Entomology Commons^™

Honey Bee Foraged Pollen Reveals Temporal Changes In Pollen Protein Content And Changes In Forager Choice For Abundant Versus High Protein Flowers, Gabriela Quinlan, Meghan Milbrath, Clint Otto, Autumn Smart, Deborah D. Iwanowicz, Robert Cornman, Rufus Isaacs

Department of Entomology: Faculty Publications

Protein derived from pollen is an essential component of healthy bee diets. Protein content in honey bee foraged pollen varies temporally and spatially, but the drivers underlying this variation remain poorly characterized. We assessed the temporal and spatial variation in honey bee collected pollen in 12 Michigan apiaries over 3 summers (2015–2017). We simultaneously monitored forage in flowering habitats (uncultivated floristically-rich areas and conservation program land) near these apiaries throughout the growing season. We used these data, along with data from the literature on plant pollen protein content, to determine if honey bees collected a greater proportion of pollen from …

A New Subspecies Of Lon Taxiles (W. H. Edwards, 1861) (Lepidoptera: Hesperiidae) From The Great Plains, Stephen M. Spomer

Department of Entomology: Faculty Publications

A new subspecies of Lon taxiles (W. H. Edwards, 1861) is described from the Pine Ridge of Nebraska and Black Hills of South Dakota. It is distinguished by the presence of distinct white spots on the VHW of females and darker males than specimens from other parts of its range.

The Taxiles skipper, Lon [formerly Poanes; see Cong et al. (2019)] taxiles (W. H. Edwards, 1861), is a woodland species found in mountainous areas in extreme southeastern Idaho, through Colorado and Utah, south through New Mexico and Arizona, into Mexico. There is also a somewhat disjunct eastern population, ranging from …

Challenges For Adoption Of Integrated Pest Management (Ipm): The Soybean Example, A. F. Bueno, A. R. Panizzi, Thomas E. Hunt, P. M. Dourado, R. M. Pitta, J. Gonçalves

Department of Entomology: Faculty Publications

Soybean is considered one of today’s most important crops. Planted on millions of hectares worldwide, the management of soybean pests usually requires large amounts of chemicals. However, a key component to meet the increasing demand for food due to the rapidly growing global population is protecting crops from pests while maintaining environmental quality through ecologically and economically sound integrated pest management (IPM) practices. Not only can IPM result in more profitable agriculture due to the reduction of pest control costs but also assures equitable, secure, sufficient, and stable flows of both food and ecosystem services. Despite those ecological and economic …

A First Flight In North Carolina—But Not At Kitty Hawk, Robert J. Wright

Department of Entomology: Faculty Publications

The southern corn billbug is a univoltine insect most common in the Coastal Plains region of North Carolina, Virginia, Georgia, and South Carolina. However, this insect can also be found in the midwestern U.S. (Van Duyn and Wright 1999). Early in the growing season, the adults can be found feeding at the base of corn plants—typically with their head pointed downward (Fig. 1)—by inserting their beak into the base of the plant. They can kill or stunt seedlings or injure the developing leaves inside the plant, causing twisted leaves as they emerge or a series of small holes across the …

Frederick Baxendale (1949–2021), Robert J. Wright, Tiffany Heng-Moss

Department of Entomology: Faculty Publications

Frederick “Fred” Paul Baxendale, 72, of Lincoln, Nebraska, passed away Thursday, 30 September 2021. He was born on 10 April 1949 to Ralph and Inez (Thrasher) Baxendale in Rochester, NY. After graduating from Williamson Central High School, he served in the United States Air Force Air Weather Service from 1969 to 1973. He received an A.S. in Mathematics and Science from Finger Lakes Community College in 1975, and a B.S. in Entomology from Cornell University in 1977. He received both his M.S in Entomology (1980) and his Ph.D. from Texas A&M University in 1983, majoring in Entomology. Fred was a …

Transference Of Citizen Science Program Impacts: A Theory Grounded In Public Participation In Scientific Research, Louise I. Lynch-O’Brien, Wayne A. Babchuk, Jenny M. Dauer, Tiffany Heng-Moss, Doug Golick

