Entomology Commons^™

Factors Limiting The Spread Of The Protective Symbiont Hamiltonella Defensa In Aphis Craccivora Aphids, Hannah R. Dykstra, Stephanie R. Weldon, Adam J. Martinez, Jennifer A. White, Keith R. Hopper, George E. Heimpel, Mark K. Asplen, Kerry M. Oliver

Entomology Faculty Publications

Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbiont occurs only at intermediate frequencies in field populations. To identify factors constraining H. defensa invasion in Ap. craccivora, we estimated symbiont transmission rates, performed fitness assays, and measured infection dynamics in population cages to evaluate effects of infection. Similar to results with the pea aphid, Acyrthosiphon pisum, we found no consistent costs to infection using …

Life History Of Paracoccus Marginatus (Hemiptera: Pseudococcidae) On Four Host Plant Species Under Laboratory Conditions, Kaushalya G. Amarasekare, Catharine M. Mannion, Lance S. Osborne, Nancy D. Epsky

Agricultural and Environmental Sciences Faculty Research

Life history of the mealybug, Paracoccus marginatus Williams and Granara de Willink, on three ornamental plants [Hibiscus rosa-sinensis L., Acalypha wilkesiana (Muell.-Arg.), and Plumeria rubra L.] and one weed species (Parthenium hysterophorus L.) was studied under laboratory conditions. Mealybugs were able to develop, survive, and reproduce on all four hosts; however, there were differences in the life history parameters. Adult females that developed on acalypha and parthenium emerged ≈1 d earlier than those that developed on hibiscus and plumeria. Adult males had a longer developmental time on plumeria than on the other hosts. Survival of first- and second-instar …

Sma-Causing Missense Mutations In Survival Motor Neuron (Smn) Display A Wide Range Of Phenotypes When Modeled In Drosophila, Kavita Praveen, Ying Wen, Kelsey M. Gray, John J. Noto, Akash R. Patlolla, Gregory D. Van Duyne, A. Gregory Matera

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Mutations in the human survival motor neuron 1 (SMN) gene are the primary cause of spinal muscular atrophy (SMA), a devastating neuromuscular disorder. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome. Additional tissue-specific and global functions have been ascribed to SMN; however, their relevance to SMA pathology is poorly understood and controversial. Using Drosophila as a model system, we created an allelic series of twelve Smn missense mutations, originally identified in human SMA patients. We show that animals expressing these SMA-causing mutations display a broad range of …

Molecular Xenomonitoring Using Mosquitoes To Map Lymphatic Filariasis After Mass Drug Administration In American Samoa, Mark A. Schmaedick, Amanda L. Koppel, Nils Pilotte, Melissa Torres, Steven A. Williams, Stephen L. Dobson, Patrick J. Lammie, Kimberly Y. Won

Entomology Faculty Publications

BACKGROUND: Mass drug administration (MDA) programs have dramatically reduced lymphatic filariasis (LF) incidence in many areas around the globe, including American Samoa. As infection rates decline and MDA programs end, efficient and sensitive methods for detecting infections are needed to monitor for recrudescence. Molecular methods, collectively termed 'molecular xenomonitoring,' can identify parasite DNA or RNA in human blood-feeding mosquitoes. We tested mosquitoes trapped throughout the inhabited islands of American Samoa to identify areas of possible continuing LF transmission after completion of MDA.

METHODOLOGY/PRINCIPLE FINDINGS: Mosquitoes were collected using BG Sentinel traps from most of the villages on American Samoa's largest …

Nebline, August 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Cherry Creek Habitat Provides Opportunities for Pollinator Education

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special pullout section: Lancaster County Super Fair 2014 Schedule & Map

Hexapod Herald - Vol. 26, No. 4, August 2014

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Faculty News - Publications - Grants - Travel - Meet an Off-Campus Student - Student News

An Evaluation Of Watermelon (Citrullus Spp.) Germplasm For Additional Sources Of Resistance To The Twospotted Spider Mite (Tetranychus Urticae Koch)., Hector Cantu Jr.

