knowles, Sarah A. Marsh, Yvonne I. Spencer, Estella Moffat, Gerald A. H. Wells, Stefanie Czub

Other Publications in Zoonotics and Wildlife Disease

Collaboration was established in 2001 to evaluate a commercially available immunohistochemistry assay kit for the detection of bovine spongiform encephalopathy (BSE) disease–associated prion protein in formic acid–treated formalin-fixed samples of bovine brain. The kit protocol was evaluated at the National Centre for Foreign Animal Diseases (Winnipeg, Canada) and the Veterinary Laboratories Agency (Weybridge, U.K.). The U.K. laboratory provided paraffin-embedded blocks of brainstem (medulla oblongata at the level of the obex) from 100 positive cases defined by clinical signs and histopathology, and 100 clinically suspect but BSE-negative samples defined by histopathology and immunohistochemistry with anti-PrP monoclonal antibody R145. The Canadian laboratory …


Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee Jan 2008

Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …


Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu Jan 2008

Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu

Turkish Journal of Veterinary & Animal Sciences

We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular …