Biochemistry Commons^™

Ascorbic Acid, Lipid Peroxidation, And Aging, Brian Evan Leibovitz

Dissertations and Theses

The role of ascorbic acid with regard to lipid peroxidation and aging has been examined. A thorough literature analysis indicates that free radical-induced lipid peroxidation is a plausible biochemical explanation for aging. Lipid peroxidation causes cellular damage due to altered enzyme activities, error-prone nucleic acid metabolism, and membrane dysfunction, as well as the accumulation of aging pigments in the lysomes. Ascorbic acid, a water soluble free radical quencher, was examined with regard to carbon tetra-chloride-induced lipid peroxidation and in vivo aging. Carbon tetrachloride, a well-known free radical inducer, caused marked increases in the ration of oxidized/reduced vitamin C only …

Steriodogenesis In Mouse Preimplantation Embryos, Marcia E. Kendall

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Marcia E. Kendall February 8, 1979.

Potential Use Of Hydroelectric Facilities For Manipulating The Fertility Of Lake Mead, Larry J. Paulson, John R. Baker, James E. Deacon

Publications (WR)

Analysis of historical nutrient data for Lake Mead indicates that the fertility of the reservoir has decreased which may be the cause for a corresponding decline in the largemouth bass population. However, it appears that fertility can be manipulated by altering the operation of the dam. The depletion of nutrients in the euphotic zone by phytoplankton and subsequent accumulation in the hypolimnion during summer and fall provide a natural nutrient gradient from which water of varying fertility can be drawn for discharge. This combined with alterations in the depth or seasonal pattern of discharge can possibly be used to enhance …

Limnological Aspects Of Lake Mead, Nevada-Arizona, John R. Baker, James E. Deacon, Thomas A. Burke, Samuel S. Egdorf, Larry J. Paulson, Richard W. Tew, Bureau Of Reclamation

Publications (WR)

Lake Mead is a deep, subtropical, moderately productive, desert impoundment with a negative heterograde oxygen profile occurring during; the summer stratification. investigations of the Boulder Basin of Lake Mead by the University of Nevada were initiated in November 1971. The primary objective of the study was to determine what effects industrial and sewage effluent from the Las Vegas metropolitan area, discharged into Las Vegas Bay, have had on the water quality and limnological conditions of Boulder Basin. Data from the 1975-76 period are presented in detail, with earlier data included in the summaries and discussions.

Measurements of water temperature, dissolved …

Distribution Of Stream Pollution In Lake Water, Richard W. Tew, Samuel S. Egdorf, James E. Deacon

Publications (WR)

Wastewater effluent-laden waters from Las Vegas Wash (LVW) form a density current that may be detected in Boulder Basin of Lake Mead at considerable distances from the wash estuary. This led to the suspicion that water from the inflowing stream [40 mgd (1.5 X 10⁵ cu m/day)] might not be rapidly diluted in the enormous volume of the lake [19 mil acre-ft (2.3 X 10¹⁰ cu m)], but might persist as a recognizable entity to the vicinity of the intake of a major water source for populous Clark County, Nev.

Because of the detection sensitivity implicit in the …

The Role Of Zinc In Dihydroorotase, Pamela S. Gilchrist

Dissertations and Theses

Dihydroorotase (4,4—dihydroorotic acid amidolyase, EC 3.5.2.3.) which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate has been purified from orotate-grown Clostridium oroticum. The enzyme is stable in 0.3 M sodium chloride and 10 µ ZnSO₄. Sodium dodecyl sulfate gel electrophoresis indicates the enzyme to be composed of two identical subunits each with a molecular weight of 58,000 + 6000. Dihydroorotase is shown to be a zinc-containing metalloenzyme with 2 g atoms of zinc per 58,000 g of protein. The role of zinc in dihydroorotase is discussed.

Seasonal And Spatial Variation In Primary Productivity In Boulder Basin, Lake Mead, Clark County, Nevada, Isamu Aoki

Publications (WR)

The ¹⁴C light and dark bottle technique for measurement of primary production was utilized as a means of assessing the amount of inorganic carbon being converted Into organic form by the photosynthesis of phytoplankton populations In the Boulder Basin of Lake Mead.

Spatial and time series changes of productivity levels observed at eight sampling locations within Boulder Basin Indicate that the Influence of treated municipal arts' industrial effluent flowing into Les Vegas Bay is contributing high levels of available nutrients at Las Vegas Wash Inlet to cause productivity to approximate those levels associated with polluted waters.

