Entomology Commons^™

Molecular Characterization And Tissue-Specific Gene Expression Of Dermacentor Variabilis Α-Catenin In Response To Rickettsial Infection, Piyanate Sunyakumthorn, Natthida Petchampai, Michael T. Kearney, Daniel E. Sonenshine, Kevin R. Macaluso

Biological Sciences Faculty Publications

Alpha catenin is a cytoskeleton protein that acts as a regulator of actin rearrangement by forming an E-cadherin adhesion complex. In Dermacentor variabilis, a putative α-catenin (Dvα-catenin) was previously identified as differentially regulated in ovaries of ticks chronically infected with Rickettsia montanensis. To begin characterizing the role(s) of Dvα-catenin during rickettsial infection, the full-length Dvα-catenin cDNA was cloned and analysed. Comparative sequence analysis demonstrates a 3069-bp cDNA with a 2718-bp open reading frame with a sequence similar to Ixodes scapularis α-catenin. A portion of Dvα-catenin is homologous to the vinculin-conserved domain containing a putative …

Externally-Expressed Fluorescence Across Sexes, Life Stages, And Species Of Spiders, Erin Brandt

Dissertations and Theses

Although all spiders possess fluorophores in their hemolymph, the expression of external fluorescence is much more restricted. The purpose of this study was to evaluate differences in externally-expressed fluorescence between sexes, life stages, and species of spiders. To approach this question, we developed novel instrumentation to capture fluorescence with photographs of our specimens. We paired these fluorescence measurements with spectrometer measurements to attempt to determine the role that fluorescence plays in the overall coloration in spiders. The study was divided into four sections. First, we examined how fluorescence varies in sexes and life stages in Misumena vatia, an ambush predator …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Effects Of Methoprene On The Survivorship Of Adult Aedes Mosquitoes: A Strategy Or Inactivating Released Mosquitoes, Peter J. Brabant Iii

Theses and Dissertations--Entomology

Methoprene is a Juvenile Hormone (JH) analogue commonly used for the control of mosquito larvae. When applied to a mosquito breeding site, methoprene enters the haemolymph, where it mimics the function of JH and interferes with normal metamorphosis, resulting in larval mortality. Methoprene is commonly used for the control of larvae and has not been used as an adulticide, due to an absence of acute effects. This study evaluated possible chronic effects caused by the exposure of adult Aedes mosquitoes to methoprene. Methoprene was applied, in both technical grade and the commercially available Altosid®, topically to adults through droplet application …

Invertebrate Abundances And Diversity Of A Six Year Old Organic Apple Orchard In Northwest Arkansas, Cory Johnson, Brina Smith, Mary C. Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Ground cover mulch applications in perennial systems can have multiple benefits, one of which may be to enhance the size and diversity of the ground surface faunal community. To determine if ground cover and organic fertilizer applications altered invertebrate communities, litters in an experimental 0.4-ha organic apple orchard in Fayetteville, Ark. were sampled during a four week period beginning in February 2012. The orchard was planted in 2006 in a replicated 4 × 3 factorial design with organic ground cover and fertilizer treatments applied annually each April. Invertebrates were extracted using Berlese funnels and hand sorting techniques. Ground covers (wood …

Integrated Management Of The Red Imported Fire Ant In Pastures, William Sullivan Hilbun

LSU Master's Theses

The effects and interactions of chemical, cultural, and biological control of red imported fire ant (RIFA) populations were evaluated in two replicated studies in pastures. The first study was conducted from 2005 through 2007 in Washington parish, Louisiana (WP) where the biological control Psuedacteon tricupsis phorid flies were present and at the LSU AgCenter St. Gabriel Research Station in St. Gabriel, Louisiana (SG) where Psuedacteon tricuspis flies were absent. The second study was conducted from May 2009 to May 2010 at St. Gabriel in the presence of phorid flies. In both studies, RIFA population indices in sites treated with methoprene …

The Effects Of Spinosad On Culex Quinquefasciatus (Diptera: Culicidae) And Non-Target Insect Species, Owen Mcbride Jones

LSU Master's Theses

Spinosad is a relatively new insecticide with a unique mode of action that is being evaluated for control of larval mosquitoes. Whereas a number of toxicological studies have measured effects of spinosad on various mammals, fish, birds, and terrestrial arthropods, fewer studies have been conducted on the effects of spinosad on non-target aquatic insect species. Such studies are important as these species might be found in the same environments as mosquito larvae targeted for control. A neighborhood pond was surveyed to find a representative species of mosquito as well as other common aquatic insects with which to examine susceptibility to …

Following The Trail Of Ants: An Examination Of The Work Of E.O. Wilson, Samantha Kee

Writing Across the Curriculum

No abstract provided.

