Food Processing Commons^™

Moisture Sorption And Quality Characteristics Of Instant Rice, Anna Mays, Griffiths Atungulu

Food Science Undergraduate Honors Theses

Market demand for instant rice--a processed form of rice that is cooked and dehydrated before it is sold--has risen tremendously and become a significant component of the rice industry. Compared to freshly cooked rice, the quality of instant rice (in terms of texture, color, aroma, etc.) is much lower. There exists little information regarding how instant rice’s storage conditions affect its quality. Such information may be elucidated by studying sorption isotherms which describe the storage temperature, relative humidity, and instant rice moisture relationships.

The purpose of this study was to generate moisture sorption isotherms of instant rice for temperatures and …

Physico-Chemical, Organoleptic, And Antioxidant Properties Of Black Soybean Flour (Glycine Soja (L.) Merrit) Flakes With The Addition Of Bangle (Zingiber Purpureum Roxb.), Ika Dyah Kumalasari, Nurkhasanah Nurkhasanah, Sepitriani Sepitriani

Pharmaceutical Sciences and Research

Flakes are products that are generally made from cereals. In this study, we are interested to use black soybean (Glycine soja (L) Merrit) and bangle (Zingiber purpureum Roxb.) as flakes ingredients. Black soybeans have high antioxidant components, while bangle contains high antioxidant and fiber components, which enables it to capture free radicals. This study aimed to find the most favorable formula with good physical, chemical, and organoleptic characteristics, which also has the highest antioxidant activity. We used the Completely Randomized Design (CRD) method and the variation of formulation used was the ratio of black soybean flour to bangle …

Detection Of Aminoglycoside Antibiotic Residues In Pork By Quechers Method Combined With Sers Technique, Yang Haifan, Ding Li, Xu Miaowen, Shen Kang, Wang Xubo

Food and Machinery

Objective: To achieve rapid, quantitative and high-throughput detection of aminoglycoside antibiotic residues in pork. Methods: Square-array aligned SERS substrates based on Au nanoflowers （AuNFs） were prepared using PP synthetic paper as a substrate. Pork samples were pre-treated by QuEChERs method and subjected to SRES. Results: Using 4-mercaptobenzoic acid as the SERS probe molecule, the substrate exhibited good homogeneity, SERS enhancement effect, reproducibility and stability. the SERS signal intensities at the characteristic peaks at 475, 619 cm^-1 showed good linear relationships with the logarithms of the concentrations of gentamicin sulphate and neomycin sulphate, respectively （R² of 0.991 6 …

Structural Characteristics And Hypoglycemic Activity Of Polysaccharides From Different Parts Of Wampee, He Junye, Liu Cheng, Yu Chongyang, Sun Pengpeng, Ren Yuanyuan

Food and Machinery

Objective: This study aimed to develop antioxidant products and anti-diabetes drugs of wampee. The study aimed to research the structural characteristics, anti-oxidation and hypoglycemic properties in vitro of polysaccharides from different parts of wampee. Methods: The structural properties, in vitro antioxidant and hypoglycemic activities of polysaccharides from the fruit, peel and seeds of wampee (CWP-F, CWP-P, and CWP-S) were analyzed and compared from the aspects of chemical composition, molecular weight, monosaccharide composition, appearance, DPPH free radical scavenging rate and α-amylase inhibition rate. Results: CWP-F had the highest molecular weight and was mainly composed of galactose and arabinose, CWP-P was mainly …

Research Progress On The Mechanism Of Food-Derived Antihypertensive Peptides, Wang Baobei, Zhang Hui, Liu Yusong, Chen Hongbin, Guo Fengxian, Zheng Zongping

Food and Machinery

In this review, several mechanisms of antihypertensive peptides derived from food and its by-products were summarized. Mechanisms based on the RAAS system such as ACE/Ang Ⅱ/AT1R signaling pathways and ACE2/Ang (1-7)/MasR signaling pathways were included. Mechanisms targeting at KNOS system including PI3K/Akt/eNOS signaling pathway, PPAR-γ/caspase3/MAPK/eNOS signaling pathway and L-type Ca²⁺ channel were also discussed. In addition, the pathways of reducing blood pressure by inhibiting endothelin-converting enzyme (ECE) activity discovered in recent years were also introduced.

