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Articles 3301 - 3330 of 10753

Full-Text Articles in Botany

Bucklandiella Seppeltii, A New Species Of Grimmiaceae From Australasia, And Its Phylogenetic Position Based On Molecular Data, Halina Bednarek-Ochyra, Ryszard Ochyra, Jakub Sawicki, Monika Szczecinska Jan 2014

Bucklandiella Seppeltii, A New Species Of Grimmiaceae From Australasia, And Its Phylogenetic Position Based On Molecular Data, Halina Bednarek-Ochyra, Ryszard Ochyra, Jakub Sawicki, Monika Szczecinska

Turkish Journal of Botany

A new species, Bucklandiella seppeltii Bednarek-Ochyra, Ochyra, Sawicki & Szczecinska, is delimited by its distinct morphology and biogeography. The geographical range of the new species is restricted to the southern-temperate and southern-cool-temperate zones of Tasmania and New Zealand, with maximum occurrence on the South Island of the latter insular country. The species is described and illustrated and its distribution is mapped. The systematic position of B. seppeltii is revealed to be a sister to New Zealand B. allanfifei Bednarek-Ochyra & Ochyra by molecular evidence based on parsimony analysis of nuclear sequences. In some morphological and anatomical traits, B. seppeltii is …


First Karyotype Analysis, Physical Rdna Mapping, And Genome Size Assessment In 4 North African Astragalus Taxa (Fabaceae), Karim Baziz, Meriem Benamara-Bellagha, Fatima Pustahija, Spencer Craig Brown, Sonja Siljak-Yakovlev, Nadra Khalfallah Jan 2014

First Karyotype Analysis, Physical Rdna Mapping, And Genome Size Assessment In 4 North African Astragalus Taxa (Fabaceae), Karim Baziz, Meriem Benamara-Bellagha, Fatima Pustahija, Spencer Craig Brown, Sonja Siljak-Yakovlev, Nadra Khalfallah

Turkish Journal of Botany

Four taxa of Algerian Astragalus L. were studied for their chromosome number, karyotype features, and genome size. Fluorochrome banding was done for detection of GC-rich DNA regions, fluorescence in situ hybridization (FISH) for physical mapping of 35S and 5S rRNA genes, and flow cytometry for nuclear DNA content. All the taxa present the same chromosome number (2n = 2x = 16) and single 35S and 5S rDNA loci, but their distributions on chromosomes are different. The GC heterochromatin pattern was different among studied taxa and an unusually high number of chromomycin-positive bands were observed in A. pseudotrigonus Batt. & Trab. …


A Taxonomic Reassessment Of The Tanacetum Aureum (Asteraceae, Anthemideae) Species Group: Insights From Morphological And Molecular Data, Marzie Kazemi, Ali Sonboli, Hassan Zare Maivan, Shahrokh Kazempour Osaloo Jan 2014

A Taxonomic Reassessment Of The Tanacetum Aureum (Asteraceae, Anthemideae) Species Group: Insights From Morphological And Molecular Data, Marzie Kazemi, Ali Sonboli, Hassan Zare Maivan, Shahrokh Kazempour Osaloo

Turkish Journal of Botany

A taxonomic reassessment of the Tanacetum aureum (Lam.) Greuter, M.V.Agab. & Wagenitz (Asteraceae, Anthemideae) species group was performed. This group of species is distinguished by herbaceous perennial habit, (1-)2-10(-80) disciform capitula, with compact to lax corymbs and strongly to moderately convex receptacle. A multivariate statistical analysis of 58 morphological characters using Ward cluster, principal component analyses, and molecular phylogenetic analyses of nrDNA ITS and cpDNA rpL32-trnL^{(UAG)} + ndhF-rpL32 spacer sequence datasets were conducted. Both datasets corroborated that T. aureum, T. oligocephalum (DC.) Sch.Bip., and T. heimerlii (Nabelek) Parsa could be differentiated as separate species. An identification key of the studied …


