Botany Commons^™

Scientific Note: Here The Whole Time: A Revised Understanding Of White Alumroot (Heuchera Alba, Saxifragaceae) In The Susquehanna River Valley., Anais Barnes, Madeline Wickers, Scott Schuette, Tanisha M. Williams, Ryan Folk, Christopher T. Martine

Faculty Journal Articles

Heuchera alba and H. pubescens (Saxifragaceae) are closely related species of alumroot endemic to the Appalachian region of eastern North America that can be difficult to distinguish morphologically. Heuchera pubescens has been understood to occupy a range from Kentucky to Pennsylvania, with the distribution of H. alba restricted to Virginia and West Virginia—plus a recently-recorded extension into Pennsylvania discovered with the help of social media. The uncovering of H. alba in Pennsylvania has challenged historical perceptions of Heuchera distributions in the state, particularly as this relates to the current concept of H. pubescens which may be in need of revision. …

A Simple And Affordable Protocol To Assess Censer Seed Dispersal: First Confirmation Of The Mechanism In The Genus Solanum, Abigail J. Motter, Christopher T. Martine, Melody P. Sain

Faculty Journal Articles

The censer seed dispersal mechanism, whereby mature fruits are retained on plants and seeds dispersed by mechanical shaking, is among the most specialized wind-aided seed dispersal strategies employed by the flowering plants. An efficient, affordable, and easily repeatable protocol for determining whether a species uses this unusual mechanism of seed dispersal is lacking. Our focal species, Solanum tudununggae, is one of just two Solanum species (in a fleshy-fruited genus of ca. 1200 species) hypothesized to use this dispersal mechanism as a means to move seeds farther from parent plants than would gravity alone.

A New Dioecious Bush Tomato, Solanum Nectarifolium (Solanaceae), From The Northern Tanami Desert, Northern Territory, Australia, With Reassessment Of S. Ossicruentum And A Change In The Circumscription Of S. Dioicum, Christopher T. Martine, Kym Brennan, Jason T. Cantley, Aiden Webb, Geoffrey Newton

Faculty Journal Articles

A new species of functionally dioecious bush tomato from the “Solanum dioicum + S. echinatum group” of Solanum subgenus Leptostemonum is described. Solanum nectarifolium Martine & Brennan, sp. nov., is a member of the taxonomically challenging Solanum dioicum W.Fitzg. species complex in Australia and differs from other species in the group by the presence of prominent and conspicuous extrafloral nectaries on the abaxial leaf surfaces, a tinge of purple on the new growth and young fruiting calyces, and curved prickles on young stems. Although fairly well represented in herbarium collections, this new taxon has historically been treated as a …

The Inherent Values Of Observation And Description: A Case Study In The Spiny Solanums Of Australia, Christopher T. Martine

Faculty Journal Articles

The basic exploratory effort of doing descriptive biology is an avenue by which we can deepen our knowledge about taxa and the natural phenomena that drive their diversification. As Conniff (2011) wrote in The species seekers, “we still live in the great age of discovery”, and much like that historically recognized period of the eighteenth and nineteenth centuries there remains today a distinct need for the sort of work that might have been attributable to the naturalists of that time. The careful observation of how organisms go about their lives, including how they interact with one another …

Scientific Note: Garden Loosestrife (Lysimachia Vulgaris, Primulaceae), An Emerging Invasive Plant Species Of Riparian Corridors In The Mid-Atlantic Region, Christopher T. Martine, Amy L. Jewitt, Claire M. Ciafre, Kaitlin G. Henry, Elizabeth H. Williams, Pj Newhart, Melody P. Sain

Faculty Journal Articles

Lysimachia vulgaris (garden loosestrife, Primulaceae) is an herbaceous perennial native to Europe that has been identified as an aggressive invasive species in parts of North America. The status of the species in the Mid-Atlantic region of the US, however, is poorly determined. Using recent field observations (from iNaturalist, iMapInvasives, and EDDMapS records) we here report localized incursions of L. vulgaris in the Susquehanna River Valley of central Pennsylvania, along with notes on its ecology, threats to native plant communities, and broader distribution in the state.