Effects Of Limited Enzymolysis And Glycosylation On Emulsifying Properties Of Soybean Protein Isolates, Li Lin, Sun Yixi, Qin Wen, Zhang Qing

Food and Machinery

Objective: This study aimed to develop novel soybean protein isolate （SPI）-based emulsifiers. Methods: The structure of SPI was modified by limited enzymolysis combined with glycosylation and the effects of this synergistic modification on the emulsification characteristics of SPI was studied. Results: Among the different components obtained from the SPI hydrolysate （SPIH）, the high molecular mass component （F30） exhibited the best emulsifying property. The emulsifying stability of F30-dextran conjugates obtained for 4 h was the best. Compared with SPI, SPIH and F30, the F30-dextran conjugates stabilized emulsions showed the lowest initial average particle size and the best storage stability. When the …

Study On The Degradation Of T-2 Toxin In Beer By Glow Discharge Plasma, Wang Xiaobo, Liu Jiannan, Zheng Lin, Chen Bingbing, Feng Zejun, Deng Qi

Food and Machinery

Objective: Exploring the optimal process for the degradation of T-2 toxin in beer by glow discharge plasma（GDP） and its impact on the physicochemical indicators of beer. Methods: Based on single-factor experiments, a response surface optimization experiment with four factors and three levels was conducted using the Box Behnken method to determine the optimal degradation conditions for T-2 toxin in beer. Results: When the discharge voltage was 570 V, the action time was 18 minutes, the discharge current was 99 mA, and initial concentration of T-2 toxin was 8.5 μg/mL. Under the control of these conditions, the degradation efficiency of the …

Genistein Adsorption Performance And Mechanism By Metal-Organic Frameworks Based On Triangular Aromatic Ligands, Yang Xiangyi, Cheng Yunhui, Yao Li, Xu Zhou, Xing Keyu

Food and Machinery

Objective： To improve the adsorption of genistein by zirconium-based metal-organic frameworks （MOFs） with triangle aromatic ligands. Methods： Two MOFs （MOF-808 and PCN-777） containing different sizes of triangle aromatic ligands were synthesized by hydrothermal method for adsorption of genistein. The synthesis of MOFs was determined by a series of characterization methods, and the adsorption performance was compared with that of a linear binary carboxylic acid ligand-constructed MOF （UiO-66）, to evaluate the effects of pore characteristics, hydrophobicity changes, and other factors on the adsorption performance, and to explore the adsorption mechanism by XPS analysis. Results： Compared with linear ligand-constructed UiO-66, the pore …

Effects Of Silymarin On S. Aureus Atcc25923 Biofilm And Its Virulence Factors, Wang Jiakang, Tang Haoguo, Chen Jing, Si Qihe, Shen Ruxiao

Food and Machinery

Objective: In this study, the effects of silymarin on the biofilm S. aureus ATCC25923 and its virulence factors were investigated. Methods: The inhibition and clearance of silymarin on S. aureus ATCC25923 biofilm formation were detected by crystal violet staining, the effects of silymarin on its biofilm metabolism were investigated by MTT staining, and the effects of silymarin on its coagulase expression and hemolytic activity were investigated by freeze-dried plasma and aseptic defibrinated sheep blood. In parallel, the effects of different concentrations of silymarin on its biofilm and Fourier transform were observed under an optical microscope. Results: The results showed that …

Screening And Enzymatic Characterization Study Of High-Yield Pprotease-Producing Bacterial Strains Derived From Shiping Stinky Tofu, Liu Chao, Zhao Liangzhong, Wang Yaoqiong, Li Ming, Feng Xuzhong

Food and Machinery

Objective: Obtaining hhigh-yield pprotease-pproducing bacterial resources through fermentation of advantageous protease-producing strains derived from Shiping stinky tofu. Methods: Single-factor and response surface experiments were used to optimize the culture medium and fermentation conditions. Using casein as the substrate, the effects of temperature, pH, metal ions and organic reagent on the protease activity and stability of strain JX-11 were investigated using enzymology techniques. Results: One strain of high-producing proteinase-secreting bacteria, JX-11, was isolated from Shiping stinky tofu, identified as Chryseobacterium pennipullorum. The optimal conditions for protease production by JX-11 strain were found to be temperature at 23.0 ℃, glucose content …

