Entomology Commons^™

Investigation Of Insect-Weed Interactions In The Rice Agroecosystem, Kelly V. Tindall

LSU Doctoral Dissertations

Interactions between graminaceous weed and insect pests of rice and between management practices for these pests were investigated. Studies were conducted to examine preference and performance of rice water weevil, Lissorhoptrus oryzophilus Kuschel, on several weeds commonly found in rice fields. Several weeds were more preferred than rice, and larvae were present on several dicotyledonous weeds, suggesting the host range of L. oryzophilus is broader than previously thought. Effects of the presence of barnyardgrass, (Echinochloa crus-galli Beauv.), on rice stink bugs, Oebalus pugnax F., and L. oryzophilus populations in rice fields were investigated. Presence of barnyardgrass and the synchrony …

Insect Pest Management Approaches Among Currently Recommended Sugarcane Varieties In Louisiana, Frederick R. Posey

LSU Master's Theses

The sugarcane borer, Diatraea saccharalis (F.), (Lepidoptera: Crambidae), is responsible for more than 90% of the total insect damage to sugarcane in Louisiana. The decision to apply insecticides is complex and influenced by numerous variables. Included among these variables are insect infestation levels, varieties, weather conditions, production input levels, and environmental concerns. Predicting damage that may result from infestations occurring at a particular time of the crop production season is also important. The objective of this research was to evaluate the impact of these variables on sugarcane borer populations and subsequent yield loss. Results from a two-year survey indicated a …

Faunal Succession Of Necrophilous Insects Associated With High-Pofile Wildlife Carcasses In Louisiana, Erin Jean Grindley Watson

LSU Doctoral Dissertations

The same entomological criteria used in estimating time of death for humans are applicable for deceased wildlife. Necrophilous insects associated with animal carcasses can provide wildlife law enforcement with valuable information necessary for postmortem interval estimation, and ultimately, to incriminate poachers. The purpose of this research was twofold: to establish species composition, faunal succession patterns, and species and life stage interactions of necrophilous insects associated with three wildlife species; and to identify new methods of evaluating succession patterns using statistical measures. Twenty-one large vertebrate carcasses were monitored throughout decomposition in a mixed flatwood forest in East Baton Rouge Parish, LA …

Seasonal Occurrence Of Phyllophaga Species And Biological Studies Of Phyllophage Ephilida (Say) On Sweet Potato, Ipomoea Batatas (L) Lam, In Louisiana, Aboubacar Diagne

LSU Doctoral Dissertations

The biology of Phyllophaga ephilida (Say) was studied in Louisiana with both laboratory and field experiments from 1998 through 2003. The seasonal occurrence of common adult Phyllophaga species in sweet potato growing areas was surveyed with blacklight traps. P. ephilida and P. crinita (Burmeister) were the most common species at all locations. The attractiveness of seven methyl esters of L-isoleucine and L-valine blends to P. ephilida males was evaluated in sweet potato fields in 1999 and 2000. The 100% methyl ester of L-isoleucine was significantly more attractive than all other blend ratios that were tested. The life cycle of P. …

Additions To The Aquatic Diptera (Chaoboridae, Chironomidae, Tabanidae, Tipulidae) Fauna Of The White River National Wildlife Refuge, Arkansas, Stephen W. Chordas Iii, Patrick L. Hudson, Eric G. Chapman

Journal of the Arkansas Academy of Science

The dipteran fauna of Arkansas is generally poorly known. A previous study of the Aquatic macroinvertebrates of the White River National Wildlife Refuge, the largest refuge in Arkansas, reported only 12 diptera taxa out of 219 taxa collected (Chordas et al., 1996). Most of the dipterans from this study were identified only to the family level. The family Chironomidae is a large, diverse group and was predicted to be much more diverse in the refuge than indicated by previous studies. In this study, Chironomidae were targeted, with other aquatic or semiaquatic dipterans also retained, in collections designed to better define …

Quantitative Analysis Of Hemocyte Morphological Abnormalities Associated With Campoletis Sonorensis Parasitization, Matthew W. Turnbull, Stacy B. Martin, Bruce A. Webb

