Animal Sciences Commons^™

Dissection Of Qtl On Host Chromosome 12 Uncovers Candidate Gene And Missense Polymorphism Associated With Porcine Circovirus 2 Susceptibility, Lianna Rayne Walker

Department of Animal Science: Dissertations, Theses, and Student Research

Porcine circovirus 2 (PCV2) is a small single stranded DNA virus responsible for a group of detrimental diseases referred to as Porcine Circovirus Associated Diseases (PCVAD). Observed variation in incidence and severity of PCVAD between pigs suggests a host genetic role in facilitating PCV2 pathogenesis. This study builds on prior research by Engle et al. (2014), who performed a large-scale genome-wide association study of 974 crossbred pigs experimentally infected with a PCV2b isolate and provided evidence of a host genetic role in PCV2 viremia, immune response, and growth. Two major Quantitative Trait Loci (QTL) were identified for viral load located …

Animal-Assisted Activities Survey At The University Of Nebraska-Lincoln: Assessing Student Opinions And Determining Possible Implementation Strategy, Emma Winters

Honors Program: Senior Projects (Public)

In most situations, animals are not allowed on the University of Nebraska-Lincoln (UNL) campus, including animals used for animal-assisted activities (AAA). Animal-assisted activities have been shown to provide numerous benefits to participants and there could be positive effects to offering AAA at UNL. In order to gauge the opinions of students regarding AAA and how AAA could best be implemented on the UNL campus, a survey was emailed to Animal Science majors and students enrolled in ASCI 271 and ASCI 321 at the University of Nebraska-Lincoln. A request to participate was also included in the weekly College of Agricultural Sciences …

Impact Of Basal Diet On Obesity Phenotype Of Recipient Mice Following Fecal Microbiome Transfer From Obese Or Lean Human Donors. Appendix D. Microbiome Sequencing Data, Daphne Rodriguez, Abby D. Benninghoff, Korry Hintze

Browse all Datasets

The complete data set generated by 16S rRNA sequencing of all fecal samples analyzed is provided as a digital data .biom file accessible.

Zinc Injection As A Novel Castration Method And Carry-Over Effects Of Growth-Promoting Implants In Beef Cattle, Jase Joseph Ball

Graduate Theses and Dissertations

In experiment 1, crossbred bull calves (n = 31; body weight (BW) = 114.3 ± 26.3 kg; age = 119 ± 18.4 d) were allocated to treatments by BW and birthdate. Twenty-seven bull calves were allocated to 3 injectable castration treatments (n = 9 calves/injectable castration treatment) to reflect 3 dosage levels of zinc. Intact bulls had greater (P < 0.001) serum testosterone concentrations compared to bulls injected with zinc. In experiment 2, crossbred beef bulls (n = 180) were blocked by initial BW (337 ± 10.9 kg; 6 blocks) and assigned randomly to 1 of 3 treatments on d 0: 1) INJ; received 1 mL (100 mg Zn) of a Zn solution in each testis, 2) BAN; banded, 3) BUL; intact. Final BW was greater (P < 0.01) for INJ (672 kg) and BUL (686 kg) compared to BAN (611 kg). Serum haptoglobin concentration was greater (P < 0.01) in INJ compared to BUL and BAN on d 1, 3, 5, and 7. Zinc injection resulted in sterilization but not castration in feedlot bulls although it was efficacious in castration of young bulls at branding. In experiment 3, crossbred beef steers (n = 106; BW = 96 ± 3.9 kg; age = 74 ± 2.0 d) were blocked by parity of dam (≤ 2 or > 2 parities), stratified by BW, calf age, calf sire, cow BW and body condition score to 1 of 4 treatments: 1) RALG, Ralgro, Ralgro, Revalor XS, at branding (D 0), weaning (D 156), and feedlot processing (D 325), 2) COMP, Component …

Towards Quantifying Relevant Land Cover Change: A Case Study In The Central Flyway Of The Monarch Butterfly, Hanna Lenee Ford

Graduate Theses and Dissertations

Over the past 20 years notable decreases in monarch butterfly populations have led researchers to begin evaluating the landscape for changes and seeking out opportunities for enacting conservation programs to better support their survival. The monarch butterfly has recently come under consideration for listing under the Endangered Species Act which has created a need for a more informed view of the landscape through which the migrate and breed, the central United States. In this research three spatially-explicit models are explored using the most applicable datasets currently available to address pressing policy and land manager decisions regarding monarch butterfly and pollinator …

