Evaluating The Chemical Reactivity Of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding Through Kendrick Mass Defect Analysis, Hannah M. Hamontree, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The emerging risks to organisms of pyrogenic-derived dissolved organic matter (PyDOM) from forest fires are of concern due to its toxic and mutagenic potential (e.g., pro-oxidative responses in fauna through the depletion of glutathione, a nitrogen- and sulfur-containing tripeptide found in cells). This study simulates this phenomenon in a laboratory setting by identifying bonding between reduced l-glutathione and organic molecules in leachates from environmentally weathered biomass samples (charred and uncharred) using Kendrick Mass Defect (KMD) analysis from formula lists obtained from negative-mode electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry ((-)ESI-FT-ICR-MS). These formula lists reveal a 10-fold increase in nitrogen- and sulfur-containing …

Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala

Biological Sciences Faculty Publications

Climate change is expected to intensify salinity and temperature fluctuations in estuarine and coastal habitats. However, the impact of salinity variation on ectotherms’ thermal tolerance remains underexplored, limiting our understanding of the interplay between these stressors. This study investigated salinity-dependent changes in life history traits (survival, growth rate, fecundity, and egg size) over a 33-day exposure to a salinity gradient (12, 17, 22, 27, 32, 35, 40, 45, 50) in the marine annelid Ophryotrocha labronica. Individuals that survived the salinity treatments were assessed for their upper and lower thermal tolerance limits. Female individuals, the target of the study, exhibited …

Cellular Responses Of Astrangia Poculata (Ellis And Solander, 1786) And Its Symbiont To Experimental Heat Stress, Tyler E. Harman, Daniel Barshis, Briana Hauff Salas, Kevin B. Strychar

Biological Sciences Faculty Publications

Climate change has had devastating effects on tropical coral reefs; however, much less is known regarding how heat stress affects temperate coral. This research focuses on Astrangia poculata (Ellis and Solander, 1786) collected from Narragansett Bay, RI, during the summer and winter seasons and understanding the effect of experimental thermal extremes (i.e., 26 °C) on seasonally different populations. Photosynthetic efficiency (Fv/Fm), symbiont density (via an inverse relationship with pixel intensity), and oxidative stress via reactive oxygen species (ROS) concentrations were measured on symbiotic and aposymbiotic A. poculata. Higher Fv/Fm rates were observed in summer- vs. winter-collected corals (p ≤ …

Location Intelligence Unveils Seasonal Spatiotemporal Pattern Shifts Of Habitat Use And Selection By Cattle Across A South Texas Coastal Landscape Infested With Rhipicephalus Microplus, Taylor G. Donaldson, Brian T. Rich, Miranda R. Peterson, Hsiao-Hsuan Wang, William E. Grant, Donald B. Thomas, Kimberly H. Lohmeyer, Adalberto Á. Pérez De León, Pete D. Teel

Biological Sciences Faculty Publications

Knowledge gaps on the dynamics of cattle fever tick-cattle-habitat-climate interactions in South Texas and their influence on the efficacy of treatments to eliminate infestations with the Rhipicephalus (Boophilus) microplus prevent optimal interventions by the Cattle Fever Tick Eradication Program (CFTEP). The CFTEP has been operating in the USA since 1907. This study applied the concept of location intelligence to examine movement, habitat use and selection by cattle in a highly heterogeneous coastal landscape infested with R. microplus. Cattle interface with white-tailed deer and nilgai, which are alternate wildlife hosts of R. microplus, in this unique South …

Selective Nutrient Incorporation May Underestimate Heterotrophy Of A Mixotrophic Reef-Building Coral, Connor R. Love, Marleen Stuhr, Michael D. Fox, Veronica Z. Radice, Maoz Fine, Kelton W. Mcmahon, David L. Valentine

Biological Sciences Faculty Publications

Mixotrophic reef-building corals acquire nutrition via photosynthate translocation from endosymbiotic microalgae and by heterotrophic prey/particle capture. Heterotrophy promotes resistance to, and recovery from, environmental stress, but quantifying coral heterotrophy remains difficult due to complex resource exchanges within the coral holobiont. We interrogated the response of multiple biomarkers to coral heterotrophy using fatty acid profiling and stable isotope analysis of Stylophora pistillata grown along a controlled feeding gradient from pure autotrophy to pure heterotrophy. We found that fatty acids and nitrogen were effectively incorporated into both coral host and symbiont tissues, while carbon, which is the primary target for conventional heterotrophy …