Entomology Faculty Publications

Endoparasitoids of arthropods evoke host cellular immune responses that result in hemocytic encapsulation of the endoparasitoid, unless these responses are disrupted by the parasite. Our interest has focused on mutualistic viruses found in some hymenopteran endoparasitoids that disrupt hemocyte function and prevent encapsulation. Specifically, the Campoletis sonorensis polydnavirus interacts with wasp factors to suppress immunity via expression of intracellular and secreted viral proteins. To study the roles of specific parasitization-associated factors on immunocyte morphology, fluorescence microscopy was used to visualize the actin cytoskeleton in infected and uninfected cells, or after treatment with C. sonorensis ovarian proteins or plasma from infected …

The Bacterium Xenorhabdus Nematophila Inhibits Phospholipases A2 From Insect, Prokaryote, And Vertebrate Sources, Youngjin Park, Yonggyun Kim, David Stanley

Department of Entomology: Faculty Publications

The bacterium Xenorhabdus nematophila is a virulent insect pathogen. Part of its pathogenicity is due to impairing cellular immunity by blocking biosynthesis of eicosanoids, the major recognized signal transduction system in insect cellular immunity. X. nematophila inhibits the first step in eicosanoid biosynthesis, phospholipase A₂ (PLA₂). Here we report that the bacterium inhibits PLA₂ from two insect immune tissues, hemocytes and fat body, as well as PLA_2s selected to represent a wide range of organisms, including prokaryotes, insects, reptiles, and mammals. Our finding on a bacterial in-hibitor of PLA₂ activity contributes new insight into …

Lipopolysaccharide Evokes Microaggregation Reactions In Hemocytes Isolated From Tobacco Hornworms, Manduca Sexta, Jon S. Miller, David W. Stanley

Department of Entomology: Faculty Publications

Insect cellular immune reactions to bacterial infection include nodule formation. Eicosanoids mediate several cellular actions in the nodulation process, including formation of hemocyte microaggregates, an early step. In previous work, we reported that isolated hemocytes produce and secrete eicosanoids that influence hemocyte behavior in response to bacterial challenge. We also reported that microaggregate formation in response to challenge was mediated by prostaglandins (PGs), but not by products of the lipoxygenase (LOX) pathways. In this paper we describe experiments designed to test the idea that exposing isolated hemocytes to lipopolysaccharide (LPS) evokes formation of hemocyte microaggregates and this cellular action is …

Abiotic And Biotic Factors Affecting The Distribution Of Solenopsis Invicta Buren, Brachymyrmex Sp., And Linepithema Humile (Mayr) In East Baton Rouge Parish, Louisiana, Jessica Lynn Rosson

LSU Master's Theses

Ant populations and their distribution are affected by many circumstances. Abiotic factors such as weather, water availability, and soil characteristics may determine if a habitat is suitable for ants. Other factors such as level of disturbance, available food resources, reproductive biology, and natural enemies also contribute to defining ant distribution. Three ant species in East Baton Rouge parish, Louisiana were studied to determine what factors affected their distribution by measuring abiotic and biotic factors at sampling sites throughout the parish. These factors included weather, water availability, vegetation types, level of disturbance, and soil characteristics. Factors which predict abundance of Solenopsis …

Atrazine Induction Of A Family 4 Cytochrome P450 Gene In Chironomus Tentans (Diptera: Chironomidae), Diana K. Londono, Blair D. Siegfried, Michael J. Lydy

Department of Entomology: Faculty Publications

Cytochrome P450-dependent microsomal monooxygenase (P450) activity was measured in control and atrazine-exposed third instar midge larvae, Chironomus tentans. Significantly elevated O-demethylase activity was observed in gut homogenates taken from midges exposed to atrazine concentrations from 1 to 10 ppm for 90 h. No significant induction was observed at atrazine concentrations below 1 ppm. A region of a cytochrome P450 family 4 gene was amplified and sequenced from C. tentans larvae. Alignments of inferred amino acid sequences with other insect CYP4 gene homologues indicate a high degree of similarity. Northern blot analysis employing the CYP4 gene fragment as a …

