Life Sciences Commons^™

101 Ways To Try To Grow Arabidopsis: What Light Intensity Worked Best In This Study? Can High Intensity Discharge Lights Be Used?, Robert Eddy, Daniel T. Hahn, Laura Aschenbeck

Purdue Methods for Arabidopsis Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site [http://www.hort.purdue.edu/hort/facilities/greenhouse/101exp.shtml] regarding best practices for Arabidopsis thaliana growth. Digital images showing treatment differences are included. A summary of recommendations; materials and methods description; and references list are available in separate documents.

Concept, Design And Implementation Of A Cardiovascular Gene-Centric 50 K Snp Array For Large-Scale Genomic Association Studies, Thomas S. Price, Jeffrey C. Barrett, Struan F A Grant, Deborah N. Farlow, Hareesh R. Chandrupatla, Mark Hansen, Saad Ajmal, George J. Papanicolaou, Yiran Guo, Mingyao Li, Stephanie Derohannessian, Paul I W. De Bakker, Swneke D. Bailey, Alexandre Montpetit, Andrew C. Edmondson, Kent Taylor, Xiaowu Gai, Susanna S. Wang, Tamim Shaikh, Leif Groop, Michael Boehnke, Alistair S. Hall, Andrew T. Hattersley, Edward Frackelton, Nick Patterson, Charleston K W Chiang, Cecelia E. Kim, Richard R. Fabsitz, Willem Ouwehand, Alkes L. Price, Patricia Munroe, Mark Caulfield, Thomas Drake, Eric Boerwinkle, David Reich, A Stephen Whitehead, Thomas P. Cappola, Nilesh J. Samani, A Jake Lusis, Eric Schadt, James G. Wilson, Wolfgang Koenig, Mark I. Mccarthy, Sekar Kathiresan, Stacey B. Gabriel, Hakon Hakonarson, Sonia S. Anand, Muredach Reilly, James C. Engert, Deborah A. Nickerson, Daniel J. Rader, Joel N. Hirschhorn, Garret A. Fitzgerald

Faculty, Staff and Student Publications

A wealth of genetic associations for cardiovascular and metabolic phenotypes in humans has been accumulating over the last decade, in particular a large number of loci derived from recent genome wide association studies (GWAS). True complex disease-associated loci often exert modest effects, so their delineation currently requires integration of diverse phenotypic data from large studies to ensure robust meta-analyses. We have designed a gene-centric 50 K single nucleotide polymorphism (SNP) array to assess potentially relevant loci across a range of cardiovascular, metabolic and inflammatory syndromes. The array utilizes a "cosmopolitan" tagging approach to capture the genetic diversity across approximately 2,000 …

Ligustrum Vulgare L., Bret Hash

Specimens by Name

No abstract provided.

2008 Scholars And Artists Bibliography, Rajshehkar G. Javalgi Dr., Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Dr. Rajshekhar Javalgi was the guest speaker.

Usaid/Mali Awards $5 M Associate Cooperative Agreement To Intsormil For Food Insecurity Mitigation In Mali, West Africa, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

Dr. John Yohe, Program Director of the “Sorghum, Millet and other Grains Collaborative Research Support Program” (INTSORMIL) announces receipt from the USAID Mission in Mali, West Africa, of a $5 million grant to expand the activities of the current project “Transfer of Sorghum, Millet Production, Processing and Marketing Technologies in Mali.” Subsistence and resource poor farmers in West Africa are dependent on sorghum and millet as they serve as basic human foods and livestock feed. There continues to be a shortage of sorghum and millet grain in Mali, especially in the north. Mali’s ‘Hungry Season’ has been described as “that …

A Network-Constrained Empirical Bayes Method For Analysis Of Genomic Data, Caiyan Li, Zhi Wei, Hongzhe Li

UPenn Biostatistics Working Papers

Empirical Bayes methods are widely used in the analysis of microarray gene expression data in order to identify the differentially expressed genes or genes that are associated with other general phenotypes. Available methods often assume that genes are independent. However, genes are expected to function interactively and to form molecular modules to affect the phenotypes. In order to account for regulatory dependency among genes, we propose in this paper a network-constrained empirical Bayes method for analyzing genomic data in the framework of general linear models, where the dependency of genes is modeled by a discrete Markov random field model defined …

Smokejumper Obituary: Hammarstrom, Brynolf (Missoula 1943), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Unl Extension Offers Resources For High Inputs In Crop Production, Gary W. Hergert, Thomas Dorn, Sandi Alswager Karstens

Institute of Agriculture and Natural Resources: News Releases

With input costs for the 2009 crop production season projected to be two to three times higher than in recent years, University of Nebraska-Lincoln Extension has resources to help.