Genomic Structure Among Populations Of A Regionally Rare Perennial Plant Indicates The Need For Reevaluation Of Management Units, Cheyenne Moore, Angela J. Mcdonnell, Scott Schuette, Christopher T. Martine

Faculty Journal Articles

Conservation of rare species often relies on the delineation of management units and genomic tools can now be applied for this purpose. However, this is not as common on a local scale, where populations are often small and fragmented, despite the utility of informing conservation and management practice at the regional level. We use a genotyping by sequencing (GBS) approach to assess the perennial riparian plant species Baptisia australis (Fabaceae) in Pennsylvania, where the taxon is at the edge of its natural distribution and considered threatened. In this system, we investigate whether sampled subpopulations exhibit genetic structure. We find that …

The Aussie Oddballs: Identifying The Existence Of Censer And Trample Burr Dispersal In Australian Bush Tomatoes, Abigail Motter

Honors Theses

Australian bush tomatoes in northern Western Australia likely have seed dispersal mechanisms that depend on processes outside of frugivory, which is unusual for this genus. Solanum tudununggae seeds are wind dispersed by the censer mechanism, meaning that seeds fall from holes in the top of the fruit when the branches move. In another species, piles of abscised and burr-like fruits have been found strewn around the bases of S. ossicruentum, possibly due to the extinction of animals that may have been a means of dispersal via epizoochory. In this study, we attempt to provide support for the theorized dispersal …

Assessing Genetic Diversity And Population Structure In The Imperiled Aconitum Reclinatum (White Monkshood), An Endemic Wildflower Of Central Appalachia, Diamanda A. Zizis

Master’s Theses

Aconitum reclinatum, white monkshood (Ranunculaceae), is a Pennsylvania critically imperiled plant species found in five states of the Appalachian region of the United States: Pennsylvania, West Virginia, Virginia, North Carolina, and Tennessee. This plant is adapted to cool, moist environments but faces threats from increasing habitat loss, deer browse, logging, and changes in rainfall and temperature associated with climate change. Comprehensive knowledge of A. reclinatum genetic population structure remains limited. To address this, we collaborated with Natural Heritage programs within the range of A. reclinatum to sample populations across the species distribution and generate a genotyping-by-sequencing data set of …

Field-Based Courses Still Matter, But Not Like They Used To, Christopher T. Martine

Faculty Journal Articles

Increasingly, field-based courses are providing the first real opportunities for students to experience nature in meaningful ways. As a baseline, even before the content delivery and the graded assessments, this is already enough to change the way a person feels in the world and to spark an appreciation for the life around them.

(Part of a special feature on education in which recent winners of the Botanical Society of America's Charles E. Bessey Award share teaching philosophies and strategies.)

Seed Dispersal By Martu Peoples Promotes The Distribution Of Native Plants In Arid Australia, Rebecca Bliege Bird, Douglas Bird, Christopher T. Martine, Chloe Mcguire, Leanne Greenwood, Desmond Taylor, Tanisha M. Williams, Peter M. Veth

Faculty Journal Articles

Commensal relationships between wild plants and their dispersers play a key ecological and evolutionary role in community structure and function. While non-human dispersers are often considered critical to plant recruitment, human dispersers have received much less attention, especially when it comes to non-domesticated plants. Australia, as a continent historically characterized by economies reliant on non-domesticated plants, is thus a key system for exploring the ecological role of people as seed dispersers in the absence of agriculture. Here, we utilize a controlled observation research design, employing ecological surveys and ethnographic observations to examine how seed dispersal and landscape burning by Martu …

Scientific Note: An Incursion Of The Widely-Cultivated Katsura (Cercidiphyllum Japonicum, Cercidiphyllaceae) In Pennsylvania, Kayleigh R. Long, Melody P. Sain, Elizabeth H. Williams, Christopher T. Martine

Faculty Journal Articles

Cercidiphyllum japonicum (katsura) is a dioecious tree native to China and Japan. Although imperiled in parts of its natural distribution, the species has become quite common as a cultivated tree in temperate North America. The species is noted as occasionally escaping cultivation in the eastern United States, but few records of established incursions (and the conditions under which they might recruit) exist in the literature. We here report an incursion of C. japonicum in central Pennsylvania, along with notes on seedling recruitment on the Bucknell University campus.

