Entomology Commons^™

Lymesim 2.0: An Updated Simulation Of Blacklegged Tick (Acari: Ixodidae) Population Dynamics And Enzootic Transmission Of Borrelia Burgdorferi (Spirochaetales: Spirochaetaceae), Holly Gaff, Rebecca J. Eisen, Lars Eisen, Robyn Nadolny, Jenna Bjork, Andrew J. Monaghan

Biological Sciences Faculty Publications

Lyme disease is the most commonly reported vector-borne disease in the United States, and the number of cases reported each year continues to rise. The complex nature of the relationships between the pathogen (Borrelia burgdorferi sensu stricto), the tick vector (Ixodes scapularis Say), multiple vertebrate hosts, and numerous environmental factors creates challenges for understanding and predicting tick population and pathogen transmission dynamics. LYMESIM is a mechanistic model developed in the late 1990s to simulate the life-history of I. scapularis and transmission dynamics of B. burgdorferi s.s. Here we present LYMESIM 2.0, a modernized version of LYMESIM, that includes …

The Biofiltration Ability Of Asparagus Densiflorus To Remove Sulfur Dioxide From The Indoor Atmosphere, Rhiannon De La Rosa, Mary Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Sulfur dioxide is an inorganic compound (IC) and air pollutant that causes health risks in humans. The buildup of sulfur dioxide (SO2) in enclosed indoor spaces is, therefore, a concern to human health, especially since the average person spends 90% of his/her time indoors. This study focused on decreasing SO2 concentration in a cost-effective and simple way—by using botanical biofiltration, or the uptake of pollutants by plants. Research in biofiltration has focused mostly on the remediation of volatile organic compounds (VOC). However, research has also shown that plant species that remediate VOC efficiently also have the potential for efficient IC …

Dynamic Balancing Of Foraging And Defensive Effort Contribute To The Optimality Of The Honey Bee Robbing Strategy, Grayson James Grume

Theses and Dissertations--Entomology

The optimality of a foraging strategy shifts in response to dynamic ecological conditions and the need to devote effort to other tasks. Nest defense and foraging effort in the honey bee may trade off as both tasks are performed by a shared workforce of physiologically-specialized individuals in exclusive roles. Honey robbing is a foraging strategy predicted to benefit from simultaneous increases in foraging and defensive effort, but may be constrained by workforce specialization. We developed a methodology to induce robbing behaviors with uninhabited bait hives. We used this methodology to evaluate foraging and defensive effort before and during robbing by …

Predicting Patterns Of Gene Family Evolution In Taxa With Similar Ecological Niches, Kim Vertacnik

Theses and Dissertations--Biology

To fully understand the genetic basis of adaptation, we need to know its predictability—the extent to which specific selective pressures and contexts can yield corresponding genetic changes. In particular, the repeated colonization of similar, specialized environments by different taxa is ideal for assessing the frequency of reoccurring changes in the same genes or functions. But compared to a growing body of literature on the convergent evolution of individual genes, far less is known about the repeatability of gene family evolution, where families (defined here as groups of genes that share sequence and functional similarity from common ancestry) can expand (gain …

Aggregation Behavior In The Bed Bug, Cimex Lectularius L., Paul D. Baker

Theses and Dissertations--Entomology

The bed bug, Cimex lectularius L., is a common household pest that feeds on the blood of its human hosts. Unlike many other hematophagic arthropods, bed bugs have not demonstrated the ability to vector disease; yet its presence in a household often leads to fear and anxiety amongst its human host victims. Bed bugs spend the majority of their time aggregating in enclosed and hidden locations, making it difficult to detect and eradicate them. One of the significant mediators of aggregation behavior is an aggregation pheromone. The pheromone has been identified to be six chemical compounds, five volatile compounds that …

Management Of Sugarcane Aphid (Melanaphis Sacchari) Using Biological And Cultural Tactics In Kentucky, Nathan Harriman Mercer