Within-Field Distribution Of The Sunflower Midge (Diptera: Cecidomyiidae), E. W. Hodgson, I. V. Macrae, Gary J. Brewer

Department of Entomology: Faculty Publications

The sunßower midge, Contarinia schulzi Gagne (Diptera: Cecidomyiidae), is a pest of cultivated sunflower (Helianthus annuus L.). Larval feeding can cause damage and yield loss to the sunflower head. Adult emergence is extended and larvae are well protected in the sunflower receptacle, making chemical control methods difficult and expensive. Sunflower midge enter sunflower fields at the edges but fieldwide distributions occur, although the dynamics are not fully understood. Two commercial fields in 1999 and one field in 2000 were systematically sampled by dividing each field into fixed sample points. Mean egg and larval densities from each sample point were …

Flight Muscle–Specific Expression Of Act88f: Gfp In Transgenic Culex Quinquefasciatus Say (Diptera: Culicidae), Margaret L. Allen, Bruce M. Christensen

Department of Entomology: Faculty Publications

A strategy to engineer a strain of Culex mosquitoes refractory to filarial transmission is described. A requirement for success of the strategy is identification of a flight muscle–specific promoter that functions in the mosquito. A GFP marker gene under the control of the promoter region of the Drosophila melanogaster act88F gene was inserted into the genome of Culex quinquefasciatus. Transformation was confirmed by Mendelian genetics. Hy-bridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles.

Supercooling Differences In The Eastern Subterranean Termite (Isoptera: Rhinotermitidae), Brian J. Cabrera, Shripat T. Kamble

Department of Entomology: Faculty Publications

Supercooling points were determined for untreated field-collected and untreated laboratory-maintained Reticulitermes flavipes (Kollar) workers and soldiers. Workers treated with antibiotics or had their hindgut-protozoa removed by exposing them to oxygen under pressure to determine the effects of absence of the hindgut fauna on supercooling. Supercooling points were compared be-tween live and freshly killed workers to determine whether supercooling in this species might be simply due to the biochemical properties of body fluids. Laboratory-maintained workers were also subjected to desiccation, starvation, or atmospheric pressure to determine their effects on supercool-ing. Supercooling points were lowest for laboratory workers treated with antibiotics and …

Molecular Differentiation Of Two Sibling Species Of The Black Fly Simulium Vittatum (Diptera: Simuliidae) Based On Random Amplified Polymorphic Dna, G. A. Duncan, P. H. Alder, K. P. Pruess, T. O. Powers

Department of Entomology: Faculty Publications

Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on pol-yacrylamide gels. The entire data set of 96 amplicons produced incomplete separation of the two cytospecies when subjected to neighbor-joining and maximum parsimony analyses. However, when analyzed within geographical regions, separate species status was supported. Bootstrap support for distinctness of the two cytospecies was best in Colorado where they were collected in true sympatry. The IS-7 cytospecies …

Impact Of Ceutorhynchus Litura Feeding On Root Carbohydrate Levels In Canada Thistle (Cirsium Arvense), Gary L. Hein, Robert G. Wilson

Department of Entomology: Faculty Publications

Canada thistle is a serious perennial weed found throughout the northern regions of the United States and Canada. The weevil, Ceutorhynchus litura (F.), was first released in Canada in 1965 as a potential biological control agent for Canada thistle; however, its impact as a control agent has been sporadic. The objective of this study was to characterize C. litura impacts on the carbohydrate profile in Canada thistle roots through the growing season and to evaluate the potential for this biological control agent in causing stress to Canada thistle. Field plots, infested with C. litura, were established and extensively sampled …

Alfalfa: Simulated Clover Leaf Weevil Injury And Alfalfa Yield And Quality, Rodney A. Madsen, Thomas E. Hunt, Leon G. Higley

