Animal Sciences Commons^™

Effectiveness Of Pathogen-Specific Passive Antibodies To Mitigate Infectious Diseases In Apis Mellifera, Tanner Nordseth

Honors Thesis

Honeybees (Apis mellifera) are widely recognized as a vital part of the global ecosystem and the world's food supply due to their pivotal role in the pollination of both natural and agricultural flora. Colony Collapse Disorder (CCD) is an emerging phenomenon characterized by a colony's worker bees deserting the hive and leaving the queen behind. This usually results in colony failure. CCD is a multifactorial issue, with many environmental stressors and pathogens playing a role. Deformed Wing Virus (DWV) has been identified as a leading cause in this phenomenon. Paenibacillus larvae (P. larvae) is another lethal pathogen that is …

Growing South Dakota (Spring 2021), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2020 SDSU Extension Annual Report and 2020 South Dakota Agricultural Experiment Station Annual Report.

[Page] 3 COVID-19 Impacts
[Page] 9 Precision Agriculture: What's New
[Page] 13 Featured Research
[Page] 19 SDSU Extension 2020 Annual Report
[Page] 45 South Dakota Agricultural Experiment Station 2020 Annual Report
[Page] 55 CAFES News and Updates
[Page] 67 Alumni News
[Page] 75 Alumni Gone Down in History
[Page] 81 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Changes In Whole Blood Parameters In Beef Heifers May Contribute To Delayed Pubertal Attainment, Jessica A. Keane

Department of Animal Science: Dissertations, Theses, and Student Research

Pubertal development in beef heifers is a transient period that has many contributing factors. Previously in our lab, progesterone (P4) concentrations collected from heifers at weaning (Oct) to breeding (May) were used to identify four distinct pubertal groups: Typical, Early, Start-Stop, and Non-Cycling. There is limited research on heifers and the impacts of whole blood cell populations, particularly WBCs, during pubertal development; however, girls with differing puberty onset displayed altered concentrations of hematocrit, white blood cells (WBC), and hemoglobin concentrations. Thus, our hypothesis was that heifer pubertal groups with delayed or precocious puberty may have increased white blood cells or …

Variation In The Genome And Transcriptome Associated With Beef Cattle Production And Investigation Of The Metabolic Consequences Of Beta-Adrenergic Agonist Supplementation, Renae L. Sieck

Department of Animal Science: Dissertations, Theses, and Student Research

Beta-adrenergic agonists (β-AA) are widely used supplements in livestock production to improve feed efficiency and increase lean muscle mass. Heat stress is one of the largest economic burdens to the livestock industry due to production efficiency losses and morbidity and mortality of animals. Both β-AA and catecholamines released in response to heat stress bind to β-adrenoceptors on the skeletal muscle cell surface to activate downstream signaling pathways. The purpose of this study was to determine if β-AA supplementation and heat stress have an additive effect on the skeletal muscle transcriptome. 3’ RNA sequencing of samples of the longissimus dorsi was …

The Effects Of Different Feed Supplements On Performance Parameters, Egg Measurements, And Eggshell Integrity In Older White Leghorn Laying Hens, Josephine N. Foley

Department of Animal Science: Dissertations, Theses, and Student Research

Two studies were conducted to evaluate the efficacy of multiple feed additives on the performance of older White Leghorn hens. Study 1 examined the effects of 2 vitamin/mineral supplements and 1 medium chain fatty acid. Study 2 examined the effects of a novel butyric acid product, a tributyrin ester. Both studies followed a completely randomized design with repeated measures and data were analyzed using the Glimmix procedure in SAS version 9.4 for Windows.

The first study took place November 2015 to April 2016. Trt 1 was a control diet, Trt 2 was supplemented with a Ca, Fe, Mn, Cu, Zn …

Evaluation Of Condensed Algal Residue Solubles As An Ingredient In Cattle Finishing Diets And Its Effects On Digestibility And Fatty Acid Flow And A Comparison Of Single And Dual Implant Strategies In Finishing Heifers, John Calvin Gibbons

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments, a performance study (Exp. 1) and digestion study (Exp. 2) were conducted to evaluate a novel liquid feed, condensed algal residue solubles (CARS), in feedlot diets. In Exp. 1 and Exp. 2, steers were fed three inclusions of CARS at 0%, 2.5%, 5%. There were quadratic responses for greater carcass adjusted final BW, ADG, G:F, HCW, 12^th rib fat, and yield grade. There was a linear increase in marbling score and a linear decrease in DMI and LM area as CARS increased. In Exp. 2, CARS did not affect intake or digestibility. Fatty acid flow at the …