Evaluation Of The Efficacy Of A Candidate Turkey Dermatitis/Cellulitis Oil Emulsion Vaccine On Immune Response, Morbidity, And Mortality Under Laboratory And Commercial Conditions, Brittany Danielle Graham

Graduate Theses and Dissertations

Alpha-toxigenic Clostridium septicum (CS), the cause of turkey cellulitis, results in devastating mortality with high costs for the industry. Various vaccinations have been evaluated to prevent this disease with moderate success. Ability of a CS bacterin-toxoid, in conjunction with adjuvants such as aluminum hydroxide, mannoslyated chitosan, or a water-in-oil Seppic Montanide 71 R VG adjuvant (OE) to induce immunity was evaluated in a 7-week study (Experiment 1). Poults (20/group) were vaccinated day-of-hatch, boosted at 5 weeks-of-age and compared to unvaccinated controls. Antibody titers were determined by ELISA for all experiments. In experiment 1, initial vaccination with OE resulted in significantly …

Evaluation Of The Effects Of Formaldehyde On Growth Parameters Of Broiler Chicks, Paula Johnson

Graduate Theses and Dissertations

Formaldehyde has been used as a disinfectant in poultry hatching cabinets to aid in controlling key pathogenic organisms, such as Salmonella and Escherichia coli (E.coli). There is some evidence that prolonged exposure of chicks to formaldehyde can reduce tracheal ciliary function and thus reasons to believe that exposure to formaldehyde, in the absence of pathogen challenge, may reduce chick vitality. It has been found that elevated temperatures during incubation may adversely affect body weights of broiler chicks, as well as post-hatch environmental heat stress on performance in poultry. Post-hatch environmental heat stress has been shown to impact performance in poultry. …

The Effects Of Super-Dosing Phytase On Nursery And Grower Pigs, Joshua Paul Knapp

Graduate Theses and Dissertations

To determine the effects of super-dosing on pigs fed phosphorous and calcium adequate or deficient diets (Experiment 1); the optimum level of corn express phytase (CEP, Experiment 2); and the effects of xylanase/phytase on growth performance (Experiment 3) in pigs fed reduce nutrient diets, weaned pigs were blocked with initial body weight (BW) and allotted to dietary treatments. Treatments were: Positive control (PC), P/Ca adequate diets (NRC 2012); Negative Control (NC), low P (-0.15%) and Ca (-0.12%), +0, 1000, and 4000 FTU/kg of CEP in both PC and NC (Experiment 1); PC, P/Ca adequate diets; NC, decreased P (0.15%) and …

Defending Wild Dogs: Population Dynamics And Disease In Endangered African Wild Dogs, Elizabeth Claire Arredondo

Graduate Theses and Dissertations

African wild dogs (Lycaon pictus) are endangered carnivores whose population is decreasing from habitat loss and fragmentation, interspecific competition, and disease. Survival rates are especially low in Kruger National Park (KNP), though it is unclear why. I estimated the abundance in KNP and survival rates over different time spans, six years and nine months, using public photographic survey data. In 2015, there were 298 (SE=12.1) individuals. Using a mark-recapture analysis in program R, I found that the survival rate between 2009-2015 was only 3.2%, and within the 9-month survey period (September 2014 – June 2015), monthly survival rates for the …

Alternative Feeding Strategies For Growing Cattle Grazing Endophyte-Infected Tall Fescue During The Summer, Kerri A. Johnson

Graduate Theses/Dissertations

Two experiments were conducted to determine animal performance of alternative feeding strategies to heifers grazing endophyte-infected tall fescue during summer months. In Experiment 1, 40 Limousin heifers (261± 40kg initial BW) were stratified by weight and assigned to either a spring harvested tall fescue silage diet or grazing endophyte-infected tall fescue pasture with grain supplement having either natural or artificial shade for 80 days. In Experiment 2, 40 Limousin heifers (277 ± 44kg initial BW) were stratified by weight and assigned to either a traditional grain supplement or feather-meal supplement (rumen bypass arginine supplement) with natural or artificial shade while …

