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Biochemistry Commons

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Full-Text Articles in Biochemistry

Dietary Protein Deficiency And Mycobacterium Bovis Bcg Affect Interleukin-2 Activity In Experimental Pulmonary Tuberculosis, David N. Mcmurray, C. L. Mintzer, R. A. Bartow, Rebecca D. Parr Sep 1989

Dietary Protein Deficiency And Mycobacterium Bovis Bcg Affect Interleukin-2 Activity In Experimental Pulmonary Tuberculosis, David N. Mcmurray, C. L. Mintzer, R. A. Bartow, Rebecca D. Parr

Faculty Publications

Inbred strain 2 guinea pigs were vaccinated with Mycobacterium bovis BCG or were left unvaccinated. They were maintained for 6 weeks on defined, isocaloric diets containing either 30% (control animals) or 10% (animals receiving low protein) ovalbumin as the sole protein source. Animals were challenged by the respiratory route with a low dose of virulent M. tuberculosis H37Rv and killed 4 weeks later. Protein-malnourished animals were not protected by previous vaccination with BCG. Lymphocytes isolated from various tissues were tested in vitro for proliferative responses to mitogen (concanavalin A) and antigen (purified protein derivative [PPD]), production of interleukin-2 (IL-2), and …


Limnological Monitoring Data For Lake Mead During 1988, Suzanne E. Leavitt, Larry J. Paulson, State Of Nevada: Division Of Environmental Protection Apr 1989

Limnological Monitoring Data For Lake Mead During 1988, Suzanne E. Leavitt, Larry J. Paulson, State Of Nevada: Division Of Environmental Protection

Publications (WR)

Limnological monitoring was conducted in Las Vegas Bay and Boulder Basin from April to December of 1988. The purpose of the monitoring was to (i) document possible changes in water quality resulting from decreased phosphorus loading and increased ammonia in Las Vegas Wash, and (ii) establish a data base for evaluating the adequacy of water quality standards.


Expression Of Holo And Apo Forms Of Spinach Acyl Carrier Protein-I In Leaves Of Transgenic Tobacco Plants, M. A. Post-Beittenmiller, Katherine M. Schmid, J. B. Ohlrogge Jan 1989

Expression Of Holo And Apo Forms Of Spinach Acyl Carrier Protein-I In Leaves Of Transgenic Tobacco Plants, M. A. Post-Beittenmiller, Katherine M. Schmid, J. B. Ohlrogge

Scholarship and Professional Work - LAS

Acyl carrier protein (ACP) is a chloroplast-localized cofactor of fatty acid synthesis, desaturation, and acyl transfer. We have transformed tobacco with a chimeric gene consisting of the tobacco ribulose-1,5-bisphosphate carboxylase promoter and transit peptide and the sequence encoding the mature spinach ACP-I. Spinach ACP-I was expressed in the transformed plants at levels twofold to threefold higher than the endogenous tobacco ACPs as determined by protein immunoblots and assays of ACP in leaf extracts. In addition to these elevated levels of the holo form, there were high levels of apoACP-I, a form lacking the 4'-phosphopantetheine prosthetic group and not previously detected …