Genetic Diversity Of Turkish Commercial Cotton Varieties Revealed By Molecular Markers And Fiber Quality Traits, Emi̇nur Elçi̇, Yaşar Akişcan, Batuhan Akgöl Jan 2014

Genetic Diversity Of Turkish Commercial Cotton Varieties Revealed By Molecular Markers And Fiber Quality Traits, Emi̇nur Elçi̇, Yaşar Akişcan, Batuhan Akgöl

Turkish Journal of Botany

To assess the genetic diversity and relationships among commercial Gossypium species released in Turkey between 1964 and 2014, 96 cotton varieties were analyzed using morphological and molecular markers. Morphological analysis was performed based on 4 fiber quality traits including fiber length, strength, fineness, and uniformity, and the mean values of each trait for each genotype were calculated using 2-year data. The results showed that most of the genotypes have long fiber length, very high fiber strength, coarse (45 genotypes) or average (50 genotypes) fiber fineness, and high uniformity. Twenty-six simple sequence repeat (SSR) markers and 14 markers linked to quantitative …


Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean, Simranpreet Kaur, Lowell D. Sandell, John L. Lindquist, Amit J. Jhala Jan 2014

Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean, Simranpreet Kaur, Lowell D. Sandell, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant giant ragweed is one of the most competitive weeds of agronomic crops in the United States. Early emergence and rapid growth rate makes giant ragweed a competitive weed early in the season and reduces crop yields. Therefore, early spring control of giant ragweed using a preplant herbicide is critical. Glufosinate is an alternative POST herbicide for weed control in glufosinate-resistant soybean. Field experiments were conducted at David City, NE, in 2012 and 2013 to evaluate the efficacy of preplant herbicides followed by glufosinate applied alone or in tank mixes for control of glyphosate-resistant giant ragweed in glufosinate-resistant soybean. Preplant …


The Evolutionary Divergence Of Pinus Nigra Subsp. Pallasiana And Its Varieties Based On Noncoding Trn Regions Of Chloroplast Genome, Aysun Demet Gülsoy, Ali̇ Murat Gülsoy, Burcu Çengel, Zeki̇ Kaya Jan 2014

The Evolutionary Divergence Of Pinus Nigra Subsp. Pallasiana And Its Varieties Based On Noncoding Trn Regions Of Chloroplast Genome, Aysun Demet Gülsoy, Ali̇ Murat Gülsoy, Burcu Çengel, Zeki̇ Kaya

Turkish Journal of Botany

The Anatolian black pine [Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe var. pallasiana] is one of the subspecies of European black pine, growing naturally as a widespread mid-elevation species in the southern, western, and northern Anatolian Mountains of Turkey. Three well-recognized varieties [var. pallasiana, var. fastigiata Businsky with pyramidal form, and var. seneriana (Saatçioğlu) Yalt. with globular-shaped crown with multiple stems] occur naturally, but the studies on them are very limited. These 3 varieties of Anatolian black pine were sampled in natural stands where they coexist to determine evolutionary divergence in the species as well as its evolutionary relationships with …


Allium Efeae (Amaryllidaceae), A New Species From Northwest Anatolia, Turkey, İlker Genç, Fatma Neri̇man Özhatay Jan 2014

Allium Efeae (Amaryllidaceae), A New Species From Northwest Anatolia, Turkey, İlker Genç, Fatma Neri̇man Özhatay

Turkish Journal of Botany

Allium efeae Özhatay & İ.Genç is described as a new species from northwest Anatolia. The distribution area of this species is restricted to Kıbrıscık (Bolu) and Beypazarı (Ankara). It resembles Allium lycaonicum Siehe, but differs in tepal shape and color, leaf length, and filament shape. A table comparing the diagnostic morphological characters of the new species and A. lycaonicum is provided. The diagnostic morphological characters, distribution, conservation status, and color photographs of the new species as well as its chromosome number (2n = 24) are presented.