In-Vitro Digestibility And Gas Production Of Wheat Middlings, Solvent Extracted Cottonseed Meal, Soyhulls, And Corn Gluten Feed And The Effects Of Monensin On In-Vitro Digestibility And Gas Production, Dustin W. Devore

Graduate Theses/Dissertations

This study was designed to study the in-vitro digestibility and in-vitro total gas production of corn gluten feed, wheat middlings, solvent extracted cottonseed meal, and soyhulls in multi parous steers. Evaluation of the effects of monensin on in-vitro digestibility and in-vitro total overall gas production of a commercially available complete feed was also studied Feedstuffs were ground through a Wiley mill to ensure uniform samples and were added to digestion jars in a completely randomized design. Rumen fluid was collected from a Bos taurus steer via a ruminal cannula. To ensure consistent digesta material the steer was fed a uniform …

The Relationship Between Measures Of Annual Livestock Disturbance In Western Riparian Areas And Stream Conditions Important To Trout, Salmon, And Char, Lindsey M. Goss, Brett B. Roper

Watershed Sciences Faculty Publications

Managing livestock disturbance in riparian zones in a manner that provides economic returns to ranchers while protecting streams is an important aspect of rangeland management on public lands in the western United States. Attempts to balance economic and ecologic outcomes have been made more difficult due to the presence of several salmonid species that are protected under the Endangered Species Act. One approach to proper management of livestock use near streams has been to define the allowable limits of disturbance using 2 metrics, streambank alteration and stubble height. We evaluated 153 stream reaches within the Interior Columbia Basin to determine …

Ecological Studies Of Wolves On Isle Royale, 2017-2018, Rolf O. Peterson, John A. Vucetich, Sarah R. Hoy

Ecological Studies of Wolves on Isle Royale

Annual Report 2017-2018

Restoration Of Legacy Trees As Roosting Habitat For Myotis Bats In Eastern North American Forests, Michael J. Lacki

Forestry and Natural Resources Faculty Publications

Most eastern North American Myotis roost in forests during summer, with species forming maternity populations, or colonies, in cavities or crevices or beneath the bark of trees. In winter, these bats hibernate in caves and are experiencing overwinter mortalities due to infection from the fungus Pseudogymnoascus destructans, which causes white-nose syndrome (WNS). Population recovery of WNS-affected species is constrained by the ability of survivors to locate habitats suitable for rearing pups in summer. Forests in eastern North America have been severely altered by deforestation, land-use change, fragmentation and inadvertent introduction of exotic insect pests, resulting in shifts in tree …

How Wolves Turned Into Dogs And How Dogs Are Valuable In Meeting Human Social Needs, Kurt Kotrschal

People and Animals: The International Journal of Research and Practice

A wealth of recent behavioral, neurobiological, and genetic results allows us to draw a new, comprehensive picture of the human-wolf- dog relationship. Dogs originated from wolves 35,000 years ago, mainly via selection for tameness. Wolves were probably spiritual partners and hunting buddies of Paleolithic hunter-gatherers over wide areas of Eurasia. Coming together and staying together was probably facilitated by the close ecological and social match between wolves and humans. Both are cursorial hunters and scavengers living in cooperative but relatively closed family groups, which selected for very similar mentalities.

Parallel selection for tameness (i.e., being “nice”) in dogs and humans …

How Frogs Are Hiding In Plain Sight: Phylogenetic Systematics Of Myanmar’S Occidozya Species Complex, Allison Bogisich, Jocelyn Esquivias

Creative Activity and Research Day - CARD

In the frog genus Occidozyga, there is one described species which inhabits Myanmar. However, population estimates for Occidozyga within the country may be inaccurate given unidentified cryptic species. We predict that in Occidozyga, there are more morphologically identical phenotypes- ‘cryptics’- which are actually genetically distinct. Using DNA sequences isolated in the lab at USF from preserved specimens housed at the California Academy of Science, we’ve been able to conduct phylogenetic tree analysis. Preliminary results suggest the presence of two new species within the genus. Phylogeographic analysis of our tree also supports the genetic clustering, such that their evolutionary distribution is …

Time Of Year Influences Social Play In Juvenile Ground Squirrels, Martha Monroy, Madelene Shehan