Theses and Dissertations--Entomology

In 2013, a new aphid pest of sorghum, the sugarcane aphid Melanaphis sacchari Zehntner (Hemiptera: Aphididae), was found in the southern US, and caused significant yield loss in sorghum. Since then, M. sacchari has spread to most of the sorghum growing regions. Unmanaged populations can cause complete crop loss in grain, forage and sweet sorghum. M. sacchari cannot survive winters without a live plant tissue and must recolonize much of its pest range annually. Sweet sorghum (Sorghum bicolor) is an important crop for many growers in Kentucky, producing $16-25 million a year from its syrup. Biological control of …

Physiological Ecology Of Overwintering And Cold-Adapted Arthropods, Leslie Jean Potts

Theses and Dissertations--Entomology

Given their abundance and diversity, arthropods are an excellent system to investigate biological responses to winter. Winter conditions are being majorly impacted by climate change, and therefore understanding the overwintering biology of arthropods is critical for predicting ecological responses to climate change. In Chapters 2 and 3, I investigate the winter biology of a winter-active wolf spider. I show that winter-active spiders can take advantage of periodic prey resources and grown in the winter, which may allow them to get a jumpstart on spring reproduction. I also investigate spiders’ ability to track changes in their environment by quantifying low temperature …

Emerald Ash Borer Development Across A Latitudinal Gradient: Implications For Biocontrol, Sarah Pellecchia

Theses and Dissertations--Entomology

Emerald ash borer, EAB, (Agrilus planipennis Fairmaire) is an invasive beetle that kills ash trees. It was accidentally introduced from China, and has rapidly expanded across North America, now occupying much of the eastern US. Four classical biocontrol parasitoids have been released to help mitigate its spread and impact: Tetrastichus planipennisi Yang, Spathius agrili Yang, S. galinae Belokobylskij and Strazanac, and Oobius agrili Zhang and Huang. These parasitoids have been deployed throughout EAB’s invaded range, but there has been limited recovery of the parasitoids from their release sites in southern states. I evaluated whether this lack of establishment might …

Identification And Characterization Of Epigenetic Regulators In The Yellow Fever Mosquito, Aedes Aegypti, Shankar C. R. R. Chereddy

Theses and Dissertations--Entomology

Due to the increased interactions between the sylvatic and urban cycles of Aedes aegypti life cycle, there has been an increase in the incidence of vector-borne diseases. The risk involved through the transmission of viruses like Dengue, Zika, Yellow-fever and Chikungunya calls for an effective control method for the deadly vector. The possibility of pesticide resistance and the costs involved in producing new pesticides have pushed Sterile Insect Technique (SIT) into prominence. However, current methods used to sterize insects for SIT are not highly efficient. This study tackles current issues in mosquito control by identifying and characterizing epigenetic regulators that …

A Tangled Web: The Dynamics Of Endosymbiotic Infections In A Linyphiid Spider, Laura Cecilia Rosenwald

Theses and Dissertations--Entomology

Many arthropods are infected with bacterial endosymbionts that manipulate host reproduction, but few bacterial taxa have been shown to cause such manipulations. Mermessus fradeorum (Linyphiidae) is a sheet-weaving spider that displays both feminization and cytoplasmic incompatibility (CI). To correlate reproductive manipulations to endosymbionts, I surveyed the bacterial community of M. fradeorum using high throughput sequencing and found that individuals can be infected with up to five different strains of bacteria from the genera Wolbachia, Rickettsia, and Rickettsiella. Rickettsiella was found in all 23 tested spider matrilines. I used antibiotic curing to generate uninfected matrilines that I reciprocally crossed with …

Developing Alternative Practices For Management Of Flea Beetles Attacking Eggplant And Leafy Brassicaceous Greens, Robert Brockman