Department of Entomology: Faculty Publications

The clover leaf weevil (Hypera punctata F.) is a sporadic but potentially serious pest of alfalfa (Medicago sativa L.). Feeding of newly emerged adult fourth instar clover leaf weevil was simulated in a field study at the University of Nebraska Haskell Agricultural Research Lab near Concord, Nebraska, in May 2001 and 2002. Twelve 1- by 1-m plots were arranged in a randomized complete block design with three treatments and four replications. Treatments consisted of two levels of simulated clover leaf weevil defoliation of alfalfa and an undefoliated check. Leaflets were removed over 10 d. Dry matter yield and …

Natural Distribution Of Hymenopteran Parasitoids Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae In Mexico, Jaime Molina-Ochoa, James E. Carpenter, Roberto Lezama-Gutiérrez, John E. Foster, Martín González-Ramírez, César Andres Angel-Sahagún, Javier Farías-Larios

Department of Entomology: Faculty Publications

Se llevó a cabo un inventario de parasitoides de larvas del gusano cogollero, Spodoptera frugiperda (J. E. Smith) (FAW) colectadas principalmente de maizales en estado de verticilio en seis estados mexicanos durante Agosto y Septiembre de 2000. Trece géneros de parasitoides himenópteros fueron recuperados, representando a tres familias, Braconidae: Aleoides, Chelonus, Cotesia, Glyptapanteles, Homolobus, y Meteorus); Ichneumonidae: Campoletis, Eiphosoma, Ophion, y Pristomerus; y Eulophidae: Aprostocetus, Euplectrus, y Horismenus. De un total de 5591 larvas colectadas, 772 produjeron parasitoides, para una tasa de parasitismo de 13.8%. La tasa de parasitismo más alta para una colecta simple fué …

Effect Of Environment On Resistance To The European Corn Borer (Lepidoptera: Crambidae) In Maize, David B. Willmot, Bruce E. Hibbard, Larry L. Darrah, Linda M. Pollak, Kevin Montgomery, Richard C. Pratt, Craig A. Abel, James A. Hawk, Tecle Weldekidan, John E. Foster

Department of Entomology: Faculty Publications

The European corn borer, Ostrinia nubilalis (Hu¨ bner) (Lepidoptera: Crambidae), is a major pest of maize, Zea mays L., in many temperate parts of the world. Genotype-by-environment interaction effects can make relative performance unpredictable and may hamper selection for resistance to European corn borer. The objective of this study was to determine the effect of environment on genotypic reaction to European corn borer resistance in maize. A set of 12 maize inbred lines was chosen to represent a range of European corn borer responses. Eleven testing environments ranged from Delaware, Ohio, Illinois, Iowa, Nebraska, Missouri, to Mississippi. For length of …

Phospholipase A2 In Salivary Glands Isolated From Tobacco Hornworms, Manduca Sexta, Hasan Tunaz, David W. Stanley

Department of Entomology: Faculty Publications

We describe a phospholipase A₂ (PLA₂) associated with the salivary glands of tobacco hornworms, Manduca sexta. This enzyme is able to hydrolyze arachidonic acid from the sn-2 position of PLs. Addition of the calcium chelator, EGTA, or calcium, to the Tris reaction buffer impaired the PLA₂ activity, from which we infer the enzyme requires very low concentrations of calcium or perhaps other ions for optimal activity. PLA₂ activity was sensitive to protein concentration (highest activity at 25 μg protein per μl), reaction time (optimal at 30 min), buffer pH (optimal at pH 8–10), …

An Entomopathogenic Bacterium, Xenorhabdus Nematophila, Inhibits Hemocytic Phospholipase A2 (Pla2) In Tobacco Hornworms, Manduca Sexta, Youngjin Park, Yonggyun Kim, Hasan Tunaz, David W. Stanley

Department of Entomology: Faculty Publications

The entomopathogenic bacterium, Xenorhabdus nematophila, induces immunodepression in target insects and finally leads to lethal septicemia of the infected hosts. A hypothesis has been raised that the bacteria inhibit eicosanoid-biosynthesis pathway to interrupt immune signaling of the infected hosts. Here, we show direct evidence that X. nematophila inhibits the activity of phospholipase A2 (PLA2), the initial step in the eicosanoid-biosynthesis pathway. Inhibition of PLA2 was dependent on both incubation time with X. nematophila and the bacterial concentration in in vitro PLA2 preparations of Manduca sexta hemocytes. While living bacteria inhibited PLA2 activity, heat-killed X. nematophila rather increased PLA2 activity. X. …