Assessment Of Stink Bug Feeding Damage In Louisiana Soybean: Use Of A No-Choice Feeding Field Protocol, Jessica Leigh Parker

LSU Master's Theses

Numerous arthropods are pests of soybean in the subtropical environment of Louisiana. The most important pod-feeding guild includes a complex of stink bugs (Hemiptera: Pentatomidae). One of these species, redbanded stink bug (Piezodorus guildinii [Westwood]), has emerged as a significant pest in Louisiana soybean. Currently, limited information is available describing soybean plant injury from this pest in the United States or compares injury relative to native stink bugs. Therefore, a field protocol was modified to assess injury to soybean seeds. Studies conducted in Louisiana during 2009-2011 used procedures to isolate the effects of infestation duration (24-96 h after infestation [HAI]) …

Evaluating The Efficacy Of Methoxyfenozide On Louisiana, Texas And The Mid-Southern Soybean Looper Populations, Sebe Anthony Brown

LSU Master's Theses

The soybean looper, Chrysodeixis includens (Walker), is a defoliating insect pest of soybean in the Mid-South and Texas. In 2008, Louisiana producers reported unsatisfactory control of soybean loopers in soybean with methoxyfenozide. In 2009 and 2010, field collections from Louisiana, Missouri, Mississippi and Texas were exposed to discriminating concentrations (0.020 to 5.000 ai ìg/ml) of methoxyfenozide in diet incorporation bioassays. All field colonies were compared to a reference strain LC50 of 0.007 ìg/ml for 2009 and 0.008 ìg/ml for 2010. Louisiana populations exhibited LC50’s of 0.079 ìg/ml and 0.122 ìg/ml for 2009 and 2010, respectively, which were the highest values …

Incorporating Neonicotinoid And Anthranilic Diamide Insecticides Into Integrated Pest Management Of Rice Water Weevil, Lissorhoptrus Oryzophilus, Srinivas K. Lanka

LSU Doctoral Dissertations

Seed treatments with chlorantraniliprole and thiamethoxam have been used for the management of rice water weevil, Lissorhoptrus oryzophilus in the United States. Both insecticides being plant-systemic could potentially target multiple life stages of L. oryzophilus. The rational use of these chemicals as seed treatments in weevil management requires thorough understanding on targeted life stages of L.oryzophilus and, the relative persistence and potency of both chemicals. A series of greenhouse and laboratory experiments were conducted on weevils by culturing rice plants treated as seeds with different rates of chlorantraniliprole and thiamethoxam. Adult feeding assays on foliage from treated plants revealed divergent …

New Geographic Distribution Records For Centipedes (Chilopoda: Scolopendromorpha) In Arkansas, Including The First From Crowley's Ridge And The Grand Prairie, C. T. Mcallister, H. W. Robison, L. C. Thompson, M. B. Connior

Journal of the Arkansas Academy of Science

No abstract provided.

Gene Expression Profiling In The Thiamethoxam Resistant And Susceptible B-Biotype Sweetpotato Whitefly, Bemisia Tabaci, Wen Xie, Xin Yang, Shao-Ii Wang, Qing-Jun Wu, Ni-Na Yang, Ru-Mei Li, Xiao-Guo Jiao, Hui-Peng Pan, Bai-Ming Liu, Yun-Tao Feng, Bao-Yun Xu, Xuguo Zhou, You-Jun Zhang

Entomology Faculty Publications

Thiamethoxam has been used as a major insecticide to control the B-biotype sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Due to its excessive use, a high level of resistance to thiamethoxam has developed worldwide over the past several years. To better understand the molecular mechanisms underlying this resistance in B. tabaci, gene profiles between the thiamethoxam-resistant and thiamethoxam-susceptible strains were investigated using the suppression subtractive hybridization (SSH) library approach. A total of 72 and 52 upand down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These expressed sequence tags (ESTs) belong to several functional categories based …