Potential Molecular Mechanism Of Celery Seed In Gout Treatment Of Network Pharmacology With Molecular Docking, Li Meijuan, Zhang Jun, Zhang Yunshu, Li Qianwei, Zhang Na, Liu Mengjiao, Zhang Renping

Food and Machinery

Objective: To study the molecular mechanism of celery seed inhibiting or treating gout by using network pharmacology and molecular docking. Methods: TCMSP database and the network maps were constructed with Cytoscape3.9.0 software. Gout-related targets were collected in GeneCard database and OMIM database, crossed with the main component targets of celery seed and imported into STRING database for protein-protein interaction （protein-protein interaction, PPI） analysis, core targets were obtained, core targets were introduced into DAVID, and GO function enrichment and KEGG pathway enrichment were performed in the database. Finally, AutoDock software was used to verify the key components with key targets. Results: …

Papaya Maturity Detection Based On Multi-Target Sampling And Improved Mask R-Cnn, Qi Guohong, Zhang Yunlong, Su Man

Food and Machinery

Objective: Improve the accuracy and robustness of papaya ripeness detection. Methods: A method of papaya ripeness detection based on multi-target sampling and improved Mask R-CNN was proposed. In the process of data expansion, the method introduced multi-object sampling technology to generate enhanced images from small data sets taken under controlled conditions, which was conducive to extending the proposed method to data sets with complex features of actual papaya images. The effectiveness and robustness of the proposed method were verified by means of average accuracy, accuracy, accuracy-recall curve and calculation time, and the results of papaya ripeness detection effect were compared …

Detection And Enrichment Characteristics Analysis Of Common Heavy Metal Content In Different Organs Of Hunan Lotus Seed, Dai Hui, Zhao Wenyu, Wang Jianhui, He Xiangyi, Long Xiao

Food and Machinery

Objective: The objective of this study is to investigate the heavy metal content in sediment and various organs of Hunan lotus seed in a planting area, as well as to explore the characteristics of heavy metal accumulation and transfer in Hunan lotus seed. Methods: A typical planting area in Hunan Province was selected for this research. The contents of Cd, Cr, Pb, Ni, Cu, Zn and Mn were measured using an inductively coupled plasma emission spectrometer. The bioconcentration factor （BCF） and transfer factor （TF） were used to evaluate the heavy metal accumulation and transfer ability of Hunan lotus seed. Results: …

Uncertainty Evaluation For The Determination Of Four Quinolone Residues In Honey By Liquid Chromatography-Tandem Mass Spectrometry, Yang Yun, Cao Yang, Li Jing, Zhong Feifei, Zuo He

Food and Machinery

Objective: This study aimed to evaluate the determination uncertainty of four quinolones residues in honey by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. Methods: Based on GB 31657.2—2021, a mathematical model for sources of uncertainty evaluation was established. The uncertainty of each variable which affect the result were calculated and the weight of each uncertainty component was assessed. Results: The uncertainties in the determination of enrofloxacin, sarafloxacin, ofloxacin and ciprofloxacin in honey were （4.97±0.81）, （10.13±1.58）, （7.67±1.23）, （14.97±2.25） μg/kg （P=95%，k=2） respectively. Conclusion: The main influencing factors of the determination uncertainty were preparation and dilution process of standard solution and …

Determination Of 7 Synthetic Colorants In Freshly Prepared Beverages By High Performance Liquid Chromatography Dual Wavelength Method With Automatic Solid Phase Extraction, Yao Yuyang, Xu Man, Pan Chunyan, Miao Xiong, Xue Qiang

Food and Machinery

Objective: A method for the determination of 7 synthetic colorants in freshly prepared beverages by high performance liquid chromatography dual （HPLC） wavelength method with automatic solid phase extraction was established. Methods: Extraction conditions: The sample was extracted 2 times with pure water, and then the supernatant combined after centrifugation. The pH of the supernatant was adjusted to 3~4. After activation by an automatic solid-phase extraction device, loading, rinsing, and elution of the sample were carried out on the Poly-sery synthetic colorant solid-phase extraction column. The eluent was concentrated to 200 μL by nitrogen blowing, and then fixed to 1.0 mL …