Department of Entomology: Faculty Publications

Citizen science is known for increasing the geographic, spatial, and temporal scale from which scientists can gather data. It is championed for its potential to provide experiential learning opportunities to the public. Documentation of educational outcomes and benefits for citizen scientists continues to grow. This study proposes an added benefit of these collaborations: the transference of program impacts to individuals outside of the program. The experiences of fifteen citizen scientists in entomology citizen science programs were analyzed using a constructivist grounded theory methodology. We propose the substantive-level theory of transference to describe the social process by which the educational and …

Development And First Evaluation Of An Attractant Impregnated Adhesive Tape Against Blood-Sucking Flies, Junwei J. Zhu, Gwang-Hyun Roh, Yosuke Asamoto, Kujtim Bizati, Jen-Chieh Liu, Alexander Lehmann, Kyle Harrison, David B. Taylor, Hironao Otake

Department of Entomology: Faculty Publications

Stable flies are one of the most important arthropod pests of livestock that reduce cattle weight gain and milk production leading to annual economic losses in excess of $2 billion to the US cattle industry. The host-seeking behavior is primarily mediated by associated odors from stable fly larval development environments and host animals. The present paper reports the development and evaluation of attractant-impregnated adhesive tapes to reduce stable fly attacks on cattle. Laboratory bioassays showed that only m-cresol impregnated adhesive tapes caught significantly more stable flies (16 ± 1) than the control tape without attractant added (7 ± 1), with …

Patch Utilization And Flower Visitations By Wild Bees In A Honey Bee-Dominated, Grassland Landscape, Clint R. V. Otto, Larissa L. Bailey, Autumn H. Smart

Department of Entomology: Faculty Publications

Understanding habitat needs and patch utilization of wild and managed bees has been identified as a national research priority in the United States. We used occupancy models to investigate patterns of bee use across 1030 transects spanning a gradient of floral resource abundance and richness and distance from apiaries in the Prairie Pothole Region (PPR) of the United States. Estimates of transect use by honey bees were nearly 1.0 during our 3.5-month sampling period, suggesting honey bees were nearly ubiquitous across transects. Wild bees more frequently used transects with higher flower richness and more abundant flowers; however, the effect size …

Hitchhiking Or Hang Gliding? Dispersal Strategies Of Two Cereal-Feeding Eriophyoid Mite Species, Agnieszka Majer, Alicja Laska, Gary Hein, Lechosław Kuczyński, Anna Skoracka

Department of Entomology: Faculty Publications

Dispersal shapes the dynamics of populations, their genetic structure and species distribution; therefore, knowledge of an organisms’ dispersal abilities is crucial, especially in economically important and invasive species. In this study, we investigated dispersal strategies of two phytophagous eriophyoid mite species: Aceria tosichella (wheat curl mite, WCM) and Abacarus hystrix (cereal rust mite, CRM). Both species are obligatory plant parasites that infest cereals and are of economic significance. We investigated their dispersal success using different dispersal agents: wind and vectors. We hypothesised that in both mite species the main mode of dispersal is moving via wind, whereas phoretic dispersal is …

The Dynastine Scarab Beetles (Coleoptera: Scarabaeidae: Dynastinae) Of Chile, Brett C. Ratcliffe, Ronald D. Cave, José Mondaca

Department of Entomology: Faculty Publications

The eight species of dynastine scarab beetles that occur in Chile are reviewed. Keys, descriptions, geographic distributions, monthly adult activity, notes on natural history, illustrations, and distribution maps are provided for all species. The erroneous records of 16 dynastine species reported from Chile are reviewed and clarified. The monotypic genus Chiliphileurus Endrödi, 1977 is reduced to junior synonymy with Eophileurus Arrow, 1908, and its only species, Chiliphileurus tuberculatus Endrödi, 1977, follows as a new junior synonym of the Asian Eophileurus cingalensis Arrow, 1908.

A New Species Of Hope, 1837 (Coleoptera: Scarabaeidae: Dynastinae: Oryctini) From Peru, Brett C. Ratcliffe, Jochen-P. Saltin

Department of Entomology: Faculty Publications

Megaceras sarecagem Ratcliffe and Saltin is described as a new species from Oxapampa in the Pasco Region of Peru. A description, diagnosis separating it from similar species, illustrations, and a distribution map are provided.