Biotime 2.0: Expanding And Improving A Database Of Biodiversity Time Series, Maria Dornelas, Laura H. Antão, Amanda E. Bates, Viviana Brambilla, Jonathan M. Chase, Cher F. Y. Chow, Ada Fontrodona-Eslava, Anne E. Magurran, Inês S. Martins, Faye Moyes, Alban Sagouis, Samuel Adu-Acheampong, Daniel Acquah-Lamptey, Dušan Adam, Penelope A. Ajani, Aitor Albaina, Pablo Almaraz, Jeongseop An, Roger Sigismund Anderson, Madelaine Jean Robertson Anderson, Alexsander Z. Antunes, Ivan Arismendi, Linda Armbrecht, Pedro Aros-Mardones, Sreejith Kalpuzha Ashtamoorthy, Narayanan Ayyappan, Gal Badihi, Joseph J. Bailey, Andrew H. Baird, Mark Edward Baird, Sreekumar Vadakkethil Balakrishnan, José António L. Barão-Nóbrega, Adi Barash, Miguel Barbosa, Jos Barlow, Claus Bässler, Matthieu Beaumont, Natalie Beenaerts, Tiago Octavio Begot, Wallace Beiroz, Ricardo Beldade, David M. Bell, Alecia Bellgrove, Jonathan Belmaker, Lisandro Benedetti-Cecchi, Cassandra E. Benkwitt, Pamela Medina-Van Berkum, Brandon T. Bestelmeyer, Matthew C. Betts, Maxwell Kelvin Billah, Anne D. Bjorkman, Magdalena Błażewicz, Christopher P. Bloch, Shane A. Blowes, Antonio Bode, Juliano A. Bogoni, Thomas Bolger, Timothy C. Bonebrake, Erik Bonsdorff, Roberta Bottarin, Luke N. Brokensha, Rob W. Brooker, Andrew J. Brooks, Helge Bruelheide, Thiago Almeida Bueno, Claire Laguionie, Mariana Lopes Campagnoli, James Cant, Erica Pellegrini Caramaschi, Alexandre Caron, Tadhg Carroll, Tancredi Caruso, Juan Carvajal-Quintero, Giuseppe Castaldelli, Edward Castañeda-Moya, Pedro V. Castilho, Sonia Zanini Cechin, Shahar Chaikin, Uchangi Manjunatha Chandrashekara, Tory J. Chase, Chaolun Allen Chen, Jorge José Cherem, Sei-Woong Choi, Erica M. Christensen, Alexander V. Christianini, Jackson Wing Four Chu, Peter Coad, Carl Van Colen, Lise Comte, Elisabeth J. Cooper, J. Hans C. Cornelissen, Eddy Cosson, Unai Cotano, Luc Crevecoeur, Shannan Kyle Crow, Graeme S. Cumming, Vanessa S. Daga, Gabriella Damasceno, Gergana N. Daskalova, Claire H. Davies, Robert A. Davis, Frank P. Day, Sussy De-La-Zerda, Amy Elizabeth Deacon, Indradatta De Castro-Arrazola, Steven Degraer, Kharran Deonarinesingh, Juan C. Diaz-Ricaurte, Christopher R. Dickman, Tara Dirilgen, Ciaran John Dolan, J. Emmett Duffy, Timothy E. Dunn, Giselda Durigan, Ciara Dwyer, Steven Earl, Dor Edelist, Graham John Edgar, Sally Edmonson, Ashley K. Elgin, Kari Elsa Ellingsen, Sarah C. Elmendorf, Ruth S. Eriksen, S. K. Morgan Ernest, Ruben Escribano, Paula Cabral Eterovick, Brian S. Evans, Jason D. Everett, Vesela Evtimova, Dan A. Exton, Andrew J. Fairbairn, Filipe Moreli Fantacini, Fabiano Turini Farah, Fábio Zanella Farneda, Mario E. Favila, Philippe Fernandez-Fournier, Braulio Fernández-Zapata, Diogo F. Ferreira, Carola Ferronato, Christopher R. Du Feu, Alessandra Fidelis, David A. Fifield, Vilmar Picinatto Filho, Walter Mesquita Filho, Robert N. L. Fitt, Carlos A. H. Flechtmann, William R. Fraser, Donna L. Fraser, Lídia Freixas, John Fryxell, Garrett J. Fundakowski, Scott Stanley Gabara, Elise Gallois, Mariana Garcia Criado, Emili