Cross-Pollination: Building A Co-Taught Course To Examine Art And Sex Through The Lens Of Botany, Christopher T. Martine, Diamanda A. Zizis, Anna K. Kell

Faculty Journal Articles

Driven by overlapping interests in plants, art, and diversity in sex expression, Anna Kell (Department of Art and Art History) and Chris Martine (Department of Biology) developed a course that integrates the perspectives of a visual artist and a botanist. Art & Sex Through the Lens of Botany seeks to impart the importance of making connections across disciplines and the value of visual literacy across academic lines. The course introduces foundational concepts in each field and encourages students to integrate and explore these different systems of knowledge and their intersections. In addition to developing fluencies related to both general botany …

Genetic Diversity Of Paxistima Canbyi A. Gray: Conserving A Rare Plant Species Endemic To The Eastern United States, Isaac Buabeng

Master’s Theses

Premise: The glacial cycles of the Pleistocene period likely had a profound impact on the genetic diversity and phylogeographic patterns of numerous plant taxa in North America. However, the genetic diversity of rare, limestone-endemic woody species has not been highlighted. This study assesses genetic diversity in Paxistima canbyi A. Gray (Canby’s mountain lover or rat stripper), a rare limestone endemic small woody plant.

Methods: We investigated the genetic diversity of P. canbyi using genomic Single Nucleotide Polymorphism (SNP) data from 165 individuals from 14 populations sampled from across the entire range distribution. We first; de novo assembled the SNP …

Incorporating Sex-Diverse And Gender-Inclusive Perspectives In Higher Education Biology Courses, Breanne N. Harris, A Kelsey Lewis, Sam L. Sharpe, Teri J. Orr, Christopher T. Martine, Chloe C. Josefson

Faculty Journal Articles

Inclusive teaching is teaching in a way that reaches all students in the classroom; this is beneficial for everyone, particularly for those with minoritized identities. Instructors play a critical role in scaffolding how students are exposed to and learn science content in the classroom. In this manuscript, we discuss how biology instructors can make their classrooms more inclusive with regard to sex and gender diversity content. Many topics in biology are based on androcentric, heteronormative, and oppressive framing, even though those lenses are more reflective of our own history and culture than they are of the diversity we see in …

Morphological Trait Evolution In Solanum (Solanaceae): Evolutionary Lability Of Key Taxonomic Characters, Rebecca Hilgenhof, Edeline Gagnon, Sandra Knapp, Xavier Aubriot, Eric Tepe, Lynn Bohs, Leandro Giacomin, Yuri Gouvea, Andres Orejuela, Christopher T. Martine, Clara Ines Orozco, Iris E. Peralta, Tina Sarkinen

Faculty Journal Articles

Solanum is one of the world's largest and economically most important plant genera, including 1245 currently accepted species and several major and minor crops (e.g., tomato, potato, brinjal eggplant, scarlet eggplant, Gboma eggplant, lulo, and pepino). Here we provide an overview of the evolution of 25 key morphological traits for the major and minor clades of this giant genus based on stochastic mapping using a well-sampled recently published phylogeny of Solanum. The most evolutionarily labile traits (showing >100 transitions across the genus) relate to plant structure (growth form and sympodial unit structure), herbivore defence (glandular trichomes), pollination (corolla shape …

Fluidity And Inconstancy: Australian Bush Tomatoes As An Exemplar Of Non-Normative Sex Expression, Christopher T. Martine

Faculty Journal Articles

Solanum, a genus of ~1500 global species, is one of the more interesting plant groups in which to study reproductive biology and ecology. Overwhelmingly, species in this group express full cosexuality, where individual plants have flowers containing both fully-functioning “male” (staminate) and “female” (carpellate) organs. However, there have been multiple and widespread evolutionary transitions within the genus to non-normative variations on this ancestral condition. Australian bush tomatoes (ca. 40 species) are especially diverse in this regard, with uncommon variation and combinations of unisexuality and cosexuality -- including, most notably, two sexual systems known as dioecy (unisexual male or female …