Enabling Learning And Enhancing Education In Ipm In The Midst Of Dwindling Financial Resources: Why Open Access To Resources And Publishing Could Be A Strategic Step To Support Students Today And Tomorrow?, Ousmane Youm

Department of Entomology: Faculty Publications

When I was invited to join the editorial board of the journal Entomology, Ornithology & Herpetology (EOH), I read carefully the note coming with the invitation and also looked at the content and scope. Then two things came into my mind as I stopped a few minutes to reflect on a couple of issues: (1) how the new journal links and is relevant to Integrated Pest Management—IPM (for those who understand its definition)—and (2) the issue of open access and relevance in the current situation of an ailing global economy, particularly in the vulnerable developing nations.

In the latter, more …

A Review Of The Distribution Of Harposcelis Paradoxus Burmeister, 1847 (Coleoptera: Scarabaeidae: Dynastinae: Cyclocephalini) With A New Country Record For Peru, Jochen-P. Saltin, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

Harposcelis paradoxus Burmeister is a relatively rare lowland species that, for a long time, was known only from French Guiana (Blackwelder 1944; Endrödi 1966, 1985; Krajcik 2005). Males are more commonly collected than females and are now commonly available via the Internet from commercial dealers in the insect trade. At a body length of 40 mm, this is a remarkably large species for a member of the tribe Cyclocephalini. Males are distinctive because of their long, curved protibiae and anterior trochanter with a large, anteriorly projecting tooth. The genus can be identified in keys by Endrödi (1966, 1985), Ratcliffe (1985), …

Breeding Biology And Bee Guild Of Douglas’ Dustymaiden, Chaenactis Douglasii (Asteraceae, Helenieae), James H. Cane, Byron Love, Katharine A. Swoboda-Bhattarai

Department of Entomology: Faculty Publications

Douglas’ dustymaiden, Chaenactis douglasii (Hook.) Hook. & Arn., is a widespread, inconspicuous, short-lived perennial wildflower that blooms in early summer and is found in basin sagesteppe to upper montane areas throughout the U.S. Intermountain West. The species is proving practical to grow for seed and is expected to be used for western rangeland rehabilitation. Through manual pollination experiments, C. douglasii was found to be only weakly self-fertile; 15% of flowers from geitonogamy and autogamy treatments yielded filled achenes. In contrast, 57% of outcrossed flowers produced achenes filled with endosperm, with every capitulum yielding some fertile seeds. Freely visited flowers from …

Arthur F. Hagen Obituary, Robert J. Wright

Department of Entomology: Faculty Publications

Arthur F. Hagen, 84, died Thursday, 26 July 2012 at the Community Hospital in Torrington, Wyoming.

Art was born 4 February 1928 in Huron, South Dakota, the son of Merle and Leona (Geuther) Hagen. He grew up and received his early education in Huron and Armour, SD. Art entered the U.S. Navy, where he spent four years as an aviation electrician and was discharged in 1949. He married Marlene Halstead on 23 March 1954 in Wagner, SD. Art attended college at Colorado Agricultural and Mechanical College (now Colorado State University) where he received his B.S. in 1954 and his master’s …

The European Corn Borer And Bt Corn In The United States, Blair D. Siegfried, Richard L. Hellmich

Department of Entomology: Faculty Publications

The European corn borer, Ostrinia nubilalis Hübner (Lepidoptera: Crambidae) has been a major pest of corn and other crops in North America since its accidental introduction nearly a hundred years ago. Wide adoption of transgenic corn hybrids that express toxins from Bacillus thuringiensis, referred to as Bt corn, has suppressed corn borer populations and reduced the pest status of this insect in parts of the Corn Belt. Continued suppression of this pest, however, will depend on managing potential resistance to Bt corn, currently through the high-dose refuge (HDR) strategy. In this review, we describe what has been learned with …

A Molecular Key For The Identification Of Blow Flies In Southeastern Nebraska, Upeka Samarakoon, Steven R. Skoda, Frederick P. Baxendale, John E. Foster