Solifuge (Camel Spider) Reproductive Biology: An Untapped Taxon For Exploring Sexual Selection, Alfredo V. Peretti, David E. Vrech, Eileen Hebets

Eileen Hebets Publications

The exploration of new and diverse animal groups in the study of sexual selection is both necessary and important to help better understand broad patterns and test sexual selection hypotheses regarding the evolutionary origins and maintenance of reproductive tactics and associated traits. Solifuges are, in this matter, an exceptional group and very little explored from the sexual selection point of view. At first glance, mating is apparently quite simple and conserved within this arachnid order, but solifuge reproductive behavior is unique among arachnids and more diverse than previously thought. In particular, these voracious animals appear to exhibit high sexual conflict, …

Spiders (Araneae) Collected As Prey By The Mud-Dauber Wasps Sceliphron Caementarium And Chalybion Californicum (Hymenoptera: Sphecidae) In Southeastern Nebraska1, Tyler B. Corey, Earl Agpawa, Eileen Hebets

Eileen Hebets Publications

Predator diets represent a potential interaction between local prey availability, prey antipredator defenses, and predator foraging behavior. Female spider-specialist muddauber wasps (Hymenoptera: Sphecidae) collect spiders and provision them intact, but paralyzed, to their developing larvae, providing a unique means of quantifying the diversity and abundance of prey that they capture. Mud-dauber wasps are hypothesized to be a major source of selection on antipredator defenses in web-building spiders, and the spiny and thickened abdomens of female spiny orb-weaving spiders (Araneae: Araneidae) are hypothesized to function as antiwasp defenses. We inventoried spider prey from nests of the mud-dauber wasps Sceliphron caementarium (Drury) …

Multisensory Integration Supports Configural Learning Of A Home Refuge In The Whip Spider Phrynus Marginemaculatus, Kaylyn A.S. Flanigan, Daniel D. Wiegmann, Eileen Hebets, Verner Peter Bingman

Eileen Hebets Publications

Predator diets represent a potential interaction between local prey availability, prey antipredator defenses, and predator foraging behavior. Female spider-specialist muddauber wasps (Hymenoptera: Sphecidae) collect spiders and provision them intact, but paralyzed, to their developing larvae, providing a unique means of quantifying the diversity and abundance of prey that they capture. Mud-dauber wasps are hypothesized to be a major source of selection on antipredator defenses in web-building spiders, and the spiny and thickened abdomens of female spiny orb-weaving spiders (Araneae: Araneidae) are hypothesized to function as antiwasp defenses. We inventoried spider prey from nests of the mud-dauber wasps Sceliphron caementarium (Drury) …

Visual Control Of Refuge Recognition In The Whip Spider Phrynus Marginemaculatus, Kaylyn A.S. Flanigan, Daniel D. Wiegmann, Patrick Casto, Vincent J. Coppola, Natasha R. Flesher, Eileen Hebets, Verner Peter Bingman

Eileen Hebets Publications

Amblypygids, or whip spiders, are nocturnally active arachnids which live in structurally complex environments. Whip spiders are excellent navigators that can re-locate a home refuge without relying on visual input. Therefore, an open question is whether visual input can control any aspect of whip spider spatial behavior. In the current study, Phrynus marginemaculatus were trained to locate an escape refuge by discriminating between differently oriented black and white stripes placed either on the walls of a testing arena (frontal discrimination) or on the ceiling of the same testing arena (overhead discrimination). Regardless of the placement of the visual stimuli, the …

Identification And Quantification Of 11 Airborne Biochemicals Emitted By The Brown Recluse And Another Primitive Hunting Spider Using Headspace Solid-Phase Microextraction-Gc/Ms, Zachary Foulks, Jennifer Parks, William Stoecker, Charles Kristensen, Eileen A. Hebets, Honglan Shi

Eileen Hebets Publications

Loxosceles reclusa, or brown recluse spider, is a harmful household spider whose habitat extends throughout the Midwest in the USA and other regions in the world. The pheromones and other biomolecules that facilitate signaling for brown recluses and other spider species are poorly understood. A rapid and sensitive method is needed to analyze airborne spider signaling biomolecules to better understand the structure and function of these biochemicals in order to control the population of the spiders. In this study, we developed a novel headspace solid-phase microextraction (HS-SPME)-GC/MS method to analyze potential pheromones and biomolecules emitted by the brown recluse …