A Foundational Population Genetics Investigation Of The Sexual Systems Of Solanum (Solanaceae) In The Australian Monsoon Tropics Suggests Dioecious Taxa May Benefit From Increased Genetic Admixture Via Obligate Outcrossing, Jason T. Cantley, Ingrid E. Jordon-Thaden, Morgan Roche, Daniel S. Hayes, Stephamie Kate, Christopher T. Martine

Faculty Journal Articles

Solanum section Leptostemonum is an ideal lineage to test the theoretical framework regarding proposed evolutionary benefits of outcrossing sexual systems in comparison to cosexuality. Theoretically, non-cosexual taxa should support more genetic diversity within populations, experience less inbreeding, and have less genetic structure due to a restricted ability to self-fertilize. However, many confounding factors present challenges for a confident inference that inherent differences in sexual systems influence observed genetic patterns among populations. This study provides a foundational baseline of the population genetics of several species of different sexual systems with the aim of generating hypotheses of any factor—including sexual system—that influences …

Nuclear Phylogeny And Insights Into Whole-Genome Duplications And Reproductive Development Of Solanaceae Plants, Jie Wang, Weibin Xu, Junwen Zhai, Yi Hu, Jing Guo, Caifei Zhang, Yiyong Zhao, Lin Zhang, Christopher T. Martine, Hong Ma, Chien-Hsun Huang

Faculty Journal Articles

Solanaceae, the nightshade family, have ∼2700 species, including the important crops potato and tomato, ornamentals, and medicinal plants. Several sequenced Solanaceae genomes show evidence for whole-genome duplication (WGD), providing an excellent opportunity to investigate WGD and its impacts. Here, we generated 93 transcriptomes/genomes and combined them with 87 public datasets, for a total of 180 Solanaceae species representing all four subfamilies and 14 of 15 tribes. Nearly 1700 nuclear genes from these transcriptomic/genomic datasets were used to reconstruct a highly resolved Solanaceae phylogenetic tree with six major clades. The Solanaceae tree supports four previously recognized subfamilies (Goetzeioideae, Cestroideae, Nicotianoideae, …

Phylogenomic Discordance Suggests Polytomies Along The Backbone Of The Large Genus Solanum, Edeline Gagnon, Rebeccca Hilgenhof, Andrés Orejuela, Angela J. Mcdonnell, Gaurav Sablok, Xavier Aubriot, Leandro Giacomin, Yuri Gouvêa, Thamyris Bragionis, João Renato Stehmann, Lynn Bohs, Steven Dodsworth, Christopher T. Martine, Péter Poczai, Sandra Knapp, Tiina Särkinen

Faculty Journal Articles

Premise of the study

Evolutionary studies require solid phylogenetic frameworks, but increased volumes of phylogenomic data have revealed incongruent topologies among gene trees in many organisms both between and within genomes. Some of these incongruences indicate polytomies that may remain impossible to resolve. Here we investigate the degree of gene-tree discordance in Solanum, one of the largest flowering plant genera that includes the cultivated potato, tomato, and eggplant, as well as 24 minor crop plants.

Methods

A densely sampled species-level phylogeny of Solanum is built using unpublished and publicly available Sanger sequences comprising 60% of all accepted species (742 spp.) …

Genetic Diversity & Connectivity Of Chasmanthium Latifolium (Poaceae) In Pennsylvania & The Effect On Conservation Status Of A Rare Species, Jonathan D. Hayes

Honors Theses

Chasmanthium latifolium (Michx.) Yates (Poaceae) is a loosely colonial, rhizomatous, perennial grass species that lives in riparian habitats, making it fittingly referred to as river oats. Native to the southern Midwest and the eastern half of the United States, C. latifolium reaches the northeastern edge of its range in Pennsylvania. Within Pennsylvania, eleven extant C. latifolium populations are found along four waterways: the Monongahela River, the Susquehanna River, and two tributaries to the Susquehanna River. This limited state distribution exhibits an east-west disjunct distribution, where western populations are largely separated from eastern populations with one centrally located population. Between the …