García-Berthou, Joaquim Garrabou, Andrew R. Gates, Roberto Cazzola Gatti, Anna Gavioli, Tal Gavriel, Benoit Gendreau-Berthiaume, Xingli Giam, Carina Gjerdrum, Michael Glemnitz, Jasmin Annica Godbold, Daniel Gómez-Gras, Rodrigo Barbosa Gonçalves, Andy Goold, Richard R. Gordon, Menachem Goren, Fernando Vilas Boas Goulart, William G. Gould, Meagan M. Graboski, Nicholas A. J. Graham, Maurício Eduardo Graipel, Laura J. Grange, Aaron C. Greenville, Gary D. Grossman, Valeria A. Guinder, Peter Haase, Gary N. Haskins, Kris Havstad, Luise Hermanutz, Michael Julian Hames Hickford, Pamela Hidalgo, Pedro Higuchi, Andrew S. Hoey, Gert Van Hoey, Annika Hofgaard, Kristen T. Holeck, Robert D. Hollister, Richard T. Holmes, Mia Odell Hoogenboom, Joaquín Hortal, Tammy Horton, Chih-Hao Hsieh, Christine L. Huffard, Ida-Maria Huikkonen, Allen H. Hurlbert, Julian Hynes, Pascal Irz, Natalia Macedo Ivanauskas, Akemi Iwayama, Darren K. James, Ute Jandt, Anna M. Jażdżewska, Merlijn Jocque, Sophie T. Johnston, Samuel E. I. Jones, Faith A. M. Jones, Julia A. Jones, Edite Jucevica, Ugis Kagainis, Maiko Kagami, Jungwon Kang, Xuejia Ke, Erin Colleen Keeley, Rebecca Kinnear, Kari Klanderud, Uwe Klinck, Roel Van Klink, Stefan Klotz, Carolien Kockaert, Halvor Knutsen, Matti Koivula, Alessandra Kortz, Peter Kriegel, Chao-Yang Kuo, David J. Kushner, Rosina Kyerematen, Raphaël Lagarde, Lesley T. Lancaster, Ori Frid Landau, Wouter Van Landuyt, Eric R. Larson, Mai Lazarus, Cheol Min Lee, Jonathan S. Lefcheck, Jonas J. Lembrechts, Renato A. Ferreira De Lima, Romullo Guimarães Lima, Nathália G. S. Lima, Cristina Linares, Sandra C. Lindstrom, Francisco Lloret, John David Lloyd, Cleonice Maria Cardoso Lobato, David M. Lodge, Peter Richard Long, Celeste López-Abbate, Adrià López-Baucells, Julio Louzada, Maite Louzao, Antonella Lugliè, Micheli Ribeiro Luiz, S. Ellen Macdonald, Joshua S. Madin, André Lincoln Barroso Magalhães, Rajindra Mahabir, David Maphisa, Thomas Edward Martin, Marcio Martins, Patrick T. Martone, Silvia Matesanz, Shin-Ichiro S. Matsuzaki, Thomas J. Matthews, Iain Mccombe Matthews, Connie J. Maxwell, Kent P. Mcfarland, Brian J. Mcgill, Diane Marie Mcknight, Michael J. Mcwilliam, Jason Meador, Henning Meesenburg, Kristin Meier, Viesturs Melecis, Peter L. Meserve, Christoph F. J. Meyer, Anders Michelsen, Natali Olivia Roman Miiller, Marco Milardi, Nataliya Milchakova, Robert J. Miller, Jonathan Millett, Tom Moens, Luciano F. A. Montag, Jon Moore, Jörg Müller, Akhil Murali, Shauna Ann Murray, Isla H. Myers-Smith, Randall W. Myster, Masahiro Nakamura, Sasi Nayar, Francis Neat, James A. Nelson, Michael Paul Nelson, Boris P. Nikolov, Rym Nouioua, Collins Ayine Nsor, Michael O' Connor, Edward Adzesiwor Obodai, Amy Marie Offland, Romà Ogaya, Hisako Ogura, Thomas A. Okey, Julian D. Olden, Luiz Gustavo Rodrigues Oliveira-Santos, Jeffrey C. Oliver, Esben Moland Olsen, Vladimir G. Onipchenko, Daniel Oro, Dais Ozolins, Krzysztof Pabis, Bachisio Mario Padedda, Facundo X. Palacio, Alain Paquette, Sinta Trilestari Pardede, David M. Patersib, Sarah Pausina, Raphaël