Determination The Residues Of Matrine And Oxymatrine In Liupao Tea By Uplc-Ms/Ms With Isotope Internal Standard Method, Shi Jine, Zhang Hongyu, Xin Ruozhu, Ding Mei, Xi Guangsheng

Food and Machinery

Objective: Taking Guangxi Liupao tea as an example, an UPLC-MS/MS analysis method with residual amounts of matrine and Oxymatrine was established. Methods: The prepared samples were subjected to optimized acetonitrile aqueous solution （80% acetonitrile+0.2% ammonia water） ultrasonic extraction, mobile phase: formic acid water and formic acid methanol （both 0.1%）, gradient elution, and internal standard method quantification. Results: It was linear in the range of 0.1～80.0 ng/mL, r was greater than 0.999 6, the detection limits of matrine and oxymatrine were 1.0 μg/kg, and the quantitative limits were 3.0 μg/kg, which met the regulatory determination requirements for the residue of matrine …

Analysis Of Uncertainty In Determination Of Folic Acid In Milk Powder By Microplate Method, Liu Ziyin, Zhuo Yiming, Liu Lalan, Peng Na, Wu Houlu

Food and Machinery

Objective: To analyze and evaluate the uncertainty of the determination of folic acid content in milk powder by microplate method. Methods: The content of folic acid in infant formula milk powder was determined by microplate method according to GB 5009.211—2022 "National Standard for Food Safety-Determination of Folic Acid in Foods". Various experimental factors affecting the test results were analyzed and calculated according to the uncertainty evaluation methods and guidelines such as JJF 1059.1—2012, CNAS-CL01-G003:2011 and CNAS-GL05:2011, and the synthetic standard uncertainty and expanded uncertainty were obtained. Results: The expanded uncertainty U₉₅=22.08 μg/100 g（k=2）, and the folate …

Study On The Performance Of Heat Pump Assisted Random Filler Surface Evaporation Device For Concentrated Thermosensitive Feed Liquid, Ma Yinglu, Chen Dong, Hu Shengwei, Wang Shuo, Zhang Huijuan, Xie Jihong

Food and Machinery

Objective： A surface evaporation concentration device were designed to concentrate thermosensitive feed liquid at low temperature under normal pressure with low energy consumption. Methods： Based on an introduction to the working principle of the device, the basic equations of the device were given. The changes of volumetric evaporation flux and energy saving ratio with flow rate of the feed liquid, feed liquid temperature, sweeping gas flow rate, sweeping gas temperature were calculated and analyzed. Designed an experimental platform and obtained a set of optimal performance parameters through experiments. Results： The results showed that when the flow rate of the feed …

Research On The Tooth Roll Power Of Roller Mill Based On Discrete Element, Zhao Qifeng, Wu Wenbin, Wang Xuefeng, Jia Huapo

Food and Machinery

Objective: To optimize the operating parameters of wheat milling in roller mill I skin mill to reduce the energy consumption of milling. Methods: Using EDEM discrete element simulation software, Box-Behnken test was designed for key milling factors such as roll pitch, feeding amount, rotational speed and rotational speed ratio to investigate the relationship between operating parameters and milling power. Results: The significance rankings of the effects on the toothed roll crushing power were, in order, speed ratio, roll pitch, feed volume, speed ratio quadratic term, roll pitch quadratic term, interaction of roll pitch and speed ratio, interaction of feed volume …

Robot's Automatic Strategy For Fermented Grains Scooping Based On Machine Vision, Tian Huairui, Tian Jianyan, Wang Sugang, Wang Xiaobo

Food and Machinery

Objective: To solve the problems of low level of automation and high labor intensity of workers in the fermented grains discharging process of solid-state fermentation in the ground-pot. Methods: An automatic fermented grains scooping strategy for the compound robot for fermented grains scooping was proposed. Based on the pre-processing of the ground-pot image and the reconstruction of the point cloud inside the inner edge, the point cloud segmentation algorithm was used to extract the point cloud of fermented grains. The scooping plane was constructed. The safe working distance for scooping was solved. Preset scooping points were set. Afterwards, the scooping …

Effect Of Chlorine Dioxide Fumigation Treatment On Quality Of Broccoli, Li Yuqing, Lu Lixin, Wang Qing