Lepidopteran Granivory Reduces Seed Counts In A Rare Species Of Riparian Scour Prairies, Cheyenne Moore, Angela J. Mcdonnell, Scott Schuette, Christopher T. Martine

Faculty Journal Articles

In Pennsylvania Baptisia australis var. australis is found along only four waterways: the Allegheny River, Youghiogheny River, Clarion River, and Red Bank Creek. Because of its limited distribution and small number of extant populations, the species is considered state-threatened in Pennsylvania. In addition, the riparian prairie habitat that Pennsylvania Baptisia australis var. australis is restricted to is also in decline and considered vulnerable. Because of these conservation concerns, insights into the natural history of the taxon in the state is valuable and will inform conservation efforts. Field surveys and fruit collections along the Allegheny River and herbarium collections were used …

Investigation Into The Genetic Provenance Of Three Rare Plants With East-West Disjunction Patterns In Pennsylvania., Scott Schuette, Christopher T. Martine

Other Faculty Research and Publications

Rare plant conservation relies on an understanding of the natural history, biology and ecology, and real and potential threats to their populations to inform state regulations that serve to protect the species from extirpation. This work often involves extensive field surveys over several years to determine population sizes and whether those populations are seeing reductions in number of individuals necessary to maintain the genetic diversity within and between those populations. Species and populations with high genetic diversity are better equipped to withstand sudden changes to their habitats that derive from land use changes and changing climate. There are a variety …

A Basic Ddradseq Two‐Enzyme Protocol Performs Well With Herbarium And Silica‐Dried Tissues Across Four Genera, Ingrid E. Jordon-Thaden, James B. Beck, Catherine A. Rushworth, Michael D. Windham, Nicolas Diaz, Jason T. Cantley, Christopher T. Martine, Carl J. Rothfels

Faculty Journal Articles

Premise

The ability to sequence genome‐scale data from herbarium specimens would allow for the economical development of data sets with broad taxonomic and geographic sampling that would otherwise not be possible. Here, we evaluate the utility of a basic double‐digest restriction site–associated DNA sequencing (ddRADseq) protocol using DNAs from four genera extracted from both silica‐dried and herbarium tissue.

Methods

DNAs from Draba, Boechera, Solidago, and Ilex were processed with a ddRADseq protocol. The effects of DNA degradation, taxon, and specimen age were assessed.

Results

Although taxon, preservation method, and specimen age affected data recovery, large phylogenetically informative …

Temperate Eurasian Origins Of Hawaiian Chenopodium (Amaranthaceae) Plus Description Of A New Species Endemic To Moloka‘I, Jason T. Cantley, Angela J. Mcdonnell, J Branson, S R. Long, W Garnett, Christopher T. Martine

Faculty Journal Articles

Chenopodium taxa of Hawai‘i are tetraploids distinguished from other members of the circumglobally distributed genus by minute morphological characters. Because of these reasons, the geographic origin of Hawaiian Chenopodium has remained unclear. Across the Hawaiian Archipelago, Chenopodium taxa are morphologically variable and grow in highly disparate xeric habitats, especially in terms of precipitation, temperature, wind, salt spray, and solar irradiation. Habitats include dry subalpine shrublands, sandy beach strand of atolls in the Northwest Hawaiian Islands, dry forests, and precipitously tall sea cliffs of northwestern Moloka‘i. From the Moloka‘i sea cliffs, which are battered by high energy winds, salt spray, and …

Integrated Pest Management In The Academic Small Greenhouse Setting: A Case Study Using Solanum Spp. (Solanaceae)., Daniel S. Hayes, Ingrid E. Jordon-Thaden, Jason T. Cantley, Angela J. Mcdonnell, Christopher T. Martine

Faculty Journal Articles

Premise

Botanical faculty and staff at academic institutions are often tasked with establishing and/or caring for plant collections held in small greenhouse facilities. Once plants are in place, an especially acute challenge is managing plant pest/pathogen populations. Integrated pest management (IPM) approaches are an excellent option, but few examples exist in the literature of successful programs that have been developed in academic small greenhouse settings.