Theses and Dissertations--Entomology

Flea beetles are a challenging pest for many producers of vegetable crops in Kentucky. Chewing numerous small holes in the leaves of their host plants, high flea beetle populations can quickly overwhelm unestablished crops. I tested the efficacy of several alternatives to insecticides within brassicaceous leafy greens and eggplant. Four field trials in 2019 and 2020 compared essential oil sprays, the woven-mesh row cover ProtekNet, the spunbonded row cover Agribon, and reflective silver mulch to an untreated control, a conventional insecticide rotation of dinotefuran and pyrethroids, and an organic insecticide rotation of spinosad and pyrethrins. The silver reflective mulch was …

Management Of Stink Bugs (Hemiptera: Pentatomidae) On Soybean In Kentucky, Yaziri Gonzalez

Theses and Dissertations--Entomology

Phytophagous stink bugs (Hemiptera: Pentatomidae) are pests of significant importance to soybean, Glycine max (L.), because they can cause severe losses in seed quality, ultimately affecting yield. Damage to soybean is caused through a process called extra-oral digestion. This occurs when the adults and nymphs of stink bugs pierce plant tissues with their mandibular and maxillary stylets, insert digestive enzymes, break down tissue, and extract the digested plant fluid. Three endemic stink bug species of North America are present in Kentucky: green stink bug (Chinavia hilaris), brown stink bugs (Euschistus species complex), and red-shouldered stink bug (Thyanta custator) …

Epigenetic Regulation Of Hormone Action In The Red Flour Beetle, Tribolium Castaneum (Herbst), Smitha George

Theses and Dissertations--Entomology

Hormones are the chemical communication signaling molecules released into the body fluid to stimulate target cells of multicellular organisms. Two major hormones, ecdysteroids (20-hydroxyecdysone, 20E) and juvenile hormones (JH), regulate a wide variety of physiological and developmental processes in insects. Therefore, hormones have been extensively studied and are attractive targets for the development of target-specific insect control methods. Recent studies suggest that epigenetics adds another layer of regulation to explain multiple functions of the same circulating hormone in different tissues and at various time points. In my dissertation, I focused on a major post-translational modification, ‘acetylation,’ to elucidate the epigenetic …

Genetic Variation Within A Broadly Distributed Chewing Louse Genus (Thomomydoecus), Clarissa Elizabeth Bruns

Honors Program Theses

No broad study has been conducted to examine the genetics of Thomomydoecus species and their patterns of geographic variation. Chewing lice and their parasite-host relationships with pocket gophers have been studied as a key example of cophylogeny (Demastes et al., 2012). Despite this, genetic data on interspecific and intraspecific variation in Thomomydoecus is unexplored, and prior studies consisted within the narrow frame of one complex or species and its relative host gopher. This project collected, and analyzed genetic data, then generated phylogenetic trees. Many of the existing relationships between Thomomydoecus species was confirmed, and there were a number of unexpected …

Gaba Signaling Affects Motor Function In The Honey Bee, Julie A. Mustard, Lisa Jones, Geraldine A. Wright

School of Integrative Biological & Chemical Sciences Faculty Publications

GABA is the most common inhibitory neurotransmitter in both vertebrate and invertebrate nervous systems. In insects, inhibition plays important roles at the neuromuscular junction, in the regulation of central pattern generators, and in the modulation of information in higher brain processing centers. Additionally, increasing our understanding of the functions of GABA is important since GABAA receptors are the targets of several classes of pesticides. To investigate the role of GABA in motor function, honey bee foragers were injected with GABA or with agonists or antagonists specific for either GABAA or GABAB receptors. Compounds that activated either type of GABA receptor …

The First Mitochondrial Genome Of The Living-Fossil Sawfly Macroxyela Ferruginea (Hymenoptera: Xyelidae, Macroxyelinae), Bo-Ying Zheng, Ze-Kai Li, Xiao-Fei Li, Jia-Chen Zhu, Michael J. Sharkey, Pu Tang, Xue-Xin Chen