Food and Machinery

Objective： This study aimed to solve the problems of yellowing, discoloration and quality preservation of broccoli after harvesting. Methods： Broccoli was fumigated with chlorine dioxide gas at different mass concentrations (0,0.2, 0.5, 1.0, 1.5, 2.0 mg/L) at 23 ℃, and the sensory, color, yelination grade, physiological and biochemical indexes and related browning enzyme activities of broccoli were determined after 0,2,4,6,8 days of storage. Results： Compared with the control group, different mass concentrations of ClO₂ could inhibit the mass loss rate, yellowing browning and spoilage of flower buds of broccoli. ClO₂ at 0.5 and 1.0 mg/L had a more …

Study On The Performance Of Porcine Liver Protein Composite Membrane And Its Application In Meat Preservation, Yang Le, Liu Lili, Wang Haoyang, Chen Hui, Ding Yue

Food and Machinery

Objective: A new type of natural food preservative with pork liver as the main raw material was developed. Methods: Water-soluble porcine liver protein (WSLP), salt-soluble porcine liver protein (SSLP) and chitosan (CS) were combined to create edible composite membranes. The edible membrane components were then examined from a variety of perspectives using mechanical properties, Fourier transform infrared spectroscopy (FT-IR) and microstructure analysis. Utilized to preserve pork, and by contrasting the changes in the physical and chemical indices during the chilling process, the freshness retention capabilities of the composite film were investigated. Results: With the addition of WSLP and SSLP, the …

Effects Of Four Packaging Materials On Preservation Of Passion Flower （Passiflora Caerulea L.） Fruit, Lin Yuzhao, Chen Leiyi, Chen Hongbin, Zheng Jinshui, Jiang Xuanjing, Yang Jingmei

Food and Machinery

Objective: The preservation effects of four packaging materials on passion fruit were studied. Methods: The fruit of passion flower cv. Huangjin （maturity: eight） was used as the research material, and passion flower fruit was treated via polyvinyl chloride （PVC） film, polyethylene （PE） bag, bidirectional stretch polyolefin （BSP） heat shrinkable film, biodegradable modified atmosphere （BMA） bag, respectively, with untreated ones as the control group. Then, the fruits was stored at temperature of （25±1） ℃ and relative humidity of 85% for fifteen days, and the preservation indices of fruit were measured every three days. Results: Compared to control passion fruit, the four …

A Preliminary Study On The Effect Mechanism Of A Rice Immune Peptide In Mice, Wen Shiyu, Zhang Zhimeng, Wu Hao, Xie Yufei, Huang Qingming, Liao Juan, Wen Li

Food and Machinery

Objective: This study aimed to demonstrate the potential immunomodulatory activity in mice of rice immunopeptide GBP1 （NSVFRALPVDVVANAYR） screened previously. Methods: Animal experiments were conducted to investigate the immunomodulatory mechanism of GBP1 peptide. RNA-Seq was used to sequence the transcriptome of mouse spleen tissues before and after GBP1 peptide treatment, and the molecular mechanism of GBP1 peptide immunomodulatory effect was explained. Results: In the GBP1 peptide treatment group, the number of spleen cells tended to be normal, the liver organ coefficient decreased significantly, and the serum IgG content was stable.GBP1 peptide can affect the expression of cytokines, mediate signal transduction pathways, …

The Antitumor Activity Of Brassica Rapa L. Acid Polysaccharides In Vivo, Kadeer Gulimila, Maitiruze Amannisa, Li Mingzhu, Kang Jinsen, Taoerdahong Hailiqian

Food and Machinery

Objective: To explore the effects of Brassica rapa L. acid polysaccharide 1 (BRAP-1) on H226 cell tumor-bearing mice. Methods: 60 mice were divided into the model group (0.1 mL/10 g), positive cisplatin [3 mg/(kg·2 d)], BRAP-1 low dose [50 mg/(kg·d)], BRAP-1 medium dose [100 mg/(kg·d)] and BRAP-1 high dose [200 mg/(kg·d)]. During the experiment, the tumor growth of mice was observed, and the tumor inhibition rate and organ index were calculated; The levels of interleukin-18 (IL-18), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were detected by enzyme-linked immunosorbent assay. The gene expressions …

Extraction Of Active Ingredients From Penthorum Chinense Pursh And Comparison Of Component Distribution In Different Parts, Xiang Zhuoya, Zhu Boyu, Zhu Yongqing, Xia Chen, Chen Song