There’S More Than One Way To Beat The Heat: What Anal Probes Reveal About Thermal Responses In Neotropical Beetles (Coleoptera: Cerambycidae)., Emilie I. Dion

Dissertations and Theses

Increasing global temperatures have been linked to shifts in the distributions and preferred microhabitats of terrestrial species. This trend is predicted to impact tropical forest insects, which may already be exposed to conditions that bring them close to their thermal maxima. Tropical microhabitats are linked to vertical stratification, because the canopy is hotter, winder, and less humid than the ground. In ectothermic organisms, such as beetles, thermoregulation is dependent on morphological and behavioral traits. This study investigates woodboring cerambycid beetles, to determine how behavioral thermoregulation through microhabitat selection (vertical stratification) is correlated to morphological attributes (color lightness, size, and pubescence) …

Weak Olfactory Preferences Of The Gall Midge Asphondylia Borrichiae, Associated Fungal Endophytes And Implications On Gene Flow And Host Range Expansion, Frances S. Nagle

UNF Graduate Theses and Dissertations

Asphondylia borrichaie is a small fly (Diptera: Cecidomyiidae) that is currently undergoing host-associated sympatric divergence. Asphondylia borrichaie is an ambrosia galler, these insects utilize a host plant for oviposition, but its offspring also rely on a symbiotic fungus (or fungal community) to promote the formation of the gall as well as serve as a food source for the developing larvae. Previous studies indicate that A. borrichaie consists of two host- associated populations based on its original host plant Borrichia frutescens (Asterales: Asteraceae), and another one from the two Iva species (I. frutescens (Asterales: Asteraceae) and I. imbricata …

The Influence Of Resting Metabolic Rate And Environment On Tonic Immobility In A Parasitoid Wasp (Hymenoptera: Pteromalidae), Ryan D. Morris

Graduate Research Theses & Dissertations

The effects of RMR (resting metabolic rate) and environment on tonic immobility were examined in the parasitoid wasp Spalangia cameroni (Order Hymenoptera: Family Pteromalidae). Tonic immobility (TI) is described as a state that an organism enters wherein they fall onto their back or side and become immobile for a period of time when disturbed. This state can last for several seconds to a few hours, depending on the organism in question. The organism may be aware during this period of time but unable to move. Female wasps were subjected to experimental treatments that involved manipulation of their environment, specifically, cold …

The Garden Oasis: Observations In A Southern California Garden, Chris Tankersley

Department of Entomology: Distance Master of Science Projects

A garden can be a special place of relaxation and a corridor from which one can connect with the natural world and observe the complex relationships among the many organisms that make it their home. These organisms include a vast array of insects that contribute to the diversity of even the smallest garden. We purchased an unassuming house in a quiet Southern California suburban neighborhood that included about half an acre of property made up of mostly lawn and a dense menagerie of backyard shrubs and trees on a steep hillside, along with a pond that was in desperate need …

Overview Of The Insect Food And Feed Industry, Daniel J. Seckman

Department of Entomology: Distance Master of Science Projects

The entomophagy field is one that when you immerse yourself in it, you will feel like the entire world is talking about insects as food and feed. Yet, from the outside, the industry is virtually nonexistent. In the United States, the North American Coalition for Insect Agriculture (NACIA) is the leading organization dedicated to growing the insect food and feed sector. While the organization has grown extensively in recent years, most individuals in the food, feed, and entomological industry have never heard of the organization. The Wall Street Journal presents the annual Food Forum, which aims to “tackle the critical …

Forensic Entomology: Insect Succession, Northern Colorado, Thomas Nissen

Department of Entomology: Distance Master of Science Projects

Forensic entomology is considered a relatively new field of entomology yet insects have been around for millions of years. Insects have been associated with dead animals and humans for centuries. The remains of insects have been recorded from Ancient Egypt to the present time. Recent advancements have been with data presented to the courts that show that there is a specific order to insects arriving on a corpse and that order helps to determine time of death. This study investigates the order of insect succession in the northern front range area of Colorado.

