Entomology Commons^™

Tomato Chlorosis Virus Infection Facilitates Bemisia Tabaci Med Reproduction By Elevating Vitellogenin Expression, Liping Huang, Xiaobin Shi, Jizhe Shi, Zhuo Zhang, Yong Fang, Zhanhong Zhang, Qiuyi Pan, Limin Zheng, Yang Gao, Deyong Zhang, Xinqiu Tan, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg …

The Use Of Insecticides To Manage The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Leconte: History, Field-Evolved Resistance, And Associated Mechanisms, Lance J. Meinke, Dariane Souza, Blair D. Siegfried

Department of Entomology: Faculty Publications

The western corn rootworm, Diabrotica virgifera virgifera LeConte (Dvv) is a significant insect pest of maize in the United States (U.S.). This paper reviews the history of insecticide use in Dvv management programs, Dvv adaptation to insecticides, i.e., field-evolved resistance and associated mechanisms of resistance, plus the current role of insecticides in the transgenic era. In the western U.S. Corn Belt where continuous maize is commonly grown in large irrigated monocultures, broadcast-applied soil or foliar insecticides have been extensively used over time to manage annual densities of Dvv and other secondary insect pests. This has contributed to the sequential occurrence …

Edible Crickets (Orthoptera) Around The World: Distribution, Nutritional Value, And Other Benefits—A Review, Henlay J.O. Magara, Saliou Niassy, Monica A. Ayieko, Mukundi Mukundamago, James P. Egonyu, Chrysantus M. Tanga, Emily K. Kimathi, Jackton O. Ongere, Komi K.M. Fiaboe, Sylvain Hugel, Mary A. Orinda, Nanna Roos, Sunday Ekesi

All Peer-Reviewed Publications

Edible crickets are among the praised insects that are gaining recognition as human food and livestock feed with a potential of contributing to food security and reduction of malnutrition. Globally, the sustainable use of crickets as food or feed is undermined by lack of information on the number of the edible crickets, the country where they are consumed, and the developmental stages consumed. Furthermore, lack of data on their nutritional content and the potential risks to potential consumers limits their consumption or inclusion into other food sources. We reviewed published literature on edible cricket species, countries where they are consumed, …

Increased Cave Use By Butterflies And Moths: A Response To Climate Warming?, Otto Moog, Erhard Christian, Rudolf Eis

International Journal of Speleology

Between 2015 and 2019, the list of Lepidoptera from “cave” habitats (i.e., proper caves, rock shelters and artificial subterranean structures) in Austria grew from 17 to 62 species, although the effort of data collection remained nearly constant from the late 1970s onwards. The newly recorded moths and butterflies were resting in caves during daytime in the the warm season, three species were also overwintering there. We observed Catocala elocata at 28 cave inspections, followed by Mormo maura (18), Catocala nupta (7), Peribatodes rhomboidaria, and Euplagia quadripunctaria (6). More than half of the species have been repeatedly observed in caves …

Heterocyclic Amine-Induced Feeding Deterrence And Antennal Response Of Honey Bees, Nicholas R. Larson, Scott T. O'Neal, Thomas P. Kuhar, Ulrich R. Bernier, Jeffrey R. Bloomquist, Troy D. Anderson

Department of Entomology: Faculty Publications

The productivity and survival of managed honey bee colonies is negatively impacted by a diverse array of interacting factors, including exposure to agrochemicals, such as pesticides. This study investigated the use of volatile heterocyclic amine (HCA) compounds as potential short term repellents that could be employed as feeding deterrents to reduce the exposure of bees to pesticide-treated plants. Parent and substituted HCAs were screened for efficacy relative to the repellent N,N-diethyl-meta-toluamide (DEET) in laboratory and field experiments. Additionally, electroantennogram (EAG) recordings were conducted to determine the level of antennal response in bees. In video-tracking recordings, bees were observed to spend …