UNL's Surviving High Input Costs in Crop Production (http://cropwatch.unl.edu/survivinghighinputcosts.htm) Web page offers Nebraska crop producers timely information to curb increasing costs and improve profit margins.

Input costs, such as fuel, fertilizer, seed and land all have influenced crop production costs, said Tom Dorn, UNL Extension educator in Lancaster County.

"In the last five years, the cost of off-road diesel fuel has shot up from $1.30 to $3.50 per gallon, a cost …

Prevalence Of Hcv And Hiv Infections In 2005-Earthquake-Affected Areas Of Pakistan., Saeed Khan, Mohammad A Rai, Adnan Khan, Amber Farooqui, Shahana U Kazmi, Syed H Ali

Department of Biological & Biomedical Sciences

Background: On October 8, 2005, an earthquake of magnitude 7.6 hit the Northern parts of Pakistan. In the post-earthquake scenario, overcrowding, improper sewage disposal, contamination of food and drinking water, hasty surgical procedures, and unscreened blood transfusions to earthquake victims most likely promotes the spread of infections already prevalent in the area. Objective: The objective of the study reported here was to determine the prevalence of Human Immunodeficiency and Hepatitis C viruses (respectively, HIV and HCV) in the earthquake-affected communities of Pakistan. The samples were analyzed 2 months and then again 11 months after the earthquake to estimate the burden …

Physical Mapping Of Ribosomal Genes In New World Members Of The Genus Chenopodium Using Fluorescence In Situ Hybridization, Maria C. Sederberg

Theses and Dissertations

The genus Chenopodium contains many economically important species in the New World, but is relatively understudied and poorly understood, especially in terms of evolutionary relationships. A better understanding of the structure of this genus could significantly help in breeding efforts on its cultivated members, notably the tetraploid C. quinoa and also certain varieties of C. berlandieri, also tetraploid. Of special concern is determining which diploid weed species are the most likely ancestors for C. quinoa, C. berlandieri, and the other tetraploid members of subsection Cellulata. The phylogeny can be understood in part by examining the ribosomal RNA loci and observing …

Smokejumper Obituary: Padden, William J. (Cave Junction 1948), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Ainsworth, John L. (Missoula 1943), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Putative Gene Promoter Sequences In The Chlorella Viruses, Lisa A. Fitzgerald, Philip Boucher, Giane Yanai-Balser, Karsten Suhre, Michael Graves, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Three short (7 to 9 nucleotides) highly conserved nucleotide sequences were identified in the putative promoter regions (150 bp upstream and 50 bp downstream of the ATG translation start site) of three members of the genus Chlorovirus, family Phycodnaviridae. Most of these sequences occurred in similar locations within the defined promoter regions. The sequence and location of the motifs were often conserved among homologous ORFs within the Chlorovirus family. One of these conserved sequences (AATGACA) is predominately associated with genes expressed early in virus replication.

Forage Spokesman Information, Clayton Geralds, Brady Jarvis

Kentucky Grazing Conference

No abstract provided.

Supplementing Cattle On Pasture: When, What And How Much?, Roy Burris

Kentucky Grazing Conference

The biggest advantage of maintaining cattle in the southeast is our ability to grow forages. It is generally an economic advantage to make maximum use of our forage and then supplement only when needed to meet our production goals. Judicious use of extra nutrition to grazing cattle can allow us to meet an objective which was best stated by Hoveland in 1986:

“Our objective is to maintain pasture at the highest quality that the environment will allow and efficiently convert as much of the pasture as possible into saleable animal product.”

