Parasitology Commons^™

Use Of Pollen Concentration In Paleopharmacology: Coprolite Evidence Of Medicinal Plants, Karl Reinhard, Donny L. Hamilton, Richard H. Hevly

Karl Reinhard Publications

Nearly 2,400 plant species have been documented ethnographically as having medicinal value among Native Americans. Therefore, it is not surprising that evidence of medicinal plants appears in some of the over 1,000 coprolites analyzed from the southwestern U.S. Three medicinal species identified in pollen analysis of coprolites are discussed. Willow (Salix) is the most common analgesic in the Native American pharmacopoeia. Prehistoric use of this plant is documented in coprolites from Bighorn Cave in the Black Mountains of Arizona and also in a burial from the Mimbres Valley, New Mexico. Historically, Mormon tea (Ephedra) served as …

Phyletic Coevolution Between Subterranean Rodents Of The Genus Ctenomys (Rodentia: Hystricognathi) And Nematodes Of The Genus Paraspidodera (Heterakoidea: Aspidoderidae) In The Neotropics: Temporal And Evolutionary Implications, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Coevolution was studied in six species of rodents of the genus Ctenomys and their parasitic nematodes of the genus Paraspidodera, collected in Bolivia. Representatives of the families Octodontidae and Caviidae were used as outgroups for the mammals, and nematodes from caviids were used as outgroups of the nematodes from ctenomyids. For the nematodes, quantitative and qualitative morphological characteristics of both males and females and electrophoretic characters of both sexes were used to generate phylogenetic hypotheses of evolutionary relationships of the OTUs occurring in hosts of different species. Concordance estimates of cladograms generated from biochemical-genetic and morphological data of the …

Review Of Les Nématodes Syphaciinae, Parasites De Rongeurs Et De Lagomorphes. Taxonomie, Zoogéographie, Évolution By Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires Du Muséum National D'Histoire Naturelle, Paris, 1988. E. J. Brill, Leiden, The Netherlands, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Les Nématodes Syphaciinae, Parasites de Rongeurs et de Lagomorphes. Taxonomie, Zoogéographie, Évolution by Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires du Muséum National d'Histoire Naturelle, Paris. 1988. 148 p. + Appendix. Available from E. J. Brill, Leiden, The Netherlands.

Effect Of Desiccation, Ph, Heat, And Ultraviolet Irradiation On Viability Of Baculovirus Penaei, Brian D. Leblanc, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Tolerance of Baculovirus penaei virus to desiccation, pH, heat, and ultraviolet irradiation (uv), as well as its survival in 32 ppt sea water, was investigated using larval Penaeus vannamei in a bioassay. Test shrimp were sampled from 36 to 264 hr after food-borne exposure to treated B. penaei and examined for patent infections (polyhedral inclusion bodies detectable in fresh preparations of hepatopancreas) with light microscopy. B. penaei was completely inactivated within 30 min after exposure to pH 3, whereas exposure to pH 11 extended the prepatency period (period before infection is apparent) but did not inactivate the virus. B. penaei …

Salsuginus Yutanensis N. Sp. (Monogenea: Ancyrocephalidae) From Fundulus Sciadicus In Clear Creek Of Eastern Nebraska, Michael T. Ferdig, Mary Ann Mcdowell, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Salsuginus yutanensis n. sp. (Monogenea: Ancyrocephalidae) is described from the gills of the Plains topminnow, Fundulus sciadicus Cope, from Clear Creek in eastern Nebraska. Salsuginus yutanensis is distinguished from previously described species by having a shorter accessory piece and different hamulus proportions, especially in the relative lengths of deep and superficial roots. In addition, the angles between deep and superficial roots distinguish S. yutanensis from several congeners.

Fixing Coccidian Oocysts Is Not An Adequate Solution To The Problem Of Preserving Protozoan Type Material, Donald W. Duszynski, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K₂Cr₂O₇. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. The parameters examined were lengths and widths of oocysts and sporocysts, percent sporulation (%S), and percent crenation (%C) of oocysts …

Heliconema Brooksi N. Sp. (Nematoda: Physalopteridae) From The Ophichthid Eel Ophichthus Gomesi In The Gulf Of Mexico, John L. Crites, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Heliconema brooksi n. sp. is described from the shrimp eel, Ophichthus gomesi, in Mississippi Sound. This species is distinguished from other members of the genus by possessing in the male 40 or more tessellated longitudinal ridges, a spicule ratio averaging 1:10.6, 4 precloacal papillae, and 6 postcloacal papillae. Females have a protruding vulvular flap. Both sexes have a prominent sclerotized cardium. Heliconema brooksi is morphologically most closely related to Heliconema heliconema. A juvenile worm from the white shrimp, Penaeus setiferus, in the same locality may be the infective stage.