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fourteen U.S plant introduction (PI) accessions of C. lanatus var. lanatus (4), C. lanatus var. citroides (5) C. colocynthis (5) and a known susceptible commercial cultivar ‘Sugar Baby’ were evaluated for resistance to the twospotted spider mite, Tetranychus urticae Koch, in a limited free-choice and free-choice bioassay under laboratory conditions. The limited free choice bioassay, involved nine Petri dish cages that held five randomly assigned leaves individually inoculated with two adult females and one adult male. Eggs, larva, and adults were counted over a nine day period. The free choice bioassay involved the even distribution of three mite infested …

Host Specificity And Ectoparasite Load Of Bat Flies In Utila, Honduras, Courtney Miller

Senior Honors Theses

Bat flies (Streblidae) are obligate blood-feeding ectoparasites of bats that display varying degrees of host specificity. A total of 265 streblid bat flies were collected from 122 bats belonging to the families Phyllostomidae and Natalidae from Utila, the smallest bay island of Honduras. Out of four host-parasite associations, three were considered primary. Out of the three bat species analyzed, one had significantly lower parasite prevalence and another had significantly higher parasite load and intensity. Both male and female bats were equally likely to be infested and variables of parasite density did not differ amongst host sex for any species. However, …

Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates

Department of Entomology: Dissertations, Theses, and Student Research

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs delivery.

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is a pest of the plant family Solanaceae. RNAi in the CPB is of interest for potential use in insect management and as a tool to study the interaction with host plants. …

Characterization Of Greenbug Feeding Behavior And Aphid (Hemiptera: Aphididae) Host Preference In Relation To Resistant And Susceptible Tetraploid Switchgrass Populations, Kyle G. Koch, Nathan Palmer, Mitch Stamm, Jeff D. Bradshaw, Erin Blankenship, Lisa M. Baird, Gautam Sarath, Tiffany M. Heng-Moss

Department of Entomology: Faculty Publications

Two choice studies were performed to evaluate greenbug, Schizaphis graminum (Rondani), and yellow sugarcane aphid, Sipha flava (Forbes), preference for two tetraploid switchgrass populations, Summer and Kanlow, and one experimental hybrid, K×S, derived by crossing Kanlow (male)×Summer (female) plants. Additionally, an assessment of S. graminum feeding behavior was performed on the same switchgrass populations, by using the electrical penetration graph (EPG) technique. Choice studies for S. flava indicated a lack of antixenosis, with no preference by aphids among any of the switchgrass populations at any time point. However, choice studies with S. graminum indicated a preference for the K×S plants …

Potential Interactions Of Early Season Herbicides And Insecticides In Cotton: Thrips Control And Plant Health, Derek Lane Clarkson

Graduate Theses and Dissertations

Cotton growth early in the season is affected by damaging levels of thrips in many production fields in Arkansas. During this time, insecticides used to control thrips and herbicides used to control weeds are often present at the same time on the cotton plant. This research explores how various combinations of herbicides and insecticides influence thrips numbers and cotton plant growth parameters early in the growing season. Pathways evaluated included interactions between preemergence herbicides and insecticide seed treatments and interactions between common tank-mixed foliar herbicides and selected foliar insecticides. No interactions in thrips control or plant growth were observed when …

Nitrogen Management Practices And Colored Plastic Mulch Films Affecting Spider Mites And Aphids On Winter Strawberry In A High Tunnel, Kevin Philip Durden

Graduate Theses and Dissertations

Off season high tunnel strawberry production has the potential to augment income for Arkansas fruit growers during an unproductive time of the year, however management guidelines do not exist. At the University of Arkansas Agricultural Research and Extension Center in Fayetteville, AR studies were conducted to evaluate the effects of four nitrogen (46-0-0 urea fertilizer) fertigation rates (0.75, 0.50, 0.25, 0.0 kg N/day/ha) and red or black colored plastic mulch films on densities of twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), numbers of parasitized cotton aphids, and fruit yield weight. Foliar and …

Push-Pull: A Novel Ipm Strategy For The Green Revolution In Africa, Zeyaur R. Khan, Charles A.O. Midega, Jimmy O. Pittchar, John A. Pickett

All Peer-Reviewed Publications

Africa faces serious challenges in feeding its population, mainly due to poor yields of cereals that serve as both staple and cash crops occasioned by insect pests, weeds, and poor soil fertility, and more recently effects of climate change. A novel IPM approach dubbed push-pull has been developed and implemented in eastern Africa, based on locally available companion plants, that effectively addresses these constraints resulting in substantial grain yield increases. The technology involves intercropping cereal crops with stemborer moth repellent crops (push), the forage legume desmodium or molasses grass, and planting the attractive Napier grass (pull) as a border crop. …