Acaricidal Efficacy Of Various Agents In The Treatment Of Naturally Occurring Ornithonyssus Sylviarum (Acari: Macronyssidae) Infestations Of Chickens, Maggie Phillips, T. A. Yazwinski, C. A. Tucker, Jennifer Robins, Jeremy Powell, Linda Stamps

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The northern fowl mite (NFM), Ornithonyssus sylviarum, is a commonly occurring external parasite of chickens. Primarily, caged layers have the greatest incidence of this mite, with bird unrest, unthriftiness and lowered production as some of the adverse effects of the infestation. In the current study, birds with natural NFM infestations were randomized into five treatment groups, placed in individual cages in treatment-specific batteries (all in one room), and evaluated for 28 d for infestation quantification by way of index scoring and feather digest. No treatments were 100% effective in eliminating all life stages of the mite. Tetrachlorvinphos in combination with …

Host Suitability And Thermal Requirements Of Lathromeris Ovicida Risbec (Hymenoptera: Tricliogrammatidae), An Egg Parasitoid Of Cereal Stemborers In Africa, A. Chabi-Olaye, M. K. Fiaboe, F. Schulthess

All Peer-Reviewed Publications

Factors affecting the bionomics of the trichogrammatid egg parasitoid Lathromeris ovicida and its association with the two sympatric scelionid egg parasitoids Telenomus busseolae and Telenomus isis were studied in the laboratory. The potential host species employed were the noctuids Sesamia calamistis and Sesamia botanephaga, and the pyralids Eldana saccharina and Mussidia nigrivenella. Using eggs of S. calamistis Hampson as hosts, the lower and upper thresholds for development were estimated at 14.8 and 34°C, respectively. The percent parasitism, total progeny, and sex ratio decreased gradually from 42.0 to 10.2, from 56.4 to 10.8, and from 0.74 to 0.61, respectively, with host …

Building Web-Based Interactive Keys To The Hymenopteran Families And Superfamilies, Katja Chantre Seltmann

University of Kentucky Master's Theses

Traditionally manufacturing job shops either have a process layout or a product layout. The advantages of one type of layout tend to be a disadvantage for the other. Hybrid cellular constructs represents a novel fusion of process and product layouts. In this thesis, hybrid cellular constructs specifically Hybrid Flow Shops and Reoriented andamp; Reshaped Cells are clearly described in terms of their structure, key features, and modes of operation. An engineering procedure is illustrated by cases and particular manufacturing circumstances where each concept would be most useful are identified. This thesis then defines the lean practices that are compatible with …

A New Species Of Neoscelis From Jalisco, Mexico (Coleoptera: Scarabaeidae: Cetoniinae), Alan D. Mudge, Brett C. Ratcliffe, Richard L. Westcott, Felipe A. A. Noguera

University of Nebraska State Museum: Entomology Papers

Neoscelis coracina Mudge et Ratcliffe sp. n., from Jalisco, Mexico, is described and illustrated. Intraspecific variation is discussed, and diagnostic characters separating N. coracina sp. n. from N. dohrni (Westwood, 1855) are given. The key to males of New World Goliathini is revised. Notes on habitat, behavior, and collecting N. coracina sp. n. are included. A new state record is provided for N. dohrni.

The Dynastine Scarab Beetles Of Costa Rica And Panama (Coleoptera: Scarabaeidae: Dynastinae), Brett C. Ratcliffe

Bulletin of the University of Nebraska State Museum (1924–2023)

The 157 species of dynastine scarab beetles that occur in Costa Rica and Panama are comprehensively reviewed. Keys, descriptions, distributions, and notes on biology are provided for all species as well as illustrations and maps. The larvae of Enema endymion, Heterogomphus chevrolati, and Dynastes hercules are described for the first time. Also included are synopses of the higher-level taxa, a glossary, a gazetteer of place names, and a species checklist.