A Revision Of Centaurea Sect. Centaurea (Asteraceae) From Iran, Massoud Ranjbar, Kazem Negaresh Jan 2014

A Revision Of Centaurea Sect. Centaurea (Asteraceae) From Iran, Massoud Ranjbar, Kazem Negaresh

Turkish Journal of Botany

A taxonomic review of Centaurea L. sect. Centaurea (Asteraceae) in Iran was carried out. Seven taxa are recognized and 1 of them is described as a new species: Centaurea joharchii Ranjbar & Negaresh. This new species has been described and illustrated from Khorasan Province, SE Iran. A new combination is proposed: C. kermanshahensis (Wagenitz) Ranjbar & Negaresh. In addition, C. aggregata Fisch. & C.A.Mey. ex DC. subsp. aggregata is typified and C. diffusa Lam. is reported here as a new record for Iran. Finally, a key to all species of the section Centaurea in Iran is provided.


Phylogeny, Geographic Distribution, And New Taxonomic Circumscription Of The Crocus Reticulatus Species Group (Iridaceae), Dörte Harpke, Lorenzo Peruzzi, Helmut Kerndorff, Theophanis Karamplianis, Theophanis Constantinidis, Vladimir Randelovic, Novica Randelovic, Marina Juskovic, Erich Pasche, Frank R. Blattner Jan 2014

Phylogeny, Geographic Distribution, And New Taxonomic Circumscription Of The Crocus Reticulatus Species Group (Iridaceae), Dörte Harpke, Lorenzo Peruzzi, Helmut Kerndorff, Theophanis Karamplianis, Theophanis Constantinidis, Vladimir Randelovic, Novica Randelovic, Marina Juskovic, Erich Pasche, Frank R. Blattner

Turkish Journal of Botany

Recent phylogenetic analyses proved several infrageneric units within the genus Crocus to be para- or polyphyletic. In an attempt to arrive at a system of Crocus that closely reflects species relationships, we provide here phylogenetic, morphometric, geographic, and nomenclatorial data for the species of a narrower-defined, monophyletic Crocus series Reticulati. We sequenced the ETS and ITS regions of the nuclear ribosomal DNA in 9 Reticulati and 19 outgroup species. Three chloroplast loci (trnL-F, rps16-trnQ, matK-trnK) were sequenced in the newly defined series Reticulati species and 1 outgroup. Data were analyzed with Bayesian and parsimony algorithms. The phylogenies resulted in 2 …


Heterologous Expression Of Esaba1 Enhances Salt Tolerance With Increased Accumulation Of Endogenous Aba In Transgenic Tobacco, Yujun Sun, Lin Zhu, Jiansheng Guo, Cheng Zhou Jan 2014

Heterologous Expression Of Esaba1 Enhances Salt Tolerance With Increased Accumulation Of Endogenous Aba In Transgenic Tobacco, Yujun Sun, Lin Zhu, Jiansheng Guo, Cheng Zhou

Turkish Journal of Botany

Salt stress can trigger several physiological responses in plants such as increased accumulation of ABA and antioxidant enzyme activities, which have been closely associated with the tolerance of plants to salt stress. In the present study a novel zeaxanthin epoxidase gene, designated EsABA1, was isolated from Eutrema salsugineum. EsABA1 contained a full-length open reading frame (ORF) of 2001 bp, encoding 666 amino acids. Transcription of EsABA1 rapidly accumulated after exposure to various abiotic stresses, suggesting that the EsABA1 gene was involved in multiple stress responses and tolerance. To further investigate the roles of EsABA1 under salt-stress conditions, transgenic tobacco lines …


A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan Jan 2014

A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Association analysis is an alternative to conventional family-based methods to detect the location of gene(s) or quantitative trait loci (QTL) and provides relatively high resolution in terms of defining the genome position of a gene or QTL. Seed protein and oil concentration are quantitative traits which are determined by the interaction among many genes with small to moderate genetic effects and their interaction with the environment. In this study, a genome-wide association study (GWAS) was performed to identify quantitative trait loci (QTL) controlling seed protein and oil concentration in 298 soybean germplasm accessions exhibiting a wide range of …