Methods and Results

Over several years, we developed an IPM program for two small research greenhouses on the campus of a primarily undergraduate institution where hundreds of plants have been grown for studies in the …

Solanum Plastisexum, An Enigmatic New Bush Tomato From The Australian Monsoon Tropics Exhibiting Breeding System Fluidity., Angela J. Mcdonnell, Heather B. Wetreich, Jason T. Cantley, Peter Jobson, Christopher T. Martine

Faculty Journal Articles

A bush tomato that has evaded classification by solanologists for decades has been identified and is described as a new species belonging to the Australian “Solanum dioicum group” of the Ord Victoria Plain biogeographic region in the monsoon tropics of the Northern Territory. Although now recognised to be andromonoecious, S. plastisexum Martine & McDonnell, sp. nov. exhibits multiple reproductive phenotypes, with solitary perfect flowers, a few staminate flowers or with cymes composed of a basal hermaphrodite and an extended rachis of several to many staminate flowers. When in fruit, the distal rachis may abcise and drop. A member of …

Phylogeny Of The Australian Solanum Dioicum Group Using Seven Nuclear Genes: Testing Symon’S Fruit And Seed Dispersal Hypotheses., Christopher T. Martine, Ingrid E. Jordon-Thaden, Angela J. Mcdonnell, Jason T. Cantley, Daniel S. Hayes, Morgan D. Roche, Emma S. Frawley, Ian S. Gilman, David C. Tank

Faculty Journal Articles

The dioecious and andromonoecious Solanum taxa (the “S. dioicum group”) of the Australian Monsoon Tropics have been the subject of phylogenetic and taxonomic study for decades, yet much of their basic biology is still unknown. This is especially true for plant-animal interactions, including the influence of fruit form and calyx morphology on seed dispersal. We combine field/greenhouse observations and specimen-based study with phylogenetic analysis of seven nuclear regions obtained via a microfluidic PCR-based enrichment strategy and high-throughput sequencing, and present the first species-tree hypothesis for the S. dioicum group. Our results suggest that epizoochorous trample burr seed …

Heuchera Alba Twitter Identification, Christopher T. Martine

Other Faculty Research and Publications

The casual posting of a misidentified photograph led to a tweet exchange among Chris Martine, Bucknell University; Ryan Folk, Florida Museum of Natural History; and Scott Schuette, Pennsylvania Natural Heritage Program in June 2017. The scientists used their private accounts to conduct a discussion about the specific species identified. This exchange opened the possibility of a previously unknown range of a rare species, which Martine explored further and documented in the scientific publication PhytoKeys.

Tweet id: 873120823464218624

user ID: @MartineBotany

Related records included in this repository are "The hidden Heuchera: How science Twitter uncovered a globally imperiled species in Pennsylvania, …

Museum Collections: Natural History Training Bridges Time, Space, And Digital Platforms., Anna Monfils, Karen Powers, Christopher J. Marshall, James F. Smith, Chris Martine, L. Alan Prather

Faculty Journal Articles

Natural history collections offer a number of unique physical and virtual opportunities to create formal and informal progressive learning environments. Collections provide direct interaction with biodiversity as it changes through time and space. Collections-based experiences lead to an increased understanding and substantive interaction with the living world. Recent studies demonstrate how nature and outdoor experiences can improve learning. We discuss how collections, and the data associated with collections, are a critical component linking nature and scientific inquiry. Partnerships that develop around collections and collections-based science can foster innovative educational and research experiences that are enhanced by access to museum specimens. …

Age-Old Palms On Florida’S Ancient Ridges, Warren G. Abrahamson Ii

Faculty Journal Articles

Genetic fingerprinting, ramet growth rate determinations, and mathematical modeling (Minimum branching trees) were used to estimate clone ages of saw palmettos at Archbold Biological Station in Florida. Growth rates indicate that 500 year old saw palmetto ramets are common. Genetically identified clones (genets) are estimated to be thousands of years old, likely exceeding 5,000 years of age.