Human Anisakis Transmitted By Marine Food Products: Raw Facts On Sushi And Sashimi, Thomas L. Deardorff, Stephen G. Kayes, Takakazu Fukumura

Harold W. Manter Laboratory of Parasitology: Library Materials

A seafood connection with parasitic diseases in Hawaii clearly has been established and clinicians and physicians need to become more aware of this association. The risk of people in Hawaii becoming infected with these parasites from seafood products are extremely low; however, this risk increases slightly if imported fresh fishes raw or partially cooked are eaten. Prevention of these diseases, therefore, is probably best accomplished by providing continuous, accurate, and responsible information to the public concerning the health risks of eating raw fishery products; by advising the seafood industry of potential problems and methods of addressing them properly; and by …

Early Development Of Larval Taenia Polyacantha In Experimental Intermediate Hosts, Osamu Fujita, Yuzaburo Oku, Munehiro Okamoto, Hiroshi Sato, Hong Kean Ooi, Masao Kamiya, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The early larval development and migration route of Taenia polyacantha were examined using oral inoculation of oncospheres into red-backed voles and Mongolian gerbils. The larvae were recovered in the wall of the small intestine and in the mesenteric lymph nodes by 5 days postinfection (PI) and from the peritoneal cavity after 6 days PI. These results suggest that the larval cestodes developed initially in the wall of the small intestine and the mesenteric lymph nodes, and later migrated to the peritoneal cavity. Although the development of the parasite was quite similar in the 2 host species, pathological changes were different. …

A Redescription Of Ostertagia Bison Is (Nematoda: Trichostrongyloidea) And A Key To Species Of Ostertagiinae With A Tapering Lateral Synlophe From Domestic Ruminants In North America, J. Ralph Lichtenfels, P. A. Pilitt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Ostertagia bisonis Chapin, 1925, is an abomasal worm of the American buffalo, Bison bison, and other ruminants including cattle in which it can cause clinical nematodiasis. This report describes characteristics of O. bisonis, especially details of the synlophe and esophagus, that are necessary for constructing a key to the species of medium stomach worms (Ostertagiinae) parasitic in domestic ruminants in North America. The synlophe of O. bisonis is most similar to the single ridge tapering lateral synlophe of Ostertagia ostertagi. Ducts of the subventral glands of the esophagus empty anterior to the cervical papillae and the esophageal-intestinal …

Génétique Systematique Et Des Trichinella [Table Ronde] = Genetics And Systematics Of Trichinella [Round Table], K. D. Murrell, J. Ralph Lichtenfels, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Up to the present time, the existence of two species in the genus Trichinella has been accepted by most workers. The proliferation of isolates and the definition of numerous genetic populations have caused considerable confusion in recent years for taxonomists and others concerned with these nematodes. The application of the biological species-concept to nematodes of the genus Trichinella has been difficult, but the recent investigations of Dr. E. Pozio and coworkers at the Trichinella Reference Center (Laboratory of Parasitology, Istituto Superiore di Sanita, viale Regina Elena, 299-00161 Rome, Italy) have provided new information on numerous isolates from localities worldwide. By …

Lists Of Larval Worms From Marine Invertebrates Of The Pacific Coast Of North America, Hilda Lei Ching

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Immature stages of seventy-three digenetic trematodes are listed by their families, marine invertebrate hosts, and localities and then cross listed according to their molluscan hosts. The list contains many new host records, and larval stages of fifteen digenetic trematodes were newly recorded from gastropods from British Columbia and California. A list of immature stages of fourteen cestodes, acanthocephaIans, and nematodes is also included.

Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …

Rickettsial And Mollicute Infections In Hepatopancreatic Cells Of Cultured Pacific White Shrimp (Penaeus Vannamei), Rena M. Krol, William E. Hawkins, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Infections by multiple species of bacteria occurred in hepatopancreatic epithelial cells of cultured Pacific white shrimp (Penaeus vannamei). Grossly, hepatopancreases of moribund shrimp were pale white. Light microscopically, hepatopancreatic tubules appeared atrophied and were associated with granulomas. Examination by scanning and transmission electron microscopy revealed heavy cytoplasmic infections by three forms of microorganisms: (1) a rickettsia-like bacterium, (2) a helical form of a mollicute-like bacterium, and (3) a filamentous mollicute-like bacterium. The rod-shaped rickettsia (900 nm long by 300 nm wide) appeared to be free in the cytoplasm and had both a plasma membrane and a cell wall. …

Dietary And Parasitological Analysis Of Coprolites Recovered From Mummy 5, Ventana Cave, Arizona, Karl Reinhard, Richard H. Hevly

Karl Reinhard Publications

Four coprolites were excavated with Burial 5 at Ventana Cave. a partially mummified five-year-old child. Two coprolites were granular and dark in color and two were fibrous and light in color. The coprolites are remains of the child's intestinal contents and were submitted for dietary and parasitological analysis. No parasites were found. The fibrous coprolites proved to be remains of highly masticated mesquite pods (Prosopis). The granular coprolites consist of seeds of saguaro cactus (Carnegiea gigantea). Pollen analysis reveals two dietary pollen types, both derived from cactus. No evidence of cultivated plants except for a trace amount of …

Redescription Of Eimeria Escomeli (Rastegaieff, 1930) From Myrmecophaga Tridactyla, And A First Report From Bolivia, Scott Lyell Gardner, Steve Upton, C. R. Lambert, O. C. Jordán

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Eimeria escomeli (Rastegaieff, 1930) Levine and Becker, 1933, is redescribed from the giant anteater, Myrmecophaga tridactyla (L.), from the departamento de La Paz, Bolivia. This is the first report of parasites from M. tridactyla from Bolivia and only the third time that coccidians have been recorded from this host.