Microbial Contaminants In Wild Harvested And Traded Edible Long-Horned Grasshopper, Ruspolia Differens (Orthoptera: Tettigoniidae) In Uganda, S. Labu, S. Subramanian, F. M. Khamis, P. Akite, P. Kasangaki, M. Chemurot, C. M. Tanga, F. L.O. Ombura, J. P. Egonyu

All Peer-Reviewed Publications

This study investigated the relative abundance and identity of microbial contaminants of the edible long-horned grasshopper (Ruspolia differens) harvested from the wild and traded in informal markets in Uganda, to reveal high health risk points. Raw samples of whole R. differens were collected from wild vegetation, trapping sites and markets. Additionally, samples of plucked and deep-fried ready-to-eat R. differens were collected from the markets. The samples were cultured on standard media for microbial quantification, and pure cultures were characterised using molecular techniques. Bacterial and fungal counts in deep fried ready-to-eat R. differens were ~3 and 2-fold lower, respectively, than in …

Black Soldier Fly Larval Meal In Feed Enhances Growth Performance, Carcass Yield And Meat Quality Of Finishing Pigs, S. Y. Chia, C. M. Tanga, I. M. Osuga, A. O. Alaru, D. M. Mwangi, M. Githinji, T. Dubois, S. Ekesi, J. J.A. Van Loon, M. Dicke

All Peer-Reviewed Publications

Using black soldier fly (BSF, Hermetia illucens) larvae as a novel protein source substituting fishmeal (FM) in animal feeds is globally gaining momentum. BSF can be reared on agro-industrial residues. However, incorporating BSF larval meal (BSFLM) into finisher pig diets has received inadequate attention. This study evaluated the effects of replacing dietary FM with BSFLM on growth, carcass traits and meat quality of finisher pigs. A control diet (including FM: 0% BSFLM) was compared with four dietary levels of replacement of FM with BSFLM at 25, 50, 75 or 100%. Forty hybrid pigs (crossbreeds of purebred Large White and Landrace) …

Effect Of Zebra Skin-Derived Compounds On Field Catches Of The Human African Trypanosomiasis Vector Glossina Fuscipes Fuscipes, Olabimpe Y. Olaide, David P. Tchouassi, Abdullahi A. Yusuf, Christian W.W. Pirk, Daniel K. Masiga, Rajinder K. Saini, Baldwyn Torto

All Peer-Reviewed Publications

The riverine tsetse fly Glossina fuscipes fuscipes is a major vector of trypanosome pathogens causing African trypanosomiasis. This fly species uses a combination of olfactory and visual cues to locate its hosts. Previously, traps and targets baited with visual cues have been used in vector control, but the development of olfactory-based tools has been challenging. Recently, repellents have shown promise as olfactory-based tools in tsetse vector control. Here, we evaluated a three-component blend comprising 6-methyl-5-hepten-2-one, acetophenone and geranyl acetone (blend K), previously identified as a repellent for savannah tsetse flies in zebra skin odor, on G. f. fuscipes populations. Using …

Low-Cost Technology For Recycling Agro-Industrial Waste Into Nutrient-Rich Organic Fertilizer Using Black Soldier Fly, Dennis Beesigamukama, Benson Mochoge, Nicholas K. Korir, Komi K.M. Fiaboe, Dorothy Nakimbugwe, Fathiya M. Khamis, Sevgan Subramanian, Musyoka M. Wangu, Thomas Dubois, Sunday Ekesi, Chrysantus M. Tanga

All Peer-Reviewed Publications

Efforts to recycle organic waste using black soldier fly (BSF) larvae into high-quality alternative protein ingredients in animal feeds and organic fertilizers have gained momentum worldwide. However, there is limited information on waste manipulation to increase nutrient retention for enhanced larval performance and frass fertilizer quality. In the present study, brewer's spent grain with a carbon to nitrogen (C/N) ratio of 11 (control) was amended with sawdust to obtain substrates with C/N ratios of 15, 20, 25 and 30. The effects of substrate C/N ratios on BSF larval yield, waste degradation, biomass conversion efficiency, compost maturity and nutrient levels of …

