Pharmacology Commons^™

The Role Of Androstane Neurosteroids In Alcohol-Mediated Social Behavior, Jason J. Paris

Legacy Theses & Dissertations (2009 - 2024)

Androgenic steroids, such as testosterone, may influence the propensity for aggression in both people and animals. Some of testosterone's effects on aggression may be due, in part, to its metabolic products that are 5á-reduced and 3á-hydroxylated to form, 3á-androstanediol (3á-diol), which can also enhance aggression in mice. Notably, alcohol (EtOH) consumption facilitates aggression in people and animals, particularly among those predisposed to act aggressively. In rats, EtOH can increase 3á-diol in prefrontal cortex, which may facilitate aggression. The present work aimed to elucidate the role of 3á-diol for EtOH-enhanced aggression. We hypothesized that EtOH would enhance inter-male aggression, social dominance, …

In Vitro And In Vivo Pharmacodynamic Characterization Of The Novel Plasma Kallikrein Inhibitor Pf-04886847, Dhaval Sanjeev Kolte

Electronic Theses and Dissertations

Plasma kallikrein plays an important role in the pathogenesis of inflammation and thrombosis. Kallikrein cleaves high molecular weight kininogen (HK) to liberate the potent pro-inflammatory peptide bradykinin (BK). BK upon activation of its constitutive B2 receptors on endothelial cells leads to an increase in intracellular Ca2+ level and subsequent production of nitric oxide (NO) and prostacyclin (PGI2), ultimately leading to vasodilation, hypotension, increased vascular permeability and vascular leakage. Further, kallikrein activates the complement system and stimulates neutrophil chemotaxis, aggregation and elastase release. Furthermore, kallikrein mediates the conversion of factor XII (FXII) to activated factor XII (FXIIa) thereby potentiating the intrinsic …

Electro-Gene Transfer To Skin Using A Noninvasive Multielectrode Array, Siqi Guo, Amy Donate, Gaurav Basu, Cathryn Lundberg, Loree Heller, Richard Heller

Bioelectrics Publications

Because of its large surface area and easy access for both delivery and monitoring, the skin is an attractive target for gene therapy for cutaneous diseases, vaccinations and several metabolic disorders. The critical factors for DNA delivery to the skin by electroporation (EP) are effective expression levels and minimal or no tissue damage. Here, we evaluated the non-invasive multielectrode array (MEA) for gene electrotransfer. For these studies we utilized a guinea pig model, which has been shown to have a similar thickness and structure to human skin. Our results demonstrate significantly increased gene expression 2 to 3 logs above injection …

The Effects Of Protein Kinase C Inhibitors On Blood Nitric Oxide And Hydrogen Peroxide Release In Ischemia And Reperfusion Injury, Kyle D. Bartol

PCOM Biomedical Studies Student Scholarship

Vascular endothelial dysfunction is a key component initiating oxidative stress in ischemia/reperfusion (I/R). Endothelial dysfunction is characterized by an increase in hydrogen peroxide (H2O2) and a decrease in the bioavailability of nitric oxide (NO). Previous studies using protein kinase C (PKC) inhibitor Gö 6983 or PKC Beta (β) II inhibitor improved cardiac function in myocardial I/R, decreased leukocyte-endothelial interactions and leukocyte superoxide (SO) release and increased endothelial-derived NO release in vitro. This study examined the effects of Gö 6983 or PKC β II inhibitor on realtime H2O2 and NO release in femoral vein I/R in vivo. NO or H2O2 microsensors …

Feedback From Malaysian Pharmacists Who Underwent A 3-Year Period Of Compulsory Service With The Government, Ong Aik Liang, Thomas Paraidathathu

The Thai Journal of Pharmaceutical Sciences

Since 2004, it is mandatory for all new pharmacy graduates in Malaysia to serve the government for a period of 4 years, before they are free to work as registered pharmacists in a sector of their choice, either public or private. With the enactment of th...

Sensitive, Selective And Extraction-Free Spectrophotometric Determination Of Lamotrigine In Pharmaceuticals Using Two Sulphonthalein Dyes, K. B. Vinay, H. O., N. Rajendraprasad, K. Basavaiah

The Thai Journal of Pharmaceutical Sciences

Two direct, simple, sensitive and rapid extraction-free spectrophotometric methods have been developed for the determination of lamotrigine (LMT) in pure form and in its dosage forms. The methods are based on formation of ion-pair complex between the dru...