Productivity levels at …

A Study Of Rna Bacteriophage 7s Infection Of Pseudomonas Aeruginosa, Deanne Benson

Dissertations and Theses

A study was conducted to find the effect of magnesium, calcium, manganese and zinc ions on the infection of Psudomonas aeruginosa strain 1C by RNA bacteriophage 7s. When an 18 hour progeny experiment was performed, it was found that magnesium, calcium and manganese had different effects on bacteriophage production and was dependent on the bacterial growth conditions. RNA bacteriophage 7s progeny production was significantly enhanced by the addition of magnesium to cultures of Psudomonas aeruginosa 1C grown in a magnesium deficient medium. Under these environmental conditions there was a slight increase in progeny in the presence of calcium. When Psudomonas …

Enzyme Reactions Using Ureidosuccinate As A Substrate During Pyrimidine Biosynthesis And Degradation In Cl. Oroticum, Penny Amy

Dissertations and Theses

Cells of Clostridium oroticum, an anaerobic bacterium, were grown on orotate as a carbon and energy source. Ureidosuccinase, an inducible enzyme in the pathway for pyrimidine degradation has been shown to convert ureidosuccinate to aspartate, CO₂ and NH₃ as reported by Liebenmm and Kornberg (7). Aspartate and CO₂ were formed in approximately a 1:1 ratio from ureidosuccinase activity.

Ureidosuccinase was found to be a Mn+² requiring enzyme with a pH optimum of approximately pH 6.5. Enzyme activity is labile to O₂, temperature, pH, dilution and high ionic strength. The optimum conditions for storage …

A Diurnal Zooplankton Migration Study In Lake Mead, Robert D. Staker

Publications (WR)

The diurnal vertical movement of zooplankton was first recorded in freshwater lakes by Weismann (1877) in Lake Constance (Bodensee), although Cuvier was credited with observing the migration of Daphniae in 1817 (Gushing, 1955). The migration is best observed in deep oligotrophic lakes and migrations of 50 m per day are described (Worthington, 1931). In addition, two species of chaetognaths are reported to migrate 400 m a day in marine waters off of Lisbon (Waterman and Berry, 1967) while Birge (1895) found little evidence of the phenomenon at all in Lake Mendota, Wisconsin.

Most zooplankton that migrate rise at night and …

Final Report On Interrelationships Between Chemical, Physical And Biological Conditions Of The Waters Of Las Vegas Bay Of Lake Mead, James E. Deacon, Richard W. Tew

Publications (WR)

This program was a status study of the interaction between Las Vegas Wash, an enriched stream, and Las Vegas Bay, a wedge shaped arm of one of the world's deeper reservoirs. The program centered primarily on identification and counting of planktonic algae from several points in Las Vegas Bay. Additional work on nutrient enrichment of water samples was conducted to aid in interpretation of algal distribution related to nutrient input. Examination of a variety of physical, chemical, and biological parameters, both at many surface points in the bay, as well as in vertical profile, was also accomplished and further aided …

A Conceptual Draft Of A Dynamic Hydro-Biological Model For Lake Mead, L. G. Everett, Bureau Of Reclamation

Publications (WR)

Food and energy transformation in an aquatic system must be understood in order to develop a simulation approach. This report provides a comprehensive study of an aquatic ecosystem. The objectives of this report are to show:

(1) the biological relationships in an aquatic system

(2) the role of nutrients in the biological cycle

(3) the role of abiotic factors in a limnetic environment

(4) the status of the art of "Eutrophication modeling".

Micronutrients And Biological Patterns In Lake Mead, Hasan K. Qashu, Lorne G. Everett, J. S. Carlson, Bureau Of Reclamation

Publications (WR)

Progressive increases in concentration of dissolved solids in the Colorado River water from Lake Powell to Imperial Dam seem to alter plankton dynamics and biological productivity of the river. Also, changes in biological productivity and micronutrients concentrations occur within the same reservoir. Development of a digital simulation model to predict micronutrients concentrations and biological productivity is necessary for diagnosing changes in plankton population and effluent-carrying capacity of the system.

The objectives of the study are: (1) to determine trace metal balance at different locations in Lake Mead, (2) to measure biological productivity and conduct plankton population counts at each sampling …

Purification And Properties Of Triosephosphate Isomerase From Selected Chlorophyta, John C. Meeks

All Master's Theses

It is the purpose of this study to purify and investigate some of the properties of triosephosphate isomerase from two unicellular green algae 1 Ankistrodesmus Braunii and Scenedesmus acuminatus, and one filamentous green alga, Chara sp.

Comprehensive Survey Of Sedimentation In Lake Mead, 1948-49, W. O. Smith, C. P. Vetter, G. B. Cummings, U.S. Bureau Of Reclamation

Publications (WR)

Reservoirs are becoming an increasingly prominent feature of the American landscape. Built for flood mitigation and to change a fluctuating river into a dependable source of water for irrigation, power, and other purposes, they are predestined, like natural lakes, to be destroyed sometime following their creation. Sedimentation sooner or later robs most lakes and reservoirs of their capacity to store water. The significance of sedimentation in the life of Lake Mead, the largest artificial reservoir in the world, was realized when the plan for the reservoir was conceived, and an aerial survey of the floor was made in 1935 before …

The Natural Factors In The Selection Of Diet., Lamar William Neblett

Electronic Theses and Dissertations

No abstract provided.