Evaluation Of Three Formulations Of Essential Oils In Broiler Chickens Under Cyclic Heat Stress, Jared Ruff, Guillermo Tellez Jr., Aaron Forga, Roberto Señas-Cuesta, Christine N. Vuong, Elizabeth S. Greene, Xochitl Hernandez-Velasco, Alvaro J. Uribe, Blanca C. Martinez, Jaime A. Angel-Isaza, Sami Dridi, Clay J. Maynard, Casey M. Owens, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The objective of the present research was to assess the dietary supplementation of three formulations of essential oils (EO) in chickens under heat stress (HS). Day-of-hatch Cobb 500 chicks (n = 500) were randomly distributed into four groups: 1. HS control + control diets; 2. HS + control diets supplemented with 37 ppm EO of Lippia origanoides (LO); 3. HS + control diets supplemented with 45 ppm LO + 45 ppm EO of Rosmarinus officinalis (RO) + 300 ppm red beetroot; 4. HS + 45 ppm LO + 45 ppm RO + 300 ppm natural betaine. Chickens that received …

Research Note: Application Of An Escherichia Coli Spray Challenge Model For Neonatal Broiler Chickens, C. M. Selby, B. D. Graham, L. E. Graham, K. D. Teague, B. M. Hargis, G. Tellez-Isaias, C. N. Vuong

Poultry Science Faculty Publications and Presentations

Avian pathogenic Escherichia coli (E. coli) is an opportunistic pathogen often introduced to neonatal chicks during the hatching process. This commensal bacterium, particularly as a pioneer colonizer of the gastrointestinal tract, can have substantial implications in the rearing of poultry because of reduced flock performance. In order to mimic the effects of the natural bacterial bloom present during the hatch, a seeder challenge model was developed to expose neonatal chicks to virulent E. coli. On day 20 of embryogenesis, selected early hatched chicks (n = 18/hatcher) were briefly removed and sprayed challenged with saline (vehicle) or E. …

The Effect Of Feeding Adequate Or Deficient Vitamin B6 Or Folic Acid To Breeders On Methionine Metabolism In 18-Day-Old Chick Embryos, J. Lu, J. T. Weil, P. Maharjan, M. K. Manangi, S. Cerrate, C. N. Coon

Poultry Science Faculty Publications and Presentations

Three isotopic tracers ([2,3,3-²H₃]-L-serine, [²H₁₁]-L-betaine, and [1-¹³C]-L-methionine) were administered by amnion injection into 18-day-old chick embryos to investigate the kinetics of methionine metabolism. The embryos utilized were from eggs collected from 34-week-old Cobb 500 broiler breeders that were fed either a control diet containing folic acid (1.25 mg/kg diet) and pyridoxine HCl (5 mg/kg diet) or diets devoid of supplemental pyridoxine or folic acid. Intermediate metabolites of methionine metabolism and polyamines were analyzed in 18-day-old chick embryos. There were no differences in hepatic [²H₂] methionine or …

Use Of Image Analysis To Identify Woody Breast Characteristics In 8-Week-Old Broiler Carcasses, Juan P. Caldas-Cueva, A. Mauromostakos, X. Sun, Casey M. Owens

Poultry Science Faculty Publications and Presentations

Woody breast (WB) condition causes significant economic losses to the global poultry industry, and the lack of an objective and fast tool to identify this myopathy is a contributing factor. The aim of this study was to determine if there are broiler carcass conformation changes that can be used to identify WB characteristics using image analysis. Images of 8-wk-old male broiler carcasses (n = 544) of high breast-yielding strains were captured before evisceration, which were processed and analyzed using ImageJ software. Measurements were as follows: M0, breast length; M1, breast width in the cranial region; M2, one-fifth of …

Detection Of Woody Breast Condition In Commercial Broiler Carcasses Using Image Analysis, Juan P. Caldas-Cueva, A. Mauromostakos, X. Sun, Casey M. Owens

Poultry Science Faculty Publications and Presentations

Image analysis could be an objective and rapid method to identify woody breast (WB) myopathy and benefit the global poultry industry. The objective of this study was to determine if there are conformational changes that can be used to detect WB characteristics in commercial broiler carcasses across strains, gender, and ages using image analysis. A total of 900 images of male and female broiler carcasses from commercial standard and high breast-yielding strains and 5 ages (6 through 10 wk) were captured before evisceration. These images were processed and analyzed using ImageJ software. Conformational measurements were M0: breast length; …