How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha Jan 2014

How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha

Department of Agronomy and Horticulture: Faculty Publications

Potential consequences of climate change on crop production can be studied using mechanistic crop simulation models. While a broad variety of maize simulation models exist, it is not known whether different models diverge on grain yield responses to changes in climatic factors, or whether they agree in their general trends related to phenology, growth, and yield. With the goal of analyzing the sensitivity of simulated yields to changes in temperature and atmospheric carbon dioxide concentrations [CO2], we present the largest maize crop model intercomparison to date, including 23 different models. These models were evaluated for four locations representing a wide …


Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang Jan 2014

Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.


Comparison Of Winter Strawberry Production In A Commercial Heated High Tunnel Versus A University Greenhouse, Ellen T. Paparozzi, Ryan Pekarek, George E. Meyer, Stacy A. Adams, M. Elizabeth Conley, David P. Lambe, Paul Read, Erin E. Blankenship Jan 2014

Comparison Of Winter Strawberry Production In A Commercial Heated High Tunnel Versus A University Greenhouse, Ellen T. Paparozzi, Ryan Pekarek, George E. Meyer, Stacy A. Adams, M. Elizabeth Conley, David P. Lambe, Paul Read, Erin E. Blankenship

Department of Agronomy and Horticulture: Faculty Publications

For the past 4 years, the University of Nebraska strawberry team has worked to develop low cost, sustainable methods for farmers and growers to produce strawberries in a double polyethylene greenhouse during the winter. This past year, this growing system was adapted to become a commercial grower’s heated high tunnel for the winter/spring of 2013-14. The idea was to scale up to a farm-size demonstration and compare it to the university greenhouse production system with a goal to expand marketing opportunities for strawberries into the winter season.


Switchgrass (Panicum Virgatum L) Flag Leaf Transcriptomes Reveal Molecular Signatures Of Leaf Development, Senescence, And Mineral Dynamics, Nathan A. Palmer, Teresa Donze-Reiner, David Horvath, Tiffany Heng-Moss, Brian M. Waters, Christian M. Tobias, Gautam Sarath Jan 2014

Switchgrass (Panicum Virgatum L) Flag Leaf Transcriptomes Reveal Molecular Signatures Of Leaf Development, Senescence, And Mineral Dynamics, Nathan A. Palmer, Teresa Donze-Reiner, David Horvath, Tiffany Heng-Moss, Brian M. Waters, Christian M. Tobias, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass flag leaves can be expected to be a source of carbon to the plant, and its senescence is likely to impact the remobilization of nutrients from the shoots to the rhizomes. However, many genes have not been assigned a function in specific stages of leaf development. Here, we characterized gene expression in flag leaves over their development. By merging changes in leaf chlorophyll and the expression of genes for chlorophyll biosynthesis and degradation, a four-phase molecular roadmap for switchgrass flag leaf ontogeny was developed. Genes associated with early leaf development were up-regulated in phase 1. Phase 2 leaves had …


Greenhouse Gas Emissions From Soil Treated With Dung Pats, Kenneth Evans, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Pamela Sutton, Kent M. Eskridge Jan 2014

Greenhouse Gas Emissions From Soil Treated With Dung Pats, Kenneth Evans, Ana Wingeyer, Martha Mamo, Walter H. Schacht, Pamela Sutton, Kent M. Eskridge

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.


Fate Of C And N From Dung Pats Into Soil, Kenneth Evans, Ana Wingeyer, Martha Mamo, Pamela Sutton, Jeff Bradshaw, Kent M. Eskridge, Matt Judkins, Jenna Beckmann, Erin Hatch Jan 2014

Fate Of C And N From Dung Pats Into Soil, Kenneth Evans, Ana Wingeyer, Martha Mamo, Pamela Sutton, Jeff Bradshaw, Kent M. Eskridge, Matt Judkins, Jenna Beckmann, Erin Hatch

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.