Evaluating Green Stink Bug Damage And Insect Abundance In Edamame, Benjamin Carroll Thrash

Graduate Theses and Dissertations

Edamame are an emerging crop in Arkansas, with 2013 planting estimates at 680 hectares. Although edamame are the same species as conventional soybeans grown in Arkansas, differences in use, maturity, and harvest may require creation and implementation of insect pest management approaches that differ from those used in conventional soybean production systems in order for a quality crop to be produced. Studies were conducted to determine a green stink bug damage-density relationship for edamame. Cages were used to confine green stink bugs on field-planted edamame at densities of 0, 2, 6, or 12 green stink bugs per cage (0, 0.55, …

Similar Differentiation Patterns Between Pbp Expression Levels And Pheromone Component Ratios In Two Populations Of Sesamia Nonagrioides, Nicolas Glaser, Brigitte Frérot, Ene Leppik, Christelle Monsempes, Claire Capdevielle-Dulac, Bruno Le Ru, Thomas Lecocq, Myriam Harry, Emmanuelle Jacquin-Joly, Paul Andre Calatayud

All Peer-Reviewed Publications

Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured …

Repair Of The Proboscis Of Brush-Footed Butterflies (Lepidoptera: Nymphalidae), Suellen Pometto

All Theses

A key feature of the order Lepidoptera is the coilable proboscis, present in over 99% of lepidopteran species. The proboscis is used to obtain liquid nutrition, usually floral nectar. The proboscis is assembled from two elongate galeae immediately after emergence of the adult from the pupa. What happens if the galeae become separated? I studied the process of repair of the proboscis, behaviorally and functionally, at the organismal level. My research questions were as follows: 1) is the proboscis capable of repair, 2) is saliva necessary to proboscis repair, and 3) is the repaired proboscis able to acquire fluids? Test …

Collective Behaviour Without Collective Order In Wild Swarms Of Midges, Alessandro Attanasi, Andrea Cavagna, Lorenzo Del Castello, Irene Giardina, Stefania Melillo, Leonardo Parisi, Oliver Pohl, Bruno Rossaro, Edward Shen, Edmondo Silvestri, Massimilano Viale

Publications and Research

Collective behaviour is a widespread phenomenon in biology, cutting through a huge span of scales, from cell colonies up to bird flocks and fish schools. The most prominent trait of collective behaviour is the emergence of global order: individuals synchronize their states, giving the stunning impression that the group behaves as one. In many biological systems, though, it is unclear whether global order is present. A paradigmatic case is that of insect swarms, whose erratic movements seem to suggest that group formation is a mere epiphenomenon of the independent interaction of each individual with an external landmark. In these cases, …

Population And Demographic Structure Of Ixodes Scapularis Say In The Eastern United States., Joyce M. Sakamoto, Jerome Goddard, Jason L. Rasgon

CALS Publications

INTRODUCTION: The most significant vector of tick-borne pathogens in the United States is Ixodes scapularis Say (the blacklegged tick). Previous studies have identified significant genetic, behavioral and morphological differences between northern vs. southern populations of this tick. Because tick-borne pathogens are dependent on their vectors for transmission, a baseline understanding of the vector population structure is crucial to determining the risks and epidemiology of pathogen transmission. METHODS: We investigated population genetic variation of I. scapularis populations in the eastern United States using a multilocus approach. We sequenced and analyzed the mitochondrial COI and 16S genes and three nuclear genes (serpin2, …

Development And Optimization Of The Suna Trap As A Tool For Mosquito Monitoring And Control, Alexandra Hiscox, Bruno Otieno, Anthony Kibet, Collins K. Mweresa, Philemon Omusula, Martin Geier, Andreas Rose, Wolfgang R. Mukabana, Willem Takken

All Peer-Reviewed Publications

Background: Monitoring of malaria vector populations provides information about disease transmission risk, as well as measures of the effectiveness of vector control. The Suna trap is introduced and evaluated with regard to its potential as a new, standardized, odour-baited tool for mosquito monitoring and control. Methods. Dual-choice experiments with female Anopheles gambiae sensu lato in a laboratory room and semi-field enclosure, were used to compare catch rates of odour-baited Suna traps and MM-X traps. The relative performance of the Suna trap, CDC light trap and MM-X trap as monitoring tools was assessed inside a human-occupied experimental hut in a semi-field …