Department of Entomology: Faculty Publications

Immature blow flies (Calliphoridae) are typically the first colonizers of cadavers. Identification of the early instars using traditional, morphology-based keys is difficult because of their small size, similarity, and simplicity in external morphology. Information derived from molecular genetic data would augment the accurate identification of immature flies. Nine species of blow flies commonly found in southeastern Nebraska were used to examine the utility of molecular-based keys. Polymerase chain reaction–restriction fragment length polymorphisms (PCR–RFLP) were investigated with 10 common, inexpensive, restriction enzymes from an amplicon of approximately 1500 bp spanning the mitochondrial cytochrome oxidase I gene. A simple molecular taxonomic key, …

The World’S Deepest Subterranean Community - Krubera-Voronja Cave (Western Caucasus), Alberto Sendra, Ana Sofia P.S. Reboleira

International Journal of Speleology

Subsurface biota extends over a wide variety of habitats that can be spatially interconnected. The largest communities of this subsurface biota inhabit cavities and are well known mainly in caves where biologists are able to have access. Data about deep subterranean communities and arthropods living under one thousand meters was unknown.

An expedition to world’s deepest cave, Krubera-Voronja in Western Caucasus, revealed an interesting subterranean community, living below 2000 meters and represented by more than 12 species of arthropods, including several new species for science. This deep cave biota is composed of troglobionts and also epigean species, that can penetrate …

Management In A Neotropical Show Cave: Planning For Invertebrates Conservation, Thais Giovannini Pellegrini, Rodrigo Lopes Ferreira

International Journal of Speleology

Lapa Nova is a dolomitic cave about 4.5 km long located in northwestern Minas Gerais state, Brazil. The cave experiences intense tourism, concentrated over a single period of the year, during the Feast of Our Lady of Lapa. In order to evaluate the impacts felt by the invertebrate community from this tourism, a new methodology was proposed. Four types of areas (intense visitation area, outlying visitation areas, moderate visitation areas and no-visitation areas) were sampled for invertebrates. There was one sampling prior and another on the last day of the 128^th feast, to evaluate the effects of visitation on …

Diversity And Community Assembly Patterns Of Epigean Vs. Troglobiont Spiders In The Iberian Peninsula, Pedro Cardoso

International Journal of Speleology

Cave-obligate organisms usually have smaller ranges and their assemblages have higher beta diversity than their epigean counterparts. Phylogenetic and functional diversity is usually low in cave communities, leading to taxonomic and functional disharmony, with entire groups missing from the subterranean realm. The objective of this work is to compare range, beta diversity, phylogenetic and functional diversity, taxonomic and functional disharmony of epigean versus troglobiont spiders in the Iberian Peninsula.

The median extent of occurrence was found to be 33 times higher for epigean than for cave species. Beta diversity was significantly higher for troglobiont assemblages. Cave assemblages present lower phylogenetic …

Evaluation Of Bacillus Thuringiensis Corn Containing Pyramided Traits For Management Of Sugarcane Borer, Diatraea Saccharalis (F.), David Sindani Wangila

LSU Master's Theses

The sugarcane borer, Diatraea saccharalis (F.), is a major target of Bacillus thuringiensis (Bt) corn in the U.S. mid southern region. Corn expressing pyramided Bt proteins has recently become commercially available in the U.S. The objectives of this study were 1) to determine survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and –heterozygous (Cry1Ab-RS) genotypes of D. saccharalis on Bt corn containing single and pyramided Bt genes and 2) to assess larval movement of D. saccharalis in different planting patterns of non-Bt and Bt corn. One laboratory leaf tissue bioassay and two independent greenhouse trials were conducted to evaluate …

Oil-Mediated Mortality And Induced Behavioral Modifications Of Coastal Insects, Benjamin Jacob Adams

LSU Master's Theses

The Deepwater Horizon oil spill affected over one thousand kilometers of the southeastern United States’ coast in the Gulf of Mexico especially Louisiana’s salt marshes. These marshes are a vital part of the state’s economy and coastal ecology; however, the insects residing in this area remain largely unstudied. The goal of my project was to answer specific questions arising from two ongoing investigations of the insects in the oiled marshes. I sampled insects using sweep nets and insect vacuums to determine the most efficient technique to use in the marsh, compared insects collected in oiled and non-oiled marshes, and completed …