Quantitative Proteomic Analysis Of The Salmonella-Lettuce Interaction, Yuping Zhang, Renu Nandakumar, Shannon L. Bartelt-Hunt, Daniel D. Snow, Laurie Hodges, Xu Li Jan 2014

Quantitative Proteomic Analysis Of The Salmonella-Lettuce Interaction, Yuping Zhang, Renu Nandakumar, Shannon L. Bartelt-Hunt, Daniel D. Snow, Laurie Hodges, Xu Li

Department of Agronomy and Horticulture: Faculty Publications

Human pathogens can internalize food crops through root and surface uptake and persist inside crop plants. The goal of the study was to elucidate the global modulation of bacteria and plant protein expression after Salmonella internalizes lettuce. A quantitative proteomic approach was used to analyse the protein expression of Salmonella enterica serovar Infantis and lettuce cultivar Green Salad Bowl 24 h after infiltrating S. Infantis into lettuce leaves. Among the 50 differentially expressed proteins identified by comparing internalized S. Infantis against S. Infantis grown in Luria Broth, proteins involved in glycolysis were down-regulated, while one protein involved in ascorbate …


Quantitative Trait Loci (Qtl) That Underlie Scn Resistance In Soybean [Glycine Max (L.) Merr.] Pi438489b By ‘Hamilton’ Recombinant Inbred Line (Ril) Population, Kassem My Abdelmajid, Laura Ramos, D. L. Hyten, Jason Bond, Abdelhafid Bendahmane, Prakash R. Arelli, Victor N. Njiti, Silvia Cianzio, Stella K. Kantartzi, Khalid Meksem Jan 2014

Quantitative Trait Loci (Qtl) That Underlie Scn Resistance In Soybean [Glycine Max (L.) Merr.] Pi438489b By ‘Hamilton’ Recombinant Inbred Line (Ril) Population, Kassem My Abdelmajid, Laura Ramos, D. L. Hyten, Jason Bond, Abdelhafid Bendahmane, Prakash R. Arelli, Victor N. Njiti, Silvia Cianzio, Stella K. Kantartzi, Khalid Meksem

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode caused by Heterodera glycines Ichinohe is the most devastating pest in soybean [Glycine max (L.) Merr.]. Resistance to SCN is complex, polygenic, race and cultivar specific, and it is controlled by several quantitative trait loci (QTL). Our objective was to identify and map QTL for SCN resistance to races 3 (HG Type 0) and 5 (HG Type 2.5.7) using a high density SNP-based genetic linkage map based on the PI438489B by ‘Hamilton’ (PIxH, n=50) recombinant inbred line population. The PI438489B by Hamilton map contained 648 SNPs distributed on 31 LGs with coverage of 1,524.7 cM and …


Phytophthora Root Rot Resistance In Soybean E00003, Zhongnan Zhang, Jianjun Hao, Jiazheng Yuan, Qijian Song, D. L. Hyten, P. B. Cregan, Guorong Zhang, Cuihua Gu, Ming Li, Dechun Wang Jan 2014

Phytophthora Root Rot Resistance In Soybean E00003, Zhongnan Zhang, Jianjun Hao, Jiazheng Yuan, Qijian Song, D. L. Hyten, P. B. Cregan, Guorong Zhang, Cuihua Gu, Ming Li, Dechun Wang

Department of Agronomy and Horticulture: Faculty Publications

Phytophthora root rot (PRR) is a devastating disease in soybean [Glycine max (L.) Merr.] production. Michigan elite soybean E00003 is resistant to Phytophthora sojae and has been used as a resistance source in breeding. Genetic control of PRR resistance in this source is unknown. To facilitate marker-assisted selection (MAS), the PRR resistance loci in E00003 and their map locations need to be determined. In this study, a genetic mapping approach was used to identify major PRR -resistant loci in E00003. The mapping population consists of 240 F4–derived lines developed by crossing E00003 with the P. sojae susceptible …