Prevalence Of Asymptomatic P. Falciparum Gametocyte Carriage Among School Children In Mbita, Western Kenya And Assessment Of The Association Between Gametocyte Density, Multiplicity Of Infection And Mosquito Infection Prevalence., Victor A. Mobegi, Jeremy K. Herren, Abdoulie O. Touray, Fred Wamunyokoli, Hellen Butungi

All Peer-Reviewed Publications

Background: Asymptomatic Plasmodium falciparum gametocyte carriers are reservoirs for sustaining transmission in malaria endemic regions. Gametocyte presence in the host peripheral blood is a predictor of capacity to transmit malaria. However, it does not always directly translate to mosquito infectivity. Factors that affect mosquito infectivity include, gametocyte sex-ratio and density, multiplicity of infection (MOI), and host and vector anti-parasite immunity. We assess the prevalence of gametocyte carriage and some of its associated risk factors among asymptomatic schoolchildren in Western Kenya and to further analyse the association between gametocyte density, multiplicity of infection (MOI) and mosquito infection prevalence. Methods: P. falciparum …

Τhe Complete Mitochondrial Genomes Of Ceratitis Rosa And Ceratitis Quilicii, Members Of The Ceratitis Far Species Complex (Diptera: Tephritidae), Elena Drosopoulou, Aristi Damaskou, Angeliki Markou, Sunday Ekesi, Fathiya Khamis, Aruna Manrakhan, Antonios A. Augustinos, George Tsiamis, Kostas Bourtzis

All Peer-Reviewed Publications

Ceratitis FAR is an African species complex comprising insect pests of great economic interest and obscure species limits. Here, we report the mitochondrial genomes of two members of the FAR complex, namely Ceratitis rosa and the recently characterized Ceratitis quilicii. A phylogenetic analysis based on PCGs of available Tephritidae mitogenomes is presented. The current mitochondrial sequences from the FAR complex could contribute toward the resolution of phylogenetic relationships and species limits within this taxonomically challenging group, which is also an important issue for the development of environment-friendly and species-specific control methods, such as the sterile insect technique (SIT).

The Importance Of Store Hygiene For Reducing Post-Harvest Losses In Smallholder Farmers’ Stores: Evidence From A Maize-Based Farming System In Kenya, Kobia J. Makinya, John M. Wagacha, Judith A. Odhiambo, Paddy Likhayo, Kukom Edoh-Ognakossan, Tadele Tefera, Adebayo Abass, Christopher M. Mutungi

All Peer-Reviewed Publications

Knowledge of the role of hygiene in reducing food loss in farm stores is limited among extensionists, researchers, and farmers. Store hygiene practices during post-harvest handling and storage of maize were assessed using a cross-sectional survey of 342 farmers, with regular follow-up of 40 farmers’ stores over seven months to measure losses caused by insects, and to score the hygiene levels using a standard checklist. Fractional Response Model was used to evaluate the associations between hygiene practices and the losses. Farmers stored their produce in sacks (98.2%) kept in outside granaries (60.1%), or rooms in dwelling houses (39.9%). Co-storage with …

Affordable Processing Of Edible Orthopterans Provides A Highly Nutritive Source Of Food Ingredients, Forkwa Tengweh Fombong, John Kinyuru, Jeremiah Ng’Ang’A, Monica Ayieko, Chrysantus Mbi Tanga, Jozef Vanden Broeck, Mik Van Der Borght

All Peer-Reviewed Publications

Edible orthopterans (grasshoppers, crickets, and locusts) are major delicacies, especially across sub-Saharan Africa. Their promotion as food ingredients is increasingly gaining momentum. This study evaluates the nutritional profiles of three widely consumed orthopterans: Gryllus bimacu-latus, Locusta migratoria, and Schistocerca gregaria after blanching and oven-drying. All three species had high protein (65.3, 54.2, and 61.4% on a dry matter (DM) basis for G. bimaculatus, L. migratoria, and S. gregaria, respectively) and fat contents. Oleic (22.9–40.8%) and palmitic (26.1–43.0%) were the two most abundant fatty acids. All essential amino acids (in mg/100 g protein) were present, with glutamic acid (120–131), alanine (90.2–123), …