Population Dynamics And Dispersal Coalitions In The Cooperatively Breeding Acorn Woodpecker, Natasha Hagemeyer

Biological Sciences Theses & Dissertations

Dispersal is a critical life-stage with consequences not only for the individual, but for population dynamics and thus the fate of the whole species. The creation of dispersal coalitions can lead to complex outcomes and affect how changes in abundance occur on the landscape. I examined population dynamics and dispersal coalitions in a population of cooperatively breeding acorn woodpeckers in central California, USA. Using a 34-year dataset on occupancy and abundance, I determined that increases in abundance were determined by dispersal to new locations. This resulted in increased occupancy, rather than increases in group size and larger coalitions. I then …

Assessment Of Lippia Origanoides Essential Oils In A Salmonella Typhimurium, Eimeria Maxima, And Clostridium Perfringens Challenge Model To Induce Necrotic Enteritis In Broiler Chickens, Makenly E. Coles, Aaron J. Forga, Roberto Señas-Cuesta, Brittany D. Graham, Callie M. Selby, Alvaro J. Uribe, Blanca C. Martinez, Jaime A. Angel-Isaza, Christine N. Vuong, Xochitl Hernandez-Velasco, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction …

Unprecedented Migratory Bird Die-Off: A Citizen-Based Analysis On The Spatiotemporal Patterns Of Mass Mortality Events In The Western United States, Di Yang, Anni Yang, Jue Yang, Rongting Xu, Han Qiu

United States Department of Agriculture Wildlife Services: Staff Publications

Extensive, severe wildfires, and wildfire-induced smoke occurred across the western and central United States since August 2020. Wildfires resulting in the loss of habitats and emission of particulate matter and volatile organic compounds pose serious threatens to wildlife and human populations, especially for avian species, the respiratory system of which are sensitive to air pollutions. At the same time, the extreme weather (e.g., snowstorms) in late summer may also impact bird migration by cutting off their food supply and promoting their migration before they were physiologically ready. In this study, we investigated the environmental drivers of massive bird die-offs by …

Standardized Ileal Digestibility Of Amino Acids In Nacedero (Trichanthera Gigantea (Humboldt & Bonpland.) Nees) Foliage, Copra Expeller, And Soybean Meal Fed To Growing Philippine Native Pigs (Sus Scrofa Domesticus Erxleben, 1777), Mary Arnel D. Garcia, Rommel C. Sulabo

The Philippine Agricultural Scientist

The experiment was conducted to determine the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in nacedero (Trichanthera gigantea Humboldt & Bonpland.) Nees; NAC) foliage, copra expeller (CE), and soybean meal (SBM) fed to growing Philippine native pigs (Sus scrofa domesticus Erxleben, 1777). Eight barrows of Black Tiaong strain (initial BW of 36.44 ± 1.59 kg; 132 to 135 d) installed with a T-cannula at the distal ileum were housed individually in metabolism cages and randomly allotted to a replicated 4 × 3 Youden square design with four diets and three periods. One …

Genetic Characterization Of Nodular Worm Infections In Asian Apes, Erhan Yalcindag, Peter Stuart, Hideo Hasegawa, Adrian Streit, Jana Doležalová, Helen Morrogh-Bernard, Susan M. Cheyne, Wisnu Nurcahyo, Ivona Foitová

Department of Biological Sciences Publications

Parasitic nematodes of Oesophagostomum spp., commonly known, as 'nodular worms' are emerging as the most widely distributed and prevalent zoonotic nematodes. Oesophagostomum infections are well documented in African non-human primates; however, the taxonomy, distribution and transmission of Oesophagostomum in Asian non-human primates are not adequately studied. To better understand which Oesophagostomum species infect Asian non-human primates and determine their phylogeny we analysed 55 faecal samples from 50 orangutan and 5 gibbon individuals from Borneo and Sumatra. Both microscopy and molecular results revealed that semi-wild animals had higher Oesophagostomum infection prevalence than free ranging animals. Based on sequence genotyping analysis targeting …

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic …

Understanding Relationships Between Stress, Snake Fungal Disease, And Parasitism In Wild Cottonmouth Snake Populations, Emma Fehlker Campbell