Intragenic Dna Methylation Regulates Insect Gene Expression And Reproduction Through The Mbd/Tip60 Complex, Guanfeng Xu, Hao Lyu, Yangqin Yi, Yuling Peng, Qili Feng, Qisheng Song, Chengcheng Gong, Xuezhen Peng, Subba Reddy Palli, Sichun Zheng

Entomology Faculty Publications

DNA methylation is an important epigenetic modification. However, the regulations and functions of insect intragenic DNA methylation remain unknown. Here, we demonstrate that a regulatory mechanism involving intragenic DNA methylation controls ovarian and embryonic developmental processes in Bombyx mori. In B. mori, DNA methylation is found near the transcription start site (TSS) of ovarian genes. By promoter activity analysis, we observed that 5′ UTR methylation enhances gene expression. Moreover, methyl-DNA-binding domain protein 2/3 (MBD2/3) binds to the intragenic methyl-CpG fragment and recruits acetyltransferase Tip60 to promote histone H3K27 acetylation and gene expression. Additionally, genome-wide analyses showed that the peak …

Up-Regulation Of Apoptotic- And Cell Survival-Related Gene Pathways Following Exposures Of Western Corn Rootworm To B. Thuringiensis Crystalline Pesticidal Proteins In Transgenic Maize Roots, Brad S. Coates, Emeline Deleury, Aaron J. Gassmann, Bruce E. Hibbard, Lance Meinke, Nicholas J. Miller, Jennifer L. Petzold-Maxwell, B. Wade French, Thomas W. Sappington, Blair D. Siegfried, Thomas Guillemaud

Department of Entomology: Faculty Publications

Background: Resistance of pest insect species to insecticides, including B. thuringiensis (Bt) pesticidal proteins expressed by transgenic plants, is a threat to global food security. Despite the western corn rootworm, Diabrotica virgifera virgifera, being a major pest of maize and having populations showing increasing levels of resistance to hybrids expressing Bt pesticidal proteins, the cell mechanisms leading to mortality are not fully understood.

Results: Twenty unique RNA-seq libraries from the Bt susceptible D. v. virgifera inbred line Ped12, representing all growth stages and a range of different adult and larval exposures, were assembled into a reference transcriptome. Ten-day exposures …

Quantitative Assessment Of Aphid Parasitoids And Predators In Central Oklahoma Wheat Fields During Five Growing Seasons, Norman C. Elliott, Kristopher L. Giles, Kristen A. Baum, Sarah Elzay

Biological Sciences Faculty Publications

Winter wheat (Triticum aestivum L.) is the most widely grown crop in Oklahoma and typically is planted in autumn and harvested in June. Wheat in Oklahoma is often infested by insect pests, the most important of which are the cereal aphids – greenbug, Schizaphis graminum (Rondani), and bird cherry-oat aphid, Rhopalosiphum padi (L.). We sampled a total of 69 wheat fields in central Oklahoma during five wheat-growing seasons. The number of wheat fields sampled ranged from seven in the 2016-2017 growing season to 24 fields in the 2009-2010 growing season. We used a D-vac suction sampler to collect aphids …

Soil Macroinvertebrate Responses To Wildfires In The Blue Ridge Mountains, Usa, Madeline Nicole Olliff, Bruce Snyder, Mac Callaham Jr., Melanie K. Taylor

Graduate Research Showcase

Title: Soil Macroinvertebrate Responses to Wildfires in the Blue Ridge Mountains, USA Authors: 1Madeline N. Olliff, 1Bruce A. Snyder, 2Melanie K. Taylor, and 2Mac A. Callaham

1Georgia College and State University, Milledgeville, Georgia, USA; 2USDA Forest Service, Southern Research Station, Athens, Georgia, USA

Abstract: Wildfires are of increasing concern in light of climate change, more frequent late summer droughts, and increasing incidence of human ignitions. There have been few studies examining the effects of wildland fires on soil macroinvertebrates in the Blue Ridge Mountains, in spite of the importance of these animals to soil processes, and their contributions to the …

Nebline, January 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2020 Extension Highlight Highlights Responding as Local Needs Changed