Pharmacotherapy Safety And Efficacy In Adolescent Smoking Cessation, Jennifer Lincoln

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: To determine whether or not pharmacotherapy is a safe and effective treatment option for smoking cessation in adolescents

Small Gtp-Binding Proteins In Insulin Secretion, Bhavaani Jayaram

Wayne State University Dissertations

Type 2 diabetes mellitus is marked by a substantial beta-cell failure which is characterized by defective insulin secretion and resistance to insulin. Understanding the molecular events leading to Glucose-stimulated insulin secretion [GSIS] might serve as therapeutic potential towards diabetes. GSIS involves interplay between small G-proteins and their regulatory factors. Herein, I tested the hypothesis that Arf nucleotide binding site opener [ARNO], a guanine nucleotide exchange factor [GEF] for the small G-protein Arf6, mediates the activation of Arf6, and that ARNO/Arf6 signaling axis, in turn, controls the activation of downstream effectors. Salient features of my study are: [i] ARNO/Arf6 is expressed …

Mechanisms Of Regulation Of Islet Function By Nadph Oxidase, Ismail Syed

Wayne State University Dissertations

Glucose stimulated insulin secretion (GSIS) involves a series of metabolic and cationic events, leading to translocation of insulin-laden secretory granules from a distal site toward the plasma membrane for fusion and release of insulin into circulation. Vesicular transport and fusion events are tightly regulated by signals which coordinate between vesicle- and membrane-associated docking proteins. It is now being accepted that reactive oxygen species [ROS] plays a second messenger role in islet â-cell function. Further, evidence from multiple laboratories suggests a tonic increase in ROS generation is necessary for GSIS and fatty acid-induced insulin secretion. On the other hand, excessive ROS …

Synthesis And Cytotoxicity Study Of Magnetoliposomes Loaded Doxorubicin, Surachai Ngamratanapaiboon, Issara Sramala, Wichet Leelamanit

The Thai Journal of Pharmaceutical Sciences

The limitation of clinical use of doxorubicin is its side effects especially a cumulative dose-dependent cardiotoxicity. To overcome this, doxorubicin can be targeted to accumulate on the site of action and limit dispersion in healthy tissue. Magnetic li...

Effects Of Marmin Isolated From Aegle Marmelos Correa On L-Histidine Decarboxylase Enzyme In Rbl-2h3 Cells, Agung Endro Nugroho, Novrizal Abdi, Sugeng Riyanto, Kazutaka Maeyama, Zullies Ikawati

The Thai Journal of Pharmaceutical Sciences

Marmin or (7-(6’,7’-dihydroxygeranyl-oxy) coumarin is a compound isolated from Aegle marmelos Correa, in the study, we investigated the effects of marmin on the L-histidine decarboxylase (HDC), a rate-limiting histamine synthesizing enzyme, in rat ma...

Rapid Titrimetric And Spectrophotometric Assay Of Tramadol In Bulk Drug And In Formulation Using N-Bromosuccinimide And Methyl Orange As Reagents, K. B. Vinay, H. D., N. Rajendraprasad, M. S. Raghu, P. J. Ramesh, M. X. Cijo, K. Basavaiah

The Thai Journal of Pharmaceutical Sciences

Tramadol is a centrally acting analgesic used for prevention and treatment of moderate to severe pain. Two simple, rapid and sensitive methods by titrimetric and spectrophotometric techniques are described for the determination of tramadol hydrochloride ...

Allylmercaptocaptopril Attenuates Sodium Selenite Induced Experimental Cataractogenesis: An In Vitro And In Vivo Study, K. S. Lakshmi, Shrinivas Sharma

The Thai Journal of Pharmaceutical Sciences

The present study sought to assess the efficacy of the allylmercaptocaptopril เท preventing seleniteinduced cataractogenesis in an experimental setting. The first, in vitro phase of the study was performed on lenses from Wistar rats incubated for 24 ...

Design And Characterization Of Bioadhesive Ophthalmic Films Of Flurbiprofen, Rashi Saroot, Ritu Mehra Gilhotra

The Thai Journal of Pharmaceutical Sciences

An attempt has been made to formulate sustained release ophthalmic films of flurbiprofen along with bioadhesive polymers. Ocular films were prepared by using polymers; sodium alginate, chitosan and HPMC; alone and in different combinations. Glycerol was ...