Fencing And Watering Systems: Simpler Is Better, Ralph Quillin

Kentucky Grazing Conference

Sustainability and Grazing Management have taken on a new importance in the Americana farm community. The key components of the sustainable approach, profitability, agronomic, social and ecological responsibility and quality of life are becoming obtainable goals for this and future farming generations.

Practical Grazing Management And Feed Strategies To Alleviate Fescue Toxicosis, Glen Aiken

Kentucky Grazing Conference

Tall fescue grown in Kentucky is productive and persistent and that is why the state has 5 million acres of the stuff! Fescue has tolerances to drought, low fertility, and overgrazing that are attributed to a fungal endophyte which infects most tall fescue plants. Unfortunately, these advantages of tall fescue that livestock producers appreciate are offset by reduced calving percentages and calf weight gains caused by consumption of ergot alkaloids produced by the endophyte. Ergot alkaloids affect the physiology of cattle to cause elevation in body temperature, reduced sweating, retention of winter hair coats and uncontrollable growth of summer hair …

Herbicide Options For Pasture Weed Control, Jonathan D. Green

Kentucky Grazing Conference

Weeds have become more evident and problematic in grazed pastures as forage and livestock production systems have intensified. As animals consume the more desirable and palatable plant species in pastures, weedy plants become more abundant. In particular, animals tend to avoid grazing plants which contain thorns, prickly spines, or are unpalatable making some pastures less productive. In fact, many of the more problematic plants observed in Kentucky’s grazed pastures possess these characteristics including thistles, spiny amaranth (spiny pigweed), horsenettle, common cocklebur, tall ironweed, and buttercups.

Horse Pasture Monitoring Program: Results After Three Years, Tom Keene, S. Ray Smith

Kentucky Grazing Conference

Since 2001-2002 and the outbreak of Mare Reproductive Loss Syndrome (MLRS) the University of Kentucky has been developing stronger ties with the state’s equine industry. Approximately 30% of the foal crop was lost in the Central Bluegrass region during this period with a devastating impact on the industry. Traditionally, many Thoroughbred farms have functioned as independent entities, but MLRS helped them to realize that the University of Kentucky had tremendous amount of expertise that they could provide in the areas of animal health, forage management, and other areas. Initially, there were many theories as to the cause of MLRS with …

Forage Legumes Vs. Fertilizer Nitrogen, Garry D. Lacefield

Kentucky Grazing Conference

A few weeks after I started to work as a Forage Extension Specialist at the University of Kentucky, I heard my mentor and forage idol, Mr. Warren Thompson, speak on forage legumes. Warren, along with Dr’s. Tim Taylor and W.C. Templeton had pioneered the “Pasture Renovation Program” in Kentucky. In addition, Mr. Ed Smith had joined the team and collectively they developed the “Power-til Pasture Renovator.” Warren began his presentation by talking about Kentucky Forages pointing out we were so fortunate to have a good grass base and the opportunity to grow lots of legumes. He also talked about all …

Growth Of Grasses And Legumes: Impact On Grazing, S. Ray Smith

Kentucky Grazing Conference

The primary, cool season, perennial pasture grasses used in Kentucky are orchardgrass, bluegrass, and tall fescue. When not grazed or harvested, each of these passes through successive stages of growth in the spring: 1) leafy vegetative; 2) boot with seed heads enclosed in leaf sheath; 3) heading when the seed heads begin to show and, 4) bloom when pollination has occurred ( Figure 2). Since fiber and lignin contents increase steadily beyond the vegetative stage, while percent protein and digestibility decrease, a major goal in grazing management is to maintain these grasses in the leafy, vegetative stage at all times. …

Foreword And Kfgc Award Winners [2008], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Adaptive Management Report For The Clark County, Nevada Multiple Species Habitat Conservation Plan, Desert Conservation Program

Publications (C)

The Desert Conservation Program administers an incidental take permit for desert tortoise and 77 other species. The permit was issued in 2001 by the United States Fish and Wildlife Service in accordance with the Endangered Species Act. Compliance with the permit requires implementation of the Clark County Multiple Species Habitat Conservation Plan. This plan requires an adaptive management program and a biennial report by an independent science advisor to assess the status of the plan and make recommendations for future funding. The 2008 Adaptive Management Report reviews current data on land use trends, habitat loss, species status, plan implementation, programmatic …