Entomology Faculty Publications

The living-fossil sawfly Macroxyela ferruginea (Xyelidae: Macroxyelinae) was one of the oldest species of Hymenoptera. We sequenced the mitochondrial genome, 15,465 bp in size. All 37 typical mitochondrial genes were possessed. There is only one rearrangement of gene order, where trnM and trnQ were shuffled. We also found this order was shared with Xyela sp., which also belongs to family Xyelidae. The 13 protein-coding genes of this sequence and the other 10 species from eight superfamilies in Hymenoptera were all used for phylogenetic analysis by maximum likelihood (ML) analysis and Bayesian inference (BI), with Ascaloptynx appendiculatus from Neuroptera as an …

Evaluation Of The Relationship Between Land Use And Water Quality In Kittitas County, Wa, Lindsay Schulz

All Master's Theses

Water in Kittitas County is extremely valuable since it supports farming, recreation, and cultural activities, as well as environmental processes and a diversity of biological life while providing many ecosystem services. However, land conversions required by agricultural and urban land uses can negatively impact water quality and the biological function of the stream. I studied how forested, agricultural, and urban land use affect six streams. Fourteen sites were sampled, once each in July, August, and September 2019. Land use was calculated as a percentage of forested, agricultural, and urban land use within a 100-m buffer of the stream, upstream of …

A Tale Of Winglets: Evolution Of Flight Morphology In Stick Insects, Yu Zeng, Connor O'Malley, Sonal Singhal, Faszly Rahim, Sehoon Park, Xin Chen, Robert Dudley

Publications and Research

The evolutionary transition between winglessness and a full-winged morphology requires selective advantage for intermediate forms. Conversely, repeated secondary wing reductions among the pterygotes indicates relaxation of such selection. However, evolutionary trajectories of such transitions are not well-characterized. The stick insects (Phasmatodea) exhibit diverse wing sizes at both interspecific and intersexual levels, and thus provide a system for examining how selection on flight capability, along with other selective forces, drives the evolution of flight-related morphology. Here, we examine variation in relevant morphology for stick insects using data from 1,100+ individuals representing 765 species. Although wing size varies along a continuous spectrum, …

Trees, Fungi, Insects: How Host Plant Genetics Builds A Community, Sandra Jeanne Simon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organisms, such as fungi and insects, can cause millions of acres of agricultural and forest damage, while others provide billions of dollars in ecological services such as education, aesthetic enjoyment, pollination, and gardening. Plant breeding and biotechnology can potentially help establish a balance between the proliferation of detrimental pests and attraction of beneficial insects. Variation in plant physiological and morphological characteristics are extremely important in the ability of host tissues to support many different types of organisms. When that variation is genetically heritable in a plant population, shifts in the underlying genes can have predictable consequences in structuring entire ecosystems. …

Field Efficacy Of Soil Insecticides On Pyrethroid-Resistant Western Corn Rootworms (Diabrotica Virgifera Virgifera Leconte), Dariane Souza, Julie A. Peterson, Robert Wright, Lance Meinke

Department of Entomology: Faculty Publications

Background: Field-evolved pyrethroid resistance has been confirmed in western corn rootworm (WCR) populations collected from the United States (US) western Corn Belt. Resistance levels of WCR adults estimated in lab bioassays were confirmed to significantly reduce the efficacy of foliar-applied bifenthrin. The objective of the present study was to investigate the impact of WCR pyrethroid resistance levels on the performance of common soil-applied insecticide formulations (23.4% tefluthrin, 17.15% bifenthrin, and 0.1% cyfluthrin+2.0% tebupirimphos). Field trials were conducted in 2016 and 2017 in three Nebraska, US, counties (Saunders, Clay, and Keith) where distinct levels of WCR susceptibility topyrethroids (susceptible, moderately resistant, …

Description Of The Immature Stages Of Stenocrates Agricola Dechambre & Hardy (Coleoptera: Scarabaeidae: Dynastinae: Cyclocephalini) With Redescription Of The Adult And Discussion Of Its Tribal Position, Mario G, Ibarra-Polesel, Jhon C. Neita-Moreno, Brett Ratcliffe