Pélissier, Steven C. Pennings, Josep Penuelas, Felipe Walter Pereira, Nivaldo Peroni, Sergio Picó, Francesca Pilotto, Hudson Tercio Pinheiro, Oscar Pizarro, Roberto Pizzolotto, Francesco Pomati, Paulo Santos Pompeu, Dominique Ponton, Eric Post, Nicolas Poulet, Juha Pöyry, Steven J. Presley, Herbert H. T. Prins, Pieter Provoost, Kathleen L. Prudic, Vignesh Punjayil, Petr Pyšek, Pascal Querner, Juan Pablo Quimbayo, Indar W. Ramnarine, Daniel C. Reed, Peter Bernard Reich, Suzanne M. Remillard, Cerren Richards, Anthony James Richardson, Itai Van Rijin, Victor H. Rivera-Monroy, Christian Rixen, Kevin Peter Robinson, Ricardo Rocha, Ricardo R. Rodrigues, Cassy Rodrigues, Bjørn De Roos, Denise De C. De Rossa-Feres, Loreta Rosselli, Peter Charles Rothlisberg, Ana Rubio, Lars G. Rudstam, Catalina S. Ruz, Nancy B. Rybicki, Gunther Van Ryckegem, Andrew L. Rypel, Jon P. Sadler, Victor Satoru Saito, Sofia Sal, Renato Portela Salomão, Nathan J. Sanders, Flavio A. M. Santos, Tiago Gomes Dos Santos, Swapan Kumar Sarker, Sara E. Scanga, Marcus Schaub, Jochen Schmidt, Inger Kappel Schmidt, Robert L. Schooley, Alfred Schultz, Alberto Scotti, Amanda Serpell-Stevens, Filipe C. Serrano, Elizabeth H. Shadwick, Matthew Shaft, Thomas W. Sherry, Erika Mayumi Shimabukuro, Jacek Siciński, Caya Sievers, Fernando Rodrigues Da Silva, Ana Carolina Da Silva, Juliana M. Silveira, Tadeu Siqueira, Arunkumar Kavidapadinjattathil Sivadasan, Prasad Theruvil Parambil Sivan, Agnija Skuja, Amalia L. Slaughter, Jasper A. Slingsby, Joseph R. Smith, Bruno Eleres Soares, Martin Solan, Flaviana Maluf Souza, Gabriel B. G. Souza, Joshua L. Sprague, Ulrich Stachow, J. John Stadt, Christopher D. Stallings, Radoslav Hristov Stanchev, Emily H. Stanley, Brian M. Starzomski, Jose Mauro Sterza, Maarten Stevens, F. Gary Stiles, Stefan Stoll, Rick D. Stuart-Smith, Yzel Rondon Súarez, Laura Super, Sarah R. Supp, Tapio Sutela, Iain M. Suthers, Anna Suuronen, Kerrie M. Swadling, Daniel K. Szydlowski, Hisatomo Taki, Sara Jeanne Snell Taylor, Pablo A. Tedesco, Nils Teichert, Akira Terui, Gary P. Thiede, Anne Thimonier, Oliver Thomas, Peter Allan Thompson, Simon Thorn, Jeremy S. Tiemann, Luís Felipe Toledo, Anne Tolvanen, Maria Teresa Zugliani Toniato, Ignasi Torre, Marcos Adriano Tortato, Kumiko Totsu, Andrew Trant, Robert R. Twilley, Hirokazu Urabe, Pierre Valade, Nelson Valdivia, Martha Isabel Vallejo, Thomas J. Valone, Jan Vanaverbeke, Tiago Silveira Vasconcelos, Teppo Vehanen, Fábio Venturoli, Hans M. Verheye, Hendrik Jannes Wietse Vermeulen, Arne Verstraeten, Marcelo Vianna, Rui Vieira, João Paulo Santos Vieira-Alencar, Marc Vilella, Jean Ricardo Simões Vitule, Lien Van Vu, Robert B. Waide, Paige S. Warren, Joseph Paul Wayman, Sara L. Webb, Benjamin Weigel, Ellen A. R. Welti, Fritha West, Fulgor Westermann, Matthew A. Whalen, Ethan P. White, Claire E. Widdicombe, Richard Williams, Mark Williamson, Michael R. Willig, Sonja Wipf, Eric J. Woehler, Alje Woldering, Kerry D. Woods, Wu-Bing Xu, Ruthy Yahel, Zeren Yang, Kyle J. A. Zawada, Camila Zornosa-Torres, Assaf Zvuloni