Scholars Week

Chronically raised stress levels are known to correlate with decreased immune function in vertebrates, possible leading to increased susceptibility to infections including parasitism and Snake Fungal Disease (SFD). Helminth endoparasites and Hemoparasites are frequently found in wild populations but little is known about their physiological effects. Additionally, parasite infection may have interactive effects with diseases including SFD. To our knowledge few studies have assessed potential interactions between SFD and parasites in snakes. Thus, the objective of our study is to assess the individual and interactive effects of Helminth endoparasites, hemoparasites, and snake fungal disease on cottonmouth physiological stress. We are …

Lymphocyte Soluble Factors From Pregnant Cows Modulate Mrna Transcript Abundances Encoding For Proteins Associated With Trophoblast Growth And Development, Kelsy A. Leppo, Preston Collins, Kira Morgado, Ana C. Silva, Aaron Thomas, Heloisa M. Rutigliano

Animal, Dairy, and Veterinary Science Faculty Publications

This study was conducted to determine whether T cell populations are responsible for modulating placental development during gestation in cattle. It was hypothesized that CD4+CD25+ and γ/δ+ T cells modulate gene expression, based on mRNA transcript abundances, and promote proliferation and survival of trophoblast cells. Peripheral blood was collected from cows at 160 to 180 days of gestation and non-pregnant cows, T cell populations CD8+, CD4+, CD4+CD25+, CD24+CD25-, and γ/δ+ T cells were isolated, cultured for 48 h, and supernatant was collected. Placental samples were digested, and trophoblast cells were cultured for 24 h. Trophoblast cells were cultured with 50 …

Comparison Of Gray Squirrel (Sciurus Carolinensis) And Fox Squirrel (Sciurus Niger) In Order To Explore Genes That Confer A Fitness Advantage, Joshua Franzen

UNO Student Research and Creative Activity Fair

Mutations in the MC1R gene is associated with melanism, or black fur, and the presence of a 24 base pair deletion in the MC1R gene of fox and gray squirrels suggests a shared ancestry between the two species. This could be due to a mutation in a common ancestor or previous mating between species. Evidence has shown that the most likely cause for the presence of MC1R in both species is previous mating between species. If this did occur, then it is possible that other genes traveled between species along with the mutated MC1R gene. Conserved genes shared between species …

Impacts Of Digestawell Nrg Supplementation On Post Exercise Muscle Soreness In Unconditioned Horses, A Pilot Study, Jessica K. Suagee-Bedore, Yeting Shen, Shea Porr, Ivan D. Girard, Karen Bennett-Winbrush, Ashley L. Wagner

Faculty & Staff Research and Creative Activity

Exercising horses are commonly plagued by muscle fatigue and soreness, which can result in reduced performance ability. In the present study, ten unconditioned horses were fed 200g per day DigestaWell NRG, a commercial dietary supplement containing Yucca schidigera and Trigonella foenum-graecum, two herbs shown in other species to reduce post-exercise muscle pain and soreness. A control, unsupplemented group contained ten horses of similar age, breed, and gender. Horses completed a 50 minutes, ridden standardized exercise test of moderate intensity immediately prior to (Period1) and after 28 days of supplementation (Period2). Muscle soreness and tightness were evaluated 24 hours prior …

Agriculture Is Adapting To Phenological Shifts Caused By Climate Change, But Grassland Songbirds Are Not, Maeve M. Mcgowan, Noah G. Perlut, Allan M. Strong

Environmental Studies Faculty Publications

Migratory birds time their migration based on cues that signal resource availability for reproduction. However, with climate change, the timing of seasonal events may shift, potentially inhibiting the ability of some species to use them as accurate cues for migration. We studied the relationship between phenological shifts and reproduction by long- and short-distance migratory songbirds—Bobolinks (Dolichonyx oryzivorus) and Savannah Sparrows (Passerculus sandwichensis). Our study population breeds in hayfields and pastures in Vermont, USA, where farmers are also changing management activities in response to climate change. From 2002 to 2019, we monitored nest initiation dates to quantify …

Non-Breeding Ecology Of Loggerhead Shrikes In An Intensive Agricultural Region, Emily Rose Donahue

Student Theses and Dissertations

As intensive agriculture has transformed much of the landscape across central North America, many grassland species have had to learn to adjust these new, highly homogeneous habitats while facing steep population declines. The Loggerhead Shrike (Lanius ludovicianus; hereinafter shrike) is one such declining species that persists in these agricultural landscapes. However, we lack information on the sustainability of these populations, and shrike non-breeding ecology, as for most passerines, remains understudied. Specifically, little is known about non-breeding behavior, diet, survival, site fidelity, and habitat selection. If survival, space use, and habitat selection metrics differ between demographic groups within non-breeding populations also …