Food and Machinery

Objective: This study aimed to determine the extraction solvent and compare the distribution of functional ingredient and antioxidant activities in different parts of Penthorum chinense pursh. Methods: the total phenolic content, total flavonoid content, antioxidant capacity and individual phenolic compounds content in Penthorum chinense pursh were determined and analyzed. Results: The total phenolic content, total flavonoid content increased and then decreased with the increase of solvent concentration. The seven main phenolic compounds （catechin, rutin, pinocembrin-7-O-glucoside, quercetin, kaempferol, pinocembrin and thonningianin A）, ABTS radical scavenging ability, and ferric ion reduction ability were the highest in 80% ethanol extract. The …

Analysis Of Flavor Characteristics Of Herbal Cultivated Tremella Fuciformis Based On Component Difference Analysis And Intelligent Sensory, Chen Wanchao, Wu Di, Xiao Minsheng, Yao Shuxian, Yang Yan, Liu Yanfang

Food and Machinery

Objective: Clarify the flavor characteristics of Tremella fuciformis cultivated on herbaceous substrate. Methods: In the study T. fuciformis cultivated on herbaceous substrate was used as the research object, while samples of yellow tremella and white tremella cultivated on conventional substrate were used as controls. The differences in flavor composition between them were analyzed using high-performance liquid chromatography and Headspace Solid Phase Microextraction Ggas Chromatography Mass Spectrometry (HS-SPME-GC-MS). And combined with intelligent sensory technology, its flavor characteristics were analyzed. Results: According to multi-dimensional evaluations such as fresh and sweet amino acid and flavor nucleotides content, taste activity value and equal freshness …

Comparative Study On Several Black Bean Components, Antioxidant And Enzyme Inhibition In Vitro, Dang Yuting, Zhang Yan, Jing Boxing, Su Xiaomeng, Chai Xiyan

Food and Machinery

Objective: The difference in bioactive substance content and bioactivity in black beans were compared. Methods: The contents of total phenolic acids, total flavonoids, condensed tannins, total anthocyanins, total polysaccharides and total proteins of nine kinds of black beans were determined. The antioxidant activities of black bean extracts in vitro and their inhibitory effects on α-amylase, pancreatic lipase, acetylcholinesterase and tyrosinase were compared. Results: The contents of total phenolic acid, total flavonoids, condensed tannins and total anthocyanins were as follows: equine bean > small black bean > black bean; Total polysaccharide content: small black bean > black bean > horse bean; Total protein content: horse …

Effects Of Grape Seed Proanthocyanidins On Hepg2 Cell Genes And Related Functions Based On Transcriptome Sequencing, Zheng Wancai, Dang Bin, Zhang Ji, Luo Qiaoyu, Feng Zuoshan

Food and Machinery

Objective： Based on transcriptome sequencing analysis, the effect of grape seed procyanidins on HepG2 cell genes and related functions was identified. The key genes and metabolic pathways involved in the treatment of HepG2 cells with procyanidins were confirmed. Methods: Through transcriptome sequencing, screening differential genes, gene functional annotation and enrichment analysis of the KEGG pathway, transcriptome studies were conducted on grape proanthocyanidin-treated cells. Results: After treating HepG2 cells with grape anthocyanins, the main biological processes enriched are cellular processes, metabolic processes, biological regulatory processes, immune system processes, reproductive regulation, and growth regulation. Research has found that 12 key differentially expressed …

Optimization Of Anthocyanin Copigmentation Formula Of Purple Corn And Its Effect On The Quality Of Purple Corn, Pang Wenqian, Yu Rui, Li Dajing, Liu Chunju, Bai Bing

Food and Machinery

Objective: This study aimed to explore the copigmentation formula of purple corn anthocyanins. Methods: In this study, fructose, tannic acid, and pectin were used as co-pigmentation. The response surface method was used to optimize the co-pigmentation formula of purple corn anthocyanins, and the untreated purple corn was used as a control test. The anthocyanin components, color, texture, taste and antioxidant activity (DPPH free radical scavenging ability, ABTS free radical scavenging ability, and FRAP iron ion reducing ability) of purple corn after different treatments were determined. Results: 19% fructose, 0.07% tannic acid, and 1.9% pectin had the best copigmentation effect. Compared …