Empirical And Conceptual Approaches To Life-History Trade-Offs: The Size And Number Of Offspring In Broods Of A Parasitoid Wasp, Yoriko Saeki

Theses and Dissertations--Biology

Trade-offs in resource allocation underlie key life history traits of organisms. My dissertation focuses on the size-number trade-off in clonal broods of offspring using the polyembryonic wasp, Copidosoma bakeri parasitizing immature stages of the moth Agrotis ipsilon. I aim to characterize responses of wasp brood size and individual body mass by manipulating the environments in order to understand the allocation pattern in the size number trade-off. In reviewing the functional forms of trade-off relationships in relation to resource constraints, I distinguish among three main trade-off types based on graphical representations of the relationship between the trade-off variables: linear, convex …

Nepetalactones From Essential Oil Of Nepeta Cataria Represent A Stable Fly Feeding And Oviposition Repellent, J. J. Zhu, Dennis R. Berkebile, C. E. Dunlap, A. Zhang, D. Boxler, K. Tangtrakulwanich, R. Behle, Frederick P. Baxendale, Gary J. Brewer

Department of Entomology: Faculty Publications

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is one of the most serious pests to livestock. It feeds mainly on cattle and causes significant economic losses in the cattle industry. Standard stable fly control involving insecticides and sanitation is usually costly and often has limited effectiveness. As we continue to evaluate and develop safer fly control strategies, the present study reports on the effectiveness of catnip (Nepeta cataria L.) oil and its constituent compounds, nepetalactones, as stable fly repellents. The essential oil of catnip reduced the feeding of stable flies by >96% in an in vitro bioassay system, …

Ipm Team Representatives, Clyde L. Ogg, Erin Bauer

Department of Entomology: Faculty Publications

Contents

Acknowledgments...................... iv
Preface...................... v
Introduction...................... 1
Chapter 1 – What is Integrated Pest Management...................... 3
Chapter 2 – Monitoring and Inspecting...................... 15
Chapter 3 – Injury and Action Levels...................... 37
Chapter 4 – Treatment Strategies...................... 41
Chapter 5 – How to Develop an IPM Program...................... 49
Chapter 6 – IPM for Ants...................... 59
Chapter 7 – IPM for Cockroaches...................... 69
Chapter 8 – IPM for Bed Bugs...................... 81
Chapter 9 – IPM for Fabric and Pantry Pests...................... 87
Chapter 10 – IPM for Fleas...................... 95
Chapter 11 – IPM for Flies...................... 101
Chapter 12 – IPM for Head Lice...................... …

Using Web-Based Key Character And Classification Instruction For Teaching Undergraduate Students Insect Identification, Douglas A. Golick, Tiffany M. Heng-Moss, Allen L. Steckelberg, David W. Brooks, Leon G. Higley, David Fowler

Department of Entomology: Faculty Publications

The purpose of the study was to determine whether undergraduate students receiving web-based instruction based on traditional, key character, or classification instruction differed in their performance of insect identification tasks. All groups showed a significant improvement in insect identifications on pre- and post-two-dimensional picture specimen quizzes. The study also determined student performance on insect identification tasks was not as good as for family-level identification as compared to broader insect orders and arthropod classification identification tasks. Finally, students erred significantly more by misidentification than misspelling specimen names on prepared specimen quizzes. Results of this study support that short web-based insect identification …

Inter- And Intraspecific Identification Of The Screwworm, Cochliomyia Hominivorax, Using Random Amplified Polymorphic Dna-Polymerase Chain Reaction, Steven R. Skoda, James L. Figarola, Saowaluck Pornkulwat, John E. Foster

Department of Entomology: Faculty Publications

The screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), is one of the most devastating arthropod pests of livestock in the Western Hemisphere. Early instars are very difficult to distinguish morphologically from several closely related blow fly species. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were developed for identifying C. hominivorax from other wound inhabiting species. Forty decameric primers were screened; nine showed clear reproducible RAPD profiles suitable for distinguishing all life stages of C. hominivorax from 7 other species, including C. macellaria (Fabricius). The results from RAPD-PCR with field-collected samples of unknown first instars agreed with morphological identification that the …