Biological Sciences Faculty Publications

Motivation: Here, we make available a second version of the BioTIME database, which compiles records of abundance estimates for species in sample events of ecological assemblages through time. The updated version expands version 1.0 of the database by doubling the number of studies and includes substantial additional curation to the taxonomic accuracy of the records, as well as the metadata. Moreover, we now provide an R package (BioTIMEr) to facilitate use of the database.

Main Types of Variables Included: The database is composed of one main data table containing the abundance records and 11 metadata tables. The data are organised …

Précis Of The Edge Of Sentience: Risk And Precaution In Humans, Other Animals, And Ai, Jonathan Birch

Animal Sentience

We often face grave practical decisions that seem to hinge on whether a system is sentient. This family of cases includes invertebrate animals, people who are unresponsive after brain injury, fetuses, neural organoids, and now AI technologies. We must decide what to do despite ongoing disagreement about the nature of sentience. In our state of uncertainty, we should pragmatically transform the question from “Is it sentient?” to “Is it a sentience candidate, an investigation priority, or neither?”. When a system is a sentience candidate, it is negligent to fail to consider precautions. We should instead evaluate precautions for their proportionality …

A Multispecies, Multisite Assessment Of Coral Thermal Tolerances In American Samoa, Using Acute Heat Stress Assays, Ponchanok Weeriyanun, Nicolas R. Evensen, Katherine E. Parker, Veronica Z. Radice, Daniel J. Barshis

Biological Sciences Faculty Publications

Reef-building corals are being impacted by increasing seawater temperatures, with marine heatwaves leading to mass bleaching events and extensive mortality. However, corals can display variation in thermal tolerance, both within and among species. In this study, we sampled four coral species (Acropora hyacinthus, Montipora grisea, Pocillopora verrucosa, and Porites lobata) from two sites on Tutuila, American Samoa, with contrasting environmental regimes but similar Maximum Monthly Means (MMM). Coral fragments were subjected to short-term acute heat stress experiments using the Coral Bleaching Automated Stress System (CBASS). PAM Fluorometry was used to measure the symbiont’s effective quantum yield (Fv/Fm) following heat stress …

Herpetofauna Richness, Diversity, And Occurrence At The Northern Ecotone Of Longleaf Pine, Julianne Jones, Dylan Bryant, Erik Yando

Biological Sciences Faculty Publications

Habitat suitability commonly differs between the core and periphery of ecosystems. The longleaf pine ecosystem is recognized for its value in providing habitat to a variety of specialist and endemic species, especially herpetofauna. However, at the northernmost extent of longleaf pine (in southeastern Virginia) little is known about the denizen reptiles and amphibians. Moreover, the remaining longleaf pine ecosystem at the latitudinal ecotone is fragmented amongst a mosaic of other ecosystems. We examined herpetofauna diversity, richness, and capture rates between four focal habitat types—mature longleaf pine, restored longleaf pine, maple-gum swamp, and pocosin-bog—in the Zuni Pine Barrens of southeastern Virginia. …

Assemblage Structure Of Large Carnivorous Coral Reef Fishes At Shallow And Mesophotic Depths In Two Of The Largest And Most Isolated Marine Protected Areas In The Philippines, Rene A. Abesamis, Mikaela L. Salvador, Ryan Murray, Kymry Delijero, Segundo F. Conales, Jean T. Utzurrum, Christopher E. Bird, David T. Gauthier

Biological Sciences Faculty Publications

Overfishing remains a threat to coral reef fishes globally, with large carnivores often the most vulnerable. Marine protected areas (MPAs) can restore fish populations and biodiversity, but their impact has been understudied in mesophotic coral ecosystems (MCEs – extensions of shallow reefs from 30 to 150 m). We analyzed baited remote underwater videos recorded in 2016 to investigate the assemblage structure of large carnivorous fishes at shallow (4–12 m) and mesophotic (45–96 m) depths in two of the largest and most isolated MPAs in the Philippines: Tubbataha Reefs Natural Park (TRNP), a fully no-take MPA enacted in 1988; and Cagayancillo, …