Influence Of Surfactants On Crystal Form Of Mefenamic Acid, Kaival P., Kulkarni P.K., Mudit Dixit, Ashwini G. Kni

The Thai Journal of Pharmaceutical Sciences

Mefenamic acid is an anti inflammatory and poorly water-soluble drug. Since the drug powder can stick to any type of surface, and is not easy to handle in granulation and tabletting process, it is more desirable to recrystallize the mefenamic acid in suc...

Acute And Sub-Chronic Toxicity Studies Of A Standardized Extract Of Centella Asiatica Eca 233, Songpol Chivapat, Pranee Chavalittumrong, Mayuree H. Tantisira

The Thai Journal of Pharmaceutical Sciences

Acute toxicity study of Centella asiatica standardized extract ECa 233 was conducted by an oral administration of 10.0 g/kg extract into 10 male and 10 female mice. The extract at the given dose did not cause any toxic signs and death within the observat...

Hptlc-Densitometric Method Development And Validation For Simultaneous Determination Of Lamivudine, Nevirapine And Zidovudine In Fixed Dose Combinations, Gebremedhin Solomon, Ariaya Hymete, Abdel-Maaboud I. Mohamed, Adnan A. Bekhit

The Thai Journal of Pharmaceutical Sciences

In this study an HPTLC-densitometric method was developed for the simultaneous determination of lamivudine, nevirapine and zidovudine in pharmaceutical dosage forms which are used widely as first line HIV/AIDS treatment. Chromatographic separation of the...

Ion-Pair Titrimetric Assays Of Quetiapine Fumarate In Pharmaceuticals Using Sodium Tetraphenylboron And Sodium Lauryl Sulphate, N. Rajendraprasad, K. Basavaiah, Kanakapura B. Vinay

The Thai Journal of Pharmaceutical Sciences

Two simple, rapid, precise and accurate titrim etric methods employing sodium tetraphenylboron (STPB) and sodium lauryl sulphate (SLS) as titrants are described for the quantitative determination of quetiapine fumarate (QTF) in bulk drug and tablets. The...

Simultaneous Determination Of Ampicillin Sodium And Sulbactam Sodium In Binary Mixtures And Commercial Dosage Forms Using Chemometrics - Assisted Spectrophotometric Techniques, Zelalem M, Abdel-Maaboud I., Adnan A. Bekhit, Ariaya Hymete

The Thai Journal of Pharmaceutical Sciences

The binary mixture of ampicillin sodium and sulbactam sodium is officially analyzed by HPLC but can not be simultaneously determined by the normal spectrophotometric techniques since their absorption spectra have a strong degree of overlapping. The curre...

Minocycline And Magnesium In Combination Attenuates Oxidative Stress And Decreases Ischemic Neuronal Damage In Vivo, Pallab Bhattacharya, Anand Kr., Sudip Paul

The Thai Journal of Pharmaceutical Sciences

A neuroprotective strategy through a combination therapy is always being superior to any other singular therapeutic interventions, as these acts through a multifaceted approach within the brain during focal cerebral ischemia. Therefore, the development o...

Use Of Bromate - Bromide Mixture As A Reagent For The Determination Of Dothiepin Hydrochloride In Pharmaceuticals, Sameer A.M. Abdulrahman, Kanakapura Basavaiah

The Thai Journal of Pharmaceutical Sciences

The present study describes one titrimetric and two spectrophotometric methods for the determination of dothiepin hydrochloride (DOTH) in bulk drug and in tablets. The methods employed bromate-bromide mixture in acid medium as an oxidimetric as well as b...

Antioxidant Properties Of Squeezed Mangosteen Juice, Monrudee Sukma, Tanasait Ngawhirunpat, Praneet Opanasopit, Pongpun Siripong

The Thai Journal of Pharmaceutical Sciences

The aim of this study was to investigate antioxidant effects of squeezed mangosteen (Garcinia mangostana) juice as preferable diet for human consumption. Mangosteen juice was prepared from the aril part. Antioxidant properties were evaluated by total ant...