Effects Of Plant Virus And Its Insect Vector On Encarsia Formosa, A Biocontrol Agent Of Whiteflies, Xiaoyuan Liu, Wensheng Xiang, Xiaoguo Jiao, Youjun Zhang, Wen Xie, Qingjun Wu, Xuguo Zhou, Shaoli Wang

Entomology Faculty Publications

In this study, we investigated the tritrophic interactions among a persistently transmitted plant virus, Tomato yellow leaf curl virus (TYLCV), its insect vector, the sweetpotato whitefly Bemisia tabaci, and a parasitoid, Encarsia formosa Gahan, one of the most extensively used biological control agents. As an emerging invasive pest worldwide, the two most damaging whiteflies are B. tabaci B and Q cryptic species. On healthy tomato plants, parasitoid-induced mortality was significantly higher in B. tabaci B than in Q. In contrast, similar mortality levels of B and Q were observed on TYLCV-infected plants. A higher rate of parasitism was consistently observed …

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Nebline, July 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Table Setting Contest is a Fun Way to Learn Nutrition, Setting a Table, Presenting and More!

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Transcriptome Of The Female Synganglion Of The Black-Legged Tick Ixodes Scapularis (Acari: Ixodidae) With Comparison Between Illumina And 454 Systems, Noble Egekwu, Daniel E. Sonenshine, Brooke W. Bissinger, R. Michael Roe

Biological Sciences Faculty Publications

Illumina and 454 pyrosequencing were used to characterize genes from the synganglion of female Ixodes scapularis. GO term searching success for biological processes was similar for samples sequenced by both methods. However, for molecular processes, it was more successful for the Illumina samples than for 454 samples. Functional assignments of transcripts predicting neuropeptides, neuropeptide receptors, neurotransmitter receptors and other genes of interest was done, supported by strong e-values (<-6), and high consensus sequence alignments. Transcripts predicting 15 putative neuropeptide prepropeptides ((allatostatin, allatotropin, bursicon α, corticotropin releasing factor (CRF), CRF-binding protein, eclosion hormone, FMRFamide, glycoprotein A, insulin-like peptide, ion transport peptide, myoinhibitory peptide, inotocin ( = neurophysin-oxytocin), Neuropeptide F, sulfakinin and SIFamide)) and transcripts predicting receptors for 14 neuropeptides (allatostatin, calcitonin, cardioacceleratory peptide, corazonin, CRF, eclosion hormone, gonadotropin-releasing hormone/AKH-like, insulin-like peptide, neuropeptide F, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin) are reported. Similar to Dermacentor variabilis, we found transcripts matching pro-protein convertase, essential for converting neuropeptide hormones to their mature form. Additionally, transcripts predicting 6 neurotransmitter/neuromodulator receptors (acetylcholine, GABA, dopamine, glutamate, octopamine and …

Use Of Macroinvertebrates In Bioassessment Of Land Use And Water Quality In Northern Mongolia, Oyunchuluun Yadamsuren

All Dissertations

Land-use practices in Mongolia lead to habitat degradation and consequently affect the structure and function of biological communities. There is no accepted bioassessment technique for determining the ecological consequences of habitat degradation on biological communities and water quality in Mongolia, such that a monitoring and management program suitable for Mongolia is sorely needed. Both a trait-based approach and the North American Rapid Bioassessment Protocol (RBP) metrics were tested with macroinvertebrates to determine the performance and applicability of these approaches for predicting general ecological responses of freshwater and terrestrial communities to habitat variation due to overgrazing and mining in northern Mongolian …

Pyriproxyfen For Mosquito Control: Female Sterilization Or Horizontal Transfer To Oviposition Substrates By Anopheles Gambiae Sensu Stricto And Culex Quinquefasciatus, Oscar Mbare, Steven W. Lindsay, Ulrike Fillinger