Validating A Novel Capability Of Assessing Pathways Of Animal Water Gain And Loss, Zachary T. Steele, Karen Caceres, Zachary A. David, Lisa M. Shollenberger, Alexander R. Gerson, Seth D. Newsome, John P. Whiteman

Biological Sciences Faculty Publications

Understanding variations in the routes by which wild animals gain and lose water is challenging, and common methods require longitudinal sampling, which can be prohibitive. However, a new approach uses Δ′¹⁷O_BW (Δ′¹⁷O of animal body water), calculated from measurements of δ′¹⁷O and δ′¹⁸O in a single sample, as a natural tracer of water flux. Δ′¹⁷O_BW is promising, but its relationship to organismal variables such as metabolic rate and water intake have not been validated. Here, we continuously measured oxygen influxes and effluxes of captive deer mice (Peromyscus maniculatus), and manipulated their water intake and metabolic rate. We used …

Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre

Biological Sciences Faculty Publications

The feeding ecology of wildlife populations has important implications for individual health, population productivity and distribution patterns. For ursids (bears), food resources and feeding behaviour primarily affect population dynamics via effects on cub production and survival. Much of what is known about the feeding ecology of bears is based on analyses of tissues collected from capture-based research efforts, harvested animals or non-invasive approaches. However, inference about diet from hair has been limited by a lack of quantitative data on the timing of the moult and hair growth rates. We conducted a study to develop and test two methods of quantifying …

Food Finding Ability Does Not Predict Annual Survival In Winter Resident Birds, Aidan Hand

Honors Program: Senior Projects (Public)

Small non-migratory birds in temperate locations face many challenges to survive the winter. They must be able to find enough food, which is in decreased supply, to fuel the energetic demands of staying warm. Prior work has demonstrated that birds show individual variation in their foraging behaviors that could contribute to the discovery of new foraging patches, such as novel environment exploration. A better ability to discover new food sources could increase a bird’s chance of surviving the winter by increasing its access to new food patches. To test this hypothesis, we used radio frequency identification (RFID)-enabled bird feeders to …

Karyotyping As A Method To Verify Phylogenetic Relationships Among The Enneacanthus Species, Ophelia A. Anderson

Theses and Dissertations

The Enneacanthus genus consists of three species of freshwater fish endemic to eastern North America: E. obesus (Banded Sunfish), E. gloriosus (Blue Spotted Sunfish), and E. chaetodon (Black Banded Sunfish). Previous studies using molecular data suggest that E. obesus and E. gloriosus are more closely related, with E. chaetodon as the most distantly related species in the genus. This study investigates the phylogenetic relationship among the Enneacanthus species, using karyotype characteristics - chromosome number, morphology and fundamental number - as comparative metrics. Chromosome spreads were obtained from scale and gill epithelium samples collected between 1993 and 1994. Prepared slides …

Behavioral Observations And Analysis Of Twix: The Cape Porcupine At The Lake Superior Zoo, Erin Davidson

Scripps Senior Theses

The three main goals of zoos are outlined as: education, conservation, and research. In our modern world, the focus of zoos has shifted away from simply housing animals, and towards how we can best promote proper animal welfare for individuals in human care. Animal welfare can be defined as the “mental health” of non-human species of fauna, and is greatly influenced by the stressors within the environment, as well as the option of choosing how to interact with and respond to the environment. Animals housed in zoos are presented with unique stressors that they often do not choose to experience, …

Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill

Williams Honors College, Honors Research Projects

With the use of zebrafish as a common model organism for human health, the reduction of confounding variables in their care is key. Many commercialized zebrafish diets are available for purchase, but each one consists of a different ratio of these crucial macronutrients. This study aimed to determine the effects long-term diets high in one macronutrient would have on the morphometrics, heart, liver, and protein content of zebrafish. Four diets were fed over a period of almost two years- high lipid, high carb, high protein, and a control diet. The control diet was the standard feed used in this lab, …

Exploring The Effects Of Road Salt On Earthworm Species, Alea Mry Koston

Williams Honors College, Honors Research Projects

This study examines the effects of two road salts, sodium chloride (NaCl) and calcium magnesium acetate (CMA), on the health of two earthworm species: Eisenia fetida and Lumbricus terrestris. Over a 4-week period, approximately 168 earthworms were exposed to varying concentrations of either NaCl, CMA, or mixed ratios of both, with a control group included for comparison. The experiment involved monitoring the changes in earthworm weight and measuring their contraction rates to determine physiological responses. Statistical analysis revealed no significant differences in weight or activity between NaCl and CMA treatments for either species. However, higher concentrations of NaCl resulted in …