Impact Of Seated And Standing Bicycle Riding Position On Subsequent Running Performance, Randall L. Jensen‡, Brendon J. Ewers†, Brian Cunniffe*, Brian Phelan*, Andrew J. Harrison‡, Amir Shafat‡

International Journal of Exercise Science

This study examined the effects of cycling posture on subsequent running performance similar to the transition phase of a triathlon. Experienced, non-elite triathletes completed two trials of a cycle-run transition. During the last three minutes of a 30 minute cycling bout, at power output equal to lactate threshold, subjects either remained seated (SEAT), or alternated seated and standing cycling (30 s at a time; ALT). Heart rate, RPE, minimum and maximum knee angle, stride frequency and length, and onset and duration of quadriceps and hamstrings activity were obtained at the end of a three-minute control run and at minutes 0, …

Estimating Kinetic And Thermodynamic Parameters From Single Molecule Enzyme-Inhibitor Interactions, Laura Porter-Peden, Sarah G. Kamper, Mark Vander Wal, Ronald Blankespoor

University Faculty Publications and Creative Works

We report the application of recently developed microscopic models to estimate the apparent kinetic and thermodynamic parameters in a single molecule force spectroscopy study of the carbonic anhydrase enzyme and a complementary sulfonamide inhibitor. The most probable rupture force for the enzyme-inhibitor interaction shows a nonlinear dependency on the log-loading rate. Estimates for the kinetic and thermodynamic parameters were obtained by fitting the nonlinear dependency to linear cubic potential and cusp potential models and compared to the standard Bell-Evans model. The reliability of the estimated parameters was verified by modeling the experimental rupture force distributions by the theoretically predicted distributions …

Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, Jennifer D. Cotter, Andrew Storfer, Robert B. Page, Christopher K. Beachy, S. Randal Voss

Biology Faculty Publications

Background

Very little is known about the immunological responses of amphibians to pathogens that are causing global population declines. We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV).

Result

At 0, 24, 72, and 144 hours post-infection, spleen and lung samples were removed for estimation of host mRNA abundance and viral load. A total of 158 up-regulated and 105 down-regulated genes were identified across all time points using statistical and fold level criteria. The presumptive functions of these genes suggest a robust …

On Measuring Progress In Animal Welfare, James A. Serpell

Assessment of Animal Welfare Collection

Improvements in the welfare of animals arise from two primary sources: Changes in public attitudes and beliefs regarding what constitutes acceptable treatment of animals, and mandatory changes in behavior toward animals imposed by governments and regulatory authorities. During the last 30-40 years, public and political support for improvements in animal welfare has increased dramatically in many countries. Funding from both governmental and non-governmental sources continues to grow, and the relatively new field of animal welfare science has emerged and expanded to support and inform these efforts. Many experts in the field recognize, however, that there is still a distinct gap …

Relationship Web: Trailblazing, Analytics And Computing For Human Experience, Amit P. Sheth

Kno.e.sis Publications

This panel presentation was give at the 27th International Conference on Conceptual Modeling (ER 2008), Barcelona, Spain, October 20-23, 2008.

Viral Vectors In The Research Laboratory: Just How Safe Are They?, Dawn P. Wooley, Kimberly Kay Morris, Robert Mcrae, John C. Trefry

Neuroscience, Cell Biology & Physiology Faculty Publications

No abstract provided.

Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, Jennifer D. Cotter, Andrew Storfer, Robert B. Page, Christopher K. Beachy, S. Randal Voss

Biology Faculty Publications

BACKGROUND: Very little is known about the immunological responses of amphibians to pathogens that are causing global population declines. We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV).

RESULT: At 0, 24, 72, and 144 hours post-infection, spleen and lung samples were removed for estimation of host mRNA abundance and viral load. A total of 158 up-regulated and 105 down-regulated genes were identified across all time points using statistical and fold level criteria. The presumptive functions of these genes suggest a robust innate immune …