All Peer-Reviewed Publications

Background: The use of gravid mosquitoes as vehicles to auto-disseminate larvicides was recently demonstrated for the transfer of pyriproxyfen (PPF) by container-breeding Aedes mosquitoes and presents an appealing idea to explore for other disease vectors. The success of this approach depends on the female's behaviour, the time of exposure and the amount of PPF that can be carried by an individual. We explore the effect of PPF exposure at seven time points around blood feeding on individual Anopheles gambiae sensu stricto and Culex quinquefasciatus fecundity and ability to transfer in laboratory assays. Method. Mosquitoes were exposed to 2.6 mg PPF …

Evaluation Of The Influence Of Electric Nets On The Behaviour Of Oviposition Site Seeking Anopheles Gambiae S.S, Sisay Dugassa, Jenny M. Lindh, Steve J. Torr, Steven W. Lindsay, Ulrike Fillinger

All Peer-Reviewed Publications

Background: Electric nets (e-nets) are used to analyse the flight behaviour of insects and have been used extensively to study the host-oriented flight of tsetse flies. Recently we adapted this tool to analyse the oviposition behaviour of gravid malaria vectors, Anopheles gambiae s.s., orienting towards aquatic habitats and traps by surrounding an artificial pond with e-nets and collecting electrocuted mosquitoes on sticky boards on the ground next to the nets. Here we study whether e-nets themselves affect the responses of gravid An. gambiae s.s. Methods. Dual-choice experiments were carried out in 80 m2 screened semi-field systems where 200 gravid An. …

Cercyonis Pegala Agawamensis (Satyridae): A New Butterfly Subspecies From The Coastal Salt Marshes Of The Northeastern United States Of America, Matthew D. Arey, Alex Grkovich

The Taxonomic Report of the International Lepidoptera Survey

A new subspecies of the Common Wood Nymph (Cercyonis pegala) from the New England coastline in the northeastern region of the United States is described on the basis of phenotype, behavior, habitat, seasonality, flight period, and phenology. Agawamensis is univoltine as in all pegala. The newly described subspecies occurs almost entirely in coastal salt marshes and estuarine meadows, quite atypical as understood for Cercyonis pegala. The newly described subspecies agawamensis predominately prefers and occurs within large open salt marshes but can also be found in similar habitat along smaller tidal rivers, coastal inlets and streams

A Case Of Sympatric Celastrina Ladon (Cramer), Celastrina Lucia (W. Kirby) And Celastrina Neglecta (Edwards) (Lycaenidae: Polyommatinae) In Northern Virginia, With Additional Records Of C. Lucia In Virginia, Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

A case of fully sympatric Celastrina ladon, C. lucia, and early spring brood C. neglecta is documented at a site in northern Virginia. Observations indicate that all three species occupy the same habitat, fly during the same flight period and utilize the same hostplant with no evidence of hybridization. C. ladon and C. lucia are obligate univoltines while C. neglecta is multivoltine. A later flight (second brood) of Celastrina neglecta at the same site utilizes eriophyid mite-induced leaf galls on the very same host tree species. Additional Virginia records of C. lucia are documented.

Larval Host Plants Of Enodia Anthedon, Satyrodes Appalachia And S. Eurydice In Vermont, Usa, David J. Hoag

The Taxonomic Report of the International Lepidoptera Survey

Field observation and captive rearing was used to clarify larval host plant use in Enodia anthedon, Satyrodes appalachia, and S. eurydice in Vermont, USA. In nature S. appalachia larvae were found on Carex lacustris, C. lupulina, C. gracillima, and C. tuckermanii. Enodia anthedon in nature was found to oviposit on grass and on Carex lacustris. Larvae of E. anthedon were found on C. lacustris and C. lupulina. In captivity, all but two E. anthedon larvae chose Carex over grass. Both E. appalachia and E. anthedon …

Wonders Of Wisconsin: A Study On Insect Macrophotography, Brenna L. Decker

Lawrence University Honors Projects

This past year I have been honing my skills as an entomologist and as a photographer. My solo exhibition “Wonders of Wisconsin: A Study on Insect Macrophotography” not only presents my personal progress, but also represents an overarching theme of a liberal arts education: connectivity. Everything we see or learn on campus and throughout life is connected. This audience-engaging exhibition has provided a visual for the connections between the fields of science and studio art, the art movements of New Objectivity and Relational Aesthetics, and between human and insect life.

The final exhibition opening on May 1^st at 5:30pm …