Author Index, Keyword Index, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Synthesis And Antihepatotoxic Activity Of Some New Xanthones Containing 1,4-Dioxane Ring System, Shah Alam Khan, Bahar Ahmed

The Thai Journal of Pharmaceutical Sciences

This study was aimed at the synthesis of few xanthone derivatives containing 1,4-dioxane ring system. The reaction of 3,4-dihydroxy xanthone derivatives (3a-c) with ethylene dibromide and epichlorohydrin under approriate reaction conditions afforded xant...

Effect Of Ocimum Sanctum Extract On Sodium Nitrite - Induced Experimental Amnesia In Mice, Manoj Dokania, Kamal Kishore, P.K. Sharma

The Thai Journal of Pharmaceutical Sciences

The present study investigated the antiamnesic effect of the extract of Ocimum sanctum (OS) on time induced amnesia and sodium nitrite-induced amnesia in mice by using two different rodent models of memory. The aqueous extract of OS was administered to m...

Overuse Of Antibiotics In Children For Upper Respiratory Infections (Uris): A Dilemma, Malika Taufiq, Rukhsana Wamiq Zuberi

Department for Educational Development

No abstract provided.

Secreted Factors From Human Umbilical Cord Blood Cells Protect Oligodendrocytes From Ischemic Insult, Derrick Rowe

USF Tampa Graduate Theses and Dissertations

Oligodendrocytes (OL)s are the dominant cell type in the white matter and are integral for synaptic transmission essential for proper neuronal communication between brain areas. Previous studies have shown that intravenous administration of the mononuclear fraction of human umbilical cord blood (HUCB) cells in rat models of stroke reduced white matter injury, gray matter injury and behavioral deficits. Yet the mechanisms used by HUCB cells remain unknown in ischemic injury. These studies will investigate both in vitro and in vivo approaches to elucidate this mechanism in OLs. When mature primary OLs were coincubated with HUCB cells, HUCB cells secreted soluble …

Exhausted Or Unlicensed: Can Field-Of-Use Restrictions In Biotech License Agreements Still Prevent Off-Label Use Promotion After Quanta Computer?, Kristal M. Wicks

The University of New Hampshire Law Review

[Excerpt] “In the biotechnology (biotech) industry, companies must be increasingly aware of their intellectual property and how their licensing strategies can impact their rights. When licensing patented technology, it is common practice for biotech companies to include restricted field-of-use provisions in their license agreements. Such provisions permit a licensee to only use licensed technology in a defined field and restrict use or development in another field. This licensing strategy plays an important role within the biotech industry because it allows companies to more effectively control their intellectual property and to more efficiently research and develop pharmaceutical products.

A problem that …

Anticancer And Antifungal Activity Of Copper(Ii) Complexes Of Quinolin-2(1h)-One-Derived Schiff Bases, Bernadette S. Creaven, Brian Duff, Denise A. Egan, Kevin Kavanagh, Georgina Rosair, Venkat Reddy Thangella, Maureen Walsh

Articles

The condensation of substituted aromatic aldehydes with 7-amino-4-methyl-quinolin-2(1H)-one (1) has lead to the isolation of quinolin-2(1H)-one derived Schiff bases (2–14). The copper(II) complexes (2a–14a) of the ligands were also prepared, and together with their corresponding free ligands were fully characterised by elemental analyses, spectral methods (IR, ¹H and ¹³C NMR, AAS, UV–Vis), magnetic and conductance measurements. The bidentate ligands coordinated to the copper(II) ion through the deprotonated phenolic oxygen and the azomethine nitrogen of the ligands in almost all cases. X-ray crystal structures of two of the complexes, 5a and 8a, confirmed the bidentate …

Using Photoactive Vitamin Nanoparticles As Photodynamic Antimicrobial Chemotherapeutic (Pact) Agents To Treat Chronic Wounds, Rahul A. Khanke

All Capstone Projects

The objective of the proposed study is to develop new materials and methods to manage microbial growth in chronic wounds using phototherapy. Chronic wounds are considered as a worldwide health problem. The most common chronic wounds can be classified into three categories: venous ulcers, diabetic ulcers, and pressure ulcers. Venous ulcers, which usually occur in the legs, account for about 70% to 90% of chronic wounds and can become infected easily. Venous ulcers are sores that develop after veins in the legs have been damaged. These ulcers can penetrate deeply into the skin. Occasionally, if a venous ulcer persists for …