Elongate Locomotors Show Consistent Torque Patterns Across Substrates, Spencer Bradford, Henry Astley

Williams Honors College, Honors Research Projects

The four main movements found in snakes are understood mainly due to external study; however, muscular forces and resulting torques are poorly known. The main research objective is to study the torque production on different substrates using a snakebot. The study will give a rudimentary model for torque production on different substrates. It can then be compared against biological systems to understand snake movement at a muscular level. This study used a snakebot, allowing direct assessment of the current being run through each of its motors (proportional to torque). Data was then examined using a Principal Component Analysis test for …

Development Of A Proposed Selective Medium For The Isolation And Differentiation Of P. Sordellii, Dimyana Bottros

Honors Undergraduate Theses

Clostridium, a genus of anaerobic, spore-forming bacteria, includes clinically and ecologically significant species like Clostridium difficile and Paeniclostridium sordellii. C. difficile causes antibiotic-associated colitis, while P. sordellii is linked to fatal infections and spontaneous abortions. Recent studies found both species in marine sediments, suggesting their role in environmental and wildlife health disturbances, including the 2013 mass mortality of over 300 manatees in the Indian River Lagoon. However, isolating and differentiating P. sordellii is challenging due to the lack of selective media optimized for its growth. This thesis proposes a novel selective medium tailored to P. sordellii’s physiological …

Development Of A Stable Isotope Method To Measure Protein Fractional Synthesis Rate In Equine Skeletal Muscle, Antoinette E. Foliart

Theses and Dissertations--Animal and Food Sciences

Muscle mass is regulated by the balance between muscle protein synthesis and degradation. Previous research in horses has shown that the signaling pathway that regulates protein synthesis is upregulated with increasing protein intake; however, whether this corresponds to an increase in muscle protein synthesis rates had not been previously explored. The objective of this study was to develop an isotopic method to measure muscle protein fractional synthesis rate (FSR) in horses following graded protein intake. Eight mature Thoroughbred horses were studied using a randomized cross-over design. Horses consumed meals containing 0 (No), 0.125 (Low), 0.25 (Med), or 0.5 (High) g …

Lives And Lessons Of Zoo Animals, Nicole Emlen

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This project was an interdisciplinary museum exhibit that was displayed at the Montana Natural History Center between April 4 and May 12, 2025. It displays my photographs that I took of some of the animals I worked with during my summer 2024 internship at ZooMontana. In this project, I educate the public about three main functions of zoos--rescue, conservation, and education--through the stories and lives of four species. These animals all have unique stories that are important to share to improve public knowledge of why zoos are important and how we can improve relationships between humans and wildlife. Photography is …

Assessing Intra-Seasonal Changes In Summer Elk Diet Composition, Ava E. Window

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Elk (Cervus canadensis) require adequate forage to fulfill their seasonal nutritional requirements. Elk undergo their highest nutritional demands during summer to support critical life functions such as late gestation, lactation, and juvenile growth. However, variations in plant phenology significantly influence the quality and quantity of forage available to elk during the summer season. As summer advances and plant communities mature, forage quality often declines, leading to a landscape that imposes greater nutritional constraints on elk. As a result, prior research on elk foraging habits has primarily focused on late summer. This has led to limited knowledge surrounding the …

Monograph Available: Urban Coyotes As A Source Of Conflict With Humans: An Evaluation Of Common Management Practices

Human–Wildlife Interactions

This is an announcement about the published monograph titled "Urban Coyotes as a Source of Conflict with Humans: An Evaluation of Common Management Practices." This publication, part of the HWI Monograph Series, is online and open access.

Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger

Electronic Theses and Dissertations

The objective of this study was to determine the influence of vibration during transportation on beef sirloin water holding capacity, color, and tenderness. USDA Choice center-cut sirloin top butts (n = 45 cases) were collected at a commercial processing facility and randomly allocated to one of three treatments 1) regular pallet driven 241 km (Reg241), 2) regular pallet driven 644 km (Reg644), and 3) vibration isolation pallet driven 644 km (Iso644). All subprimals were transported at -2.2 °C (d0) and stored for 10 days to mimic industry practices for product aging. Following storage (d11), one subprimal per case was evaluated …

Fluke Egg Sedimentation Test Procedure, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The department’s approved procedure for detecting trematode eggs and the Eimeria leuckarti sedimentation method (FEST) on faecal samples.