Ecological Physiology Of Rafinesque’S Big-Eared Bats (Corynorhinus Rafinesquii) Roosting In An Anthropogenic Structure In Arkansas, Benjamin N. Spitz

Student Theses and Dissertations

Rafinesque’s big-eared bats (Corynorhinus rafinesquii) are suffering habitat loss due to deforestation of bottomland hardwoods which an increase in agriculture forces maternity colonies to roost in anthropogenic structures. In Arkansas I explore how bats from maternity colonies in anthropogenic structures use torpor and how torpor is affected by chronic stress and ectoparasites. During June- August of 2018-2019, I surveyed a maternity colony inside a barn. Captured bats were tagged with temperature-sensitive transmitters and tracked to roost trees and the barn daily confirming day roosts. Using Lotek data loggers, data from transmitters were collected as skin temperature and compared to ambient …

Checklist Of The Inland Fishes Of Louisiana, Michael H. Doosey, Henry L. Bart Jr., Kyle R. Piller

Southeastern Fishes Council Proceedings

Since the publication of Freshwater Fishes of Louisiana (Douglas, 1974) and a revised checklist (Douglas and Jordan, 2002), much has changed regarding knowledge of inland fishes in the state. An updated reference on Louisiana’s inland and coastal fishes is long overdue. Inland waters of Louisiana are home to at least 224 species (165 primarily freshwater, 28 primarily marine, and 31 euryhaline or diadromous) in 45 families. This checklist is based on a compilation of fish collections records in Louisiana from 19 data providers in the Fishnet2 network (www.fishnet2.net). The checklist has grown because of descriptions of three new species, new …

Nutraceutical Intervention With Colostrum Replacer: Can We Reduce Disease Hazard, Ameliorate Disease Severity, And Improve Performance In Preweaned Dairy Calves?, Melissa C. Cantor, D. L. Renaud, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

The objective of this randomized clinical trial was to determine if an intervention with colostrum replacer (CR; Premolac Plus, Zinpro; 125 g/d fed for 3 d) or a placebo milk replacer (Cows Match, Land O'Lakes Inc.; 125 g/d for 3 d) following a triggered alarm could ameliorate disease bouts in dairy calves. The alarm was set to detect negative deviations of milk intake (20% reduction) or drinking speed (30% reduction) in relation to a calf's 12-d rolling average feeding behavior. Calves were enrolled on this study (n = 42 CR, n = 42 placebo) when they triggered an alarm from …

Undergraduate Session Ii: Presentation 3 - Conservation In Zoos: The Effect It Has On Wildlife, Jessica Dawn Alley

Life of the Scholar Multidisciplinary Conference

My presentation will be a powerpoint slideshow explaining background information leading into the research I completed. The research was interviews with conservationists at selected zoos across the country as well as one conservationist that is international.

Cytokine And Haptoglobin Profiles From Shipping Through Sickness And Recovery In Metaphylaxis- Or Un-Treated Cattle, Carol G. Chitko-Mckown, Gary L. Bennett, Larry A. Kuehn, Keith D. Dedonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Aspen M. Workman, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the …

Holocene Records Of Nebraska Mammals, Hugh H. Genoways

Zea E-Books Collection

A survey of the archeological and paleontological literature allowed a compilation of Holocene records of mammals in Nebraska. This survey identified Holocene records from 338 sites in 62 of the 93 Nebraska counties. These counties were located throughout state, but there was a concentration of sites in southwestern Nebraska where there were 27 fossil sites in Frontier County and 22 in Harlan County. Fossils sites were underrepresented in the Sand Hills region. Records of fossil mammals covered the entire Holocene period from 13,000 years ago until AD 1850. A minimum of 57 species (with eight additional species potentially present) representing …

Effects Of Microtubule Stabilizing And Recovery Agents On Vitrified Bovine Oocytes, Emily Dzekunskas

LSU Master's Theses

Oocyte vitrification, while beneficial for research and species conservation, has limited success due to cryoinjury to the meiotic spindle. This study aimed to evaluate treatments to improve meiotic spindle recovery after vitrification by treating bovine oocytes with either a microtubule stabilizing agent, or a microtubule recovery agent. In the first two experiments, Taxol and epothilone B were used to treat bovine oocytes before vitrification. Both compounds have microtubule stabilizing properties and are known antimitotic compounds commonly used in cancer treatment. Oocytes were incubated with one of the microtubule stabilizing agents for 15 minutes before vitrification at one of three concentrations …