Mapping The Low Palmitate Fap1 Mutation And Validation Of Its Effects In Soybean Oil And Agronomic Traits In Three Soybean Populations, Andrea J. Cardinal, Rebecca Whetten, Sanbao Wang, Jérôme Auclair, D. L. Hyten, P. B. Cregan, Eleni Bachlava, Jason Gillman, Martha Ramirez, Ralph Dewey, Greg Upchurch, Lilian Miranda, Joesph W. Burton Jan 2014

Mapping The Low Palmitate Fap1 Mutation And Validation Of Its Effects In Soybean Oil And Agronomic Traits In Three Soybean Populations, Andrea J. Cardinal, Rebecca Whetten, Sanbao Wang, Jérôme Auclair, D. L. Hyten, P. B. Cregan, Eleni Bachlava, Jason Gillman, Martha Ramirez, Ralph Dewey, Greg Upchurch, Lilian Miranda, Joesph W. Burton

Department of Agronomy and Horticulture: Faculty Publications

Soybean oil with reduced palmitic acid content is desirable to reduce the health risks associated with consumption of this fatty acid. The objectives of this study were: to identify the genomic location of the reduced palmitate fap1 mutation, determine its molecular basis, estimate the amount of phenotypic variation in fatty acid composition explained by this locus, determine if there are epistatic interactions between the fap1 and fapnc loci and, determine if the fap1 mutation has pleiotropic effects on seed yield, oil and protein content in three soybean populations. This study detected two major QTL for 16:0 content located in …


Identification Of New Qtls For Seed Mineral, Cysteine, And Methionine Concentrations In Soybean [Glycine Max (L.) Merr.], Raghuprakash Kastoori Ramamurthy, Joseph Jedlicka, George L. Graef, Brian M. Waters Jan 2014

Identification Of New Qtls For Seed Mineral, Cysteine, And Methionine Concentrations In Soybean [Glycine Max (L.) Merr.], Raghuprakash Kastoori Ramamurthy, Joseph Jedlicka, George L. Graef, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Increased concentrations of important nutrients in edible parts of plants could result in biofortified foods. Soybean [Glycine max (L.) Merr.] is a major legume crop and an important source of certain nutrients, including protein and minerals, in human and animal diets. Understanding the underlying genetic basis of seed composition is crucial to improving seed nutrient composition. In this study we used three soybean recombinant inbred line mapping populations derived from the crosses Williams 82 × DSR-173, Williams 82 × NKS19-90 and Williams 82 × Vinton 81, and constructed a joint linkage map from these populations. Forty quantitative trait loci (QTLs) …


The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf Jan 2014

The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf

Department of Agronomy and Horticulture: Faculty Publications

The difficulties in tackling climate change are inherently complex and primarily centered on political and social values. This is evident in the United States where political divisions and polarizations are fundamental barriers to advancing national policies, which in turn hinder international agreements, mitigation, and adaptation. Within the United States, the vast majority of agricultural and natural resource lands are projected to incur significant climate departures and are represented by the Republican Party. The resources and economic sectors that will be directly affected by climate change are represented by national leadership that is unlikely to accept policies to prevent or adapt …


Development Of Scar Markers And Up-Pcr Cross-Hybridization Method For Specific Detection Of Four Major Subgroups Of Rhizoctonia From Infected Turfgrasses, Bimal S. Amaradasa, Dilip Lakshman, Brandon J. Horvath, Keenan Amundsen Jan 2014

Development Of Scar Markers And Up-Pcr Cross-Hybridization Method For Specific Detection Of Four Major Subgroups Of Rhizoctonia From Infected Turfgrasses, Bimal S. Amaradasa, Dilip Lakshman, Brandon J. Horvath, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases on turfgrasses was developed based on the universally primed PCR (UPPCR) products cross-blot hybridization. Tester isolates belonging to the two varieties of W. circinata were amplified with a single UP primer L21, which generated multiple DNA fragments for each variety. Probes were prepared with UP-PCR products of each tester isolate by labeling with digoxigenin. Fieldcollected W. circinata isolates and representative isolates of different R. solani anastomosis groups (AG) and AG subgroups were amplified with L21, immobilized on nylon membrane and cross hybridized with …