Department of Entomology: Faculty Publications

The enigmatic genus Stenocrates Burmeister has 52 species that are widely distributed from Mexico to Argentina, but mainly in South America. In the present study, the larva of the third instar and pupa of Stenocrates agricola Dechambre & Hardy, 2004 are described and illustrated based on specimens from Argentina, this being the first description of immatures for the genus. The adult is also redescribed, significantly expanding the number of characters and providing information on the natural history of this species. In addition, 28 species of Cyclocephalini and 20 species of Pentodontini are morphologically compared, emphasizing the most useful larval characters …

Methods For Surveying Stable Fly Populations, David B. Taylor, Kyle Harrison, Junwei J. Zhu

Department of Entomology: Faculty Publications

Stable flies are among the most important pests of livestock throughout much of the world. Their painful bites induce costly behavioral and physiological stress responses and reduce productivity. Stable flies are anthropogenic and their population dynamics vary depending on agricultural and animal husbandry practices. Standardized sampling methods are needed to better identify the factors controlling stable fly populations, test novel control technologies, and determine optimal management strategies. The current study reviewed methods used for a long-term study of stable fly population dynamics in the central Great Plains. An additional study compared the relative size of flies sampled from the general …

Terpenoid-Induced Feeding Deterrence And Antennal Response Of Honey Bees, Nicholas R. Larson, Scott T. O'Neal, Ulrich R. Bernier, Jeffrey R. Bloomquist, Troy D. Anderson

Department of Entomology: Faculty Publications

Multiple interacting stressors negatively affect the survival and productivity of managed honey bee colonies. Pesticides remain a primary concern for beekeepers, as even sublethal exposures can reduce bee immunocompetence, impair navigation, and reduce social communication. Pollinator protection focuses on pesticide application guidelines; however, a more active protection strategy is needed. One possible approach is the use of feeding deterrents that can be delivered as an additive during pesticide application. The goal of this study was to validate a laboratory assay designed to rapidly screen compounds for behavioral changes related to feeding or feeding deterrence. The results of this investigation demonstrated …

Ento(O)Mics: The Intersection Of ‘Omic’ Approaches To Decipher Plant Defense Against Sap-Sucking Insect Pests, Prince Zogli, Lise Pingault, Sajjan Grover, Joe Louis

Department of Entomology: Faculty Publications

Plants are constantly challenged by insect pests that can dramatically decrease yields. Insects with piercing-sucking mouthparts, for example, aphids, whiteflies, and leaf hoppers, seemingly cause less physical damage to tissues, however, they feed on the plant’s sap by piercing plant tissue and extracting plant fluids, thereby transmitting several plantpathogenic viruses as well. As a counter-defense, plants activate an array of dynamic defense machineries against insect pests including the rapid reprogramming of the host cell processes. For a holistic understanding of plant-sap-sucking insect interactions, there is a need to call for techniques with the capacity to concomitantly capture these dynamic changes. …

First Description Of The Female Of The Rare Megasoma Lecontei Hardy (Coleoptera: Scarabaeidae: Dynastinae: Dynastini), Brett Ratcliffe, Guillermo Nogueira, Rich Cunningham

Department of Entomology: Faculty Publications

Megasoma lecontei Hardy is the most rarely collected of all species of Megasoma Kirby, and the female remained undescribed. A description and illustrations of the female of M. lecontei are provided for the first time based on several specimens from near Cabo San Lucas, Baja California Sur, Mexico. A revised key to the Megasoma species occurring in Baja California Sur, Mexico is presented.

Phenology And Dispersal Of The Wheat Stem Sawfly (Hymenoptera: Cephidae) Into Winter Wheat Fields In Nebraska, Chris T. Mccullough, Gary L. Hein, Jeff Bradshaw Dr.