Helping People Solve Real-World Problems

20,311 Youth Engaged in 4-H Programs

Make Your New Year’s Resolutions Stick

Recipe of the Month

Crop Production Clinics, Jan. 7, 14, and 21

Pesticide Applicator Trainings

Extension’s 2021 Master Gardener Trainings, Two Sites Available

Upcoming Green Industry Conferences

Garden Guide Things to do This Month

HEART OF 4-H VOLUNTEER AWARD: Emalea Dean

PASE Results

4-H Thanks Sponsors

4-H Announcements for 4-H’ers and Volunteers

4-H Thanks Volunteers

EXTENSION CALENDAR

EXTENSION NEWS

Dung Beetle (Scarabaeinae) Diversity Of The Highest Elevation In West Africa: The Nimba Mountain Range, Jacob Bowen

Mahurin Honors College Capstone Experience/Thesis Projects

The Nimba Mountain Range in Guinea, Ivory Coast, and Liberia is within the Upper Guinean Forests, a critical biodiversity hotspot highly threatened by various human activities. The region is home to many endemic species including the viviparous Nimba toad, Nimba otter-shrew, and the discrete Bossou chimpanzee population. Dung beetles can act as a focal taxon from which extrapolation to the diversity of other taxa and ecosystem health can be made. Elevational trends in dung beetle diversity were investigated on the Nimba Mountain Range and in the nearby Bossou Chimpanzee reserve in Guinea. Dung beetle species diversity surveys aimed to document …

A Comparison Of Bee Abundance And Species Richness In Three Managed Grassland Types, Emily Russ

Mahurin Honors College Capstone Experience/Thesis Projects

Bees are threatened by environmental changes, pathogens, and pesticides (Pettis, 2012, Meeus et al., 2018). The goal of the study is to compare and evaluate the bee abundance and species richness in three different field types. My hypothesis is that bees would favor the fields planted for their benefit, and that the tall grass dominated plantings would be preferred over mowed fescue. My findings have low power considering few replicates and the use of relative abundance and relative species richness for statistical analyses. Bee abundance and species richness had an overall positive relationship of varying degrees across habitat types. Relative …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

A Review And Comparison Of U.S. State Wildlife Action Plans For Stonefly (Insecta, Plecoptera) Species Of Greatest Conservation Need, Kathryn Greene

Mahurin Honors College Capstone Experience/Thesis Projects

State Wildlife Action Plans (SWAPs) were created in order to identify Species of Greatest Conservation Need (SGCN) and develop plans to prevent their populations from further decline and the possibility of being listed as threatened or endangered. Which SGCNs are included are decided based on characteristics that determine their vulnerability. As of early 2021, approximately 720stoneflies (Insecta, Plecoptera) species are found in the U.S., but only 143 are listed as SGCN. Only 29 states have stoneflies included on their SGCN lists, but 37 states use EPT (Ephemeroptera + Plecoptera + Trichoptera) metrics when assessing water quality issues in running water …

Learn The Beetle And Discover The Blue, Nicole Luhr

Department of Entomology: Distance Master of Science Projects

Objectives: For children to: Learn basic body parts of a beetle and put their own together./ Observe and relate body parts of a beetle to a live beetle./ Determine how humidity levels affect the color of beetles.

Contents: 1 Lesson Overview 3 Lesson Instruction 2 Insect Background 4 - 5 Activity #1 6 - 7 Blue Death Feigning Beetle Info and Care 1 1 - 1 9 Activity #3 8 - 1 0 Activity #2 2 0 Glossary and References

Butterfly Life Cycle: Curriculum, Lesson Plans And Resources, Matthew D. Trujillo

Department of Entomology: Distance Master of Science Projects

This curriculum and guide contains lessons, each with exercises and activities, a science-based word bank and glossary, and Missouri content standards and descriptions. This is a centers-based curriculum intended to be used in conjunction with a science center such as the Butterfly Palace or Butterfly House. There are three major themes in this curriculum: The Life Cycle of Butterflies, Butterfly Habitats, and Adaptations for the Units. This curriculum is unique in that each lesson can serve as a stand alone which can be taught in a classroom whether home based, or school based and does not require sequencing with the …