Estimating The Carbon Footprint Of Sheep Production In Western Australia, Tafesse Estifanos, Christophe D'Abbadie, Johnny Machon, Amir Abadi Abadi Ghadim, Gus Manatsa, Paul Mattingley, Ross Kingwell, Kevin Foster

Farm Systems and Economics published reports

Western Australia’s broadacre mixed farming systems combine cropping and sheep production and are the largest component of the state’s agricultural sector. The south west Western Australia (WA) agricultural area hosts about 98% of the state's sheep flock. The sheep industry contributed $1.5 billion to the state’s economy in 2021-22 and accounts for about a quarter of on-farm greenhouse gas (GHG) emissions. Most sheep industry emissions (over 80%) come from methane produced by the sheep. Despite this environmental impact, only 9.9% of Australian sheep producers have estimated their emissions, which limits the industry's ability to monitor and manage carbon footprints effectively. …

Investigating Relatedness Within Breeding Units Of The Highly Social Bushtit (Psaltriparus Minimus), Fiona Skye Connelly

Theses and Dissertations--Biology

Cooperative breeding systems represent an array of varied reproductive strategies and remain a topic of interest in behavioral ecology (i.e., Riehl 2013, Cornwallis et al. 2017). Different manifestations of cooperative breeding, such as cooperative polyandry, no doubt have unique evolutionary pressures tied to local ecologies which have led to their proliferation. Of particular interest are species that show flexibility in their use of reproductive strategies, wherein individuals may choose one strategy over another given a specific circumstance. Here, I used double-digest restriction site-associated DNA sequencing (i.e., ddRAD-seq) to investigate bushtit (Psaltriparus minimus) reproductive strategies and the relatedness between …

The Intermediate And Remote Effects Of Acute Juvenile Stress Upon Auditory Fear Learning, Recognition Memory, And Anxiety Measures In A Rodent Model Of Ptsd, Laura J. Baron

Electronic Theses & Dissertations (2024 - present)

Abstract

Early-life stress is a risk factor for increased susceptibility to stress- and trauma-related disorders, including Posttraumatic Stress Disorder (PTSD). PTSD is characterized by exposure to trauma that results in exaggerated and extinction-resistant fear responses that persist for over a month. Understanding how the severity of early-life stressors in animal models impacts adult behavior, cognition, and underlying neurobiology could aid in the development of more effective PTSD treatments.

Various early-life stress models, especially in infant rats, have been used to induce stress and assess PTSD like-symptoms. Different stressors impact short- and long-term behavior, with some effects influenced by developmental timing …

Using Algal Turf Scrubber Biomass As A Feed Source For Juvenile Eastern Oysters, Taofeek Saka Jimoh

College of Graduate Studies: Theses & Dissertations

Algal turf scrubber (ATS) is an engineered system that promotes natural wastewater treatment processes and improves water quality by passing a shallow stream of nutrient rich water over the surface of a gently sloped flow-way. Algae turf scrubber biomass is a combination of micro and macroalgae, as well as micro-invertebrates, ciliates, bacteria, and more. The overall aim of the study was to reduce the feeding cost of juvenile eastern oysters by replacing the commercial microalgae diet with ATS biomass. The experiment was designed to assess the viability of replacing commercial microalgal diet with ATS biomass at different substitution levels (0, …

Microbial Community Stabilization By Restored Oyster Reefs: Implications For Blue Carbon Storage, Natalie Boydstun

College of Graduate Studies: Theses & Dissertations

Coastal ecosystems provide critical carbon sequestration services, yet the microbial mechanisms underlying carbon storage remain poorly understood, particularly in non-vegetated habitats. This study investigated whether restored oyster reefs (Crassostrea virginica) enhance sediment carbon storage potential by stabilizing microbial communities and promoting efficient organic matter processing. I hypothesized that reef-mediated sediment stabilization and organic deposition would create conditions favoring microbial degradation of complex carbon compounds into stable soil organic matter, ultimately supporting anaerobic carbon storage. To test this, I compared sediment biogeochemistry, enzyme activity, and microbial community structure between three restored oyster reef sites and two unstructured control sites …