Department of Entomology: Faculty Publications

Historically, the wheat stem sawfly, Cephus cinctus Norton was a pest in spring wheat-growing regions of the northern Great Plains. However, in the 1980s, it was found infesting winter wheat fields in Montana. Infestations were first detected in western Nebraska in the 1990s, and have since spread throughout the Nebraska Panhandle. Larval damage occurs from stem-mining, but stem girdling that results in lodged stems that are not harvested results in the greatest yield losses.The biology and phenology of the wheat stem sawfly are well described in the northern portion of its range, but they are lacking in Colorado, southeast Wyoming, …

Description Of A New Species Of Gymnetis Macleay (Coleoptera: Scarabaeidae: Cetoniinae: Gymnetini) From Colima, Mexico, Brett Ratcliffe, Guillermo Nogueira

Department of Entomology: Faculty Publications

Gymnetis pedroreyesi Ratcliffe and Nogueira, new species, is described from the state of Colima, Mexico. The description is accompanied by illustrations, a diagnosis to distinguish it from similar species, observations on natural history, and a key to the 12 Mexican species of Gymnetis MacLeay, 1819.

Gymnetis pedroreyesi Ratcliffe y Nogueira, nueva especie, es descrita del Estado de Colima,M´exico. La descripci´on es acompa~nada por ilustraciones, diagnosis para distinguirla de especies similares, observaciones y caracter´ısticas de su h´abitat y una clave para las 12 especies mejicanas de Gymnetis MacLeay, 1819.

Genome Sequence Of A Small Rna Virus Of The Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi Barber (Coleoptera: Chrysomelidae), Sijun Liu, Arnubio Valencia-Jiménez, Molly Darlington, Ana M. Vélez, Bryony C. Bonning

Department of Entomology: Faculty Publications

The genome sequence of a novel small RNA virus, tentatively named Diabrotica undecimpunctata virus 1 (DuV1), was discovered from the transcriptome of the southern corn rootworm, Diabrotica undecimpunctata howardi Barber. DuV1 has a positive-sense, single-stranded RNA genome that encodes a single polyprotein of 3,401 amino acids with limited similarity to other viruses.

Maize Defense Elicitor, 12-Oxo-Phytodienoic Acid, Prolongs Aphid Salivation, Sajjan Grover, Suresh Varsani, Michael V. Kolomiets, Joe Louis

Department of Entomology: Faculty Publications

12-Oxo-phytodienoic acid (OPDA), an intermediate in the jasmonic acid (JA) biosynthesis pathway, regulates diverse signaling functions in plants, including enhanced resistance to insect pests. We previously demonstrated that OPDA promoted enhanced callose accumulation and heightened resistance to corn leaf aphid (CLA; Rhopalosiphum maidis), a phloem sap-sucking insect pest of maize (Zea mays). In this study, we used the electrical penetration graph (EPG) technique to monitor and quantify the different CLA feeding patterns on the maize JA-deficient 12-oxo- phytodienoic acid reductase (opr7opr8) plants. CLA feeding behavior was unaffected on B73, opr7opr8 control plants (- OPDA), and opr7opr8 plants that were pretreated …

Greenbug (Schizaphis Graminum) Herbivory Significantly Impacts Protein And Phosphorylation Abundance In Switchgrass (Panicum Virgatum), Prince Zogli, Sophie Alvarez, Michael J. Naldrett, Nathan A. Palmer, Kyle G. Koch, Lise Pingault, Jeffrey D. Bradshaw, Paul Twigg, Tiffany M. Heng‐Moss, Joe Louis, Gautam Sarath, Joe Louis

Department of Entomology: Faculty Publications

Switchgrass (Panicum virgatum L.) is an important crop for biofuel production but it also serves as host for greenbugs (Schizaphis graminum Rondani; GB). Although transcriptomic studies have been done to infer the molecular mechanisms of plant defense against GB, little is known about the effect of GB infestation on the switchgrass protein expression and phosphorylation regulation. The global response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored

by label‐free proteomics shotgun in GB‐infested and uninfested control plants at 10 days post infestation. Peptides matching a total of 3,594 proteins were identified and 429 were differentially expressed proteins in …