Creative Activity and Research Day - CARD

Animals must allocate available energy according to priorities that ensure survival and maximize long-term reproductive success. Social play behavior is ubiquitous among juvenile mammals and can have important influences on their social, motor, and cognitive development. We evaluated how the amounts of energy young animals devote to play behavior changes over the course of their annual cycle, as other energetic considerations and constraints change. Young animals may change priorities from play to preparation for hibernation when the annual cycle occurs closer to the usual onset of winter. We used Belding’s ground squirrels (Urocitellus beldingi) as a model system; U. beldingi …

Asas-Ssr Triennnial Reproduction Symposium: Looking Back And Moving Forward—How Reproductive Physiology Has Evolved: Fetal Origins Of Impaired Muscle Growth And Metabolic Dysfunction: Lessons From The Heat-Stressed Pregnant Ewe, Dustin T. Yates, Jessica Lynn Petersen, Ty B. Schmidt, Caitlin N. Cadaret, Taylor L. Barnes, Robert J. Posont, Kristin A. Beede

Department of Animal Science: Faculty Publications

Intrauterine growth restriction (IUGR) is the second leading cause of perinatal mortality and predisposes offspring to metabolic disorders at all stages of life. Muscle-centric fetal adaptations reduce growth and yield metabolic parsimony, beneficial for IUGR fetal survival but detrimental to metabolic health after birth. Epidemiological studies have reported that IUGRborn children experience greater prevalence of insulin resistance and obesity, which progresses to diabetes, hypertension, and other metabolic disorders in adulthood that reduce quality of life. Similar adaptive programming in livestock results in decreased birth weights, reduced and inefficient growth, decreased carcass merit, and substantially greater mortality rates prior to maturation. …

Identification Of Genes Directly Responding To Dlk1 Signaling In Callipyge Sheep, Hui Yu, Jolena N. Waddell, Shihuan Kuang, Ross L. Tellam, Noelle E. Cockett, Christopher A. Bidwell

Animal, Dairy, and Veterinary Science Faculty Publications

Background

In food animal agriculture, there is a need to identify the mechanisms that can improve the efficiency of muscle growth and protein accretion. Callipyge sheep provide excellent machinery since the up-regulation of DLK1 and RTL1 results in extreme postnatal muscle hypertrophy in distinct muscles. The aim of this study is to distinguish the genes that directly respond to DLK1 and RTL1 signaling from the genes that change as the result of muscle specific effects.

Results

The quantitative PCR results indicated that DLK1 expression was significantly increased in hypertrophied muscles but not in non-hypertrophied muscles. However, RTL1 was up-regulated in …

Fetal Demise And Failed Antibody Therapy During Zika Virus Infection Of Pregnant Macaques, Diogo M. Magnani, Thomas F. Rogers, Nicholas J. Maness, Nathan D. Grubaugh, Nathan Beutler, Varian K. Bailey, Lucas Gonzalez-Nieto, Martin J. Gutman, Núria Pedreño-Lopez, Jaclyn M. Kwal, Michael J. Ricciardi, Tereance A. Myers, Justin G. Julander, Rudolf P. Bohm, Margaret H. Gilbert, Faith Schiro, Pyone P. Aye, Robert V. Blair, Mauricio A. Martins, Kathrine P. Falkenstien, Amitinder Kaur, Christine L. Curry, Esper G. Kallas, Ronald C. Desrosiers, Pascal J. Goldschmidt-Clermont, Stephen S. Whitehead, Kristian G. Andersen, Myrna C. Bonaldo, Andrew A. Lackner, Antonito T. Panganiban, Dennis R. Burton, David I. Watkins

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection of pregnant women is associated with pathologic complications of fetal development. Here, we infect pregnant rhesus macaques (Macaca mulatta) with a minimally passaged ZIKV isolate from Rio de Janeiro, where a high rate of fetal development complications was observed. The infection of pregnant macaques with this virus results in maternal viremia, virus crossing into the amniotic fluid (AF), and in utero fetal deaths. We also treated three additional ZIKV-infected pregnant macaques with a cocktail of ZIKV-neutralizing human monoclonal antibodies (nmAbs) at peak viremia. While the nmAbs can be effective in clearing the virus from …

Gene Isoforms Involved In Zebrafish Traumatic Stress Response, Joseph Fischer

Honors Theses

No abstract provided.