Drought Effects On Composition And Yield For Corn Stover, Mixed Grasses, And Miscanthus As Bioenergy Feedstocks, Rachel Emerson, Amber Hoover, Allison Ray, Jeffrey Lacey, Marnie Cortez, Courtney Payne, Douglas L. Karlen, Stuart Birrell, David Laird, Robert Kallenbach, Josh Egenolf, Matthew Sousek, Thomas Voigt Jan 2014

Drought Effects On Composition And Yield For Corn Stover, Mixed Grasses, And Miscanthus As Bioenergy Feedstocks, Rachel Emerson, Amber Hoover, Allison Ray, Jeffrey Lacey, Marnie Cortez, Courtney Payne, Douglas L. Karlen, Stuart Birrell, David Laird, Robert Kallenbach, Josh Egenolf, Matthew Sousek, Thomas Voigt

Department of Agronomy and Horticulture: Faculty Publications

Drought conditions in 2012 were some of the most severe in recent history. The purpose of this study is to examine the impact of drought on quality, quantity, and theoretical ethanol yield (TEY) of three bioenergy feedstocks, corn stover, mixed grasses from Conservation Reserve Program lands, and Miscanthus × giganteus. To assess drought effects on these feedstocks, samples from 2010 (minimal to no drought) and 2012 (severe drought) were compared from multiple locations in the US. In all feedstocks, drought significantly increased extractives and reduced structural sugars and lignin; subsequently, TEYs were reduced 10–15%. Biomass yields were significantly reduced …


Nonsyntenic Genes Drive Highly Dynamic Complementation Of Gene Expression In Maize Hybrids, Anja Paschold, Nick B. Larson, Caroline Marcon, James C. Schnable, Cheng-Ting Yeh, Christa Lanz, Dan Nettleton, Hans-Peter Piepho, Patrick S. Schnable, Frank Hochholdinger Jan 2014

Nonsyntenic Genes Drive Highly Dynamic Complementation Of Gene Expression In Maize Hybrids, Anja Paschold, Nick B. Larson, Caroline Marcon, James C. Schnable, Cheng-Ting Yeh, Christa Lanz, Dan Nettleton, Hans-Peter Piepho, Patrick S. Schnable, Frank Hochholdinger

Department of Agronomy and Horticulture: Faculty Publications

Maize (Zea mays) displays an exceptional level of structural genomic diversity, which is likely unique among higher eukaryotes. In this study, we surveyed how the genetic divergence of two maize inbred lines affects the transcriptomic landscape in four different primary root tissues of their F1-hybrid progeny. An extreme instance of complementation was frequently observed: genes that were expressed in only one parent but in both reciprocal hybrids. This single-parent expression (SPE) pattern was detected for 2341 genes with up to 1287 SPE patterns per tissue. As a consequence, the number of active genes in hybrids exceeded that of …


Dt2 Is A Gain-Of-Function Mads-Domain Factor Gene That Specifies Semideterminacy In Soybean, Jieqing Ping, Yunfeng Liu, Lianjun Sun, Meixia Zhao, Yinghui Li, Zongxiang Tang, Hanh Nguyen, Zhixi Tian, Lijuan Qiu, Randall L. Nelson, Thomas E. Clemente, James Specht, Jianxin Ma Jan 2014

Dt2 Is A Gain-Of-Function Mads-Domain Factor Gene That Specifies Semideterminacy In Soybean, Jieqing Ping, Yunfeng Liu, Lianjun Sun, Meixia Zhao, Yinghui Li, Zongxiang Tang, Hanh Nguyen, Zhixi Tian, Lijuan Qiu, Randall L. Nelson, Thomas E. Clemente, James Specht, Jianxin Ma