The following new species are described: Cyclocephala alazona, C. enigma, C. labidion, C. marylizae, C. mustacha, C. stockwelli, C. una mas (all Cyclocephalini); Irazua dilicra (Oryctini); Amblyoproctus centroamericanus, Hemiphileurus curoei, …

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Chinch Bug-Resistant Buffalograss: An Investigation Of Tolerance, Antixenosis, And Antibiosis, Tiffany Heng-Moss, Frederick P. Baxendale, Terrance P. Riordan, Linda J. Young, Kit Lee

Department of Entomology: Faculty Publications

Choice and no-choice studies were conducted to determine the categories (antibiosis, antixenosis, and tolerance) of resistance of four buffalograsses (NE91-118, ‘Bonnie Brae’, ‘Cody’, and ‘Tatanka’) previously identified as resistant to the western chinch bug, Blissus occiduus Barber. Antibiosis studies found no significant differences in western chinch bug fecundity, nymphal development, or survival among the resistant and susceptible buffalograsses. Tolerance studies indicated that NE91-118, Cody, and Tatanka exhibited moderate-to-high levels of tolerance based on western chinch bug damage ratings and plant height, whereas Bonnie Brae exhibited moderate-to-low levels of tolerance. Choice studies indicated the presence of antixenosis in NE91-118, whereas Cody …

Characterization Of General Esterases From Methyl Parathion-Resistant And -Susceptible Populations Of Western Corn Rootworm (Coleoptera: Chrysomelidae), Xuguo Zhou, Michael E. Scharf, Lance J. Meinke, Laurence D. Chandler, Blair D. Siegfried

Department of Entomology: Faculty Publications

A consistent correlation between elevated esterase activity and methyl parathion resistance among Nebraska western corn rootworm, Diabrotica virgifera virgifera LeConte, populations has previously been documented. Characterization of general esterase activity using naphtholic esters as model substrates indicated that differences between resistant and susceptible strains could be maximized by optimizing assay conditions. The optimal conditions identified here were similar to those reported for other insect species. The majority of general esterase activity was found in the cytosolic fractions of resistant populations, whereas the activity was more evenly distributed between cytosolic and mitochondrial/nuclear fractions in the susceptible population. General esterase activity was …

In-Field Labeling Of Western Corn Rootworm Adults (Coleoptera: Chrysomelidae) With Rubidium, Timothy M. Nowatzki, Bradley Niimi, Kelli J. Warren, Sean Putnam, Lance J. Meinke, David C. Gosselin, F. Edwin Harvey, Thomas E. Hunt, Blair D. Siegfried

Department of Entomology: Faculty Publications

Field and laboratory studies were conducted in 2000 and 2001 to determine the feasibility of mass marking western corn rootworm adults, Diabrotica virgifera virgifera LeConte, with RbCl in the field. Results showed that application of rubidium (Rb) in solution to both the soil (1 g Rb/plant) and whorl (1 g Rb/plant) of corn plants was optimal for labeling western corn rootworm adults during larval development. Development of larvae on Rb-enriched corn with this technique did not significantly influence adult dry weight or survival. Rb was also highly mobile in the plant. Application of Rb to both the soil and the …

A Case For Taxonomic Recognition Of The Taxon Enodia Anthedon Borealis A. H. Clark (Satyridae), Alex Grkovich, Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

Clark (1936) described the taxa anthedon and borealis both as new subspecies of Enodia portlandia (Fabricius, 1781). Clark described borealis as the northernmost phenotypically different taxon in this group. Anthedon later became recognized as a distinct species with borealis as a subspecies of it. Masters (1971) characterized E. anthedon borealis as displaying several subtle phenotypic differences from nominotypical anthedon and also noted significant differences in behavior and habitat and reinforced the continued recognition of borealis as a valid subspecies. However, a number of publications after 1971 generally failed to recognize subspecific status for borealis, either ignoring borealis entirely …