A Statistical Approach To The Objective Differenciation Of Hirschmanniella Oryzae From H. Belli (Nemata : Pratylenchidae), Renaud Fortuner, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Alternate English title: A Statistical Approach to the Objective Differentiation of Hirschmanniella oryzae from H. belli (Nemata : Pratylenchidae)

English language abstract: California populations attributed to Hirschmanniella belli were compared i) to paratypes of this species, ii) to topotypes of H. oryzae, and iii) to other populations of the same genus from other parts of the world. Comparisons were made using discriminant function analyses. Some small differences between California populations and paratypes of H. belli were attributed to artifacts caused by the long storage of these paratypes. Several characters were selected that were not affected by these artifacts, but …

Long-Term Adult Population Fluctuations And Distribution Of The Spot, Leiostomus Xanthurus, In Mississippi, Brian D. Leblanc, Deborah L. Murphy, Robin M. Overstreet, Michael J. Maceina

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Adult specimens of the spot. Leiostomus xanthurus, were collected from bayou, Mississippi Sound, and barrier island locations along the Gulf Coast of Mississippi from November 1982 to July 1989. 7he mean total length of all spot sampled in comparable gill net sets was 219 mm (± 14 standard deviation, n=4,338). Ninety-five percent of the spot were collected in the island and sound areas, where the salinity was higher than in the bayous. Catch per unit effort was high at island and sound stations in spring and autumn, with relatively few fish caught during the winter spawning season and …

Plasmodium Falciparum Appears To Have Arisen As A Result Of Lateral Transfer Between Avian And Human Hosts, A. P. Waters, D. G. Higgins, T. F. Mccutchan

Harold W. Manter Laboratory of Parasitology: Library Materials

It has been proposed that the acquisition of Plasmodium falciparum by man is a relatively recent event and that the sustained presence of this disease in man is unlikely to have been possible prior to the establishment of agriculture. To establish phylogenetic relationships among the Plasmodum species and to unravel the mystery of the orign of P. fakiparum, we have analyzed and compared phylogenetically the small-subunit ribosomal RNA gene sequences of the species of malaria that infect humans as well as a number of those sequences from species that infect animals. Although this comparison confirmed the three established …

Polymorphism Of Eimerian Oocysts Can Be A Problem In Naturally Infected Hosts: An Example From Subterranean Rodents In Bolivia, Scott Lyell Gardner, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Since 1986, 364 tuco-tucos (Ctenomys spp.) representing 7 species were collected from 16 major collecting areas representing at least 4 distinct ecological habitats in Bolivia, South America. All were examined for coccidia, and 125 (34%) had oocysts in their feces including 84 of 236 (36%) Ctenomys boliviensis from tropical palm/savanna habitats; 1 of 3 (33%) C. conoveri from a chaco thorn forest; 3 of 7 (33%) C. frater from medium altitude grass habitats; and 6 of 8 (75%) C. lewisi and 31 of 35 (88%) C. opimus from high altitude/puna habitats. None of 3 C. …

Cytokeratin And Desmoplakin Analogues Within An Intracellular Parasite, Earl Weidner, Robin M. Overstreet, Bruce Tedeschi, John Fuseler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A significant amount of the total protein in the spore sacs of the microsporidian Thelohania sp. consisted of the cytoskeletal elements, cytokeratin intermediate filaments, and the desmosomal analogues. The cytokeratin and desmosomal analogues were organized as cage envelopes surrounding the spores within the spore sac stage. Thelohania sp. parasitizes the skeletal muscle of Callinectes sapidus, a crustacean that does not appear to have cytokeratins or desmosomes. Immunoprobe data indicate Thelohania sp. has a 240 kDa desmoplakin protein and 48, 51, 54 and 56 kDa cytokeratin polypeptides responsive to antibodies developed against bovine cytoskeletal counterparts. The cytoskeletal envelopes within the …

Introduction Of Robert B. Grieve As The Henry Baldwin Ward Medalist For 1990

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of the introduction of Robert B. Grieve as the American Society of Parasitologists Henry Baldwin Ward Medalist for 1990, given by Daniel R. Brooks.

New Species Of Andrya And Paranoplocephala (Cestoidea: Anoplocephalidae) From Voles And Mole-Rats In Israel And Syria, Jeanne M. Fair, Gerald D. Schmidt, Guta Wertheim

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Andrya rauschi n. sp. from Microtus guentheri differs from all other species in testes number (22-40) and distribution. Andryar auschi is superficially similar to Paranoplocephala omphalodes but differs in the development of the uterus. Paranoplocephala nevoi n. sp. from Spalax ehrenbergi, is most similar to Paranoplocephala gundii,/i> and Paranoplocephalaja nickii but differs in the number of testes (26-44). Paranoplocephala nevoi differs from the remaining species in the distribution of testes and in having unilateral genital pores.