Department of Agronomy and Horticulture: Faculty Publications

Similar to Arabidopsis thaliana, the wild soybeans (Glycine soja) and many cultivars exhibit indeterminate stem growth specified by the shoot identity gene Dt1, the functional counterpart of Arabidopsis TERMINAL FLOWER1 (TFL1). Mutations in TFL1 and Dt1 both result in the shoot apical meristem (SAM) switching from vegetative to reproductive state to initiate terminal flowering and thus produce determinate stems. A second soybean gene (Dt2) regulating stem growth was identified, which, in the presence of Dt1, produces semideterminate plants with terminal racemes similar to those observed in determinate plants. Here, we report positional …


Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed, Yung-Tsi Bolon, D. Hyten, James H. Orf, Carroll P. Vance, Gary J. Muehlbauer Jan 2014

Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed, Yung-Tsi Bolon, D. Hyten, James H. Orf, Carroll P. Vance, Gary J. Muehlbauer

Department of Agronomy and Horticulture: Faculty Publications

The complex network of regulatory factors and interactions involved in transcriptional regulation within the seed is not well understood. To evaluate gene expression regulation in the immature seed, we utilized a genetical genomics approach on a soybean [Glycine max (L.) Merr.] recombinant inbred line (RIL) population and produced a genome-wide expression quantitative trait loci (eQTL) dataset. The validity of the dataset was confirmed by mapping the eQTL hotspot for flavonoid biosynthesis-related genes to a region containing repeats of chalcone synthase (CHS) genes known to correspond to the soybean inhibitor locus that regulates seed color. We then identified eQTL for …


Nebraska’S Groundwater Legacy: Nitrate Contamination Beneath Irrigated Cropland, Mary E. Exner Spaulding, Aaron J. Hirsh, Roy F. Spalding Jan 2014

Nebraska’S Groundwater Legacy: Nitrate Contamination Beneath Irrigated Cropland, Mary E. Exner Spaulding, Aaron J. Hirsh, Roy F. Spalding

Department of Agronomy and Horticulture: Faculty Publications

A 31 year record of ~44,000 nitrate analyses in ~11,500 irrigation wells was utilized to depict the decadal expansion of groundwater nitrate contamination (N>10 mg/L) in the irrigated corn-growing areas of eastern and central Nebraska and analyze long-term nitrate concentration trends in 17 management areas (MAs) subject to N fertilizer and budgeting requirements. The 1.3 M contaminated hectares were characterized by irrigation method, soil drainage, and vadose zone thickness and lithology. The areal extent and growth of contaminated groundwater in two predominately sprinkler-irrigated areas was only ~20% smaller beneath well-drained silt loams with thick clayey-silt unsaturated layers and unsaturated …


Multilocation Corn Stover Harvest Effects On Crop Yields And Nutrient Removal, Douglas L. Karlen, Stuart J. Birrell, Jane M.F. Johnson, Shannon L. Osborne, Thomas E. Schumacher, Gary E. Varvel, Richard B. Ferguson, Jeff M. Novak, James R. Frederick, John M. Baker, John A. Lamb, Paul R. Alder, Greg W. Roth, Emerson D. Nafziger Jan 2014

Multilocation Corn Stover Harvest Effects On Crop Yields And Nutrient Removal, Douglas L. Karlen, Stuart J. Birrell, Jane M.F. Johnson, Shannon L. Osborne, Thomas E. Schumacher, Gary E. Varvel, Richard B. Ferguson, Jeff M. Novak, James R. Frederick, John M. Baker, John A. Lamb, Paul R. Alder, Greg W. Roth, Emerson D. Nafziger

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) stover was identified as an important feedstock for cellulosic bioenergy production because of the extensive area upon which the crop is already grown. This report summarizes 239 site-years of field research examining effects of zero, moderate, and high stover removal rates at 36 sites in seven different states. Grain and stover yields from all sites as well as N, P, and K removal from 28 sites are summarized for nine longitude and six latitude bands, two tillage practices (conventional vs no tillage), two stoverharvest methods (machine vs calculated), and two crop rotations {continuous corn (maize) …