Helping Farmers And Reducing Car Crashes: The Surprising Benefits Of Predators, Christopher O'Bryan, Eve Mcdonald-Madden, James Watson, Neil Carter

Human-Environment Systems Research Center Faculty Publications and Presentations

Humans may be Earth’s apex predator, but the fleeting shadow of a vulture or the glimpse of a big cat can cause instinctive fear and disdain. But new evidence suggests that predators and scavengers are much more beneficial to humans than commonly believed, and that their loss may have greater consequences than we have imagined.

Administration Of Eu (+ Fta) And Other Fleets Involved In Aquaculture Of Salmonids, World Maritime University

Research on the Administration of EU (+ FTA) and other Fleets Involved in Aquaculture of Salmonids

t is widely acknowledged that there exists a dearth of information with regard to fleet involved in aquaculture of salmonids. To address this insufficiency, and to understand the gaps and grey areas, Transport Canada funded a project in 2017 that aimed to gain an in-depth understanding of how the Norwegian, Irish, Chile and Australian fleet involved in aquaculture of salmonids are registered and administered. The World Maritime University undertook the project, and contracted external consultants to develop and deliver reports from a number of the aforementioned jurisdictions.

The project report provides a deep insight into the regulatory framework for the …

Effect Of Gestation Length On Litter Size And Piglet Birth Weight, Audrey L. Emery, Dillon Q. Siefert, Courtney K. Moore, Flint W. Harrelson, Patricia L. Harrelson

Celebration of Student Scholarship Poster Sessions Archive

No abstract provided.

Body Size Downgrading Of Mammals Over The Late Quaternary, Felisa A. Smith, Rosemary E. Elliott Smith, S. Kathleen Lyons, Jonathan L. Payne

School of Biological Sciences: Faculty Publications

Since the late Pleistocene, large-bodied mammals have been extirpated from much of Earth. Although all habitable continents once harbored giant mammals, the few remaining species are largely confined to Africa. This decline is coincident with the global expansion of hominins over the late Quaternary. Here, we quantify mammalian extinction selectivity, continental body size distributions, and taxonomic diversity over five time periods spanning the past 125,000 years and stretching approximately 200 years into the future. We demonstrate that size-selective extinction was already under way in the oldest interval and occurred on all continents, within all trophic modes, and across all time …

Comparison Of Equine Behavior To Parelli Horsenality, Rebecca Kirchhoff

Honors Theses

No abstract provided.

Elucidation Of Zebrafish Gene Networks And Pathways Associated With Ptsd, Noor Faisal

Honors Theses

No abstract provided.

A Student Hosted Cattle Sale As An Approach To Experiential Learning, Wade Lang, Courtney Biles

Undergraduate Research Conference

OBJECTIVE: The objective of this study was to determine if a student hosted cattle sale resulted in a beneficial learning experience and if a 30-day reflection period would change student perspective.

Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xvi. Compsobuthus Maidensis Sp. N. (Buthidae) From Somaliland, František Kovařík

Euscorpius

Compsobuthus maidensis sp. n. from Somaliland is described and fully complemented with color photos of specimens, as well as its habitat. Data on the occurrence of the genus Compsobuthus Vachon, 1949 in the Horn of Africa is summarized.

Changes In Myoblast Responsiveness To Tnfα And Il-6 Contribute To Decreased Skeletal Muscle Mass In Intrauterine Growth Restricted Fetal Sheep, Robert J. Posont, Kristin A. Beede, Sean W. Limesand, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth restriction (IUGR) is a leading cause of perinatal morbidity and mortality (Alisi et al., 2011). Skeletal muscle growth is disproportionately reduced in IUGR fetuses and offspring (Padoan et al. 2004; Yates et al. 2014). These individuals present with reduced muscle mass and increased risk for metabolic disorders at all stages of life (Godfrey and Barker, 2000; Yates et al. 2016.). Muscle growth requires proliferation, differentiation, and fusion of myoblasts (muscle stem cells) to form muscle fibers early in gestation and to increase myonuclear content of existing fibers during late gestation and after birth (Yates et al., 2014). These …