Mosquitoes Of Wisconsin: The Biology, Life History, Identification And Control, Timothy Wucherer

Department of Entomology: Distance Master of Science Projects

In the past 60 years, weather in Wisconsin has generally been getting warmer, and in many areas of the state, wetter (Moran et al., 2015). Long-term analysis of Wisconsin’s climate has indicated an increase in average annual precipitation, longer growing seasons, and an increase in average temperatures (Moran et al., 2015). Increases in temperatures and rain have been directly correlated with increases in vector-borne diseases such as pathogens carried by mosquitoes (Moran et al., 2015). With this, Wisconsin communities should prepare for possible increases in mosquito activity and the health and veterinary concerns that come with direct exposure to certain …

Wa Insecticide Guide 2021 - Autumn Winter, Amber Balfour-Cunningham, Dusty Severtson

PestFacts WA

Registered chemicals for crops during autumn and winter 2021 in Western Australia

Wa Insecticide Guide 2021 - Winter Spring, Svetlana Micic, Alan Lord

PestFacts WA

Registered chemicals for crops during winter and spring 2021.

The pesticides listed in the tables below can be used on any crop appearing on the chemical label, as long as the rate used does not exceed the highest rate that is registered for use on that crop.

There are many products with different trade names that contain the same active ingredient. This list is not exhaustive and does not imply any specific recommendations of brand names.

Unless otherwise specified all insecticides listed are emulsifiable concentrate (EC). Insecticides with SC are suspension concentrate, WP is wettable powder, CS is capsule suspension, …

Bedbug Education, Treatment, And Prevention, Elizabeth Bedford

Department of Entomology: Distance Master of Science Projects

Cimex lectularius (bedbug) has become a severe issue in more recent years due to travel, immigration, and importation of products. Unfortunately, most people do not know their biology hence do not treat these arthropods correctly. Many myths and misinformation are currently available to people on the internet and with pest professionals. Education and proper treatment must eradicate these pests in homes, commercial buildings, and other places. To prove whether or not education with treatment garners better results,ten homes were treated with the same heating and chemical process, with only five being educated before, during and after. All homes were inspected …

Macroinvertebrate Responses To Hydrological Variation In Experimental Wetlands., Sergio A. Sabat-Bonilla

College of Graduate Studies: Theses & Dissertations

Predicted increases in the frequency of intense storms and periods of severe drought due to climate change represent a threat to wetland macroinvertebrate communities through alterations to the hydrological regime. I used experimental ponds to assess the effects of water permanence (i.e., duration of flooding) on the communities of aquatic macroinvertebrates. I predicted that permanent ponds would harbor higher diversity of longer-lived taxa whereas temporary ones will favor colonization by quick turnover, short-lived taxa and support lower consumer diversity. Results show differences in macroinvertebrate communities between permanent and temporary ponds can be mostly explained by hydrology and the amount of …

Evaluation Of Biotype 4 Aphis Glycines (Hemiptera: Aphididae) Induced Susceptibility On Soybean And Soja, Jakob David Hicks

Electronic Theses and Dissertations

Soybean aphids have been a significant pest of soybeans in North America since 2000. Before 2000, soybeans did not face significant insect pest pressure from any arthropods with piercing-sucking mouthparts. It is estimated that economic damage from soybean aphids range from $1billion to $4.7 billion annually. Research efforts focused on the identification of host plant resistance genes in soybean and discovered many resistant to Aphis glycines genes (i.e., Rag genes) in soybean. However, the adoption of commercially released Rag soybean cultivars has been limited. The prospect of management with Rag genes was further complicated by the identification of three virulent …

Letter From The Dean, Deacue Fields

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 22 2021, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik

Murray State Theses and Dissertations

Hatchery Creek is a restored stream in Jamestown, KY that drains Wolf Creek National Fish Hatchery. The previous degraded channel of Hatchery Creek caused large sediment plumes in the Lower Cumberland River and was restored to decrease sediment loss and provide the opportunity for a self-sustaining trout population. I predicted that the increased amount of habitat would increase taxa richness, abundance and biomass of macroinvertebrates. Macroinvertebrates were monitored in three periods; the degraded period, a one-year recovery period, and a restored period using surber and kicknet samples in order to determine biomass, abundance, diversity, taxa richness, and macroinvertebrate biotic index. …

Use Of Lymesim 2.0 To Assess The Potential For Single And Integrated Management Methods To Control Blacklegged Ticks (Ixodes Scapularis; Acari: Ixodidae) And Transmission Of Lyme Disease Spirochetes, Shravani Chitineni, Elizabeth R. Gleim, Holly D. Gaff

Undergraduate Honors Theses

Annual Lyme disease cases continue to rise in the U.S. making it the most reported vector-borne illness in the country. The pathogen (Borrelia burgdorferi) and primary vector (Ixodes scapularis; blacklegged tick) dynamics of Lyme disease are complicated by the multitude of vertebrate hosts and varying environmental factors, making models an ideal tool for exploring disease dynamics in a time- and cost-effective way. In the current study, LYMESIM 2.0, a mechanistic model, was used to explore the effectiveness of three commonly used tick control methods: habitat-targeted acaricide (spraying), rodent-targeted acaricide (bait boxes), and white-tailed deer targeted acaricide (4-poster …

High Plains Wheat Mosaic Virus: An Enigmatic Disease Of Wheat And Corn Causing The High Plains Disease, Satyanarayana Tatineni, Gary Hein

Department of Entomology: Faculty Publications

Brief history: In 1993, severe mosaic and necrosis symptoms were observed on corn (maize) and wheat from several Great Plains states of the USA. Based on the geographical location of infections, the disease was named High Plains disease and the causal agent was tentatively named High Plains virus. Subsequently, researchers renamed this virus as maize red stripe virus and wheat mosaic virus to represent the host and symptom phenotype of the virus. After sequencing the genome of the pathogen, the causal agent of High Plains disease was officially named as High Plains wheat mosaic virus. Hence, High Plains virus, …

Landscape Characterization Of Floral Resources For Pollinators In The Prairie Pothole Region Of The United States, Autumn H. Smart, Clint R.V. Otto, Alisa L. Gallant, Michael P. Simanonok

Department of Entomology: Faculty Publications

Across agricultural areas of the Prairie Pothole Region (PPR), floral resources are primarily found on public grasslands, roadsides, and private grasslands used as pasture or enrolled in federal conservation programs. Little research has characterized the availability of flowers across the region or identified the primary stakeholders managing lands supporting pollinators. We explored spatial and temporal variability in flower abundance and richness across multiple grassland categories (i.e. general grassland, conservation grassland, and engineered pollinator habitat) in the PPR from 2015 to 2018 and used these data to estimate the number of flowering stems present across the region on private and public …

Characterizing The Relationship Between Western Corn Rootworm (Coleoptera: Chrysomelidae) Larval Survival On Cry3bb1-Expressing Corn And Larval Development Metrics, Jordan D. Reinders, David S. Wangila, Emily Robinson, B. Wade French, Lance Meinke

Department of Entomology: Faculty Publications

The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a significant pest of field corn, Zea mays L. (Poales: Poaceae), across the United States Corn Belt. Widespread adoption and continuous use of corn hybrids expressing the Cry3Bb1 protein to manage the western corn rootworm has resulted in greater than expected injury to Cry3Bb1-expressing hybrids in multiple areas of Nebraska. Single-plant bioassays were conducted on larval western corn rootworm populations to determine the level of resistance present in various Nebraska counties. The results confirmed a mosaic of susceptibility to Cry3Bb1 across Nebraska. Larval development metrics, including head capsule width …